Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0003346

Physical Location & Seq

Genomic Coordinates Forward (+)

85802045 .. 85807175

Gene Structure 5131 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00148140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGAAACTCCGTCGCAGCAGCAGCAACAGCAGCAACCTCCGGCGAAGAAGAAGGAGACCCGGGGCCGGAAGCCCAAGCCCAAGGAGGACAAGGAGGCCCACTCGGCTTCGAAAATGAAGAAGGCTCAGCAGCAACAACAGCAGCAGCAGCAGCAGCACAGCGTCGACGAGAAGTACAGTCAGTGGAAGTCGCTCGTCCCTGTCCTCTACGACTGGCTTGCCAACCACAACCTCGTCTGGCCCTCCCTCTCTTGCCGGTGGGGCCCCCAGCTCGAGCAGGCTACCTACAAGAATCGCCAGCGGCTCTACCTCTCTGAGCAGACTGATGGCAGTGTTCCGAATACTCTGGTTATTGCGAATTGCGAGGTTGTCAAACGTAGGGTTGCCGCTGCGGAGCACATATCTCAGTTCAATGAAGAAGCGCGCTCACCATTTGTGAAGAAGTACAAGACCATCATACATCCTGGGGAGGTAAACAGAATCAGAGAGCTTCCACAGAACACTAAGATAGTGGCAACGCATACTGACAGTCCTGATGTCCTTATTTGGGATGTTGAAGCTCAACCTAACCGTCATGCTGTCTTTGGGGCTACAAACTCTCGTCCGGATTTGATTTTGACTGGACATCAAGATAATGCAGAATTTGCTCTTGCAATGTGTCCAACTGAACCCTATGTGCTCTCTGGAGGTAAGGACAAAACAGTGGTTCTGTGGAGTATTCAGGACCATATAGCAGCATCTACCACAGATCCAGCAGCAACCAAGTCTCCAGGATCTGGTGGATCAATTATTAAGCCCGGGGATGGTAATGAAAAGACTGCTGATGGTCCTTCTGTTGCACCAAGGGGTGTCTACCAGGGGCATGAGGATACAGTGGAAGATGTGGCATTTTGCCCAACTAGCTCACAGGAGTTCTGTAGTGTTGGTGACGATGCCTGCCTTATACTATGGGATGCACGTGTGGGTTCTAGCCCTGCTGTAAAGGTTGAAAAAGCGCACGATGCTGATCTTCACTGTGTTGATTGGAATCCCCATGATGATAACCTTATCCTAACAGGGTCAGCAGATCATTCTGTTCGCATGTTTGATCGGCGGAATCTTACTTCTGGTGGAGTTGGTGCACCTATTTATAAATTTGAGGGTCATAAAGCTGCTGTTCTCTGTGTCCAGTGGTCTCCAGACAAATCATCCATCTTTGGAAGTTCTGCAGAGGATGGTCTTTTAAACATTTGGGATTATGAGAAGGTCAGTAAGAAGGAGCGAACTTCCAAGAACCCAAGTTCTCCTCCTGGTTTGTTTTTCCAGCATGCAGGGCACAGGGACAAAGTTGTTGATTTTCATTGGAATGCATCTGACCCATGGACTGTTGTTAGTGTGTCTGATGACTGTGATACTACTGGTGGAGGGGGGACATTGCAGATATGGCGCATGAGTGATTTGATCTACAGGCCTGAAGAGGAGGTTTTGGAAGAGCTTGAGAAGTTCAAGTCACATGTTATTTCTTGTGCAAAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009892 GO:0009987 GO:0010224 GO:0010468 GO:0010605 GO:0010629 GO:0019222 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046872 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

CAF1C_H4-bd WD40

Protein Analysis

505

Amino Acids

56.13

Weight (kDa)

6.03

Isoelectric Point (pI)

50.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1C_H4-bd	PF12265	57 - 124	9e-20	Histone-binding protein RBBP4 or subunit C of CAF1 complex
Beta-prop_TEP1_2nd	PF25047	278 - 417	2.4e-10	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	283 - 364	1.9e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	284 - 416	2e-19	WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	284 - 365	5.4e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	285 - 324	8.1e-06	THOC3 beta-propeller domain
WDR55	PF24796	286 - 416	6.6e-07	WDR55
Beta-prop_EIPR1	PF23609	288 - 362	3.9e-09	EIPR1 beta-propeller
Beta-prop_EML	PF23409	288 - 418	6.5e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_Aladin	PF25460	291 - 415	2.7e-06	Aladin seven-bladed propeller
Beta-prop_EML_2	PF23414	301 - 427	2.2e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	315 - 419	9.2e-10	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	331 - 461	1.1e-10	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	333 - 423	7e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	335 - 421	4.3e-07	WDHD1 first WD40 domain
WD40	PF00400	374 - 412	2.7e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19520 AT4G29730 AT4G29730 AT4G29730
fragaria_vesca	FvH4_1g07500 FvH4_6g33340 FvH4_6g33340
malus_domestica	MD02G1079600.v1.1 MD09G1193500.v1.1 MD15G1207100.v1.1
prunus_persica	Prupe.3G049900_v2.0.a1 Prupe.7G209600_v2.0.a1 Prupe.7G209600_v2.0.a1
pyrus_communis	pycom02g06340 pycom09g11000 pycom15g18350
rosa_chinensis	RchiOBHm_Chr2g0093391 RchiOBHm_Chr2g0143191
rosa_laevigata	RLG00000016395 RLG00000020012
rosa_multiflora	Rmu_sc0005227.1_g000015 Rmu_sc0032382.1_g000001 Rmu_ssc0000301.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102060 Rroxscaffold_2G00148140
rosa_rugosa	Rorug02G0035100 Rorug02G0379000
rosa_samantha	Rh2AG431800 Rh2BG082700 Rh2BG440400 Rh2CG084700 Rh2CG417700 Rh2DG080400 Rh2DG450600
rosa_wichuraiana	Rw2G006520 Rw2G035280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1131
AccB7I	CCANNNNNTGG	1 cut(s)	776
AccI	GTMKAC	2 cut(s)	165, 852
AccII	CGCG	1 cut(s)	424
AccIII	TCCGGA	1 cut(s)	604
AciI	CCGC	4 cut(s)	301, 387, 392, 1093
AclWI	GGATC	3 cut(s)	743, 781, 790
AcsI	RAATTY	2 cut(s)	641, 1133
AcuI	CTGAAG	1 cut(s)	1473
AcvI	CACGTG	1 cut(s)	959
AfaI	GTAC	2 cut(s)	176, 446
AfiI	CCNNNNNNNGG	4 cut(s)	66, 547, 776, 803
AflIII	ACRYGT	2 cut(s)	958, 1492
AgsI	TTSAA	4 cut(s)	412, 557, 989, 1486
AjnI	CCWGG	4 cut(s)	463, 769, 855, 1288
AluBI	AGCT	6 cut(s)	271, 490, 560, 903, 1151, 1474
AluI	AGCT	6 cut(s)	271, 490, 560, 903, 1151, 1474
Alw21I	GWGCWC	3 cut(s)	399, 681, 1123
Alw26I	GTCTC	3 cut(s)	50, 771, 1179
Alw44I	GTGCAC	1 cut(s)	1119
AlwI	GGATC	3 cut(s)	743, 781, 790
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	3 cut(s)	60, 272, 797
Aor13HI	TCCGGA	1 cut(s)	604
AoxI	GGCC	5 cut(s)	64, 96, 239, 262, 1448
ApaI	GGGCCC	1 cut(s)	266
ApaLI	GTGCAC	1 cut(s)	1119
ApoI	RAATTY	2 cut(s)	641, 1133
AspLEI	GCGC	4 cut(s)	424, 426, 997, 1428
AspS9I	GGNCC	7 cut(s)	64, 97, 240, 262, 263, 724, 827
AsuC2I	CCSGG	4 cut(s)	61, 62, 798, 799
AsuHPI	GGTGA	2 cut(s)	420, 938
AsuII	TTCGAA	1 cut(s)	110
AvaI	CYCGRG	3 cut(s)	60, 272, 797
AvaII	GGWCC	2 cut(s)	724, 827
BaeGI	GKGCMC	3 cut(s)	266, 1123, 1317
BanII	GRGCYC	1 cut(s)	266
BbrPI	CACGTG	1 cut(s)	959
Bbv12I	GWGCWC	3 cut(s)	399, 681, 1123
BccI	CCATC	6 cut(s)	320, 461, 797, 818, 1199, 1208
BcgI	CGANNNNNNTGC	2 cut(s)	200, 234
BciT130I	CCWGG	4 cut(s)	465, 771, 857, 1290
BciVI	GTATCC	1 cut(s)	862
BcnI	CCSGG	4 cut(s)	61, 62, 798, 799
BcoDI	GTCTC	3 cut(s)	50, 771, 1179
BfaI	CTAG	2 cut(s)	900, 969
BfmI	CTRYAG	3 cut(s)	916, 1206, 1444
BfuI	GTATCC	1 cut(s)	862
BglI	GCCNNNNNGGC	2 cut(s)	104, 261
BlpI	GCTNAGC	1 cut(s)	126
Bme1390I	CCNGG	8 cut(s)	61, 62, 465, 771, 798, 799, 857, 1290
Bme18I	GGWCC	2 cut(s)	724, 827
BmeT110I	CYCGRG	3 cut(s)	60, 272, 797
BmgT120I	GGNCC	7 cut(s)	64, 97, 240, 262, 263, 724, 827
BmiI	GGNNCC	4 cut(s)	65, 263, 264, 265
BmrFI	CCNGG	8 cut(s)	61, 62, 465, 771, 798, 799, 857, 1290
BmsI	GCATC	5 cut(s)	746, 922, 943, 991, 1358
BplI	GAGNNNNNCTC	2 cut(s)	86, 118
BpmI	CTGGAG	3 cut(s)	705, 753, 1161
Bpu1102I	GCTNAGC	1 cut(s)	126
Bpu14I	TTCGAA	1 cut(s)	110
BpuEI	CTTGAG	1 cut(s)	1496
BpuMI	CCSGG	4 cut(s)	61, 62, 798, 799
BsaAI	YACGTR	1 cut(s)	959
BsaBI	GATNNNNATC	2 cut(s)	1005, 1026
BsaI	GGTCTC	2 cut(s)	50, 1179
BsaJI	CCNNGG	9 cut(s)	60, 61, 81, 464, 797, 798, 842, 856, 1358
BsaWI	WCCGGW	1 cut(s)	604
BsaXI	ACNNNNNCTCC	2 cut(s)	751, 781
Bsc4I	CCNNNNNNNGG	4 cut(s)	66, 547, 776, 803
Bse118I	RCCGGY	1 cut(s)	255
Bse1I	ACTGG	4 cut(s)	218, 625, 1168, 1402
Bse3DI	GCAATG	2 cut(s)	660, 1412
Bse8I	GATNNNNATC	2 cut(s)	1005, 1026
BseAI	TCCGGA	1 cut(s)	604
BseBI	CCWGG	4 cut(s)	465, 771, 857, 1290
BseDI	CCNNGG	9 cut(s)	60, 61, 81, 464, 797, 798, 842, 856, 1358
BseGI	GGATG	6 cut(s)	460, 556, 808, 958, 1187, 1219
BseJI	GATNNNNATC	2 cut(s)	1005, 1026
BseLI	CCNNNNNNNGG	4 cut(s)	66, 547, 776, 803
BseMI	GCAATG	2 cut(s)	660, 1412
BseMII	CTCAG	3 cut(s)	140, 306, 419
BseNI	ACTGG	4 cut(s)	218, 625, 1168, 1402
BsePI	GCGCGC	1 cut(s)	422
BseRI	GAGGAG	2 cut(s)	1275, 1472
BseSI	GKGCMC	3 cut(s)	266, 1123, 1317
BseYI	CCCAGC	1 cut(s)	267
Bsh1236I	CGCG	1 cut(s)	424
BshFI	GGCC	5 cut(s)	66, 98, 241, 264, 1450
BsiHKAI	GWGCWC	3 cut(s)	399, 681, 1123
BsiHKCI	CYCGRG	3 cut(s)	60, 272, 797
BsiSI	CCGG	6 cut(s)	41, 61, 67, 256, 605, 798
BslFI	GGGAC	3 cut(s)	182, 1334, 1423
BslI	CCNNNNNNNGG	4 cut(s)	66, 547, 776, 803
BsmAI	GTCTC	3 cut(s)	50, 771, 1179
BsmFI	GGGAC	3 cut(s)	182, 1334, 1423
BsmI	GAATGC	1 cut(s)	1351
BsnI	GGCC	5 cut(s)	66, 98, 241, 264, 1450
Bso31I	GGTCTC	2 cut(s)	50, 1179
BsoBI	CYCGRG	3 cut(s)	60, 272, 797
Bsp119I	TTCGAA	1 cut(s)	110
Bsp120I	GGGCCC	1 cut(s)	262
Bsp1286I	GDGCHC	5 cut(s)	266, 399, 681, 1123, 1317
Bsp13I	TCCGGA	1 cut(s)	604
Bsp143I	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
Bsp1720I	GCTNAGC	1 cut(s)	126
Bsp19I	CCATGG	1 cut(s)	1358
BspACI	CCGC	4 cut(s)	301, 387, 392, 1093
BspANI	GGCC	5 cut(s)	66, 98, 241, 264, 1450
BspCNI	CTCAG	3 cut(s)	139, 307, 418
BspEI	TCCGGA	1 cut(s)	604
BspFNI	CGCG	1 cut(s)	424
BspLI	GGNNCC	4 cut(s)	65, 263, 264, 265
BspMAI	CTGCAG	1 cut(s)	1210
BspPI	GGATC	3 cut(s)	743, 781, 790
BspQI	GCTCTTC	1 cut(s)	1464
BspT104I	TTCGAA	1 cut(s)	110
BspTNI	GGTCTC	2 cut(s)	50, 1179
BsrDI	GCAATG	2 cut(s)	660, 1412
BsrFI	RCCGGY	1 cut(s)	255
BsrI	ACTGG	4 cut(s)	218, 625, 1168, 1402
BssAI	RCCGGY	1 cut(s)	255
BssECI	CCNNGG	9 cut(s)	60, 61, 81, 464, 797, 798, 842, 856, 1358
BssHII	GCGCGC	1 cut(s)	422
BssMI	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
BssT1I	CCWWGG	3 cut(s)	81, 842, 1358
Bst2UI	CCWGG	4 cut(s)	465, 771, 857, 1290
Bst4CI	ACNGT	8 cut(s)	179, 530, 572, 703, 874, 1016, 1366, 1388
Bst6I	CTCTTC	2 cut(s)	1449, 1464
BstAPI	GCANNNNNTGC	1 cut(s)	644
BstBAI	YACGTR	1 cut(s)	959
BstBI	TTCGAA	1 cut(s)	110
BstC8I	GCNNGC	6 cut(s)	219, 279, 299, 424, 937, 1308
BstDEI	CTNAG	4 cut(s)	126, 315, 405, 504
BstDSI	CCRYGG	1 cut(s)	1358
BstF5I	GGATG	6 cut(s)	460, 556, 808, 958, 1187, 1219
BstFNI	CGCG	1 cut(s)	424
BstHHI	GCGC	4 cut(s)	424, 426, 997, 1428
BstKTI	GATC	7 cut(s)	751, 776, 785, 1009, 1069, 1090, 1443
BstMAI	GTCTC	3 cut(s)	50, 771, 1179
BstMBI	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
BstNI	CCWGG	4 cut(s)	465, 771, 857, 1290
BstNSI	RCATGY	3 cut(s)	1084, 1310, 1496
BstSCI	CCNGG	8 cut(s)	59, 60, 463, 769, 796, 797, 855, 1288
BstSFI	CTRYAG	3 cut(s)	916, 1206, 1444
BstSLI	GKGCMC	3 cut(s)	266, 1123, 1317
BstUI	CGCG	1 cut(s)	424
BstX2I	RGATCY	2 cut(s)	748, 773
BstYI	RGATCY	2 cut(s)	748, 773
BsuI	GTATCC	1 cut(s)	862
BsuRI	GGCC	5 cut(s)	66, 98, 241, 264, 1450
BtgI	CCRYGG	1 cut(s)	1358
BtsCI	GGATG	6 cut(s)	460, 556, 808, 958, 1187, 1219
BtsI	GCAGTG	1 cut(s)	337
BtsIMutI	CAGTG	6 cut(s)	188, 337, 708, 879, 1012, 1175
Cac8I	GCNNGC	6 cut(s)	219, 279, 299, 424, 937, 1308
CaiI	CAGNNNCTG	1 cut(s)	776
CfoI	GCGC	4 cut(s)	424, 426, 997, 1428
Cfr10I	RCCGGY	1 cut(s)	255
Cfr13I	GGNCC	7 cut(s)	64, 97, 240, 262, 263, 724, 827
Cfr9I	CCCGGG	2 cut(s)	60, 797
CseI	GACGC	1 cut(s)	151
Csp6I	GTAC	2 cut(s)	175, 445
CviAII	CATG	8 cut(s)	575, 863, 1034, 1081, 1307, 1359, 1429, 1493
CviQI	GTAC	2 cut(s)	175, 445
DdeI	CTNAG	4 cut(s)	126, 315, 405, 504
DpnI	GATC	7 cut(s)	750, 775, 784, 1008, 1068, 1089, 1442
DpnII	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
DraI	TTTAAA	1 cut(s)	1224
Eam1104I	CTCTTC	2 cut(s)	1449, 1464
EarI	CTCTTC	2 cut(s)	1449, 1464
EciI	GGCGGA	1 cut(s)	1108
Eco130I	CCWWGG	3 cut(s)	81, 842, 1358
Eco147I	AGGCCT	1 cut(s)	1450
Eco24I	GRGCYC	1 cut(s)	266
Eco31I	GGTCTC	2 cut(s)	50, 1179
Eco47I	GGWCC	2 cut(s)	724, 827
Eco57I	CTGAAG	1 cut(s)	1473
Eco72I	CACGTG	1 cut(s)	959
Eco88I	CYCGRG	3 cut(s)	60, 272, 797
EcoO109I	RGGNCCY	2 cut(s)	262, 263
EcoRII	CCWGG	4 cut(s)	463, 769, 855, 1288
EcoT14I	CCWWGG	3 cut(s)	81, 842, 1358
EcoT22I	ATGCAT	1 cut(s)	1351
EcoT38I	GRGCYC	1 cut(s)	266
ErhI	CCWWGG	3 cut(s)	81, 842, 1358
FaeI	CATG	8 cut(s)	578, 866, 1037, 1084, 1310, 1362, 1432, 1496
FaqI	GGGAC	3 cut(s)	182, 1334, 1423
FatI	CATG	8 cut(s)	574, 862, 1033, 1080, 1306, 1358, 1428, 1492
FblI	GTMKAC	2 cut(s)	165, 852
FokI	GGATG	6 cut(s)	447, 563, 815, 965, 1174, 1226
FriOI	GRGCYC	1 cut(s)	266
FspBI	CTAG	2 cut(s)	900, 969
GlaI	GCGC	4 cut(s)	423, 425, 996, 1427
GsaI	CCCAGC	1 cut(s)	271
GsuI	CTGGAG	3 cut(s)	705, 753, 1161
HaeIII	GGCC	5 cut(s)	66, 98, 241, 264, 1450
HapII	CCGG	6 cut(s)	41, 61, 67, 256, 605, 798
HgaI	GACGC	1 cut(s)	151
HhaI	GCGC	4 cut(s)	424, 426, 997, 1428
Hin1II	CATG	8 cut(s)	578, 866, 1037, 1084, 1310, 1362, 1432, 1496
Hin6I	GCGC	4 cut(s)	422, 424, 995, 1426
HinP1I	GCGC	4 cut(s)	422, 424, 995, 1426
HincII	GTYRAC	1 cut(s)	166
HindII	GTYRAC	1 cut(s)	166
HinfI	GANTC	4 cut(s)	292, 480, 1027, 1096
HpaII	CCGG	6 cut(s)	41, 61, 67, 256, 605, 798
HphI	GGTGA	2 cut(s)	420, 938
Hpy166II	GTNNAC	4 cut(s)	166, 475, 853, 1121
Hpy188I	TCNGA	5 cut(s)	316, 339, 485, 1354, 1381
Hpy188III	TCNNGA	6 cut(s)	533, 605, 629, 684, 722, 1178
Hpy8I	GTNNAC	4 cut(s)	166, 475, 853, 1121
Hpy99I	CGWCG	3 cut(s)	16, 167, 170
HpyAV	CCTTC	5 cut(s)	46, 115, 840, 1237, 1249
HpyCH4III	ACNGT	8 cut(s)	179, 530, 572, 703, 874, 1016, 1366, 1388
HpyCH4IV	ACGT	2 cut(s)	376, 958
HpyF3I	CTNAG	4 cut(s)	126, 315, 405, 504
HpySE526I	ACGT	2 cut(s)	376, 958
Hsp92II	CATG	8 cut(s)	578, 866, 1037, 1084, 1310, 1362, 1432, 1496
HspAI	GCGC	4 cut(s)	422, 424, 995, 1426
Kpn2I	TCCGGA	1 cut(s)	604
Kzo9I	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
LguI	GCTCTTC	1 cut(s)	1464
LmnI	GCTCC	2 cut(s)	394, 1258
LweI	GCATC	5 cut(s)	746, 922, 943, 991, 1358
MaeI	CTAG	2 cut(s)	900, 969
MaeII	ACGT	2 cut(s)	376, 958
MaeIII	GTNAC	2 cut(s)	926, 1488
MalI	GATC	7 cut(s)	750, 775, 784, 1008, 1068, 1089, 1442
MboI	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
MboII	GAAGA	9 cut(s)	58, 61, 130, 428, 451, 890, 1001, 1466, 1481
MflI	RGATCY	2 cut(s)	748, 773
MhlI	GDGCHC	5 cut(s)	266, 399, 681, 1123, 1317
MluCI	AATT	4 cut(s)	358, 641, 786, 1133
MmeI	TCCRAC	1 cut(s)	686
Mph1103I	ATGCAT	1 cut(s)	1351
MroI	TCCGGA	1 cut(s)	604
MseI	TTAA	2 cut(s)	792, 1223
MslI	CAYNNNNRTG	1 cut(s)	1344
MspA1I	CMGCKG	2 cut(s)	301, 389
MspI	CCGG	6 cut(s)	41, 61, 67, 256, 605, 798
MspR9I	CCNGG	8 cut(s)	61, 62, 465, 771, 798, 799, 857, 1290
Mva1269I	GAATGC	1 cut(s)	1351
MvaI	CCWGG	4 cut(s)	465, 771, 857, 1290
MvnI	CGCG	1 cut(s)	424
NciI	CCSGG	4 cut(s)	61, 62, 798, 799
NcoI	CCATGG	1 cut(s)	1358
NdeII	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
NlaIII	CATG	8 cut(s)	578, 866, 1037, 1084, 1310, 1362, 1432, 1496
NlaIV	GGNNCC	4 cut(s)	65, 263, 264, 265
NmeAIII	GCCGAG	1 cut(s)	83
NmuCI	GTSAC	2 cut(s)	926, 1488
NsiI	ATGCAT	1 cut(s)	1351
NspI	RCATGY	3 cut(s)	1084, 1310, 1496
NspV	TTCGAA	1 cut(s)	110
PaeI	GCATGC	1 cut(s)	1310
PaeR7I	CTCGAG	1 cut(s)	272
PauI	GCGCGC	1 cut(s)	422
PceI	AGGCCT	1 cut(s)	1450
PciI	ACATGT	1 cut(s)	1492
PciSI	GCTCTTC	1 cut(s)	1464
PctI	GAATGC	1 cut(s)	1351
PfeI	GAWTC	4 cut(s)	292, 480, 1027, 1096
PflMI	CCANNNNNTGG	1 cut(s)	776
PfoI	TCCNGGA	1 cut(s)	769
PmaCI	CACGTG	1 cut(s)	959
PmlI	CACGTG	1 cut(s)	959
Ppu21I	YACGTR	1 cut(s)	959
PscI	ACATGT	1 cut(s)	1492
PsiI	TTATAA	1 cut(s)	1131
Psp6I	CCWGG	4 cut(s)	463, 769, 855, 1288
PspCI	CACGTG	1 cut(s)	959
PspFI	CCCAGC	1 cut(s)	267
PspGI	CCWGG	4 cut(s)	463, 769, 855, 1288
PspN4I	GGNNCC	4 cut(s)	65, 263, 264, 265
PspOMI	GGGCCC	1 cut(s)	262
PspPI	GGNCC	7 cut(s)	64, 97, 240, 262, 263, 724, 827
PspXI	VCTCGAGB	1 cut(s)	272
PstI	CTGCAG	1 cut(s)	1210
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	2 cut(s)	748, 773
PteI	GCGCGC	1 cut(s)	422
RsaI	GTAC	2 cut(s)	176, 446
RsaNI	GTAC	2 cut(s)	175, 445
RseI	CAYNNNNRTG	1 cut(s)	1344
SalI	GTCGAC	1 cut(s)	164
SapI	GCTCTTC	1 cut(s)	1464
SaqAI	TTAA	2 cut(s)	792, 1223
Sau3AI	GATC	7 cut(s)	748, 773, 782, 1006, 1066, 1087, 1440
Sau96I	GGNCC	7 cut(s)	64, 97, 240, 262, 263, 724, 827
ScrFI	CCNGG	8 cut(s)	61, 62, 465, 771, 798, 799, 857, 1290
SduI	GDGCHC	5 cut(s)	266, 399, 681, 1123, 1317
SfaNI	GCATC	5 cut(s)	746, 922, 943, 991, 1358
SfcI	CTRYAG	3 cut(s)	916, 1206, 1444
Sfr274I	CTCGAG	1 cut(s)	272
SfuI	TTCGAA	1 cut(s)	110
SgrDI	CGTCGACG	1 cut(s)	164
SinI	GGWCC	2 cut(s)	724, 827
SlaI	CTCGAG	1 cut(s)	272
SmaI	CCCGGG	2 cut(s)	62, 799
SmiMI	CAYNNNNRTG	1 cut(s)	1344
SmlI	CTYRAG	2 cut(s)	272, 1475
SmoI	CTYRAG	2 cut(s)	272, 1475
SphI	GCATGC	1 cut(s)	1310
Sse9I	AATT	4 cut(s)	358, 641, 786, 1133
SseBI	AGGCCT	1 cut(s)	1450
SsiI	CCGC	4 cut(s)	301, 387, 392, 1093
SspMI	CTAG	2 cut(s)	900, 969
StuI	AGGCCT	1 cut(s)	1450
StyD4I	CCNGG	8 cut(s)	59, 60, 463, 769, 796, 797, 855, 1288
StyI	CCWWGG	3 cut(s)	81, 842, 1358
TaaI	ACNGT	8 cut(s)	179, 530, 572, 703, 874, 1016, 1366, 1388
TaiI	ACGT	2 cut(s)	379, 961
TaqI	TCGA	3 cut(s)	110, 165, 273
TasI	AATT	4 cut(s)	358, 641, 786, 1133
TatI	WGTACW	2 cut(s)	174, 444
TauI	GCSGC	2 cut(s)	304, 389
TfiI	GAWTC	4 cut(s)	292, 480, 1027, 1096
Tru1I	TTAA	2 cut(s)	792, 1223
Tru9I	TTAA	2 cut(s)	792, 1223
TscAI	CASTG	6 cut(s)	188, 337, 708, 879, 1019, 1175
TseFI	GTSAC	2 cut(s)	926, 1488
Tsp45I	GTSAC	2 cut(s)	926, 1488
TspDTI	ATGAA	4 cut(s)	131, 429, 825, 1328
TspMI	CCCGGG	2 cut(s)	60, 797
TspRI	CASTG	6 cut(s)	188, 337, 708, 879, 1019, 1175
Van91I	CCANNNNNTGG	1 cut(s)	776
VneI	GTGCAC	1 cut(s)	1119
VpaK11BI	GGWCC	2 cut(s)	724, 827
XapI	RAATTY	2 cut(s)	641, 1133
XceI	RCATGY	3 cut(s)	1084, 1310, 1496
XhoI	CTCGAG	1 cut(s)	272
XmaI	CCCGGG	2 cut(s)	60, 797
XmiI	GTMKAC	2 cut(s)	165, 852
XspI	CTAG	2 cut(s)	900, 969
Zsp2I	ATGCAT	1 cut(s)	1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.