pycom07g25580

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007019
Physical Location & Seq
Forward (+)
25851137 .. 25852910
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGGCGGAGCCAGAACCAACTACCGCGTCGCTGATGGACAAGAGCTACAGCCACCACTCGTCGTCTTCGTCCGACTCCGACGACGACAAGAAGGCCAAGCACGCTGCCGCCGTCAAAACCAAGGTTGAAGACAAGCCGTCGGCGCACGATTCCGTTAAATCCAAGATTTTCAGGCTCTTCGGGAGAGAAAAGCCCGTTCACAAGGTCTTGGGCGGCGGCAAACCTGCTGATGTTCTCCTATGGAGAAACAAGAAAATATCTGCAAGTGTGCTCGGCGGTGCCACTGTTTTATGGGTTTTCTTCGAGTTGCTCGAATACCACTTAATCACTCTGGTGTGCCATTTATTGATACTTTCTCTGGCGGTCTTTTTCCTGTGGTCCTATGCATCCACCTTTATCAACAAGTCGCCACCACAAATCCCAAAAGTTCAAATTCCTGAGAAGACTGTCCTTGAACTTGCCTCTGCTCTGAGAATTGAAATCAACAAAGGATTTTATGTTTTGCGGGATATTGCATCTGGAAGAGATTTGAAGACATTCCTTGGTGTTATAGCCGTCTTGTGGATTTTGTCGGTGGTTGGGAAATGGTTCAACTTTCTGACCCTATTCTACATAATTTTTGTGTTGCTGCACACTCTGCCGGTGATTTATGAGAAATACGACGACCAGATTGATGCCTTTGGTGAGAAGGCATGGATCGAAATTAAGAAGCAGTATGCAGTGTTTGATGCCAAGGTTTTGAGTAAAATCCCCAAGGGTCCAGTGAAGGAGAAGAAGAAGGATTAG

Protein Analysis

262

Amino Acids

29.42

Weight (kDa)

9.36

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 76 - 230 6.9e-60 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 232
AccB1I GGYRCC 1 cut(s) 278
AccII CGCG 1 cut(s) 26
AciI CCGC 8 cut(s) 5, 24, 108, 213, 216, 276, 362, 505
AclWI GGATC 1 cut(s) 704
AcsI RAATTY 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 561
AgsI TTSAA 6 cut(s) 128, 431, 455, 479, 532, 592
AhdI GACNNNNNGTC 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 332
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
Alw21I GWGCWC 1 cut(s) 273
AlwI GGATC 1 cut(s) 704
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 2 cut(s) 104, 628
ApoI RAATTY 1 cut(s) 432
Asp700I GAANNNNTTC 2 cut(s) 536, 587
AspLEI GCGC 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 378, 758
AsuHPI GGTGA 2 cut(s) 655, 695
AvaII GGWCC 2 cut(s) 378, 758
BanI GGYRCC 1 cut(s) 278
BbsI GAAGAC 4 cut(s) 57, 135, 449, 539
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 91, 615
BccI CCATC 1 cut(s) 28
BceAI ACGGC 3 cut(s) 95, 121, 539
BfmI CTRYAG 1 cut(s) 46
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 5 cut(s) 105, 108, 214, 217, 629
BlsI GCNGC 5 cut(s) 106, 109, 215, 218, 630
Bme18I GGWCC 2 cut(s) 378, 758
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgT120I GGNCC 2 cut(s) 378, 758
BmiI GGNNCC 3 cut(s) 9, 280, 759
BmsI GCATC 4 cut(s) 395, 524, 664, 718
BpiI GAAGAC 4 cut(s) 57, 135, 449, 539
BsaJI CCNNGG 4 cut(s) 120, 541, 732, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 1 cut(s) 761
BseDI CCNNGG 4 cut(s) 120, 541, 732, 753
BseGI GGATG 1 cut(s) 386
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 2 cut(s) 429, 461
BseNI ACTGG 1 cut(s) 761
BseXI GCAGC 2 cut(s) 91, 615
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 641
BslI CCNNNNNNNGG 1 cut(s) 561
BsnI GGCC 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 696
BspACI CCGC 8 cut(s) 5, 24, 108, 213, 216, 276, 362, 505
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 2 cut(s) 430, 462
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 3 cut(s) 9, 280, 759
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 1 cut(s) 704
BspQI GCTCTTC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 278
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 4 cut(s) 120, 541, 732, 753
BssMI GATC 1 cut(s) 696
BssT1I CCWWGG 4 cut(s) 120, 541, 732, 753
Bst4CI ACNGT 2 cut(s) 286, 448
Bst6I CTCTTC 2 cut(s) 182, 517
BstAPI GCANNNNNTGC 1 cut(s) 637
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 438, 470
BstF5I GGATG 1 cut(s) 386
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 1 cut(s) 699
BstMBI GATC 1 cut(s) 696
BstMWI GCNNNNNNNGC 3 cut(s) 101, 142, 637
BstSFI CTRYAG 1 cut(s) 46
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 91, 615
BstV2I GAAGAC 4 cut(s) 57, 135, 449, 539
BsuRI GGCC 1 cut(s) 95
BtsCI GGATG 1 cut(s) 386
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 3 cut(s) 282, 726, 768
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 378, 758
CseI GACGC 1 cut(s) 15
CviAII CATG 1 cut(s) 693
CviJI RGCY 8 cut(s) 10, 45, 51, 95, 136, 175, 193, 554
CviKI_1 RGCY 8 cut(s) 10, 45, 51, 95, 136, 175, 193, 554
DdeI CTNAG 2 cut(s) 438, 470
DpnI GATC 1 cut(s) 698
DpnII GATC 1 cut(s) 696
DriI GACNNNNNGTC 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 182, 517
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 2 cut(s) 182, 517
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 4 cut(s) 120, 541, 732, 753
Eco47I GGWCC 2 cut(s) 378, 758
EcoT14I CCWWGG 4 cut(s) 120, 541, 732, 753
EcoT22I ATGCAT 1 cut(s) 388
ErhI CCWWGG 4 cut(s) 120, 541, 732, 753
FaeI CATG 1 cut(s) 696
FaiI YATR 9 cut(s) 241, 292, 384, 498, 551, 614, 651, 694, 717
FatI CATG 1 cut(s) 692
FauI CCCGC 1 cut(s) 498
Fnu4HI GCNGC 5 cut(s) 105, 108, 214, 217, 629
FokI GGATG 1 cut(s) 373
Fsp4HI GCNGC 5 cut(s) 105, 108, 214, 217, 629
GlaI GCGC 1 cut(s) 144
GluI GCNGC 5 cut(s) 105, 108, 214, 217, 629
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 641
HgaI GACGC 1 cut(s) 15
HhaI GCGC 1 cut(s) 145
Hin1II CATG 1 cut(s) 696
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HinfI GANTC 2 cut(s) 74, 149
HpaII CCGG 1 cut(s) 641
HphI GGTGA 2 cut(s) 655, 695
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 4 cut(s) 73, 79, 471, 600
Hpy188III TCNNGA 3 cut(s) 181, 437, 519
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 6 cut(s) 31, 64, 83, 86, 142, 665
HpyAV CCTTC 4 cut(s) 85, 682, 760, 772
HpyCH4III ACNGT 2 cut(s) 286, 448
HpyCH4V TGCA 5 cut(s) 263, 386, 515, 631, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 142, 637
HpyF3I CTNAG 2 cut(s) 438, 470
Hsp92II CATG 1 cut(s) 696
HspAI GCGC 1 cut(s) 143
Kzo9I GATC 1 cut(s) 696
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 2 cut(s) 91, 615
LweI GCATC 4 cut(s) 395, 524, 664, 718
MalI GATC 1 cut(s) 698
MboI GATC 1 cut(s) 696
MboII GAAGA 8 cut(s) 57, 140, 169, 292, 454, 534, 544, 784
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 4 cut(s) 432, 474, 615, 702
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 2 cut(s) 96, 102
MnlI CCTC 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 388
MroXI GAANNNNTTC 2 cut(s) 536, 587
MseI TTAA 3 cut(s) 156, 323, 705
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 1 cut(s) 641
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 101, 142, 637
NdeII GATC 1 cut(s) 696
NlaIII CATG 1 cut(s) 696
NlaIV GGNNCC 3 cut(s) 9, 280, 759
NmeAIII GCCGAG 1 cut(s) 252
NsiI ATGCAT 1 cut(s) 388
OliI CACNNNNGTG 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 108, 309
PdmI GAANNNNTTC 2 cut(s) 536, 587
PfeI GAWTC 1 cut(s) 149
PkrI GCNGC 5 cut(s) 106, 109, 215, 218, 630
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PspN4I GGNNCC 3 cut(s) 9, 280, 759
PspPI GGNCC 2 cut(s) 378, 758
RseI CAYNNNNRTG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 3 cut(s) 156, 323, 705
SatI GCNGC 5 cut(s) 105, 108, 214, 217, 629
Sau3AI GATC 1 cut(s) 696
Sau96I GGNCC 2 cut(s) 378, 758
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 1 cut(s) 273
SetI ASST 6 cut(s) 47, 126, 207, 226, 395, 738
SfaNI GCATC 4 cut(s) 395, 524, 664, 718
SfcI CTRYAG 1 cut(s) 46
SinI GGWCC 2 cut(s) 378, 758
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 4 cut(s) 432, 474, 615, 702
SsiI CCGC 8 cut(s) 5, 24, 108, 213, 216, 276, 362, 505
StyI CCWWGG 4 cut(s) 120, 541, 732, 753
TaaI ACNGT 2 cut(s) 286, 448
TaqI TCGA 3 cut(s) 303, 312, 699
TasI AATT 4 cut(s) 432, 474, 615, 702
TauI GCSGC 3 cut(s) 110, 216, 219
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 3 cut(s) 156, 323, 705
Tru9I TTAA 3 cut(s) 156, 323, 705
TscAI CASTG 3 cut(s) 289, 726, 768
TseI GCWGC 2 cut(s) 104, 628
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 3 cut(s) 289, 726, 768
VpaK11BI GGWCC 2 cut(s) 378, 758
XapI RAATTY 1 cut(s) 432
XmnI GAANNNNTTC 2 cut(s) 536, 587
Zsp2I ATGCAT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.