Rorug07G0061500
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012661
Physical Location & Seq
Forward (+)
4480192 .. 4481681
1490 bp
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UTR
Exon/CDS
Intron
Rorug07G0061500.1

Sequence Viewer

Length: 822 bp
ATGTTACGCATAAGCAACACCGTGATCGGGATCCTCAATGCGATGTTCCTCCTCCTGGGACTCGCCTCCATCGGCGCGTCCATCTACTTCCACCTCCACGGCGGCACAGTCTGCCAGAAGGTCCTCCAGAACCCTCTCCTCTTCGCCGGACTCTTCTTCGTTGTCGTGTCCCTCCTCGGCCTCGCCGGATCGTGCTGCCGACTCAACTCTCTCCTCTACACCTACCTCGTCTTAATCTTTCTCCTCATCCTCGGACTCATCGCGTTCACCGTGTTCGCGCTTTTCGTGACCAACAAGGGGATCGGACAGGCCGTGTCGGGACGAGGGTACAAGGAGTACAAGGTTGGAGACTTCTCGCATTGGCTCCAGCATTACGTTGTTAATGGGAAGAACTGGGAGCCGATCAAGAGTTGTCTCATTGATGCTCAGGTTTGCCAGAGTCTCGGCGGCGATGCTCATCAGACCCAGTCCGATTTTTACAAGAAGAACTTGTCTCCGATTCAGTCCGGTTGCTGCAAGCCACCATCCTACTGCGGATATGAATTCAAGAATGCAACATATTGGGTTGTTCCAAAGTCGGGACCTAAAGAGCCGGACAGTGATTGCATGACTTGGAGCAACAAGCAGAACAAGTTGTGTTATGACTGCAAGTCATGCAAAGGAGGAATGCTGGCGAACATTCGCAAAGAGTGGAGGTCTCTGGCCATACTCAACTGCTGTATTCTCGTTCTCCTTACCCTCGTCTACTGCGTTGGCTGCTGTGCGACCAAGAACAATCGCTTCAGTACTTACAATTACAACAAGTACAGAGGAGGCGCTTGA

Protein Analysis

273

Amino Acids

30.25

Weight (kDa)

9.08

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 6 - 255 1.5e-31 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 744
AccII CGCG 3 cut(s) 77, 263, 278
AciI CCGC 3 cut(s) 102, 447, 534
AclWI GGATC 4 cut(s) 25, 38, 196, 308
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 766
AfaI GTAC 4 cut(s) 329, 338, 787, 806
AfiI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 578
AgsI TTSAA 1 cut(s) 547
AhdI GACNNNNNGTC 1 cut(s) 649
AjnI CCWGG 1 cut(s) 54
Alw26I GTCTC 5 cut(s) 342, 419, 446, 498, 702
AlwI GGATC 4 cut(s) 25, 38, 196, 308
AoxI GGCC 3 cut(s) 178, 309, 702
ApeKI GCWGC 3 cut(s) 195, 513, 756
ApoI RAATTY 1 cut(s) 542
AspLEI GCGC 3 cut(s) 77, 280, 818
AspS9I GGNCC 2 cut(s) 121, 581
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 2 cut(s) 121, 581
BalI TGGCCA 1 cut(s) 704
BamHI GGATCC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 182, 500, 743
BccI CCATC 3 cut(s) 77, 89, 532
BceAI ACGGC 2 cut(s) 115, 296
BciT130I CCWGG 1 cut(s) 56
BcoDI GTCTC 5 cut(s) 342, 419, 446, 498, 702
BfoI RGCGCY 1 cut(s) 819
BisI GCNGC 5 cut(s) 103, 196, 448, 514, 757
BlsI GCNGC 5 cut(s) 104, 197, 449, 515, 758
BmcAI AGTACT 1 cut(s) 787
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 2 cut(s) 121, 581
BmeRI GACNNNNNGTC 1 cut(s) 649
BmgT120I GGNCC 2 cut(s) 121, 581
BmiI GGNNCC 4 cut(s) 32, 365, 399, 582
BmrFI CCNGG 1 cut(s) 56
BmrI ACTGGG 2 cut(s) 403, 460
BmsI GCATC 2 cut(s) 412, 442
BmuI ACTGGG 2 cut(s) 403, 460
BpmI CTGGAG 2 cut(s) 110, 350
Bpu10I CCTNAGC 1 cut(s) 426
BsaBI GATNNNNATC 2 cut(s) 29, 456
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 55, 97, 175, 250
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 55, 297, 578
Bse1I ACTGG 2 cut(s) 398, 466
Bse8I GATNNNNATC 2 cut(s) 29, 456
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 4 cut(s) 55, 97, 175, 250
BseGI GGATG 2 cut(s) 246, 524
BseJI GATNNNNATC 2 cut(s) 29, 456
BseLI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 578
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 398, 466
BseRI GAGGAG 5 cut(s) 41, 128, 164, 203, 233
BseXI GCAGC 3 cut(s) 182, 500, 743
Bsh1236I CGCG 3 cut(s) 77, 263, 278
BshFI GGCC 3 cut(s) 180, 311, 704
BsiSI CCGG 4 cut(s) 147, 186, 507, 593
BslFI GGGAC 4 cut(s) 72, 154, 333, 594
BslI CCNNNNNNNGG 4 cut(s) 27, 55, 297, 578
BsmAI GTCTC 5 cut(s) 342, 419, 446, 498, 702
BsmFI GGGAC 4 cut(s) 72, 154, 333, 594
BsmI GAATGC 2 cut(s) 556, 672
BsnI GGCC 3 cut(s) 180, 311, 704
Bso31I GGTCTC 1 cut(s) 702
Bsp143I GATC 5 cut(s) 24, 30, 188, 300, 402
BspACI CCGC 3 cut(s) 102, 447, 534
BspANI GGCC 3 cut(s) 180, 311, 704
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 3 cut(s) 77, 263, 278
BspLI GGNNCC 4 cut(s) 32, 365, 399, 582
BspPI GGATC 4 cut(s) 25, 38, 196, 308
BspTNI GGTCTC 1 cut(s) 702
BsrI ACTGG 2 cut(s) 398, 466
BssECI CCNNGG 4 cut(s) 55, 97, 175, 250
BssMI GATC 5 cut(s) 24, 30, 188, 300, 402
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 4 cut(s) 22, 109, 271, 599
Bst6I CTCTTC 2 cut(s) 146, 158
BstAPI GCANNNNNTGC 2 cut(s) 111, 654
BstC8I GCNNGC 2 cut(s) 518, 672
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 246, 524
BstFNI CGCG 3 cut(s) 77, 263, 278
BstH2I RGCGCY 1 cut(s) 819
BstHHI GCGC 3 cut(s) 77, 280, 818
BstKTI GATC 5 cut(s) 27, 33, 191, 303, 405
BstMAI GTCTC 5 cut(s) 342, 419, 446, 498, 702
BstMBI GATC 5 cut(s) 24, 30, 188, 300, 402
BstMWI GCNNNNNNNGC 3 cut(s) 111, 654, 756
BstNI CCWGG 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 54
BstUI CGCG 3 cut(s) 77, 263, 278
BstV1I GCAGC 3 cut(s) 182, 500, 743
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 3 cut(s) 180, 311, 704
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 3 cut(s) 56, 244, 465
BtsCI GGATG 2 cut(s) 246, 524
BtsIMutI CAGTG 1 cut(s) 604
Cac8I GCNNGC 2 cut(s) 518, 672
CfoI GCGC 3 cut(s) 77, 280, 818
Cfr13I GGNCC 2 cut(s) 121, 581
CseI GACGC 1 cut(s) 66
Csp6I GTAC 4 cut(s) 328, 337, 786, 805
CviAII CATG 2 cut(s) 607, 654
CviJI RGCY 8 cut(s) 180, 311, 364, 400, 520, 592, 704, 756
CviKI_1 RGCY 8 cut(s) 180, 311, 364, 400, 520, 592, 704, 756
CviQI GTAC 4 cut(s) 328, 337, 786, 805
DdeI CTNAG 1 cut(s) 426
DpnI GATC 5 cut(s) 26, 32, 190, 302, 404
DpnII GATC 5 cut(s) 24, 30, 188, 300, 402
DriI GACNNNNNGTC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 2 cut(s) 146, 158
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 2 cut(s) 146, 158
Eco31I GGTCTC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 121, 581
Eco57I CTGAAG 1 cut(s) 766
EcoO109I RGGNCCY 2 cut(s) 121, 581
EcoRI GAATTC 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 610, 657
FaiI YATR 7 cut(s) 11, 540, 559, 608, 642, 655, 707
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 4 cut(s) 72, 154, 333, 594
FatI CATG 2 cut(s) 606, 653
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 5 cut(s) 103, 196, 448, 514, 757
FokI GGATG 2 cut(s) 233, 511
Fsp4HI GCNGC 5 cut(s) 103, 196, 448, 514, 757
GlaI GCGC 3 cut(s) 76, 279, 817
GluI GCNGC 5 cut(s) 103, 196, 448, 514, 757
GsuI CTGGAG 2 cut(s) 110, 350
HaeII RGCGCY 1 cut(s) 819
HaeIII GGCC 3 cut(s) 180, 311, 704
HapII CCGG 4 cut(s) 147, 186, 507, 593
HgaI GACGC 1 cut(s) 66
HhaI GCGC 3 cut(s) 77, 280, 818
Hin1II CATG 2 cut(s) 610, 657
Hin6I GCGC 3 cut(s) 75, 278, 816
HinP1I GCGC 3 cut(s) 75, 278, 816
HinfI GANTC 6 cut(s) 60, 150, 201, 255, 439, 499
HpaII CCGG 4 cut(s) 147, 186, 507, 593
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 267, 745
Hpy188I TCNGA 5 cut(s) 254, 305, 462, 472, 498
Hpy188III TCNNGA 7 cut(s) 28, 127, 286, 318, 406, 547, 579
Hpy8I GTNNAC 2 cut(s) 267, 745
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 4 cut(s) 22, 109, 271, 599
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 516, 554, 606, 648, 657
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 654, 756
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 2 cut(s) 610, 657
HspAI GCGC 3 cut(s) 75, 278, 816
Kzo9I GATC 5 cut(s) 24, 30, 188, 300, 402
LmnI GCTCC 3 cut(s) 369, 397, 615
Lsp1109I GCAGC 3 cut(s) 182, 500, 743
LweI GCATC 2 cut(s) 412, 442
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 2 cut(s) 3, 286
MalI GATC 5 cut(s) 26, 32, 190, 302, 404
MboI GATC 5 cut(s) 24, 30, 188, 300, 402
MboII GAAGA 5 cut(s) 133, 145, 148, 400, 496
MflI RGATCY 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 704
MluCI AATT 2 cut(s) 542, 793
MluNI TGGCCA 1 cut(s) 704
MlyI GAGTC 5 cut(s) 54, 144, 195, 249, 448
MmeI TCCRAC 1 cut(s) 325
Mox20I TGGCCA 1 cut(s) 704
MscI TGGCCA 1 cut(s) 704
MseI TTAA 2 cut(s) 233, 381
Msp20I TGGCCA 1 cut(s) 704
MspI CCGG 4 cut(s) 147, 186, 507, 593
MspR9I CCNGG 1 cut(s) 56
Mva1269I GAATGC 2 cut(s) 556, 672
MvaI CCWGG 1 cut(s) 56
MvnI CGCG 3 cut(s) 77, 263, 278
MwoI GCNNNNNNNGC 3 cut(s) 111, 654, 756
NdeII GATC 5 cut(s) 24, 30, 188, 300, 402
NlaIII CATG 2 cut(s) 610, 657
NlaIV GGNNCC 4 cut(s) 32, 365, 399, 582
NmeAIII GCCGAG 2 cut(s) 156, 423
NmuCI GTSAC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 4 cut(s) 267, 282, 309, 747
PctI GAATGC 2 cut(s) 556, 672
PfeI GAWTC 1 cut(s) 499
PflFI GACNNNGTC 1 cut(s) 466
PkrI GCNGC 5 cut(s) 104, 197, 449, 515, 758
PleI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpsI GAGTC 5 cut(s) 54, 144, 195, 249, 447
PpuMI RGGWCCY 2 cut(s) 121, 581
Psp5II RGGWCCY 2 cut(s) 121, 581
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 32, 365, 399, 582
PspPI GGNCC 2 cut(s) 121, 581
PspPPI RGGWCCY 2 cut(s) 121, 581
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 466
RsaI GTAC 4 cut(s) 329, 338, 787, 806
RsaNI GTAC 4 cut(s) 328, 337, 786, 805
SaqAI TTAA 2 cut(s) 233, 381
SatI GCNGC 5 cut(s) 103, 196, 448, 514, 757
Sau3AI GATC 5 cut(s) 24, 30, 188, 300, 402
Sau96I GGNCC 2 cut(s) 121, 581
ScaI AGTACT 1 cut(s) 787
SchI GAGTC 5 cut(s) 54, 144, 195, 249, 448
ScrFI CCNGG 1 cut(s) 56
SetI ASST 9 cut(s) 96, 123, 224, 228, 345, 378, 432, 586, 698
SfaNI GCATC 2 cut(s) 412, 442
SinI GGWCC 2 cut(s) 121, 581
Sse9I AATT 2 cut(s) 542, 793
SsiI CCGC 3 cut(s) 102, 447, 534
StyD4I CCNGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 22, 109, 271, 599
TaiI ACGT 1 cut(s) 378
TasI AATT 2 cut(s) 542, 793
TatI WGTACW 3 cut(s) 336, 785, 804
TauI GCSGC 2 cut(s) 105, 450
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 2 cut(s) 233, 381
Tru9I TTAA 2 cut(s) 233, 381
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 195, 513, 756
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 555
TspRI CASTG 1 cut(s) 604
Tth111I GACNNNGTC 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 121, 581
XapI RAATTY 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.