Rh2AG526500

WLM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004067
Physical Location & Seq
Reverse (-)
75514155 .. 75519064
4910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG526500.1

Sequence Viewer

Length: 1416 bp
ATGATTACTTGTCTGCAGTTGAACCCTCCAGCATCCGAGGCCCTGAAAATAATGCACGTGCTTGCTGCAGATCCTGGAATTGTTGCTATCATGAACAAGCATCGCTGGCATGTGGGAATCATGACTGAGATGGCCCCGGTCGGTTATGTTGGCATAAGTCCCAAATGCATTCTCGGCTTCAACAAGAATAAGGGAGAGGAGATATCTCTGCGTCTTCGAACTGATGACCTTAAGGGTTTCCGGAAGTATGCAAGTATCAAGAAAACTCTTCTGCATGAGCTTGCTCACATGATATACTCTGAACATGATGCAACCTTTTATGCTTTTGATAAACAGTTGAATCAAGAAGCTGAGAGTTTAGATTGGACGAGATCAAGAGGTCACACACTAAGCGGAGCGAGGTATACAGAGCATTATGAAAATTCCTATGTTGGAGAGAGAGGTAATTCTTCTCACAAGCTTGGAGGAAATATGCCTGATCAACTGGCCAGTGCCCGAGCATCTTCAGTTGCTGCTGCTTATCATAGACTAGCAACTGCTTCTGATGATAGCATCTCCGAGGTACATGAACAGTCACATCTTGATGGTTTTGCGTTCCATGTGCATGAAAAAGACAAGCATAAGAACTTTTCAGGTAAAAGGGACTTGGAGATTGAAAGTTCTTACAAAGTTCAAAGGCAAAATGATCTGGAGCCTGATCCTGATGACTGTTTTGGCAATGAGAACAAGTTTGAGCCCAGTCTTGATGATTCTCAAGGAACTGATGAATCTTGCTCAAAATCTACAGGAAGCAGGAAAAATGTGGAACCTGATCCTGATAATCTCGAAGCTTGTTTGAAGTGTGATATCATAGCTGAACCTGAACCCTTACACCCTCAGGAGATGGAGATTTTGGAAAGCAGGTTTCAACCAAGGAATAATGTAGACGAACCTGATCCTGATGATTGTGATGTAAAGCGGGATGATTTGGGATCTAATTCAAATGGAAATATCACAAGACCTGGCAAGGATGAGTCTTTAGTTACTGAAACTACAAAATATGAAGCGAATTTGAGGAAGGCTTATGAAGAACCCGATCCTGATGCCTCTATGTCAAATGGCATTATTCAGCCAGTATCTAGATCTAATGATAATTTGGTGCTTCCAAATGAAATATCCAGTATGCAGATTGATGAACCAGATCCAGATGATCAAGAAATTCAAATAATTCAAGACCCTGTTTCAGTTGCTTGTAAACGTATGCAGAAGACCATTGAGATGTTGCAAGCTGAGGTTAATGCCACACAAGCTACCACAGTCCTCCAAACTCTATTTAAGATAATAAGGTACAATAACTTAATTTTGAGCATTGATTCATTCCTTGTCTTCATGAACACACAAAAACTATCAGGACTGTACATAACTGTAACTATATAA

Protein Analysis

471

Amino Acids

52.81

Weight (kDa)

4.96

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WLM PF08325 2 - 177 1.8e-47 WLM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 891
AccI GTMKAC 2 cut(s) 404, 924
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 2 cut(s) 393, 958
AclWI GGATC 7 cut(s) 65, 692, 806, 929, 979, 1070, 1175
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 3 cut(s) 421, 1048, 1197
AcuI CTGAAG 1 cut(s) 489
AcvI CACGTG 1 cut(s) 58
AfaI GTAC 3 cut(s) 564, 1328, 1397
AflII CTTAAG 1 cut(s) 230
AjnI CCWGG 2 cut(s) 73, 1000
AluBI AGCT 7 cut(s) 280, 350, 460, 830, 854, 1268, 1289
AluI AGCT 7 cut(s) 280, 350, 460, 830, 854, 1268, 1289
AlwI GGATC 7 cut(s) 65, 692, 806, 929, 979, 1070, 1175
AlwNI CAGNNNCTG 2 cut(s) 74, 512
Ama87I CYCGRG 1 cut(s) 495
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 3 cut(s) 39, 132, 486
ApeKI GCWGC 3 cut(s) 65, 512, 515
ApoI RAATTY 3 cut(s) 421, 1048, 1197
AspS9I GGNCC 2 cut(s) 40, 133
AsuC2I CCSGG 1 cut(s) 137
AsuII TTCGAA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 495
AxyI CCTNAGG 1 cut(s) 876
BaeGI GKGCMC 1 cut(s) 496
BalI TGGCCA 1 cut(s) 488
BanII GRGCYC 1 cut(s) 738
BbrPI CACGTG 1 cut(s) 58
BbsI GAAGAC 3 cut(s) 206, 1253, 1357
BbvCI CCTCAGC 1 cut(s) 1269
BbvI GCAGC 3 cut(s) 52, 499, 502
BccI CCATC 3 cut(s) 124, 578, 877
BcgI CGANNNNNNTGC 2 cut(s) 1064, 1098
BciT130I CCWGG 2 cut(s) 75, 1002
BclI TGATCA 2 cut(s) 478, 1189
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 2 cut(s) 530, 1119
BfmI CTRYAG 3 cut(s) 14, 66, 783
BfrI CTTAAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 891
BglII AGATCT 1 cut(s) 1121
BisI GCNGC 3 cut(s) 66, 513, 516
BlsI GCNGC 3 cut(s) 67, 514, 517
Bme1390I CCNGG 3 cut(s) 75, 137, 1002
BmeT110I CYCGRG 1 cut(s) 495
BmgT120I GGNCC 2 cut(s) 40, 133
BmiI GGNNCC 3 cut(s) 135, 693, 807
BmrFI CCNGG 3 cut(s) 75, 137, 1002
BmrI ACTGGG 1 cut(s) 732
BmsI GCATC 6 cut(s) 41, 109, 298, 509, 561, 1072
BmuI ACTGGG 1 cut(s) 732
BpiI GAAGAC 3 cut(s) 206, 1253, 1357
BpmI CTGGAG 2 cut(s) 12, 710
Bpu10I CCTNAGC 1 cut(s) 1269
Bpu14I TTCGAA 1 cut(s) 217
BpuEI CTTGAG 1 cut(s) 738
BpuMI CCSGG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 58
BsaJI CCNNGG 4 cut(s) 36, 135, 558, 911
BsaWI WCCGGW 1 cut(s) 240
Bse1I ACTGG 5 cut(s) 489, 489, 738, 1112, 1158
Bse21I CCTNAGG 1 cut(s) 876
Bse3DI GCAATG 1 cut(s) 724
BseAI TCCGGA 1 cut(s) 240
BseBI CCWGG 2 cut(s) 75, 1002
BseDI CCNNGG 4 cut(s) 36, 135, 558, 911
BseGI GGATG 3 cut(s) 32, 967, 1015
BseMI GCAATG 1 cut(s) 724
BseMII CTCAG 4 cut(s) 117, 342, 890, 1260
BseNI ACTGG 5 cut(s) 489, 489, 738, 1112, 1158
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 3 cut(s) 52, 499, 502
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 41, 134, 488
BsiEI CGRYCG 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 2 cut(s) 137, 241
BslFI GGGAC 2 cut(s) 144, 656
BsmFI GGGAC 2 cut(s) 144, 656
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 3 cut(s) 41, 134, 488
BsoBI CYCGRG 1 cut(s) 495
Bsp119I TTCGAA 1 cut(s) 217
Bsp1286I GDGCHC 2 cut(s) 496, 738
Bsp13I TCCGGA 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 1395
BspACI CCGC 2 cut(s) 393, 958
BspANI GGCC 3 cut(s) 41, 134, 488
BspCNI CTCAG 4 cut(s) 118, 343, 889, 1261
BspEI TCCGGA 1 cut(s) 240
BspHI TCATGA 3 cut(s) 90, 120, 1368
BspLI GGNNCC 3 cut(s) 135, 693, 807
BspMAI CTGCAG 2 cut(s) 18, 70
BspMI ACCTGC 1 cut(s) 891
BspPI GGATC 7 cut(s) 65, 692, 806, 929, 979, 1070, 1175
BspT104I TTCGAA 1 cut(s) 217
BspTI CTTAAG 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 724
BsrGI TGTACA 1 cut(s) 1395
BsrI ACTGG 5 cut(s) 489, 489, 738, 1112, 1158
BssECI CCNNGG 4 cut(s) 36, 135, 558, 911
BssNAI GTATAC 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 911
Bst1107I GTATAC 1 cut(s) 405
Bst2UI CCWGG 2 cut(s) 75, 1002
Bst4CI ACNGT 6 cut(s) 336, 573, 710, 1297, 1395, 1405
Bst6I CTCTTC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 230
BstAUI TGTACA 1 cut(s) 1395
BstBAI YACGTR 1 cut(s) 58
BstBI TTCGAA 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 63, 107, 282, 1266
BstDEI CTNAG 5 cut(s) 126, 351, 389, 876, 1269
BstF5I GGATG 3 cut(s) 32, 967, 1015
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 38, 106, 174, 1286
BstNI CCWGG 2 cut(s) 75, 1002
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 3 cut(s) 73, 135, 1000
BstSFI CTRYAG 3 cut(s) 14, 66, 783
BstSLI GKGCMC 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 52, 499, 502
BstV2I GAAGAC 3 cut(s) 206, 1253, 1357
BstX2I RGATCY 4 cut(s) 70, 971, 1121, 1180
BstYI RGATCY 4 cut(s) 70, 971, 1121, 1180
BstZ17I GTATAC 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 876
BsuRI GGCC 3 cut(s) 41, 134, 488
BtgZI GCGATG 1 cut(s) 86
BtsCI GGATG 3 cut(s) 32, 967, 1015
BtsIMutI CAGTG 1 cut(s) 496
BveI ACCTGC 1 cut(s) 891
Cac8I GCNNGC 4 cut(s) 63, 107, 282, 1266
CaiI CAGNNNCTG 2 cut(s) 74, 512
CciI TCATGA 3 cut(s) 90, 120, 1368
Cfr13I GGNCC 2 cut(s) 40, 133
CseI GACGC 1 cut(s) 200
Csp6I GTAC 3 cut(s) 563, 1327, 1396
CviQI GTAC 3 cut(s) 563, 1327, 1396
DdeI CTNAG 5 cut(s) 126, 351, 389, 876, 1269
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 1 cut(s) 738
Eco32I GATATC 2 cut(s) 204, 847
Eco57I CTGAAG 1 cut(s) 489
Eco72I CACGTG 1 cut(s) 58
Eco81I CCTNAGG 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 495
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 73, 1000
EcoRV GATATC 2 cut(s) 204, 847
EcoT14I CCWWGG 1 cut(s) 911
EcoT22I ATGCAT 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 911
FaqI GGGAC 2 cut(s) 144, 656
FauI CCCGC 1 cut(s) 951
FbaI TGATCA 2 cut(s) 478, 1189
FblI GTMKAC 2 cut(s) 404, 924
Fnu4HI GCNGC 3 cut(s) 66, 513, 516
FokI GGATG 3 cut(s) 19, 974, 1022
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 3 cut(s) 66, 513, 516
FspBI CTAG 2 cut(s) 530, 1119
GluI GCNGC 3 cut(s) 66, 513, 516
GsuI CTGGAG 2 cut(s) 12, 710
HaeIII GGCC 3 cut(s) 41, 134, 488
HapII CCGG 2 cut(s) 137, 241
HgaI GACGC 1 cut(s) 200
HindIII AAGCTT 2 cut(s) 458, 828
HinfI GANTC 6 cut(s) 117, 340, 749, 767, 1013, 1352
HpaII CCGG 2 cut(s) 137, 241
Hpy166II GTNNAC 3 cut(s) 405, 925, 1235
Hpy188I TCNGA 4 cut(s) 37, 301, 544, 559
Hpy8I GTNNAC 3 cut(s) 405, 925, 1235
HpyAV CCTTC 1 cut(s) 1051
HpyCH4III ACNGT 6 cut(s) 336, 573, 710, 1297, 1395, 1405
HpyCH4IV ACGT 2 cut(s) 57, 1237
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 106, 174, 1286
HpyF3I CTNAG 5 cut(s) 126, 351, 389, 876, 1269
HpySE526I ACGT 2 cut(s) 57, 1237
Kpn2I TCCGGA 1 cut(s) 240
Ksp22I TGATCA 2 cut(s) 478, 1189
LmnI GCTCC 2 cut(s) 395, 691
Lsp1109I GCAGC 3 cut(s) 52, 499, 502
LweI GCATC 6 cut(s) 41, 109, 298, 509, 561, 1072
MaeI CTAG 2 cut(s) 530, 1119
MaeII ACGT 2 cut(s) 57, 1237
MaeIII GTNAC 4 cut(s) 380, 573, 1021, 1405
MboII GAAGA 7 cut(s) 206, 260, 441, 495, 1079, 1258, 1357
MflI RGATCY 4 cut(s) 70, 971, 1121, 1180
MhlI GDGCHC 2 cut(s) 496, 738
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 9 cut(s) 78, 421, 445, 976, 1048, 1132, 1197, 1206, 1338
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 1022
MmeI TCCRAC 1 cut(s) 412
Mox20I TGGCCA 1 cut(s) 488
Mph1103I ATGCAT 1 cut(s) 170
MroI TCCGGA 1 cut(s) 240
MscI TGGCCA 1 cut(s) 488
MseI TTAA 4 cut(s) 231, 1275, 1314, 1337
MslI CAYNNNNRTG 3 cut(s) 582, 603, 1256
Msp20I TGGCCA 1 cut(s) 488
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 2 cut(s) 137, 241
MspR9I CCNGG 3 cut(s) 75, 137, 1002
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 2 cut(s) 75, 1002
MwoI GCNNNNNNNGC 4 cut(s) 38, 106, 174, 1286
NciI CCSGG 1 cut(s) 137
NlaIV GGNNCC 3 cut(s) 135, 693, 807
NmeAIII GCCGAG 1 cut(s) 153
NmuCI GTSAC 2 cut(s) 380, 573
NsiI ATGCAT 1 cut(s) 170
NspI RCATGY 1 cut(s) 113
NspV TTCGAA 1 cut(s) 217
PagI TCATGA 3 cut(s) 90, 120, 1368
PctI GAATGC 1 cut(s) 168
PfeI GAWTC 5 cut(s) 117, 340, 749, 767, 1352
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 3 cut(s) 67, 514, 517
PleI GAGTC 1 cut(s) 1021
PmaCI CACGTG 1 cut(s) 58
PmlI CACGTG 1 cut(s) 58
PpsI GAGTC 1 cut(s) 1021
Ppu21I YACGTR 1 cut(s) 58
Psp6I CCWGG 2 cut(s) 73, 1000
PspCI CACGTG 1 cut(s) 58
PspGI CCWGG 2 cut(s) 73, 1000
PspN4I GGNNCC 3 cut(s) 135, 693, 807
PspPI GGNCC 2 cut(s) 40, 133
PstI CTGCAG 2 cut(s) 18, 70
PstNI CAGNNNCTG 2 cut(s) 74, 512
PsuI RGATCY 4 cut(s) 70, 971, 1121, 1180
RsaI GTAC 3 cut(s) 564, 1328, 1397
RsaNI GTAC 3 cut(s) 563, 1327, 1396
RseI CAYNNNNRTG 3 cut(s) 582, 603, 1256
SaqAI TTAA 4 cut(s) 231, 1275, 1314, 1337
SatI GCNGC 3 cut(s) 66, 513, 516
Sau96I GGNCC 2 cut(s) 40, 133
SchI GAGTC 1 cut(s) 1022
ScrFI CCNGG 3 cut(s) 75, 137, 1002
SduI GDGCHC 2 cut(s) 496, 738
SfaNI GCATC 6 cut(s) 41, 109, 298, 509, 561, 1072
SfcI CTRYAG 3 cut(s) 14, 66, 783
SfuI TTCGAA 1 cut(s) 217
SmiMI CAYNNNNRTG 3 cut(s) 582, 603, 1256
SmlI CTYRAG 2 cut(s) 230, 753
SmoI CTYRAG 2 cut(s) 230, 753
Sse9I AATT 9 cut(s) 78, 421, 445, 976, 1048, 1132, 1197, 1206, 1338
SsiI CCGC 2 cut(s) 393, 958
SspMI CTAG 2 cut(s) 530, 1119
StyD4I CCNGG 3 cut(s) 73, 135, 1000
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 6 cut(s) 336, 573, 710, 1297, 1395, 1405
TaiI ACGT 2 cut(s) 60, 1240
TaqI TCGA 2 cut(s) 217, 825
TasI AATT 9 cut(s) 78, 421, 445, 976, 1048, 1132, 1197, 1206, 1338
TatI WGTACW 1 cut(s) 1395
TfiI GAWTC 5 cut(s) 117, 340, 749, 767, 1352
Tru1I TTAA 4 cut(s) 231, 1275, 1314, 1337
Tru9I TTAA 4 cut(s) 231, 1275, 1314, 1337
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 2 cut(s) 380, 573
TseI GCWGC 3 cut(s) 65, 512, 515
Tsp45I GTSAC 2 cut(s) 380, 573
TspRI CASTG 1 cut(s) 496
Vha464I CTTAAG 1 cut(s) 230
XapI RAATTY 3 cut(s) 421, 1048, 1197
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 404, 924
XspI CTAG 2 cut(s) 530, 1119
Zsp2I ATGCAT 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.