pycom08g20610

Conserved oligomeric Golgi complex subunit 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005295
Physical Location & Seq
Reverse (-)
20327898 .. 20331556
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2394 bp
ATGAGAGTTAATTCCGGCGATGCCCTCAGCGGCGCCGCCACCAGTTCCTTCGCCACTGCAGCCGCCGCTTCTCGAGATGCCGAGTCCCTCTTCCGCTCCAAACCCATTTCCGAGATCCGCACCGTCGAGTCCACCACCCGCACCCAGATCGAGTCCAAGAAGGAGGAGCTCCGTCAACTCGTCGGCACCCGCTACCGGGATCTCATCGACTCAGCCGATTCCATCGTTCTCATGAAGCGCTCCTCCCACTCCATCTCCCAAAACCTCTCCTCCGTCCACGCATCCATCAATTCCCTCTCCTCCGCCTCCTCCGCCGACCCTCCCGATCCCTCCCGCCACGACCCGACCCGCCACCGCATCTACGGCATTGCCTGCCGAGTCAAGTACCTCGTTGACACTCCCGAGAACATCTGGGGCTGCCTCGACGAGTCCATGTTTCTCGAGTCCGCCGCGCGCTACGTCCGCGCCAGCCACGTGCACTCCATCCTCGCCCTCCCCGGTCAGGTGCGCTTCCTCTCCAACTTCCCTCTCCTTCAGCACCAGTGGCAGATCGTCGACAGCTTCAAGTCCCAGATCTCTCAGCGCGCCCGCGACCGCCTCTTCGATCGAGAGCTCCGATTGCCCATCTCCTCCTACGCCGACGCCTTGGCCGCCGTCGCTTTGATCGACGACCTCCGGCCCGAGCACGTCCTCTCCCTCTTCCTCGACAGTCGCAAGTCGTGGGTTTCCGGGATACTCAACGCTTGTCGTCCCGATGCTCAGTGCTCCGACGTCGTTTCAGTGCTATGCGAGGCTCTGAGGGTGATTCAGGTGACTGTGGGGCAGGTTGGGGAGCTCTTCTTGCGCGTGTTGAACGACATGCCGTTGTTCTACAAGGTGGTGTTGGGTTGCCCTCCTGCATCCCAACTGTTCGGTGGAATTCCCAATCCAGATGAGGAGGTCAAGCTTTGGAATTCATTTCGGGAGAAGCTCGAGTCCGCCATGGGAATGCTGGACAAGAATTACATTGCCAAGGCTTGTTGCTCTTGGCTTAACGACTGCGGAGGCCAGATGGTGGATAGGATCAACGGGCGGTTCCTCATCGATGCAATTGGCAGTGGCCATGAGCTCGCCACGGCTGAGAAATTGATTAGGGAGACGATGAACAGCAAAGAGGTATTAGAAGGGAGCTTGGAGTGGCTCAAGAACGTTTTCGGGTCTAACATTGCCCTGCCATGGAGTAGAATGAGTGAACTTGTTCTCGGAGACGATTCAGATCTCTGGGACAGTATCTTCGAACCTGCGTTTGTCGCCAGGATGAAGGTGATTGTGGCATACAGGTTCGAGGATCTCATGAAGGCAGTGAACATTAAGGATGGCGAACCCATTGATTTCCTTGGTGCTGGTGGTGGAGTTTGGTTTGTAGAAACCAAATCGAATAATGCTAAGAAAGGTACTACTAGTGCTTTGCCTTGTGAAGAGAATTGTCTCAATTTCTATTTTGGTCCTCAAGTGAGTGGAATTCGGGATGCCGTGGATAGTAGCTGTAAGGATGTTCTTGATGACATGCTGTGTTTCTTAGAATCGCCAAAGGCAGCTCTTAGGTTAAAGGATCTGGCACCGTATCTACAAGATAAGTGTTGCCTGACCATTTCCAATATATTGGTGCAACTGACTAGTGAGCTTGATAATTTGGAAATTGGCAAAGACACGCAAGGGCCAATAACCGTTGACAGGGCACTTTTCATTGGGAGACTCCTGTTTGCATTGCAGAGCCACTCCAAACACATCCCCATTATTCTTGGCCCTCCAAGGTCTTGGGTAAATGTAACAGGTTCTGTAGTTTTCGATAAGTTCCCAGCCATGTCAAGACAATCTAGAGCTCCCACAGATTCTCCAGTGCTTGATAGCCCCTCGGGTTCTAAAAGGCACACTTCATCTGCTACTGCTGCATTGCTTGGAGCCAGCCAAAGTGCAAGCCCTAAACTTGAAGAGCTTAATTCAACTATGAAAGATCTCCGCATCCGAGCGCATGGTTTGTGGATGTCGTGGTTGTGCGATGAGCTTTCAGTTATTCTTTCCTGTGATCTTCAAAAAGATTATGCTTTGTCATCATCAACACCTCTGAGGGGTTGGGAAGAGACTGTTGTTAAGCAAGAGCAGTCTGATGATAACCAGTCAGACATGAGAATATGGCTCCCATGCATGCCTTCTCTCTATATAGTCTCATTTCTATTTCGTATATGTGAAGAAGTTCATCGAATTGGAGGTCATGTTCTTGACAAAACAATTCTGCAAAAGTTTGCATTAAGACTGCTGGAAAAGGTCATTGGTATTTATGGGGACTTCCTTTCTACAGTAGAGGCTGGTGGATCGATCAAGTGTCAGAGAAAGGAGTTTTGCAAGTTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps51

Protein Analysis

798

Amino Acids

88.43

Weight (kDa)

5.93

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS51_Exo84_N PF08700 24 - 101 1.5e-16 Vps51/EXO84/COG1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 774
Acc36I ACCTGC 2 cut(s) 814, 1288
AccB1I GGYRCC 3 cut(s) 32, 185, 1597
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 555
AccII CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
AclI AACGTT 1 cut(s) 1188
AclWI GGATC 7 cut(s) 109, 207, 320, 1070, 1335, 1599, 2359
AcoI YGGCCR 2 cut(s) 648, 1099
AcsI RAATTY 3 cut(s) 918, 952, 1500
AcuI CTGAAG 1 cut(s) 518
AcvI CACGTG 1 cut(s) 475
AcyI GRCGYC 3 cut(s) 33, 642, 771
AfaI GTAC 2 cut(s) 386, 1435
AfeI AGCGCT 1 cut(s) 239
AfiI CCNNNNNNNGG 7 cut(s) 195, 196, 502, 1054, 1215, 1713, 2108
AgsI TTSAA 5 cut(s) 565, 853, 1970, 1983, 2072
AhlI ACTAGT 2 cut(s) 1439, 1655
AjiI CACGTC 1 cut(s) 688
AjnI CCWGG 1 cut(s) 1292
AjuI GAANNNNNNNTTGG 2 cut(s) 2292, 2324
AloI GAACNNNNNNTCC 2 cut(s) 2238, 2270
Alw21I GWGCWC 8 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1864
Alw26I GTCTC 6 cut(s) 1130, 1239, 1472, 1726, 2114, 2209
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 7 cut(s) 109, 207, 320, 1070, 1335, 1599, 2359
AlwNI CAGNNNCTG 1 cut(s) 1817
Ama87I CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
Aor51HI AGCGCT 1 cut(s) 239
AoxI GGCC 6 cut(s) 648, 677, 1045, 1099, 1697, 1783
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 4 cut(s) 59, 417, 1574, 1928
ApoI RAATTY 3 cut(s) 918, 952, 1500
ArsI GACNNNNNNTTYG 6 cut(s) 584, 616, 1256, 1288, 2233, 2265
Asp700I GAANNNNTTC 3 cut(s) 1190, 1236, 2232
AspS9I GGNCC 4 cut(s) 678, 1484, 1697, 1784
AsuC2I CCSGG 3 cut(s) 197, 498, 730
AsuHPI GGTGA 3 cut(s) 814, 823, 1315
AsuII TTCGAA 1 cut(s) 1275
AvaI CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
AvaII GGWCC 1 cut(s) 1484
BaeGI GKGCMC 2 cut(s) 480, 1720
BalI TGGCCA 1 cut(s) 1101
BanI GGYRCC 3 cut(s) 32, 185, 1597
BanII GRGCYC 5 cut(s) 171, 615, 837, 1110, 1864
BbrPI CACGTG 1 cut(s) 475
Bbv12I GWGCWC 8 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1864
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 4 cut(s) 71, 404, 1586, 1915
BccI CCATC 7 cut(s) 230, 260, 293, 491, 632, 1045, 1349
BceAI ACGGC 5 cut(s) 379, 638, 847, 1131, 1496
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 1294
BciVI GTATCC 1 cut(s) 726
BcnI CCSGG 3 cut(s) 197, 498, 730
BcoDI GTCTC 6 cut(s) 1130, 1239, 1472, 1726, 2114, 2209
BcuI ACTAGT 2 cut(s) 1439, 1655
BfaI CTAG 3 cut(s) 1440, 1656, 1857
BfmI CTRYAG 3 cut(s) 57, 1818, 2334
BfoI RGCGCY 2 cut(s) 36, 241
BfuAI ACCTGC 2 cut(s) 814, 1288
BfuI GTATCC 1 cut(s) 726
BglII AGATCT 3 cut(s) 573, 1255, 1993
Bme1390I CCNGG 4 cut(s) 197, 498, 730, 1294
Bme18I GGWCC 1 cut(s) 1484
BmeT110I CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
BmgBI CACGTC 1 cut(s) 688
BmgT120I GGNCC 4 cut(s) 678, 1484, 1697, 1784
BmiI GGNNCC 6 cut(s) 34, 187, 1076, 1599, 1942, 2177
BmrFI CCNGG 4 cut(s) 197, 498, 730, 1294
BmsI GCATC 9 cut(s) 10, 67, 290, 366, 745, 908, 1075, 1498, 2010
BpmI CTGGAG 1 cut(s) 1860
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 1275
BpuEI CTTGAG 2 cut(s) 1166, 1473
BpuMI CCSGG 3 cut(s) 197, 498, 730
Bsa29I ATCGAT 2 cut(s) 1083, 2354
BsaAI YACGTR 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 1254
BsaHI GRCGYC 3 cut(s) 33, 642, 771
Bsc4I CCNNNNNNNGG 7 cut(s) 195, 196, 502, 1054, 1215, 1713, 2108
Bse1I ACTGG 4 cut(s) 42, 541, 1877, 2155
Bse3DI GCAATG 5 cut(s) 366, 1005, 1203, 1745, 1931
Bse8I GATNNNNATC 1 cut(s) 1254
BseBI CCWGG 1 cut(s) 1294
BseCI ATCGAT 2 cut(s) 1083, 2354
BseJI GATNNNNATC 1 cut(s) 1254
BseLI CCNNNNNNNGG 7 cut(s) 195, 196, 502, 1054, 1215, 1713, 2108
BseMI GCAATG 5 cut(s) 366, 1005, 1203, 1745, 1931
BseMII CTCAG 7 cut(s) 40, 225, 593, 773, 788, 1110, 2096
BseNI ACTGG 4 cut(s) 42, 541, 1877, 2155
BsePI GCGCGC 2 cut(s) 452, 583
BseRI GAGGAG 7 cut(s) 179, 232, 259, 289, 298, 619, 950
BseSI GKGCMC 2 cut(s) 480, 1720
BseXI GCAGC 4 cut(s) 71, 404, 1586, 1915
BseYI CCCAGC 1 cut(s) 1837
Bsh1236I CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
Bsh1285I CGRYCG 2 cut(s) 595, 607
BshFI GGCC 6 cut(s) 650, 679, 1047, 1101, 1699, 1785
BshNI GGYRCC 3 cut(s) 32, 185, 1597
BshVI ATCGAT 2 cut(s) 1083, 2354
BsiEI CGRYCG 2 cut(s) 595, 607
BsiHKAI GWGCWC 8 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1864
BsiHKCI CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
BsiSI CCGG 5 cut(s) 15, 196, 498, 676, 729
BslFI GGGAC 5 cut(s) 70, 553, 735, 1277, 2336
BslI CCNNNNNNNGG 7 cut(s) 195, 196, 502, 1054, 1215, 1713, 2108
BsmAI GTCTC 6 cut(s) 1130, 1239, 1472, 1726, 2114, 2209
BsmBI CGTCTC 2 cut(s) 1130, 1239
BsmFI GGGAC 5 cut(s) 70, 553, 735, 1277, 2336
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 6 cut(s) 650, 679, 1047, 1101, 1699, 1785
BsoBI CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
Bsp119I TTCGAA 1 cut(s) 1275
Bsp1286I GDGCHC 9 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1720, 1864
Bsp19I CCATGG 2 cut(s) 981, 1214
BspANI GGCC 6 cut(s) 650, 679, 1047, 1101, 1699, 1785
BspCNI CTCAG 7 cut(s) 39, 224, 592, 772, 789, 1111, 2097
BspDI ATCGAT 2 cut(s) 1083, 2354
BspFNI CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
BspHI TCATGA 2 cut(s) 231, 1332
BspLI GGNNCC 6 cut(s) 34, 187, 1076, 1599, 1942, 2177
BspMAI CTGCAG 1 cut(s) 61
BspMI ACCTGC 2 cut(s) 814, 1288
BspPI GGATC 7 cut(s) 109, 207, 320, 1070, 1335, 1599, 2359
BspQI GCTCTTC 2 cut(s) 842, 1965
BspT104I TTCGAA 1 cut(s) 1275
BspT107I GGYRCC 3 cut(s) 32, 185, 1597
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 5 cut(s) 366, 1005, 1203, 1745, 1931
BsrI ACTGG 4 cut(s) 42, 541, 1877, 2155
BssHII GCGCGC 2 cut(s) 452, 583
BssNI GRCGYC 3 cut(s) 33, 642, 771
BssT1I CCWWGG 6 cut(s) 645, 981, 1011, 1214, 1375, 1790
Bst2UI CCWGG 1 cut(s) 1294
Bst4CI ACNGT 9 cut(s) 124, 710, 817, 909, 1268, 1602, 1708, 2125, 2338
Bst6I CTCTTC 7 cut(s) 95, 605, 704, 842, 1452, 1965, 2112
BstACI GRCGYC 3 cut(s) 33, 642, 771
BstAPI GCANNNNNTGC 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 1275
BstC8I GCNNGC 9 cut(s) 373, 454, 469, 585, 589, 1110, 1945, 1957, 2186
BstDSI CCRYGG 4 cut(s) 981, 1113, 1214, 1512
BstFNI CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
BstH2I RGCGCY 2 cut(s) 36, 241
BstMAI GTCTC 6 cut(s) 1130, 1239, 1472, 1726, 2114, 2209
BstMCI CGRYCG 2 cut(s) 595, 607
BstNI CCWGG 1 cut(s) 1294
BstNSI RCATGY 3 cut(s) 862, 1549, 2188
BstSCI CCNGG 4 cut(s) 195, 496, 728, 1292
BstSFI CTRYAG 3 cut(s) 57, 1818, 2334
BstSLI GKGCMC 2 cut(s) 480, 1720
BstUI CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
BstV1I GCAGC 4 cut(s) 71, 404, 1586, 1915
BstX2I RGATCY 7 cut(s) 114, 199, 573, 1255, 1327, 1591, 1993
BstXI CCANNNNNNTGG 2 cut(s) 1642, 1797
BstYI RGATCY 7 cut(s) 114, 199, 573, 1255, 1327, 1591, 1993
Bsu15I ATCGAT 2 cut(s) 1083, 2354
BsuI GTATCC 1 cut(s) 726
BsuRI GGCC 6 cut(s) 650, 679, 1047, 1101, 1699, 1785
BsuTUI ATCGAT 2 cut(s) 1083, 2354
BtgI CCRYGG 4 cut(s) 981, 1113, 1214, 1512
BtgZI GCGATG 2 cut(s) 33, 2052
BtrI CACGTC 1 cut(s) 688
BtsI GCAGTG 3 cut(s) 54, 1102, 1347
BtsIMutI CAGTG 7 cut(s) 54, 548, 767, 786, 1102, 1347, 1884
BveI ACCTGC 2 cut(s) 814, 1288
Cac8I GCNNGC 9 cut(s) 373, 454, 469, 585, 589, 1110, 1945, 1957, 2186
CaiI CAGNNNCTG 1 cut(s) 1817
CciI TCATGA 2 cut(s) 231, 1332
Cfr13I GGNCC 4 cut(s) 678, 1484, 1697, 1784
ClaI ATCGAT 2 cut(s) 1083, 2354
CseI GACGC 1 cut(s) 650
Csp6I GTAC 2 cut(s) 385, 1434
CviQI GTAC 2 cut(s) 385, 1434
DinI GGCGCC 1 cut(s) 34
EaeI YGGCCR 2 cut(s) 648, 1099
Eam1104I CTCTTC 7 cut(s) 95, 605, 704, 842, 1452, 1965, 2112
EarI CTCTTC 7 cut(s) 95, 605, 704, 842, 1452, 1965, 2112
EciI GGCGGA 4 cut(s) 292, 301, 436, 967
Ecl136II GAGCTC 5 cut(s) 169, 613, 835, 1108, 1862
Eco130I CCWWGG 6 cut(s) 645, 981, 1011, 1214, 1375, 1790
Eco24I GRGCYC 5 cut(s) 171, 615, 837, 1110, 1864
Eco47I GGWCC 1 cut(s) 1484
Eco47III AGCGCT 1 cut(s) 239
Eco53kI GAGCTC 5 cut(s) 169, 613, 835, 1108, 1862
Eco57I CTGAAG 1 cut(s) 518
Eco72I CACGTG 1 cut(s) 475
Eco88I CYCGRG 6 cut(s) 72, 401, 440, 680, 971, 1894
EcoICRI GAGCTC 5 cut(s) 169, 613, 835, 1108, 1862
EcoRI GAATTC 3 cut(s) 918, 952, 1500
EcoRII CCWGG 1 cut(s) 1292
EcoT14I CCWWGG 6 cut(s) 645, 981, 1011, 1214, 1375, 1790
EcoT22I ATGCAT 1 cut(s) 2186
EcoT38I GRGCYC 5 cut(s) 171, 615, 837, 1110, 1864
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 6 cut(s) 645, 981, 1011, 1214, 1375, 1790
Esp3I CGTCTC 2 cut(s) 1130, 1239
FalI AAGNNNNNCTT 2 cut(s) 1897, 1929
FaqI GGGAC 5 cut(s) 70, 553, 735, 1277, 2336
FauI CCCGC 5 cut(s) 146, 197, 341, 356, 596
FblI GTMKAC 1 cut(s) 555
FriOI GRGCYC 5 cut(s) 171, 615, 837, 1110, 1864
FspBI CTAG 3 cut(s) 1440, 1656, 1857
GsaI CCCAGC 1 cut(s) 1841
GsuI CTGGAG 1 cut(s) 1860
HaeII RGCGCY 2 cut(s) 36, 241
HaeIII GGCC 6 cut(s) 650, 679, 1047, 1101, 1699, 1785
HapII CCGG 5 cut(s) 15, 196, 498, 676, 729
HgaI GACGC 1 cut(s) 650
Hin1I GRCGYC 3 cut(s) 33, 642, 771
HincII GTYRAC 4 cut(s) 176, 394, 556, 1711
HindII GTYRAC 4 cut(s) 176, 394, 556, 1711
HindIII AAGCTT 1 cut(s) 944
HpaII CCGG 5 cut(s) 15, 196, 498, 676, 729
HphI GGTGA 3 cut(s) 814, 823, 1315
Hpy166II GTNNAC 9 cut(s) 132, 176, 277, 394, 478, 556, 1232, 1345, 1711
Hpy8I GTNNAC 9 cut(s) 132, 176, 277, 394, 478, 556, 1232, 1345, 1711
Hpy99I CGWCG 9 cut(s) 128, 185, 428, 557, 644, 659, 671, 773, 776
HpyAV CCTTC 7 cut(s) 58, 154, 542, 1157, 1294, 1330, 2199
HpyCH4III ACNGT 9 cut(s) 124, 710, 817, 909, 1268, 1602, 1708, 2125, 2338
HpyCH4IV ACGT 5 cut(s) 459, 474, 687, 771, 1188
HpySE526I ACGT 5 cut(s) 459, 474, 687, 771, 1188
Hsp92I GRCGYC 3 cut(s) 33, 642, 771
KasI GGCGCC 1 cut(s) 32
LguI GCTCTTC 2 cut(s) 842, 1965
Lsp1109I GCAGC 4 cut(s) 71, 404, 1586, 1915
LweI GCATC 9 cut(s) 10, 67, 290, 366, 745, 908, 1075, 1498, 2010
MaeI CTAG 3 cut(s) 1440, 1656, 1857
MaeII ACGT 5 cut(s) 459, 474, 687, 771, 1188
MaeIII GTNAC 2 cut(s) 811, 1807
MbiI CCGCTC 1 cut(s) 96
MfeI CAATTG 1 cut(s) 1089
MflI RGATCY 7 cut(s) 114, 199, 573, 1255, 1327, 1591, 1993
MhlI GDGCHC 9 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1720, 1864
MlsI TGGCCA 1 cut(s) 1101
MluNI TGGCCA 1 cut(s) 1101
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 9 cut(s) 92, 137, 161, 203, 387, 437, 452, 983, 1728
MmeI TCCRAC 2 cut(s) 543, 792
Mox20I TGGCCA 1 cut(s) 1101
Mph1103I ATGCAT 1 cut(s) 2186
MroXI GAANNNNTTC 3 cut(s) 1190, 1236, 2232
MscI TGGCCA 1 cut(s) 1101
MseI TTAA 7 cut(s) 9, 1032, 1350, 1586, 1977, 2130, 2288
MslI CAYNNNNRTG 1 cut(s) 986
Msp20I TGGCCA 1 cut(s) 1101
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 5 cut(s) 15, 196, 498, 676, 729
MspR9I CCNGG 4 cut(s) 197, 498, 730, 1294
MunI CAATTG 1 cut(s) 1089
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 1 cut(s) 1294
MvnI CGCG 6 cut(s) 452, 454, 465, 585, 591, 846
NarI GGCGCC 1 cut(s) 33
NciI CCSGG 3 cut(s) 197, 498, 730
NcoI CCATGG 2 cut(s) 981, 1214
NlaIV GGNNCC 6 cut(s) 34, 187, 1076, 1599, 1942, 2177
NmeAIII GCCGAG 2 cut(s) 106, 401
NmuCI GTSAC 1 cut(s) 811
NsiI ATGCAT 1 cut(s) 2186
NspI RCATGY 3 cut(s) 862, 1549, 2188
NspV TTCGAA 1 cut(s) 1275
PaeI GCATGC 1 cut(s) 2188
PaeR7I CTCGAG 3 cut(s) 72, 440, 971
PagI TCATGA 2 cut(s) 231, 1332
PauI GCGCGC 2 cut(s) 452, 583
PciSI GCTCTTC 2 cut(s) 842, 1965
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 613, 663
PctI GAATGC 1 cut(s) 993
PdmI GAANNNNTTC 3 cut(s) 1190, 1236, 2232
PfeI GAWTC 5 cut(s) 218, 805, 1250, 1562, 1871
PflMI CCANNNNNTGG 1 cut(s) 1054
PfoI TCCNGGA 1 cut(s) 728
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 9 cut(s) 91, 136, 160, 203, 386, 436, 451, 982, 1728
PluTI GGCGCC 1 cut(s) 36
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
PpsI GAGTC 9 cut(s) 91, 136, 160, 203, 386, 436, 451, 982, 1728
Ppu21I YACGTR 1 cut(s) 475
Psp124BI GAGCTC 5 cut(s) 171, 615, 837, 1110, 1864
Psp1406I AACGTT 1 cut(s) 1188
Psp6I CCWGG 1 cut(s) 1292
PspCI CACGTG 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 1837
PspGI CCWGG 1 cut(s) 1292
PspN4I GGNNCC 6 cut(s) 34, 187, 1076, 1599, 1942, 2177
PspPI GGNCC 4 cut(s) 678, 1484, 1697, 1784
PspXI VCTCGAGB 1 cut(s) 971
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 1817
PsuI RGATCY 7 cut(s) 114, 199, 573, 1255, 1327, 1591, 1993
PteI GCGCGC 2 cut(s) 452, 583
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 2 cut(s) 386, 1435
RsaNI GTAC 2 cut(s) 385, 1434
RseI CAYNNNNRTG 1 cut(s) 986
SacI GAGCTC 5 cut(s) 171, 615, 837, 1110, 1864
SalI GTCGAC 1 cut(s) 554
SapI GCTCTTC 2 cut(s) 842, 1965
SaqAI TTAA 7 cut(s) 9, 1032, 1350, 1586, 1977, 2130, 2288
Sau96I GGNCC 4 cut(s) 678, 1484, 1697, 1784
SchI GAGTC 9 cut(s) 92, 137, 161, 203, 387, 437, 452, 983, 1728
ScrFI CCNGG 4 cut(s) 197, 498, 730, 1294
SduI GDGCHC 9 cut(s) 171, 480, 615, 687, 767, 837, 1110, 1720, 1864
SfaNI GCATC 9 cut(s) 10, 67, 290, 366, 745, 908, 1075, 1498, 2010
SfcI CTRYAG 3 cut(s) 57, 1818, 2334
SfoI GGCGCC 1 cut(s) 34
Sfr274I CTCGAG 3 cut(s) 72, 440, 971
SfuI TTCGAA 1 cut(s) 1275
SinI GGWCC 1 cut(s) 1484
SlaI CTCGAG 3 cut(s) 72, 440, 971
SmiMI CAYNNNNRTG 1 cut(s) 986
SmlI CTYRAG 5 cut(s) 72, 440, 971, 1181, 1488
SmoI CTYRAG 5 cut(s) 72, 440, 971, 1181, 1488
SpeI ACTAGT 2 cut(s) 1439, 1655
SphI GCATGC 1 cut(s) 2188
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 1440, 1656, 1857
SstI GAGCTC 5 cut(s) 171, 615, 837, 1110, 1864
StyD4I CCNGG 4 cut(s) 195, 496, 728, 1292
StyI CCWWGG 6 cut(s) 645, 981, 1011, 1214, 1375, 1790
TaaI ACNGT 9 cut(s) 124, 710, 817, 909, 1268, 1602, 1708, 2125, 2338
TaiI ACGT 5 cut(s) 462, 477, 690, 774, 1191
TauI GCSGC 6 cut(s) 33, 38, 65, 68, 452, 653
TfiI GAWTC 5 cut(s) 218, 805, 1250, 1562, 1871
Tru1I TTAA 7 cut(s) 9, 1032, 1350, 1586, 1977, 2130, 2288
Tru9I TTAA 7 cut(s) 9, 1032, 1350, 1586, 1977, 2130, 2288
TscAI CASTG 7 cut(s) 61, 548, 767, 786, 1102, 1347, 1884
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 4 cut(s) 59, 417, 1574, 1928
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 9 cut(s) 248, 945, 1157, 1313, 1349, 1714, 1905, 2003, 2225
TspGWI ACGGA 2 cut(s) 161, 262
TspRI CASTG 7 cut(s) 61, 548, 767, 786, 1102, 1347, 1884
Van91I CCANNNNNTGG 1 cut(s) 1054
VneI GTGCAC 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 1484
XapI RAATTY 3 cut(s) 918, 952, 1500
XbaI TCTAGA 1 cut(s) 1856
XceI RCATGY 3 cut(s) 862, 1549, 2188
XcmI CCANNNNNNNNNTGG 2 cut(s) 911, 988
XhoI CTCGAG 3 cut(s) 72, 440, 971
XmiI GTMKAC 1 cut(s) 555
XmnI GAANNNNTTC 3 cut(s) 1190, 1236, 2232
XspI CTAG 3 cut(s) 1440, 1656, 1857
ZraI GACGTC 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 2186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.