pycom09g04160

Mitochondrial pyruvate carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0005603
Physical Location & Seq
Forward (+)
3062983 .. 3065103
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGCCTTCGGAAGAGGCTGAAATACTCGATCGATCTTGTAACTTTAGTGGTAGAAAGCGTTTACCCACACTCAAAGTCAAGCTTAAATCTCACCTCCTCTATACTTCACTTTCTTTGAATTCAACCCCAAATTATTGGAAGGACCACTATGATAATGATTTTATATATAAGCTTTTATCTTCTTCTTCCTCAGTTATGCTGTATATTCAATCAGATCTCCCGAAGGGATCCGAGAATCATTTCATGGCAAATTCCAAGCTGCAAGCATTTTGGAACCACCCAGCTGGCCCCAAAACGATTCATTTCTGGGCCCCAACATTTAAATGGGGTCTAACCATTGCAAATATTCTTGACTCAGCAACACCACCAGAGAAACTATCCTATCCTCAACAATCAGTCCTTGCATGCAGTGGACTAATATGGGCAAGATACAGCACAGTAATTATACCAAAGAACTGGAATCTTGTGAGTGTCAATTTCGCTATGTCTGTAACCGCCATGTATCAACTTTCCCGTAAAATTCAGCATGACTTGTCTATCAAAAACCAAGAAGTTGCTGCAGAAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPC

Protein Analysis

191

Amino Acids

21.66

Weight (kDa)

8.79

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MPC PF03650 87 - 187 3.4e-37 Mitochondrial pyruvate carriers
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 495
AclWI GGATC 2 cut(s) 222, 235
AcsI RAATTY 3 cut(s) 118, 250, 519
AgsI TTSAA 3 cut(s) 118, 123, 209
AluBI AGCT 4 cut(s) 82, 172, 259, 284
AluI AGCT 4 cut(s) 82, 172, 259, 284
AlwI GGATC 2 cut(s) 222, 235
AoxI GGCC 2 cut(s) 286, 309
ApaI GGGCCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 259, 557
ApoI RAATTY 3 cut(s) 118, 250, 519
Asp700I GAANNNNTTC 1 cut(s) 239
AspS9I GGNCC 4 cut(s) 142, 287, 309, 310
AsuHPI GGTGA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 313
BamHI GGATCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 246, 544
BfmI CTRYAG 1 cut(s) 558
BglII AGATCT 1 cut(s) 214
BisI GCNGC 2 cut(s) 260, 558
BlsI GCNGC 2 cut(s) 261, 559
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 4 cut(s) 142, 287, 309, 310
BmiI GGNNCC 5 cut(s) 229, 275, 289, 311, 312
Bsa29I ATCGAT 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 461
Bse3DI GCAATG 1 cut(s) 336
BseCI ATCGAT 1 cut(s) 31
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 204, 369
BseNI ACTGG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 2 cut(s) 246, 544
BseYI CCCAGC 1 cut(s) 280
Bsh1285I CGRYCG 1 cut(s) 31
BshFI GGCC 2 cut(s) 288, 311
BshVI ATCGAT 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 31
BsnI GGCC 2 cut(s) 288, 311
Bsp120I GGGCCC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 4 cut(s) 28, 32, 214, 227
BspACI CCGC 1 cut(s) 495
BspANI GGCC 2 cut(s) 288, 311
BspCNI CTCAG 2 cut(s) 203, 368
BspDI ATCGAT 1 cut(s) 31
BspLI GGNNCC 5 cut(s) 229, 275, 289, 311, 312
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 2 cut(s) 222, 235
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 1 cut(s) 461
BssMI GATC 4 cut(s) 28, 32, 214, 227
Bst4CI ACNGT 1 cut(s) 439
Bst6I CTCTTC 1 cut(s) 6
BstC8I GCNNGC 3 cut(s) 264, 286, 406
BstDEI CTNAG 2 cut(s) 190, 355
BstKTI GATC 4 cut(s) 31, 35, 217, 230
BstMBI GATC 4 cut(s) 28, 32, 214, 227
BstMCI CGRYCG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 408
BstSFI CTRYAG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 246, 544
BstX2I RGATCY 2 cut(s) 214, 227
BstXI CCANNNNNNTGG 3 cut(s) 135, 284, 456
BstYI RGATCY 2 cut(s) 214, 227
Bsu15I ATCGAT 1 cut(s) 31
BsuRI GGCC 2 cut(s) 288, 311
BsuTUI ATCGAT 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 264, 286, 406
Cfr13I GGNCC 4 cut(s) 142, 287, 309, 310
ClaI ATCGAT 1 cut(s) 31
CviAII CATG 4 cut(s) 244, 405, 499, 527
CviJI RGCY 7 cut(s) 17, 82, 172, 259, 284, 288, 311
CviKI_1 RGCY 7 cut(s) 17, 82, 172, 259, 284, 288, 311
DdeI CTNAG 2 cut(s) 190, 355
DpnI GATC 4 cut(s) 30, 34, 216, 229
DpnII GATC 4 cut(s) 28, 32, 214, 227
DraI TTTAAA 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 313
FaeI CATG 4 cut(s) 247, 408, 502, 530
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FatI CATG 4 cut(s) 243, 404, 498, 526
Fnu4HI GCNGC 2 cut(s) 260, 558
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 260, 558
GluI GCNGC 2 cut(s) 260, 558
GsaI CCCAGC 1 cut(s) 284
HaeIII GGCC 2 cut(s) 288, 311
Hin1II CATG 4 cut(s) 247, 408, 502, 530
HindIII AAGCTT 2 cut(s) 80, 170
HinfI GANTC 4 cut(s) 235, 298, 353, 460
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 2 cut(s) 62, 413
Hpy188I TCNGA 3 cut(s) 10, 214, 232
Hpy188III TCNNGA 2 cut(s) 220, 350
Hpy8I GTNNAC 2 cut(s) 62, 413
HpyAV CCTTC 3 cut(s) 15, 133, 217
HpyCH4III ACNGT 1 cut(s) 439
HpyCH4V TGCA 5 cut(s) 262, 341, 404, 408, 560
HpyF3I CTNAG 2 cut(s) 190, 355
Hsp92II CATG 4 cut(s) 247, 408, 502, 530
Kzo9I GATC 4 cut(s) 28, 32, 214, 227
LpnPI CCDG 5 cut(s) 270, 292, 294, 381, 442
Lsp1109I GCAGC 2 cut(s) 246, 544
MaeIII GTNAC 2 cut(s) 38, 490
MalI GATC 4 cut(s) 30, 34, 216, 229
MboI GATC 4 cut(s) 28, 32, 214, 227
MboII GAAGA 4 cut(s) 23, 171, 174, 177
MflI RGATCY 2 cut(s) 214, 227
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 6 cut(s) 118, 130, 250, 441, 475, 519
MlyI GAGTC 1 cut(s) 347
MnlI CCTC 5 cut(s) 7, 104, 107, 199, 396
MroXI GAANNNNTTC 1 cut(s) 239
MseI TTAA 2 cut(s) 84, 321
MslI CAYNNNNRTG 1 cut(s) 322
MspA1I CMGCKG 1 cut(s) 284
NdeII GATC 4 cut(s) 28, 32, 214, 227
NlaIII CATG 4 cut(s) 247, 408, 502, 530
NlaIV GGNNCC 5 cut(s) 229, 275, 289, 311, 312
NspI RCATGY 1 cut(s) 408
PaeI GCATGC 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 3 cut(s) 235, 298, 460
PkrI GCNGC 2 cut(s) 261, 559
Ple19I CGATCG 1 cut(s) 31
PleI GAGTC 1 cut(s) 347
PpsI GAGTC 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 280
PspN4I GGNNCC 5 cut(s) 229, 275, 289, 311, 312
PspOMI GGGCCC 1 cut(s) 309
PspPI GGNCC 4 cut(s) 142, 287, 309, 310
PstI CTGCAG 1 cut(s) 562
PsuI RGATCY 2 cut(s) 214, 227
PvuI CGATCG 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 322
SaqAI TTAA 2 cut(s) 84, 321
SatI GCNGC 2 cut(s) 260, 558
Sau3AI GATC 4 cut(s) 28, 32, 214, 227
Sau96I GGNCC 4 cut(s) 142, 287, 309, 310
SchI GAGTC 1 cut(s) 347
SduI GDGCHC 1 cut(s) 313
SetI ASST 5 cut(s) 84, 96, 174, 261, 286
SfcI CTRYAG 1 cut(s) 558
SinI GGWCC 1 cut(s) 142
SmiI ATTTAAAT 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 322
SphI GCATGC 1 cut(s) 408
Sse9I AATT 6 cut(s) 118, 130, 250, 441, 475, 519
SsiI CCGC 1 cut(s) 495
SspI AATATT 1 cut(s) 346
SwaI ATTTAAAT 1 cut(s) 322
TaaI ACNGT 1 cut(s) 439
TaqI TCGA 2 cut(s) 27, 31
TasI AATT 6 cut(s) 118, 130, 250, 441, 475, 519
TfiI GAWTC 3 cut(s) 235, 298, 460
Tru1I TTAA 2 cut(s) 84, 321
Tru9I TTAA 2 cut(s) 84, 321
TscAI CASTG 1 cut(s) 415
TseI GCWGC 2 cut(s) 259, 557
TspDTI ATGAA 2 cut(s) 232, 290
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 142
XapI RAATTY 3 cut(s) 118, 250, 519
XceI RCATGY 1 cut(s) 408
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.