pycom09g01460

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013512
Physical Location & Seq
Reverse (-)
1148390 .. 1151071
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1755 bp
ATGCAATCTTTGAAGCTGTTTCTTTTGTTGCAATTGTATCATAGCTTTATCTTTTATAAAACCAAAATCATTGTATTGTTTTGTTTTGTGTGTGCTTGTTTTTTGGATTGCAGCAAGAGGCTGATCAAAGAGGTGCACTGCCGGCTCAAGTTGTTGAAAAACAAGAGGCAAGCAATTGTCAGGCAACTACGTCAAGACGTGGCGGAGCTGATCAAACACGGTCACGAAGACAAAGCCTTCAACAGGGTTGAGCAGATAATCAAAGACGAATCCGTAGTGGCATTATATGAAGTGTTGGACAATTTCTGTGAATTCATCCTCATCCACCTTCCTTACATCCGCAGACACAAGGACTGCCCAAATGACATAAATGAAGCAGTTTCAAGTCTTATATATGCTTCTGCAAGATGTGGGGATCTGCCTGAGCTTAGGGGGATCAGGAAGCTATTCGGAGAACGTTACGGGCAGAAATTCACAATGAGTGCTCTTGAACTACTCCCTGGGAATCTGGTGAGCCGTGAGGTAATAGAGAAACTCTCCCAAAAGTCAGTAACAGATGATATGAAGCATATATTGGTCAGTGAAATAGCAAGAAATTACTGCATCAAACCACAGGTTTTAGCTATTGAGTACTATTCAGAATGGCAACAAAAGGTGAAGGAAATTAGTGGACATCAAGTACTGGATGCAGATTATTATGGACGAACCAAAACATCTGGAATGCAAGTTTTAAACGTCGAGGAAATGGAAAGAGAAGCCGTAGATGCTGATTCTAATGTATCTAGAACTTCTGCTAGTTCTTCATTAGTTATGCCACCGATTGAAGGGAATTTTTCTGAAGCTGGTTCTCCAAATATATCAGCTCCGGGCACCGAAGAAAGTCGGTGCAGTACTTCTGGTAGCACAAGTCATGGTACAACAAGAGATGGAGATATTGTGATTTACCTTGATGACATAGAAGAGGTTCAGTTTTCTACAACAAGAGATGCAGACTCCCAGGACCAAAGACTCTTCAAGTTCAAAGGTATCGCACCGAAGAGGGAGAATCTTGAAAGCAGTTGTGATCTCAGTTATGTGGAATGTTATGAGACTTGGAGTGAGAATTGCGAGTCAAAAAGCCCAAGGAGATCAAGGTGGAAGGGAAGTGGGAGAAGACCGAGGAAGAGATCGATTTCTCAGGAAAATCAATGCTTTAAGGATAATGAATGTCTAAATTACTATGGCAGAAAGCATCAAGAAGTTCAACCGTGGAAGCAAAAGAGAGGGATTACAGCTAGGGTTGGATTTCCAACTTATGGACATGAACAATTGAACAGGGGAATCGAATGGCCTGCAGTGCGTCCAGAGCCAATGAGGAGGAAGAGCTATGGCAATGAAGCAATGATGTATAACGTTTTCACGTATCCAGATCATCAGCATCCCACTATGCAAAACAAAGTGCTCAAAGGAAGAGTAGAGGAATCGAGTTTTCGATGCAGGCGTGCCTCATATAATTCCACCTCTCCACATGTGACTAATTCTTGGAGTGCAAGAAAAGGAACACTACCTCCTAATTCCAGAGCCGTGACAATGCCGCCCGAAAGAGCTAAGGACAATCACAAAGATGACTTCCGACGATCGTATTCCGATGCCTTCCAGTATCCTACTCATGTCCACCCTAAGCTGCCTGACTATGATGATATAGCAGCTAAATTCATGGCACTGAAGAAAGAGCGTCTGCAAAATAAGCATCGTTGCAACAACCAGAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

585

Amino Acids

67.56

Weight (kDa)

8.97

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 39 - 202 5e-53 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013512)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G14830
fragaria_vesca FvH4_6g45140
malus_domestica MD09G1088800.v1.1 MD17G1078700.v1.1
prunus_persica Prupe.3G236100_v2.0.a1 Prupe.3G236100_v2.0.a1 Prupe.5G187400_v2.0.a1
pyrus_communis pycom09g01460
rosa_chinensis RchiOBHm_Chr2g0162561
rosa_laevigata RLG00000021352
rosa_roxburghii Rroxscaffold_2G00087830
rosa_rugosa Rorug02G0498400
rosa_samantha Rh2AG564400 Rh2BG577000 Rh2BG577100 Rh2CG547000 Rh2DG586900
rosa_wichuraiana Rw2G046690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 1295
AciI CCGC 3 cut(s) 203, 340, 1574
AclI AACGTT 2 cut(s) 457, 1392
AclWI GGATC 2 cut(s) 423, 443
AcsI RAATTY 5 cut(s) 311, 470, 829, 1691, 1749
AcuI CTGAAG 2 cut(s) 858, 1724
AfaI GTAC 4 cut(s) 632, 681, 892, 916
AfiI CCNNNNNNNGG 3 cut(s) 243, 824, 1295
AflIII ACRYGT 1 cut(s) 1507
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 499, 996
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AloI GAACNNNNNNTCC 2 cut(s) 1531, 1563
Alw21I GWGCWC 3 cut(s) 138, 487, 1443
Alw26I GTCTC 1 cut(s) 1082
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 2 cut(s) 423, 443
AoxI GGCC 1 cut(s) 1328
ApaLI GTGCAC 1 cut(s) 134
ApeKI GCWGC 3 cut(s) 111, 1663, 1685
ApoI RAATTY 5 cut(s) 311, 470, 829, 1691, 1749
Asp700I GAANNNNTTC 2 cut(s) 446, 963
AspS9I GGNCC 1 cut(s) 1000
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 2 cut(s) 523, 667
AvaII GGWCC 1 cut(s) 1000
BaeGI GKGCMC 2 cut(s) 138, 872
BanI GGYRCC 1 cut(s) 869
BbsI GAAGAC 2 cut(s) 234, 1159
Bbv12I GWGCWC 3 cut(s) 138, 487, 1443
BbvI GCAGC 3 cut(s) 123, 1650, 1697
BccI CCATC 1 cut(s) 920
BceAI ACGGC 3 cut(s) 501, 743, 1547
BcgI CGANNNNNNTGC 2 cut(s) 1313, 1347
BciT130I CCWGG 2 cut(s) 501, 998
BciVI GTATCC 2 cut(s) 1413, 1650
BclI TGATCA 2 cut(s) 123, 210
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 1 cut(s) 1082
BfaI CTAG 3 cut(s) 783, 795, 1275
BfmI CTRYAG 1 cut(s) 1332
BfuI GTATCC 2 cut(s) 1413, 1650
BisI GCNGC 4 cut(s) 112, 1574, 1664, 1686
BlsI GCNGC 4 cut(s) 113, 1575, 1665, 1687
BmcAI AGTACT 3 cut(s) 632, 681, 892
Bme1390I CCNGG 3 cut(s) 501, 867, 998
Bme18I GGWCC 1 cut(s) 1000
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 1000
BmiI GGNNCC 1 cut(s) 871
BmrFI CCNGG 3 cut(s) 501, 867, 998
BmsI GCATC 9 cut(s) 612, 676, 754, 976, 1240, 1426, 1463, 1618, 1738
BpiI GAAGAC 2 cut(s) 234, 1159
BplI GAGNNNNNCTC 2 cut(s) 521, 553
Bpu10I CCTNAGC 4 cut(s) 423, 428, 1587, 1659
BpuEI CTTGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 867
Bsa29I ATCGAT 1 cut(s) 1169
BsaAI YACGTR 1 cut(s) 1401
BsaJI CCNNGG 6 cut(s) 499, 500, 996, 1121, 1157, 1247
BsaXI ACNNNNNCTCC 4 cut(s) 921, 951, 1531, 1561
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 824, 1295
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 687, 1636
Bse3DI GCAATG 2 cut(s) 1378, 1386
BseBI CCWGG 2 cut(s) 501, 998
BseCI ATCGAT 1 cut(s) 1169
BseDI CCNNGG 6 cut(s) 499, 500, 996, 1121, 1157, 1247
BseGI GGATG 5 cut(s) 315, 321, 336, 691, 1417
BseLI CCNNNNNNNGG 3 cut(s) 243, 824, 1295
BseMI GCAATG 2 cut(s) 1378, 1386
BseMII CTCAG 3 cut(s) 414, 1081, 1190
BseNI ACTGG 2 cut(s) 687, 1636
BseRI GAGGAG 1 cut(s) 1369
BseSI GKGCMC 2 cut(s) 138, 872
BseXI GCAGC 3 cut(s) 123, 1650, 1697
BsgI GTGCAG 1 cut(s) 907
Bsh1285I CGRYCG 1 cut(s) 1619
BshFI GGCC 1 cut(s) 1330
BshNI GGYRCC 1 cut(s) 869
BshVI ATCGAT 1 cut(s) 1169
BsiEI CGRYCG 1 cut(s) 1619
BsiHKAI GWGCWC 3 cut(s) 138, 487, 1443
BsiSI CCGG 2 cut(s) 142, 866
BslI CCNNNNNNNGG 3 cut(s) 243, 824, 1295
BsmAI GTCTC 1 cut(s) 1082
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 1 cut(s) 1330
Bsp1286I GDGCHC 4 cut(s) 138, 487, 872, 1443
Bsp143I GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
BspACI CCGC 3 cut(s) 203, 340, 1574
BspANI GGCC 1 cut(s) 1330
BspCNI CTCAG 3 cut(s) 415, 1080, 1189
BspDI ATCGAT 1 cut(s) 1169
BspLI GGNNCC 1 cut(s) 871
BspMAI CTGCAG 1 cut(s) 1336
BspPI GGATC 2 cut(s) 423, 443
BspQI GCTCTTC 1 cut(s) 1355
BspT107I GGYRCC 1 cut(s) 869
BsrDI GCAATG 2 cut(s) 1378, 1386
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 2 cut(s) 687, 1636
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 6 cut(s) 499, 500, 996, 1121, 1157, 1247
BssMI GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 2 cut(s) 501, 998
Bst4CI ACNGT 2 cut(s) 221, 1248
Bst6I CTCTTC 6 cut(s) 954, 1016, 1031, 1157, 1355, 1444
BstBAI YACGTR 1 cut(s) 1401
BstC8I GCNNGC 5 cut(s) 143, 171, 1332, 1478, 1482
BstDEI CTNAG 6 cut(s) 423, 428, 1067, 1176, 1587, 1659
BstDSI CCRYGG 1 cut(s) 1247
BstENI CCTNNNNNAGG 1 cut(s) 241
BstF5I GGATG 5 cut(s) 315, 321, 336, 691, 1417
BstKTI GATC 9 cut(s) 126, 213, 418, 438, 1066, 1130, 1169, 1411, 1619
BstMAI GTCTC 1 cut(s) 1082
BstMBI GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
BstMCI CGRYCG 1 cut(s) 1619
BstMWI GCNNNNNNNGC 5 cut(s) 142, 764, 1336, 1345, 1726
BstNI CCWGG 2 cut(s) 501, 998
BstNSI RCATGY 1 cut(s) 1511
BstSCI CCNGG 3 cut(s) 499, 865, 996
BstSFI CTRYAG 1 cut(s) 1332
BstSLI GKGCMC 2 cut(s) 138, 872
BstV1I GCAGC 3 cut(s) 123, 1650, 1697
BstV2I GAAGAC 2 cut(s) 234, 1159
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 1169
BsuI GTATCC 2 cut(s) 1413, 1650
BsuRI GGCC 1 cut(s) 1330
BsuTUI ATCGAT 1 cut(s) 1169
BtgI CCRYGG 1 cut(s) 1247
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 5 cut(s) 315, 321, 336, 691, 1417
BtsI GCAGTG 2 cut(s) 136, 1341
BtsIMutI CAGTG 4 cut(s) 136, 586, 1341, 1700
Cac8I GCNNGC 5 cut(s) 143, 171, 1332, 1478, 1482
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 1000
ClaI ATCGAT 1 cut(s) 1169
CseI GACGC 2 cut(s) 1328, 1703
Csp6I GTAC 4 cut(s) 631, 680, 891, 915
CviAII CATG 5 cut(s) 911, 1301, 1508, 1649, 1696
CviQI GTAC 4 cut(s) 631, 680, 891, 915
DdeI CTNAG 6 cut(s) 423, 428, 1067, 1176, 1587, 1659
DpnI GATC 9 cut(s) 125, 212, 417, 437, 1065, 1129, 1168, 1410, 1618
DpnII GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
DraI TTTAAA 1 cut(s) 732
Eam1104I CTCTTC 6 cut(s) 954, 1016, 1031, 1157, 1355, 1444
EarI CTCTTC 6 cut(s) 954, 1016, 1031, 1157, 1355, 1444
EciI GGCGGA 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 1121
Eco47I GGWCC 1 cut(s) 1000
Eco57I CTGAAG 2 cut(s) 858, 1724
EcoNI CCTNNNNNAGG 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 499, 996
EcoT14I CCWWGG 1 cut(s) 1121
ErhI CCWWGG 1 cut(s) 1121
FaeI CATG 5 cut(s) 914, 1304, 1511, 1652, 1699
FatI CATG 5 cut(s) 910, 1300, 1507, 1648, 1695
FbaI TGATCA 2 cut(s) 123, 210
Fnu4HI GCNGC 4 cut(s) 112, 1574, 1664, 1686
FokI GGATG 5 cut(s) 302, 308, 323, 698, 1404
Fsp4HI GCNGC 4 cut(s) 112, 1574, 1664, 1686
FspBI CTAG 3 cut(s) 783, 795, 1275
GluI GCNGC 4 cut(s) 112, 1574, 1664, 1686
HaeIII GGCC 1 cut(s) 1330
HapII CCGG 2 cut(s) 142, 866
HgaI GACGC 2 cut(s) 1328, 1703
Hin1II CATG 5 cut(s) 914, 1304, 1511, 1652, 1699
HinfI GANTC 9 cut(s) 269, 505, 770, 992, 1008, 1045, 1109, 1320, 1460
HpaII CCGG 2 cut(s) 142, 866
HphI GGTGA 2 cut(s) 523, 667
Hpy166II GTNNAC 3 cut(s) 136, 671, 1654
Hpy188I TCNGA 5 cut(s) 452, 640, 838, 1613, 1627
Hpy8I GTNNAC 3 cut(s) 136, 671, 1654
Hpy99I CGWCG 2 cut(s) 740, 1617
HpyAV CCTTC 6 cut(s) 247, 338, 652, 818, 1132, 1642
HpyCH4III ACNGT 2 cut(s) 221, 1248
HpyCH4IV ACGT 6 cut(s) 190, 198, 457, 735, 1392, 1400
HpyF10VI GCNNNNNNNGC 5 cut(s) 142, 764, 1336, 1345, 1726
HpyF3I CTNAG 6 cut(s) 423, 428, 1067, 1176, 1587, 1659
HpySE526I ACGT 6 cut(s) 190, 198, 457, 735, 1392, 1400
Hsp92II CATG 5 cut(s) 914, 1304, 1511, 1652, 1699
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Ksp22I TGATCA 2 cut(s) 123, 210
Kzo9I GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
LguI GCTCTTC 1 cut(s) 1355
LmnI GCTCC 2 cut(s) 205, 868
Lsp1109I GCAGC 3 cut(s) 123, 1650, 1697
LweI GCATC 9 cut(s) 612, 676, 754, 976, 1240, 1426, 1463, 1618, 1738
MaeI CTAG 3 cut(s) 783, 795, 1275
MaeII ACGT 6 cut(s) 190, 198, 457, 735, 1392, 1400
MaeIII GTNAC 5 cut(s) 221, 458, 550, 1510, 1564
MalI GATC 9 cut(s) 125, 212, 417, 437, 1065, 1129, 1168, 1410, 1618
MboI GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
MfeI CAATTG 3 cut(s) 32, 174, 1307
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 4 cut(s) 138, 487, 872, 1443
MlyI GAGTC 3 cut(s) 986, 1002, 1118
MmeI TCCRAC 4 cut(s) 276, 1261, 1313, 1636
MroNI GCCGGC 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 446, 963
MseI TTAA 2 cut(s) 731, 1194
MslI CAYNNNNRTG 1 cut(s) 1602
MspI CCGG 2 cut(s) 142, 866
MspR9I CCNGG 3 cut(s) 501, 867, 998
MunI CAATTG 3 cut(s) 32, 174, 1307
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 2 cut(s) 501, 998
MwoI GCNNNNNNNGC 5 cut(s) 142, 764, 1336, 1345, 1726
NaeI GCCGGC 1 cut(s) 143
NciI CCSGG 1 cut(s) 867
NdeII GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 5 cut(s) 914, 1304, 1511, 1652, 1699
NlaIV GGNNCC 1 cut(s) 871
NmuCI GTSAC 3 cut(s) 221, 1510, 1564
NspI RCATGY 1 cut(s) 1511
PasI CCCWGGG 1 cut(s) 500
PciI ACATGT 1 cut(s) 1507
PciSI GCTCTTC 1 cut(s) 1355
PcsI WCGNNNNNNNCGW 1 cut(s) 1477
PctI GAATGC 1 cut(s) 726
PdiI GCCGGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 446, 963
PfeI GAWTC 6 cut(s) 269, 505, 770, 1045, 1320, 1460
PflMI CCANNNNNTGG 1 cut(s) 1295
PkrI GCNGC 4 cut(s) 113, 1575, 1665, 1687
Ple19I CGATCG 1 cut(s) 1619
PleI GAGTC 3 cut(s) 986, 1002, 1117
PpsI GAGTC 3 cut(s) 986, 1002, 1117
Ppu21I YACGTR 1 cut(s) 1401
PscI ACATGT 1 cut(s) 1507
PsiI TTATAA 1 cut(s) 57
Psp1406I AACGTT 2 cut(s) 457, 1392
Psp6I CCWGG 2 cut(s) 499, 996
PspGI CCWGG 2 cut(s) 499, 996
PspN4I GGNNCC 1 cut(s) 871
PspPI GGNCC 1 cut(s) 1000
PstI CTGCAG 1 cut(s) 1336
PsuI RGATCY 1 cut(s) 415
PvuI CGATCG 1 cut(s) 1619
RsaI GTAC 4 cut(s) 632, 681, 892, 916
RsaNI GTAC 4 cut(s) 631, 680, 891, 915
RseI CAYNNNNRTG 1 cut(s) 1602
SapI GCTCTTC 1 cut(s) 1355
SaqAI TTAA 2 cut(s) 731, 1194
SatI GCNGC 4 cut(s) 112, 1574, 1664, 1686
Sau3AI GATC 9 cut(s) 123, 210, 415, 435, 1063, 1127, 1166, 1408, 1616
Sau96I GGNCC 1 cut(s) 1000
ScaI AGTACT 3 cut(s) 632, 681, 892
SchI GAGTC 3 cut(s) 986, 1002, 1118
ScrFI CCNGG 3 cut(s) 501, 867, 998
SduI GDGCHC 4 cut(s) 138, 487, 872, 1443
SfaNI GCATC 9 cut(s) 612, 676, 754, 976, 1240, 1426, 1463, 1618, 1738
SfcI CTRYAG 1 cut(s) 1332
SinI GGWCC 1 cut(s) 1000
SmiMI CAYNNNNRTG 1 cut(s) 1602
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SsiI CCGC 3 cut(s) 203, 340, 1574
SspMI CTAG 3 cut(s) 783, 795, 1275
StyD4I CCNGG 3 cut(s) 499, 865, 996
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 2 cut(s) 221, 1248
TaiI ACGT 6 cut(s) 193, 201, 460, 738, 1395, 1403
TaqI TCGA 5 cut(s) 738, 1169, 1323, 1463, 1471
TaqII GACCGA 1 cut(s) 1171
TatI WGTACW 3 cut(s) 630, 679, 890
TauI GCSGC 1 cut(s) 1576
TfiI GAWTC 6 cut(s) 269, 505, 770, 1045, 1320, 1460
Tru1I TTAA 2 cut(s) 731, 1194
Tru9I TTAA 2 cut(s) 731, 1194
TscAI CASTG 4 cut(s) 143, 586, 1341, 1707
TseFI GTSAC 3 cut(s) 221, 1510, 1564
TseI GCWGC 3 cut(s) 111, 1663, 1685
Tsp45I GTSAC 3 cut(s) 221, 1510, 1564
TspDTI ATGAA 9 cut(s) 303, 304, 387, 578, 792, 1218, 1317, 1389, 1684
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 4 cut(s) 143, 586, 1341, 1707
Van91I CCANNNNNTGG 1 cut(s) 1295
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 1000
XagI CCTNNNNNAGG 1 cut(s) 241
XapI RAATTY 5 cut(s) 311, 470, 829, 1691, 1749
XbaI TCTAGA 1 cut(s) 782
XceI RCATGY 1 cut(s) 1511
XmnI GAANNNNTTC 2 cut(s) 446, 963
XspI CTAG 3 cut(s) 783, 795, 1275
ZrmI AGTACT 3 cut(s) 632, 681, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.