pycom09g04190

WLM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004067
Physical Location & Seq
Forward (+)
3078564 .. 3080960
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1491 bp
ATGAGGCTAATTATTCCGCAGTTCTCGGATAAAAGTTCAAAAATGTTGTCTCCCTTCTCTGATGAGCATCAGAACCTGAGTTTACAGGAAGCTTCCATCATTGAGGGGAAGTCCATCAGAATGATGGGAGTGTCTGAAAACGAGGTTGATGAGGTTTTACAAAGTGCAAAGGCAAACTTAAGGATAGCTGGATTTGATGAAGAGGAAAAGAGACTGAGGCAGCGGATGACAGGCAGGCCTCAGAGTCTGAAACTTCCGCAAGGACCTTATATCTTTTGTGAATTCCAGACACTTCAACTTCCAGGAATTGAGTTGAACCCTCCGGCTTCAGAGGCGTTGAAAAGAATGCACATGCTTGCTGCAGATCCTGGAATTATTTCTGTCATGAACAAGCATCGTTGGCGTGTGGGAATCATGACTGAAATGGCCCCTGTTGGTTATGTTGGCATAAGTCCCAAATGCATCCTCGGCTTCAATAAGAACCATGGAGAGGAGATATCTCTGCGTCTTCGAACAGATGACCTCAAGGGTTTCAGGAAATATGAAAGTATCAAGAAAACTCTTCTGCATGAACTTGCTCACATGGTATATTCTGAACATGATGCGAACTTTTATGCTTTGGATAAACAGTTGAACCAAGAAGCTGAGAGTTTAGATTGGACGAGATCAACAAGTCACACACTGAGTGGAGTGCGGTACCCGGAACGGTATGAAGAAGACTTCATTGTTGGAGACAGAAGTAATTCTTCTCAGAAGCTTGGAGGAAATATATCCGATCGGCTGCCGAGCGCTCGTTCAGCTTCAGTGGTTGCTGCTTATCACCGTATGGCAACTGTTTCTCATGATAGTGTCTCTGTAGCACATGAAGAATCACATCCTGATGATTCCGGGTCAAATATGCAAAAAGAATCCAAACTTCAGAAGAACCCTGATATAGAGCCTGATCCTGATGCCCCGTCTGATAATCAGAACAAGTTTGAGCCCAGTCCTGATGATTCTCAGGGAACTAATGAATCATCCTCACAATTTACGGGAAGCGGGACCTTATTTGGAAAAGATTTCGGTGAGTCTATGATCTGTCAACCTGAGTTAGCGGGGACCGAGTCTCGTGAAGAACCAGATCCTGATAATATGGATATTAAGGAAAGCAGGGGTCAAGCAAGGAATAATGTGGATGAACCTGATCCTGATGACTTGGATGCAAAAGCAGATAATTTAGGATATGGTTCATTTGGAAATATTATAAGACCTAAACACGACAGTTCTTTGGTTACTGAAACCATCAAACATGAAGACAATCCAAGGAAGGCTTATTATGAACCTGATCCTGATGATTCTCAATCAAATGGAGTCACTCGGGCAGATCTTGATCCTGATACTAATTTGGTGCATCCACAGGATACATCCAGAATGCAGATTGATGAACCCGATCCATTTAAGGATCGATCAAGAGTTTCAGAGAATTCAAGACCCTGTTACAATTTTTTGTAA

Protein Analysis

497

Amino Acids

55.66

Weight (kDa)

4.97

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WLM PF08325 104 - 276 8.6e-47 WLM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1244
Acc65I GGTACC 1 cut(s) 696
AccB1I GGYRCC 1 cut(s) 696
AciI CCGC 6 cut(s) 17, 223, 257, 694, 1038, 1094
AclWI GGATC 8 cut(s) 359, 938, 1115, 1178, 1319, 1364, 1424, 1449
AcsI RAATTY 2 cut(s) 281, 1462
AcuI CTGAAG 3 cut(s) 312, 786, 902
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 1 cut(s) 698
AfeI AGCGCT 1 cut(s) 790
AfiI CCNNNNNNNGG 1 cut(s) 490
AflII CTTAAG 1 cut(s) 178
AgsI TTSAA 7 cut(s) 39, 296, 316, 340, 475, 634, 1467
AjnI CCWGG 2 cut(s) 301, 367
AluBI AGCT 5 cut(s) 92, 188, 644, 757, 800
AluI AGCT 5 cut(s) 92, 188, 644, 757, 800
Alw26I GTCTC 5 cut(s) 54, 205, 726, 856, 1110
AlwI GGATC 8 cut(s) 359, 938, 1115, 1178, 1319, 1364, 1424, 1449
AlwNI CAGNNNCTG 4 cut(s) 76, 247, 368, 1124
Ama87I CYCGRG 1 cut(s) 1356
Aor51HI AGCGCT 1 cut(s) 790
AoxI GGCC 2 cut(s) 236, 426
ApeKI GCWGC 4 cut(s) 220, 359, 781, 812
ApoI RAATTY 2 cut(s) 281, 1462
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
Asp700I GAANNNNTTC 2 cut(s) 376, 742
Asp718I GGTACC 1 cut(s) 696
AspLEI GCGC 1 cut(s) 791
AspS9I GGNCC 4 cut(s) 263, 427, 1041, 1098
AsuC2I CCSGG 2 cut(s) 701, 889
AsuHPI GGTGA 2 cut(s) 812, 1076
AsuII TTCGAA 1 cut(s) 511
AvaI CYCGRG 1 cut(s) 1356
AvaII GGWCC 3 cut(s) 263, 1041, 1098
BanI GGYRCC 1 cut(s) 696
BanII GRGCYC 1 cut(s) 984
BauI CACGAG 1 cut(s) 1107
BbsI GAAGAC 3 cut(s) 500, 723, 1299
BbvI GCAGC 4 cut(s) 232, 346, 768, 799
BccI CCATC 4 cut(s) 104, 118, 122, 1289
BciT130I CCWGG 2 cut(s) 303, 369
BciVI GTATCC 1 cut(s) 1393
BcnI CCSGG 2 cut(s) 701, 889
BcoDI GTCTC 5 cut(s) 54, 205, 726, 856, 1110
BfmI CTRYAG 2 cut(s) 360, 855
BfoI RGCGCY 1 cut(s) 792
BfrI CTTAAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 1393
BglII AGATCT 1 cut(s) 1363
BisI GCNGC 4 cut(s) 221, 360, 782, 813
BlsI GCNGC 4 cut(s) 222, 361, 783, 814
Bme1390I CCNGG 4 cut(s) 303, 369, 701, 889
Bme18I GGWCC 3 cut(s) 263, 1041, 1098
BmeT110I CYCGRG 1 cut(s) 1356
BmgT120I GGNCC 4 cut(s) 263, 427, 1041, 1098
BmiI GGNNCC 4 cut(s) 429, 698, 1042, 1099
BmrFI CCNGG 4 cut(s) 303, 369, 701, 889
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 7 cut(s) 76, 403, 471, 592, 940, 1189, 1399
BmuI ACTGGG 1 cut(s) 978
BpiI GAAGAC 3 cut(s) 500, 723, 1299
Bpu14I TTCGAA 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 509
BpuMI CCSGG 2 cut(s) 701, 889
Bsa29I ATCGAT 1 cut(s) 1444
BsaBI GATNNNNATC 2 cut(s) 66, 1368
BsaJI CCNNGG 3 cut(s) 466, 484, 1301
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse1I ACTGG 1 cut(s) 984
Bse8I GATNNNNATC 2 cut(s) 66, 1368
BseBI CCWGG 2 cut(s) 303, 369
BseCI ATCGAT 1 cut(s) 1444
BseDI CCNNGG 3 cut(s) 466, 484, 1301
BseGI GGATG 8 cut(s) 231, 462, 874, 1016, 1180, 1204, 1390, 1403
BseJI GATNNNNATC 2 cut(s) 66, 1368
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 8 cut(s) 68, 206, 254, 636, 674, 764, 1013, 1077
BseNI ACTGG 1 cut(s) 984
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 4 cut(s) 232, 346, 768, 799
Bsh1285I CGRYCG 1 cut(s) 778
BshFI GGCC 2 cut(s) 238, 428
BshNI GGYRCC 1 cut(s) 696
BshVI ATCGAT 1 cut(s) 1444
BsiEI CGRYCG 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 1356
BsiSI CCGG 3 cut(s) 323, 701, 888
BslFI GGGAC 3 cut(s) 438, 1054, 1111
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 5 cut(s) 54, 205, 726, 856, 1110
BsmFI GGGAC 3 cut(s) 438, 1054, 1111
BsmI GAATGC 2 cut(s) 351, 1416
BsnI GGCC 2 cut(s) 238, 428
BsoBI CYCGRG 1 cut(s) 1356
Bsp119I TTCGAA 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 984
Bsp19I CCATGG 1 cut(s) 484
BspACI CCGC 6 cut(s) 17, 223, 257, 694, 1038, 1094
BspANI GGCC 2 cut(s) 238, 428
BspCNI CTCAG 8 cut(s) 69, 207, 253, 637, 675, 763, 1012, 1078
BspDI ATCGAT 1 cut(s) 1444
BspHI TCATGA 3 cut(s) 384, 414, 841
BspLI GGNNCC 4 cut(s) 429, 698, 1042, 1099
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 8 cut(s) 359, 938, 1115, 1178, 1319, 1364, 1424, 1449
BspT104I TTCGAA 1 cut(s) 511
BspT107I GGYRCC 1 cut(s) 696
BspTI CTTAAG 1 cut(s) 178
BsrI ACTGG 1 cut(s) 984
BssECI CCNNGG 3 cut(s) 466, 484, 1301
BssSI CACGAG 1 cut(s) 1107
BssT1I CCWWGG 2 cut(s) 484, 1301
Bst2BI CACGAG 1 cut(s) 1107
Bst2UI CCWGG 2 cut(s) 303, 369
Bst4CI ACNGT 5 cut(s) 630, 708, 824, 835, 1262
Bst6I CTCTTC 2 cut(s) 195, 567
BstAFI CTTAAG 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 236, 357
BstDEI CTNAG 8 cut(s) 77, 215, 240, 645, 683, 750, 999, 1086
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 8 cut(s) 231, 462, 874, 1016, 1180, 1204, 1390, 1403
BstH2I RGCGCY 1 cut(s) 792
BstHHI GCGC 1 cut(s) 791
BstMAI GTCTC 5 cut(s) 54, 205, 726, 856, 1110
BstMCI CGRYCG 1 cut(s) 778
BstMWI GCNNNNNNNGC 4 cut(s) 332, 400, 468, 797
BstNI CCWGG 2 cut(s) 303, 369
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 4 cut(s) 301, 367, 699, 887
BstSFI CTRYAG 2 cut(s) 360, 855
BstV1I GCAGC 4 cut(s) 232, 346, 768, 799
BstV2I GAAGAC 3 cut(s) 500, 723, 1299
BstX2I RGATCY 3 cut(s) 364, 1120, 1363
BstYI RGATCY 3 cut(s) 364, 1120, 1363
Bsu15I ATCGAT 1 cut(s) 1444
BsuI GTATCC 1 cut(s) 1393
BsuRI GGCC 2 cut(s) 238, 428
BsuTUI ATCGAT 1 cut(s) 1444
BtgI CCRYGG 1 cut(s) 484
BtsCI GGATG 8 cut(s) 231, 462, 874, 1016, 1180, 1204, 1390, 1403
BtsIMutI CAGTG 2 cut(s) 680, 810
Cac8I GCNNGC 2 cut(s) 236, 357
CaiI CAGNNNCTG 4 cut(s) 76, 247, 368, 1124
CciI TCATGA 3 cut(s) 384, 414, 841
CfoI GCGC 1 cut(s) 791
Cfr13I GGNCC 4 cut(s) 263, 427, 1041, 1098
ClaI ATCGAT 1 cut(s) 1444
CseI GACGC 1 cut(s) 494
Csp6I GTAC 1 cut(s) 697
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 8 cut(s) 77, 215, 240, 645, 683, 750, 999, 1086
DraIII CACNNNGTG 1 cut(s) 686
Eam1104I CTCTTC 2 cut(s) 195, 567
EarI CTCTTC 2 cut(s) 195, 567
Eco130I CCWWGG 2 cut(s) 484, 1301
Eco147I AGGCCT 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 984
Eco32I GATATC 1 cut(s) 498
Eco47I GGWCC 3 cut(s) 263, 1041, 1098
Eco47III AGCGCT 1 cut(s) 790
Eco57I CTGAAG 3 cut(s) 312, 786, 902
Eco88I CYCGRG 1 cut(s) 1356
EcoO109I RGGNCCY 2 cut(s) 263, 1041
EcoRI GAATTC 2 cut(s) 281, 1462
EcoRII CCWGG 2 cut(s) 301, 367
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 2 cut(s) 484, 1301
EcoT22I ATGCAT 1 cut(s) 464
EcoT38I GRGCYC 1 cut(s) 984
ErhI CCWWGG 2 cut(s) 484, 1301
FalI AAGNNNNNCTT 6 cut(s) 161, 193, 730, 762, 1294, 1326
FaqI GGGAC 3 cut(s) 438, 1054, 1111
FauI CCCGC 2 cut(s) 1031, 1087
Fnu4HI GCNGC 4 cut(s) 221, 360, 782, 813
FokI GGATG 8 cut(s) 238, 449, 861, 1003, 1187, 1211, 1377, 1390
FriOI GRGCYC 1 cut(s) 984
Fsp4HI GCNGC 4 cut(s) 221, 360, 782, 813
GlaI GCGC 1 cut(s) 790
GluI GCNGC 4 cut(s) 221, 360, 782, 813
HaeII RGCGCY 1 cut(s) 792
HaeIII GGCC 2 cut(s) 238, 428
HapII CCGG 3 cut(s) 323, 701, 888
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 791
Hin6I GCGC 1 cut(s) 789
HinP1I GCGC 1 cut(s) 789
HincII GTYRAC 1 cut(s) 1082
HindII GTYRAC 1 cut(s) 1082
HindIII AAGCTT 2 cut(s) 90, 755
HpaII CCGG 3 cut(s) 323, 701, 888
HphI GGTGA 2 cut(s) 812, 1076
Hpy166II GTNNAC 2 cut(s) 83, 1082
Hpy8I GTNNAC 2 cut(s) 83, 1082
HpyAV CCTTC 2 cut(s) 64, 1300
HpyCH4III ACNGT 5 cut(s) 630, 708, 824, 835, 1262
HpyCH4V TGCA 9 cut(s) 167, 349, 362, 462, 568, 901, 1202, 1390, 1414
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 400, 468, 797
HpyF3I CTNAG 8 cut(s) 77, 215, 240, 645, 683, 750, 999, 1086
HspAI GCGC 1 cut(s) 789
KpnI GGTACC 1 cut(s) 700
Lsp1109I GCAGC 4 cut(s) 232, 346, 768, 799
LweI GCATC 7 cut(s) 76, 403, 471, 592, 940, 1189, 1399
MaeIII GTNAC 4 cut(s) 674, 1270, 1351, 1475
MflI RGATCY 3 cut(s) 364, 1120, 1363
MhlI GDGCHC 1 cut(s) 984
MlyI GAGTC 4 cut(s) 253, 1076, 1112, 1359
MmeI TCCRAC 1 cut(s) 709
Mph1103I ATGCAT 1 cut(s) 464
MroXI GAANNNNTTC 2 cut(s) 376, 742
MseI TTAA 3 cut(s) 179, 1140, 1437
MslI CAYNNNNRTG 3 cut(s) 119, 846, 879
MspA1I CMGCKG 1 cut(s) 223
MspCI CTTAAG 1 cut(s) 178
MspI CCGG 3 cut(s) 323, 701, 888
MspR9I CCNGG 4 cut(s) 303, 369, 701, 889
Mva1269I GAATGC 2 cut(s) 351, 1416
MvaI CCWGG 2 cut(s) 303, 369
MwoI GCNNNNNNNGC 4 cut(s) 332, 400, 468, 797
NciI CCSGG 2 cut(s) 701, 889
NcoI CCATGG 1 cut(s) 484
NlaIV GGNNCC 4 cut(s) 429, 698, 1042, 1099
NmeAIII GCCGAG 2 cut(s) 447, 810
NmuCI GTSAC 2 cut(s) 674, 1351
NsiI ATGCAT 1 cut(s) 464
NspI RCATGY 1 cut(s) 355
NspV TTCGAA 1 cut(s) 511
PagI TCATGA 3 cut(s) 384, 414, 841
PceI AGGCCT 1 cut(s) 238
PctI GAATGC 2 cut(s) 351, 1416
PdmI GAANNNNTTC 2 cut(s) 376, 742
PfeI GAWTC 7 cut(s) 411, 869, 884, 908, 995, 1013, 1334
PflFI GACNNNGTC 1 cut(s) 1102
PfoI TCCNGGA 2 cut(s) 301, 367
PkrI GCNGC 4 cut(s) 222, 361, 783, 814
Ple19I CGATCG 1 cut(s) 778
PleI GAGTC 4 cut(s) 252, 1075, 1111, 1358
PpsI GAGTC 4 cut(s) 252, 1075, 1111, 1358
PpuMI RGGWCCY 2 cut(s) 263, 1041
PsiI TTATAA 1 cut(s) 1244
Psp5II RGGWCCY 2 cut(s) 263, 1041
Psp6I CCWGG 2 cut(s) 301, 367
PspGI CCWGG 2 cut(s) 301, 367
PspN4I GGNNCC 4 cut(s) 429, 698, 1042, 1099
PspPI GGNCC 4 cut(s) 263, 427, 1041, 1098
PspPPI RGGWCCY 2 cut(s) 263, 1041
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 4 cut(s) 76, 247, 368, 1124
PsuI RGATCY 3 cut(s) 364, 1120, 1363
PsyI GACNNNGTC 1 cut(s) 1102
PvuI CGATCG 1 cut(s) 778
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
RseI CAYNNNNRTG 3 cut(s) 119, 846, 879
SaqAI TTAA 3 cut(s) 179, 1140, 1437
SatI GCNGC 4 cut(s) 221, 360, 782, 813
Sau96I GGNCC 4 cut(s) 263, 427, 1041, 1098
SchI GAGTC 4 cut(s) 253, 1076, 1112, 1359
ScrFI CCNGG 4 cut(s) 303, 369, 701, 889
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 7 cut(s) 76, 403, 471, 592, 940, 1189, 1399
SfcI CTRYAG 2 cut(s) 360, 855
SfuI TTCGAA 1 cut(s) 511
SinI GGWCC 3 cut(s) 263, 1041, 1098
SmiMI CAYNNNNRTG 3 cut(s) 119, 846, 879
SmlI CTYRAG 2 cut(s) 178, 524
SmoI CTYRAG 2 cut(s) 178, 524
SseBI AGGCCT 1 cut(s) 238
SsiI CCGC 6 cut(s) 17, 223, 257, 694, 1038, 1094
SspI AATATT 1 cut(s) 1240
StuI AGGCCT 1 cut(s) 238
StyD4I CCNGG 4 cut(s) 301, 367, 699, 887
StyI CCWWGG 2 cut(s) 484, 1301
TaaI ACNGT 5 cut(s) 630, 708, 824, 835, 1262
TaqI TCGA 2 cut(s) 511, 1444
TaqII GACCGA 1 cut(s) 1115
TfiI GAWTC 7 cut(s) 411, 869, 884, 908, 995, 1013, 1334
Tru1I TTAA 3 cut(s) 179, 1140, 1437
Tru9I TTAA 3 cut(s) 179, 1140, 1437
TscAI CASTG 2 cut(s) 687, 810
TseFI GTSAC 2 cut(s) 674, 1351
TseI GCWGC 4 cut(s) 220, 359, 781, 812
Tsp45I GTSAC 2 cut(s) 674, 1351
TspRI CASTG 2 cut(s) 687, 810
Tth111I GACNNNGTC 1 cut(s) 1102
Vha464I CTTAAG 1 cut(s) 178
VpaK11BI GGWCC 3 cut(s) 263, 1041, 1098
XapI RAATTY 2 cut(s) 281, 1462
XceI RCATGY 1 cut(s) 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmnI GAANNNNTTC 2 cut(s) 376, 742
Zsp2I ATGCAT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.