Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0006169

Physical Location & Seq

Genomic Coordinates Reverse (-)

1731113 .. 1733178

Gene Structure 2066 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGGATACCAAAACCAAGAAAAGATTGGTGCTTTCAGTTGTTTTCTTCTGCTTCCTCAAAACCCATGTCTCCCTTGCAGCCGACTCCATCACCGCCAACCAATCTCTCTCCGGTGATCAAACCATTGTCTCCGCCGGTGGTGTTTTCGAGCTCGGTTTCTTCGCACCAGGTAATTCTTCAAACATCTACATCGGCATATGGTACACCAAACAACTAGTATCCGAGAGGACCATAGTTTGGGTGGCAAACAGAGAAAAACCTGTTTCCGATAGATTTTCTTCGGTTTTAAGGATTTCAGATGGTAATTTAGTTCTCTTTAACGAGTCCGAAACCCCCGTTTGGTCAACGAATTTAACCTCCTCCTCCGCCGCCACTGCACTTCTTTTGGATGCCGGAAACCTGGTCTTGAGAGAGGGGTCTAGTAATAGTACGTCAGAGCCGTTATGGCAAAGCTTTGATCATCCGGCTCATACATGGCTGCCAGGAGCTAGAATTGGGTTTAACGCTGTTACCAAACAAACCCAAATTCTTACTTCATGGAAGAGTTCGGAGGATCCTGCACCGGGTCTTTTCTCGCTCGAGTTAGACCCGAACGGGAGCGATGCGTATTTCTTACGGTGGAATAGGTCTAGGCAGTATTGGAGTAGTGGACCATGGGATGCAAAATCAAAGCTTTTCAGCTGGGTTCCGGAAATGAGGCTCAATTATATATACAACTTCAGCTTTGTTACGAACGCAAACGAGAGTTACTTCACCTATTCTCTTTATGATCCAAAGATAATTTCTCGATTCCTGATGGATACTTCGGGGCAGATTCAACAGCTGACATGGTTGGAGCCTTCCAGGCAATGGAATATGTTTTGGTCTCAACCAAGACAACGATGCGAAGTTTATGATTTGTGTGGCGCCTTCGGAACTTGCAATCAGGAAAGCGCCCTCTCCTGTAATTGCTTGAACGGTTTTGAGCCAAAGGCGAAGAGAGATTGGGATTTGCAGGACTACTCTGGTGGGTGTAAGAGAAAAACCGATCTGCATTGTGAAAATGCTACTAGCGCGAACAGGAAGCCAGACCGGTTTCTTGAACTTCCTAGCATGTCATTGCCTGAAAATGAACAATCTGTGGAGGTTGGGAATATTGCGGAATGTGAATCGTTCTGCCTTAGAAACTGCTCTTGCACTGCTTATTCTTATAACACAAGTGGATGTTCGATTTGGGAAGGAGAGCTCTTGAATTTGCAACAACTAACATCAAGTGTCGACCAGGGAATAACTTTATACCTCAGACTTGCAGCTTCTGAGTTTAAGAATTCTAAAAGTAACAAGGGATTGATTGTTGGTGTTGTGGCAGGCTCAGCTGCCGGCTTAGCAGTCCTTTTAGGCCTTATTGTGTTTGTAATCTTGGGACAGAGAAAGCGAGGGACTGGAATGGGAAAAGCAGTAGAGGGTTCGTTGATGGCGTTCGGGTACAGAGAGATGCAAGATGCAACGAAAAACTTCTCTGAAAAATTGGGGGGAGGAGGTTTTGGTTCTGTTTTCAAAGGGACATTGCCTGATTCATCTGTCATAGCAGTGAAGAAGCTTGAAAGTGTTAGCCAAGGGGAAAAGCAATTCAGAACAGAAGTGAGCACAATTGGGACAATCCAACATGTCAACCTTGTCCGCCTTCGCGGGTTCTGCTCTGAAGGTGCTAAAAGGATGTTGGTTTATGATTACATGCCGAATGGGTCTTTAGACTCTCACCTTTTCAACGATCAGCATCCGAATGTCTTGAGTTGGAAAACAAGGTACCAAATTGCACTTGGGACAGCAAGAGGGTTGGCTTATCTTCACGAGAAATGCAGAGATTGCATCATACATTGTGACATAAAGCCGGAAAACATTCTCTTGGACGCTGAACTTTGCCCCAAAGTGGCAGACTTTGGCCTTGCAAAGCTTGTTGGAAGAGAGTTCATGGATTTCGGGCGTGGCGATCACAGTGAAAGCAGATGTGTACAGCTACGGAATGATGCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase S_locus_glycop

Protein Analysis

672

Amino Acids

74.63

Weight (kDa)

6.8

Isoelectric Point (pI)

37.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	77 - 181	7.2e-33	D-mannose binding lectin
S_locus_glycop	PF00954	214 - 324	5e-32	S-locus glycoprotein domain
PAN_2	PF08276	354 - 411	6.8e-16	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	499 - 646	1.5e-29	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	500 - 648	3.5e-30	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19130
fragaria_vesca	FvH4_6g44310
malus_domestica	MD09G1098900.v1.1 MD17G1087200.v1.1
prunus_persica	Prupe.3G227300_v2.0.a1 Prupe.3G227500_v2.0.a1
pyrus_communis	pycom09g02260 pycom17g08440
rosa_chinensis	RchiOBHm_Chr2g0161171 RchiOBHm_Chr2g0161191
rosa_laevigata	RLG00000021264 RLG00000021266 RLG00000029382
rosa_multiflora	Rmu_sc0006420.1_g000040 Rmu_sc0006420.1_g000045
rosa_roxburghii	Rroxscaffold_2G00089040 Rroxscaffold_2G00089060
rosa_rugosa	Rorug02G0486800 Rorug02G0486900
rosa_samantha	Rh2AG553500 Rh2BG567200 Rh2CG537300 Rh2CG537500 Rh2DG576500 Rh2DG576600
rosa_wichuraiana	Rw2G045780 Rw2G045800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1191
Acc65I	GGTACC	1 cut(s)	1785
AccB1I	GGYRCC	2 cut(s)	905, 1785
AccB7I	CCANNNNNTGG	2 cut(s)	237, 849
AccI	GTMKAC	1 cut(s)	1257
AccII	CGCG	2 cut(s)	1055, 1668
AccIII	TCCGGA	1 cut(s)	688
AciI	CCGC	7 cut(s)	93, 132, 366, 369, 1139, 1660, 1668
AclWI	GGATC	3 cut(s)	548, 561, 764
AcsI	RAATTY	4 cut(s)	349, 525, 1231, 1306
AcuI	CTGAAG	2 cut(s)	703, 1701
AcyI	GRCGYC	1 cut(s)	906
AfaI	GTAC	5 cut(s)	203, 430, 1466, 1787, 1992
AfiI	CCNNNNNNNGG	5 cut(s)	237, 339, 563, 849, 1909
AflIII	ACRYGT	1 cut(s)	1645
AgeI	ACCGGT	1 cut(s)	1071
AgsI	TTSAA	8 cut(s)	180, 818, 955, 1082, 1231, 1537, 1583, 1747
AhlI	ACTAGT	1 cut(s)	214
AjnI	CCWGG	5 cut(s)	166, 399, 481, 842, 1260
AjuI	GAANNNNNNNTTGG	2 cut(s)	766, 798
Alw21I	GWGCWC	3 cut(s)	153, 1227, 1628
Alw26I	GTCTC	3 cut(s)	73, 133, 870
AlwI	GGATC	3 cut(s)	548, 561, 764
AlwNI	CAGNNNCTG	1 cut(s)	1295
Ama87I	CYCGRG	1 cut(s)	578
Aor13HI	TCCGGA	1 cut(s)	688
AoxI	GGCC	2 cut(s)	1378, 1921
ApeKI	GCWGC	4 cut(s)	77, 478, 1289, 1355
ApoI	RAATTY	4 cut(s)	349, 525, 1231, 1306
ArsI	GACNNNNNNTTYG	2 cut(s)	51, 83
AsiGI	ACCGGT	1 cut(s)	1071
Asp700I	GAANNNNTTC	2 cut(s)	1493, 1878
Asp718I	GGTACC	1 cut(s)	1785
AspLEI	GCGC	3 cut(s)	908, 935, 1055
AspS9I	GGNCC	2 cut(s)	228, 650
AsuC2I	CCSGG	1 cut(s)	564
AsuHPI	GGTGA	4 cut(s)	82, 125, 745, 1730
AvaI	CYCGRG	1 cut(s)	578
AvaII	GGWCC	2 cut(s)	228, 650
BamHI	GGATCC	1 cut(s)	553
BanI	GGYRCC	2 cut(s)	905, 1785
BanII	GRGCYC	2 cut(s)	153, 1227
BauI	CACGAG	1 cut(s)	1829
Bbv12I	GWGCWC	3 cut(s)	153, 1227, 1628
BbvI	GCAGC	4 cut(s)	89, 465, 1301, 1342
BccI	CCATC	4 cut(s)	95, 293, 790, 1447
BceAI	ACGGC	1 cut(s)	424
BciT130I	CCWGG	5 cut(s)	168, 401, 483, 844, 1262
BciVI	GTATCC	2 cut(s)	229, 793
BclI	TGATCA	2 cut(s)	115, 457
BcnI	CCSGG	1 cut(s)	564
BcoDI	GTCTC	3 cut(s)	73, 133, 870
BcuI	ACTAGT	1 cut(s)	214
BfaI	CTAG	6 cut(s)	215, 420, 489, 630, 1050, 1089
BfoI	RGCGCY	2 cut(s)	909, 936
BfuI	GTATCC	2 cut(s)	229, 793
BglI	GCCNNNNNGGC	2 cut(s)	445, 844
BisI	GCNGC	5 cut(s)	78, 369, 479, 1290, 1356
BlpI	GCTNAGC	2 cut(s)	1351, 1363
BlsI	GCNGC	5 cut(s)	79, 370, 480, 1291, 1357
Bme1390I	CCNGG	6 cut(s)	168, 401, 483, 564, 844, 1262
Bme18I	GGWCC	2 cut(s)	228, 650
BmeT110I	CYCGRG	1 cut(s)	578
BmgT120I	GGNCC	2 cut(s)	228, 650
BmiI	GGNNCC	5 cut(s)	555, 687, 837, 907, 1787
BmrFI	CCNGG	6 cut(s)	168, 401, 483, 564, 844, 1262
BmsI	GCATC	9 cut(s)	379, 592, 649, 872, 1464, 1471, 1765, 1857, 1996
Bpu1102I	GCTNAGC	2 cut(s)	1351, 1363
BpuEI	CTTGAG	2 cut(s)	427, 1789
BpuMI	CCSGG	1 cut(s)	564
BsaBI	GATNNNNATC	1 cut(s)	1755
BsaHI	GRCGYC	1 cut(s)	906
BsaI	GGTCTC	1 cut(s)	870
BsaJI	CCNNGG	3 cut(s)	653, 1261, 1594
BsaWI	WCCGGW	3 cut(s)	110, 688, 1071
Bsc4I	CCNNNNNNNGG	5 cut(s)	237, 339, 563, 849, 1909
Bse118I	RCCGGY	3 cut(s)	134, 1071, 1358
Bse1I	ACTGG	1 cut(s)	1426
Bse3DI	GCAATG	3 cut(s)	854, 1097, 1544
Bse8I	GATNNNNATC	1 cut(s)	1755
BseAI	TCCGGA	1 cut(s)	688
BseBI	CCWGG	5 cut(s)	168, 401, 483, 844, 1262
BseDI	CCNNGG	3 cut(s)	653, 1261, 1594
BseGI	GGATG	6 cut(s)	394, 460, 664, 1208, 1701, 1756
BseJI	GATNNNNATC	1 cut(s)	1755
BseLI	CCNNNNNNNGG	5 cut(s)	237, 339, 563, 849, 1909
BseMI	GCAATG	3 cut(s)	854, 1097, 1544
BseMII	CTCAG	3 cut(s)	1287, 1294, 1365
BseNI	ACTGG	1 cut(s)	1426
BseRI	GAGGAG	3 cut(s)	349, 352, 1530
BseXI	GCAGC	4 cut(s)	89, 465, 1301, 1342
BseYI	CCCAGC	1 cut(s)	681
BsgI	GTGCAG	2 cut(s)	360, 543
Bsh1236I	CGCG	2 cut(s)	1055, 1668
BshFI	GGCC	2 cut(s)	1380, 1923
BshNI	GGYRCC	2 cut(s)	905, 1785
BshTI	ACCGGT	1 cut(s)	1071
BsiHKAI	GWGCWC	3 cut(s)	153, 1227, 1628
BsiHKCI	CYCGRG	1 cut(s)	578
BsiSI	CCGG	9 cut(s)	111, 135, 393, 464, 563, 689, 1072, 1359, 1871
BslFI	GGGAC	5 cut(s)	1416, 1432, 1555, 1648, 1816
BslI	CCNNNNNNNGG	5 cut(s)	237, 339, 563, 849, 1909
BsmAI	GTCTC	3 cut(s)	73, 133, 870
BsmFI	GGGAC	5 cut(s)	1416, 1432, 1555, 1648, 1816
BsnI	GGCC	2 cut(s)	1380, 1923
Bso31I	GGTCTC	1 cut(s)	870
BsoBI	CYCGRG	1 cut(s)	578
Bsp1286I	GDGCHC	3 cut(s)	153, 1227, 1628
Bsp13I	TCCGGA	1 cut(s)	688
Bsp1407I	TGTACA	1 cut(s)	1990
Bsp143I	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
Bsp1720I	GCTNAGC	2 cut(s)	1351, 1363
Bsp19I	CCATGG	1 cut(s)	653
BspACI	CCGC	7 cut(s)	93, 132, 366, 369, 1139, 1660, 1668
BspANI	GGCC	2 cut(s)	1380, 1923
BspCNI	CTCAG	3 cut(s)	1288, 1293, 1364
BspEI	TCCGGA	1 cut(s)	688
BspFNI	CGCG	2 cut(s)	1055, 1668
BspLI	GGNNCC	5 cut(s)	555, 687, 837, 907, 1787
BspPI	GGATC	3 cut(s)	548, 561, 764
BspT107I	GGYRCC	2 cut(s)	905, 1785
BspTNI	GGTCTC	1 cut(s)	870
BsrDI	GCAATG	3 cut(s)	854, 1097, 1544
BsrFI	RCCGGY	3 cut(s)	134, 1071, 1358
BsrGI	TGTACA	1 cut(s)	1990
BsrI	ACTGG	1 cut(s)	1426
BssAI	RCCGGY	3 cut(s)	134, 1071, 1358
BssECI	CCNNGG	3 cut(s)	653, 1261, 1594
BssMI	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
BssNI	GRCGYC	1 cut(s)	906
BssSI	CACGAG	1 cut(s)	1829
BssT1I	CCWWGG	2 cut(s)	653, 1594
Bst2BI	CACGAG	1 cut(s)	1829
Bst2UI	CCWGG	5 cut(s)	168, 401, 483, 844, 1262
Bst4CI	ACNGT	3 cut(s)	618, 959, 1976
Bst6I	CTCTTC	3 cut(s)	536, 971, 1936
BstACI	GRCGYC	1 cut(s)	906
BstAPI	GCANNNNNTGC	1 cut(s)	1845
BstAUI	TGTACA	1 cut(s)	1990
BstC8I	GCNNGC	2 cut(s)	1348, 1360
BstDEI	CTNAG	5 cut(s)	1160, 1280, 1296, 1351, 1363
BstDSI	CCRYGG	1 cut(s)	653
BstF5I	GGATG	6 cut(s)	394, 460, 664, 1208, 1701, 1756
BstFNI	CGCG	2 cut(s)	1055, 1668
BstH2I	RGCGCY	2 cut(s)	909, 936
BstHHI	GCGC	3 cut(s)	908, 935, 1055
BstKTI	GATC	7 cut(s)	118, 460, 556, 772, 1030, 1753, 1972
BstMAI	GTCTC	3 cut(s)	73, 133, 870
BstMBI	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
BstMWI	GCNNNNNNNGC	8 cut(s)	374, 445, 844, 1052, 1352, 1364, 1674, 1845
BstNI	CCWGG	5 cut(s)	168, 401, 483, 844, 1262
BstNSI	RCATGY	3 cut(s)	1096, 1649, 1717
BstSCI	CCNGG	6 cut(s)	166, 399, 481, 562, 842, 1260
BstUI	CGCG	2 cut(s)	1055, 1668
BstV1I	GCAGC	4 cut(s)	89, 465, 1301, 1342
BstX2I	RGATCY	1 cut(s)	553
BstYI	RGATCY	1 cut(s)	553
BsuI	GTATCC	2 cut(s)	229, 793
BsuRI	GGCC	2 cut(s)	1380, 1923
BtgI	CCRYGG	1 cut(s)	653
BtgZI	GCGATG	1 cut(s)	615
BtsCI	GGATG	6 cut(s)	394, 460, 664, 1208, 1701, 1756
BtsI	GCAGTG	3 cut(s)	372, 1176, 1575
BtsIMutI	CAGTG	4 cut(s)	372, 1176, 1575, 1981
Cac8I	GCNNGC	2 cut(s)	1348, 1360
CaiI	CAGNNNCTG	1 cut(s)	1295
CfoI	GCGC	3 cut(s)	908, 935, 1055
Cfr10I	RCCGGY	3 cut(s)	134, 1071, 1358
Cfr13I	GGNCC	2 cut(s)	228, 650
CseI	GACGC	1 cut(s)	1898
CsiI	ACCWGGT	2 cut(s)	166, 399
Csp6I	GTAC	5 cut(s)	202, 429, 1465, 1786, 1991
CspAI	ACCGGT	1 cut(s)	1071
CviAII	CATG	9 cut(s)	65, 474, 537, 654, 828, 1093, 1646, 1714, 1951
CviQI	GTAC	5 cut(s)	202, 429, 1465, 1786, 1991
DdeI	CTNAG	5 cut(s)	1160, 1280, 1296, 1351, 1363
DinI	GGCGCC	1 cut(s)	907
DpnI	GATC	7 cut(s)	117, 459, 555, 771, 1029, 1752, 1971
DpnII	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
Eam1104I	CTCTTC	3 cut(s)	536, 971, 1936
EarI	CTCTTC	3 cut(s)	536, 971, 1936
EciI	GGCGGA	3 cut(s)	121, 355, 1649
Ecl136II	GAGCTC	2 cut(s)	151, 1225
Eco130I	CCWWGG	2 cut(s)	653, 1594
Eco147I	AGGCCT	1 cut(s)	1380
Eco24I	GRGCYC	2 cut(s)	153, 1227
Eco31I	GGTCTC	1 cut(s)	870
Eco47I	GGWCC	2 cut(s)	228, 650
Eco53kI	GAGCTC	2 cut(s)	151, 1225
Eco57I	CTGAAG	2 cut(s)	703, 1701
Eco88I	CYCGRG	1 cut(s)	578
EcoICRI	GAGCTC	2 cut(s)	151, 1225
EcoRI	GAATTC	1 cut(s)	1306
EcoRII	CCWGG	5 cut(s)	166, 399, 481, 842, 1260
EcoT14I	CCWWGG	2 cut(s)	653, 1594
EcoT38I	GRGCYC	2 cut(s)	153, 1227
EgeI	GGCGCC	1 cut(s)	907
EheI	GGCGCC	1 cut(s)	907
ErhI	CCWWGG	2 cut(s)	653, 1594
FaeI	CATG	9 cut(s)	68, 477, 540, 657, 831, 1096, 1649, 1717, 1954
FaqI	GGGAC	5 cut(s)	1416, 1432, 1555, 1648, 1816
FatI	CATG	9 cut(s)	64, 473, 536, 653, 827, 1092, 1645, 1713, 1950
FauI	CCCGC	1 cut(s)	1661
FauNDI	CATATG	1 cut(s)	197
FbaI	TGATCA	2 cut(s)	115, 457
FblI	GTMKAC	1 cut(s)	1257
Fnu4HI	GCNGC	5 cut(s)	78, 369, 479, 1290, 1356
FokI	GGATG	6 cut(s)	401, 447, 671, 1215, 1708, 1743
FriOI	GRGCYC	2 cut(s)	153, 1227
Fsp4HI	GCNGC	5 cut(s)	78, 369, 479, 1290, 1356
FspBI	CTAG	6 cut(s)	215, 420, 489, 630, 1050, 1089
GlaI	GCGC	3 cut(s)	907, 934, 1054
GluI	GCNGC	5 cut(s)	78, 369, 479, 1290, 1356
GsaI	CCCAGC	1 cut(s)	685
HaeII	RGCGCY	2 cut(s)	909, 936
HaeIII	GGCC	2 cut(s)	1380, 1923
HapII	CCGG	9 cut(s)	111, 135, 393, 464, 563, 689, 1072, 1359, 1871
HgaI	GACGC	1 cut(s)	1898
HhaI	GCGC	3 cut(s)	908, 935, 1055
Hin1I	GRCGYC	1 cut(s)	906
Hin1II	CATG	9 cut(s)	68, 477, 540, 657, 831, 1096, 1649, 1717, 1954
Hin6I	GCGC	3 cut(s)	906, 933, 1053
HinP1I	GCGC	3 cut(s)	906, 933, 1053
HincII	GTYRAC	3 cut(s)	345, 1258, 1651
HindII	GTYRAC	3 cut(s)	345, 1258, 1651
HindIII	AAGCTT	4 cut(s)	451, 671, 1577, 1931
HinfI	GANTC	7 cut(s)	83, 323, 789, 814, 1148, 1553, 1733
HpaII	CCGG	9 cut(s)	111, 135, 393, 464, 563, 689, 1072, 1359, 1871
HphI	GGTGA	4 cut(s)	82, 125, 745, 1730
Hpy166II	GTNNAC	6 cut(s)	204, 345, 650, 1258, 1651, 1991
Hpy188III	TCNNGA	9 cut(s)	406, 689, 786, 793, 926, 1079, 1228, 1768, 1829
Hpy8I	GTNNAC	6 cut(s)	204, 345, 650, 1258, 1651, 1991
HpyAV	CCTTC	5 cut(s)	849, 919, 1211, 1673, 1676
HpyCH4III	ACNGT	3 cut(s)	618, 959, 1976
HpyCH4IV	ACGT	1 cut(s)	431
HpyF10VI	GCNNNNNNNGC	8 cut(s)	374, 445, 844, 1052, 1352, 1364, 1674, 1845
HpyF3I	CTNAG	5 cut(s)	1160, 1280, 1296, 1351, 1363
HpySE526I	ACGT	1 cut(s)	431
Hsp92I	GRCGYC	1 cut(s)	906
Hsp92II	CATG	9 cut(s)	68, 477, 540, 657, 831, 1096, 1649, 1717, 1954
HspAI	GCGC	3 cut(s)	906, 933, 1053
KasI	GGCGCC	1 cut(s)	905
Kpn2I	TCCGGA	1 cut(s)	688
KpnI	GGTACC	1 cut(s)	1789
KroI	GCCGGC	1 cut(s)	1358
KroNI	GCCGGC	1 cut(s)	1360
Ksp22I	TGATCA	2 cut(s)	115, 457
Kzo9I	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
LmnI	GCTCC	3 cut(s)	485, 597, 835
Lsp1109I	GCAGC	4 cut(s)	89, 465, 1301, 1342
LweI	GCATC	9 cut(s)	379, 592, 649, 872, 1464, 1471, 1765, 1857, 1996
MabI	ACCWGGT	2 cut(s)	166, 399
MaeI	CTAG	6 cut(s)	215, 420, 489, 630, 1050, 1089
MaeII	ACGT	1 cut(s)	431
MaeIII	GTNAC	5 cut(s)	508, 727, 746, 1316, 1859
MalI	GATC	7 cut(s)	117, 459, 555, 771, 1029, 1752, 1971
MboI	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
MboII	GAAGA	9 cut(s)	37, 151, 168, 270, 553, 988, 1585, 1817, 1953
MfeI	CAATTG	1 cut(s)	1629
MflI	RGATCY	1 cut(s)	553
MhlI	GDGCHC	3 cut(s)	153, 1227, 1628
Mly113I	GGCGCC	1 cut(s)	906
MlyI	GAGTC	3 cut(s)	77, 332, 1727
MmeI	TCCRAC	4 cut(s)	813, 1666, 1754, 1918
MroI	TCCGGA	1 cut(s)	688
MroNI	GCCGGC	1 cut(s)	1358
MroXI	GAANNNNTTC	2 cut(s)	1493, 1878
MseI	TTAA	5 cut(s)	287, 318, 353, 501, 1302
MslI	CAYNNNNRTG	2 cut(s)	1568, 1761
MspA1I	CMGCKG	3 cut(s)	681, 823, 1355
MspI	CCGG	9 cut(s)	111, 135, 393, 464, 563, 689, 1072, 1359, 1871
MspR9I	CCNGG	6 cut(s)	168, 401, 483, 564, 844, 1262
MunI	CAATTG	1 cut(s)	1629
MvaI	CCWGG	5 cut(s)	168, 401, 483, 844, 1262
MvnI	CGCG	2 cut(s)	1055, 1668
MwoI	GCNNNNNNNGC	8 cut(s)	374, 445, 844, 1052, 1352, 1364, 1674, 1845
NaeI	GCCGGC	1 cut(s)	1360
NarI	GGCGCC	1 cut(s)	906
NciI	CCSGG	1 cut(s)	564
NcoI	CCATGG	1 cut(s)	653
NdeI	CATATG	1 cut(s)	197
NdeII	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
NgoMIV	GCCGGC	1 cut(s)	1358
NlaIII	CATG	9 cut(s)	68, 477, 540, 657, 831, 1096, 1649, 1717, 1954
NlaIV	GGNNCC	5 cut(s)	555, 687, 837, 907, 1787
NmuCI	GTSAC	1 cut(s)	1859
NspI	RCATGY	3 cut(s)	1096, 1649, 1717
PaeR7I	CTCGAG	1 cut(s)	578
PceI	AGGCCT	1 cut(s)	1380
PciI	ACATGT	1 cut(s)	1645
PcsI	WCGNNNNNNNCGW	3 cut(s)	437, 1454, 1965
PdiI	GCCGGC	1 cut(s)	1360
PdmI	GAANNNNTTC	2 cut(s)	1493, 1878
PfeI	GAWTC	4 cut(s)	789, 814, 1148, 1553
PflMI	CCANNNNNTGG	2 cut(s)	237, 849
PinAI	ACCGGT	1 cut(s)	1071
PkrI	GCNGC	5 cut(s)	79, 370, 480, 1291, 1357
PleI	GAGTC	3 cut(s)	77, 331, 1727
PluTI	GGCGCC	1 cut(s)	909
PpsI	GAGTC	3 cut(s)	77, 331, 1727
PscI	ACATGT	1 cut(s)	1645
PsiI	TTATAA	1 cut(s)	1191
Psp124BI	GAGCTC	2 cut(s)	153, 1227
Psp6I	CCWGG	5 cut(s)	166, 399, 481, 842, 1260
PspFI	CCCAGC	1 cut(s)	681
PspGI	CCWGG	5 cut(s)	166, 399, 481, 842, 1260
PspN4I	GGNNCC	5 cut(s)	555, 687, 837, 907, 1787
PspPI	GGNCC	2 cut(s)	228, 650
PspXI	VCTCGAGB	1 cut(s)	578
PstNI	CAGNNNCTG	1 cut(s)	1295
PsuI	RGATCY	1 cut(s)	553
PvuII	CAGCTG	3 cut(s)	681, 823, 1355
RsaI	GTAC	5 cut(s)	203, 430, 1466, 1787, 1992
RsaNI	GTAC	5 cut(s)	202, 429, 1465, 1786, 1991
RseI	CAYNNNNRTG	2 cut(s)	1568, 1761
SacI	GAGCTC	2 cut(s)	153, 1227
SalI	GTCGAC	1 cut(s)	1256
SaqAI	TTAA	5 cut(s)	287, 318, 353, 501, 1302
SatI	GCNGC	5 cut(s)	78, 369, 479, 1290, 1356
Sau3AI	GATC	7 cut(s)	115, 457, 553, 769, 1027, 1750, 1969
Sau96I	GGNCC	2 cut(s)	228, 650
SchI	GAGTC	3 cut(s)	77, 332, 1727
ScrFI	CCNGG	6 cut(s)	168, 401, 483, 564, 844, 1262
SduI	GDGCHC	3 cut(s)	153, 1227, 1628
SexAI	ACCWGGT	2 cut(s)	166, 399
SfaNI	GCATC	9 cut(s)	379, 592, 649, 872, 1464, 1471, 1765, 1857, 1996
SfoI	GGCGCC	1 cut(s)	907
Sfr274I	CTCGAG	1 cut(s)	578
SgrAI	CRCCGGYG	1 cut(s)	134
SinI	GGWCC	2 cut(s)	228, 650
SlaI	CTCGAG	1 cut(s)	578
SmiMI	CAYNNNNRTG	2 cut(s)	1568, 1761
SmlI	CTYRAG	3 cut(s)	406, 578, 1768
SmoI	CTYRAG	3 cut(s)	406, 578, 1768
SpeI	ACTAGT	1 cut(s)	214
SseBI	AGGCCT	1 cut(s)	1380
SsiI	CCGC	7 cut(s)	93, 132, 366, 369, 1139, 1660, 1668
SspDI	GGCGCC	1 cut(s)	905
SspI	AATATT	1 cut(s)	1135
SspMI	CTAG	6 cut(s)	215, 420, 489, 630, 1050, 1089
SstI	GAGCTC	2 cut(s)	153, 1227
StuI	AGGCCT	1 cut(s)	1380
StyD4I	CCNGG	6 cut(s)	166, 399, 481, 562, 842, 1260
StyI	CCWWGG	2 cut(s)	653, 1594
TaaI	ACNGT	3 cut(s)	618, 959, 1976
TaiI	ACGT	1 cut(s)	434
TaqI	TCGA	5 cut(s)	147, 579, 787, 1208, 1257
TatI	WGTACW	1 cut(s)	1990
TauI	GCSGC	1 cut(s)	371
TfiI	GAWTC	4 cut(s)	789, 814, 1148, 1553
Tru1I	TTAA	5 cut(s)	287, 318, 353, 501, 1302
Tru9I	TTAA	5 cut(s)	287, 318, 353, 501, 1302
TscAI	CASTG	4 cut(s)	379, 1183, 1575, 1981
TseFI	GTSAC	1 cut(s)	1859
TseI	GCWGC	4 cut(s)	77, 478, 1289, 1355
Tsp45I	GTSAC	1 cut(s)	1859
TspDTI	ATGAA	4 cut(s)	525, 1125, 1545, 1939
TspGWI	ACGGA	1 cut(s)	2014
TspRI	CASTG	4 cut(s)	379, 1183, 1575, 1981
Van91I	CCANNNNNTGG	2 cut(s)	237, 849
VpaK11BI	GGWCC	2 cut(s)	228, 650
XapI	RAATTY	4 cut(s)	349, 525, 1231, 1306
XceI	RCATGY	3 cut(s)	1096, 1649, 1717
XcmI	CCANNNNNNNNNTGG	3 cut(s)	22, 238, 1796
XhoI	CTCGAG	1 cut(s)	578
XmiI	GTMKAC	1 cut(s)	1257
XmnI	GAANNNNTTC	2 cut(s)	1493, 1878
XspI	CTAG	6 cut(s)	215, 420, 489, 630, 1050, 1089

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.