pycom07g09730

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005282
Physical Location & Seq
Forward (+)
9106095 .. 9117480
11386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4107 bp
ATGCTCAATTTTACGCCGACCACCGCCAGCCAGATTCGCTTCCTACTCCAGAGCCTCAACGATGCCACCTCCGACTCCGTTCTCCGAGAGCTCTCTCAGTTTACTGAATATGGACTTGAGGGAAGCATTTTGCTGCTACAGACCTGTCTGGATCATCTCACTAATTACAGAACAGACTTAAAGAACGCAGCATTGGAACGAGTTCTTGCCTCAATTTTCAAATATCTCCTTGACAGACCTAATTTCAGTACAGTGTTTTGTGAGTGCCTAAGAAATACAGAGATTAATGAAGGAATTCTTGAGAATTTCTCTAATGCATTACACCTATCTGTATCGGAGAAAATTAGCATTGCTCTTGCGCTGTCAGATTCTGAAAACCTAGACACCAGGATATGTGGCAAGAACTTCTGTATGGATCAGATCCAGAAATTGTGTGAAAGTCCTATTGCTATGAATTCGTCTGAGCAAATTCAGAACATTGTTATATTCCTCCAGCGGTCTGAGGGCCTTTCCAAGCATGTGGATTCCTTTATGCAGATGTTATCTTTACTGCAACTGAAAGATGACAGTTTTTCTGTTTTAACTCCATTGCTTTCAGATGAGCTCCGTGATGCCAATTTTTTGAGCAATGTGGGTTTGTTGCATGAAAGCGGAGAAAATGATTTTGATGCTATTTTAGCTGAAATGGCGAAGGAAATCAGCATGGGAGATGTAATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAAAGAGATTTTATCTCTTTTCTTACCACTCACTGAGATTACTATTTCAAAAATACTTGGCATGATTGCCTCTACTCATGTTGGTCTCGAGGACAATCAGAATGCATTTTCAACATTCCGGTTGGCGGTTGGTAACAGCACTTTATCTGATCTGCCTATGTTGAACACCTGGAATATTGATGTCCTTGTAGATACAATCAAGCAACTTGCTCCCGGTACTAACTGGATACAAGTTATTGAAAATTTGGATCATGAGGGATTCTTCATTCCTAATCAGGAGGCATTCTCTTTTTTCATGTCTGTATATCAGCATGTCTGTCAGGAGCTGTTCCCCCTCCATGTTATCTGCGGGTCTGTTTGGAAGAACACCGAGGGTCAGCTATCCTTCCTTAAACATGCTGTGTCAGCACCACCTGAAGTGTTTACCTTTGCCCACTCTGTTAGGCAGCTGGCCTATATTGATGCAGTGCATGGCCATAAGCTTCAACTTGGACATGCAAATCATGCATGGTTGAGTCTTGACCTTTTGGATGTACTGTGTCTACTAGCTGAGAGGGGTCATGCTCTTGCTGACCGATCAATGCTTGAGTATCCTCTTAAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCGCATACAAATACTGCTTATAACCTGCTGCAATATGAAGTGTCTTATACTGTTTTCCCCATGATGGTAAAAAATGCCATGGGCATTGGCATGATTAACCACCTCTGGCATATTAACACCTCATTGGTGTTGAGGGGATTTGTGGATGCTCACAATTGTGATCCAGATAGCATAGCTAGAATTTTGGATATCTGTCAAGAGCTAAAGATTCTGTCATCTGTCCTGGAGATGATCCCTTCCTCTTTAAGTATCAGATTGGCAGCCCTTGCTTCTAAAAAAGAGCTTGTAGACCTTGAGAAGTGGTTGAGTAATAATTTGAATTTGTATAAGGATACTTTCTTTGAGGAGTGCATAAAGTTCTTGAAGGAGATTCAATTTGGTGGTTCACAGGATTTTTCCACCAGACCTTTCCAGCATTCTGGTGCCGTGTCTAATATTTATGTGGACACTGCTACTATCTTCACGCAGGTTCTTAAAGCTCATGTTGGTTTGGTTGCCTCTAGCCAACTTACTGAGGAGATGGAAAGGTTGTCTGTAACAATTATGGATTCGATTCCAAGGCTCCCGAATGGTGGCACGACAGAGTCTTCAACTGATGGATATGCAGATGATATTGAAGCTGAAGCAAACTCTTACTTCCATCAAATGTTTTCTGGTCAATTGACAATTGATTCAATGGTCCAAATGCTTGCCCGATTCAAGGAATCTTCTGTAAAAAGGGAACAGTCAATTTTTGAGTGTATGATTGCAAATCTGTTTGAAGAGTATAGATTCTTCCCCAAGTATCCTGAAAGACAGCTCAAACTTGCTGCAGTTCTGTTTGGCTCTGTCATCAAGCACCAGCTCGTAACTCATCTGACTCTTGGGATTGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAGTTTGTTTGTGTTCGGAACAAAGGCCTTGGAGCAGTTTGTGGATCGATCTGATTGAGTGGCCGCAGTATTGCAATCATATTCTTCAAATATCCCATCTGCGGAGTACTCATTCAGAGCTTGTGGCTTTCATTGAACAGGCTCTTGCCAGAATTTCTTCAAGCCATTCAGACTCGGATGGAAGTAACCATGCTTCTGCTGCTCATCACCATGGTCCCAGTCAAGCTACCCCAGGAAATGTGGAGTTAAATGGTTCTACCATCTTACATAATGGGCAACAACATTCTTCTCCACTCCAGCTTCAACAGAGACATGAAAGTTCTCTTGATGACCGTCATAAAGCCTCGAATTCATCAAACGACATAAAGCCACCTTTATCTTCTGCTGTGCAACCTCCGGTTGGTCCTGTAGGCGATACTCCTCGTATTCAGAAGTCACAAAGTGCACTCAGTGCTCCGGCAATGTTGTCTTCTCCTTCCCCTGGTTTCGTTCGTCCTACGCGAGGAGTTACTTCTACCAGGTTTGGCTCTGCTTTGAATATTGAAACATTAGTTGCTGCTGCCGAGAAAAGAGATACTCTTATTGAGGCTCCAGCATCAGAGATTCAGGACAAGATATCATTTATAATTAATAATATTTCAGTTGCCAATGTTGAAGCTAAGTCAAAGGAGTTTGCTGAAATAATCAAAGAGCAGTACTATCCTTGGTTTGCACAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCGAATTTTCATGATTTGTACCTGAAATTTCTGGACAAAGTTAATTCCAAGGGTTTGAATAAGGAGATTGTTCAAGCCACTTATGAGAACTGCAAGGTTCTTCTTGGATCTGAGCTTATAAAGTCAAGCGCAGAAGAGCGTTCGTTGCTAAAAAATTTAGGTAGCTGGCTTGGGAAGCTTACAATTGGAAGGAATCAAGTCTTAAGGGCTCGGGAAATAGATCCCAAATCTTTGATTATAGAGGCATATGAGAAAGGGTTGATGATTGCAGTTATACCCTTTACTTCAAAGATTCTGGAGCCATGCCAAAGTAGCTTGGCATATCAGCCACCAAATCCTTGGACAATGGGTATTCTTGGATTACTTGCTGAGATATATTCAATGCCAAACTTGAAAATGAACCTGAAGTTTGACATCGAGGTTCTATTTAAGAACCTTGGTGTGGATTTGAAGGAGATTACCCCAAGTTCTCTACTCAAGGACCGGAACAGAGAAGTTGAAGGGAATCCTGATTTCTCTAACAAAGATATTGGAGCATCCCAACCACAGATGGTTGCCGAAGTGAAACTAAATCAAGTTGAACTACCAGTTGATGTTGGTCCATCTAATTCTGCAAGCCATACACATATATCTCAGCTACTCCATCCCAGTCACCATTCTCTGTTACTTCCCACACCAATATCTAATATTGCAACTCACGTGGTTATCAACCCGAAACTCAGTGCTTTGGGATTGCACTTGCATTTTCAGAGGGTGGTTCCAATTGCAATGGACAGAGCTATCAAAGAGATAGTAACTGGTATCGTTCAGCGCAGTGTTTCTATAGCTACTCAGACAACTAAAGAGCTTGTCTTAAAGGATTATGCCATGGAATCCGATGAGACAAGAATATTACTGGCAGCTCACTTGATGGTTGCAAGTTTGGCAGGAAGTCTAGCTCATGTAACTTGCAAGGAACCCTTGCGTAGTTCAATATCAACCCAGCTGCGGAATTCCCTTCAGGGATTAAATATTCCAAATGATCGATCTGGAACATGCTGTACAACTTGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

1369

Amino Acids

152.06

Weight (kDa)

5.71

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 477 - 622 1.4e-34 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 657 - 822 4.2e-56 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 963 - 1183 3.7e-90 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1265 - 1358 1.5e-33 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1410, 2930, 3170
Acc16I TGCGCA 1 cut(s) 2288
Acc36I ACCTGC 3 cut(s) 1422, 1846, 2302
AccB1I GGYRCC 1 cut(s) 1811
AccI GTMKAC 2 cut(s) 1300, 1677
AccII CGCG 1 cut(s) 2806
AciI CCGC 9 cut(s) 24, 496, 651, 884, 1107, 2297, 2369, 2407, 4039
AclWI GGATC 9 cut(s) 159, 415, 423, 1014, 1544, 1615, 2357, 3166, 3266
AcoI YGGCCR 2 cut(s) 1231, 2366
AcuI CTGAAG 5 cut(s) 1194, 1389, 2031, 3476, 4034
AcvI CACGTG 1 cut(s) 3751
AdeI CACNNNGTG 2 cut(s) 2747, 2756
AfaI GTAC 7 cut(s) 250, 976, 1293, 2413, 3002, 3071, 4093
AflII CTTAAG 1 cut(s) 3253
AjnI CCWGG 6 cut(s) 386, 926, 1611, 2536, 2785, 2822
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 1 cut(s) 1545
Alw21I GWGCWC 4 cut(s) 93, 606, 2752, 2761
Alw26I GTCTC 3 cut(s) 848, 2608, 3926
Alw44I GTGCAC 1 cut(s) 2748
AlwI GGATC 9 cut(s) 159, 415, 423, 1014, 1544, 1615, 2357, 3166, 3266
AlwNI CAGNNNCTG 2 cut(s) 371, 1084
Ama87I CYCGRG 2 cut(s) 845, 3261
AoxI GGCC 5 cut(s) 505, 1209, 1231, 2330, 2366
ApaLI GTGCAC 1 cut(s) 2748
AseI ATTAAT 2 cut(s) 285, 2934
Asp700I GAANNNNTTC 3 cut(s) 201, 294, 2461
AspLEI GCGC 5 cut(s) 361, 1393, 2289, 3182, 3864
AspS9I GGNCC 6 cut(s) 505, 2068, 2519, 2708, 3532, 3650
AsuC2I CCSGG 1 cut(s) 972
AsuHPI GGTGA 2 cut(s) 2504, 3695
AvaI CYCGRG 2 cut(s) 845, 3261
AvaII GGWCC 5 cut(s) 2068, 2519, 2708, 3532, 3650
BaeGI GKGCMC 1 cut(s) 2752
BalI TGGCCA 1 cut(s) 1233
BanI GGYRCC 1 cut(s) 1811
BanII GRGCYC 3 cut(s) 93, 606, 3262
BarI GAAGNNNNNNTAC 4 cut(s) 2147, 2179, 2482, 2514
BbrPI CACGTG 1 cut(s) 3751
BbsI GAAGAC 2 cut(s) 1968, 2766
Bbv12I GWGCWC 4 cut(s) 93, 606, 2752, 2761
BceAI ACGGC 1 cut(s) 1799
BciT130I CCWGG 6 cut(s) 388, 928, 1613, 2538, 2787, 2824
BciVI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BcnI CCSGG 1 cut(s) 972
BcoDI GTCTC 3 cut(s) 848, 2608, 3926
BfaI CTAG 5 cut(s) 380, 1304, 1566, 1890, 3986
BfmI CTRYAG 4 cut(s) 137, 2199, 2712, 3873
BfrI CTTAAG 1 cut(s) 3253
BfuAI ACCTGC 3 cut(s) 1422, 1846, 2302
BfuI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BmcAI AGTACT 2 cut(s) 2413, 3002
Bme1390I CCNGG 7 cut(s) 388, 928, 972, 1613, 2538, 2787, 2824
Bme18I GGWCC 5 cut(s) 2068, 2519, 2708, 3532, 3650
BmeT110I CYCGRG 2 cut(s) 845, 3261
BmgT120I GGNCC 6 cut(s) 505, 2068, 2519, 2708, 3532, 3650
BmiI GGNNCC 7 cut(s) 1813, 1952, 2521, 2895, 3351, 3810, 4008
BmrFI CCNGG 7 cut(s) 388, 928, 972, 1613, 2538, 2787, 2824
BmrI ACTGGG 2 cut(s) 2517, 3693
BmsI GCATC 9 cut(s) 52, 601, 658, 725, 1210, 1525, 2269, 2909, 3596
BmuI ACTGGG 2 cut(s) 2517, 3693
BpiI GAAGAC 2 cut(s) 1968, 2766
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 1028, 1060
BpmI CTGGAG 6 cut(s) 32, 476, 1634, 2585, 2880, 3368
BpuEI CTTGAG 5 cut(s) 137, 320, 1364, 1703, 3512
BpuMI CCSGG 1 cut(s) 972
Bsa29I ATCGAT 2 cut(s) 2352, 4075
BsaAI YACGTR 1 cut(s) 3751
BsaI GGTCTC 1 cut(s) 848
BsaWI WCCGGW 3 cut(s) 876, 2701, 3534
Bse1I ACTGG 6 cut(s) 986, 2523, 3638, 3699, 3853, 3951
Bse3DI GCAATG 6 cut(s) 348, 587, 634, 2282, 2772, 3825
BseBI CCWGG 6 cut(s) 388, 928, 1613, 2538, 2787, 2824
BseCI ATCGAT 2 cut(s) 2352, 4075
BseGI GGATG 7 cut(s) 740, 1294, 1392, 1540, 2488, 3587, 3694
BseMI GCAATG 6 cut(s) 348, 587, 634, 2282, 2772, 3825
BseNI ACTGG 6 cut(s) 986, 2523, 3638, 3699, 3853, 3951
BseRI GAGGAG 4 cut(s) 1748, 1919, 2715, 2823
BseSI GKGCMC 1 cut(s) 2752
BseYI CCCAGC 1 cut(s) 4032
Bsh1236I CGCG 1 cut(s) 2806
BshFI GGCC 5 cut(s) 507, 1211, 1233, 2332, 2368
BshNI GGYRCC 1 cut(s) 1811
BshVI ATCGAT 2 cut(s) 2352, 4075
BsiHKAI GWGCWC 4 cut(s) 93, 606, 2752, 2761
BsiHKCI CYCGRG 2 cut(s) 845, 3261
BsiSI CCGG 5 cut(s) 877, 972, 2702, 2762, 3535
BslFI GGGAC 1 cut(s) 2505
BsmAI GTCTC 3 cut(s) 848, 2608, 3926
BsmFI GGGAC 1 cut(s) 2505
BsmI GAATGC 3 cut(s) 865, 1040, 1804
BsnI GGCC 5 cut(s) 507, 1211, 1233, 2332, 2368
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 2 cut(s) 845, 3261
Bsp1286I GDGCHC 5 cut(s) 93, 606, 2752, 2761, 3262
Bsp1407I TGTACA 1 cut(s) 4091
Bsp19I CCATGG 3 cut(s) 1467, 2515, 3918
BspACI CCGC 9 cut(s) 24, 496, 651, 884, 1107, 2297, 2369, 2407, 4039
BspANI GGCC 5 cut(s) 507, 1211, 1233, 2332, 2368
BspDI ATCGAT 2 cut(s) 2352, 4075
BspFNI CGCG 1 cut(s) 2806
BspHI TCATGA 2 cut(s) 1009, 3061
BspLI GGNNCC 7 cut(s) 1813, 1952, 2521, 2895, 3351, 3810, 4008
BspMAI CTGCAG 1 cut(s) 2203
BspMI ACCTGC 3 cut(s) 1422, 1846, 2302
BspPI GGATC 9 cut(s) 159, 415, 423, 1014, 1544, 1615, 2357, 3166, 3266
BspQI GCTCTTC 1 cut(s) 3180
BspT107I GGYRCC 1 cut(s) 1811
BspTI CTTAAG 1 cut(s) 3253
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 6 cut(s) 348, 587, 634, 2282, 2772, 3825
BsrGI TGTACA 1 cut(s) 4091
BsrI ACTGG 6 cut(s) 986, 2523, 3638, 3699, 3853, 3951
BssT1I CCWWGG 9 cut(s) 1467, 1946, 2333, 2515, 3008, 3099, 3389, 3487, 3918
Bst2UI CCWGG 6 cut(s) 388, 928, 1613, 2538, 2787, 2824
Bst4CI ACNGT 9 cut(s) 253, 569, 742, 1296, 1364, 1441, 2115, 2639, 3021
Bst6I CTCTTC 2 cut(s) 2145, 3180
BstAFI CTTAAG 1 cut(s) 3253
BstAPI GCANNNNNTGC 3 cut(s) 1262, 1655, 2756
BstAUI TGTACA 1 cut(s) 4091
BstBAI YACGTR 1 cut(s) 3751
BstC8I GCNNGC 6 cut(s) 28, 1209, 2079, 3043, 3218, 3667
BstDSI CCRYGG 3 cut(s) 1467, 2515, 3918
BstENI CCTNNNNNAGG 1 cut(s) 2805
BstF5I GGATG 7 cut(s) 740, 1294, 1392, 1540, 2488, 3587, 3694
BstFNI CGCG 1 cut(s) 2806
BstHHI GCGC 5 cut(s) 361, 1393, 2289, 3182, 3864
BstMAI GTCTC 3 cut(s) 848, 2608, 3926
BstNI CCWGG 6 cut(s) 388, 928, 1613, 2538, 2787, 2824
BstNSI RCATGY 5 cut(s) 521, 1073, 1157, 1256, 4089
BstSCI CCNGG 7 cut(s) 386, 926, 970, 1611, 2536, 2785, 2822
BstSFI CTRYAG 4 cut(s) 137, 2199, 2712, 3873
BstSLI GKGCMC 1 cut(s) 2752
BstUI CGCG 1 cut(s) 2806
BstV2I GAAGAC 2 cut(s) 1968, 2766
BstX2I RGATCY 3 cut(s) 420, 3158, 3271
BstXI CCANNNNNNTGG 3 cut(s) 520, 1808, 3390
BstYI RGATCY 3 cut(s) 420, 3158, 3271
Bsu15I ATCGAT 2 cut(s) 2352, 4075
BsuI GTATCC 4 cut(s) 978, 1359, 1714, 2184
BsuRI GGCC 5 cut(s) 507, 1211, 1233, 2332, 2368
BsuTUI ATCGAT 2 cut(s) 2352, 4075
BtgI CCRYGG 3 cut(s) 1467, 2515, 3918
BtsCI GGATG 7 cut(s) 740, 1294, 1392, 1540, 2488, 3587, 3694
BtsI GCAGTG 3 cut(s) 1230, 1836, 3871
BtsIMutI CAGTG 9 cut(s) 258, 761, 789, 1230, 1360, 1836, 2761, 3778, 3871
BveI ACCTGC 3 cut(s) 1422, 1846, 2302
Cac8I GCNNGC 6 cut(s) 28, 1209, 2079, 3043, 3218, 3667
CaiI CAGNNNCTG 2 cut(s) 371, 1084
CciI TCATGA 2 cut(s) 1009, 3061
CfoI GCGC 5 cut(s) 361, 1393, 2289, 3182, 3864
Cfr13I GGNCC 6 cut(s) 505, 2068, 2519, 2708, 3532, 3650
ClaI ATCGAT 2 cut(s) 2352, 4075
CsiI ACCWGGT 1 cut(s) 2822
Csp6I GTAC 7 cut(s) 249, 975, 1292, 2412, 3001, 3070, 4092
CspCI CAANNNNNGTGG 2 cut(s) 3732, 3767
CviQI GTAC 7 cut(s) 249, 975, 1292, 2412, 3001, 3070, 4092
DraIII CACNNNGTG 2 cut(s) 2747, 2756
EaeI YGGCCR 2 cut(s) 1231, 2366
Eam1104I CTCTTC 2 cut(s) 2145, 3180
EarI CTCTTC 2 cut(s) 2145, 3180
Ecl136II GAGCTC 2 cut(s) 91, 604
Eco130I CCWWGG 9 cut(s) 1467, 1946, 2333, 2515, 3008, 3099, 3389, 3487, 3918
Eco147I AGGCCT 1 cut(s) 2332
Eco24I GRGCYC 3 cut(s) 93, 606, 3262
Eco31I GGTCTC 1 cut(s) 848
Eco32I GATATC 2 cut(s) 1579, 2922
Eco47I GGWCC 5 cut(s) 2068, 2519, 2708, 3532, 3650
Eco53kI GAGCTC 2 cut(s) 91, 604
Eco57I CTGAAG 5 cut(s) 1194, 1389, 2031, 3476, 4034
Eco72I CACGTG 1 cut(s) 3751
Eco88I CYCGRG 2 cut(s) 845, 3261
EcoICRI GAGCTC 2 cut(s) 91, 604
EcoNI CCTNNNNNAGG 1 cut(s) 2805
EcoO109I RGGNCCY 1 cut(s) 505
EcoRI GAATTC 4 cut(s) 294, 454, 2653, 4042
EcoRII CCWGG 6 cut(s) 386, 926, 1611, 2536, 2785, 2822
EcoRV GATATC 2 cut(s) 1579, 2922
EcoT14I CCWWGG 9 cut(s) 1467, 1946, 2333, 2515, 3008, 3099, 3389, 3487, 3918
EcoT22I ATGCAT 4 cut(s) 319, 865, 1267, 2284
EcoT38I GRGCYC 3 cut(s) 93, 606, 3262
ErhI CCWWGG 9 cut(s) 1467, 1946, 2333, 2515, 3008, 3099, 3389, 3487, 3918
FalI AAGNNNNNCTT 6 cut(s) 282, 314, 1362, 1394, 3995, 4027
FaqI GGGAC 1 cut(s) 2505
FauI CCCGC 1 cut(s) 1100
FauNDI CATATG 1 cut(s) 3298
FblI GTMKAC 2 cut(s) 1300, 1677
FokI GGATG 7 cut(s) 747, 1301, 1399, 1547, 2495, 3574, 3681
FriOI GRGCYC 3 cut(s) 93, 606, 3262
FspBI CTAG 5 cut(s) 380, 1304, 1566, 1890, 3986
FspI TGCGCA 1 cut(s) 2288
GlaI GCGC 5 cut(s) 360, 1392, 2288, 3181, 3863
GsaI CCCAGC 1 cut(s) 4036
GsuI CTGGAG 6 cut(s) 32, 476, 1634, 2585, 2880, 3368
HaeIII GGCC 5 cut(s) 507, 1211, 1233, 2332, 2368
HapII CCGG 5 cut(s) 877, 972, 2702, 2762, 3535
HhaI GCGC 5 cut(s) 361, 1393, 2289, 3182, 3864
Hin6I GCGC 5 cut(s) 359, 1391, 2287, 3180, 3862
HinP1I GCGC 5 cut(s) 359, 1391, 2287, 3180, 3862
HindIII AAGCTT 2 cut(s) 1238, 3227
HpaII CCGG 5 cut(s) 877, 972, 2702, 2762, 3535
HphI GGTGA 2 cut(s) 2504, 3695
Hpy166II GTNNAC 7 cut(s) 102, 1182, 1301, 1678, 1775, 1834, 2750
Hpy8I GTNNAC 7 cut(s) 102, 1182, 1301, 1678, 1775, 1834, 2750
HpyCH4III ACNGT 9 cut(s) 253, 569, 742, 1296, 1364, 1441, 2115, 2639, 3021
HpyCH4IV ACGT 1 cut(s) 3750
HpySE526I ACGT 1 cut(s) 3750
HspAI GCGC 5 cut(s) 359, 1391, 2287, 3180, 3862
LguI GCTCTTC 1 cut(s) 3180
LmnI GCTCC 9 cut(s) 609, 973, 1081, 1956, 2337, 2764, 2899, 3349, 3584
LweI GCATC 9 cut(s) 52, 601, 658, 725, 1210, 1525, 2269, 2909, 3596
MabI ACCWGGT 1 cut(s) 2822
MaeI CTAG 5 cut(s) 380, 1304, 1566, 1890, 3986
MaeII ACGT 1 cut(s) 3750
MfeI CAATTG 5 cut(s) 1543, 2048, 2055, 3234, 3813
MflI RGATCY 3 cut(s) 420, 3158, 3271
MhlI GDGCHC 5 cut(s) 93, 606, 2752, 2761, 3262
MlsI TGGCCA 1 cut(s) 1233
MluNI TGGCCA 1 cut(s) 1233
MlyI GAGTC 5 cut(s) 68, 1282, 1982, 2242, 2471
MmeI TCCRAC 1 cut(s) 96
Mox20I TGGCCA 1 cut(s) 1233
Mph1103I ATGCAT 4 cut(s) 319, 865, 1267, 2284
MroXI GAANNNNTTC 3 cut(s) 201, 294, 2461
MscI TGGCCA 1 cut(s) 1233
MslI CAYNNNNRTG 5 cut(s) 754, 1478, 1545, 1809, 2514
Msp20I TGGCCA 1 cut(s) 1233
MspA1I CMGCKG 3 cut(s) 496, 1207, 4036
MspCI CTTAAG 1 cut(s) 3253
MspI CCGG 5 cut(s) 877, 972, 2702, 2762, 3535
MspR9I CCNGG 7 cut(s) 388, 928, 972, 1613, 2538, 2787, 2824
MunI CAATTG 5 cut(s) 1543, 2048, 2055, 3234, 3813
Mva1269I GAATGC 3 cut(s) 865, 1040, 1804
MvaI CCWGG 6 cut(s) 388, 928, 1613, 2538, 2787, 2824
MvnI CGCG 1 cut(s) 2806
NciI CCSGG 1 cut(s) 972
NcoI CCATGG 3 cut(s) 1467, 2515, 3918
NdeI CATATG 1 cut(s) 3298
NlaIV GGNNCC 7 cut(s) 1813, 1952, 2521, 2895, 3351, 3810, 4008
NmeAIII GCCGAG 1 cut(s) 2893
NmuCI GTSAC 2 cut(s) 2739, 3701
NsbI TGCGCA 1 cut(s) 2288
NsiI ATGCAT 4 cut(s) 319, 865, 1267, 2284
NspI RCATGY 5 cut(s) 521, 1073, 1157, 1256, 4089
OliI CACNNNNGTG 1 cut(s) 1545
PaeR7I CTCGAG 1 cut(s) 845
PagI TCATGA 2 cut(s) 1009, 3061
PceI AGGCCT 1 cut(s) 2332
PciSI GCTCTTC 1 cut(s) 3180
PcsI WCGNNNNNNNCGW 1 cut(s) 2803
PctI GAATGC 3 cut(s) 865, 1040, 1804
PdmI GAANNNNTTC 3 cut(s) 201, 294, 2461
PflFI GACNNNGTC 1 cut(s) 1972
PfoI TCCNGGA 1 cut(s) 1611
PleI GAGTC 5 cut(s) 68, 1281, 1981, 2242, 2471
PmaCI CACGTG 1 cut(s) 3751
PmlI CACGTG 1 cut(s) 3751
PpsI GAGTC 5 cut(s) 68, 1281, 1981, 2242, 2471
Ppu21I YACGTR 1 cut(s) 3751
PshBI ATTAAT 2 cut(s) 285, 2934
PsiI TTATAA 3 cut(s) 1410, 2930, 3170
Psp124BI GAGCTC 2 cut(s) 93, 606
Psp6I CCWGG 6 cut(s) 386, 926, 1611, 2536, 2785, 2822
PspCI CACGTG 1 cut(s) 3751
PspFI CCCAGC 1 cut(s) 4032
PspGI CCWGG 6 cut(s) 386, 926, 1611, 2536, 2785, 2822
PspN4I GGNNCC 7 cut(s) 1813, 1952, 2521, 2895, 3351, 3810, 4008
PspPI GGNCC 6 cut(s) 505, 2068, 2519, 2708, 3532, 3650
PsrI GAACNNNNNNTAC 2 cut(s) 4075, 4107
PstI CTGCAG 1 cut(s) 2203
PstNI CAGNNNCTG 2 cut(s) 371, 1084
PsuI RGATCY 3 cut(s) 420, 3158, 3271
PsyI GACNNNGTC 1 cut(s) 1972
PvuII CAGCTG 2 cut(s) 1207, 4036
RsaI GTAC 7 cut(s) 250, 976, 1293, 2413, 3002, 3071, 4093
RsaNI GTAC 7 cut(s) 249, 975, 1292, 2412, 3001, 3070, 4092
RseI CAYNNNNRTG 5 cut(s) 754, 1478, 1545, 1809, 2514
SacI GAGCTC 2 cut(s) 93, 606
SapI GCTCTTC 1 cut(s) 3180
Sau96I GGNCC 6 cut(s) 505, 2068, 2519, 2708, 3532, 3650
ScaI AGTACT 2 cut(s) 2413, 3002
SchI GAGTC 5 cut(s) 68, 1282, 1982, 2242, 2471
ScrFI CCNGG 7 cut(s) 388, 928, 972, 1613, 2538, 2787, 2824
SduI GDGCHC 5 cut(s) 93, 606, 2752, 2761, 3262
SexAI ACCWGGT 1 cut(s) 2822
SfaNI GCATC 9 cut(s) 52, 601, 658, 725, 1210, 1525, 2269, 2909, 3596
SfcI CTRYAG 4 cut(s) 137, 2199, 2712, 3873
Sfr274I CTCGAG 1 cut(s) 845
SinI GGWCC 5 cut(s) 2068, 2519, 2708, 3532, 3650
SlaI CTCGAG 1 cut(s) 845
SmiMI CAYNNNNRTG 5 cut(s) 754, 1478, 1545, 1809, 2514
SmlI CTYRAG 7 cut(s) 116, 299, 845, 1343, 1682, 3253, 3527
SmoI CTYRAG 7 cut(s) 116, 299, 845, 1343, 1682, 3253, 3527
SseBI AGGCCT 1 cut(s) 2332
SsiI CCGC 9 cut(s) 24, 496, 651, 884, 1107, 2297, 2369, 2407, 4039
SspI AATATT 7 cut(s) 934, 1825, 2845, 2941, 3739, 3942, 4063
SspMI CTAG 5 cut(s) 380, 1304, 1566, 1890, 3986
SstI GAGCTC 2 cut(s) 93, 606
StuI AGGCCT 1 cut(s) 2332
StyD4I CCNGG 7 cut(s) 386, 926, 970, 1611, 2536, 2785, 2822
StyI CCWWGG 9 cut(s) 1467, 1946, 2333, 2515, 3008, 3099, 3389, 3487, 3918
TaaI ACNGT 9 cut(s) 253, 569, 742, 1296, 1364, 1441, 2115, 2639, 3021
TaiI ACGT 1 cut(s) 3753
TaqI TCGA 6 cut(s) 846, 1940, 2352, 2651, 3468, 4075
TaqII GACCGA 1 cut(s) 1347
TatI WGTACW 5 cut(s) 248, 1291, 2411, 3000, 4091
TauI GCSGC 1 cut(s) 2371
TscAI CASTG 9 cut(s) 258, 761, 796, 1230, 1367, 1843, 2761, 3778, 3871
TseFI GTSAC 2 cut(s) 2739, 3701
Tsp45I GTSAC 2 cut(s) 2739, 3701
TspGWI ACGGA 2 cut(s) 67, 596
TspRI CASTG 9 cut(s) 258, 761, 796, 1230, 1367, 1843, 2761, 3778, 3871
Tth111I GACNNNGTC 1 cut(s) 1972
Vha464I CTTAAG 1 cut(s) 3253
VneI GTGCAC 1 cut(s) 2748
VpaK11BI GGWCC 5 cut(s) 2068, 2519, 2708, 3532, 3650
VspI ATTAAT 2 cut(s) 285, 2934
XagI CCTNNNNNAGG 1 cut(s) 2805
XceI RCATGY 5 cut(s) 521, 1073, 1157, 1256, 4089
XcmI CCANNNNNNNNNTGG 1 cut(s) 3387
XhoI CTCGAG 1 cut(s) 845
XmiI GTMKAC 2 cut(s) 1300, 1677
XmnI GAANNNNTTC 3 cut(s) 201, 294, 2461
XspI CTAG 5 cut(s) 380, 1304, 1566, 1890, 3986
ZrmI AGTACT 2 cut(s) 2413, 3002
Zsp2I ATGCAT 4 cut(s) 319, 865, 1267, 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.