pycom07g20650

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003416
Physical Location & Seq
Reverse (-)
22667548 .. 22670381
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2631 bp
ATGAATTCTCTCCACCGAATACAAACAGATCTCAGTCCTCCCAATCCCACCAACTTCTTTTTCTCTTGTTCCATTCCCTCCCTCCCTCTCTTATCACTCTCACTTTCTCTCTCTAGAATCAAGCATAAACAGTTCGCCATTGTTGGTGCTCTGTTTCTGACCATGGACAGCAAAGGTCAGAACTTTCCCGCATTTTCCGACTACCCATCTCAAAATTGGAGCCGGATTTCCCACAAGAAGAAGGAAAAATGGAAGTTTCTTCCTCTCCAACCCAAACGCGCCCACTTCCTCCTCTTTCTCCACCTCCTCCTCTCCTTCTTCCACCCGGCCAGGCCCGAAATCTGGGACGGCGTCATTGTCACGGCGGCGGACTACCAATCCCTCCAAGCAATCAAGCACGAGCTCGACGACCCAAAGGGGTTCTTAAGGAGCTGGAACGACAGCGGTTACGGAGCCTGCTCCGGCGGATGGGCCGGAATCAAGTGCGCTCAGGGGCAGGTGATTGTGCTCCAGCTTCCCTGGAGGGGGTTAGGTGGCAGAATCTCCGAGAAAATCGGACTGCTCCAGGCGCTGAGGAAGCTGAGCCTCCATGACAACCAGATTGAAGGGCCAATCCCTCAATCTTTAGGGTACCTTCCAAGCCTCAGAGGAGTTCAATTATTTAACAATAAGCTTTCGGGTTCAATCCCAATTTCTTTAGGGTTTAGTCCTCTGCTCCAAACCCTTGATCTTAGCAACAATTTGCTCATTGATAAAATCCCAGGCAGCATTTTTAACTCCACCAAACTTTATAGGCTCAATCTGAGCTTCAATTCGCTTTCGGGTTCTATCCCGGGTAGTATCTCCCGCTCACATTCCCTCACATTCCTTGCTTTCCACCACAACAATTTATCCGGCTCAATGCCGAGCTCTTTAGGCCAGTTGAATGAGCTTCAAGAGCTTGAACTTGGAAATAACCAGTTCAATGGAGCTATACCGAATGAAATAGGGCGGCTTTCGAGAGTTAGAATGTTAGATTTTTCCAACAATGCCATCAATGGAAGCTTGCCTTCTAGCTTCTCCAATCTCTCCTCGCTAATCCAGCTGAATTTAGAAGGAAACAACATCGAACGCGAAATCCCGGAAAGTCTTGGAAGTTTGAAAAACCTTTCTGTTCTTAACTTGAGGAAGAACAAATTGCAGGGTCCAATTCCTGCAGCTCTTGGGAACATTTCGACGCTTACCCGCCTCGATTTGTCGCTGAATAATGTCAGTGGTGAAATTCCAGCTTCTCTTGCTGGTTTATCACATCTTGTTTTCCTCAATGTTTCTTACAACAACCTCTCTGGTGCTGTCCCCGCTCCTCTCTCCCAGAAGTTCAATTCGAGCTCCTTTGTCGGGAATGTTCAGCTGTGCGGGTACAGTGCTTCAACTCCATGCCCTTCCAAGGCACCGTCTCAGAGTGTCCCGGCTCCAGGCCCGGAGACTGCGAAGCGCCGCCACAGACTTGGTACCAAAGACAAAATTCTTATAGCAGCAGGAGTTCTTTTGTTAGTGTTGTTTGTACTCTGTTGCATTCTGCTCTGCTGTTTGATAAGAAAAAGAGCTGCATCGAAAGCCAAGGATGGCACAGGCGCAGCAAGGGCTGGAGCTGCGAGGACCGAAAAGGGTGTTCCGGCCATTGGGGGAGAAGCTGAATCCGGAGGAGATGCTGGAGGAAAACTGGTACATTTTGACGGGCCAACGGCTTTCACAGCGGATGATCTTTTGTGCGCAACGGCAGAGATTATGGGGAAAAGCACTTTTGGGACTGTGTACAAGGCGACATTGGAGGATGGGAGTGAAGTTGCAGTGAAGAGATTGAGAGAAAAGATTACGAAAAGCCAGAGGGAATTCGAGACAGAAGTGAATGTGCTTGGGAAAATCAGGCACCCGAATCTTCTGGCTCTGAGGGCTTATTACTTGGGACCTAAGGGAGAAAAGCTTCTGGTTTTCGATTACATGCCGAGAGGGAGTCTTGCAGCATTTCTCCATGCTCGAGGACCGGATACTTCGATTGATTGGCCAACAAGAATGGGAATAGCAAAGGGCACGGCAAGAGGTCTCTCCTACCTCCACGCCAATGAGAACATTATCCATGGCAACCTCACATCCAGCAACATCCTACTTGATGAGCAGACGAATGCCAAGATCTCAGATTACGGCCTCTCCCGCCTCATGACGGCCGCTGCCAATTCCAACGTGATTGCAACCTCCGGAGCACTAGGGTACCGAGCCCCGGAGCTCTCAAAGCTCAAGAAGGCCAACACGAAAACTGATGTTTACAGCCTTGGGGTGATCATATTGGAACTCCTAACAGGGAAGTCTCCTGGAGAGCCGATGAACGGTTTGGATTTGCCTCAGTGGGTTGCTTCCATTGTGAAGGAGGAGTGGACCAACGAAGTTTTCGACTTGGAACTGATGAGGGACGCGTCTATCATCGGTGACGAGCTGCTAAACACACTGAAACTAGCTCTGCATTGTGTTGATCCTTCGCCATCGGCAAGGCCAGAAGTTCAACAGGTTCTGCAGCAGCTAGAAGAGATCAGACCCGAAACAGCCGCCAGTTCCGGCGACGACGGAGCTGAAGCCCCTTCAGCAAGTGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

877

Amino Acids

94.64

Weight (kDa)

8.87

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 123 - 162 1.7e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 188 - 316 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 238 - 297 1.9e-06 Leucine rich repeat
LRR_14 PF23598 305 - 389 1.6e-08 Leucine-rich repeat region
LRR_8 PF13855 310 - 369 6.8e-07 Leucine rich repeat
LRR_8 PF13855 382 - 441 1.1e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 587 - 852 3.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 587 - 851 3e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 487
Acc16I TGCGCA 1 cut(s) 1753
Acc36I ACCTGC 1 cut(s) 487
Acc65I GGTACC 3 cut(s) 630, 1490, 2247
AccB1I GGYRCC 5 cut(s) 630, 1429, 1490, 1908, 2247
AccBSI CCGCTC 2 cut(s) 849, 1340
AccII CGCG 3 cut(s) 279, 1113, 2450
AccIII TCCGGA 2 cut(s) 1679, 2234
AclWI GGATC 1 cut(s) 2501
AcoI YGGCCR 4 cut(s) 327, 1656, 2042, 2202
AcsI RAATTY 5 cut(s) 4, 1087, 1260, 1503, 1871
AcuI CTGAAG 2 cut(s) 2598, 2625
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 7 cut(s) 632, 1400, 1492, 1545, 1707, 1796, 2249
AflII CTTAAG 1 cut(s) 424
AflIII ACRYGT 1 cut(s) 2448
AjnI CCWGG 6 cut(s) 329, 518, 564, 760, 1453, 2347
AjuI GAANNNNNNNTTGG 4 cut(s) 44, 76, 2408, 2440
AloI GAACNNNNNNTCC 2 cut(s) 1101, 1133
Alw21I GWGCWC 7 cut(s) 151, 405, 510, 911, 1370, 2242, 2265
Alw26I GTCTC 5 cut(s) 1440, 1457, 1871, 2087, 2349
AlwI GGATC 1 cut(s) 2501
AlwNI CAGNNNCTG 2 cut(s) 571, 2545
Ama87I CYCGRG 2 cut(s) 832, 2016
Aor13HI TCCGGA 2 cut(s) 1679, 2234
ApoI RAATTY 5 cut(s) 4, 1087, 1260, 1503, 1871
Asp700I GAANNNNTTC 1 cut(s) 1962
Asp718I GGTACC 3 cut(s) 630, 1490, 2247
AspLEI GCGC 6 cut(s) 281, 488, 571, 1476, 1616, 1754
AsuC2I CCSGG 7 cut(s) 326, 833, 834, 1121, 1448, 1460, 2258
AsuHPI GGTGA 4 cut(s) 511, 1268, 2326, 2474
AvaI CYCGRG 2 cut(s) 832, 2016
AvaII GGWCC 5 cut(s) 1184, 1638, 1946, 2021, 2412
AxyI CCTNAGG 1 cut(s) 1950
BaeGI GKGCMC 1 cut(s) 2072
BaeI ACNNNNGTAYC 2 cut(s) 2231, 2264
BalI TGGCCA 1 cut(s) 2044
BanI GGYRCC 5 cut(s) 630, 1429, 1490, 1908, 2247
BanII GRGCYC 5 cut(s) 405, 911, 1370, 2257, 2265
BauI CACGAG 1 cut(s) 398
Bbv12I GWGCWC 7 cut(s) 151, 405, 510, 911, 1370, 2242, 2265
BbvCI CCTCAGC 1 cut(s) 572
BccI CCATC 6 cut(s) 214, 462, 1040, 1598, 1808, 2524
BceAI ACGGC 7 cut(s) 364, 378, 1740, 1773, 2088, 2197, 2217
BciT130I CCWGG 6 cut(s) 331, 520, 566, 762, 1455, 2349
BciVI GTATCC 1 cut(s) 2020
BclI TGATCA 1 cut(s) 2316
BcnI CCSGG 7 cut(s) 326, 833, 834, 1121, 1448, 1460, 2258
BcoDI GTCTC 5 cut(s) 1440, 1457, 1871, 2087, 2349
BfaI CTAG 5 cut(s) 114, 1053, 2243, 2489, 2555
BfmI CTRYAG 2 cut(s) 1194, 2546
BfoI RGCGCY 2 cut(s) 572, 1477
BfrI CTTAAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 1 cut(s) 2020
BglII AGATCT 2 cut(s) 28, 2169
BlpI GCTNAGC 1 cut(s) 581
Bme18I GGWCC 5 cut(s) 1184, 1638, 1946, 2021, 2412
BmeT110I CYCGRG 2 cut(s) 832, 2016
BmsI GCATC 2 cut(s) 1598, 1678
BpmI CTGGAG 7 cut(s) 494, 541, 548, 1437, 1647, 1713, 2370
Bpu10I CCTNAGC 2 cut(s) 489, 572
Bpu1102I GCTNAGC 1 cut(s) 581
BpuEI CTTGAG 2 cut(s) 1183, 2258
BpuMI CCSGG 7 cut(s) 326, 833, 834, 1121, 1448, 1460, 2258
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 2087
BsaJI CCNNGG 9 cut(s) 162, 518, 760, 832, 1425, 1599, 2116, 2256, 2308
BsaWI WCCGGW 3 cut(s) 1679, 2023, 2234
BsaXI ACNNNNNCTCC 2 cut(s) 2171, 2201
Bse1I ACTGG 4 cut(s) 919, 958, 1707, 2583
Bse21I CCTNAGG 1 cut(s) 1950
BseAI TCCGGA 2 cut(s) 1679, 2234
BseBI CCWGG 6 cut(s) 331, 520, 566, 762, 1455, 2349
BseDI CCNNGG 9 cut(s) 162, 518, 760, 832, 1425, 1599, 2116, 2256, 2308
BseGI GGATG 6 cut(s) 473, 1609, 1744, 1819, 2129, 2139
BseNI ACTGG 4 cut(s) 919, 958, 1707, 2583
BseRI GAGGAG 8 cut(s) 281, 296, 299, 663, 1060, 1332, 1698, 2420
BseSI GKGCMC 1 cut(s) 2072
BseX3I CGGCCG 1 cut(s) 2202
Bsh1236I CGCG 3 cut(s) 279, 1113, 2450
Bsh1285I CGRYCG 1 cut(s) 2205
BshNI GGYRCC 5 cut(s) 630, 1429, 1490, 1908, 2247
BsiEI CGRYCG 1 cut(s) 2205
BsiHKAI GWGCWC 7 cut(s) 151, 405, 510, 911, 1370, 2242, 2265
BsiHKCI CYCGRG 2 cut(s) 832, 2016
BslFI GGGAC 6 cut(s) 359, 1319, 1430, 1801, 1959, 2459
BsmAI GTCTC 5 cut(s) 1440, 1457, 1871, 2087, 2349
BsmBI CGTCTC 1 cut(s) 1440
BsmFI GGGAC 6 cut(s) 359, 1319, 1430, 1801, 1959, 2459
BsmI GAATGC 2 cut(s) 1554, 2167
Bso31I GGTCTC 1 cut(s) 2087
BsoBI CYCGRG 2 cut(s) 832, 2016
Bsp1286I GDGCHC 9 cut(s) 151, 405, 510, 911, 1370, 2072, 2242, 2257, 2265
Bsp13I TCCGGA 2 cut(s) 1679, 2234
Bsp1407I TGTACA 1 cut(s) 1794
Bsp143I GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
Bsp1720I GCTNAGC 1 cut(s) 581
Bsp19I CCATGG 2 cut(s) 162, 2116
BspEI TCCGGA 2 cut(s) 1679, 2234
BspFNI CGCG 3 cut(s) 279, 1113, 2450
BspHI TCATGA 1 cut(s) 2196
BspMAI CTGCAG 2 cut(s) 1198, 2550
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 1 cut(s) 2501
BspT107I GGYRCC 5 cut(s) 630, 1429, 1490, 1908, 2247
BspTI CTTAAG 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 2087
BsrBI CCGCTC 2 cut(s) 849, 1340
BsrGI TGTACA 1 cut(s) 1794
BsrI ACTGG 4 cut(s) 919, 958, 1707, 2583
BssECI CCNNGG 9 cut(s) 162, 518, 760, 832, 1425, 1599, 2116, 2256, 2308
BssMI GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
BssNI GRCGYC 1 cut(s) 351
BssSI CACGAG 1 cut(s) 398
BssT1I CCWWGG 5 cut(s) 162, 1425, 1599, 2116, 2308
Bst2BI CACGAG 1 cut(s) 398
Bst2UI CCWGG 6 cut(s) 331, 520, 566, 762, 1455, 2349
Bst4CI ACNGT 5 cut(s) 132, 1403, 1434, 1792, 2366
Bst6I CTCTTC 2 cut(s) 1829, 2553
BstACI GRCGYC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 424
BstAUI TGTACA 1 cut(s) 1794
BstC8I GCNNGC 2 cut(s) 457, 1046
BstDSI CCRYGG 2 cut(s) 162, 2116
BstF5I GGATG 6 cut(s) 473, 1609, 1744, 1819, 2129, 2139
BstFNI CGCG 3 cut(s) 279, 1113, 2450
BstH2I RGCGCY 2 cut(s) 572, 1477
BstHHI GCGC 6 cut(s) 281, 488, 571, 1476, 1616, 1754
BstKTI GATC 7 cut(s) 31, 730, 1744, 2172, 2319, 2509, 2565
BstMAI GTCTC 5 cut(s) 1440, 1457, 1871, 2087, 2349
BstMBI GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
BstMCI CGRYCG 1 cut(s) 2205
BstNI CCWGG 6 cut(s) 331, 520, 566, 762, 1455, 2349
BstNSI RCATGY 1 cut(s) 1984
BstSFI CTRYAG 2 cut(s) 1194, 2546
BstSLI GKGCMC 1 cut(s) 2072
BstUI CGCG 3 cut(s) 279, 1113, 2450
BstX2I RGATCY 2 cut(s) 28, 2169
BstXI CCANNNNNNTGG 2 cut(s) 965, 1487
BstYI RGATCY 2 cut(s) 28, 2169
BstZI CGGCCG 1 cut(s) 2202
Bsu36I CCTNAGG 1 cut(s) 1950
BsuI GTATCC 1 cut(s) 2020
BtgI CCRYGG 2 cut(s) 162, 2116
BtsCI GGATG 6 cut(s) 473, 1609, 1744, 1819, 2129, 2139
BtsI GCAGTG 1 cut(s) 1836
BtsIMutI CAGTG 5 cut(s) 1258, 1408, 1836, 2387, 2480
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 457, 1046
CaiI CAGNNNCTG 2 cut(s) 571, 2545
CciI TCATGA 1 cut(s) 2196
CfoI GCGC 6 cut(s) 281, 488, 571, 1476, 1616, 1754
Cfr9I CCCGGG 1 cut(s) 832
CseI GACGC 4 cut(s) 340, 1225, 2439, 2456
Csp6I GTAC 7 cut(s) 631, 1399, 1491, 1544, 1706, 1795, 2248
CviAII CATG 7 cut(s) 163, 590, 1416, 1981, 2012, 2117, 2197
CviQI GTAC 7 cut(s) 631, 1399, 1491, 1544, 1706, 1795, 2248
DpnI GATC 7 cut(s) 30, 729, 1743, 2171, 2318, 2508, 2564
DpnII GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
EaeI YGGCCR 4 cut(s) 327, 1656, 2042, 2202
EagI CGGCCG 1 cut(s) 2202
Eam1104I CTCTTC 2 cut(s) 1829, 2553
EarI CTCTTC 2 cut(s) 1829, 2553
EciI GGCGGA 2 cut(s) 383, 480
Ecl136II GAGCTC 4 cut(s) 403, 909, 1368, 2263
EclXI CGGCCG 1 cut(s) 2202
Eco130I CCWWGG 5 cut(s) 162, 1425, 1599, 2116, 2308
Eco24I GRGCYC 5 cut(s) 405, 911, 1370, 2257, 2265
Eco31I GGTCTC 1 cut(s) 2087
Eco47I GGWCC 5 cut(s) 1184, 1638, 1946, 2021, 2412
Eco52I CGGCCG 1 cut(s) 2202
Eco53kI GAGCTC 4 cut(s) 403, 909, 1368, 2263
Eco57I CTGAAG 2 cut(s) 2598, 2625
Eco81I CCTNAGG 1 cut(s) 1950
Eco88I CYCGRG 2 cut(s) 832, 2016
EcoICRI GAGCTC 4 cut(s) 403, 909, 1368, 2263
EcoO109I RGGNCCY 1 cut(s) 1946
EcoRI GAATTC 2 cut(s) 4, 1871
EcoRII CCWGG 6 cut(s) 329, 518, 564, 760, 1453, 2347
EcoT14I CCWWGG 5 cut(s) 162, 1425, 1599, 2116, 2308
EcoT38I GRGCYC 5 cut(s) 405, 911, 1370, 2257, 2265
ErhI CCWWGG 5 cut(s) 162, 1425, 1599, 2116, 2308
Esp3I CGTCTC 1 cut(s) 1440
FaeI CATG 7 cut(s) 166, 593, 1419, 1984, 2015, 2120, 2200
FalI AAGNNNNNCTT 4 cut(s) 407, 439, 1033, 1065
FaqI GGGAC 6 cut(s) 359, 1319, 1430, 1801, 1959, 2459
FatI CATG 7 cut(s) 162, 589, 1415, 1980, 2011, 2116, 2196
FauI CCCGC 6 cut(s) 196, 854, 1232, 1345, 1388, 2198
FbaI TGATCA 1 cut(s) 2316
FokI GGATG 6 cut(s) 480, 1616, 1751, 1826, 2116, 2126
FriOI GRGCYC 5 cut(s) 405, 911, 1370, 2257, 2265
FspBI CTAG 5 cut(s) 114, 1053, 2243, 2489, 2555
FspI TGCGCA 1 cut(s) 1753
GlaI GCGC 6 cut(s) 280, 487, 570, 1475, 1615, 1753
GsuI CTGGAG 7 cut(s) 494, 541, 548, 1437, 1647, 1713, 2370
HaeII RGCGCY 2 cut(s) 572, 1477
HgaI GACGC 4 cut(s) 340, 1225, 2439, 2456
HhaI GCGC 6 cut(s) 281, 488, 571, 1476, 1616, 1754
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 7 cut(s) 166, 593, 1419, 1984, 2015, 2120, 2200
Hin6I GCGC 6 cut(s) 279, 486, 569, 1474, 1614, 1752
HinP1I GCGC 6 cut(s) 279, 486, 569, 1474, 1614, 1752
HindIII AAGCTT 3 cut(s) 671, 1042, 1961
HinfI GANTC 6 cut(s) 117, 477, 540, 1676, 1915, 1993
HphI GGTGA 4 cut(s) 511, 1268, 2326, 2474
Hpy166II GTNNAC 3 cut(s) 1795, 2302, 2412
Hpy188III TCNNGA 9 cut(s) 114, 935, 999, 1378, 1680, 1876, 2197, 2235, 2275
Hpy8I GTNNAC 3 cut(s) 1795, 2302, 2412
Hpy99I CGWCG 4 cut(s) 410, 1219, 2597, 2600
HpyCH4III ACNGT 5 cut(s) 132, 1403, 1434, 1792, 2366
HpyCH4IV ACGT 1 cut(s) 2220
HpyCH4V TGCA 9 cut(s) 1180, 1196, 1554, 1589, 1829, 2000, 2228, 2497, 2548
HpySE526I ACGT 1 cut(s) 2220
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 7 cut(s) 166, 593, 1419, 1984, 2015, 2120, 2200
HspAI GCGC 6 cut(s) 279, 486, 569, 1474, 1614, 1752
Kpn2I TCCGGA 2 cut(s) 1679, 2234
KpnI GGTACC 3 cut(s) 634, 1494, 2251
Ksp22I TGATCA 1 cut(s) 2316
Kzo9I GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
LweI GCATC 2 cut(s) 1598, 1678
MaeI CTAG 5 cut(s) 114, 1053, 2243, 2489, 2555
MaeII ACGT 1 cut(s) 2220
MaeIII GTNAC 3 cut(s) 358, 446, 2462
MalI GATC 7 cut(s) 30, 729, 1743, 2171, 2318, 2508, 2564
MbiI CCGCTC 2 cut(s) 849, 1340
MboI GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
MboII GAAGA 7 cut(s) 250, 251, 310, 1180, 1846, 1910, 2570
MflI RGATCY 2 cut(s) 28, 2169
MhlI GDGCHC 9 cut(s) 151, 405, 510, 911, 1370, 2072, 2242, 2257, 2265
MlsI TGGCCA 1 cut(s) 2044
MluI ACGCGT 1 cut(s) 2448
MluNI TGGCCA 1 cut(s) 2044
MlyI GAGTC 1 cut(s) 2002
MmeI TCCRAC 4 cut(s) 222, 292, 1047, 2241
Mox20I TGGCCA 1 cut(s) 2044
MroI TCCGGA 2 cut(s) 1679, 2234
MroXI GAANNNNTTC 1 cut(s) 1962
MscI TGGCCA 1 cut(s) 2044
MseI TTAA 4 cut(s) 425, 663, 774, 1158
MslI CAYNNNNRTG 1 cut(s) 2100
Msp20I TGGCCA 1 cut(s) 2044
MspA1I CMGCKG 5 cut(s) 444, 1084, 1390, 1736, 2207
MspCI CTTAAG 1 cut(s) 424
Mva1269I GAATGC 2 cut(s) 1554, 2167
MvaI CCWGG 6 cut(s) 331, 520, 566, 762, 1455, 2349
MvnI CGCG 3 cut(s) 279, 1113, 2450
NciI CCSGG 7 cut(s) 326, 833, 834, 1121, 1448, 1460, 2258
NcoI CCATGG 2 cut(s) 162, 2116
NdeII GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
NlaIII CATG 7 cut(s) 166, 593, 1419, 1984, 2015, 2120, 2200
NmeAIII GCCGAG 2 cut(s) 930, 2010
NmuCI GTSAC 2 cut(s) 358, 2462
NsbI TGCGCA 1 cut(s) 1753
NspI RCATGY 1 cut(s) 1984
PaeR7I CTCGAG 1 cut(s) 2016
PagI TCATGA 1 cut(s) 2196
PaqCI CACCTGC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 2424
PctI GAATGC 2 cut(s) 1554, 2167
PdmI GAANNNNTTC 1 cut(s) 1962
PfeI GAWTC 5 cut(s) 117, 477, 540, 1676, 1915
PflFI GACNNNGTC 1 cut(s) 350
PfoI TCCNGGA 2 cut(s) 1119, 2347
PleI GAGTC 1 cut(s) 2001
PpsI GAGTC 1 cut(s) 2001
PpuMI RGGWCCY 1 cut(s) 1946
Psp124BI GAGCTC 4 cut(s) 405, 911, 1370, 2265
Psp5II RGGWCCY 1 cut(s) 1946
Psp6I CCWGG 6 cut(s) 329, 518, 564, 760, 1453, 2347
PspGI CCWGG 6 cut(s) 329, 518, 564, 760, 1453, 2347
PspPPI RGGWCCY 1 cut(s) 1946
PspXI VCTCGAGB 1 cut(s) 2016
PstI CTGCAG 2 cut(s) 1198, 2550
PstNI CAGNNNCTG 2 cut(s) 571, 2545
PsuI RGATCY 2 cut(s) 28, 2169
PsyI GACNNNGTC 1 cut(s) 350
PvuII CAGCTG 2 cut(s) 1084, 1390
RsaI GTAC 7 cut(s) 632, 1400, 1492, 1545, 1707, 1796, 2249
RsaNI GTAC 7 cut(s) 631, 1399, 1491, 1544, 1706, 1795, 2248
RseI CAYNNNNRTG 1 cut(s) 2100
SacI GAGCTC 4 cut(s) 405, 911, 1370, 2265
SaqAI TTAA 4 cut(s) 425, 663, 774, 1158
Sau3AI GATC 7 cut(s) 28, 727, 1741, 2169, 2316, 2506, 2562
SchI GAGTC 1 cut(s) 2002
SduI GDGCHC 9 cut(s) 151, 405, 510, 911, 1370, 2072, 2242, 2257, 2265
SfaNI GCATC 2 cut(s) 1598, 1678
SfcI CTRYAG 2 cut(s) 1194, 2546
Sfr274I CTCGAG 1 cut(s) 2016
SinI GGWCC 5 cut(s) 1184, 1638, 1946, 2021, 2412
SlaI CTCGAG 1 cut(s) 2016
SmaI CCCGGG 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 2100
SmlI CTYRAG 4 cut(s) 424, 1162, 2016, 2273
SmoI CTYRAG 4 cut(s) 424, 1162, 2016, 2273
SspMI CTAG 5 cut(s) 114, 1053, 2243, 2489, 2555
SstI GAGCTC 4 cut(s) 405, 911, 1370, 2265
StyI CCWWGG 5 cut(s) 162, 1425, 1599, 2116, 2308
TaaI ACNGT 5 cut(s) 132, 1403, 1434, 1792, 2366
TaiI ACGT 1 cut(s) 2223
TaqII GACCGA 1 cut(s) 1655
TatI WGTACW 2 cut(s) 1543, 1794
TauI GCSGC 5 cut(s) 368, 994, 1479, 2207, 2582
TfiI GAWTC 5 cut(s) 117, 477, 540, 1676, 1915
Tru1I TTAA 4 cut(s) 425, 663, 774, 1158
Tru9I TTAA 4 cut(s) 425, 663, 774, 1158
TscAI CASTG 5 cut(s) 1258, 1408, 1836, 2387, 2487
TseFI GTSAC 2 cut(s) 358, 2462
Tsp45I GTSAC 2 cut(s) 358, 2462
TspDTI ATGAA 3 cut(s) 17, 996, 2375
TspGWI ACGGA 2 cut(s) 465, 2613
TspMI CCCGGG 1 cut(s) 832
TspRI CASTG 5 cut(s) 1258, 1408, 1836, 2387, 2487
Tth111I GACNNNGTC 1 cut(s) 350
Vha464I CTTAAG 1 cut(s) 424
VpaK11BI GGWCC 5 cut(s) 1184, 1638, 1946, 2021, 2412
XapI RAATTY 5 cut(s) 4, 1087, 1260, 1503, 1871
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 1 cut(s) 1984
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 2016
XmaI CCCGGG 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 1962
XspI CTAG 5 cut(s) 114, 1053, 2243, 2489, 2555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.