pycom09g05370

Rrp15p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0009316
Physical Location & Seq
Forward (+)
3995346 .. 3997816
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ATGGCCGAAGAAAACCATCTTCCAGAAGAAGCAGCAGAGCCGAGGAAGCGGAAAATAGGGAAGAAGAAGGGCTCCAAGGCAAAGAAGAGGGCGAAGATGTTACATTCGTCGGAGGAAATCAGAGCCATGAAGCCGAAGAAGATTAACAAGAAAATGAAGAAACTGTTTCTTAAACGAGCCCGGGAGTACGATTCGGAGGAGGAGGAGGAGTCCAAACAGGAACATGTGAAACCGAAACCGCATCCGCAACCGTTGGGAAACAATGAGGAGGAGCCGAGTGGCGGTGGTGGGTCGTCTTCAGGGGAAGAGGCGGAGGAGGGTGTTGGTGGCCGCAATAGGGATGATGGCTTTTCGGATGACGAGGAGCACGGCGAGATTCTGCCCGGAATCATGAAGTTTTCGGAGGGTTGCAAGGCGTTTAGGTTGGCATTCAGGAGTATAATCAAGAAGTCTGTTTCTCAGGATGCAGTGGGACCCGTGATGTCGGCGCACAAGAAGCTTGTTGCAGAAAAGCTTGCCGAGGAGCAGACCGAGCGCAAGGCAAAGGGCGAGGCTAAGAAGGAAAAGCACACGGTGATAGAAAAGGGGCATGTGATGCCGGCCAACTATTTAGACACACACGAAAAGTTTCTAATCGGTGTTGCTACTAAAGGAGTGGTGAAGTTGTTCAATGCTGTCAACAAGGCACAAAATGCTCAGAAAGGATTGAATCCTTCCAAGTTTAAAGATGCCAAAGTGATAAAAAAGCGGAGGAAAGAAGCATTCTTTTCCGAGTTGGGCAAAACATCTTTGAAGGCTCACACGTCTAAAGGCGCAGTGGATGGTGAAGGGCCTGCCTGGGCACCATTAAGAGATAATTACATGCTCACAAATCCCAAATTGAAAGACTGGGATAAGATGCCGACCGTTGCGGAAGATGACATTGGAAGGGTGGCTGGAGATAGTTCCGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rrp15p

Protein Analysis

320

Amino Acids

35.41

Weight (kDa)

9.27

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rrp15p PF07890 140 - 259 4.5e-21 Rrp15p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 841
AciI CCGC 8 cut(s) 49, 239, 245, 282, 311, 331, 748, 911
AcoI YGGCCR 3 cut(s) 3, 328, 600
AcuI CTGAAG 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 281, 337
AflIII ACRYGT 2 cut(s) 223, 801
AgsI TTSAA 4 cut(s) 670, 709, 793, 883
AjiI CACGTC 1 cut(s) 804
AjnI CCWGG 1 cut(s) 836
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
AluBI AGCT 2 cut(s) 499, 514
AluI AGCT 2 cut(s) 499, 514
Alw21I GWGCWC 1 cut(s) 369
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 4 cut(s) 3, 328, 600, 830
ApeKI GCWGC 1 cut(s) 32
Asp700I GAANNNNTTC 2 cut(s) 627, 665
AspLEI GCGC 3 cut(s) 490, 537, 815
AspS9I GGNCC 2 cut(s) 473, 830
AsuC2I CCSGG 3 cut(s) 181, 182, 384
AsuHPI GGTGA 3 cut(s) 586, 670, 836
AvaI CYCGRG 1 cut(s) 180
AvaII GGWCC 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 844
BanI GGYRCC 1 cut(s) 841
BanII GRGCYC 2 cut(s) 74, 181
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 44
BccI CCATC 3 cut(s) 24, 338, 815
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 838
BcnI CCSGG 3 cut(s) 181, 182, 384
BisI GCNGC 2 cut(s) 33, 331
BlsI GCNGC 2 cut(s) 34, 332
Bme1390I CCNGG 4 cut(s) 181, 182, 384, 838
Bme18I GGWCC 1 cut(s) 473
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 804
BmgT120I GGNCC 2 cut(s) 473, 830
BmiI GGNNCC 5 cut(s) 73, 273, 474, 475, 843
BmrFI CCNGG 4 cut(s) 181, 182, 384, 838
BmrI ACTGGG 1 cut(s) 898
BmsI GCATC 5 cut(s) 250, 454, 585, 718, 888
BmuI ACTGGG 1 cut(s) 898
BpiI GAAGAC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 957
BpuMI CCSGG 3 cut(s) 181, 182, 384
BsaJI CCNNGG 5 cut(s) 41, 75, 180, 519, 837
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 337
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 1 cut(s) 893
BseBI CCWGG 1 cut(s) 838
BseDI CCNNGG 5 cut(s) 41, 75, 180, 519, 837
BseGI GGATG 5 cut(s) 241, 346, 361, 469, 826
BseLI CCNNNNNNNGG 2 cut(s) 281, 337
BseMII CTCAG 2 cut(s) 473, 710
BseNI ACTGG 1 cut(s) 893
BseRI GAGGAG 9 cut(s) 212, 215, 218, 221, 281, 284, 329, 377, 536
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 1 cut(s) 44
Bsh1285I CGRYCG 1 cut(s) 906
BshFI GGCC 4 cut(s) 5, 330, 602, 832
BshNI GGYRCC 1 cut(s) 841
BsiEI CGRYCG 1 cut(s) 906
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 181, 384, 599
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 2 cut(s) 281, 337
BsmFI GGGAC 1 cut(s) 486
BsmI GAATGC 2 cut(s) 428, 761
BsnI GGCC 4 cut(s) 5, 330, 602, 832
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 4 cut(s) 74, 181, 369, 844
BspACI CCGC 8 cut(s) 49, 239, 245, 282, 311, 331, 748, 911
BspANI GGCC 4 cut(s) 5, 330, 602, 832
BspCNI CTCAG 2 cut(s) 472, 709
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 5 cut(s) 73, 273, 474, 475, 843
BspT107I GGYRCC 1 cut(s) 841
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 1 cut(s) 893
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 5 cut(s) 41, 75, 180, 519, 837
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 838
Bst4CI ACNGT 4 cut(s) 165, 252, 574, 907
Bst6I CTCTTC 2 cut(s) 80, 300
BstAPI GCANNNNNTGC 2 cut(s) 595, 692
BstC8I GCNNGC 3 cut(s) 516, 600, 834
BstDEI CTNAG 3 cut(s) 459, 555, 696
BstF5I GGATG 5 cut(s) 241, 346, 361, 469, 826
BstHHI GCGC 3 cut(s) 490, 537, 815
BstMCI CGRYCG 1 cut(s) 906
BstMWI GCNNNNNNNGC 5 cut(s) 46, 496, 532, 595, 692
BstNI CCWGG 1 cut(s) 838
BstNSI RCATGY 3 cut(s) 227, 593, 865
BstSCI CCNGG 4 cut(s) 179, 180, 382, 836
BstSLI GKGCMC 1 cut(s) 844
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 288
BsuRI GGCC 4 cut(s) 5, 330, 602, 832
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 5 cut(s) 241, 346, 361, 469, 826
BtsI GCAGTG 2 cut(s) 474, 822
BtsIMutI CAGTG 2 cut(s) 474, 822
Cac8I GCNNGC 3 cut(s) 516, 600, 834
CciI TCATGA 1 cut(s) 390
CfoI GCGC 3 cut(s) 490, 537, 815
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 473, 830
Cfr9I CCCGGG 1 cut(s) 180
Csp6I GTAC 1 cut(s) 187
CviAII CATG 5 cut(s) 127, 224, 391, 590, 862
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 3 cut(s) 459, 555, 696
DraI TTTAAA 1 cut(s) 724
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 3 cut(s) 3, 328, 600
Eam1104I CTCTTC 2 cut(s) 80, 300
EarI CTCTTC 2 cut(s) 80, 300
EciI GGCGGA 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 2 cut(s) 74, 181
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 180
EcoO109I RGGNCCY 2 cut(s) 473, 830
EcoRII CCWGG 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 2 cut(s) 74, 181
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 5 cut(s) 130, 227, 394, 593, 865
FaiI YATR 6 cut(s) 128, 225, 392, 440, 591, 863
FaqI GGGAC 1 cut(s) 486
FatI CATG 5 cut(s) 126, 223, 390, 589, 861
Fnu4HI GCNGC 2 cut(s) 33, 331
FokI GGATG 5 cut(s) 228, 353, 368, 476, 833
FriOI GRGCYC 2 cut(s) 74, 181
Fsp4HI GCNGC 2 cut(s) 33, 331
GlaI GCGC 3 cut(s) 489, 536, 814
GluI GCNGC 2 cut(s) 33, 331
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 4 cut(s) 5, 330, 602, 832
HapII CCGG 3 cut(s) 181, 384, 599
HhaI GCGC 3 cut(s) 490, 537, 815
Hin1II CATG 5 cut(s) 130, 227, 394, 593, 865
Hin6I GCGC 3 cut(s) 488, 535, 813
HinP1I GCGC 3 cut(s) 488, 535, 813
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HindIII AAGCTT 2 cut(s) 497, 512
HinfI GANTC 5 cut(s) 191, 209, 376, 387, 709
HpaII CCGG 3 cut(s) 181, 384, 599
HphI GGTGA 3 cut(s) 586, 670, 836
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 8 cut(s) 112, 122, 196, 355, 403, 699, 772, 949
Hpy188III TCNNGA 5 cut(s) 23, 391, 433, 445, 461
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 6 cut(s) 61, 553, 723, 787, 821, 921
HpyCH4III ACNGT 4 cut(s) 165, 252, 574, 907
HpyCH4IV ACGT 1 cut(s) 803
HpyCH4V TGCA 3 cut(s) 411, 467, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 496, 532, 595, 692
HpyF3I CTNAG 3 cut(s) 459, 555, 696
HpySE526I ACGT 1 cut(s) 803
Hsp92II CATG 5 cut(s) 130, 227, 394, 593, 865
HspAI GCGC 3 cut(s) 488, 535, 813
KflI GGGWCCC 1 cut(s) 473
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
LmnI GCTCC 4 cut(s) 77, 271, 364, 523
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 5 cut(s) 250, 454, 585, 718, 888
MaeII ACGT 1 cut(s) 803
MaeIII GTNAC 1 cut(s) 99
MhlI GDGCHC 4 cut(s) 74, 181, 369, 844
MluCI AATT 2 cut(s) 856, 878
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 90
MroNI GCCGGC 1 cut(s) 598
MroXI GAANNNNTTC 2 cut(s) 627, 665
MseI TTAA 4 cut(s) 144, 171, 723, 848
MspI CCGG 3 cut(s) 181, 384, 599
MspR9I CCNGG 4 cut(s) 181, 182, 384, 838
Mva1269I GAATGC 2 cut(s) 428, 761
MvaI CCWGG 1 cut(s) 838
MwoI GCNNNNNNNGC 5 cut(s) 46, 496, 532, 595, 692
NaeI GCCGGC 1 cut(s) 600
NciI CCSGG 3 cut(s) 181, 182, 384
NgoMIV GCCGGC 1 cut(s) 598
NlaIII CATG 5 cut(s) 130, 227, 394, 593, 865
NlaIV GGNNCC 5 cut(s) 73, 273, 474, 475, 843
NmeAIII GCCGAG 3 cut(s) 66, 300, 544
NspI RCATGY 3 cut(s) 227, 593, 865
PagI TCATGA 1 cut(s) 390
PciI ACATGT 1 cut(s) 223
PctI GAATGC 2 cut(s) 428, 761
PdiI GCCGGC 1 cut(s) 600
PdmI GAANNNNTTC 2 cut(s) 627, 665
PfeI GAWTC 4 cut(s) 191, 376, 387, 709
PkrI GCNGC 2 cut(s) 34, 332
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 473
PscI ACATGT 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 836
PspGI CCWGG 1 cut(s) 836
PspN4I GGNNCC 5 cut(s) 73, 273, 474, 475, 843
PspPI GGNCC 2 cut(s) 473, 830
PspPPI RGGWCCY 1 cut(s) 473
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 4 cut(s) 144, 171, 723, 848
SatI GCNGC 2 cut(s) 33, 331
Sau96I GGNCC 2 cut(s) 473, 830
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 181, 182, 384, 838
SduI GDGCHC 4 cut(s) 74, 181, 369, 844
SetI ASST 4 cut(s) 425, 501, 516, 806
SfaNI GCATC 5 cut(s) 250, 454, 585, 718, 888
SinI GGWCC 1 cut(s) 473
SmaI CCCGGG 1 cut(s) 182
Sse9I AATT 2 cut(s) 856, 878
SsiI CCGC 8 cut(s) 49, 239, 245, 282, 311, 331, 748, 911
StyD4I CCNGG 4 cut(s) 179, 180, 382, 836
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 4 cut(s) 165, 252, 574, 907
TaiI ACGT 1 cut(s) 806
TaqII GACCGA 1 cut(s) 545
TasI AATT 2 cut(s) 856, 878
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 4 cut(s) 191, 376, 387, 709
Tru1I TTAA 4 cut(s) 144, 171, 723, 848
Tru9I TTAA 4 cut(s) 144, 171, 723, 848
TscAI CASTG 2 cut(s) 474, 822
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 3 cut(s) 143, 170, 407
TspMI CCCGGG 1 cut(s) 180
TspRI CASTG 2 cut(s) 474, 822
VpaK11BI GGWCC 1 cut(s) 473
XceI RCATGY 3 cut(s) 227, 593, 865
XmaI CCCGGG 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 627, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.