Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004067

Physical Location & Seq

Genomic Coordinates Reverse (-)

70970607 .. 70977357

Gene Structure 6751 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021001

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGACTAGAGCTTCCAAAACAACGACAAAGAGAAGCACTACTGTTACTATTTTCTGGGTAGCCGTTGTCGTGGTGATTATGTTGTCGATTGTGGTTGAAGAAGTAGAGGCGGGGAGGCCGGACCACCGTCTGAAGAGTGCAGATGATGACGACGACGACGATGATAACTATGAGATCAATAGCAAGCACTACATTAACAATAAGGTTAATACCTTTATTAAACGAGGACGTCTGATTGTTATGAAATTGTCTCGAGGTGAATTACTGCTGCAAGGGAATGCTATAGAGGACAACATGAGAAGTGTACACAACGTTGAAGTAATATGGAGAGGAAACAAGATTACTGTAGAGATCAATCCTGATGCAACTCTCAAGGAGCTCGGGCAGGAGCTGCAAAGATTAACTAATGTCAAAGCAGATACAATGAGGTTAATTGTGCCACAGTTGCCCAATAAAACCTCGAGATTGCTGTCTCCTTTCTCGGATGAGCATGAGCGCCTGAGTTTACAACAAACTTCTATCCTTGAGGGCAAGTCCATTAGAATGATGGGAGCATTTGAGAATGAGGTCGACGAAGTTCTACAAAACGCAAAGGCAAACTTAAGGATAGCAGGATTTGATGAGGAGGAAAAGAGACTGAGACAGAGACTGTCTGATAGGCCTCATACTTCCCTGAAACTTCCACAAGGACCTTATATCTTTAGTGATTTCCGCACGCTTCAACTTCCAGGAATTGAGTTGGACCCTCCAGCATCCGAGGCCCTGAAAATAATGCACATGCTTGCTGCAGATCCTGGAATTGTTGCTATCATGAACAAGCATCGCTGGCATGTGGGAATCATGACTGAGATGGCCCCGGTTGGTTATGTTGGCATAAGTCCCAAATGCCTTCTCGGCTTCAACAAGAATAAGGGAGAGGAGATATCTCTGCGTCTTCGAACTGATGACCTCAAGGGTTTCCGGAAGTATGCAAGTATCAAGAAAACTCTTCTGCATGAGCTTGCTCACATGATATACTCTGAACATGATGCAACCTTTTATGCTTTTGATAAACAGTTGAATCAAGAAGCTGAGAGTTTAGATTGGACGAGATCAAGAGGTCACACACTAAGCGCAGCTAGGCATACAGAGCATTATGAAAATTCCTATGTTGGAGAGAGAAGTAATTCTTCTCACAAGCTTGGAGGAAATATGCCTGATCAACTGGCCAGTGCCCGTGCATCTTCAGTTGCTGCTGCTTATCATAGACTTGCAACTGCTTCTGATGATAGCATCTCCAAGGTACATGAACAGTCACGTCTTGATGGTTTTGCGTTCCATGTGCATGAAAAAGACGAGCATAAGAACTTTTCAGGTAAAAGAGACTTGGAGATTGAAAGTTCTTACAAAGTTCAAAGGCAAAATGATCTGGAGCCTGACCCTGATGACTGTTTTGGCAATGAGAATAAGTTTGAGCCCAGTCTTGATGATTCTCAAGGACCTGATGAATCTTGCTCAAAATCTACAGGAAGCAGGAAAAATGTGGAATCTGATCCTGATAATCTTGAAGCTTGTTTGAAGTGTGATATCATAGCTGAACCTGAACCCTTAAACCCTCAGGAGATGGAGATTTTGGAAAGCAGGTTTCAACCAAGGAATAATTTAGACGAACCTGATCCTGATGATTATGATGCAAAGCGGGATGATTTGGGATCTAATTCAAACGGAAATATCACAAGACCTGGCAAGGATGAGTCTTTAGTTACTGAAACTACAAAATATGAAAAGAATTTGAGGAAGGCTTATGAAGAACCCGATCCTGATGCCTCGATGTCAAATGGCATTATTCAGCCACTATCTAGATCTAATGATAATTTGGTGCTTCCAAATGAAATATCCAGTATGCAGATCGATGAACCAGATCCAGATGATCAAGAGATTCAAATAATTCAAGACCCTGTTTCAGTTGCTTGTAAACGTATGCAGAAGACCATTGAGATGTTGCAAGCTGAGGTTAATCCCACACAAGCTACCACAGTCCTCCAAACTCTATTTAAGATAATAAGGAATGTTCTTGAACACCCAGGTGAATCGAAATACAGAAGACTGCGCAAGGCTAATCCCATGATTCAAAAGAATGTAGCAAACTACAAAGCTGCCATGGAATTTCTGTTTCTGATTGGCTTCAATGAAAATGTTGTGGATGAAATTGGACGACCAGAAACTTATCTAGTGTTGAAGCGGGATGATCCGGGCTTATTGTGGCTTGCCAAGTCTTCCCTGGAGACATGTATTACTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009987 GO:0016787 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0032446 GO:0033554 GO:0036211 GO:0043167 GO:0043169 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044877 GO:0046872 GO:0046914 GO:0050896 GO:0051716 GO:0070011 GO:0070628 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUB WLM

Protein Analysis

761

Amino Acids

86.06

Weight (kDa)

5.32

Isoelectric Point (pI)

48.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WLM	PF08325	247 - 417	1.2e-46	WLM domain
PUB	PF09409	669 - 745	1.5e-18	PUB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35690
fragaria_vesca	FvH4_6g41820 FvH4_6g41820 FvH4_6g41820 FvH4_6g41820 FvH4_6g41820 FvH4_6g41820 FvH4_6g41820 FvH4_6g41820
malus_domestica	MD09G1117000.v1.1 MD17G1108500.v1.1
prunus_persica	Prupe.3G206900_v2.0.a1 Prupe.3G206900_v2.0.a1 Prupe.3G206900_v2.0.a1 Prupe.3G206900_v2.0.a1
pyrus_communis	pycom09g04190 pycom09g04200 pycom17g10100
rosa_chinensis	RchiOBHm_Chr2g0157621
rosa_laevigata	RLG00000021001 RLG00000021004
rosa_multiflora	Rmu_co8368661.1_g000001 Rmu_sc0000195.1_g000050 Rmu_sc0005255.1_g000015 Rmu_sc0006475.1_g000002 Rmu_sc0015282.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092080
rosa_rugosa	Rorug02G0462400
rosa_samantha	Rh2AG526500 Rh2BG541400 Rh2CG511200 Rh2DG549900
rosa_wichuraiana	Rw2G043610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	232
Acc16I	TGCGCA	1 cut(s)	2090
Acc36I	ACCTGC	1 cut(s)	1611
AccI	GTMKAC	1 cut(s)	570
AccIII	TCCGGA	1 cut(s)	960
AciI	CCGC	4 cut(s)	110, 712, 1678, 2221
AclI	AACGTT	1 cut(s)	312
AclWI	GGATC	7 cut(s)	785, 1526, 1649, 1699, 1790, 1895, 2222
AcoI	YGGCCR	1 cut(s)	1206
AcsI	RAATTY	3 cut(s)	1141, 1768, 2144
AcuI	CTGAAG	2 cut(s)	152, 1209
AcyI	GRCGYC	1 cut(s)	229
AfaI	GTAC	2 cut(s)	306, 1284
AflII	CTTAAG	1 cut(s)	601
AflIII	ACRYGT	1 cut(s)	2267
AjiI	CACGTC	1 cut(s)	1298
AjnI	CCWGG	5 cut(s)	727, 793, 1720, 2062, 2259
AleI	CACNNNNGTG	1 cut(s)	2064
Alw21I	GWGCWC	1 cut(s)	381
Alw26I	GTCTC	7 cut(s)	255, 477, 628, 634, 640, 1356, 2258
AlwI	GGATC	7 cut(s)	785, 1526, 1649, 1699, 1790, 1895, 2222
AlwNI	CAGNNNCTG	4 cut(s)	391, 649, 794, 1232
Ama87I	CYCGRG	3 cut(s)	252, 380, 460
Aor13HI	TCCGGA	1 cut(s)	960
AoxI	GGCC	5 cut(s)	116, 659, 759, 852, 1206
ApeKI	GCWGC	7 cut(s)	268, 391, 785, 1115, 1232, 1235, 2135
ApoI	RAATTY	3 cut(s)	1141, 1768, 2144
Asp700I	GAANNNNTTC	1 cut(s)	1165
AspLEI	GCGC	3 cut(s)	498, 1115, 2091
AspS9I	GGNCC	6 cut(s)	121, 689, 742, 760, 853, 1478
AsuC2I	CCSGG	2 cut(s)	857, 2232
AsuHPI	GGTGA	3 cut(s)	85, 269, 2078
AsuII	TTCGAA	1 cut(s)	937
AvaI	CYCGRG	3 cut(s)	252, 380, 460
AvaII	GGWCC	4 cut(s)	121, 689, 742, 1478
AxyI	CCTNAGG	1 cut(s)	1596
BaeGI	GKGCMC	1 cut(s)	1216
BalI	TGGCCA	1 cut(s)	1208
BanII	GRGCYC	2 cut(s)	381, 1458
BbsI	GAAGAC	4 cut(s)	926, 1973, 2089, 2247
Bbv12I	GWGCWC	1 cut(s)	381
BbvCI	CCTCAGC	1 cut(s)	1989
BbvI	GCAGC	7 cut(s)	255, 378, 772, 1127, 1219, 1222, 2122
BccI	CCATC	4 cut(s)	541, 844, 1298, 1597
BceAI	ACGGC	1 cut(s)	47
BcgI	CGANNNNNNTGC	2 cut(s)	1784, 1818
BciT130I	CCWGG	5 cut(s)	729, 795, 1722, 2064, 2261
BclI	TGATCA	2 cut(s)	1198, 1909
BcnI	CCSGG	2 cut(s)	857, 2232
BcoDI	GTCTC	7 cut(s)	255, 477, 628, 634, 640, 1356, 2258
BfaI	CTAG	4 cut(s)	6, 1119, 1839, 2210
BfmI	CTRYAG	4 cut(s)	282, 345, 786, 1503
BfoI	RGCGCY	1 cut(s)	499
BfrI	CTTAAG	1 cut(s)	601
BfuAI	ACCTGC	1 cut(s)	1611
BglI	GCCNNNNNGGC	1 cut(s)	894
BglII	AGATCT	1 cut(s)	1841
BisI	GCNGC	7 cut(s)	269, 392, 786, 1116, 1233, 1236, 2136
BlsI	GCNGC	7 cut(s)	270, 393, 787, 1117, 1234, 1237, 2137
Bme1390I	CCNGG	7 cut(s)	729, 795, 857, 1722, 2064, 2232, 2261
Bme18I	GGWCC	4 cut(s)	121, 689, 742, 1478
BmeT110I	CYCGRG	3 cut(s)	252, 380, 460
BmgBI	CACGTC	1 cut(s)	1298
BmgT120I	GGNCC	6 cut(s)	121, 689, 742, 760, 853, 1478
BmiI	GGNNCC	3 cut(s)	744, 855, 1413
BmrFI	CCNGG	7 cut(s)	729, 795, 857, 1722, 2064, 2232, 2261
BmrI	ACTGGG	1 cut(s)	1452
BmsI	GCATC	8 cut(s)	352, 761, 829, 1018, 1229, 1281, 1660, 1792
BmuI	ACTGGG	1 cut(s)	1452
BoxI	GACNNNNGTC	1 cut(s)	126
BpiI	GAAGAC	4 cut(s)	926, 1973, 2089, 2247
BpmI	CTGGAG	3 cut(s)	732, 1430, 2282
Bpu10I	CCTNAGC	1 cut(s)	1989
Bpu14I	TTCGAA	1 cut(s)	937
BpuEI	CTTGAG	4 cut(s)	356, 545, 935, 1458
BpuMI	CCSGG	2 cut(s)	857, 2232
Bsa29I	ATCGAT	1 cut(s)	1890
BsaHI	GRCGYC	1 cut(s)	229
BsaJI	CCNNGG	7 cut(s)	756, 855, 1278, 1631, 2062, 2139, 2259
BsaWI	WCCGGW	1 cut(s)	960
Bse1I	ACTGG	4 cut(s)	1209, 1209, 1458, 1878
Bse21I	CCTNAGG	1 cut(s)	1596
Bse3DI	GCAATG	1 cut(s)	1444
BseAI	TCCGGA	1 cut(s)	960
BseBI	CCWGG	5 cut(s)	729, 795, 1722, 2064, 2261
BseCI	ATCGAT	1 cut(s)	1890
BseDI	CCNNGG	7 cut(s)	756, 855, 1278, 1631, 2062, 2139, 2259
BseGI	GGATG	6 cut(s)	490, 752, 1687, 1735, 2188, 2230
BseMI	GCAATG	1 cut(s)	1444
BseMII	CTCAG	6 cut(s)	491, 629, 837, 1062, 1610, 1980
BseNI	ACTGG	4 cut(s)	1209, 1209, 1458, 1878
BseRI	GAGGAG	2 cut(s)	638, 932
BseSI	GKGCMC	1 cut(s)	1216
BseXI	GCAGC	7 cut(s)	255, 378, 772, 1127, 1219, 1222, 2122
BsgI	GTGCAG	1 cut(s)	159
BshFI	GGCC	5 cut(s)	118, 661, 761, 854, 1208
BshVI	ATCGAT	1 cut(s)	1890
BsiHKAI	GWGCWC	1 cut(s)	381
BsiHKCI	CYCGRG	3 cut(s)	252, 380, 460
BsiSI	CCGG	4 cut(s)	119, 857, 961, 2231
BslFI	GGGAC	1 cut(s)	864
BsmAI	GTCTC	7 cut(s)	255, 477, 628, 634, 640, 1356, 2258
BsmFI	GGGAC	1 cut(s)	864
BsmI	GAATGC	1 cut(s)	283
BsnI	GGCC	5 cut(s)	118, 661, 761, 854, 1208
BsoBI	CYCGRG	3 cut(s)	252, 380, 460
Bsp119I	TTCGAA	1 cut(s)	937
Bsp1286I	GDGCHC	3 cut(s)	381, 1216, 1458
Bsp13I	TCCGGA	1 cut(s)	960
Bsp1407I	TGTACA	1 cut(s)	304
Bsp19I	CCATGG	1 cut(s)	2139
BspACI	CCGC	4 cut(s)	110, 712, 1678, 2221
BspANI	GGCC	5 cut(s)	118, 661, 761, 854, 1208
BspCNI	CTCAG	6 cut(s)	492, 630, 838, 1063, 1609, 1981
BspDI	ATCGAT	1 cut(s)	1890
BspEI	TCCGGA	1 cut(s)	960
BspHI	TCATGA	2 cut(s)	810, 840
BspLI	GGNNCC	3 cut(s)	744, 855, 1413
BspMAI	CTGCAG	1 cut(s)	790
BspMI	ACCTGC	1 cut(s)	1611
BspPI	GGATC	7 cut(s)	785, 1526, 1649, 1699, 1790, 1895, 2222
BspT104I	TTCGAA	1 cut(s)	937
BspTI	CTTAAG	1 cut(s)	601
BsrDI	GCAATG	1 cut(s)	1444
BsrGI	TGTACA	1 cut(s)	304
BsrI	ACTGG	4 cut(s)	1209, 1209, 1458, 1878
BssECI	CCNNGG	7 cut(s)	756, 855, 1278, 1631, 2062, 2139, 2259
BssNI	GRCGYC	1 cut(s)	229
BssT1I	CCWWGG	3 cut(s)	1278, 1631, 2139
Bst2UI	CCWGG	5 cut(s)	729, 795, 1722, 2064, 2261
Bst6I	CTCTTC	2 cut(s)	128, 993
BstACI	GRCGYC	1 cut(s)	229
BstAFI	CTTAAG	1 cut(s)	601
BstAPI	GCANNNNNTGC	1 cut(s)	391
BstAUI	TGTACA	1 cut(s)	304
BstBI	TTCGAA	1 cut(s)	937
BstC8I	GCNNGC	7 cut(s)	185, 716, 783, 827, 1002, 1986, 2247
BstDEI	CTNAG	7 cut(s)	500, 638, 846, 1071, 1109, 1596, 1989
BstDSI	CCRYGG	1 cut(s)	2139
BstF5I	GGATG	6 cut(s)	490, 752, 1687, 1735, 2188, 2230
BstH2I	RGCGCY	1 cut(s)	499
BstHHI	GCGC	3 cut(s)	498, 1115, 2091
BstMAI	GTCTC	7 cut(s)	255, 477, 628, 634, 640, 1356, 2258
BstMWI	GCNNNNNNNGC	6 cut(s)	391, 445, 758, 826, 894, 1129
BstNI	CCWGG	5 cut(s)	729, 795, 1722, 2064, 2261
BstNSI	RCATGY	3 cut(s)	781, 833, 2271
BstPAI	GACNNNNGTC	1 cut(s)	126
BstSCI	CCNGG	7 cut(s)	727, 793, 855, 1720, 2062, 2230, 2259
BstSFI	CTRYAG	4 cut(s)	282, 345, 786, 1503
BstSLI	GKGCMC	1 cut(s)	1216
BstV1I	GCAGC	7 cut(s)	255, 378, 772, 1127, 1219, 1222, 2122
BstV2I	GAAGAC	4 cut(s)	926, 1973, 2089, 2247
BstX2I	RGATCY	4 cut(s)	790, 1691, 1841, 1900
BstYI	RGATCY	4 cut(s)	790, 1691, 1841, 1900
Bsu15I	ATCGAT	1 cut(s)	1890
Bsu36I	CCTNAGG	1 cut(s)	1596
BsuRI	GGCC	5 cut(s)	118, 661, 761, 854, 1208
BsuTUI	ATCGAT	1 cut(s)	1890
BtgI	CCRYGG	1 cut(s)	2139
BtgZI	GCGATG	1 cut(s)	806
BtrI	CACGTC	1 cut(s)	1298
BtsCI	GGATG	6 cut(s)	490, 752, 1687, 1735, 2188, 2230
BtsIMutI	CAGTG	1 cut(s)	1216
BveI	ACCTGC	1 cut(s)	1611
Cac8I	GCNNGC	7 cut(s)	185, 716, 783, 827, 1002, 1986, 2247
CaiI	CAGNNNCTG	4 cut(s)	391, 649, 794, 1232
CciI	TCATGA	2 cut(s)	810, 840
CfoI	GCGC	3 cut(s)	498, 1115, 2091
Cfr13I	GGNCC	6 cut(s)	121, 689, 742, 760, 853, 1478
ClaI	ATCGAT	1 cut(s)	1890
CseI	GACGC	1 cut(s)	920
Csp6I	GTAC	2 cut(s)	305, 1283
CviQI	GTAC	2 cut(s)	305, 1283
DdeI	CTNAG	7 cut(s)	500, 638, 846, 1071, 1109, 1596, 1989
EaeI	YGGCCR	1 cut(s)	1206
Eam1104I	CTCTTC	2 cut(s)	128, 993
EarI	CTCTTC	2 cut(s)	128, 993
Ecl136II	GAGCTC	1 cut(s)	379
Eco130I	CCWWGG	3 cut(s)	1278, 1631, 2139
Eco147I	AGGCCT	1 cut(s)	661
Eco24I	GRGCYC	2 cut(s)	381, 1458
Eco32I	GATATC	2 cut(s)	924, 1567
Eco47I	GGWCC	4 cut(s)	121, 689, 742, 1478
Eco53kI	GAGCTC	1 cut(s)	379
Eco57I	CTGAAG	2 cut(s)	152, 1209
Eco81I	CCTNAGG	1 cut(s)	1596
Eco88I	CYCGRG	3 cut(s)	252, 380, 460
EcoICRI	GAGCTC	1 cut(s)	379
EcoO109I	RGGNCCY	3 cut(s)	689, 760, 1478
EcoRII	CCWGG	5 cut(s)	727, 793, 1720, 2062, 2259
EcoRV	GATATC	2 cut(s)	924, 1567
EcoT14I	CCWWGG	3 cut(s)	1278, 1631, 2139
EcoT38I	GRGCYC	2 cut(s)	381, 1458
ErhI	CCWWGG	3 cut(s)	1278, 1631, 2139
FalI	AAGNNNNNCTT	4 cut(s)	584, 616, 1153, 1185
FaqI	GGGAC	1 cut(s)	864
FauI	CCCGC	3 cut(s)	103, 1671, 2214
FbaI	TGATCA	2 cut(s)	1198, 1909
FblI	GTMKAC	1 cut(s)	570
Fnu4HI	GCNGC	7 cut(s)	269, 392, 786, 1116, 1233, 1236, 2136
FokI	GGATG	6 cut(s)	497, 739, 1694, 1742, 2195, 2237
FriOI	GRGCYC	2 cut(s)	381, 1458
Fsp4HI	GCNGC	7 cut(s)	269, 392, 786, 1116, 1233, 1236, 2136
FspBI	CTAG	4 cut(s)	6, 1119, 1839, 2210
FspI	TGCGCA	1 cut(s)	2090
GlaI	GCGC	3 cut(s)	497, 1114, 2090
GluI	GCNGC	7 cut(s)	269, 392, 786, 1116, 1233, 1236, 2136
GsuI	CTGGAG	3 cut(s)	732, 1430, 2282
HaeII	RGCGCY	1 cut(s)	499
HaeIII	GGCC	5 cut(s)	118, 661, 761, 854, 1208
HapII	CCGG	4 cut(s)	119, 857, 961, 2231
HgaI	GACGC	1 cut(s)	920
HhaI	GCGC	3 cut(s)	498, 1115, 2091
Hin1I	GRCGYC	1 cut(s)	229
Hin6I	GCGC	3 cut(s)	496, 1113, 2089
HinP1I	GCGC	3 cut(s)	496, 1113, 2089
HincII	GTYRAC	1 cut(s)	571
HindII	GTYRAC	1 cut(s)	571
HindIII	AAGCTT	2 cut(s)	1178, 1548
HinfI	GANTC	9 cut(s)	837, 1060, 1469, 1487, 1526, 1733, 1918, 2069, 2107
HpaII	CCGG	4 cut(s)	119, 857, 961, 2231
HphI	GGTGA	3 cut(s)	85, 269, 2078
Hpy166II	GTNNAC	5 cut(s)	305, 307, 506, 571, 1955
Hpy188I	TCNGA	9 cut(s)	132, 234, 484, 655, 757, 1021, 1264, 1531, 2157
Hpy8I	GTNNAC	5 cut(s)	305, 307, 506, 571, 1955
Hpy99I	CGWCG	4 cut(s)	155, 158, 161, 575
HpyAV	CCTTC	2 cut(s)	899, 1771
HpyCH4IV	ACGT	4 cut(s)	229, 312, 1297, 1957
HpyF10VI	GCNNNNNNNGC	6 cut(s)	391, 445, 758, 826, 894, 1129
HpyF3I	CTNAG	7 cut(s)	500, 638, 846, 1071, 1109, 1596, 1989
HpySE526I	ACGT	4 cut(s)	229, 312, 1297, 1957
Hsp92I	GRCGYC	1 cut(s)	229
HspAI	GCGC	3 cut(s)	496, 1113, 2089
Kpn2I	TCCGGA	1 cut(s)	960
Ksp22I	TGATCA	2 cut(s)	1198, 1909
LmnI	GCTCC	4 cut(s)	376, 388, 551, 1411
Lsp1109I	GCAGC	7 cut(s)	255, 378, 772, 1127, 1219, 1222, 2122
LweI	GCATC	8 cut(s)	352, 761, 829, 1018, 1229, 1281, 1660, 1792
MaeI	CTAG	4 cut(s)	6, 1119, 1839, 2210
MaeII	ACGT	4 cut(s)	229, 312, 1297, 1957
MaeIII	GTNAC	4 cut(s)	43, 1100, 1293, 1741
MflI	RGATCY	4 cut(s)	790, 1691, 1841, 1900
MhlI	GDGCHC	3 cut(s)	381, 1216, 1458
MlsI	TGGCCA	1 cut(s)	1208
MluNI	TGGCCA	1 cut(s)	1208
MlyI	GAGTC	1 cut(s)	1742
MmeI	TCCRAC	2 cut(s)	720, 1132
Mox20I	TGGCCA	1 cut(s)	1208
MroI	TCCGGA	1 cut(s)	960
MroXI	GAANNNNTTC	1 cut(s)	1165
MscI	TGGCCA	1 cut(s)	1208
MseI	TTAA	9 cut(s)	195, 207, 219, 401, 431, 602, 1589, 1995, 2034
MslI	CAYNNNNRTG	4 cut(s)	542, 1323, 1976, 2064
Msp20I	TGGCCA	1 cut(s)	1208
MspCI	CTTAAG	1 cut(s)	601
MspI	CCGG	4 cut(s)	119, 857, 961, 2231
MspR9I	CCNGG	7 cut(s)	729, 795, 857, 1722, 2064, 2232, 2261
Mva1269I	GAATGC	1 cut(s)	283
MvaI	CCWGG	5 cut(s)	729, 795, 1722, 2064, 2261
MwoI	GCNNNNNNNGC	6 cut(s)	391, 445, 758, 826, 894, 1129
NciI	CCSGG	2 cut(s)	857, 2232
NcoI	CCATGG	1 cut(s)	2139
NlaIV	GGNNCC	3 cut(s)	744, 855, 1413
NmeAIII	GCCGAG	1 cut(s)	873
NmuCI	GTSAC	2 cut(s)	1100, 1293
NsbI	TGCGCA	1 cut(s)	2090
NspI	RCATGY	3 cut(s)	781, 833, 2271
NspV	TTCGAA	1 cut(s)	937
OliI	CACNNNNGTG	1 cut(s)	2064
PaeR7I	CTCGAG	2 cut(s)	252, 460
PagI	TCATGA	2 cut(s)	810, 840
PceI	AGGCCT	1 cut(s)	661
PciI	ACATGT	1 cut(s)	2267
PcsI	WCGNNNNNNNCGW	1 cut(s)	156
PctI	GAATGC	1 cut(s)	283
PdmI	GAANNNNTTC	1 cut(s)	1165
PfeI	GAWTC	8 cut(s)	837, 1060, 1469, 1487, 1526, 1918, 2069, 2107
PfoI	TCCNGGA	2 cut(s)	727, 793
PkrI	GCNGC	7 cut(s)	270, 393, 787, 1117, 1234, 1237, 2137
PleI	GAGTC	1 cut(s)	1741
PpsI	GAGTC	1 cut(s)	1741
PpuMI	RGGWCCY	2 cut(s)	689, 1478
PscI	ACATGT	1 cut(s)	2267
PshAI	GACNNNNGTC	1 cut(s)	126
Psp124BI	GAGCTC	1 cut(s)	381
Psp1406I	AACGTT	1 cut(s)	312
Psp5II	RGGWCCY	2 cut(s)	689, 1478
Psp6I	CCWGG	5 cut(s)	727, 793, 1720, 2062, 2259
PspGI	CCWGG	5 cut(s)	727, 793, 1720, 2062, 2259
PspN4I	GGNNCC	3 cut(s)	744, 855, 1413
PspPI	GGNCC	6 cut(s)	121, 689, 742, 760, 853, 1478
PspPPI	RGGWCCY	2 cut(s)	689, 1478
PstI	CTGCAG	1 cut(s)	790
PstNI	CAGNNNCTG	4 cut(s)	391, 649, 794, 1232
PsuI	RGATCY	4 cut(s)	790, 1691, 1841, 1900
RsaI	GTAC	2 cut(s)	306, 1284
RsaNI	GTAC	2 cut(s)	305, 1283
RseI	CAYNNNNRTG	4 cut(s)	542, 1323, 1976, 2064
SacI	GAGCTC	1 cut(s)	381
SalI	GTCGAC	1 cut(s)	569
SaqAI	TTAA	9 cut(s)	195, 207, 219, 401, 431, 602, 1589, 1995, 2034
SatI	GCNGC	7 cut(s)	269, 392, 786, 1116, 1233, 1236, 2136
Sau96I	GGNCC	6 cut(s)	121, 689, 742, 760, 853, 1478
SchI	GAGTC	1 cut(s)	1742
ScrFI	CCNGG	7 cut(s)	729, 795, 857, 1722, 2064, 2232, 2261
SduI	GDGCHC	3 cut(s)	381, 1216, 1458
SfaNI	GCATC	8 cut(s)	352, 761, 829, 1018, 1229, 1281, 1660, 1792
SfcI	CTRYAG	4 cut(s)	282, 345, 786, 1503
Sfr274I	CTCGAG	2 cut(s)	252, 460
SfuI	TTCGAA	1 cut(s)	937
SinI	GGWCC	4 cut(s)	121, 689, 742, 1478
SlaI	CTCGAG	2 cut(s)	252, 460
SmiMI	CAYNNNNRTG	4 cut(s)	542, 1323, 1976, 2064
SmlI	CTYRAG	7 cut(s)	252, 371, 460, 524, 601, 950, 1473
SmoI	CTYRAG	7 cut(s)	252, 371, 460, 524, 601, 950, 1473
SseBI	AGGCCT	1 cut(s)	661
SsiI	CCGC	4 cut(s)	110, 712, 1678, 2221
SspMI	CTAG	4 cut(s)	6, 1119, 1839, 2210
SstI	GAGCTC	1 cut(s)	381
StuI	AGGCCT	1 cut(s)	661
StyD4I	CCNGG	7 cut(s)	727, 793, 855, 1720, 2062, 2230, 2259
StyI	CCWWGG	3 cut(s)	1278, 1631, 2139
TaiI	ACGT	4 cut(s)	232, 315, 1300, 1960
TaqI	TCGA	8 cut(s)	86, 253, 461, 570, 937, 1808, 1890, 2072
TatI	WGTACW	1 cut(s)	304
TfiI	GAWTC	8 cut(s)	837, 1060, 1469, 1487, 1526, 1918, 2069, 2107
Tru1I	TTAA	9 cut(s)	195, 207, 219, 401, 431, 602, 1589, 1995, 2034
Tru9I	TTAA	9 cut(s)	195, 207, 219, 401, 431, 602, 1589, 1995, 2034
TscAI	CASTG	1 cut(s)	1216
TseFI	GTSAC	2 cut(s)	1100, 1293
TseI	GCWGC	7 cut(s)	268, 391, 785, 1115, 1232, 1235, 2135
Tsp45I	GTSAC	2 cut(s)	1100, 1293
TspGWI	ACGGA	1 cut(s)	1719
TspRI	CASTG	1 cut(s)	1216
Vha464I	CTTAAG	1 cut(s)	601
VpaK11BI	GGWCC	4 cut(s)	121, 689, 742, 1478
XapI	RAATTY	3 cut(s)	1141, 1768, 2144
XbaI	TCTAGA	1 cut(s)	1838
XceI	RCATGY	3 cut(s)	781, 833, 2271
XcmI	CCANNNNNNNNNTGG	2 cut(s)	544, 2257
XhoI	CTCGAG	2 cut(s)	252, 460
XmiI	GTMKAC	1 cut(s)	570
XmnI	GAANNNNTTC	1 cut(s)	1165
XspI	CTAG	4 cut(s)	6, 1119, 1839, 2210
ZraI	GACGTC	1 cut(s)	230

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.