Rh2DG549900

WLM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004067
Physical Location & Seq
Reverse (-)
77865852 .. 77870980
5129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG549900.1

Sequence Viewer

Length: 1842 bp
ATGAGAAGTGTACACAACGTTGAAGTAATATGGAGAGGAAACAAGATTAATGTAGAGATCGATCCTGATGCAACTCTCAAGGAGCTCGGGCAGGAGCTGCAAAGATTGACTAATGTCAAAGCAGATACAATGAGGTTAATTGTGCCACAGTTGCCCAACAAAACCTCGAGATTGCTGTCTCCTTTCTCCGATGAGCATGAGCGCCTGAGTTTACAAGAAACTTCTATCCTTGAGGGCAAGTCCATTCGAATGATGGGAGCATTTGAGAATGAGGTCAACGAAGTTCTACAAAACGCAAAGGCAAACTTAAGGATAGCAGGATTTGATGAAGAGGAAAAGAGACTGAGGCAGAGAATGTCTGATAGGCCTCATACTTCCCTGAAACTTCCACAAGGACCTTATATCTTTAGTGATTTCCGCACACTTCAACTTCCAGGAATTGAGTTGAACCCTCCAGCATCCGAGGCCCTGAAAATAATGCACATGCTTGCTGCAGATCCTGGAATTGTTGCTATCATGAACAAGCATCGCTGGCATGTGGGAATCATGACTGAGATGGCCCCGGTCGGTTATGTTGGCATAAGTCCCAAATGCCTTCTCGGCTTCAACAAGAATAAGGGAGAGGAGATATCTCTGCGTCTTCGAACTGATGACCTTAAGGGTTTCCGGAAGTATGCAAGTATCAAGAAAACTCTTCTGCATGAGCTTGCTCACATGATATACTCTGAACATGATGCAACCTTTTATGCTTTTGATAAACAGTTGAATCAAGAAGCTGAGAGTTTAGATTGGACGAGATCAAGAGGTCACACACTAAGCGGAGCTAGGTATACAGAGCATTATGAAAATTCCTATGTTGGAGAGAGAGGTAATTCTTCTCACAAGCTTGGAGGAAATAAGCCTGATCAACTGGCCAGTGCCCGAGCATCTTCAGTTGCTGCTGCTTATCATAGACTGGCAACTGCTTCTGATGATAGCATCTCCGAGGTACATGAACAGTCACATCTTGATGGTTTTGCGTTCCATGTGCATGAAAAAGACAAGCATAAGAACTTTTCAGGTAAAAGGGACTTGGAGATTGAAAGTTCTTACAAAGTTCAAGGGCAAAATGATCTGGAGCCTGACCCTGATGACTGTTTTGGCAATGAGAACAAGTTTGAGCCCAGTCTTGATGATTCTCAAGGAACTGATGAATCTTGCTCAAAATCTACAGGAAGCAGGAAAAATGTGGAACCTGATCCTGATAATCTCGAATCTTGTTTGAAGTGTGATATCATAGCTGAACCTGAACCCTTACACCCTCAGGAGATGGAGATTTTGGAAAGCAGGTTTCAACCAAGGAATAATGTAGACGAACCTGATCCTGATGATTGTGATGTAAAGCGGGATGATTTGGGATCTAATTCAAATGGAAATATCACAAGACCTGGCAAGGATGAGTCTTTAGTTACTGAAACTACAAAATATGAAGCGAATTTGAGGAAGGCTTATGAAGAACCCGATCCTGATGCCTCTATGTCAAATGGCATTATTCAGCCAGTATCTAGATCTAATGATAATTTGGTGCTTCCAAATGAAATATCCAGTATGCAGATCGATGAACCAGATCCAGATGATCAAGAAATTCAAATAATTCAAGACCCTGTTTCAGTTGCTTGTAAACGTATGCAGAAGACCATTGAGATGTTGCAAGCTGAGGTTAATCCCACACAAGCTACCACAGTCCTCCAAACTCTATTTAAGATAATAAGGTACAATAACTTAATTTTGAGCATTGATTCATTCCTTGTCTTCATGAACACACAAAAACTATCAGGACTGTACATAACTGTAACTATATAA

Protein Analysis

613

Amino Acids

69.13

Weight (kDa)

5.14

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WLM PF08325 148 - 319 4.1e-48 WLM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1317
AccI GTMKAC 2 cut(s) 830, 1350
AccIII TCCGGA 1 cut(s) 666
AciI CCGC 3 cut(s) 418, 819, 1384
AclI AACGTT 1 cut(s) 18
AclWI GGATC 7 cut(s) 56, 491, 1232, 1355, 1405, 1496, 1601
AcoI YGGCCR 1 cut(s) 912
AcsI RAATTY 3 cut(s) 847, 1474, 1623
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 4 cut(s) 12, 990, 1754, 1823
AflII CTTAAG 2 cut(s) 307, 656
AjnI CCWGG 3 cut(s) 433, 499, 1426
AluBI AGCT 9 cut(s) 85, 97, 706, 776, 824, 886, 1280, 1694, 1715
AluI AGCT 9 cut(s) 85, 97, 706, 776, 824, 886, 1280, 1694, 1715
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 2 cut(s) 183, 334
AlwI GGATC 7 cut(s) 56, 491, 1232, 1355, 1405, 1496, 1601
AlwNI CAGNNNCTG 3 cut(s) 97, 500, 938
Ama87I CYCGRG 3 cut(s) 86, 166, 921
Aor13HI TCCGGA 1 cut(s) 666
AoxI GGCC 4 cut(s) 365, 465, 558, 912
ApeKI GCWGC 4 cut(s) 97, 491, 938, 941
ApoI RAATTY 3 cut(s) 847, 1474, 1623
AseI ATTAAT 1 cut(s) 48
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 3 cut(s) 395, 466, 559
AsuC2I CCSGG 1 cut(s) 563
AsuII TTCGAA 2 cut(s) 247, 643
AvaI CYCGRG 3 cut(s) 86, 166, 921
AvaII GGWCC 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 1302
BaeGI GKGCMC 1 cut(s) 922
BalI TGGCCA 1 cut(s) 914
BanII GRGCYC 2 cut(s) 87, 1164
BbsI GAAGAC 3 cut(s) 632, 1679, 1783
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 1695
BbvI GCAGC 4 cut(s) 84, 478, 925, 928
BccI CCATC 4 cut(s) 247, 550, 1004, 1303
BcgI CGANNNNNNTGC 4 cut(s) 50, 84, 1490, 1524
BciT130I CCWGG 3 cut(s) 435, 501, 1428
BclI TGATCA 2 cut(s) 904, 1615
BcnI CCSGG 1 cut(s) 563
BcoDI GTCTC 2 cut(s) 183, 334
BfaI CTAG 2 cut(s) 825, 1545
BfmI CTRYAG 2 cut(s) 492, 1209
BfoI RGCGCY 1 cut(s) 205
BfrI CTTAAG 2 cut(s) 307, 656
BfuAI ACCTGC 1 cut(s) 1317
BglI GCCNNNNNGGC 1 cut(s) 600
BglII AGATCT 1 cut(s) 1547
BisI GCNGC 4 cut(s) 98, 492, 939, 942
BlsI GCNGC 4 cut(s) 99, 493, 940, 943
Bme1390I CCNGG 4 cut(s) 435, 501, 563, 1428
Bme18I GGWCC 1 cut(s) 395
BmeT110I CYCGRG 3 cut(s) 86, 166, 921
BmgT120I GGNCC 3 cut(s) 395, 466, 559
BmiI GGNNCC 3 cut(s) 561, 1119, 1233
BmrFI CCNGG 4 cut(s) 435, 501, 563, 1428
BmrI ACTGGG 1 cut(s) 1158
BmsI GCATC 7 cut(s) 58, 467, 535, 724, 935, 987, 1498
BmuI ACTGGG 1 cut(s) 1158
BoxI GACNNNNGTC 1 cut(s) 113
BpiI GAAGAC 3 cut(s) 632, 1679, 1783
BpmI CTGGAG 2 cut(s) 438, 1136
Bpu10I CCTNAGC 1 cut(s) 1695
Bpu14I TTCGAA 2 cut(s) 247, 643
BpuEI CTTGAG 3 cut(s) 62, 251, 1164
BpuMI CCSGG 1 cut(s) 563
Bsa29I ATCGAT 2 cut(s) 60, 1596
BsaJI CCNNGG 4 cut(s) 462, 561, 984, 1337
BsaWI WCCGGW 1 cut(s) 666
Bse1I ACTGG 6 cut(s) 915, 915, 960, 1164, 1538, 1584
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 1 cut(s) 1150
BseAI TCCGGA 1 cut(s) 666
BseBI CCWGG 3 cut(s) 435, 501, 1428
BseCI ATCGAT 2 cut(s) 60, 1596
BseDI CCNNGG 4 cut(s) 462, 561, 984, 1337
BseGI GGATG 3 cut(s) 458, 1393, 1441
BseMI GCAATG 1 cut(s) 1150
BseMII CTCAG 6 cut(s) 197, 335, 543, 768, 1316, 1686
BseNI ACTGG 6 cut(s) 915, 915, 960, 1164, 1538, 1584
BseRI GAGGAG 1 cut(s) 638
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 4 cut(s) 84, 478, 925, 928
Bsh1285I CGRYCG 1 cut(s) 567
BshFI GGCC 4 cut(s) 367, 467, 560, 914
BshVI ATCGAT 2 cut(s) 60, 1596
BsiEI CGRYCG 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 3 cut(s) 86, 166, 921
BsiSI CCGG 2 cut(s) 563, 667
BslFI GGGAC 2 cut(s) 570, 1082
BsmAI GTCTC 2 cut(s) 183, 334
BsmFI GGGAC 2 cut(s) 570, 1082
BsnI GGCC 4 cut(s) 367, 467, 560, 914
BsoBI CYCGRG 3 cut(s) 86, 166, 921
Bsp119I TTCGAA 2 cut(s) 247, 643
Bsp1286I GDGCHC 3 cut(s) 87, 922, 1164
Bsp13I TCCGGA 1 cut(s) 666
Bsp1407I TGTACA 2 cut(s) 10, 1821
BspACI CCGC 3 cut(s) 418, 819, 1384
BspANI GGCC 4 cut(s) 367, 467, 560, 914
BspCNI CTCAG 6 cut(s) 198, 336, 544, 769, 1315, 1687
BspDI ATCGAT 2 cut(s) 60, 1596
BspEI TCCGGA 1 cut(s) 666
BspHI TCATGA 3 cut(s) 516, 546, 1794
BspLI GGNNCC 3 cut(s) 561, 1119, 1233
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 1317
BspPI GGATC 7 cut(s) 56, 491, 1232, 1355, 1405, 1496, 1601
BspT104I TTCGAA 2 cut(s) 247, 643
BspTI CTTAAG 2 cut(s) 307, 656
BsrDI GCAATG 1 cut(s) 1150
BsrGI TGTACA 2 cut(s) 10, 1821
BsrI ACTGG 6 cut(s) 915, 915, 960, 1164, 1538, 1584
BssECI CCNNGG 4 cut(s) 462, 561, 984, 1337
BssNAI GTATAC 1 cut(s) 831
BssT1I CCWWGG 1 cut(s) 1337
Bst1107I GTATAC 1 cut(s) 831
Bst2UI CCWGG 3 cut(s) 435, 501, 1428
Bst4CI ACNGT 7 cut(s) 150, 762, 999, 1136, 1723, 1821, 1831
Bst6I CTCTTC 2 cut(s) 324, 699
BstAFI CTTAAG 2 cut(s) 307, 656
BstAPI GCANNNNNTGC 1 cut(s) 97
BstAUI TGTACA 2 cut(s) 10, 1821
BstBI TTCGAA 2 cut(s) 247, 643
BstC8I GCNNGC 4 cut(s) 489, 533, 708, 1692
BstDEI CTNAG 7 cut(s) 206, 344, 552, 777, 815, 1302, 1695
BstF5I GGATG 3 cut(s) 458, 1393, 1441
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstMAI GTCTC 2 cut(s) 183, 334
BstMCI CGRYCG 1 cut(s) 567
BstMWI GCNNNNNNNGC 5 cut(s) 97, 151, 464, 532, 600
BstNI CCWGG 3 cut(s) 435, 501, 1428
BstNSI RCATGY 2 cut(s) 487, 539
BstPAI GACNNNNGTC 1 cut(s) 113
BstSCI CCNGG 4 cut(s) 433, 499, 561, 1426
BstSFI CTRYAG 2 cut(s) 492, 1209
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 4 cut(s) 84, 478, 925, 928
BstV2I GAAGAC 3 cut(s) 632, 1679, 1783
BstX2I RGATCY 4 cut(s) 496, 1397, 1547, 1606
BstYI RGATCY 4 cut(s) 496, 1397, 1547, 1606
BstZ17I GTATAC 1 cut(s) 831
Bsu15I ATCGAT 2 cut(s) 60, 1596
Bsu36I CCTNAGG 1 cut(s) 1302
BsuRI GGCC 4 cut(s) 367, 467, 560, 914
BsuTUI ATCGAT 2 cut(s) 60, 1596
BtgZI GCGATG 1 cut(s) 512
BtsCI GGATG 3 cut(s) 458, 1393, 1441
BtsIMutI CAGTG 1 cut(s) 922
BveI ACCTGC 1 cut(s) 1317
Cac8I GCNNGC 4 cut(s) 489, 533, 708, 1692
CaiI CAGNNNCTG 3 cut(s) 97, 500, 938
CciI TCATGA 3 cut(s) 516, 546, 1794
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 395, 466, 559
ClaI ATCGAT 2 cut(s) 60, 1596
CseI GACGC 1 cut(s) 626
Csp6I GTAC 4 cut(s) 11, 989, 1753, 1822
CviQI GTAC 4 cut(s) 11, 989, 1753, 1822
DdeI CTNAG 7 cut(s) 206, 344, 552, 777, 815, 1302, 1695
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 2 cut(s) 324, 699
EarI CTCTTC 2 cut(s) 324, 699
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 1337
Eco147I AGGCCT 1 cut(s) 367
Eco24I GRGCYC 2 cut(s) 87, 1164
Eco32I GATATC 2 cut(s) 630, 1273
Eco47I GGWCC 1 cut(s) 395
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 915
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 3 cut(s) 86, 166, 921
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 2 cut(s) 395, 466
EcoRII CCWGG 3 cut(s) 433, 499, 1426
EcoRV GATATC 2 cut(s) 630, 1273
EcoT14I CCWWGG 1 cut(s) 1337
EcoT38I GRGCYC 2 cut(s) 87, 1164
ErhI CCWWGG 1 cut(s) 1337
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 2 cut(s) 570, 1082
FauI CCCGC 1 cut(s) 1377
FbaI TGATCA 2 cut(s) 904, 1615
FblI GTMKAC 2 cut(s) 830, 1350
Fnu4HI GCNGC 4 cut(s) 98, 492, 939, 942
FokI GGATG 3 cut(s) 445, 1400, 1448
FriOI GRGCYC 2 cut(s) 87, 1164
Fsp4HI GCNGC 4 cut(s) 98, 492, 939, 942
FspBI CTAG 2 cut(s) 825, 1545
GlaI GCGC 1 cut(s) 203
GluI GCNGC 4 cut(s) 98, 492, 939, 942
GsuI CTGGAG 2 cut(s) 438, 1136
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 4 cut(s) 367, 467, 560, 914
HapII CCGG 2 cut(s) 563, 667
HgaI GACGC 1 cut(s) 626
HhaI GCGC 1 cut(s) 204
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 7 cut(s) 543, 766, 1175, 1193, 1253, 1439, 1778
HpaII CCGG 2 cut(s) 563, 667
Hpy166II GTNNAC 7 cut(s) 11, 13, 212, 277, 831, 1351, 1661
Hpy188I TCNGA 6 cut(s) 190, 361, 463, 727, 970, 985
Hpy8I GTNNAC 7 cut(s) 11, 13, 212, 277, 831, 1351, 1661
HpyAV CCTTC 2 cut(s) 605, 1477
HpyCH4III ACNGT 7 cut(s) 150, 762, 999, 1136, 1723, 1821, 1831
HpyCH4IV ACGT 2 cut(s) 18, 1663
HpyF10VI GCNNNNNNNGC 5 cut(s) 97, 151, 464, 532, 600
HpyF3I CTNAG 7 cut(s) 206, 344, 552, 777, 815, 1302, 1695
HpySE526I ACGT 2 cut(s) 18, 1663
HspAI GCGC 1 cut(s) 202
Kpn2I TCCGGA 1 cut(s) 666
Ksp22I TGATCA 2 cut(s) 904, 1615
LmnI GCTCC 5 cut(s) 82, 94, 257, 821, 1117
Lsp1109I GCAGC 4 cut(s) 84, 478, 925, 928
LweI GCATC 7 cut(s) 58, 467, 535, 724, 935, 987, 1498
MaeI CTAG 2 cut(s) 825, 1545
MaeII ACGT 2 cut(s) 18, 1663
MaeIII GTNAC 4 cut(s) 806, 999, 1447, 1831
MboII GAAGA 8 cut(s) 341, 632, 686, 867, 921, 1505, 1684, 1783
MflI RGATCY 4 cut(s) 496, 1397, 1547, 1606
MhlI GDGCHC 3 cut(s) 87, 922, 1164
MlsI TGGCCA 1 cut(s) 914
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 1 cut(s) 1448
MmeI TCCRAC 1 cut(s) 838
Mox20I TGGCCA 1 cut(s) 914
MroI TCCGGA 1 cut(s) 666
MscI TGGCCA 1 cut(s) 914
MseI TTAA 7 cut(s) 48, 137, 308, 657, 1701, 1740, 1763
MslI CAYNNNNRTG 4 cut(s) 248, 1008, 1029, 1682
Msp20I TGGCCA 1 cut(s) 914
MspCI CTTAAG 2 cut(s) 307, 656
MspI CCGG 2 cut(s) 563, 667
MspR9I CCNGG 4 cut(s) 435, 501, 563, 1428
MvaI CCWGG 3 cut(s) 435, 501, 1428
MwoI GCNNNNNNNGC 5 cut(s) 97, 151, 464, 532, 600
NciI CCSGG 1 cut(s) 563
NlaIV GGNNCC 3 cut(s) 561, 1119, 1233
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 2 cut(s) 806, 999
NspI RCATGY 2 cut(s) 487, 539
NspV TTCGAA 2 cut(s) 247, 643
PaeR7I CTCGAG 1 cut(s) 166
PagI TCATGA 3 cut(s) 516, 546, 1794
PceI AGGCCT 1 cut(s) 367
PfeI GAWTC 6 cut(s) 543, 766, 1175, 1193, 1253, 1778
PfoI TCCNGGA 2 cut(s) 433, 499
PkrI GCNGC 4 cut(s) 99, 493, 940, 943
PleI GAGTC 1 cut(s) 1447
PpsI GAGTC 1 cut(s) 1447
PpuMI RGGWCCY 1 cut(s) 395
PshAI GACNNNNGTC 1 cut(s) 113
PshBI ATTAAT 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 3 cut(s) 433, 499, 1426
PspGI CCWGG 3 cut(s) 433, 499, 1426
PspN4I GGNNCC 3 cut(s) 561, 1119, 1233
PspPI GGNCC 3 cut(s) 395, 466, 559
PspPPI RGGWCCY 1 cut(s) 395
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 3 cut(s) 97, 500, 938
PsuI RGATCY 4 cut(s) 496, 1397, 1547, 1606
RsaI GTAC 4 cut(s) 12, 990, 1754, 1823
RsaNI GTAC 4 cut(s) 11, 989, 1753, 1822
RseI CAYNNNNRTG 4 cut(s) 248, 1008, 1029, 1682
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 7 cut(s) 48, 137, 308, 657, 1701, 1740, 1763
SatI GCNGC 4 cut(s) 98, 492, 939, 942
Sau96I GGNCC 3 cut(s) 395, 466, 559
SchI GAGTC 1 cut(s) 1448
ScrFI CCNGG 4 cut(s) 435, 501, 563, 1428
SduI GDGCHC 3 cut(s) 87, 922, 1164
SfaNI GCATC 7 cut(s) 58, 467, 535, 724, 935, 987, 1498
SfcI CTRYAG 2 cut(s) 492, 1209
Sfr274I CTCGAG 1 cut(s) 166
SfuI TTCGAA 2 cut(s) 247, 643
SinI GGWCC 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 166
SmiMI CAYNNNNRTG 4 cut(s) 248, 1008, 1029, 1682
SmlI CTYRAG 6 cut(s) 77, 166, 230, 307, 656, 1179
SmoI CTYRAG 6 cut(s) 77, 166, 230, 307, 656, 1179
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 3 cut(s) 418, 819, 1384
SspMI CTAG 2 cut(s) 825, 1545
SstI GAGCTC 1 cut(s) 87
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 4 cut(s) 433, 499, 561, 1426
StyI CCWWGG 1 cut(s) 1337
TaaI ACNGT 7 cut(s) 150, 762, 999, 1136, 1723, 1821, 1831
TaiI ACGT 2 cut(s) 21, 1666
TaqI TCGA 6 cut(s) 60, 167, 247, 643, 1251, 1596
TatI WGTACW 2 cut(s) 10, 1821
TfiI GAWTC 6 cut(s) 543, 766, 1175, 1193, 1253, 1778
Tru1I TTAA 7 cut(s) 48, 137, 308, 657, 1701, 1740, 1763
Tru9I TTAA 7 cut(s) 48, 137, 308, 657, 1701, 1740, 1763
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 2 cut(s) 806, 999
TseI GCWGC 4 cut(s) 97, 491, 938, 941
Tsp45I GTSAC 2 cut(s) 806, 999
TspRI CASTG 1 cut(s) 922
Vha464I CTTAAG 2 cut(s) 307, 656
VpaK11BI GGWCC 1 cut(s) 395
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 3 cut(s) 847, 1474, 1623
XbaI TCTAGA 1 cut(s) 1544
XceI RCATGY 2 cut(s) 487, 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 166
XmiI GTMKAC 2 cut(s) 830, 1350
XspI CTAG 2 cut(s) 825, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.