pycom07g13550

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006678
Physical Location & Seq
Forward (+)
15439950 .. 15443018
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGGCACAATTCTCAGCTCCTCAAGTATCCAGTACGCTGAGGGCGCCACGCAGGCCAATTGCTTGTTGCAAGGCGACGACAATGGCTTGGTTGTCGTCCTGCACACAGACTGCGGTAGCATCCCTTAAGCTCACTCCGGGGTTTCGGGGCTTGCCGGAGCTCAATTCCTGTCGTTTGAGAGTATTGGGGAGTGATCGGTCTCGGAGTTTCGCCATGGCAAGAGACAGTGCTGAGGGGACTGCGGTGGGTGATCCTGTGGAAAATGCTGGTCGCAATCAGGCTGAAGATTCTTCAACAGAAAAGTTTGAGGAGCGTGATTTTACTGGCACGCCGTATGTTCCTGTGTATGTGATGTTACCGTTGGGTGTGATCAATATGAATGTTGAGCTAGTTGAGCCACAAGCTCTGCGAAATCAACTACAAGTGTTGAAGTCGGTTGGTGTTGATGGTGTTATGGTTGATTGCTGGTGGGGAATAGTAGAGGCGCACAATCCACAGCAATATAATTGGAATGGCTACAAGAGACTTTTTCAGATTGTGCGTGATCTTAACCTTAAGCTACAGGTCGTGATGTCATTCCATGAATGTGGAGGCAATGTCGGGGATGATGTACATATCCCACTTCCTCACTGGGTGACAGAAATTGGCCAAAAGAATCCTGATATATATTTTACAAATAAAGAAGGGAAACGCAATGCTGAATGTCTCACATGGGGAATTGATAAGGAGCGAGTTTTAAGAGGCCGTACTGCTGTTGAGGTTTACTTCGACTACATGAGGAGCTTCAGAGTTGAATTTAATGAATTTTTTGAGGAAGGAATAATATCAGAAATTGACGTTGGATTAGGTCCGTGTGGAGAGCTACGGTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGACCGATACTTGATGAAGGATCGATGGCACAGGCTGCAGAAGCAAGGGGCCACTCCTTTTGGGCCAGAGCACCAGATAATGCAGGTTCTTATAGTTCCCAACCACATGAGACAGGGTTTTTCCGTGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTCCTCAATTGGTACTCCCGTGTTTTGA

Protein Analysis

373

Amino Acids

42.06

Weight (kDa)

5.96

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 116 - 314 2.2e-79 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1003
AccB1I GGYRCC 1 cut(s) 43
AciI CCGC 2 cut(s) 113, 242
AclWI GGATC 2 cut(s) 245, 957
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 3 cut(s) 794, 803, 917
AcuI CTGAAG 2 cut(s) 303, 769
AcyI GRCGYC 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 634
AfaI GTAC 4 cut(s) 34, 612, 748, 1105
AfiI CCNNNNNNNGG 1 cut(s) 911
AflII CTTAAG 2 cut(s) 125, 554
AgsI TTSAA 3 cut(s) 294, 430, 794
AjnI CCWGG 1 cut(s) 904
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AluBI AGCT 9 cut(s) 17, 130, 160, 388, 404, 559, 783, 862, 1076
AluI AGCT 9 cut(s) 17, 130, 160, 388, 404, 559, 783, 862, 1076
Alw21I GWGCWC 2 cut(s) 162, 1002
Alw26I GTCTC 5 cut(s) 204, 216, 517, 710, 1033
AlwI GGATC 2 cut(s) 245, 957
AoxI GGCC 5 cut(s) 53, 646, 742, 978, 992
ApeKI GCWGC 1 cut(s) 964
ApoI RAATTY 3 cut(s) 794, 803, 917
AspLEI GCGC 2 cut(s) 46, 487
AspS9I GGNCC 3 cut(s) 848, 978, 992
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 4 cut(s) 260, 646, 926, 1075
AvaII GGWCC 1 cut(s) 848
BaeI ACNNNNGTAYC 1 cut(s) 1095
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 162, 1002
BbvCI CCTCAGC 2 cut(s) 38, 231
BbvI GCAGC 1 cut(s) 951
BccI CCATC 2 cut(s) 440, 948
BceAI ACGGC 2 cut(s) 316, 729
BciT130I CCWGG 1 cut(s) 906
BciVI GTATCC 2 cut(s) 37, 879
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 5 cut(s) 204, 216, 517, 710, 1033
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 2 cut(s) 560, 965
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 2 cut(s) 125, 554
BfuAI ACCTGC 1 cut(s) 1003
BfuI GTATCC 2 cut(s) 37, 879
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 1 cut(s) 965
BlsI GCNGC 1 cut(s) 966
Bme1390I CCNGG 2 cut(s) 138, 906
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 3 cut(s) 848, 978, 992
BmiI GGNNCC 2 cut(s) 45, 979
BmrFI CCNGG 2 cut(s) 138, 906
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 640
Bpu10I CCTNAGC 2 cut(s) 38, 231
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 138
Bsa29I ATCGAT 1 cut(s) 952
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 137, 213
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 911
Bse1I ACTGG 4 cut(s) 30, 328, 635, 922
Bse3DI GCAATG 2 cut(s) 601, 700
BseBI CCWGG 1 cut(s) 906
BseCI ATCGAT 1 cut(s) 952
BseDI CCNNGG 2 cut(s) 137, 213
BseGI GGATG 2 cut(s) 119, 610
BseLI CCNNNNNNNGG 1 cut(s) 911
BseMI GCAATG 2 cut(s) 601, 700
BseMII CTCAG 3 cut(s) 27, 29, 222
BseNI ACTGG 4 cut(s) 30, 328, 635, 922
BseRI GAGGAG 3 cut(s) 9, 323, 793
BseXI GCAGC 1 cut(s) 951
BsgI GTGCAG 1 cut(s) 85
BshFI GGCC 5 cut(s) 55, 648, 744, 980, 994
BshNI GGYRCC 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 952
BsiHKAI GWGCWC 2 cut(s) 162, 1002
BsiSI CCGG 2 cut(s) 137, 155
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 1 cut(s) 911
BsmAI GTCTC 5 cut(s) 204, 216, 517, 710, 1033
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 5 cut(s) 55, 648, 744, 980, 994
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 162, 1002
Bsp1407I TGTACA 1 cut(s) 610
Bsp143I GATC 5 cut(s) 193, 250, 369, 544, 949
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 2 cut(s) 113, 242
BspANI GGCC 5 cut(s) 55, 648, 744, 980, 994
BspCNI CTCAG 3 cut(s) 26, 30, 223
BspDI ATCGAT 1 cut(s) 952
BspLI GGNNCC 2 cut(s) 45, 979
BspMAI CTGCAG 1 cut(s) 969
BspMI ACCTGC 1 cut(s) 1003
BspPI GGATC 2 cut(s) 245, 957
BspT107I GGYRCC 1 cut(s) 43
BspTI CTTAAG 2 cut(s) 125, 554
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 601, 700
BsrGI TGTACA 1 cut(s) 610
BsrI ACTGG 4 cut(s) 30, 328, 635, 922
BssECI CCNNGG 2 cut(s) 137, 213
BssMI GATC 5 cut(s) 193, 250, 369, 544, 949
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 906
Bst4CI ACNGT 3 cut(s) 227, 360, 867
BstACI GRCGYC 1 cut(s) 44
BstAFI CTTAAG 2 cut(s) 125, 554
BstAPI GCANNNNNTGC 1 cut(s) 964
BstAUI TGTACA 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 53, 152, 329
BstDEI CTNAG 3 cut(s) 13, 38, 231
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 119, 610
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 2 cut(s) 46, 487
BstKTI GATC 5 cut(s) 196, 253, 372, 547, 952
BstMAI GTCTC 5 cut(s) 204, 216, 517, 710, 1033
BstMBI GATC 5 cut(s) 193, 250, 369, 544, 949
BstMWI GCNNNNNNNGC 6 cut(s) 43, 52, 394, 964, 970, 1082
BstNI CCWGG 1 cut(s) 906
BstSCI CCNGG 2 cut(s) 136, 904
BstSFI CTRYAG 2 cut(s) 560, 965
BstV1I GCAGC 1 cut(s) 951
BstXI CCANNNNNNTGG 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 952
BsuI GTATCC 2 cut(s) 37, 879
BsuRI GGCC 5 cut(s) 55, 648, 744, 980, 994
BsuTUI ATCGAT 1 cut(s) 952
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 2 cut(s) 119, 610
BtsIMutI CAGTG 3 cut(s) 232, 628, 929
BveI ACCTGC 1 cut(s) 1003
Cac8I GCNNGC 3 cut(s) 53, 152, 329
CfoI GCGC 2 cut(s) 46, 487
Cfr13I GGNCC 3 cut(s) 848, 978, 992
ClaI ATCGAT 1 cut(s) 952
Csp6I GTAC 4 cut(s) 33, 611, 747, 1104
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviAII CATG 6 cut(s) 214, 581, 711, 775, 890, 1036
CviQI GTAC 4 cut(s) 33, 611, 747, 1104
DdeI CTNAG 3 cut(s) 13, 38, 231
DinI GGCGCC 1 cut(s) 45
DpnI GATC 5 cut(s) 195, 252, 371, 546, 951
DpnII GATC 5 cut(s) 193, 250, 369, 544, 949
DraIII CACNNNGTG 1 cut(s) 634
EaeI YGGCCR 1 cut(s) 646
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 303, 769
EcoICRI GAGCTC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 917
EcoRII CCWGG 1 cut(s) 904
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 6 cut(s) 217, 584, 714, 778, 893, 1039
FaqI GGGAC 1 cut(s) 250
FatI CATG 6 cut(s) 213, 580, 710, 774, 889, 1035
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 1 cut(s) 965
FokI GGATG 2 cut(s) 106, 617
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 965
FspBI CTAG 1 cut(s) 389
GlaI GCGC 2 cut(s) 45, 486
GluI GCNGC 1 cut(s) 965
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 5 cut(s) 55, 648, 744, 980, 994
HapII CCGG 2 cut(s) 137, 155
HhaI GCGC 2 cut(s) 46, 487
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 6 cut(s) 217, 584, 714, 778, 893, 1039
Hin6I GCGC 2 cut(s) 44, 485
HinP1I GCGC 2 cut(s) 44, 485
HinfI GANTC 3 cut(s) 287, 655, 1088
HpaII CCGG 2 cut(s) 137, 155
HphI GGTGA 4 cut(s) 260, 646, 926, 1075
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 4 cut(s) 204, 534, 788, 829
Hpy188III TCNNGA 3 cut(s) 568, 659, 881
Hpy8I GTNNAC 1 cut(s) 763
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 4 cut(s) 677, 809, 882, 940
HpyCH4III ACNGT 3 cut(s) 227, 360, 867
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 4 cut(s) 69, 102, 967, 1012
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 52, 394, 964, 970, 1082
HpyF3I CTNAG 3 cut(s) 13, 38, 231
HpySE526I ACGT 1 cut(s) 837
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 6 cut(s) 217, 584, 714, 778, 893, 1039
HspAI GCGC 2 cut(s) 44, 485
KasI GGCGCC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 5 cut(s) 193, 250, 369, 544, 949
LmnI GCTCC 5 cut(s) 22, 157, 310, 727, 780
Lsp1109I GCAGC 1 cut(s) 951
LweI GCATC 1 cut(s) 128
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 837
MaeIII GTNAC 3 cut(s) 354, 634, 1054
MalI GATC 5 cut(s) 195, 252, 371, 546, 951
MboI GATC 5 cut(s) 193, 250, 369, 544, 949
MboII GAAGA 3 cut(s) 282, 296, 1083
MfeI CAATTG 2 cut(s) 57, 1098
MhlI GDGCHC 2 cut(s) 162, 1002
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
Mly113I GGCGCC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 820, 875
Mox20I TGGCCA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 648
MseI TTAA 5 cut(s) 126, 549, 555, 737, 798
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 1 cut(s) 648
MspCI CTTAAG 2 cut(s) 125, 554
MspI CCGG 2 cut(s) 137, 155
MspR9I CCNGG 2 cut(s) 138, 906
MunI CAATTG 2 cut(s) 57, 1098
MvaI CCWGG 1 cut(s) 906
MwoI GCNNNNNNNGC 6 cut(s) 43, 52, 394, 964, 970, 1082
NarI GGCGCC 1 cut(s) 44
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 213
NdeII GATC 5 cut(s) 193, 250, 369, 544, 949
NlaIII CATG 6 cut(s) 217, 584, 714, 778, 893, 1039
NlaIV GGNNCC 2 cut(s) 45, 979
NmuCI GTSAC 2 cut(s) 634, 1054
PfeI GAWTC 3 cut(s) 287, 655, 1088
PkrI GCNGC 1 cut(s) 966
PluTI GGCGCC 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 904
PspGI CCWGG 1 cut(s) 904
PspN4I GGNNCC 2 cut(s) 45, 979
PspPI GGNCC 3 cut(s) 848, 978, 992
PstI CTGCAG 1 cut(s) 969
RsaI GTAC 4 cut(s) 34, 612, 748, 1105
RsaNI GTAC 4 cut(s) 33, 611, 747, 1104
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 5 cut(s) 126, 549, 555, 737, 798
SatI GCNGC 1 cut(s) 965
Sau3AI GATC 5 cut(s) 193, 250, 369, 544, 949
Sau96I GGNCC 3 cut(s) 848, 978, 992
ScrFI CCNGG 2 cut(s) 138, 906
SduI GDGCHC 2 cut(s) 162, 1002
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 560, 965
SfoI GGCGCC 1 cut(s) 45
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 3 cut(s) 21, 125, 554
SmoI CTYRAG 3 cut(s) 21, 125, 554
SsiI CCGC 2 cut(s) 113, 242
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 1 cut(s) 389
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 136, 904
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 3 cut(s) 227, 360, 867
TaiI ACGT 1 cut(s) 840
TaqI TCGA 2 cut(s) 768, 952
TaqII GACCGA 2 cut(s) 186, 948
TatI WGTACW 1 cut(s) 610
TfiI GAWTC 3 cut(s) 287, 655, 1088
Tru1I TTAA 5 cut(s) 126, 549, 555, 737, 798
Tru9I TTAA 5 cut(s) 126, 549, 555, 737, 798
TscAI CASTG 3 cut(s) 232, 635, 929
TseFI GTSAC 2 cut(s) 634, 1054
TseI GCWGC 1 cut(s) 964
Tsp45I GTSAC 2 cut(s) 634, 1054
TspDTI ATGAA 4 cut(s) 392, 597, 816, 959
TspGWI ACGGA 3 cut(s) 840, 1042, 1073
TspRI CASTG 3 cut(s) 232, 635, 929
Vha464I CTTAAG 2 cut(s) 125, 554
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 3 cut(s) 794, 803, 917
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.