pycom07g11320
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012661
Physical Location & Seq
Reverse (-)
11976787 .. 11978091
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGCTTCTTAGAAGCTTTGAAGATGAAACCGTTCAAAGAGTGGCAGCCTTTGCCATTGCGAATCTTGCAATGAATGAAGCTAATCAAGAAATTATACTGGAGCAAGGTGGGATTAGTTTGTTAGCAACGACAGCTGCTGATGCTGATGATGCTCAAACCCTACGCATGATTGCTGGAGCTATTGCTAATTTATGCGGCAATGATAAGCTACAAATGAAGCTAAGGTTCGAAGGTGGTATCAAGGCGTTGCTAGGAATTGTGAGGTGTGGGCATCCTGATGTTCTTTCCCAAGTGGTGCGTGGAATTGCTAACTTTGCAAAATGCGAGTCACGAGCATGTACTCAAGGGATAAGGAATGGAAGATCTTTCTTGATAGAAGATGGAGCACTTCCATGGATTGTACAAAATGCTAATGACGAGGCTGCACCAATCCGGCGCCACATTGAGCTTGCACTCTATCACTTAGCACAACATGAGGTGAATGCAAAGGACATGATTAGTGGAGGTGCCCTCTGGGAGCTAGTTCGTATCTCCTGA

Protein Analysis

179

Amino Acids

19.32

Weight (kDa)

5.56

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 2 - 159 1.5e-12 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 435, 506
AciI CCGC 1 cut(s) 195
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 2 cut(s) 340, 402
AgsI TTSAA 2 cut(s) 20, 35
AluBI AGCT 8 cut(s) 15, 80, 134, 179, 208, 220, 448, 520
AluI AGCT 8 cut(s) 15, 80, 134, 179, 208, 220, 448, 520
Alw21I GWGCWC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 137
ApeKI GCWGC 3 cut(s) 44, 134, 422
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
Asp700I GAANNNNTTC 1 cut(s) 30
AspLEI GCGC 1 cut(s) 438
AsuHPI GGTGA 1 cut(s) 490
AsuII TTCGAA 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 511
BanI GGYRCC 2 cut(s) 435, 506
BauI CACGAG 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 56, 121, 409
BccI CCATC 1 cut(s) 374
BfaI CTAG 2 cut(s) 251, 521
BfoI RGCGCY 1 cut(s) 439
BglII AGATCT 1 cut(s) 362
BisI GCNGC 4 cut(s) 45, 135, 196, 423
BlsI GCNGC 4 cut(s) 46, 136, 197, 424
BmiI GGNNCC 2 cut(s) 437, 508
BmsI GCATC 3 cut(s) 130, 139, 280
BplI GAGNNNNNCTC 2 cut(s) 495, 527
BpmI CTGGAG 2 cut(s) 119, 195
Bpu10I CCTNAGC 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 3 cut(s) 54, 75, 205
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 271
BseMI GCAATG 3 cut(s) 54, 75, 205
BseNI ACTGG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 3 cut(s) 56, 121, 409
BsgI GTGCAG 1 cut(s) 408
BshNI GGYRCC 2 cut(s) 435, 506
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 433
BsmI GAATGC 1 cut(s) 487
Bsp119I TTCGAA 1 cut(s) 228
Bsp1286I GDGCHC 2 cut(s) 388, 511
Bsp1407I TGTACA 1 cut(s) 400
Bsp143I GATC 1 cut(s) 362
Bsp19I CCATGG 1 cut(s) 392
BspACI CCGC 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 437, 508
BspT104I TTCGAA 1 cut(s) 228
BspT107I GGYRCC 2 cut(s) 435, 506
BsrDI GCAATG 3 cut(s) 54, 75, 205
BsrGI TGTACA 1 cut(s) 400
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 1 cut(s) 362
BssNI GRCGYC 1 cut(s) 436
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 31
BstACI GRCGYC 1 cut(s) 436
BstAPI GCANNNNNTGC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 400
BstBI TTCGAA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 3 cut(s) 8, 221, 463
BstDSI CCRYGG 1 cut(s) 392
BstF5I GGATG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 1 cut(s) 365
BstMBI GATC 1 cut(s) 362
BstMWI GCNNNNNNNGC 6 cut(s) 50, 65, 131, 140, 149, 314
BstNSI RCATGY 1 cut(s) 339
BstSLI GKGCMC 1 cut(s) 511
BstV1I GCAGC 3 cut(s) 56, 121, 409
BstX2I RGATCY 1 cut(s) 362
BstYI RGATCY 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 392
BtsCI GGATG 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 450
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 438
Csp6I GTAC 2 cut(s) 339, 401
CviAII CATG 5 cut(s) 166, 336, 393, 473, 493
CviQI GTAC 2 cut(s) 339, 401
DdeI CTNAG 3 cut(s) 8, 221, 463
DinI GGCGCC 1 cut(s) 437
DpnI GATC 1 cut(s) 364
DpnII GATC 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 392
EcoT14I CCWWGG 1 cut(s) 392
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 392
FaeI CATG 5 cut(s) 169, 339, 396, 476, 496
FaiI YATR 7 cut(s) 95, 167, 193, 337, 394, 474, 494
FatI CATG 5 cut(s) 165, 335, 392, 472, 492
Fnu4HI GCNGC 4 cut(s) 45, 135, 196, 423
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 45, 135, 196, 423
FspBI CTAG 2 cut(s) 251, 521
GlaI GCGC 1 cut(s) 437
GluI GCNGC 4 cut(s) 45, 135, 196, 423
GsuI CTGGAG 2 cut(s) 119, 195
HaeII RGCGCY 1 cut(s) 439
HapII CCGG 1 cut(s) 433
HhaI GCGC 1 cut(s) 438
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 5 cut(s) 169, 339, 396, 476, 496
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 2 cut(s) 61, 326
HpaII CCGG 1 cut(s) 433
HphI GGTGA 1 cut(s) 490
Hpy188III TCNNGA 5 cut(s) 86, 275, 330, 370, 534
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 5 cut(s) 68, 317, 425, 452, 485
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 65, 131, 140, 149, 314
HpyF3I CTNAG 3 cut(s) 8, 221, 463
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 5 cut(s) 169, 339, 396, 476, 496
HspAI GCGC 1 cut(s) 436
KasI GGCGCC 1 cut(s) 435
Kzo9I GATC 1 cut(s) 362
LmnI GCTCC 4 cut(s) 100, 176, 383, 517
LpnPI CCDG 5 cut(s) 83, 159, 288, 446, 499
Lsp1109I GCAGC 3 cut(s) 56, 121, 409
LweI GCATC 3 cut(s) 130, 139, 280
MaeI CTAG 2 cut(s) 251, 521
MaeIII GTNAC 1 cut(s) 327
MalI GATC 1 cut(s) 364
MboI GATC 1 cut(s) 362
MboII GAAGA 3 cut(s) 32, 372, 389
MflI RGATCY 1 cut(s) 362
MhlI GDGCHC 2 cut(s) 388, 511
MluCI AATT 4 cut(s) 90, 187, 255, 303
Mly113I GGCGCC 1 cut(s) 436
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 5 cut(s) 255, 412, 469, 497, 521
MroXI GAANNNNTTC 1 cut(s) 30
MslI CAYNNNNRTG 3 cut(s) 276, 334, 391
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 487
MwoI GCNNNNNNNGC 6 cut(s) 50, 65, 131, 140, 149, 314
NarI GGCGCC 1 cut(s) 436
NcoI CCATGG 1 cut(s) 392
NdeII GATC 1 cut(s) 362
NlaIII CATG 5 cut(s) 169, 339, 396, 476, 496
NlaIV GGNNCC 2 cut(s) 437, 508
NmuCI GTSAC 1 cut(s) 327
NspI RCATGY 1 cut(s) 339
NspV TTCGAA 1 cut(s) 228
PctI GAATGC 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 4 cut(s) 46, 136, 197, 424
PleI GAGTC 1 cut(s) 334
PluTI GGCGCC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 437, 508
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 2 cut(s) 340, 402
RsaNI GTAC 2 cut(s) 339, 401
RseI CAYNNNNRTG 3 cut(s) 276, 334, 391
SatI GCNGC 4 cut(s) 45, 135, 196, 423
Sau3AI GATC 1 cut(s) 362
SchI GAGTC 1 cut(s) 335
SduI GDGCHC 2 cut(s) 388, 511
SfaNI GCATC 3 cut(s) 130, 139, 280
SfoI GGCGCC 1 cut(s) 437
SfuI TTCGAA 1 cut(s) 228
SmiMI CAYNNNNRTG 3 cut(s) 276, 334, 391
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 4 cut(s) 90, 187, 255, 303
SsiI CCGC 1 cut(s) 195
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 2 cut(s) 251, 521
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 228
TasI AATT 4 cut(s) 90, 187, 255, 303
TatI WGTACW 2 cut(s) 338, 400
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 1 cut(s) 61
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 3 cut(s) 44, 134, 422
Tsp45I GTSAC 1 cut(s) 327
TspDTI ATGAA 4 cut(s) 39, 86, 90, 230
XceI RCATGY 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 2 cut(s) 251, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.