Rorug02G0462500

Mitochondrial pyruvate carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005603
Physical Location & Seq
Reverse (-)
59387842 .. 59388306
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0462500.1

Sequence Viewer

Length: 465 bp
ATGGATCCTTTTACTCTCTCCAACAATCATCAAGCATCAAGCAAACATATTAGCGCTTTTGATTTTGGGAGTTCCAGCTTTGCCACCATGTCTAGAGCAGCTCAAGTGATATTTTGCCTCGTGCTAATGGTAACATGCGCCGCAACTGGGCTAGCGCATCAGGCACCCCCAGCACCACCTCCCCTCTTTAACATTCCAGGCCTATTGCCGCCAGGGTTCCCCGGCCTACTGCCGCCAACAAACCCAAATGACATAGCAAAGTGTTGGTCTTCACTTCAGAACATTCAAGGGTGTGTATCGGAAATCTTTACCTCAATCTTTACTCACAAAATAGAAGTAGGCCCTGCCTGTTGTAAAGCTTTCCTTGCAATTGAAGAGAGTTGCTGGCCGAAAATGTTCCCCTTAACTCCTTTCTTTGCTCCATTGCTCAAGAACACTTGTGCTCAGAAGGCAGCTCTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MPC

Protein Analysis

154

Amino Acids

16.46

Weight (kDa)

7.57

Isoelectric Point (pI)

68.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 87 - 147 1.4e-17 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AciI CCGC 3 cut(s) 141, 209, 233
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 260
AfeI AGCGCT 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 2 cut(s) 287, 374
AjnI CCWGG 2 cut(s) 196, 211
AluBI AGCT 4 cut(s) 78, 101, 359, 455
AluI AGCT 4 cut(s) 78, 101, 359, 455
Alw21I GWGCWC 1 cut(s) 445
AlwI GGATC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 55
AoxI GGCC 4 cut(s) 199, 223, 340, 386
ApeKI GCWGC 2 cut(s) 98, 452
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 3 cut(s) 56, 140, 157
AspS9I GGNCC 1 cut(s) 341
AsuC2I CCSGG 1 cut(s) 222
AsuNHI GCTAGC 1 cut(s) 151
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 163
BauI CACGAG 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 1 cut(s) 110
BciT130I CCWGG 2 cut(s) 198, 213
BcnI CCSGG 1 cut(s) 222
BfaI CTAG 2 cut(s) 93, 152
BfoI RGCGCY 1 cut(s) 57
BisI GCNGC 5 cut(s) 99, 141, 209, 233, 453
BlsI GCNGC 5 cut(s) 100, 142, 210, 234, 454
Bme1390I CCNGG 3 cut(s) 198, 213, 222
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 3 cut(s) 6, 165, 218
BmrFI CCNGG 3 cut(s) 198, 213, 222
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 2 cut(s) 44, 166
BmtI GCTAGC 1 cut(s) 155
BmuI ACTGGG 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 87, 413
BpuMI CCSGG 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 212, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 422
BseBI CCWGG 2 cut(s) 198, 213
BseDI CCNNGG 2 cut(s) 212, 220
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 422
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 110
BseYI CCCAGC 1 cut(s) 169
BshFI GGCC 4 cut(s) 201, 225, 342, 388
BshNI GGYRCC 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 445
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 147
BsnI GGCC 4 cut(s) 201, 225, 342, 388
Bsp1286I GDGCHC 1 cut(s) 445
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 3 cut(s) 141, 209, 233
BspANI GGCC 4 cut(s) 201, 225, 342, 388
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 3 cut(s) 6, 165, 218
BspOI GCTAGC 1 cut(s) 155
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 422
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 212, 220
BssMI GATC 1 cut(s) 4
BssSI CACGAG 1 cut(s) 119
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 2 cut(s) 198, 213
Bst6I CTCTTC 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 153, 386
BstDEI CTNAG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 3 cut(s) 56, 140, 157
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 4 cut(s) 161, 170, 365, 449
BstNI CCWGG 2 cut(s) 198, 213
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 3 cut(s) 196, 211, 220
BstV1I GCAGC 1 cut(s) 110
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 4 cut(s) 201, 225, 342, 388
Cac8I GCNNGC 2 cut(s) 153, 386
CfoI GCGC 3 cut(s) 56, 140, 157
Cfr13I GGNCC 1 cut(s) 341
CviAII CATG 2 cut(s) 88, 135
CviJI RGCY 9 cut(s) 78, 101, 151, 201, 225, 342, 359, 388, 455
CviKI_1 RGCY 9 cut(s) 78, 101, 151, 201, 225, 342, 359, 388, 455
DdeI CTNAG 1 cut(s) 444
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 201
Eco47III AGCGCT 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 196, 211
FaeI CATG 2 cut(s) 91, 138
FaiI YATR 4 cut(s) 48, 89, 136, 254
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 2 cut(s) 87, 134
Fnu4HI GCNGC 5 cut(s) 99, 141, 209, 233, 453
Fsp4HI GCNGC 5 cut(s) 99, 141, 209, 233, 453
FspBI CTAG 2 cut(s) 93, 152
GlaI GCGC 3 cut(s) 55, 139, 156
GluI GCNGC 5 cut(s) 99, 141, 209, 233, 453
GsaI CCCAGC 1 cut(s) 173
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 4 cut(s) 201, 225, 342, 388
HapII CCGG 1 cut(s) 222
HhaI GCGC 3 cut(s) 56, 140, 157
Hin1II CATG 2 cut(s) 91, 138
Hin6I GCGC 3 cut(s) 54, 138, 155
HinP1I GCGC 3 cut(s) 54, 138, 155
HindIII AAGCTT 1 cut(s) 357
HpaII CCGG 1 cut(s) 222
Hpy188I TCNGA 3 cut(s) 279, 301, 447
Hpy188III TCNNGA 2 cut(s) 93, 430
HpyAV CCTTC 1 cut(s) 442
HpyCH4V TGCA 1 cut(s) 368
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 170, 365, 449
HpyF3I CTNAG 1 cut(s) 444
Hsp92II CATG 2 cut(s) 91, 138
HspAI GCGC 3 cut(s) 54, 138, 155
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 2 cut(s) 44, 166
MaeI CTAG 2 cut(s) 93, 152
MaeIII GTNAC 1 cut(s) 130
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 261, 386
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 4 cut(s) 128, 189, 194, 322
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 3 cut(s) 189, 404, 463
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 3 cut(s) 198, 213, 222
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 198, 213
MwoI GCNNNNNNNGC 4 cut(s) 161, 170, 365, 449
NciI CCSGG 1 cut(s) 222
NdeII GATC 1 cut(s) 4
NheI GCTAGC 1 cut(s) 151
NlaIII CATG 2 cut(s) 91, 138
NlaIV GGNNCC 3 cut(s) 6, 165, 218
NspI RCATGY 1 cut(s) 138
PceI AGGCCT 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 395
PkrI GCNGC 5 cut(s) 100, 142, 210, 234, 454
Psp6I CCWGG 2 cut(s) 196, 211
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 2 cut(s) 196, 211
PspN4I GGNNCC 3 cut(s) 6, 165, 218
PspPI GGNCC 1 cut(s) 341
PsuI RGATCY 1 cut(s) 4
SaqAI TTAA 3 cut(s) 189, 404, 463
SatI GCNGC 5 cut(s) 99, 141, 209, 233, 453
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 198, 213, 222
SduI GDGCHC 1 cut(s) 445
SetI ASST 6 cut(s) 80, 103, 181, 314, 361, 457
SfaNI GCATC 2 cut(s) 44, 166
SmlI CTYRAG 2 cut(s) 102, 428
SmoI CTYRAG 2 cut(s) 102, 428
Sse9I AATT 1 cut(s) 369
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 3 cut(s) 141, 209, 233
SspMI CTAG 2 cut(s) 93, 152
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 196, 211, 220
TasI AATT 1 cut(s) 369
TauI GCSGC 3 cut(s) 143, 211, 235
Tru1I TTAA 3 cut(s) 189, 404, 463
Tru9I TTAA 3 cut(s) 189, 404, 463
TseI GCWGC 2 cut(s) 98, 452
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 2 cut(s) 93, 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.