pycom08g00620

At1g76070-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008592
Physical Location & Seq
Reverse (-)
513373 .. 514429
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGAAACGCAAGCTGTATTATTCAATTCAGTCGAGCAAATATGGTTTGCTAGTGCTGCTGGATAAGCGATTAAACACAATTTCATTATCCTCCTCAACGTCTAAAGCGTGCAACAAGATTAGCGTTATTCTTTTACATGCAACAATGGATCATCAGAAACAAGGTAAAACAAAAAACAAGATCCTGAAGTTTCTACCGAAAGCTACTTCCGGTGTTTCTTTTCACAGCCGTCCTTACAGCCCTACCAGAGAAGCCGCCGCCAAGCTTAAAGCTCATGGGCGGCGAGGGTTTTCCGGCCCCTTAATGATTCCAGATCAAGTCCGAAGAAAACAGAGAAACGGAAGCTTTGACGGTCAGACACAAGAGGAGGAGCCGACTTCGCCGAAAGTGTCGTGCATGGGACAGATCAAGCACAAGAAGCACATTAACAAGATAAATCGTGTTAATTTGATCCCCAAAGATCGCCAAAAATCAAAGCCTCCGGGGCCGTGGTCGCCGCGTGAGATCAAGAAGCACGGTTTTACGTTCCGGAGGATGTTTAGCAACATTAACCCCGGAAGAAAATCTGAATCTGATGCCAGTGATCTTGATCGTAGTCGGACGGCTCCATCTCTTCCGGATATGGCGCCTTCGTTGGGTCAGATGAAGAGATTTGCAAGCGGACGTGGCCATGTTTTCGAAAACTTCGAATGGGAATCTCCTCATAATATTATGAATAATCGTCGCGATTATTCGGATGAAGAGAGAGAAGACGGCGATGAGAGAGAAGAAGATGTTAGAATCGCATTCTCAGCGCCTCTAGGGATTGGAGGAAGAAATCAATTTCATCAAGTTCCTTTGCAGCCAAAGAAAGAAATCAATCTATGGAAGAGAAGAACAATGGCACCCCCTATTCCTCTAGAATTGAAACCCATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.96

Weight (kDa)

10.28

Isoelectric Point (pI)

62.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 625, 883
AccII CGCG 2 cut(s) 499, 726
AccIII TCCGGA 2 cut(s) 528, 616
AciI CCGC 5 cut(s) 255, 258, 280, 497, 660
AclWI GGATC 3 cut(s) 156, 175, 445
AcoI YGGCCR 1 cut(s) 667
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 626
AfiI CCNNNNNNNGG 1 cut(s) 635
AgsI TTSAA 2 cut(s) 24, 907
AjiI CACGTC 1 cut(s) 665
AluBI AGCT 5 cut(s) 13, 203, 265, 272, 345
AluI AGCT 5 cut(s) 13, 203, 265, 272, 345
AlwI GGATC 3 cut(s) 156, 175, 445
Aor13HI TCCGGA 2 cut(s) 528, 616
AoxI GGCC 3 cut(s) 295, 485, 667
ApeKI GCWGC 2 cut(s) 55, 841
AspLEI GCGC 2 cut(s) 628, 796
AspS9I GGNCC 2 cut(s) 296, 485
AsuC2I CCSGG 2 cut(s) 483, 555
AsuII TTCGAA 2 cut(s) 678, 687
BalI TGGCCA 1 cut(s) 669
BanI GGYRCC 2 cut(s) 625, 883
BbsI GAAGAC 1 cut(s) 756
BbvI GCAGC 2 cut(s) 42, 853
BccI CCATC 1 cut(s) 616
BceAI ACGGC 4 cut(s) 213, 472, 618, 769
BcnI CCSGG 2 cut(s) 483, 555
BfaI CTAG 3 cut(s) 50, 800, 899
BfoI RGCGCY 2 cut(s) 629, 797
BglI GCCNNNNNGGC 1 cut(s) 484
BisI GCNGC 6 cut(s) 56, 255, 258, 281, 497, 842
BlsI GCNGC 6 cut(s) 57, 256, 259, 282, 498, 843
Bme1390I CCNGG 2 cut(s) 483, 555
BmgBI CACGTC 1 cut(s) 665
BmgT120I GGNCC 2 cut(s) 296, 485
BmiI GGNNCC 6 cut(s) 298, 372, 486, 606, 627, 885
BmrFI CCNGG 2 cut(s) 483, 555
BmsI GCATC 1 cut(s) 565
BpiI GAAGAC 1 cut(s) 756
Bpu14I TTCGAA 2 cut(s) 678, 687
BpuMI CCSGG 2 cut(s) 483, 555
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 626
BsaJI CCNNGG 4 cut(s) 482, 488, 553, 912
BsaWI WCCGGW 3 cut(s) 209, 528, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 579
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 2 cut(s) 528, 616
BseDI CCNNGG 4 cut(s) 482, 488, 553, 912
BseGI GGATG 2 cut(s) 540, 742
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 579
BseRI GAGGAG 4 cut(s) 82, 380, 383, 690
BseXI GCAGC 2 cut(s) 42, 853
Bsh1236I CGCG 2 cut(s) 499, 726
BshFI GGCC 3 cut(s) 297, 487, 669
BshNI GGYRCC 2 cut(s) 625, 883
BsiSI CCGG 6 cut(s) 210, 294, 482, 529, 555, 617
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 414
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 3 cut(s) 297, 487, 669
Bsp119I TTCGAA 2 cut(s) 678, 687
Bsp13I TCCGGA 2 cut(s) 528, 616
Bsp143I GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
Bsp19I CCATGG 1 cut(s) 912
Bsp68I TCGCGA 1 cut(s) 726
BspACI CCGC 5 cut(s) 255, 258, 280, 497, 660
BspANI GGCC 3 cut(s) 297, 487, 669
BspCNI CTCAG 1 cut(s) 803
BspEI TCCGGA 2 cut(s) 528, 616
BspFNI CGCG 2 cut(s) 499, 726
BspLI GGNNCC 6 cut(s) 298, 372, 486, 606, 627, 885
BspPI GGATC 3 cut(s) 156, 175, 445
BspT104I TTCGAA 2 cut(s) 678, 687
BspT107I GGYRCC 2 cut(s) 625, 883
BsrI ACTGG 1 cut(s) 579
BssECI CCNNGG 4 cut(s) 482, 488, 553, 912
BssMI GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
BssNI GRCGYC 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 912
Bst4CI ACNGT 2 cut(s) 353, 518
Bst6I CTCTTC 4 cut(s) 618, 641, 735, 863
BstACI GRCGYC 1 cut(s) 626
BstBI TTCGAA 2 cut(s) 678, 687
BstC8I GCNNGC 3 cut(s) 11, 109, 658
BstDEI CTNAG 1 cut(s) 790
BstDSI CCRYGG 2 cut(s) 488, 912
BstF5I GGATG 2 cut(s) 540, 742
BstFNI CGCG 2 cut(s) 499, 726
BstH2I RGCGCY 2 cut(s) 629, 797
BstHHI GCGC 2 cut(s) 628, 796
BstKTI GATC 9 cut(s) 151, 183, 316, 408, 453, 463, 507, 586, 592
BstMBI GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
BstMWI GCNNNNNNNGC 8 cut(s) 55, 64, 379, 418, 484, 493, 666, 791
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 481, 553
BstUI CGCG 2 cut(s) 499, 726
BstV1I GCAGC 2 cut(s) 42, 853
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 3 cut(s) 297, 487, 669
BtgI CCRYGG 2 cut(s) 488, 912
BtgZI GCGATG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 665
BtsCI GGATG 2 cut(s) 540, 742
BtsIMutI CAGTG 1 cut(s) 586
BtuMI TCGCGA 1 cut(s) 726
Cac8I GCNNGC 3 cut(s) 11, 109, 658
CfoI GCGC 2 cut(s) 628, 796
Cfr13I GGNCC 2 cut(s) 296, 485
CviAII CATG 5 cut(s) 137, 275, 397, 671, 913
DdeI CTNAG 1 cut(s) 790
DinI GGCGCC 1 cut(s) 627
DpnI GATC 9 cut(s) 150, 182, 315, 407, 452, 462, 506, 585, 591
DpnII GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 4 cut(s) 618, 641, 735, 863
EarI CTCTTC 4 cut(s) 618, 641, 735, 863
Eco130I CCWWGG 1 cut(s) 912
Eco57I CTGAAG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 912
EgeI GGCGCC 1 cut(s) 627
EheI GGCGCC 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 912
FaeI CATG 5 cut(s) 140, 278, 400, 674, 916
FaqI GGGAC 1 cut(s) 414
FatI CATG 5 cut(s) 136, 274, 396, 670, 912
Fnu4HI GCNGC 6 cut(s) 56, 255, 258, 281, 497, 842
FokI GGATG 2 cut(s) 547, 749
Fsp4HI GCNGC 6 cut(s) 56, 255, 258, 281, 497, 842
FspBI CTAG 3 cut(s) 50, 800, 899
GlaI GCGC 2 cut(s) 627, 795
GluI GCNGC 6 cut(s) 56, 255, 258, 281, 497, 842
HaeII RGCGCY 2 cut(s) 629, 797
HaeIII GGCC 3 cut(s) 297, 487, 669
HapII CCGG 6 cut(s) 210, 294, 482, 529, 555, 617
HhaI GCGC 2 cut(s) 628, 796
Hin1I GRCGYC 1 cut(s) 626
Hin1II CATG 5 cut(s) 140, 278, 400, 674, 916
Hin6I GCGC 2 cut(s) 626, 794
HinP1I GCGC 2 cut(s) 626, 794
HindIII AAGCTT 2 cut(s) 263, 343
HinfI GANTC 4 cut(s) 307, 569, 695, 780
HpaII CCGG 6 cut(s) 210, 294, 482, 529, 555, 617
Hpy188I TCNGA 8 cut(s) 156, 323, 357, 568, 574, 600, 642, 736
Hpy188III TCNNGA 8 cut(s) 184, 311, 508, 529, 587, 617, 725, 899
Hpy99I CGWCG 1 cut(s) 726
HpyAV CCTTC 1 cut(s) 639
HpyCH4III ACNGT 2 cut(s) 353, 518
HpyCH4IV ACGT 3 cut(s) 98, 524, 664
HpyCH4V TGCA 5 cut(s) 111, 140, 396, 656, 841
HpyF10VI GCNNNNNNNGC 8 cut(s) 55, 64, 379, 418, 484, 493, 666, 791
HpyF3I CTNAG 1 cut(s) 790
HpySE526I ACGT 3 cut(s) 98, 524, 664
Hsp92I GRCGYC 1 cut(s) 626
Hsp92II CATG 5 cut(s) 140, 278, 400, 674, 916
HspAI GCGC 2 cut(s) 626, 794
KasI GGCGCC 1 cut(s) 625
Kpn2I TCCGGA 2 cut(s) 528, 616
Kzo9I GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
LmnI GCTCC 2 cut(s) 370, 610
Lsp1109I GCAGC 2 cut(s) 42, 853
LweI GCATC 1 cut(s) 565
MaeI CTAG 3 cut(s) 50, 800, 899
MaeII ACGT 3 cut(s) 98, 524, 664
MalI GATC 9 cut(s) 150, 182, 315, 407, 452, 462, 506, 585, 591
MboI GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
MflI RGATCY 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 5 cut(s) 24, 78, 445, 821, 902
MluNI TGGCCA 1 cut(s) 669
Mly113I GGCGCC 1 cut(s) 626
MmeI TCCRAC 1 cut(s) 578
Mox20I TGGCCA 1 cut(s) 669
MroI TCCGGA 2 cut(s) 528, 616
MscI TGGCCA 1 cut(s) 669
MseI TTAA 6 cut(s) 71, 267, 302, 426, 444, 549
Msp20I TGGCCA 1 cut(s) 669
MspI CCGG 6 cut(s) 210, 294, 482, 529, 555, 617
MspR9I CCNGG 2 cut(s) 483, 555
Mva1269I GAATGC 1 cut(s) 785
MvnI CGCG 2 cut(s) 499, 726
MwoI GCNNNNNNNGC 8 cut(s) 55, 64, 379, 418, 484, 493, 666, 791
NarI GGCGCC 1 cut(s) 626
NciI CCSGG 2 cut(s) 483, 555
NcoI CCATGG 1 cut(s) 912
NdeII GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
NlaIII CATG 5 cut(s) 140, 278, 400, 674, 916
NlaIV GGNNCC 6 cut(s) 298, 372, 486, 606, 627, 885
NruI TCGCGA 1 cut(s) 726
NspI RCATGY 1 cut(s) 140
NspV TTCGAA 2 cut(s) 678, 687
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 684
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 4 cut(s) 307, 569, 695, 780
PkrI GCNGC 6 cut(s) 57, 256, 259, 282, 498, 843
PluTI GGCGCC 1 cut(s) 629
PspN4I GGNNCC 6 cut(s) 298, 372, 486, 606, 627, 885
PspPI GGNCC 2 cut(s) 296, 485
PsuI RGATCY 1 cut(s) 180
RruI TCGCGA 1 cut(s) 726
SaqAI TTAA 6 cut(s) 71, 267, 302, 426, 444, 549
SatI GCNGC 6 cut(s) 56, 255, 258, 281, 497, 842
Sau3AI GATC 9 cut(s) 148, 180, 313, 405, 450, 460, 504, 583, 589
Sau96I GGNCC 2 cut(s) 296, 485
ScrFI CCNGG 2 cut(s) 483, 555
SetI ASST 9 cut(s) 15, 101, 166, 205, 267, 274, 347, 527, 667
SfaNI GCATC 1 cut(s) 565
SfoI GGCGCC 1 cut(s) 627
SfuI TTCGAA 2 cut(s) 678, 687
Sse9I AATT 5 cut(s) 24, 78, 445, 821, 902
SsiI CCGC 5 cut(s) 255, 258, 280, 497, 660
SspDI GGCGCC 1 cut(s) 625
SspI AATATT 1 cut(s) 709
SspMI CTAG 3 cut(s) 50, 800, 899
StyD4I CCNGG 2 cut(s) 481, 553
StyI CCWWGG 1 cut(s) 912
TaaI ACNGT 2 cut(s) 353, 518
TaiI ACGT 3 cut(s) 101, 527, 667
TaqI TCGA 3 cut(s) 32, 678, 687
TasI AATT 5 cut(s) 24, 78, 445, 821, 902
TauI GCSGC 4 cut(s) 257, 260, 283, 499
TfiI GAWTC 4 cut(s) 307, 569, 695, 780
Tru1I TTAA 6 cut(s) 71, 267, 302, 426, 444, 549
Tru9I TTAA 6 cut(s) 71, 267, 302, 426, 444, 549
TscAI CASTG 1 cut(s) 586
TseI GCWGC 2 cut(s) 55, 841
TspDTI ATGAA 6 cut(s) 17, 72, 659, 728, 753, 815
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 1 cut(s) 586
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 1 cut(s) 140
XspI CTAG 3 cut(s) 50, 800, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.