Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0010310

Physical Location & Seq

Genomic Coordinates Reverse (-)

18276045 .. 18281479

Gene Structure 5435 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3501 bp

ATGGAGGAGGCTAGGGTTTCAGGGGACACTGGGAATGGTGCTCCCAGTGAGGGGGCTGTTCGGCCCTCGTACTGGTTGGATGCTTGTGAGGACATACCGTGTGATGTAATTGGTGATTTCGTGGAGTTCTGTGAACCTCCAGTTTGTGGTCCTCCTCCTGTGGTGGCGGCGGCCAATGGCAATGGCTGCAGCCAGGAGGAGGATGGGTTGGTGGGCGATTTTTTCGGGGGGATTGATCACTTTCTCGACAGTTTCAAGAGTGGAGCTGGCCTCCCTGGTGTGACTGATCCGAACTTGGATGTGAATGCCAATGGTGTTATTGGCAATGCTGCTGTTGAGGGTTGTGCTAAGATTGAGCCACCTGTGGTTGTGCAGTGGGGGAATGTTGATGACACGGTTGGACACAATGTTTGGAGTGGAGGGAGGAATTTGGAGGGTGATAATGGAGAGAGTAAGGATGATCGGAGTGGTTATCACAGGTATGAGAAGAGGAAGGGCAATGGGGTTGTGAGGAAGAGAGATATGGATGGTGAGGAGAGGTTCTTTAAGAGGGTTGCTTTGGATAATGGTAGGAATGAGAGGTATAGTTCCGGTAGAGGGCAATATAACATGAGGGACAAAAGTTTTAGTAGGAAGAGGCCTCGTGATTCTGAGGATATTGATTGGAGGGATAGAGATAGAGATAGAGATCGGATTAGGAGGAGAAGAGAGAATTATGGTAGTTATAATAGGAGGGAGGTTGCTAGAGATAGGGAGGCAAAGGGTTATTGGGAGAGGGATAAGTTGGGAACCAATGAGCTTGTTTTCCGATTAGGAACTTACAAACCTGATCATAACAAAGAGGGTAGGATAGCCGATGTCAAAAACAAGGAATGCAATGGGAAGGCTGACAAGAAACCCGAAGAGGTTAAAGAAAAACCTCCCGAGGAACAAGTCAGAAAGTATCAATTGGATGTGCTCGAACAGGCAAAGAAGAGAAATACAATAGCTTTTCTTGAAACTGGGGCCGGAAAGACACTCATTGCTGTTCTCCTCATGCAAAGTGTTTGCAATGATATGCAGCAAAAGCAGAACAAAAAGATGCTTTCTGTATTTCTCGTTCCTAAAGTTCCCCTTGTTTATCAGCAAGCAGAAGTTATTCGTGAGCGAACTGGTTTCCAAGTGGGCCATTATTGTGGTGAGATGGGCCAAGATTTCTGGGACGCCCGAAGATGGCATCATGAATTCGACACAAAACAGGTTCTGTTAATGACAGCTCAAATTCTGTTGAACATTCTGAGGCACAGCATCATAAAAATGGAAGCAATCAATCTCTTGATTCTGGATGAGTGTCATCACGCTGTGAAGAAACATCCATATTCTTTAGTGATGTCTGAGTTCTATCATACAACCCCAAAGGAGAAAAGGCCGTCTGTATTTGGAATGACTGCTTCTCCTGTTAATTTAAAAGGTGTTTCCAGCCAAATAGATTGTGCAATAAAGATTCGTAATCTTGAAAGTAAACTAGATTCCATAGTTTGTACCATAAAGGACCGCAAGGAGCTTGAAAAGCATGTTCCAATGCCCTCCGAAACAGTGGTGCAGTATGATAAAGGAGCTAGCTTATGGTCCCTTCATGAGCAGCTAAAAAAAATGGAAGTGGAAGTTGAAGAAGCTGCAAAGTTCAGCTCTAGGAAAAGTAAATGGCAGTTTATGGGAGCCAGAGATGCTGGGGCTAAGGAAGAGCTGCGCCAAATGTATGGTGTTTCCGAAAGAACAGAAAGTGATGGGGCTGTTAACTTAACTCAAAAGTTGAGGGCTATTAATTATGCGCTCGGTGAACTGGGTCAGTGGTGTGCTTACAAGGTTGCACAATCTTTCTTGACAGCTTTACAGAATGACGAGAGGGTGAGCTACCAGCTTGATGTCAGGTTTCAGGAATCCTACCTGAGCAAAGTTGTAAATCTCTTACAATGTCAACTATCAGAGGGAGCTGTTTTGGACAAGGAAAAGAAAGTAGCAGATTCAGAGAATGGTGTTTCTTGTGATGGAAATGATCCTGATGAGATGGAGGAAGGAGAGCTACTTGATACTCATGTTGCCTCTGGTGGGGAGCATGTAGATGTGATAATAGGAGCTGCTGTAGCTGATGGGAAGGTGACTCCAAAAGTGCAGTCACTGGTAAAGGTACTTCTCAAGTATCAGCATACAAAAGATTTTCGCGCAATCATCTTTGTGGAGCGAGTTGTGTCTGCTTTAGTCCTTCCCAAGGTCTTAAAGGAACTTCCTTCTTTAGGTTTTATAGAGTGTGCTAGCCTTATTGGCCACAACAATAGTCAGGAATTGAGAAGCTGCCAAATGCAGGACACTATTGCCAAATTCAAGGATGGCCGTGTGACATTGTTAGTTGCAACTAGTGTTGCCGAGGAAGGACTTGATATTCGGCAGTGCAATGTTGTCATTCGGTTTGACCTTGCAAAAACTGTTTTGGCTTACATACAGTCCAGGGGTCGTGCTAGAAAACCCGGGTCTGATTACATCTTGATGGTGGAGAGAGGAAATCTGGCACATGAAGCATTCTTAAGGAATGCCAGGAAAAGCGAGGAGACTTTACAGAAGGAAGCAATAGAGAGAACTGACCTGAGTCATCTGAAGGATAGTGCTAGGTTAATTTCAGCGGATACAACGCCAGAGACAGTGTACCAGGTGGAGTCAACTGGTGCTGTTGTGAGCTTAAATTCTGCTGTTGGACTCACCCATTTCTACTGCTCTCAGCTTCCCAGCGACAGGTACTCAATTCTTCGTCCTGAGTTTGTTATGGTGAGGCATGAGAAGCAAGGAGGTCACACTGAATATTCATGCAAACTTCAACTCCCATGTAACGCACCATTTGAGGCACTTGAGGGTCCTCTGTCCAGTTCAATGCACCTTGCACGGCAGGCTGTTTGCTTGGCTGCTTGCAAGAAACTCCATGAGATGGGAGCATTTACCGACATGCTCTTGCCAGACAAGGGAGTTGGGGAAGAAAAAGAGCAGGTTGACCAGAATGATGGCGGAGATCGACTTCCTGGAACTGCTAGGCACAGAGAGTTCTATCCCGAAGGTGTAGCTAATATCCTCAAGGGAGAATGGATCTTATCTGGAAGAGATTTGGGTCGTGACTTGGAATTAATTCATGTTTACATGTATGGCGTGAAATATGTAAACTCTGGCTCTTCAAAAGATCCTTTCTTGACTCAAGTTTCGGATTTTGCAGTTCTAGTAGGAAATGAACTGGATGCAGAGGTGTTATCGATGTCGGTGGATCTATTTATCGCTCGAACCATGACTATAGATGCATCCCTTGTTTTCAGGGGTTCAATATATATTACTGAAAGTCAGTTGGTGTCCCTTAAAAGTTTTCATGTAAGATTAATGAGTATTGTACTGGACGTGGATGTCGAACCTTCCACCACCCCTTGGGACCATGCAAAGGCTTATTTATTTGTCCCTGTGGTTAGCGATAAGTTGGTGGATCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004525 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016442 GO:0016458 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031332 GO:0032296 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

DEAD DND1_DSRM Dicer_dimer Helicase_C PAZ ResIII Ribonuclease_3 dsrm

Protein Analysis

1167

Amino Acids

130.72

Weight (kDa)

5.94

Isoelectric Point (pI)

39.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	312 - 479	1.6e-14	Type III restriction enzyme, res subunit
DEAD	PF00270	314 - 479	2.5e-15	DEAD/DEAH box helicase
Helicase_C	PF00271	716 - 833	3.9e-16	Helicase conserved C-terminal domain
Dicer_dimer	PF03368	908 - 996	2.1e-20	Dicer dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01040 AT1G01040
fragaria_vesca	FvH4_7g17920 FvH4_7g17920 FvH4_7g17920 FvH4_7g17920
malus_domestica	MD01G1091600.v1.1 MD07G1161800.v1.1
prunus_persica	Prupe.2G200900_v2.0.a1
pyrus_communis	pycom01g11560 pycom07g15740
rosa_chinensis	RchiOBHm_Chr1g0361361
rosa_laevigata	RLG00000027722
rosa_multiflora	Rmu_sc0002119.1_g000026
rosa_roxburghii	Rroxscaffold_4G00295020
rosa_rugosa	Rorug01G0288900
rosa_samantha	Rh1AG299700 Rh1BG263500 Rh1CG281100 Rh1DG293500
rosa_wichuraiana	Rw1G026540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	726
AasI	GACNNNNNNGTC	1 cut(s)	3416
Acc36I	ACCTGC	1 cut(s)	3004
AccB7I	CCANNNNNTGG	3 cut(s)	146, 2956, 3438
AccII	CGCG	1 cut(s)	2202
AciI	CCGC	5 cut(s)	167, 170, 1534, 2657, 3033
AclWI	GGATC	5 cut(s)	281, 2030, 3119, 3197, 3291
AcoI	YGGCCR	3 cut(s)	171, 2302, 2368
AcsI	RAATTY	5 cut(s)	427, 1223, 1260, 2357, 2716
AcuI	CTGAAG	1 cut(s)	2651
AcyI	GRCGYC	1 cut(s)	1203
AdeI	CACNNNGTG	1 cut(s)	1342
AfaI	GTAC	6 cut(s)	71, 1522, 2169, 2681, 2771, 3405
AflII	CTTAAG	1 cut(s)	2560
AflIII	ACRYGT	1 cut(s)	3162
AhlI	ACTAGT	1 cut(s)	2393
AjiI	CACGTC	1 cut(s)	3412
AjnI	CCWGG	6 cut(s)	192, 274, 2483, 2570, 2682, 3046
Alw21I	GWGCWC	2 cut(s)	43, 960
Alw26I	GTCTC	2 cut(s)	2579, 2666
AlwI	GGATC	5 cut(s)	281, 2030, 3119, 3197, 3291
AlwNI	CAGNNNCTG	2 cut(s)	1243, 2158
Ama87I	CYCGRG	2 cut(s)	923, 2504
ApoI	RAATTY	5 cut(s)	427, 1223, 1260, 2357, 2716
ArsI	GACNNNNNNTTYG	8 cut(s)	393, 425, 2138, 2170, 2403, 2435, 3205, 3237
AseI	ATTAAT	3 cut(s)	1803, 3149, 3392
Asp700I	GAANNNNTTC	2 cut(s)	1137, 3150
AspLEI	GCGC	3 cut(s)	1731, 1813, 2204
AspS9I	GGNCC	9 cut(s)	63, 149, 1005, 1165, 1186, 1531, 1608, 2885, 3442
AsuC2I	CCSGG	2 cut(s)	2505, 2506
AsuHPI	GGTGA	9 cut(s)	125, 449, 542, 1190, 1829, 1900, 2149, 2725, 2812
AsuNHI	GCTAGC	2 cut(s)	1598, 2291
AvaI	CYCGRG	2 cut(s)	923, 2504
AvaII	GGWCC	5 cut(s)	149, 1531, 1608, 2885, 3442
BaeI	ACNNNNGTAYC	2 cut(s)	2061, 2094
BalI	TGGCCA	1 cut(s)	2304
BarI	GAAGNNNNNNTAC	4 cut(s)	2046, 2078, 2154, 2186
BauI	CACGAG	1 cut(s)	642
Bbv12I	GWGCWC	2 cut(s)	43, 960
BceAI	ACGGC	3 cut(s)	1393, 2355, 2930
BciT130I	CCWGG	6 cut(s)	194, 276, 2485, 2572, 2684, 3048
BciVI	GTATCC	1 cut(s)	2653
BclI	TGATCA	2 cut(s)	235, 829
BcnI	CCSGG	2 cut(s)	2505, 2506
BcoDI	GTCTC	2 cut(s)	2579, 2666
BcuI	ACTAGT	1 cut(s)	2393
BfmI	CTRYAG	3 cut(s)	187, 2121, 3309
BfrI	CTTAAG	1 cut(s)	2560
BfuAI	ACCTGC	1 cut(s)	3004
BfuI	GTATCC	1 cut(s)	2653
BglI	GCCNNNNNGGC	1 cut(s)	2301
Bme1390I	CCNGG	8 cut(s)	194, 276, 2485, 2505, 2506, 2572, 2684, 3048
Bme18I	GGWCC	5 cut(s)	149, 1531, 1608, 2885, 3442
BmeT110I	CYCGRG	2 cut(s)	923, 2504
BmgBI	CACGTC	1 cut(s)	3412
BmgT120I	GGNCC	9 cut(s)	63, 149, 1005, 1165, 1186, 1531, 1608, 2885, 3442
BmiI	GGNNCC	6 cut(s)	790, 1006, 1610, 1699, 2886, 3443
BmrFI	CCNGG	8 cut(s)	194, 276, 2485, 2505, 2506, 2572, 2684, 3048
BmrI	ACTGGG	4 cut(s)	39, 39, 1011, 1832
BmsI	GCATC	8 cut(s)	70, 1071, 1225, 1296, 1696, 3247, 3304, 3326
BmtI	GCTAGC	2 cut(s)	1602, 2295
BmuI	ACTGGG	4 cut(s)	39, 39, 1011, 1832
BoxI	GACNNNNGTC	1 cut(s)	2622
BplI	GAGNNNNNCTC	2 cut(s)	255, 287
BpmI	CTGGAG	1 cut(s)	123
Bpu10I	CCTNAGC	2 cut(s)	1716, 1928
BpuEI	CTTGAG	4 cut(s)	2159, 2900, 3083, 3201
BpuMI	CCSGG	2 cut(s)	2505, 2506
Bsa29I	ATCGAT	2 cut(s)	3272, 3496
BsaBI	GATNNNNATC	1 cut(s)	687
BsaHI	GRCGYC	1 cut(s)	1203
BsaJI	CCNNGG	7 cut(s)	274, 924, 2247, 2403, 2484, 2504, 3437
BsaWI	WCCGGW	1 cut(s)	590
BsaXI	ACNNNNNCTCC	8 cut(s)	191, 221, 1417, 1447, 2106, 2136, 2835, 2865
Bse3DI	GCAATG	7 cut(s)	187, 331, 505, 883, 1020, 1057, 2437
Bse8I	GATNNNNATC	1 cut(s)	687
BseBI	CCWGG	6 cut(s)	194, 276, 2485, 2572, 2684, 3048
BseCI	ATCGAT	2 cut(s)	3272, 3496
BseDI	CCNNGG	7 cut(s)	274, 924, 2247, 2403, 2484, 2504, 3437
BseJI	GATNNNNATC	1 cut(s)	687
BseMI	GCAATG	7 cut(s)	187, 331, 505, 883, 1020, 1057, 2437
BseMII	CTCAG	7 cut(s)	642, 1268, 1365, 1919, 2612, 2765, 2778
BseRI	GAGGAG	7 cut(s)	20, 144, 212, 548, 715, 1022, 2597
BseYI	CCCAGC	2 cut(s)	1709, 2759
BsgI	GTGCAG	3 cut(s)	392, 1601, 2171
Bsh1236I	CGCG	1 cut(s)	2202
BshVI	ATCGAT	2 cut(s)	3272, 3496
BsiHKAI	GWGCWC	2 cut(s)	43, 960
BsiHKCI	CYCGRG	2 cut(s)	923, 2504
BsiSI	CCGG	3 cut(s)	591, 1008, 2505
BslFI	GGGAC	7 cut(s)	38, 629, 1214, 1594, 3352, 3452, 3455
BsmAI	GTCTC	2 cut(s)	2579, 2666
BsmFI	GGGAC	7 cut(s)	38, 629, 1214, 1594, 3352, 3452, 3455
BsmI	GAATGC	4 cut(s)	310, 878, 2555, 2572
BsoBI	CYCGRG	2 cut(s)	923, 2504
Bsp1286I	GDGCHC	2 cut(s)	43, 960
BspACI	CCGC	5 cut(s)	167, 170, 1534, 2657, 3033
BspCNI	CTCAG	7 cut(s)	643, 1269, 1366, 1920, 2613, 2764, 2779
BspDI	ATCGAT	2 cut(s)	3272, 3496
BspFNI	CGCG	1 cut(s)	2202
BspHI	TCATGA	2 cut(s)	1219, 1615
BspLI	GGNNCC	6 cut(s)	790, 1006, 1610, 1699, 2886, 3443
BspMAI	CTGCAG	1 cut(s)	191
BspMI	ACCTGC	1 cut(s)	3004
BspOI	GCTAGC	2 cut(s)	1602, 2295
BspPI	GGATC	5 cut(s)	281, 2030, 3119, 3197, 3291
BspQI	GCTCTTC	2 cut(s)	1716, 3199
BspTI	CTTAAG	1 cut(s)	2560
BsrDI	GCAATG	7 cut(s)	187, 331, 505, 883, 1020, 1057, 2437
BssECI	CCNNGG	7 cut(s)	274, 924, 2247, 2403, 2484, 2504, 3437
BssNI	GRCGYC	1 cut(s)	1203
BssSI	CACGAG	1 cut(s)	642
BssT1I	CCWWGG	2 cut(s)	2247, 3437
Bst2BI	CACGAG	1 cut(s)	642
Bst2UI	CCWGG	6 cut(s)	194, 276, 2485, 2572, 2684, 3048
Bst4CI	ACNGT	7 cut(s)	99, 251, 397, 1576, 2464, 2481, 2677
Bst6I	CTCTTC	9 cut(s)	482, 509, 629, 700, 897, 968, 1716, 3118, 3199
BstACI	GRCGYC	1 cut(s)	1203
BstAFI	CTTAAG	1 cut(s)	2560
BstAPI	GCANNNNNTGC	1 cut(s)	186
BstC8I	GCNNGC	6 cut(s)	268, 1128, 1600, 2293, 2919, 2938
BstDEI	CTNAG	9 cut(s)	348, 651, 1277, 1374, 1716, 1928, 2621, 2751, 2787
BstENI	CCTNNNNNAGG	2 cut(s)	2246, 2271
BstFNI	CGCG	1 cut(s)	2202
BstHHI	GCGC	3 cut(s)	1731, 1813, 2204
BstMAI	GTCTC	2 cut(s)	2579, 2666
BstMWI	GCNNNNNNNGC	9 cut(s)	186, 1066, 1549, 1706, 2123, 2301, 2552, 2812, 2918
BstNI	CCWGG	6 cut(s)	194, 276, 2485, 2572, 2684, 3048
BstNSI	RCATGY	4 cut(s)	1556, 2099, 2977, 3166
BstPAI	GACNNNNGTC	1 cut(s)	2622
BstSCI	CCNGG	8 cut(s)	192, 274, 2483, 2503, 2504, 2570, 2682, 3046
BstSFI	CTRYAG	3 cut(s)	187, 2121, 3309
BstUI	CGCG	1 cut(s)	2202
BstX2I	RGATCY	3 cut(s)	3111, 3202, 3283
BstXI	CCANNNNNNTGG	3 cut(s)	1175, 1739, 3029
BstYI	RGATCY	3 cut(s)	3111, 3202, 3283
Bsu15I	ATCGAT	2 cut(s)	3272, 3496
BsuI	GTATCC	1 cut(s)	2653
BsuTUI	ATCGAT	2 cut(s)	3272, 3496
BtrI	CACGTC	1 cut(s)	3412
BtsI	GCAGTG	2 cut(s)	380, 2432
BtsIMutI	CAGTG	9 cut(s)	27, 52, 380, 1581, 1835, 2156, 2432, 2682, 2826
BveI	ACCTGC	1 cut(s)	3004
Cac8I	GCNNGC	6 cut(s)	268, 1128, 1600, 2293, 2919, 2938
CaiI	CAGNNNCTG	2 cut(s)	1243, 2158
CciI	TCATGA	2 cut(s)	1219, 1615
CfoI	GCGC	3 cut(s)	1731, 1813, 2204
Cfr13I	GGNCC	9 cut(s)	63, 149, 1005, 1165, 1186, 1531, 1608, 2885, 3442
Cfr9I	CCCGGG	1 cut(s)	2504
ClaI	ATCGAT	2 cut(s)	3272, 3496
CseI	GACGC	1 cut(s)	1211
CsiI	ACCWGGT	1 cut(s)	2682
Csp6I	GTAC	6 cut(s)	70, 1521, 2168, 2680, 2770, 3404
CviQI	GTAC	6 cut(s)	70, 1521, 2168, 2680, 2770, 3404
DdeI	CTNAG	9 cut(s)	348, 651, 1277, 1374, 1716, 1928, 2621, 2751, 2787
DraI	TTTAAA	1 cut(s)	1446
DraIII	CACNNNGTG	1 cut(s)	1342
DrdI	GACNNNNNNGTC	1 cut(s)	3416
DseDI	GACNNNNNNGTC	1 cut(s)	3416
EaeI	YGGCCR	3 cut(s)	171, 2302, 2368
Eam1104I	CTCTTC	9 cut(s)	482, 509, 629, 700, 897, 968, 1716, 3118, 3199
EarI	CTCTTC	9 cut(s)	482, 509, 629, 700, 897, 968, 1716, 3118, 3199
EciI	GGCGGA	1 cut(s)	3048
Eco130I	CCWWGG	2 cut(s)	2247, 3437
Eco147I	AGGCCT	1 cut(s)	640
Eco47I	GGWCC	5 cut(s)	149, 1531, 1608, 2885, 3442
Eco57I	CTGAAG	1 cut(s)	2651
Eco88I	CYCGRG	2 cut(s)	923, 2504
EcoNI	CCTNNNNNAGG	2 cut(s)	2246, 2271
EcoO109I	RGGNCCY	1 cut(s)	2885
EcoRI	GAATTC	1 cut(s)	1223
EcoRII	CCWGG	6 cut(s)	192, 274, 2483, 2570, 2682, 3046
EcoT14I	CCWWGG	2 cut(s)	2247, 3437
EcoT22I	ATGCAT	1 cut(s)	3319
ErhI	CCWWGG	2 cut(s)	2247, 3437
FalI	AAGNNNNNCTT	2 cut(s)	1098, 1130
FaqI	GGGAC	7 cut(s)	38, 629, 1214, 1594, 3352, 3452, 3455
FbaI	TGATCA	2 cut(s)	235, 829
GlaI	GCGC	3 cut(s)	1730, 1812, 2203
GsaI	CCCAGC	2 cut(s)	1713, 2763
GsuI	CTGGAG	1 cut(s)	123
HapII	CCGG	3 cut(s)	591, 1008, 2505
HgaI	GACGC	1 cut(s)	1211
HhaI	GCGC	3 cut(s)	1731, 1813, 2204
Hin1I	GRCGYC	1 cut(s)	1203
Hin6I	GCGC	3 cut(s)	1729, 1811, 2202
HinP1I	GCGC	3 cut(s)	1729, 1811, 2202
HincII	GTYRAC	4 cut(s)	1777, 1958, 2694, 3019
HindII	GTYRAC	4 cut(s)	1777, 1958, 2694, 3019
HpaI	GTTAAC	1 cut(s)	1777
HpaII	CCGG	3 cut(s)	591, 1008, 2505
HphI	GGTGA	9 cut(s)	125, 449, 542, 1190, 1829, 1900, 2149, 2725, 2812
HpyCH4III	ACNGT	7 cut(s)	99, 251, 397, 1576, 2464, 2481, 2677
HpyCH4IV	ACGT	1 cut(s)	3411
HpyF10VI	GCNNNNNNNGC	9 cut(s)	186, 1066, 1549, 1706, 2123, 2301, 2552, 2812, 2918
HpyF3I	CTNAG	9 cut(s)	348, 651, 1277, 1374, 1716, 1928, 2621, 2751, 2787
HpySE526I	ACGT	1 cut(s)	3411
Hsp92I	GRCGYC	1 cut(s)	1203
HspAI	GCGC	3 cut(s)	1729, 1811, 2202
Ksp22I	TGATCA	2 cut(s)	235, 829
KspAI	GTTAAC	1 cut(s)	1777
LguI	GCTCTTC	2 cut(s)	1716, 3199
LweI	GCATC	8 cut(s)	70, 1071, 1225, 1296, 1696, 3247, 3304, 3326
MabI	ACCWGGT	1 cut(s)	2682
MaeII	ACGT	1 cut(s)	3411
MaeIII	GTNAC	7 cut(s)	280, 2137, 2154, 2374, 2822, 2858, 3137
MfeI	CAATTG	1 cut(s)	947
MflI	RGATCY	3 cut(s)	3111, 3202, 3283
MhlI	GDGCHC	2 cut(s)	43, 960
MlsI	TGGCCA	1 cut(s)	2304
MluNI	TGGCCA	1 cut(s)	2304
MlyI	GAGTC	5 cut(s)	2134, 2632, 2699, 2724, 3208
MmeI	TCCRAC	3 cut(s)	57, 379, 2707
Mox20I	TGGCCA	1 cut(s)	2304
Mph1103I	ATGCAT	1 cut(s)	3319
MroXI	GAANNNNTTC	2 cut(s)	1137, 3150
MscI	TGGCCA	1 cut(s)	2304
MslI	CAYNNNNRTG	6 cut(s)	480, 1173, 1295, 2100, 2213, 2522
Msp20I	TGGCCA	1 cut(s)	2304
MspA1I	CMGCKG	1 cut(s)	2657
MspCI	CTTAAG	1 cut(s)	2560
MspI	CCGG	3 cut(s)	591, 1008, 2505
MspR9I	CCNGG	8 cut(s)	194, 276, 2485, 2505, 2506, 2572, 2684, 3048
MunI	CAATTG	1 cut(s)	947
Mva1269I	GAATGC	4 cut(s)	310, 878, 2555, 2572
MvaI	CCWGG	6 cut(s)	194, 276, 2485, 2572, 2684, 3048
MvnI	CGCG	1 cut(s)	2202
MwoI	GCNNNNNNNGC	9 cut(s)	186, 1066, 1549, 1706, 2123, 2301, 2552, 2812, 2918
NciI	CCSGG	2 cut(s)	2505, 2506
NheI	GCTAGC	2 cut(s)	1598, 2291
NlaIV	GGNNCC	6 cut(s)	790, 1006, 1610, 1699, 2886, 3443
NmeAIII	GCCGAG	1 cut(s)	2428
NmuCI	GTSAC	6 cut(s)	280, 2137, 2154, 2374, 2822, 3137
NsiI	ATGCAT	1 cut(s)	3319
NspI	RCATGY	4 cut(s)	1556, 2099, 2977, 3166
PagI	TCATGA	2 cut(s)	1219, 1615
PceI	AGGCCT	1 cut(s)	640
PciI	ACATGT	1 cut(s)	3162
PciSI	GCTCTTC	2 cut(s)	1716, 3199
PcsI	WCGNNNNNNNCGW	1 cut(s)	3417
PctI	GAATGC	4 cut(s)	310, 878, 2555, 2572
PdmI	GAANNNNTTC	2 cut(s)	1137, 3150
PfeI	GAWTC	6 cut(s)	647, 1318, 1483, 1508, 1919, 2003
PflMI	CCANNNNNTGG	3 cut(s)	146, 2956, 3438
PfoI	TCCNGGA	1 cut(s)	3046
PleI	GAGTC	5 cut(s)	2134, 2631, 2698, 2724, 3208
PpsI	GAGTC	5 cut(s)	2134, 2631, 2698, 2724, 3208
PpuMI	RGGWCCY	1 cut(s)	2885
PscI	ACATGT	1 cut(s)	3162
PshAI	GACNNNNGTC	1 cut(s)	2622
PshBI	ATTAAT	3 cut(s)	1803, 3149, 3392
PsiI	TTATAA	1 cut(s)	726
Psp5II	RGGWCCY	1 cut(s)	2885
Psp6I	CCWGG	6 cut(s)	192, 274, 2483, 2570, 2682, 3046
PspFI	CCCAGC	2 cut(s)	1709, 2759
PspGI	CCWGG	6 cut(s)	192, 274, 2483, 2570, 2682, 3046
PspN4I	GGNNCC	6 cut(s)	790, 1006, 1610, 1699, 2886, 3443
PspPI	GGNCC	9 cut(s)	63, 149, 1005, 1165, 1186, 1531, 1608, 2885, 3442
PspPPI	RGGWCCY	1 cut(s)	2885
PsrI	GAACNNNNNNTAC	2 cut(s)	1083, 1115
PstI	CTGCAG	1 cut(s)	191
PstNI	CAGNNNCTG	2 cut(s)	1243, 2158
PsuI	RGATCY	3 cut(s)	3111, 3202, 3283
RsaI	GTAC	6 cut(s)	71, 1522, 2169, 2681, 2771, 3405
RsaNI	GTAC	6 cut(s)	70, 1521, 2168, 2680, 2770, 3404
RseI	CAYNNNNRTG	6 cut(s)	480, 1173, 1295, 2100, 2213, 2522
SapI	GCTCTTC	2 cut(s)	1716, 3199
Sau96I	GGNCC	9 cut(s)	63, 149, 1005, 1165, 1186, 1531, 1608, 2885, 3442
SchI	GAGTC	5 cut(s)	2134, 2632, 2699, 2724, 3208
ScrFI	CCNGG	8 cut(s)	194, 276, 2485, 2505, 2506, 2572, 2684, 3048
SduI	GDGCHC	2 cut(s)	43, 960
SexAI	ACCWGGT	1 cut(s)	2682
SfaNI	GCATC	8 cut(s)	70, 1071, 1225, 1296, 1696, 3247, 3304, 3326
SfcI	CTRYAG	3 cut(s)	187, 2121, 3309
SinI	GGWCC	5 cut(s)	149, 1531, 1608, 2885, 3442
SmaI	CCCGGG	1 cut(s)	2506
SmiMI	CAYNNNNRTG	6 cut(s)	480, 1173, 1295, 2100, 2213, 2522
SmlI	CTYRAG	5 cut(s)	2174, 2560, 2879, 3098, 3216
SmoI	CTYRAG	5 cut(s)	2174, 2560, 2879, 3098, 3216
SpeI	ACTAGT	1 cut(s)	2393
SseBI	AGGCCT	1 cut(s)	640
SsiI	CCGC	5 cut(s)	167, 170, 1534, 2657, 3033
SspI	AATATT	1 cut(s)	2834
StuI	AGGCCT	1 cut(s)	640
StyD4I	CCNGG	8 cut(s)	192, 274, 2483, 2503, 2504, 2570, 2682, 3046
StyI	CCWWGG	2 cut(s)	2247, 3437
TaaI	ACNGT	7 cut(s)	99, 251, 397, 1576, 2464, 2481, 2677
TaiI	ACGT	1 cut(s)	3414
TaqI	TCGA	8 cut(s)	246, 960, 1227, 3040, 3272, 3298, 3420, 3496
TatI	WGTACW	1 cut(s)	3403
TauI	GCSGC	2 cut(s)	170, 173
TfiI	GAWTC	6 cut(s)	647, 1318, 1483, 1508, 1919, 2003
TscAI	CASTG	9 cut(s)	34, 52, 380, 1581, 1835, 2163, 2432, 2682, 2833
TseFI	GTSAC	6 cut(s)	280, 2137, 2154, 2374, 2822, 3137
Tsp45I	GTSAC	6 cut(s)	280, 2137, 2154, 2374, 2822, 3137
TspDTI	ATGAA	7 cut(s)	1236, 1604, 2565, 2826, 3143, 3264, 3371
TspMI	CCCGGG	1 cut(s)	2504
TspRI	CASTG	9 cut(s)	34, 52, 380, 1581, 1835, 2163, 2432, 2682, 2833
Van91I	CCANNNNNTGG	3 cut(s)	146, 2956, 3438
Vha464I	CTTAAG	1 cut(s)	2560
VpaK11BI	GGWCC	5 cut(s)	149, 1531, 1608, 2885, 3442
VspI	ATTAAT	3 cut(s)	1803, 3149, 3392
XagI	CCTNNNNNAGG	2 cut(s)	2246, 2271
XapI	RAATTY	5 cut(s)	427, 1223, 1260, 2357, 2716
XceI	RCATGY	4 cut(s)	1556, 2099, 2977, 3166
XcmI	CCANNNNNNNNNTGG	1 cut(s)	200
XmaI	CCCGGG	1 cut(s)	2504
XmnI	GAANNNNTTC	2 cut(s)	1137, 3150
Zsp2I	ATGCAT	1 cut(s)	3319

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.