pycom08g06230

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003573
Physical Location & Seq
Reverse (-)
4823606 .. 4824897
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06230.3

Sequence Viewer

Length: 912 bp
ATGGGTTTTGATCATGAAGCTTGTAACACTGGCCTTGGACTCAGCCTAGGTTGTCAAGGTAATCCAGATCATCATACAAATTTGCAGGCTTTCGATCTCGATCACCAAAAGAAGAAGAAAATACAGTTGAAATACGATCACCTATTGCCGGCTCTAACCCTAGGTCCATCGTCCGGGGCTAAGATCGAGGCCGCCAAATCGGATGATCATTTGCACCAAGTAGTATATGTACAAGCACAAGAACAGGCCTCTTCTCCCTGCAGCGGCGCCGTTTCTTCGTTCTCCAACTCATCTAGTTTCAAGAGGGACAGAGGTTTGGGTGTTGAAGAGATAGAGGGAGAGGTAGAAGAAGAGAGAGTAGTGAATATCACTACTTCATCAAGAGTAAGCGACGAATTATATGATCAAGATCAAGATCACGAAGGCAGTCCTAGAAAGAAACTCAGACTTACAAAAGAACAATCCGCCACTTTAGAGGACACCTTCAGAGAGCACACTACTCTCAATCCAAAACAAAAACAAGAACTGGCAAGAAAGCTAAACTTACGCCCACGACAAGTTGAAGTCTGGTTCCAAAACAGGAGGGCTAGGACCAAGCTGAAGCAAACGGAAGCAGAGTGCGAGTTGTTGAAGCAATGTTGTGAAACATTGAAGGAAGAGAACAGAAGGCTACACAAGGAGCTGCAAGAGCTCAAATTAATGAAACAAACAGCAACAGCAGCAGCACCCTTTTACATGCAGTTTCCAACTGCCACTCTCACCATGTGTCCCTCCTGTGAGAAAATCTGCAACGTCGGCGATCATCATAATCATAATCACAACGAGTCATCGACCAGTCCATTTTTAGTCGGGTCGAATAAGACTCACTTGATCTTCAACCCCTACAGTACTCATCCATCTACAGCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HALZ Homeobox

Protein Analysis

304

Amino Acids

34.19

Weight (kDa)

6.6

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AciI CCGC 3 cut(s) 192, 264, 465
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 2 cut(s) 469, 620
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 765
AfaI GTAC 2 cut(s) 231, 889
AfiI CCNNNNNNNGG 3 cut(s) 148, 173, 263
AgsI TTSAA 7 cut(s) 130, 301, 326, 563, 631, 652, 877
AluBI AGCT 6 cut(s) 20, 538, 598, 682, 691, 905
AluI AGCT 6 cut(s) 20, 538, 598, 682, 691, 905
Alw21I GWGCWC 2 cut(s) 495, 693
AoxI GGCC 3 cut(s) 31, 189, 246
ApeKI GCWGC 4 cut(s) 261, 682, 719, 722
ApoI RAATTY 1 cut(s) 79
AseI ATTAAT 1 cut(s) 698
AspA2I CCTAGG 2 cut(s) 46, 160
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 164, 591
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 95, 131, 751
AvaII GGWCC 2 cut(s) 164, 591
AvrII CCTAGG 2 cut(s) 46, 160
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 495, 693
BbvI GCAGC 4 cut(s) 273, 669, 731, 734
BccI CCATC 2 cut(s) 175, 904
BceAI ACGGC 1 cut(s) 254
BclI TGATCA 3 cut(s) 10, 205, 403
BcnI CCSGG 1 cut(s) 175
BfaI CTAG 6 cut(s) 47, 161, 294, 432, 588, 910
BfmI CTRYAG 3 cut(s) 259, 883, 900
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 6 cut(s) 192, 262, 265, 683, 720, 723
BlnI CCTAGG 2 cut(s) 46, 160
BlsI GCNGC 6 cut(s) 193, 263, 266, 684, 721, 724
BmcAI AGTACT 1 cut(s) 889
Bme1390I CCNGG 1 cut(s) 175
Bme18I GGWCC 2 cut(s) 164, 591
BmgT120I GGNCC 2 cut(s) 164, 591
BmiI GGNNCC 2 cut(s) 268, 572
BmrFI CCNGG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 3 cut(s) 99, 408, 414
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 34, 46, 160, 174
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 173, 263
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 3 cut(s) 34, 531, 834
Bse3DI GCAATG 1 cut(s) 641
Bse8I GATNNNNATC 3 cut(s) 99, 408, 414
BseDI CCNNGG 4 cut(s) 34, 46, 160, 174
BseGI GGATG 2 cut(s) 208, 892
BseJI GATNNNNATC 3 cut(s) 99, 408, 414
BseLI CCNNNNNNNGG 3 cut(s) 148, 173, 263
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 55, 457
BseNI ACTGG 3 cut(s) 34, 531, 834
BseXI GCAGC 4 cut(s) 273, 669, 731, 734
BshFI GGCC 3 cut(s) 33, 191, 248
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 2 cut(s) 495, 693
BsiSI CCGG 2 cut(s) 149, 174
BslFI GGGAC 2 cut(s) 320, 753
BslI CCNNNNNNNGG 3 cut(s) 148, 173, 263
BsmFI GGGAC 2 cut(s) 320, 753
BsnI GGCC 3 cut(s) 33, 191, 248
Bsp1286I GDGCHC 2 cut(s) 495, 693
Bsp1407I TGTACA 1 cut(s) 229
BspACI CCGC 3 cut(s) 192, 264, 465
BspANI GGCC 3 cut(s) 33, 191, 248
BspCNI CTCAG 2 cut(s) 54, 456
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 268, 572
BspMAI CTGCAG 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 641
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 229
BsrI ACTGG 3 cut(s) 34, 531, 834
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 34, 46, 160, 174
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 3 cut(s) 34, 46, 160
Bst4CI ACNGT 2 cut(s) 126, 887
Bst6I CTCTTC 4 cut(s) 256, 321, 345, 651
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 229
BstC8I GCNNGC 3 cut(s) 87, 150, 907
BstDEI CTNAG 3 cut(s) 41, 180, 443
BstF5I GGATG 2 cut(s) 208, 892
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstMWI GCNNNNNNNGC 3 cut(s) 688, 719, 795
BstNSI RCATGY 1 cut(s) 739
BstSCI CCNGG 1 cut(s) 173
BstSFI CTRYAG 3 cut(s) 259, 883, 900
BstV1I GCAGC 4 cut(s) 273, 669, 731, 734
BsuRI GGCC 3 cut(s) 33, 191, 248
BtsCI GGATG 2 cut(s) 208, 892
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 87, 150, 907
CciI TCATGA 1 cut(s) 13
CfoI GCGC 1 cut(s) 269
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 164, 591
Csp6I GTAC 2 cut(s) 230, 888
CviAII CATG 3 cut(s) 14, 736, 763
CviQI GTAC 2 cut(s) 230, 888
DdeI CTNAG 3 cut(s) 41, 180, 443
DinI GGCGCC 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 765
Eam1104I CTCTTC 4 cut(s) 256, 321, 345, 651
EarI CTCTTC 4 cut(s) 256, 321, 345, 651
EciI GGCGGA 1 cut(s) 454
Ecl136II GAGCTC 1 cut(s) 691
Eco130I CCWWGG 3 cut(s) 34, 46, 160
Eco147I AGGCCT 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 693
Eco47I GGWCC 2 cut(s) 164, 591
Eco53kI GAGCTC 1 cut(s) 691
Eco57I CTGAAG 2 cut(s) 469, 620
EcoICRI GAGCTC 1 cut(s) 691
EcoT14I CCWWGG 3 cut(s) 34, 46, 160
EcoT38I GRGCYC 1 cut(s) 693
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 3 cut(s) 34, 46, 160
FaeI CATG 3 cut(s) 17, 739, 766
FalI AAGNNNNNCTT 4 cut(s) 527, 559, 851, 883
FaqI GGGAC 2 cut(s) 320, 753
FatI CATG 3 cut(s) 13, 735, 762
FbaI TGATCA 3 cut(s) 10, 205, 403
Fnu4HI GCNGC 6 cut(s) 192, 262, 265, 683, 720, 723
FokI GGATG 2 cut(s) 215, 879
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 6 cut(s) 192, 262, 265, 683, 720, 723
FspBI CTAG 6 cut(s) 47, 161, 294, 432, 588, 910
GlaI GCGC 1 cut(s) 268
GluI GCNGC 6 cut(s) 192, 262, 265, 683, 720, 723
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 33, 191, 248
HapII CCGG 2 cut(s) 149, 174
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 17, 739, 766
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 3 cut(s) 39, 824, 862
HpaII CCGG 2 cut(s) 149, 174
HphI GGTGA 3 cut(s) 95, 131, 751
Hpy188I TCNGA 3 cut(s) 202, 446, 488
Hpy188III TCNNGA 8 cut(s) 14, 65, 98, 301, 381, 407, 413, 419
Hpy99I CGWCG 2 cut(s) 395, 797
HpyAV CCTTC 4 cut(s) 416, 493, 646, 660
HpyCH4III ACNGT 2 cut(s) 126, 887
HpyCH4IV ACGT 1 cut(s) 792
HpyCH4V TGCA 6 cut(s) 85, 214, 261, 685, 739, 789
HpyF10VI GCNNNNNNNGC 3 cut(s) 688, 719, 795
HpyF3I CTNAG 3 cut(s) 41, 180, 443
HpySE526I ACGT 1 cut(s) 792
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 17, 739, 766
HspAI GCGC 1 cut(s) 267
KasI GGCGCC 1 cut(s) 266
KroI GCCGGC 1 cut(s) 148
KroNI GCCGGC 1 cut(s) 150
Ksp22I TGATCA 3 cut(s) 10, 205, 403
LmnI GCTCC 1 cut(s) 679
Lsp1109I GCAGC 4 cut(s) 273, 669, 731, 734
MaeI CTAG 6 cut(s) 47, 161, 294, 432, 588, 910
MaeII ACGT 1 cut(s) 792
MaeIII GTNAC 1 cut(s) 23
MboII GAAGA 9 cut(s) 124, 127, 243, 267, 338, 359, 362, 668, 865
MhlI GDGCHC 2 cut(s) 495, 693
MluCI AATT 3 cut(s) 79, 395, 695
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 3 cut(s) 33, 833, 856
MmeI TCCRAC 2 cut(s) 309, 770
MnlI CCTC 9 cut(s) 181, 259, 297, 305, 328, 334, 469, 576, 781
MroNI GCCGGC 1 cut(s) 148
MseI TTAA 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 2 cut(s) 149, 174
MspR9I CCNGG 1 cut(s) 175
MwoI GCNNNNNNNGC 3 cut(s) 688, 719, 795
NaeI GCCGGC 1 cut(s) 150
NarI GGCGCC 1 cut(s) 267
NciI CCSGG 1 cut(s) 175
NgoMIV GCCGGC 1 cut(s) 148
NlaIII CATG 3 cut(s) 17, 739, 766
NlaIV GGNNCC 2 cut(s) 268, 572
NspI RCATGY 1 cut(s) 739
PagI TCATGA 1 cut(s) 13
PceI AGGCCT 1 cut(s) 248
PdiI GCCGGC 1 cut(s) 150
PkrI GCNGC 6 cut(s) 193, 263, 266, 684, 721, 724
PleI GAGTC 3 cut(s) 33, 832, 856
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 3 cut(s) 33, 832, 856
PshBI ATTAAT 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 693
PspN4I GGNNCC 2 cut(s) 268, 572
PspPI GGNCC 2 cut(s) 164, 591
PstI CTGCAG 1 cut(s) 263
RsaI GTAC 2 cut(s) 231, 889
RsaNI GTAC 2 cut(s) 230, 888
SacI GAGCTC 1 cut(s) 693
SaqAI TTAA 1 cut(s) 698
SatI GCNGC 6 cut(s) 192, 262, 265, 683, 720, 723
Sau96I GGNCC 2 cut(s) 164, 591
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 3 cut(s) 33, 833, 856
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 2 cut(s) 495, 693
SfcI CTRYAG 3 cut(s) 259, 883, 900
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 2 cut(s) 164, 591
Sse9I AATT 3 cut(s) 79, 395, 695
SseBI AGGCCT 1 cut(s) 248
SsiI CCGC 3 cut(s) 192, 264, 465
SspDI GGCGCC 1 cut(s) 266
SspMI CTAG 6 cut(s) 47, 161, 294, 432, 588, 910
SstI GAGCTC 1 cut(s) 693
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 173
StyI CCWWGG 3 cut(s) 34, 46, 160
TaaI ACNGT 2 cut(s) 126, 887
TaiI ACGT 1 cut(s) 795
TaqI TCGA 5 cut(s) 93, 99, 186, 830, 854
TasI AATT 3 cut(s) 79, 395, 695
TatI WGTACW 2 cut(s) 229, 887
TauI GCSGC 2 cut(s) 194, 267
Tru1I TTAA 1 cut(s) 698
Tru9I TTAA 1 cut(s) 698
TscAI CASTG 1 cut(s) 34
TseI GCWGC 4 cut(s) 261, 682, 719, 722
TspDTI ATGAA 3 cut(s) 30, 366, 716
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 164, 591
VspI ATTAAT 1 cut(s) 698
XapI RAATTY 1 cut(s) 79
XceI RCATGY 1 cut(s) 739
XmaJI CCTAGG 2 cut(s) 46, 160
XspI CTAG 6 cut(s) 47, 161, 294, 432, 588, 910
ZrmI AGTACT 1 cut(s) 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.