pycom09g11960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006744
Physical Location & Seq
Forward (+)
10219715 .. 10221526
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11960.3

Sequence Viewer

Length: 1473 bp
ATGCCGAAAGACGAAGAGAAAGGGAGGTCAGTGAGGAAGGCGCTGAGGGAGATATCGAACAACCATGGAGGTGGCGGCGGCGGAGGAGGAGGAGGAGGAGGGAGGTTCTCTAAATCATTCGCCACCGCGAAGAAGAAAGAAAAGGAAGCACACAAGCTCAGGGTCCAAGAACATGATAAAGAAGAAAAGGACCACCAAAACGACGACGCTCTCGATCGCCTCCTGCTCGTTCAGTCCGATCTCTCTGGCCTAACGCACCAGATTGATGAATTGGTTGTGCAAGCATTTAAATGCCAAGGAAAAGCAACCAAAGAAGTCGAATCCTTCACGAATTTCTTGTCTGAGATGCTTTCGTCTTTGAAGCCATGGGTGCCCAGGTTTCAGAAGGCACTCTCCTATCCGGTGGAGTCTGCTGCAGACGAAGGAGAGAGTGTACAGATTGCGAGTCCACAAGACTCCTTAATCTCTCCTTCTCCCCTTGTGTCTTGGCGTGCAGACTGCACTATCGAAAGAGGGAGACATCTGTTTAAGCTTACCCCTCTTCCCATATCAATGTCTCTGTCATCCAGACCCCCAGTCCCTTCTAGAACATCAGTCTTTGAGAGAATGACTTCGTGTACTGTTGATAGGATACCGAAGTTTCATGCTACTTTCGGAGATGCAAATGATGATTCGCTTGAGCTTTTGGCAGCAAAGCCAACTCCAATTAAGCCTTCTGATTCAGTTGCTACTGAAGCAGGAAGCAATCTCAAGCCTGGGTTTGTTTCTCCTCCGCCAATGTTTCCGAAGCAAGGGGGATCCATGGTTGTGAATGTGATGACTCCATGCCTAAAAATGTCTCCTCCTAAATCATGTGTTCTGCTCGAACCCTTTTCGGAGTCCTCTCCAAAGGGTCATCATGGGGTTCATAAATCCACCCCGTTTCCTGTTGGATTTACATATTGCAGCAATTCCTCTGGCAGTGAAGCTTCTGATGATCTGGCTTTCAGGTATCCAGAGCTGTTGGCACTACAACAGCCGTTCAAATCAGAAATTAGAAAGAACCATGTTGACTCATCACCAGAATGGTGCATATCGCCCCCAAAAACTTGTGTTTTACTTGATCCGCATGATGAAAAGTCTCCGAACAATGCTGATACTGATTCCCAATTGCTCATTACCGGGCGTCATCTAGATAACCATATCAATTTTTCTGTATCAAAAGAAGATGATGGTCAAGGAGGCATTCACCCAATCAAGACATCTTGTCTTCATGAGCCTGCAGGTGGCAGCTTTGCAATGATCGAAAGCACTCCCATCTGGAAAGAAGCTGAAAGCGTTGTTCGAGGGGGCAAACTTCCTGGTGAGCATACTCTAAAGCGGGAGTTATGGACAAAGTTTGAAGCAGCTTCTATGAATGGGGTTCGATTTAACGACACTGCAACTCATAAAGGATTTCTTGACCGATTGGATGAGGTTGTCGACGGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

491

Amino Acids

53.49

Weight (kDa)

6.16

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1253
Acc36I ACCTGC 1 cut(s) 1253
AccB1I GGYRCC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 1461
AccII CGCG 1 cut(s) 128
AciI CCGC 7 cut(s) 75, 78, 81, 126, 773, 1106, 1360
AclWI GGATC 3 cut(s) 792, 805, 1097
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 753
AcyI GRCGYC 1 cut(s) 1165
AfaI GTAC 2 cut(s) 435, 619
AfiI CCNNNNNNNGG 2 cut(s) 791, 1265
AgsI TTSAA 3 cut(s) 361, 1024, 1382
AhdI GACNNNNNGTC 2 cut(s) 575, 1245
AjnI CCWGG 3 cut(s) 374, 754, 1339
AjuI GAANNNNNNNTTGG 2 cut(s) 697, 729
AluBI AGCT 8 cut(s) 157, 532, 682, 968, 1000, 1272, 1310, 1388
AluI AGCT 8 cut(s) 157, 532, 682, 968, 1000, 1272, 1310, 1388
Alw26I GTCTC 4 cut(s) 511, 561, 843, 1125
AlwI GGATC 3 cut(s) 792, 805, 1097
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 5 cut(s) 413, 689, 945, 1269, 1385
ApoI RAATTY 1 cut(s) 331
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
Asp700I GAANNNNTTC 1 cut(s) 610
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 163, 190
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 3 cut(s) 1050, 1220, 1355
AvaII GGWCC 2 cut(s) 163, 190
BaeGI GKGCMC 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 623, 656
BamHI GGATCC 1 cut(s) 797
BanI GGYRCC 1 cut(s) 370
BbsI GAAGAC 1 cut(s) 1241
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 5 cut(s) 400, 701, 957, 1281, 1397
BccI CCATC 2 cut(s) 1205, 1304
BceAI ACGGC 1 cut(s) 1003
BciT130I CCWGG 3 cut(s) 376, 756, 1341
BciVI GTATCC 2 cut(s) 624, 1002
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 4 cut(s) 511, 561, 843, 1125
BfaI CTAG 2 cut(s) 585, 1172
BfmI CTRYAG 2 cut(s) 414, 1260
BfoI RGCGCY 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 1253
BfuI GTATCC 2 cut(s) 624, 1002
BisI GCNGC 7 cut(s) 76, 79, 414, 690, 946, 1270, 1386
BlsI GCNGC 7 cut(s) 77, 80, 415, 691, 947, 1271, 1387
Bme1390I CCNGG 4 cut(s) 376, 756, 1162, 1341
Bme18I GGWCC 2 cut(s) 163, 190
BmeRI GACNNNNNGTC 2 cut(s) 575, 1245
BmgT120I GGNCC 2 cut(s) 163, 190
BmiI GGNNCC 3 cut(s) 164, 372, 799
BmrFI CCNGG 4 cut(s) 376, 756, 1162, 1341
BmrI ACTGGG 1 cut(s) 569
BmsI GCATC 2 cut(s) 336, 649
BmuI ACTGGG 1 cut(s) 569
BpiI GAAGAC 1 cut(s) 1241
Bpu10I CCTNAGC 2 cut(s) 44, 158
BpuEI CTTGAG 2 cut(s) 698, 734
BpuMI CCSGG 1 cut(s) 1162
BsaHI GRCGYC 1 cut(s) 1165
BsaJI CCNNGG 6 cut(s) 64, 295, 365, 374, 755, 801
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 791, 1265
Bse1I ACTGG 1 cut(s) 575
Bse3DI GCAATG 1 cut(s) 1284
BseBI CCWGG 3 cut(s) 376, 756, 1341
BseDI CCNNGG 6 cut(s) 64, 295, 365, 374, 755, 801
BseGI GGATG 2 cut(s) 563, 1456
BseLI CCNNNNNNNGG 2 cut(s) 791, 1265
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 3 cut(s) 35, 172, 333
BseNI ACTGG 1 cut(s) 575
BseRI GAGGAG 7 cut(s) 99, 102, 105, 108, 111, 759, 831
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 5 cut(s) 400, 701, 957, 1281, 1397
BsgI GTGCAG 2 cut(s) 484, 513
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 217
BsiSI CCGG 2 cut(s) 401, 1161
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 2 cut(s) 791, 1265
BsmAI GTCTC 4 cut(s) 511, 561, 843, 1125
BsmFI GGGAC 1 cut(s) 563
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 375
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
Bsp19I CCATGG 3 cut(s) 64, 365, 801
BspACI CCGC 7 cut(s) 75, 78, 81, 126, 773, 1106, 1360
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 3 cut(s) 36, 171, 334
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 3 cut(s) 164, 372, 799
BspMAI CTGCAG 2 cut(s) 418, 1264
BspMI ACCTGC 1 cut(s) 1253
BspPI GGATC 3 cut(s) 792, 805, 1097
BspT107I GGYRCC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 1284
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 1 cut(s) 575
BssECI CCNNGG 6 cut(s) 64, 295, 365, 374, 755, 801
BssMI GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
BssNI GRCGYC 1 cut(s) 1165
BssT1I CCWWGG 4 cut(s) 64, 295, 365, 801
Bst2UI CCWGG 3 cut(s) 376, 756, 1341
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 2 cut(s) 9, 546
BstACI GRCGYC 1 cut(s) 1165
BstAUI TGTACA 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 282, 492, 1260
BstDEI CTNAG 3 cut(s) 44, 158, 342
BstDSI CCRYGG 3 cut(s) 64, 365, 801
BstF5I GGATG 2 cut(s) 563, 1456
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 6 cut(s) 217, 241, 800, 979, 1105, 1284
BstMAI GTCTC 4 cut(s) 511, 561, 843, 1125
BstMBI GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 2 cut(s) 370, 734
BstNI CCWGG 3 cut(s) 376, 756, 1341
BstSCI CCNGG 4 cut(s) 374, 754, 1160, 1339
BstSFI CTRYAG 2 cut(s) 414, 1260
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 5 cut(s) 400, 701, 957, 1281, 1397
BstV2I GAAGAC 1 cut(s) 1241
BstX2I RGATCY 1 cut(s) 797
BstXI CCANNNNNNTGG 1 cut(s) 71
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 2 cut(s) 624, 1002
BsuRI GGCC 1 cut(s) 249
BtgI CCRYGG 3 cut(s) 64, 365, 801
BtsCI GGATG 2 cut(s) 563, 1456
BtsI GCAGTG 2 cut(s) 967, 1416
BtsIMutI CAGTG 3 cut(s) 36, 967, 1416
BveI ACCTGC 1 cut(s) 1253
Cac8I GCNNGC 3 cut(s) 282, 492, 1260
CciI TCATGA 1 cut(s) 1252
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 163, 190
CseI GACGC 2 cut(s) 215, 1154
Csp6I GTAC 2 cut(s) 434, 618
CviQI GTAC 2 cut(s) 434, 618
DdeI CTNAG 3 cut(s) 44, 158, 342
DpnI GATC 6 cut(s) 216, 240, 799, 978, 1104, 1283
DpnII GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
DraI TTTAAA 1 cut(s) 289
DriI GACNNNNNGTC 2 cut(s) 575, 1245
Eam1104I CTCTTC 2 cut(s) 9, 546
Eam1105I GACNNNNNGTC 2 cut(s) 575, 1245
EarI CTCTTC 2 cut(s) 9, 546
EciI GGCGGA 2 cut(s) 96, 762
Eco130I CCWWGG 4 cut(s) 64, 295, 365, 801
Eco32I GATATC 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 163, 190
Eco57I CTGAAG 1 cut(s) 753
EcoRII CCWGG 3 cut(s) 374, 754, 1339
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 4 cut(s) 64, 295, 365, 801
ErhI CCWWGG 4 cut(s) 64, 295, 365, 801
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 1 cut(s) 563
FauI CCCGC 1 cut(s) 1353
FblI GTMKAC 1 cut(s) 1461
Fnu4HI GCNGC 7 cut(s) 76, 79, 414, 690, 946, 1270, 1386
FokI GGATG 2 cut(s) 550, 1463
Fsp4HI GCNGC 7 cut(s) 76, 79, 414, 690, 946, 1270, 1386
FspBI CTAG 2 cut(s) 585, 1172
GlaI GCGC 1 cut(s) 42
GluI GCNGC 7 cut(s) 76, 79, 414, 690, 946, 1270, 1386
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 1 cut(s) 249
HapII CCGG 2 cut(s) 401, 1161
HgaI GACGC 2 cut(s) 215, 1154
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 1165
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 2 cut(s) 1051, 1462
HindII GTYRAC 2 cut(s) 1051, 1462
HindIII AAGCTT 2 cut(s) 530, 966
HpaII CCGG 2 cut(s) 401, 1161
HphI GGTGA 3 cut(s) 1050, 1220, 1355
Hpy166II GTNNAC 5 cut(s) 434, 449, 618, 1051, 1462
Hpy8I GTNNAC 5 cut(s) 434, 449, 618, 1051, 1462
Hpy99I CGWCG 3 cut(s) 206, 209, 1466
HpyAV CCTTC 7 cut(s) 31, 334, 379, 416, 480, 591, 723
HpyCH4III ACNGT 1 cut(s) 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 734
HpyF3I CTNAG 3 cut(s) 44, 158, 342
Hsp92I GRCGYC 1 cut(s) 1165
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
Lsp1109I GCAGC 5 cut(s) 400, 701, 957, 1281, 1397
LweI GCATC 2 cut(s) 336, 649
MaeI CTAG 2 cut(s) 585, 1172
MalI GATC 6 cut(s) 216, 240, 799, 978, 1104, 1283
MboI GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
MboII GAAGA 7 cut(s) 26, 142, 145, 194, 533, 1217, 1241
MfeI CAATTG 1 cut(s) 1148
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 7 cut(s) 269, 331, 705, 949, 1032, 1148, 1186
MlyI GAGTC 6 cut(s) 416, 449, 454, 814, 887, 1046
MmeI TCCRAC 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 5 cut(s) 288, 461, 528, 708, 1410
MslI CAYNNNNRTG 5 cut(s) 69, 289, 551, 806, 1063
MspI CCGG 2 cut(s) 401, 1161
MspR9I CCNGG 4 cut(s) 376, 756, 1162, 1341
MunI CAATTG 1 cut(s) 1148
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 3 cut(s) 376, 756, 1341
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 2 cut(s) 370, 734
NciI CCSGG 1 cut(s) 1162
NcoI CCATGG 3 cut(s) 64, 365, 801
NdeII GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
NlaIV GGNNCC 3 cut(s) 164, 372, 799
PagI TCATGA 1 cut(s) 1252
PaqCI CACCTGC 1 cut(s) 1253
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 234
PctI GAATGC 1 cut(s) 1224
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 4 cut(s) 320, 671, 719, 1142
PkrI GCNGC 7 cut(s) 77, 80, 415, 691, 947, 1271, 1387
Ple19I CGATCG 1 cut(s) 217
PleI GAGTC 6 cut(s) 415, 449, 453, 814, 886, 1046
PpsI GAGTC 6 cut(s) 415, 449, 453, 814, 886, 1046
Psp6I CCWGG 3 cut(s) 374, 754, 1339
PspGI CCWGG 3 cut(s) 374, 754, 1339
PspN4I GGNNCC 3 cut(s) 164, 372, 799
PspPI GGNCC 2 cut(s) 163, 190
PstI CTGCAG 2 cut(s) 418, 1264
PsuI RGATCY 1 cut(s) 797
PvuI CGATCG 1 cut(s) 217
RsaI GTAC 2 cut(s) 435, 619
RsaNI GTAC 2 cut(s) 434, 618
RseI CAYNNNNRTG 5 cut(s) 69, 289, 551, 806, 1063
SalI GTCGAC 1 cut(s) 1460
SaqAI TTAA 5 cut(s) 288, 461, 528, 708, 1410
SatI GCNGC 7 cut(s) 76, 79, 414, 690, 946, 1270, 1386
Sau3AI GATC 6 cut(s) 214, 238, 797, 976, 1102, 1281
Sau96I GGNCC 2 cut(s) 163, 190
SbfI CCTGCAGG 1 cut(s) 1264
SchI GAGTC 6 cut(s) 416, 449, 454, 814, 887, 1046
ScrFI CCNGG 4 cut(s) 376, 756, 1162, 1341
SdaI CCTGCAGG 1 cut(s) 1264
SduI GDGCHC 1 cut(s) 375
SfaNI GCATC 2 cut(s) 336, 649
SfcI CTRYAG 2 cut(s) 414, 1260
SinI GGWCC 2 cut(s) 163, 190
SmiI ATTTAAAT 1 cut(s) 289
SmiMI CAYNNNNRTG 5 cut(s) 69, 289, 551, 806, 1063
SmlI CTYRAG 2 cut(s) 677, 749
SmoI CTYRAG 2 cut(s) 677, 749
Sse8387I CCTGCAGG 1 cut(s) 1264
Sse9I AATT 7 cut(s) 269, 331, 705, 949, 1032, 1148, 1186
SsiI CCGC 7 cut(s) 75, 78, 81, 126, 773, 1106, 1360
SspMI CTAG 2 cut(s) 585, 1172
StyD4I CCNGG 4 cut(s) 374, 754, 1160, 1339
StyI CCWWGG 4 cut(s) 64, 295, 365, 801
SwaI ATTTAAAT 1 cut(s) 289
TaaI ACNGT 1 cut(s) 622
TaqI TCGA 9 cut(s) 56, 213, 318, 507, 864, 1284, 1324, 1405, 1461
TaqII GACCGA 1 cut(s) 1458
TasI AATT 7 cut(s) 269, 331, 705, 949, 1032, 1148, 1186
TatI WGTACW 2 cut(s) 433, 617
TauI GCSGC 2 cut(s) 78, 81
TfiI GAWTC 4 cut(s) 320, 671, 719, 1142
Tru1I TTAA 5 cut(s) 288, 461, 528, 708, 1410
Tru9I TTAA 5 cut(s) 288, 461, 528, 708, 1410
TscAI CASTG 3 cut(s) 36, 967, 1423
TseI GCWGC 5 cut(s) 413, 689, 945, 1269, 1385
TspDTI ATGAA 6 cut(s) 282, 632, 896, 1128, 1241, 1409
TspRI CASTG 3 cut(s) 36, 967, 1423
VpaK11BI GGWCC 2 cut(s) 163, 190
XapI RAATTY 1 cut(s) 331
XbaI TCTAGA 2 cut(s) 584, 1171
XmiI GTMKAC 1 cut(s) 1461
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 2 cut(s) 585, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.