Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003346

Physical Location & Seq

Genomic Coordinates Forward (+)

6289982 .. 6295726

Gene Structure 5745 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G006520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGAAACTCCGTCGCAGCAGCAGCAACCTCCGGCGAAGAAGAAGGAGACCCGGGGCCGGAAGCCCAAGCCCAAGGAGGACAAGGAGGCCCACTCGGCTTCGAAAATGAAGAAGGCTCAGCAGCAACAACAGCAGCAGCAGCAGCACAGCGTCGACGAGAAGTACAGTCAGTGGAAGTCGCTCGTCCCTGTCCTCTACGACTGGCTTGCCAACCACAACCTCGTCTGGCCCTCCCTCTCTTGCCGGTGGGGCCCGCAGCTCGAGCAGGCTACCTACAAGAATCGCCAGCGGCTCTACCTCTCTGAGCAGACTGATGGCAGTGTTCCGAATACTCTGGTTATTGCAAATTGCGAGGTTGTCAAACGTAGAGTTGCCGCTGCGGAGCACATATCTCAGTTCAATGAAGAAGCGCGCTCACCATTTGTGAAGAAGTACAAGACCATCATACATCCTGGGGAGGTAAACAGAATCAGAGAGCTTCCACAGAACACTAAGATAGTGGCAACGCATACTGACAGTCCTGATGTCCTTATTTGGGATGTTGAAGCTCAACCTAACCGTCATGCTGTCTTTGGGGCTACAAACTCTCGTCCCGATTTGATTTTGACTGGACATCAAGATAATGCAGAATTTGCTCTTGCAATGTGTCCAACTGAACCCTATGTGCTCTCTGGAGGTAAGGACAAAACAGTGGTTCTGTGGAGTATTCAGGACCATATAGCAGCATCTACCACAGATCCAGCTGCAACCAAGTCTCCAGGATCTGGTGGATCAATTATTAAGCCTGGGGATGGTAATGAAAAGACTGCTGATGGTCCTTCTGTTTCACCAAGGGGTGTCTACCAGGGGCACGAGGATACAGTGGAAGATGTGGCATTTTGCCCAACTAGCTCACAGGAGTTCTGTAGTGTTGGTGACGATGCCTGCCTTATACTATGGGATGCACGTGTGGGTTCTAGTCCTGCTGTAAAGGTTGAAAAAGCGCACGATGCTGATCTTCACTGTGTTGATTGGAATCCCCATGATGATAACCTTATCCTAACAGGGTCAGCAGATCATTCTGTTCGCATGTTTGATCGGCGGAATCTTACTTCTGGTGGAGTTGGCGCACCTATTTATAAATTTGAGGGTCATAAAGCTGCTGTTCTCTGTGTCCAGTGGTCTCCAGACAAATCATCCATCTTTGGAAGTTCTGCAGAGGATGGTCTTTTAAACATTTGGGATTATGAGAAGGTCAGTAAGAAGGAGCGAACTTCCAAGAACCCAAGTTCTCCTCCTGGTTTGTTTTTCCAGCATGCAGGGCACAGGGACAAAGTTGTTGATTTTCATTGGAATGCATCTGACCCATGGACTGTTGTTAGTGTGTCTGATGACTGTGATACTACTGGTGGAGGGGGGACATTGCAGATATGGCGCATGAGTGATTTGATCTACAGGCCTGAAGAGGAGGTTTTGGAAGAGCTTGAGAAGTTCAAGTCACATGTTATTTCTTGTGCGAAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009892 GO:0009987 GO:0010224 GO:0010468 GO:0010605 GO:0010629 GO:0019222 GO:0031461 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046872 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04218 map04218

Pfam Domains

Protein Families

CAF1C_H4-bd WD40

Protein Analysis

501

Amino Acids

55.63

Weight (kDa)

6.03

Isoelectric Point (pI)

49.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CAF1C_H4-bd	PF12265	53 - 120	8.9e-20	Histone-binding protein RBBP4 or subunit C of CAF1 complex
Beta-prop_TEP1_2nd	PF25047	274 - 413	1.2e-10	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	279 - 360	1.8e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	280 - 412	1.8e-19	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	280 - 320	7.1e-06	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	280 - 361	5.5e-06	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	282 - 412	6.5e-07	WDR55
Beta-prop_EML	PF23409	284 - 414	6.4e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EIPR1	PF23609	284 - 358	3.7e-09	EIPR1 beta-propeller
Beta-prop_Aladin	PF25460	287 - 411	2.7e-06	Aladin seven-bladed propeller
Beta-prop_EML_2	PF23414	295 - 423	2e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	325 - 415	1.7e-08	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	327 - 457	1.1e-10	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	329 - 419	6.9e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	331 - 417	3.6e-07	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	346 - 413	1.1e-06	CDC20/Fizzy WD40 domain
WD40	PF00400	370 - 408	2.7e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19520 AT4G29730 AT4G29730 AT4G29730
fragaria_vesca	FvH4_1g07500 FvH4_6g33340 FvH4_6g33340
malus_domestica	MD02G1079600.v1.1 MD09G1193500.v1.1 MD15G1207100.v1.1
prunus_persica	Prupe.3G049900_v2.0.a1 Prupe.7G209600_v2.0.a1 Prupe.7G209600_v2.0.a1
pyrus_communis	pycom02g06340 pycom09g11000 pycom15g18350
rosa_chinensis	RchiOBHm_Chr2g0093391 RchiOBHm_Chr2g0143191
rosa_laevigata	RLG00000016395 RLG00000020012
rosa_multiflora	Rmu_sc0005227.1_g000015 Rmu_sc0032382.1_g000001 Rmu_ssc0000301.1_g000001
rosa_roxburghii	Rroxscaffold_2G00102060 Rroxscaffold_2G00148140
rosa_rugosa	Rorug02G0035100 Rorug02G0379000
rosa_samantha	Rh2AG431800 Rh2BG082700 Rh2BG440400 Rh2CG084700 Rh2CG417700 Rh2DG080400 Rh2DG450600
rosa_wichuraiana	Rw2G006520 Rw2G035280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1119
AccB7I	CCANNNNNTGG	1 cut(s)	764
AccI	GTMKAC	2 cut(s)	153, 840
AccII	CGCG	1 cut(s)	412
AciI	CCGC	5 cut(s)	254, 289, 375, 380, 1081
AclWI	GGATC	3 cut(s)	731, 769, 778
AcsI	RAATTY	2 cut(s)	629, 1121
AcuI	CTGAAG	1 cut(s)	1461
AcvI	CACGTG	1 cut(s)	947
AfaI	GTAC	2 cut(s)	164, 434
AfiI	CCNNNNNNNGG	4 cut(s)	57, 535, 764, 791
AflIII	ACRYGT	2 cut(s)	946, 1480
AgsI	TTSAA	4 cut(s)	400, 545, 977, 1474
AjnI	CCWGG	5 cut(s)	451, 757, 784, 843, 1276
AluBI	AGCT	7 cut(s)	259, 478, 548, 743, 891, 1139, 1462
AluI	AGCT	7 cut(s)	259, 478, 548, 743, 891, 1139, 1462
Alw21I	GWGCWC	2 cut(s)	387, 669
Alw26I	GTCTC	3 cut(s)	41, 759, 1167
AlwI	GGATC	3 cut(s)	731, 769, 778
AlwNI	CAGNNNCTG	1 cut(s)	764
Ama87I	CYCGRG	2 cut(s)	51, 260
AoxI	GGCC	5 cut(s)	55, 87, 227, 250, 1436
ApaI	GGGCCC	1 cut(s)	254
ApoI	RAATTY	2 cut(s)	629, 1121
AspLEI	GCGC	5 cut(s)	412, 414, 985, 1109, 1416
AspS9I	GGNCC	7 cut(s)	55, 88, 228, 250, 251, 712, 815
AsuC2I	CCSGG	2 cut(s)	52, 53
AsuHPI	GGTGA	3 cut(s)	408, 819, 926
AsuII	TTCGAA	1 cut(s)	101
AvaI	CYCGRG	2 cut(s)	51, 260
AvaII	GGWCC	2 cut(s)	712, 815
BaeGI	GKGCMC	3 cut(s)	254, 852, 1305
BanII	GRGCYC	1 cut(s)	254
BauI	CACGAG	1 cut(s)	851
BbrPI	CACGTG	1 cut(s)	947
Bbv12I	GWGCWC	2 cut(s)	387, 669
BccI	CCATC	6 cut(s)	308, 449, 785, 806, 1187, 1196
BcgI	CGANNNNNNTGC	2 cut(s)	188, 222
BciT130I	CCWGG	5 cut(s)	453, 759, 786, 845, 1278
BciVI	GTATCC	1 cut(s)	850
BcnI	CCSGG	2 cut(s)	52, 53
BcoDI	GTCTC	3 cut(s)	41, 759, 1167
BfaI	CTAG	2 cut(s)	888, 957
BfmI	CTRYAG	3 cut(s)	904, 1194, 1432
BfuI	GTATCC	1 cut(s)	850
BglI	GCCNNNNNGGC	2 cut(s)	95, 249
BlpI	GCTNAGC	1 cut(s)	117
Bme1390I	CCNGG	7 cut(s)	52, 53, 453, 759, 786, 845, 1278
Bme18I	GGWCC	2 cut(s)	712, 815
BmeT110I	CYCGRG	2 cut(s)	51, 260
BmgT120I	GGNCC	7 cut(s)	55, 88, 228, 250, 251, 712, 815
BmiI	GGNNCC	3 cut(s)	56, 251, 252
BmrFI	CCNGG	7 cut(s)	52, 53, 453, 759, 786, 845, 1278
BmsI	GCATC	5 cut(s)	734, 910, 931, 979, 1346
BplI	GAGNNNNNCTC	2 cut(s)	77, 109
BpmI	CTGGAG	3 cut(s)	693, 741, 1149
Bpu1102I	GCTNAGC	1 cut(s)	117
Bpu14I	TTCGAA	1 cut(s)	101
BpuEI	CTTGAG	1 cut(s)	1484
BpuMI	CCSGG	2 cut(s)	52, 53
BsaAI	YACGTR	1 cut(s)	947
BsaBI	GATNNNNATC	2 cut(s)	993, 1014
BsaI	GGTCTC	2 cut(s)	41, 1167
BsaJI	CCNNGG	8 cut(s)	51, 52, 72, 452, 785, 830, 844, 1346
BsaXI	ACNNNNNCTCC	2 cut(s)	739, 769
Bsc4I	CCNNNNNNNGG	4 cut(s)	57, 535, 764, 791
Bse118I	RCCGGY	1 cut(s)	243
Bse1I	ACTGG	4 cut(s)	206, 613, 1156, 1390
Bse3DI	GCAATG	2 cut(s)	648, 1400
Bse8I	GATNNNNATC	2 cut(s)	993, 1014
BseBI	CCWGG	5 cut(s)	453, 759, 786, 845, 1278
BseDI	CCNNGG	8 cut(s)	51, 52, 72, 452, 785, 830, 844, 1346
BseGI	GGATG	6 cut(s)	448, 544, 796, 946, 1175, 1207
BseJI	GATNNNNATC	2 cut(s)	993, 1014
BseLI	CCNNNNNNNGG	4 cut(s)	57, 535, 764, 791
BseMI	GCAATG	2 cut(s)	648, 1400
BseMII	CTCAG	3 cut(s)	131, 294, 407
BseNI	ACTGG	4 cut(s)	206, 613, 1156, 1390
BsePI	GCGCGC	1 cut(s)	410
BseRI	GAGGAG	2 cut(s)	1263, 1460
BseSI	GKGCMC	3 cut(s)	254, 852, 1305
Bsh1236I	CGCG	1 cut(s)	412
BshFI	GGCC	5 cut(s)	57, 89, 229, 252, 1438
BsiHKAI	GWGCWC	2 cut(s)	387, 669
BsiHKCI	CYCGRG	2 cut(s)	51, 260
BsiSI	CCGG	4 cut(s)	32, 52, 58, 244
BslFI	GGGAC	4 cut(s)	170, 576, 1322, 1411
BslI	CCNNNNNNNGG	4 cut(s)	57, 535, 764, 791
BsmAI	GTCTC	3 cut(s)	41, 759, 1167
BsmFI	GGGAC	4 cut(s)	170, 576, 1322, 1411
BsmI	GAATGC	1 cut(s)	1339
BsnI	GGCC	5 cut(s)	57, 89, 229, 252, 1438
Bso31I	GGTCTC	2 cut(s)	41, 1167
BsoBI	CYCGRG	2 cut(s)	51, 260
Bsp119I	TTCGAA	1 cut(s)	101
Bsp120I	GGGCCC	1 cut(s)	250
Bsp1286I	GDGCHC	5 cut(s)	254, 387, 669, 852, 1305
Bsp143I	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
Bsp1720I	GCTNAGC	1 cut(s)	117
Bsp19I	CCATGG	1 cut(s)	1346
BspACI	CCGC	5 cut(s)	254, 289, 375, 380, 1081
BspANI	GGCC	5 cut(s)	57, 89, 229, 252, 1438
BspCNI	CTCAG	3 cut(s)	130, 295, 406
BspFNI	CGCG	1 cut(s)	412
BspLI	GGNNCC	3 cut(s)	56, 251, 252
BspMAI	CTGCAG	1 cut(s)	1198
BspPI	GGATC	3 cut(s)	731, 769, 778
BspQI	GCTCTTC	1 cut(s)	1452
BspT104I	TTCGAA	1 cut(s)	101
BspTNI	GGTCTC	2 cut(s)	41, 1167
BsrDI	GCAATG	2 cut(s)	648, 1400
BsrFI	RCCGGY	1 cut(s)	243
BsrI	ACTGG	4 cut(s)	206, 613, 1156, 1390
BssAI	RCCGGY	1 cut(s)	243
BssECI	CCNNGG	8 cut(s)	51, 52, 72, 452, 785, 830, 844, 1346
BssHII	GCGCGC	1 cut(s)	410
BssMI	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
BssSI	CACGAG	1 cut(s)	851
BssT1I	CCWWGG	3 cut(s)	72, 830, 1346
Bst2BI	CACGAG	1 cut(s)	851
Bst2UI	CCWGG	5 cut(s)	453, 759, 786, 845, 1278
Bst4CI	ACNGT	8 cut(s)	167, 518, 560, 691, 862, 1004, 1354, 1376
Bst6I	CTCTTC	2 cut(s)	1437, 1452
BstAPI	GCANNNNNTGC	1 cut(s)	632
BstBAI	YACGTR	1 cut(s)	947
BstBI	TTCGAA	1 cut(s)	101
BstC8I	GCNNGC	7 cut(s)	207, 254, 267, 287, 412, 925, 1296
BstDEI	CTNAG	4 cut(s)	117, 303, 393, 492
BstDSI	CCRYGG	1 cut(s)	1346
BstF5I	GGATG	6 cut(s)	448, 544, 796, 946, 1175, 1207
BstFNI	CGCG	1 cut(s)	412
BstHHI	GCGC	5 cut(s)	412, 414, 985, 1109, 1416
BstKTI	GATC	7 cut(s)	739, 764, 773, 997, 1057, 1078, 1431
BstMAI	GTCTC	3 cut(s)	41, 759, 1167
BstMBI	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
BstNI	CCWGG	5 cut(s)	453, 759, 786, 845, 1278
BstNSI	RCATGY	3 cut(s)	1072, 1298, 1484
BstSCI	CCNGG	7 cut(s)	50, 51, 451, 757, 784, 843, 1276
BstSFI	CTRYAG	3 cut(s)	904, 1194, 1432
BstSLI	GKGCMC	3 cut(s)	254, 852, 1305
BstUI	CGCG	1 cut(s)	412
BstX2I	RGATCY	2 cut(s)	736, 761
BstYI	RGATCY	2 cut(s)	736, 761
BsuI	GTATCC	1 cut(s)	850
BsuRI	GGCC	5 cut(s)	57, 89, 229, 252, 1438
BtgI	CCRYGG	1 cut(s)	1346
BtsCI	GGATG	6 cut(s)	448, 544, 796, 946, 1175, 1207
BtsI	GCAGTG	1 cut(s)	325
BtsIMutI	CAGTG	6 cut(s)	176, 325, 696, 867, 1000, 1163
Cac8I	GCNNGC	7 cut(s)	207, 254, 267, 287, 412, 925, 1296
CaiI	CAGNNNCTG	1 cut(s)	764
CfoI	GCGC	5 cut(s)	412, 414, 985, 1109, 1416
Cfr10I	RCCGGY	1 cut(s)	243
Cfr13I	GGNCC	7 cut(s)	55, 88, 228, 250, 251, 712, 815
Cfr9I	CCCGGG	1 cut(s)	51
CseI	GACGC	1 cut(s)	139
Csp6I	GTAC	2 cut(s)	163, 433
CviAII	CATG	7 cut(s)	563, 1022, 1069, 1295, 1347, 1417, 1481
CviQI	GTAC	2 cut(s)	163, 433
DdeI	CTNAG	4 cut(s)	117, 303, 393, 492
DpnI	GATC	7 cut(s)	738, 763, 772, 996, 1056, 1077, 1430
DpnII	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
DraI	TTTAAA	1 cut(s)	1212
Eam1104I	CTCTTC	2 cut(s)	1437, 1452
EarI	CTCTTC	2 cut(s)	1437, 1452
EciI	GGCGGA	1 cut(s)	1096
Eco130I	CCWWGG	3 cut(s)	72, 830, 1346
Eco147I	AGGCCT	1 cut(s)	1438
Eco24I	GRGCYC	1 cut(s)	254
Eco31I	GGTCTC	2 cut(s)	41, 1167
Eco47I	GGWCC	2 cut(s)	712, 815
Eco57I	CTGAAG	1 cut(s)	1461
Eco72I	CACGTG	1 cut(s)	947
Eco88I	CYCGRG	2 cut(s)	51, 260
EcoO109I	RGGNCCY	1 cut(s)	250
EcoRII	CCWGG	5 cut(s)	451, 757, 784, 843, 1276
EcoT14I	CCWWGG	3 cut(s)	72, 830, 1346
EcoT22I	ATGCAT	1 cut(s)	1339
EcoT38I	GRGCYC	1 cut(s)	254
ErhI	CCWWGG	3 cut(s)	72, 830, 1346
FaeI	CATG	7 cut(s)	566, 1025, 1072, 1298, 1350, 1420, 1484
FaqI	GGGAC	4 cut(s)	170, 576, 1322, 1411
FatI	CATG	7 cut(s)	562, 1021, 1068, 1294, 1346, 1416, 1480
FauI	CCCGC	1 cut(s)	261
FblI	GTMKAC	2 cut(s)	153, 840
FokI	GGATG	6 cut(s)	435, 551, 803, 953, 1162, 1214
FriOI	GRGCYC	1 cut(s)	254
FspBI	CTAG	2 cut(s)	888, 957
GlaI	GCGC	5 cut(s)	411, 413, 984, 1108, 1415
GsuI	CTGGAG	3 cut(s)	693, 741, 1149
HaeIII	GGCC	5 cut(s)	57, 89, 229, 252, 1438
HapII	CCGG	4 cut(s)	32, 52, 58, 244
HgaI	GACGC	1 cut(s)	139
HhaI	GCGC	5 cut(s)	412, 414, 985, 1109, 1416
Hin1II	CATG	7 cut(s)	566, 1025, 1072, 1298, 1350, 1420, 1484
Hin6I	GCGC	5 cut(s)	410, 412, 983, 1107, 1414
HinP1I	GCGC	5 cut(s)	410, 412, 983, 1107, 1414
HincII	GTYRAC	1 cut(s)	154
HindII	GTYRAC	1 cut(s)	154
HinfI	GANTC	4 cut(s)	280, 468, 1015, 1084
HpaII	CCGG	4 cut(s)	32, 52, 58, 244
HphI	GGTGA	3 cut(s)	408, 819, 926
Hpy166II	GTNNAC	3 cut(s)	154, 463, 841
Hpy188I	TCNGA	5 cut(s)	304, 327, 473, 1342, 1369
Hpy188III	TCNNGA	6 cut(s)	521, 593, 617, 672, 710, 1166
Hpy8I	GTNNAC	3 cut(s)	154, 463, 841
Hpy99I	CGWCG	3 cut(s)	16, 155, 158
HpyAV	CCTTC	5 cut(s)	37, 106, 828, 1225, 1237
HpyCH4III	ACNGT	8 cut(s)	167, 518, 560, 691, 862, 1004, 1354, 1376
HpyCH4IV	ACGT	2 cut(s)	364, 946
HpyCH4V	TGCA	9 cut(s)	344, 626, 641, 746, 944, 1196, 1298, 1337, 1405
HpyF3I	CTNAG	4 cut(s)	117, 303, 393, 492
HpySE526I	ACGT	2 cut(s)	364, 946
Hsp92II	CATG	7 cut(s)	566, 1025, 1072, 1298, 1350, 1420, 1484
HspAI	GCGC	5 cut(s)	410, 412, 983, 1107, 1414
Kzo9I	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
LguI	GCTCTTC	1 cut(s)	1452
LmnI	GCTCC	2 cut(s)	382, 1246
LweI	GCATC	5 cut(s)	734, 910, 931, 979, 1346
MaeI	CTAG	2 cut(s)	888, 957
MaeII	ACGT	2 cut(s)	364, 946
MaeIII	GTNAC	2 cut(s)	914, 1476
MalI	GATC	7 cut(s)	738, 763, 772, 996, 1056, 1077, 1430
MboI	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
MboII	GAAGA	9 cut(s)	49, 52, 121, 416, 439, 878, 989, 1454, 1469
MflI	RGATCY	2 cut(s)	736, 761
MhlI	GDGCHC	5 cut(s)	254, 387, 669, 852, 1305
MluCI	AATT	4 cut(s)	346, 629, 774, 1121
MmeI	TCCRAC	1 cut(s)	674
Mph1103I	ATGCAT	1 cut(s)	1339
MseI	TTAA	2 cut(s)	780, 1211
MslI	CAYNNNNRTG	1 cut(s)	1332
MspA1I	CMGCKG	3 cut(s)	289, 377, 743
MspI	CCGG	4 cut(s)	32, 52, 58, 244
MspR9I	CCNGG	7 cut(s)	52, 53, 453, 759, 786, 845, 1278
Mva1269I	GAATGC	1 cut(s)	1339
MvaI	CCWGG	5 cut(s)	453, 759, 786, 845, 1278
MvnI	CGCG	1 cut(s)	412
NciI	CCSGG	2 cut(s)	52, 53
NcoI	CCATGG	1 cut(s)	1346
NdeII	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
NlaIII	CATG	7 cut(s)	566, 1025, 1072, 1298, 1350, 1420, 1484
NlaIV	GGNNCC	3 cut(s)	56, 251, 252
NmeAIII	GCCGAG	1 cut(s)	74
NmuCI	GTSAC	2 cut(s)	914, 1476
NsiI	ATGCAT	1 cut(s)	1339
NspI	RCATGY	3 cut(s)	1072, 1298, 1484
NspV	TTCGAA	1 cut(s)	101
PaeI	GCATGC	1 cut(s)	1298
PaeR7I	CTCGAG	1 cut(s)	260
PauI	GCGCGC	1 cut(s)	410
PceI	AGGCCT	1 cut(s)	1438
PciI	ACATGT	1 cut(s)	1480
PciSI	GCTCTTC	1 cut(s)	1452
PctI	GAATGC	1 cut(s)	1339
PfeI	GAWTC	4 cut(s)	280, 468, 1015, 1084
PflMI	CCANNNNNTGG	1 cut(s)	764
PfoI	TCCNGGA	1 cut(s)	757
PmaCI	CACGTG	1 cut(s)	947
PmlI	CACGTG	1 cut(s)	947
Ppu21I	YACGTR	1 cut(s)	947
PscI	ACATGT	1 cut(s)	1480
PsiI	TTATAA	1 cut(s)	1119
Psp6I	CCWGG	5 cut(s)	451, 757, 784, 843, 1276
PspCI	CACGTG	1 cut(s)	947
PspGI	CCWGG	5 cut(s)	451, 757, 784, 843, 1276
PspN4I	GGNNCC	3 cut(s)	56, 251, 252
PspOMI	GGGCCC	1 cut(s)	250
PspPI	GGNCC	7 cut(s)	55, 88, 228, 250, 251, 712, 815
PspXI	VCTCGAGB	1 cut(s)	260
PstI	CTGCAG	1 cut(s)	1198
PstNI	CAGNNNCTG	1 cut(s)	764
PsuI	RGATCY	2 cut(s)	736, 761
PteI	GCGCGC	1 cut(s)	410
PvuII	CAGCTG	1 cut(s)	743
RsaI	GTAC	2 cut(s)	164, 434
RsaNI	GTAC	2 cut(s)	163, 433
RseI	CAYNNNNRTG	1 cut(s)	1332
SalI	GTCGAC	1 cut(s)	152
SapI	GCTCTTC	1 cut(s)	1452
SaqAI	TTAA	2 cut(s)	780, 1211
Sau3AI	GATC	7 cut(s)	736, 761, 770, 994, 1054, 1075, 1428
Sau96I	GGNCC	7 cut(s)	55, 88, 228, 250, 251, 712, 815
ScrFI	CCNGG	7 cut(s)	52, 53, 453, 759, 786, 845, 1278
SduI	GDGCHC	5 cut(s)	254, 387, 669, 852, 1305
SfaNI	GCATC	5 cut(s)	734, 910, 931, 979, 1346
SfcI	CTRYAG	3 cut(s)	904, 1194, 1432
Sfr274I	CTCGAG	1 cut(s)	260
SfuI	TTCGAA	1 cut(s)	101
SgrDI	CGTCGACG	1 cut(s)	152
SinI	GGWCC	2 cut(s)	712, 815
SlaI	CTCGAG	1 cut(s)	260
SmaI	CCCGGG	1 cut(s)	53
SmiMI	CAYNNNNRTG	1 cut(s)	1332
SmlI	CTYRAG	2 cut(s)	260, 1463
SmoI	CTYRAG	2 cut(s)	260, 1463
SphI	GCATGC	1 cut(s)	1298
Sse9I	AATT	4 cut(s)	346, 629, 774, 1121
SseBI	AGGCCT	1 cut(s)	1438
SsiI	CCGC	5 cut(s)	254, 289, 375, 380, 1081
SspMI	CTAG	2 cut(s)	888, 957
StuI	AGGCCT	1 cut(s)	1438
StyD4I	CCNGG	7 cut(s)	50, 51, 451, 757, 784, 843, 1276
StyI	CCWWGG	3 cut(s)	72, 830, 1346
TaaI	ACNGT	8 cut(s)	167, 518, 560, 691, 862, 1004, 1354, 1376
TaiI	ACGT	2 cut(s)	367, 949
TaqI	TCGA	3 cut(s)	101, 153, 261
TasI	AATT	4 cut(s)	346, 629, 774, 1121
TatI	WGTACW	2 cut(s)	162, 432
TauI	GCSGC	2 cut(s)	292, 377
TfiI	GAWTC	4 cut(s)	280, 468, 1015, 1084
Tru1I	TTAA	2 cut(s)	780, 1211
Tru9I	TTAA	2 cut(s)	780, 1211
TscAI	CASTG	6 cut(s)	176, 325, 696, 867, 1007, 1163
TseFI	GTSAC	2 cut(s)	914, 1476
Tsp45I	GTSAC	2 cut(s)	914, 1476
TspDTI	ATGAA	4 cut(s)	122, 417, 813, 1316
TspMI	CCCGGG	1 cut(s)	51
TspRI	CASTG	6 cut(s)	176, 325, 696, 867, 1007, 1163
Van91I	CCANNNNNTGG	1 cut(s)	764
VpaK11BI	GGWCC	2 cut(s)	712, 815
XapI	RAATTY	2 cut(s)	629, 1121
XceI	RCATGY	3 cut(s)	1072, 1298, 1484
XhoI	CTCGAG	1 cut(s)	260
XmaI	CCCGGG	1 cut(s)	51
XmiI	GTMKAC	2 cut(s)	153, 840
XspI	CTAG	2 cut(s)	888, 957
Zsp2I	ATGCAT	1 cut(s)	1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.