pycom08g16040

Phospho-2-dehydro-3-deoxyheptonate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012417
Physical Location & Seq
Forward (+)
15940086 .. 15942482
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16040.1

Sequence Viewer

Length: 1284 bp
ATGTTGAAAATACATCAAAAATTTGAAGGAAAAAATTTTTTTTTTTTTTTTATAAACGATATTATTTATATTAAGAGGAAGGATAATTGGTTTAGCCATTTAATTATGTTGAAACGCTTAATTCCAAAAATACCCTTCCAACAACCCGCAAAACCCCGGAACAACCCACACTCGTCCCCTCAACTGCCCAACACCGCATCTCTCTGTAAAGACACCCTGTTTTCCTATAAAAGCCCTCCCAATTTCCTTTTCCTTCCTCAGGTACCCTCTGAGATTTCTCTCTCCAATCTCATTGTACCTTCACACCAAATAGAAAAAGGAAAAAAAAAAAAGAAAAAATCTTCAGAAAATCTAACAAAAATGGCGCTCACAAGCAGCAGCGCTCTGATTCCCACCAAATCCGGCTTCGTTCAAGGTCAGTCTCTCTTACCCTTTTCCAGAACTCACCAACCCTCTCTGCCCATCAAGTCCGTCTGGCCAATCGTCGCCGTCCACGCCGCCGAGCCAGCCAAGAACCCAGTTGTCTCCGATAAGCCGCCGAAGCAGCAGGCTGCTCAGACGGCGTCTTCTTCTTCTTTGGCGACGAAGCACAATGCAGTTCCGGCGAAATGGAGCTTGGAGACATGGAAATCTAAGAAGGCGCTGCAATTGCCGGAGTACCCGAATCAGGAAGATCTCCAGTCGGTGCTTCGGACCCTCGATTCGTTCCCGCCTATTGTGTTCGCTGGTGAGGCGAGGAACCTGGAGGAGCTTATCGGAGAGGCCGCCATGGGAAACGCGTTTCTTCTTCAAGGTGGGGACTGCGCTGAGAGCTTCAAGGAGTTCAATGCCAACAACATTCGTGACACCTTCAGAATTCTCCTCCAAATGGGCGTCGTTTTGATGTTCGGAGGCCAAATGCCCGTCGTCAAGGTGGGAAGAATGGCGGGTCAGTTTGCGAAACCCAGATCGGATTCTTTTGAGGAGAAGAATGGTGTGAAGCTGCCTAGTTACAGAGGGGATAATGTGAACGGAGACGCCTTCGATTTAGAGTCGAGGACTCCAGACCCCCAGAGACTGATTAGAGCCTATTGTCAATCTGCTGCTACTCTCAACCTTCTCAGGGCTTTCGCTACTGGAGGTTATGCAGCTATGCAGAGGGTCACACAGTGGAACTTGGATTTCACGGAGCACAGCGAGCAAGGTGATAGATATCGGGAGCTAGCTTCCAGGGTTGATGAGGCCCTAGGATTCATGACCGCTGCAGGGCTCACAATCGATCGACCCCATCATGACAACAACTGA

Protein Analysis

428

Amino Acids

47.71

Weight (kDa)

9.56

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAHP_synth_2 PF01474 204 - 419 1.3e-87 Class-II DAHP synthetase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 53
Acc65I GGTACC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 262
AccII CGCG 1 cut(s) 779
AciI CCGC 8 cut(s) 147, 195, 498, 536, 710, 765, 926, 1239
AcoI YGGCCR 1 cut(s) 476
AcsI RAATTY 3 cut(s) 20, 34, 855
AcuI CTGAAG 2 cut(s) 327, 835
AcyI GRCGYC 3 cut(s) 563, 873, 1017
AdeI CACNNNGTG 1 cut(s) 1149
AfaI GTAC 3 cut(s) 264, 297, 659
AfeI AGCGCT 1 cut(s) 382
AfiI CCNNNNNNNGG 5 cut(s) 259, 667, 868, 1102, 1245
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 7 cut(s) 7, 26, 112, 413, 791, 817, 826
AjnI CCWGG 2 cut(s) 741, 1208
AjuI GAANNNNNNNTTGG 6 cut(s) 233, 265, 389, 421, 599, 631
AluBI AGCT 7 cut(s) 615, 751, 813, 982, 1130, 1201, 1205
AluI AGCT 7 cut(s) 615, 751, 813, 982, 1130, 1201, 1205
Alw21I GWGCWC 1 cut(s) 1173
Alw26I GTCTC 5 cut(s) 426, 529, 614, 1008, 1048
AlwNI CAGNNNCTG 1 cut(s) 1057
Aor51HI AGCGCT 1 cut(s) 382
AoxI GGCC 4 cut(s) 476, 762, 892, 1221
ApeKI GCWGC 9 cut(s) 375, 378, 544, 551, 643, 982, 1082, 1127, 1241
ApoI RAATTY 3 cut(s) 20, 34, 855
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
Asp718I GGTACC 1 cut(s) 262
AspA2I CCTAGG 1 cut(s) 1225
AspLEI GCGC 4 cut(s) 367, 383, 643, 806
AspS9I GGNCC 2 cut(s) 693, 1222
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 3 cut(s) 437, 740, 1196
AsuNHI GCTAGC 1 cut(s) 1201
AvaII GGWCC 1 cut(s) 693
AvrII CCTAGG 1 cut(s) 1225
AxyI CCTNAGG 1 cut(s) 258
BalI TGGCCA 1 cut(s) 478
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 1 cut(s) 1251
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 1173
BbvI GCAGC 9 cut(s) 387, 390, 538, 556, 630, 969, 1069, 1139, 1228
BccI CCATC 2 cut(s) 470, 1275
BceAI ACGGC 2 cut(s) 473, 576
BciT130I CCWGG 2 cut(s) 743, 1210
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 5 cut(s) 426, 529, 614, 1008, 1048
BfaI CTAG 3 cut(s) 987, 1202, 1226
BfmI CTRYAG 1 cut(s) 1242
BfoI RGCGCY 3 cut(s) 368, 384, 644
BglII AGATCT 1 cut(s) 673
BlnI CCTAGG 1 cut(s) 1225
Bme1390I CCNGG 3 cut(s) 157, 743, 1210
Bme18I GGWCC 1 cut(s) 693
BmgT120I GGNCC 2 cut(s) 693, 1222
BmiI GGNNCC 3 cut(s) 264, 695, 740
BmrFI CCNGG 3 cut(s) 157, 743, 1210
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 1 cut(s) 206
BmtI GCTAGC 1 cut(s) 1205
BmuI ACTGGG 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 558
BpmI CTGGAG 4 cut(s) 662, 764, 1026, 1137
BpuMI CCSGG 1 cut(s) 157
Bsa29I ATCGAT 1 cut(s) 1257
BsaBI GATNNNNATC 1 cut(s) 1191
BsaHI GRCGYC 3 cut(s) 563, 873, 1017
BsaJI CCNNGG 4 cut(s) 155, 768, 1209, 1225
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 667, 868, 1102, 1245
Bse1I ACTGG 3 cut(s) 518, 679, 1120
Bse21I CCTNAGG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 1191
BseBI CCWGG 2 cut(s) 743, 1210
BseCI ATCGAT 1 cut(s) 1257
BseDI CCNNGG 4 cut(s) 155, 768, 1209, 1225
BseJI GATNNNNATC 1 cut(s) 1191
BseLI CCNNNNNNNGG 5 cut(s) 259, 667, 868, 1102, 1245
BseMII CTCAG 5 cut(s) 261, 272, 569, 798, 1114
BseNI ACTGG 3 cut(s) 518, 679, 1120
BseRI GAGGAG 3 cut(s) 761, 851, 977
BseXI GCAGC 9 cut(s) 387, 390, 538, 556, 630, 969, 1069, 1139, 1228
Bsh1236I CGCG 1 cut(s) 779
Bsh1285I CGRYCG 1 cut(s) 1261
BshFI GGCC 4 cut(s) 478, 764, 894, 1223
BshNI GGYRCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 1257
BsiEI CGRYCG 1 cut(s) 1261
BsiHKAI GWGCWC 1 cut(s) 1173
BsiSI CCGG 4 cut(s) 157, 402, 602, 653
BslFI GGGAC 2 cut(s) 160, 812
BslI CCNNNNNNNGG 5 cut(s) 259, 667, 868, 1102, 1245
BsmAI GTCTC 5 cut(s) 426, 529, 614, 1008, 1048
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 2 cut(s) 160, 812
BsnI GGCC 4 cut(s) 478, 764, 894, 1223
Bsp1286I GDGCHC 2 cut(s) 1173, 1251
Bsp143I GATC 3 cut(s) 673, 947, 1258
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 8 cut(s) 147, 195, 498, 536, 710, 765, 926, 1239
BspANI GGCC 4 cut(s) 478, 764, 894, 1223
BspCNI CTCAG 5 cut(s) 262, 271, 568, 799, 1113
BspDI ATCGAT 1 cut(s) 1257
BspFNI CGCG 1 cut(s) 779
BspHI TCATGA 2 cut(s) 1233, 1270
BspLI GGNNCC 3 cut(s) 264, 695, 740
BspMAI CTGCAG 1 cut(s) 1246
BspOI GCTAGC 1 cut(s) 1205
BspT107I GGYRCC 1 cut(s) 262
BsrI ACTGG 3 cut(s) 518, 679, 1120
BssECI CCNNGG 4 cut(s) 155, 768, 1209, 1225
BssMI GATC 3 cut(s) 673, 947, 1258
BssNI GRCGYC 3 cut(s) 563, 873, 1017
BssT1I CCWWGG 2 cut(s) 768, 1225
Bst2UI CCWGG 2 cut(s) 743, 1210
Bst4CI ACNGT 1 cut(s) 1149
BstACI GRCGYC 3 cut(s) 563, 873, 1017
BstC8I GCNNGC 4 cut(s) 507, 549, 1178, 1203
BstDEI CTNAG 6 cut(s) 258, 270, 555, 633, 807, 1100
BstDSI CCRYGG 1 cut(s) 768
BstENI CCTNNNNNAGG 1 cut(s) 257
BstFNI CGCG 1 cut(s) 779
BstH2I RGCGCY 3 cut(s) 368, 384, 644
BstHHI GCGC 4 cut(s) 367, 383, 643, 806
BstKTI GATC 3 cut(s) 676, 950, 1261
BstMAI GTCTC 5 cut(s) 426, 529, 614, 1008, 1048
BstMBI GATC 3 cut(s) 673, 947, 1258
BstMCI CGRYCG 1 cut(s) 1261
BstNI CCWGG 2 cut(s) 743, 1210
BstSCI CCNGG 3 cut(s) 155, 741, 1208
BstSFI CTRYAG 1 cut(s) 1242
BstUI CGCG 1 cut(s) 779
BstV1I GCAGC 9 cut(s) 387, 390, 538, 556, 630, 969, 1069, 1139, 1228
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 1257
Bsu36I CCTNAGG 1 cut(s) 258
BsuRI GGCC 4 cut(s) 478, 764, 894, 1223
BsuTUI ATCGAT 1 cut(s) 1257
BtgI CCRYGG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 1154
Cac8I GCNNGC 4 cut(s) 507, 549, 1178, 1203
CaiI CAGNNNCTG 1 cut(s) 1057
CciI TCATGA 2 cut(s) 1233, 1270
CfoI GCGC 4 cut(s) 367, 383, 643, 806
Cfr13I GGNCC 2 cut(s) 693, 1222
ClaI ATCGAT 1 cut(s) 1257
CseI GACGC 3 cut(s) 552, 862, 1025
Csp6I GTAC 3 cut(s) 263, 296, 658
CviAII CATG 4 cut(s) 624, 769, 1234, 1271
CviQI GTAC 3 cut(s) 263, 296, 658
DdeI CTNAG 6 cut(s) 258, 270, 555, 633, 807, 1100
DpnI GATC 3 cut(s) 675, 949, 1260
DpnII GATC 3 cut(s) 673, 947, 1258
DraIII CACNNNGTG 1 cut(s) 1149
EaeI YGGCCR 1 cut(s) 476
Eco130I CCWWGG 2 cut(s) 768, 1225
Eco24I GRGCYC 1 cut(s) 1251
Eco32I GATATC 1 cut(s) 1193
Eco47I GGWCC 1 cut(s) 693
Eco47III AGCGCT 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 327, 835
Eco81I CCTNAGG 1 cut(s) 258
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 1222
EcoRI GAATTC 1 cut(s) 855
EcoRII CCWGG 2 cut(s) 741, 1208
EcoRV GATATC 1 cut(s) 1193
EcoT14I CCWWGG 2 cut(s) 768, 1225
EcoT38I GRGCYC 1 cut(s) 1251
ErhI CCWWGG 2 cut(s) 768, 1225
Esp3I CGTCTC 1 cut(s) 1008
FaeI CATG 4 cut(s) 627, 772, 1237, 1274
FaqI GGGAC 2 cut(s) 160, 812
FatI CATG 4 cut(s) 623, 768, 1233, 1270
FauI CCCGC 3 cut(s) 154, 717, 919
FriOI GRGCYC 1 cut(s) 1251
FspBI CTAG 3 cut(s) 987, 1202, 1226
GlaI GCGC 4 cut(s) 366, 382, 642, 805
GsuI CTGGAG 4 cut(s) 662, 764, 1026, 1137
HaeII RGCGCY 3 cut(s) 368, 384, 644
HaeIII GGCC 4 cut(s) 478, 764, 894, 1223
HapII CCGG 4 cut(s) 157, 402, 602, 653
HgaI GACGC 3 cut(s) 552, 862, 1025
HhaI GCGC 4 cut(s) 367, 383, 643, 806
Hin1I GRCGYC 3 cut(s) 563, 873, 1017
Hin1II CATG 4 cut(s) 627, 772, 1237, 1274
Hin6I GCGC 4 cut(s) 365, 381, 641, 804
HinP1I GCGC 4 cut(s) 365, 381, 641, 804
HinfI GANTC 7 cut(s) 388, 664, 701, 953, 1031, 1039, 1230
HpaII CCGG 4 cut(s) 157, 402, 602, 653
HphI GGTGA 3 cut(s) 437, 740, 1196
Hpy166II GTNNAC 2 cut(s) 493, 1009
Hpy188III TCNNGA 7 cut(s) 438, 668, 842, 1043, 1196, 1234, 1271
Hpy8I GTNNAC 2 cut(s) 493, 1009
Hpy99I CGWCG 4 cut(s) 488, 586, 878, 908
HpyAV CCTTC 9 cut(s) 20, 73, 145, 263, 309, 631, 859, 1030, 1106
HpyCH4III ACNGT 1 cut(s) 1149
HpyCH4V TGCA 5 cut(s) 596, 646, 1127, 1135, 1244
HpyF3I CTNAG 6 cut(s) 258, 270, 555, 633, 807, 1100
Hsp92I GRCGYC 3 cut(s) 563, 873, 1017
Hsp92II CATG 4 cut(s) 627, 772, 1237, 1274
HspAI GCGC 4 cut(s) 365, 381, 641, 804
KpnI GGTACC 1 cut(s) 266
Kzo9I GATC 3 cut(s) 673, 947, 1258
LmnI GCTCC 4 cut(s) 612, 748, 1168, 1198
Lsp1109I GCAGC 9 cut(s) 387, 390, 538, 556, 630, 969, 1069, 1139, 1228
LweI GCATC 1 cut(s) 206
MaeI CTAG 3 cut(s) 987, 1202, 1226
MaeIII GTNAC 3 cut(s) 842, 989, 1141
MalI GATC 3 cut(s) 675, 949, 1260
MboI GATC 3 cut(s) 673, 947, 1258
MboII GAAGA 9 cut(s) 333, 558, 561, 564, 683, 776, 779, 930, 979
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 2 cut(s) 1173, 1251
MlsI TGGCCA 1 cut(s) 478
MluCI AATT 8 cut(s) 20, 34, 85, 102, 120, 241, 647, 855
MluI ACGCGT 1 cut(s) 777
MluNI TGGCCA 1 cut(s) 478
MlyI GAGTC 2 cut(s) 1033, 1040
MmeI TCCRAC 1 cut(s) 163
Mox20I TGGCCA 1 cut(s) 478
MscI TGGCCA 1 cut(s) 478
MseI TTAA 3 cut(s) 72, 101, 119
Msp20I TGGCCA 1 cut(s) 478
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 4 cut(s) 157, 402, 602, 653
MspR9I CCNGG 3 cut(s) 157, 743, 1210
MunI CAATTG 1 cut(s) 647
MvaI CCWGG 2 cut(s) 743, 1210
MvnI CGCG 1 cut(s) 779
NciI CCSGG 1 cut(s) 157
NcoI CCATGG 1 cut(s) 768
NdeII GATC 3 cut(s) 673, 947, 1258
NheI GCTAGC 1 cut(s) 1201
NlaIII CATG 4 cut(s) 627, 772, 1237, 1274
NlaIV GGNNCC 3 cut(s) 264, 695, 740
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 2 cut(s) 842, 1141
PagI TCATGA 2 cut(s) 1233, 1270
PfeI GAWTC 5 cut(s) 388, 664, 701, 953, 1230
PflFI GACNNNGTC 1 cut(s) 562
Ple19I CGATCG 1 cut(s) 1261
PleI GAGTC 2 cut(s) 1033, 1039
PpsI GAGTC 2 cut(s) 1033, 1039
PsiI TTATAA 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 741, 1208
PspGI CCWGG 2 cut(s) 741, 1208
PspN4I GGNNCC 3 cut(s) 264, 695, 740
PspPI GGNCC 2 cut(s) 693, 1222
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 1 cut(s) 1057
PsuI RGATCY 1 cut(s) 673
PsyI GACNNNGTC 1 cut(s) 562
PvuI CGATCG 1 cut(s) 1261
RsaI GTAC 3 cut(s) 264, 297, 659
RsaNI GTAC 3 cut(s) 263, 296, 658
SaqAI TTAA 3 cut(s) 72, 101, 119
Sau3AI GATC 3 cut(s) 673, 947, 1258
Sau96I GGNCC 2 cut(s) 693, 1222
SchI GAGTC 2 cut(s) 1033, 1040
ScrFI CCNGG 3 cut(s) 157, 743, 1210
SduI GDGCHC 2 cut(s) 1173, 1251
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 1 cut(s) 1242
SinI GGWCC 1 cut(s) 693
Sse9I AATT 8 cut(s) 20, 34, 85, 102, 120, 241, 647, 855
SsiI CCGC 8 cut(s) 147, 195, 498, 536, 710, 765, 926, 1239
SspMI CTAG 3 cut(s) 987, 1202, 1226
StyD4I CCNGG 3 cut(s) 155, 741, 1208
StyI CCWWGG 2 cut(s) 768, 1225
TaaI ACNGT 1 cut(s) 1149
TaqI TCGA 5 cut(s) 699, 1023, 1034, 1257, 1261
TasI AATT 8 cut(s) 20, 34, 85, 102, 120, 241, 647, 855
TauI GCSGC 3 cut(s) 500, 538, 767
TfiI GAWTC 5 cut(s) 388, 664, 701, 953, 1230
Tru1I TTAA 3 cut(s) 72, 101, 119
Tru9I TTAA 3 cut(s) 72, 101, 119
TscAI CASTG 1 cut(s) 1154
TseFI GTSAC 2 cut(s) 842, 1141
TseI GCWGC 9 cut(s) 375, 378, 544, 551, 643, 982, 1082, 1127, 1241
Tsp45I GTSAC 2 cut(s) 842, 1141
TspDTI ATGAA 1 cut(s) 1222
TspGWI ACGGA 3 cut(s) 460, 1026, 1181
TspRI CASTG 1 cut(s) 1154
Tth111I GACNNNGTC 1 cut(s) 562
VpaK11BI GGWCC 1 cut(s) 693
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 3 cut(s) 20, 34, 855
XmaJI CCTAGG 1 cut(s) 1225
XspI CTAG 3 cut(s) 987, 1202, 1226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.