pycom09g11000

WD-40 repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003346
Physical Location & Seq
Forward (+)
9154614 .. 9160264
5651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1305 bp
ATGAAAGGGAAAGCTACGGAGGCGTCGGTGAACGATCGATACACGCAGTGGAAGTCGCTGGTGCCAGTGCTCTACGACTGGCTCGCCAATCACAACCTTGTTTGGCCTTCTCTCTCTTGCAGATGGGGACCGCAGCTGGAGCAAGCCACTTACAAGAGTCGTCAGCGCCTTTTTCTTTCGGAACAGACTGATGGCAGTGTTCCAAATACACTTGTCATTGCAAACTGTGAAGTTGTCAAACCCAGGGTTGCAGCTGCCGAGCACATATCACAGGTGAACAGAATCAGGGAACTCCCTCAGAATAGTAAGATAGTGGCAACACACACTGACAGTCCTGATGTTCTCATTTGGGATATTGAGGCTCAACCTAATCGCCATGCTGTTCTGGGAGCTTCAGATTCTCGTCCAGATCTGCTATTGATAGGGCATCGAGATAATGCTGAATTTGCTCTTGCCATGTGCCCATCTGAACCCTTTGTGCTCTCTGGAGGGAAGGATAAATCAGTGATTTTGTGGAGTATTCATGATCATATCTCATCTTTAGCATCAGAACCAGGATCTGGAGGTGGCAGTGCTAAACATTCTTCTAAGATTGGTGGGAGTAACGATAAACTCAGAGACAGCCCTTCTGTTGAGCCACGGGGTGTCTATAAAGGGCATGAGGATACTGTTGAGGATGTGCAGTTCTGCCCATCTAGTGCACCGGAGTTCTGTAGTGTAGGTGATGATTCTTGCCTCATACTATGGGATACAAGAGCTGGATCTAGTCCAGCTGTCAAGGTTGACAAAGCTCATGATGCTGACCTTCATTGCGTTGATTGGAATCCACATGACGTCAATCTCATACTTACAGGGTCTGCCGATAATACTATTCGCTTGTTTGACCGCCGATATCTCACTTCTGGAGGAGTTGGATCACCTGTTCATACATTTGAGGGCCACAATGCAGCTGTCCTCTGCGTACAGTGGTCTCCTCACAAGGCATCTGTTTTTGGAAGCTCTGCCGAGGATGGCTTACTAAACATCTGGGATCATGAAAAGGTTGGCAAGAATGGATCAAAGCCGTCAAATGCTCCTCCAGGATTGTTCTTTCGACATGCTGGTCATAGGGACAAGGTTGTTGACTTTCATTGGAATGCGTCTGATCCGTGGACGATAGCTAGTGTCTCTGATGATTGCGGAAGTACCGGAGGTGGTGGTACCCTCCAGATATGGCGGATGATTGATTTGATTTACAGGCCCGAGGAAGAGGTCCTCGCAGAGCTGGATACGTTCAAATCTCACCTAACAACATGCCAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

435

Amino Acids

47.43

Weight (kDa)

5.7

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 12 - 79 1.3e-19 Histone-binding protein RBBP4 or subunit C of CAF1 complex
Beta-prop_TEP1_2nd PF25047 210 - 348 7.4e-09 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 211 - 295 1.1e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 215 - 347 1e-18 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 218 - 294 3.5e-09 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 219 - 346 2.8e-13 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 233 - 351 9.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 239 - 392 1e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 263 - 391 1.5e-10 Prp19 WD40 domain
Beta-prop_EIPR1 PF23609 263 - 345 1.8e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 265 - 347 2.1e-11 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 267 - 347 8.2e-07 WDHD1 first WD40 domain
WD40 PF00400 305 - 344 2.8e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1101
AatII GACGTC 1 cut(s) 837
Acc65I GGTACC 1 cut(s) 1199
AccB1I GGYRCC 2 cut(s) 61, 1199
AccB7I CCANNNNNTGG 1 cut(s) 560
AciI CCGC 4 cut(s) 131, 886, 1179, 1216
AclWI GGATC 6 cut(s) 565, 769, 922, 1038, 1063, 1139
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 2 cut(s) 23, 834
AdeI CACNNNGTG 2 cut(s) 48, 644
AfaI GTAC 3 cut(s) 963, 1186, 1201
AfiI CCNNNNNNNGG 1 cut(s) 560
AgsI TTSAA 1 cut(s) 1276
AjnI CCWGG 4 cut(s) 242, 553, 1078, 1296
AloI GAACNNNNNNTCC 4 cut(s) 668, 700, 906, 938
Alw21I GWGCWC 4 cut(s) 72, 264, 483, 703
Alw26I GTCTC 3 cut(s) 612, 975, 1171
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 6 cut(s) 565, 769, 922, 1038, 1063, 1139
AlwNI CAGNNNCTG 2 cut(s) 560, 857
Ama87I CYCGRG 1 cut(s) 1241
AoxI GGCC 3 cut(s) 104, 937, 1238
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 4 cut(s) 133, 251, 254, 947
ApoI RAATTY 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 1199
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 4 cut(s) 128, 937, 1239, 1252
AsuHPI GGTGA 5 cut(s) 40, 286, 734, 909, 1274
AvaI CYCGRG 1 cut(s) 1241
AvaII GGWCC 2 cut(s) 128, 1252
BaeGI GKGCMC 2 cut(s) 464, 703
BanI GGYRCC 2 cut(s) 61, 1199
Bbv12I GWGCWC 4 cut(s) 72, 264, 483, 703
BbvI GCAGC 4 cut(s) 145, 241, 263, 959
BccI CCATC 5 cut(s) 117, 185, 472, 700, 1004
BceAI ACGGC 1 cut(s) 1048
BciT130I CCWGG 4 cut(s) 244, 555, 1080, 1298
BciVI GTATCC 3 cut(s) 658, 742, 1261
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 3 cut(s) 612, 975, 1171
BfaI CTAG 3 cut(s) 696, 765, 1161
BfmI CTRYAG 1 cut(s) 712
BfoI RGCGCY 1 cut(s) 169
BfuI GTATCC 3 cut(s) 658, 742, 1261
BglII AGATCT 1 cut(s) 409
BisI GCNGC 4 cut(s) 134, 252, 255, 948
BlsI GCNGC 4 cut(s) 135, 253, 256, 949
Bme1390I CCNGG 4 cut(s) 244, 555, 1080, 1298
Bme18I GGWCC 2 cut(s) 128, 1252
BmeT110I CYCGRG 1 cut(s) 1241
BmgT120I GGNCC 4 cut(s) 128, 937, 1239, 1252
BmiI GGNNCC 3 cut(s) 63, 129, 1201
BmrFI CCNGG 4 cut(s) 244, 555, 1080, 1298
BmsI GCATC 4 cut(s) 436, 554, 787, 992
BpmI CTGGAG 6 cut(s) 158, 507, 582, 924, 1062, 1190
Bsa29I ATCGAT 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 822
BsaHI GRCGYC 2 cut(s) 23, 834
BsaI GGTCTC 1 cut(s) 975
BsaJI CCNNGG 6 cut(s) 242, 243, 638, 1005, 1148, 1242
BsaWI WCCGGW 2 cut(s) 703, 1187
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 2 cut(s) 65, 83
Bse3DI GCAATG 2 cut(s) 216, 808
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 4 cut(s) 244, 555, 1080, 1298
BseCI ATCGAT 1 cut(s) 37
BseDI CCNNGG 6 cut(s) 242, 243, 638, 1005, 1148, 1242
BseGI GGATG 3 cut(s) 682, 1015, 1224
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMI GCAATG 2 cut(s) 216, 808
BseMII CTCAG 2 cut(s) 311, 628
BseNI ACTGG 2 cut(s) 65, 83
BseRI GAGGAG 3 cut(s) 921, 963, 1065
BseSI GKGCMC 2 cut(s) 464, 703
BseXI GCAGC 4 cut(s) 145, 241, 263, 959
BsgI GTGCAG 1 cut(s) 701
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 106, 939, 1240
BshNI GGYRCC 2 cut(s) 61, 1199
BshVI ATCGAT 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 4 cut(s) 72, 264, 483, 703
BsiHKCI CYCGRG 1 cut(s) 1241
BsiSI CCGG 2 cut(s) 704, 1188
BslFI GGGAC 2 cut(s) 141, 1124
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 3 cut(s) 612, 975, 1171
BsmFI GGGAC 2 cut(s) 141, 1124
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 3 cut(s) 106, 939, 1240
Bso31I GGTCTC 1 cut(s) 975
BsoBI CYCGRG 1 cut(s) 1241
Bsp1286I GDGCHC 5 cut(s) 72, 264, 464, 483, 703
Bsp143I GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
BspACI CCGC 4 cut(s) 131, 886, 1179, 1216
BspANI GGCC 3 cut(s) 106, 939, 1240
BspCNI CTCAG 2 cut(s) 310, 627
BspDI ATCGAT 1 cut(s) 37
BspHI TCATGA 3 cut(s) 523, 793, 1033
BspLI GGNNCC 3 cut(s) 63, 129, 1201
BspPI GGATC 6 cut(s) 565, 769, 922, 1038, 1063, 1139
BspT107I GGYRCC 2 cut(s) 61, 1199
BspTNI GGTCTC 1 cut(s) 975
BsrDI GCAATG 2 cut(s) 216, 808
BsrI ACTGG 2 cut(s) 65, 83
BssECI CCNNGG 6 cut(s) 242, 243, 638, 1005, 1148, 1242
BssMI GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
BssNI GRCGYC 2 cut(s) 23, 834
Bst2UI CCWGG 4 cut(s) 244, 555, 1080, 1298
Bst4CI ACNGT 4 cut(s) 227, 332, 670, 966
Bst6I CTCTTC 1 cut(s) 1242
BstACI GRCGYC 2 cut(s) 23, 834
BstC8I GCNNGC 2 cut(s) 84, 144
BstDEI CTNAG 3 cut(s) 297, 588, 614
BstDSI CCRYGG 2 cut(s) 638, 1148
BstF5I GGATG 3 cut(s) 682, 1015, 1224
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 9 cut(s) 37, 412, 529, 560, 764, 917, 1033, 1058, 1147
BstMAI GTCTC 3 cut(s) 612, 975, 1171
BstMBI GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 4 cut(s) 20, 139, 446, 797
BstNI CCWGG 4 cut(s) 244, 555, 1080, 1298
BstNSI RCATGY 2 cut(s) 1100, 1296
BstSCI CCNGG 4 cut(s) 242, 553, 1078, 1296
BstSFI CTRYAG 1 cut(s) 712
BstSLI GKGCMC 2 cut(s) 464, 703
BstV1I GCAGC 4 cut(s) 145, 241, 263, 959
BstX2I RGATCY 3 cut(s) 409, 557, 761
BstYI RGATCY 3 cut(s) 409, 557, 761
Bsu15I ATCGAT 1 cut(s) 37
BsuI GTATCC 3 cut(s) 658, 742, 1261
BsuRI GGCC 3 cut(s) 106, 939, 1240
BsuTUI ATCGAT 1 cut(s) 37
BtgI CCRYGG 2 cut(s) 638, 1148
BtsCI GGATG 3 cut(s) 682, 1015, 1224
BtsI GCAGTG 3 cut(s) 53, 202, 577
BtsIMutI CAGTG 7 cut(s) 53, 72, 202, 324, 510, 577, 971
Cac8I GCNNGC 2 cut(s) 84, 144
CaiI CAGNNNCTG 2 cut(s) 560, 857
CciI TCATGA 3 cut(s) 523, 793, 1033
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 4 cut(s) 128, 937, 1239, 1252
ClaI ATCGAT 1 cut(s) 37
CseI GACGC 2 cut(s) 12, 1128
Csp6I GTAC 3 cut(s) 962, 1185, 1200
CviAII CATG 9 cut(s) 377, 457, 524, 659, 794, 830, 1034, 1097, 1293
CviQI GTAC 3 cut(s) 962, 1185, 1200
DdeI CTNAG 3 cut(s) 297, 588, 614
DpnI GATC 9 cut(s) 36, 411, 528, 559, 763, 916, 1032, 1057, 1146
DpnII GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
DraIII CACNNNGTG 2 cut(s) 48, 644
DrdI GACNNNNNNGTC 1 cut(s) 1101
DseDI GACNNNNNNGTC 1 cut(s) 1101
Eam1104I CTCTTC 1 cut(s) 1242
EarI CTCTTC 1 cut(s) 1242
EciI GGCGGA 1 cut(s) 1231
Eco31I GGTCTC 1 cut(s) 975
Eco32I GATATC 1 cut(s) 893
Eco47I GGWCC 2 cut(s) 128, 1252
Eco57I CTGAAG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 1241
EcoO109I RGGNCCY 1 cut(s) 1252
EcoRII CCWGG 4 cut(s) 242, 553, 1078, 1296
EcoRV GATATC 1 cut(s) 893
FaeI CATG 9 cut(s) 380, 460, 527, 662, 797, 833, 1037, 1100, 1296
FaqI GGGAC 2 cut(s) 141, 1124
FatI CATG 9 cut(s) 376, 456, 523, 658, 793, 829, 1033, 1096, 1292
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 4 cut(s) 134, 252, 255, 948
FokI GGATG 3 cut(s) 689, 1022, 1231
Fsp4HI GCNGC 4 cut(s) 134, 252, 255, 948
FspBI CTAG 3 cut(s) 696, 765, 1161
GlaI GCGC 1 cut(s) 167
GluI GCNGC 4 cut(s) 134, 252, 255, 948
GsuI CTGGAG 6 cut(s) 158, 507, 582, 924, 1062, 1190
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 3 cut(s) 106, 939, 1240
HapII CCGG 2 cut(s) 704, 1188
HgaI GACGC 2 cut(s) 12, 1128
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 2 cut(s) 23, 834
Hin1II CATG 9 cut(s) 380, 460, 527, 662, 797, 833, 1037, 1100, 1296
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 2 cut(s) 784, 1123
HindII GTYRAC 2 cut(s) 784, 1123
HinfI GANTC 5 cut(s) 157, 282, 398, 728, 823
HpaII CCGG 2 cut(s) 704, 1188
HphI GGTGA 5 cut(s) 40, 286, 734, 909, 1274
Hpy166II GTNNAC 6 cut(s) 31, 277, 701, 784, 1123, 1152
Hpy188I TCNGA 8 cut(s) 181, 300, 397, 469, 550, 617, 1144, 1171
Hpy8I GTNNAC 6 cut(s) 31, 277, 701, 784, 1123, 1152
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 117, 487, 636, 815
HpyCH4III ACNGT 4 cut(s) 227, 332, 670, 966
HpyCH4IV ACGT 2 cut(s) 834, 1271
HpyCH4V TGCA 6 cut(s) 120, 221, 251, 682, 701, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 139, 446, 797
HpyF3I CTNAG 3 cut(s) 297, 588, 614
HpySE526I ACGT 2 cut(s) 834, 1271
Hsp92I GRCGYC 2 cut(s) 23, 834
Hsp92II CATG 9 cut(s) 380, 460, 527, 662, 797, 833, 1037, 1100, 1296
HspAI GCGC 1 cut(s) 166
KpnI GGTACC 1 cut(s) 1203
Ksp22I TGATCA 1 cut(s) 526
Kzo9I GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
LmnI GCTCC 3 cut(s) 139, 389, 1078
Lsp1109I GCAGC 4 cut(s) 145, 241, 263, 959
LweI GCATC 4 cut(s) 436, 554, 787, 992
MaeI CTAG 3 cut(s) 696, 765, 1161
MaeII ACGT 2 cut(s) 834, 1271
MaeIII GTNAC 1 cut(s) 602
MalI GATC 9 cut(s) 36, 411, 528, 559, 763, 916, 1032, 1057, 1146
MboI GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
MboII GAAGA 2 cut(s) 576, 1259
MflI RGATCY 3 cut(s) 409, 557, 761
MhlI GDGCHC 5 cut(s) 72, 264, 464, 483, 703
MluCI AATT 1 cut(s) 443
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 892
MslI CAYNNNNRTG 1 cut(s) 1134
MspA1I CMGCKG 4 cut(s) 136, 254, 773, 950
MspI CCGG 2 cut(s) 704, 1188
MspR9I CCNGG 4 cut(s) 244, 555, 1080, 1298
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 4 cut(s) 244, 555, 1080, 1298
MwoI GCNNNNNNNGC 4 cut(s) 20, 139, 446, 797
NdeII GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
NlaIII CATG 9 cut(s) 380, 460, 527, 662, 797, 833, 1037, 1100, 1296
NlaIV GGNNCC 3 cut(s) 63, 129, 1201
NmeAIII GCCGAG 2 cut(s) 283, 1030
NspI RCATGY 2 cut(s) 1100, 1296
PagI TCATGA 3 cut(s) 523, 793, 1033
PasI CCCWGGG 1 cut(s) 243
PctI GAATGC 1 cut(s) 1141
PfeI GAWTC 4 cut(s) 282, 398, 728, 823
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 1078
PkrI GCNGC 4 cut(s) 135, 253, 256, 949
Ple19I CGATCG 1 cut(s) 37
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PpuMI RGGWCCY 1 cut(s) 1252
Psp5II RGGWCCY 1 cut(s) 1252
Psp6I CCWGG 4 cut(s) 242, 553, 1078, 1296
PspGI CCWGG 4 cut(s) 242, 553, 1078, 1296
PspN4I GGNNCC 3 cut(s) 63, 129, 1201
PspPI GGNCC 4 cut(s) 128, 937, 1239, 1252
PspPPI RGGWCCY 1 cut(s) 1252
PsrI GAACNNNNNNTAC 2 cut(s) 23, 55
PstNI CAGNNNCTG 2 cut(s) 560, 857
PsuI RGATCY 3 cut(s) 409, 557, 761
PvuI CGATCG 1 cut(s) 37
PvuII CAGCTG 4 cut(s) 136, 254, 773, 950
RsaI GTAC 3 cut(s) 963, 1186, 1201
RsaNI GTAC 3 cut(s) 962, 1185, 1200
RseI CAYNNNNRTG 1 cut(s) 1134
SatI GCNGC 4 cut(s) 134, 252, 255, 948
Sau3AI GATC 9 cut(s) 34, 409, 526, 557, 761, 914, 1030, 1055, 1144
Sau96I GGNCC 4 cut(s) 128, 937, 1239, 1252
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 4 cut(s) 244, 555, 1080, 1298
SduI GDGCHC 5 cut(s) 72, 264, 464, 483, 703
SfaNI GCATC 4 cut(s) 436, 554, 787, 992
SfcI CTRYAG 1 cut(s) 712
SinI GGWCC 2 cut(s) 128, 1252
SmiMI CAYNNNNRTG 1 cut(s) 1134
Sse9I AATT 1 cut(s) 443
SsiI CCGC 4 cut(s) 131, 886, 1179, 1216
SspMI CTAG 3 cut(s) 696, 765, 1161
StyD4I CCNGG 4 cut(s) 242, 553, 1078, 1296
TaaI ACNGT 4 cut(s) 227, 332, 670, 966
TaiI ACGT 2 cut(s) 837, 1274
TaqI TCGA 3 cut(s) 37, 430, 1093
TasI AATT 1 cut(s) 443
TfiI GAWTC 4 cut(s) 282, 398, 728, 823
TscAI CASTG 7 cut(s) 53, 72, 202, 331, 510, 577, 971
TseI GCWGC 4 cut(s) 133, 251, 254, 947
TspDTI ATGAA 6 cut(s) 17, 512, 797, 914, 1050, 1118
TspGWI ACGGA 2 cut(s) 32, 1137
TspRI CASTG 7 cut(s) 53, 72, 202, 331, 510, 577, 971
Van91I CCANNNNNTGG 1 cut(s) 560
VneI GTGCAC 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 128, 1252
XapI RAATTY 1 cut(s) 443
XceI RCATGY 2 cut(s) 1100, 1296
XspI CTAG 3 cut(s) 696, 765, 1161
ZraI GACGTC 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.