pycom07g09080

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008059
Physical Location & Seq
Reverse (-)
8252473 .. 8253012
540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGATGAAGGGCATTGTGATCTCAAAGTTGGTTCTGCTAGCCATGGCTCACCTAATGGTGAAGCAAGGAGAGGCCGTCGTCTCTTGTGGCCAGGTGGACTCGAACTTAGCGCCTTGCGTTTCCTACCTGACCAAACGGGGTAATCTTGACTCGGCTTACTGCACAGGGGTGCAGAACATCAAGAACCTCGCCCAAACCACAGCTGATAAGCAGGCCGCTTGTGAATGTTGTAAGGCAGCGGCAAACTGCTTCCCCAGTGTTGACGAAGTTACAGCTTCTACTCTCCCAGGCAAGTGTGGGATTGTGATGATTATCCCCATCTCAAAGACCACCAACTGCCAGCGGCGAGTCCAAGATGGAGGTGATCAAGGGCAGGAAATAAAGCAAGAGAAAGCTTTGAGTGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

137

Amino Acids

14.38

Weight (kDa)

8.38

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 29 - 103 2.2e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 216, 239, 343
AcoI YGGCCR 1 cut(s) 88
AjnI CCWGG 2 cut(s) 90, 286
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 3 cut(s) 203, 275, 395
AluI AGCT 3 cut(s) 203, 275, 395
Alw26I GTCTC 1 cut(s) 85
AoxI GGCC 3 cut(s) 72, 88, 213
ApeKI GCWGC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 41, 70, 374
AsuNHI GCTAGC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 90
BbvI GCAGC 1 cut(s) 248
BccI CCATC 2 cut(s) 326, 350
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 2 cut(s) 92, 288
BclI TGATCA 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 1 cut(s) 113
BisI GCNGC 4 cut(s) 216, 237, 240, 344
BlsI GCNGC 4 cut(s) 217, 238, 241, 345
Bme1390I CCNGG 2 cut(s) 92, 288
BmrFI CCNGG 2 cut(s) 92, 288
BmrI ACTGGG 1 cut(s) 249
BmtI GCTAGC 1 cut(s) 41
BmuI ACTGGG 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 42, 286
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bse1I ACTGG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 2 cut(s) 92, 288
BseDI CCNNGG 2 cut(s) 42, 286
BseJI GATNNNNATC 1 cut(s) 311
BseNI ACTGG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 248
BsgI GTGCAG 2 cut(s) 145, 191
BshFI GGCC 3 cut(s) 74, 90, 215
BsmAI GTCTC 1 cut(s) 85
BsmBI CGTCTC 1 cut(s) 85
BsnI GGCC 3 cut(s) 74, 90, 215
Bsp143I GATC 2 cut(s) 18, 364
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 3 cut(s) 216, 239, 343
BspANI GGCC 3 cut(s) 74, 90, 215
BspOI GCTAGC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 42, 286
BssMI GATC 2 cut(s) 18, 364
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 2 cut(s) 92, 288
BstC8I GCNNGC 3 cut(s) 39, 213, 341
BstDEI CTNAG 1 cut(s) 106
BstDSI CCRYGG 1 cut(s) 42
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 21, 367
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 18, 364
BstNI CCWGG 2 cut(s) 92, 288
BstSCI CCNGG 2 cut(s) 90, 286
BstV1I GCAGC 1 cut(s) 248
BsuRI GGCC 3 cut(s) 74, 90, 215
BtgI CCRYGG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 39, 213, 341
CfoI GCGC 1 cut(s) 112
CviAII CATG 1 cut(s) 43
CviJI RGCY 9 cut(s) 41, 47, 74, 90, 155, 203, 215, 275, 395
CviKI_1 RGCY 9 cut(s) 41, 47, 74, 90, 155, 203, 215, 275, 395
DdeI CTNAG 1 cut(s) 106
DpnI GATC 2 cut(s) 20, 366
DpnII GATC 2 cut(s) 18, 364
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 42
EcoRII CCWGG 2 cut(s) 90, 286
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 1 cut(s) 46
FaiI YATR 3 cut(s) 44, 407, 409
FatI CATG 1 cut(s) 42
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 4 cut(s) 216, 237, 240, 344
Fsp4HI GCNGC 4 cut(s) 216, 237, 240, 344
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 111
GluI GCNGC 4 cut(s) 216, 237, 240, 344
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 3 cut(s) 74, 90, 215
HhaI GCGC 1 cut(s) 112
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 3 cut(s) 98, 149, 348
HphI GGTGA 3 cut(s) 41, 70, 374
Hpy166II GTNNAC 2 cut(s) 97, 262
Hpy188III TCNNGA 2 cut(s) 146, 181
Hpy8I GTNNAC 2 cut(s) 97, 262
Hpy99I CGWCG 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 162, 172
HpyF3I CTNAG 1 cut(s) 106
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 2 cut(s) 18, 364
Lsp1109I GCAGC 1 cut(s) 248
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 268
MalI GATC 2 cut(s) 20, 366
MboI GATC 2 cut(s) 18, 364
MlsI TGGCCA 1 cut(s) 90
MluNI TGGCCA 1 cut(s) 90
MlyI GAGTC 3 cut(s) 92, 143, 357
MnlI CCTC 3 cut(s) 64, 197, 353
Mox20I TGGCCA 1 cut(s) 90
MscI TGGCCA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 3 cut(s) 203, 239, 343
MspR9I CCNGG 2 cut(s) 92, 288
MvaI CCWGG 2 cut(s) 92, 288
NcoI CCATGG 1 cut(s) 42
NdeII GATC 2 cut(s) 18, 364
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 1 cut(s) 46
NmeAIII GCCGAG 1 cut(s) 131
OliI CACNNNNGTG 1 cut(s) 167
PkrI GCNGC 4 cut(s) 217, 238, 241, 345
PleI GAGTC 3 cut(s) 92, 143, 356
PpsI GAGTC 3 cut(s) 92, 143, 356
Psp6I CCWGG 2 cut(s) 90, 286
PspGI CCWGG 2 cut(s) 90, 286
PvuII CAGCTG 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 167
SatI GCNGC 4 cut(s) 216, 237, 240, 344
Sau3AI GATC 2 cut(s) 18, 364
SchI GAGTC 3 cut(s) 92, 143, 357
ScrFI CCNGG 2 cut(s) 92, 288
SetI ASST 8 cut(s) 54, 96, 129, 189, 205, 277, 364, 397
SmiMI CAYNNNNRTG 1 cut(s) 167
SsiI CCGC 3 cut(s) 216, 239, 343
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 90, 286
StyI CCWWGG 1 cut(s) 42
TaqI TCGA 1 cut(s) 101
TauI GCSGC 3 cut(s) 218, 242, 346
TscAI CASTG 1 cut(s) 262
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 262
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.