pycom09g12010

malic enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006459
Physical Location & Seq
Reverse (-)
10305506 .. 10307872
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGACTGCAGTCAAGCAGAATTACGGGGAGAGAATTCTTGTTCAGTTTGAAGACTTTGCAAACCACAATGCTTTTGATCTGCTCGCGAAGTATGCCTCAACCCATCTTGTTTTTAATGATGACATTCAGGGGACAGCATCGGTGGTTCTTGCAGGGCTTCTTGCAGCTTTAAAGTTAGTAAAGGGATCCTTATCTGAACACAGATTTTTATTCCTTGGTGCTGGAGAGGCCGGCACTGGCATAGCAGAACTCATAGCCCTTGAAATTTCCAAGCAGACAAATACCCCAGTGGAAGAGACCCGCAAGAATATTTGGCTTGTGGACTCAAAGGGATTGATTGTCAGTTCTCGGTTGGATTCACTCCAACACTTTAAAAAGCCCTGGGCTCATGAACATGAACCAGTCGGGGAACTTGTAGATGCTGTTAAGTCAATCAAGCCAACAGTATTAATTGGAACGTCAGGCGTTGGAAGAACATTCACTAAAGAAGTGGTTGAGGCTATGGCTTCCCTGAATGAGAGACCTATTATTCTCGCTCTTTCCAATCCAACATCACAATCTGAATGTACCGCGGAAGAAGCATATACATGGACTGAGGGTCGTGCCATATATTGTAGTGGAAGCCCATTCCCGCCAGTTGAATATAATGGGAAGGTTTACTATCCTGGCCAGGCAAACAATGCATACATCTTCCCTGGATTCGGTCTGGGTTTAATAATGTCTGGTACTATCCGTGTTCATGACGACATGCTTCTGGCAGCTTCGGAAGCGTTGGCTTCAATGGTGAGCCAGGAAGACTTTGACAAGGGACTCATATACCCTCCATTCACAAACATCAGAAAGATTTCGGCACATATTGCTGCTAAAGTAGCTGCAAAATCATATGAACTTGGTTTGGCCACCCGCCTCCCCGAACCAAGAGATCTAGAGAAGTATGCTGAAAGCTGTATGTACAGCCCTTCCTATCGAAATTTCCGGTAA

Protein Analysis

327

Amino Acids

35.74

Weight (kDa)

5.81

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malic_M PF03949 42 - 293 1.1e-98 Malic enzyme, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 86, 572
AciI CCGC 5 cut(s) 301, 570, 572, 632, 904
AclWI GGATC 2 cut(s) 180, 193
AcoI YGGCCR 2 cut(s) 667, 897
AcsI RAATTY 3 cut(s) 33, 264, 970
AfaI GTAC 3 cut(s) 568, 727, 953
AfiI CCNNNNNNNGG 1 cut(s) 701
AgsI TTSAA 4 cut(s) 50, 263, 641, 780
AhdI GACNNNNNGTC 1 cut(s) 597
AjnI CCWGG 5 cut(s) 380, 664, 669, 694, 789
AluBI AGCT 4 cut(s) 167, 761, 872, 945
AluI AGCT 4 cut(s) 167, 761, 872, 945
Alw26I GTCTC 2 cut(s) 290, 514
AlwI GGATC 2 cut(s) 180, 193
AoxI GGCC 3 cut(s) 228, 667, 897
ApeKI GCWGC 4 cut(s) 164, 758, 860, 872
ApoI RAATTY 3 cut(s) 33, 264, 970
AseI ATTAAT 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 844
AsuHPI GGTGA 1 cut(s) 796
BalI TGGCCA 2 cut(s) 669, 899
BamHI GGATCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 57, 801
BbvI GCAGC 4 cut(s) 176, 770, 847, 859
BccI CCATC 1 cut(s) 111
BciT130I CCWGG 5 cut(s) 382, 666, 671, 696, 791
BcoDI GTCTC 2 cut(s) 290, 514
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 922
BisI GCNGC 4 cut(s) 165, 759, 861, 873
BlsI GCNGC 4 cut(s) 166, 760, 862, 874
Bme1390I CCNGG 5 cut(s) 382, 666, 671, 696, 791
BmeRI GACNNNNNGTC 1 cut(s) 597
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 5 cut(s) 382, 666, 671, 696, 791
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 2 cut(s) 146, 409
BmuI ACTGGG 1 cut(s) 281
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 57, 801
BpmI CTGGAG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 190
BsaI GGTCTC 2 cut(s) 290, 514
BsaJI CCNNGG 5 cut(s) 214, 380, 381, 570, 694
BsaWI WCCGGW 1 cut(s) 975
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 4 cut(s) 241, 287, 401, 635
Bse8I GATNNNNATC 1 cut(s) 190
BseBI CCWGG 5 cut(s) 382, 666, 671, 696, 791
BseDI CCNNGG 5 cut(s) 214, 380, 381, 570, 694
BseJI GATNNNNATC 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 4 cut(s) 241, 287, 401, 635
BseXI GCAGC 4 cut(s) 176, 770, 847, 859
Bsh1236I CGCG 2 cut(s) 86, 572
BshFI GGCC 3 cut(s) 230, 669, 899
BsiSI CCGG 2 cut(s) 231, 976
BslFI GGGAC 2 cut(s) 145, 822
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 2 cut(s) 290, 514
BsmFI GGGAC 2 cut(s) 145, 822
BsnI GGCC 3 cut(s) 230, 669, 899
Bso31I GGTCTC 2 cut(s) 290, 514
Bsp1286I GDGCHC 1 cut(s) 388
Bsp1407I TGTACA 1 cut(s) 951
Bsp143I GATC 3 cut(s) 76, 185, 922
Bsp68I TCGCGA 1 cut(s) 86
BspACI CCGC 5 cut(s) 301, 570, 572, 632, 904
BspANI GGCC 3 cut(s) 230, 669, 899
BspCNI CTCAG 1 cut(s) 586
BspFNI CGCG 2 cut(s) 86, 572
BspHI TCATGA 2 cut(s) 388, 739
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 180, 193
BspTNI GGTCTC 2 cut(s) 290, 514
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 951
BsrI ACTGG 4 cut(s) 241, 287, 401, 635
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 5 cut(s) 214, 380, 381, 570, 694
BssMI GATC 3 cut(s) 76, 185, 922
BssT1I CCWWGG 1 cut(s) 214
Bst2UI CCWGG 5 cut(s) 382, 666, 671, 696, 791
Bst4CI ACNGT 1 cut(s) 445
Bst6I CTCTTC 1 cut(s) 288
BstAPI GCANNNNNTGC 2 cut(s) 680, 857
BstAUI TGTACA 1 cut(s) 951
BstC8I GCNNGC 2 cut(s) 84, 232
BstDEI CTNAG 1 cut(s) 594
BstDSI CCRYGG 1 cut(s) 570
BstFNI CGCG 2 cut(s) 86, 572
BstKTI GATC 3 cut(s) 79, 188, 925
BstMAI GTCTC 2 cut(s) 290, 514
BstMBI GATC 3 cut(s) 76, 185, 922
BstMWI GCNNNNNNNGC 7 cut(s) 92, 227, 578, 680, 767, 857, 869
BstNI CCWGG 5 cut(s) 382, 666, 671, 696, 791
BstNSI RCATGY 1 cut(s) 751
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 5 cut(s) 380, 664, 669, 694, 789
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 2 cut(s) 86, 572
BstV1I GCAGC 4 cut(s) 176, 770, 847, 859
BstV2I GAAGAC 2 cut(s) 57, 801
BstX2I RGATCY 2 cut(s) 185, 922
BstYI RGATCY 2 cut(s) 185, 922
BsuRI GGCC 3 cut(s) 230, 669, 899
BtgI CCRYGG 1 cut(s) 570
BtsIMutI CAGTG 2 cut(s) 234, 294
BtuMI TCGCGA 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 84, 232
CciI TCATGA 2 cut(s) 388, 739
Cfr10I RCCGGY 1 cut(s) 230
Cfr42I CCGCGG 1 cut(s) 573
Csp6I GTAC 3 cut(s) 567, 726, 952
CviAII CATG 5 cut(s) 389, 395, 588, 740, 748
CviQI GTAC 3 cut(s) 567, 726, 952
DdeI CTNAG 1 cut(s) 594
DpnI GATC 3 cut(s) 78, 187, 924
DpnII GATC 3 cut(s) 76, 185, 922
DraI TTTAAA 2 cut(s) 171, 373
DriI GACNNNNNGTC 1 cut(s) 597
EaeI YGGCCR 2 cut(s) 667, 897
Eam1104I CTCTTC 1 cut(s) 288
Eam1105I GACNNNNNGTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 2 cut(s) 290, 514
EcoRI GAATTC 1 cut(s) 33
EcoRII CCWGG 5 cut(s) 380, 664, 669, 694, 789
EcoT14I CCWWGG 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 5 cut(s) 392, 398, 591, 743, 751
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 145, 822
FatI CATG 5 cut(s) 388, 394, 587, 739, 747
FauI CCCGC 3 cut(s) 308, 639, 911
FauNDI CATATG 1 cut(s) 883
Fnu4HI GCNGC 4 cut(s) 165, 759, 861, 873
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 4 cut(s) 165, 759, 861, 873
FspBI CTAG 1 cut(s) 926
GluI GCNGC 4 cut(s) 165, 759, 861, 873
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 3 cut(s) 230, 669, 899
HapII CCGG 2 cut(s) 231, 976
Hin1II CATG 5 cut(s) 392, 398, 591, 743, 751
HinfI GANTC 4 cut(s) 323, 356, 699, 810
HpaII CCGG 2 cut(s) 231, 976
HphI GGTGA 1 cut(s) 796
Hpy166II GTNNAC 2 cut(s) 322, 658
Hpy188I TCNGA 4 cut(s) 196, 562, 766, 839
Hpy188III TCNNGA 4 cut(s) 85, 389, 740, 926
Hpy8I GTNNAC 2 cut(s) 322, 658
HpyAV CCTTC 2 cut(s) 646, 969
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 1 cut(s) 458
HpyCH4V TGCA 6 cut(s) 8, 59, 152, 164, 683, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 227, 578, 680, 767, 857, 869
HpyF3I CTNAG 1 cut(s) 594
HpySE526I ACGT 1 cut(s) 458
Hsp92II CATG 5 cut(s) 392, 398, 591, 743, 751
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 573
Kzo9I GATC 3 cut(s) 76, 185, 922
Lsp1109I GCAGC 4 cut(s) 176, 770, 847, 859
LweI GCATC 2 cut(s) 146, 409
MaeI CTAG 1 cut(s) 926
MaeII ACGT 1 cut(s) 458
MalI GATC 3 cut(s) 78, 187, 924
MboI GATC 3 cut(s) 76, 185, 922
MboII GAAGA 6 cut(s) 62, 305, 483, 587, 682, 806
MflI RGATCY 2 cut(s) 185, 922
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 2 cut(s) 669, 899
MluCI AATT 5 cut(s) 19, 33, 264, 450, 970
MluNI TGGCCA 2 cut(s) 669, 899
MlyI GAGTC 2 cut(s) 317, 804
MmeI TCCRAC 4 cut(s) 333, 388, 448, 572
MnlI CCTC 6 cut(s) 106, 220, 490, 589, 831, 917
Mox20I TGGCCA 2 cut(s) 669, 899
Mph1103I ATGCAT 1 cut(s) 685
MroNI GCCGGC 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 844
MscI TGGCCA 2 cut(s) 669, 899
MseI TTAA 6 cut(s) 114, 170, 372, 426, 449, 713
MslI CAYNNNNRTG 2 cut(s) 393, 586
Msp20I TGGCCA 2 cut(s) 669, 899
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 2 cut(s) 231, 976
MspR9I CCNGG 5 cut(s) 382, 666, 671, 696, 791
MvaI CCWGG 5 cut(s) 382, 666, 671, 696, 791
MvnI CGCG 2 cut(s) 86, 572
MwoI GCNNNNNNNGC 7 cut(s) 92, 227, 578, 680, 767, 857, 869
NaeI GCCGGC 1 cut(s) 232
NdeI CATATG 1 cut(s) 883
NdeII GATC 3 cut(s) 76, 185, 922
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 5 cut(s) 392, 398, 591, 743, 751
NlaIV GGNNCC 1 cut(s) 187
NruI TCGCGA 1 cut(s) 86
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 751
PagI TCATGA 2 cut(s) 388, 739
PasI CCCWGGG 1 cut(s) 381
PdiI GCCGGC 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 2 cut(s) 356, 699
PkrI GCNGC 4 cut(s) 166, 760, 862, 874
PleI GAGTC 2 cut(s) 317, 804
PpsI GAGTC 2 cut(s) 317, 804
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 449
Psp6I CCWGG 5 cut(s) 380, 664, 669, 694, 789
PspGI CCWGG 5 cut(s) 380, 664, 669, 694, 789
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 185, 922
RruI TCGCGA 1 cut(s) 86
RsaI GTAC 3 cut(s) 568, 727, 953
RsaNI GTAC 3 cut(s) 567, 726, 952
RseI CAYNNNNRTG 2 cut(s) 393, 586
SacII CCGCGG 1 cut(s) 573
SaqAI TTAA 6 cut(s) 114, 170, 372, 426, 449, 713
SatI GCNGC 4 cut(s) 165, 759, 861, 873
Sau3AI GATC 3 cut(s) 76, 185, 922
SchI GAGTC 2 cut(s) 317, 804
ScrFI CCNGG 5 cut(s) 382, 666, 671, 696, 791
SduI GDGCHC 1 cut(s) 388
SetI ASST 7 cut(s) 169, 461, 526, 657, 763, 874, 947
SfaNI GCATC 2 cut(s) 146, 409
SfcI CTRYAG 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 573
SgrBI CCGCGG 1 cut(s) 573
SmiMI CAYNNNNRTG 2 cut(s) 393, 586
Sse9I AATT 5 cut(s) 19, 33, 264, 450, 970
SsiI CCGC 5 cut(s) 301, 570, 572, 632, 904
SspI AATATT 1 cut(s) 310
SspMI CTAG 1 cut(s) 926
StyD4I CCNGG 5 cut(s) 380, 664, 669, 694, 789
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 1 cut(s) 461
TaqI TCGA 1 cut(s) 967
TaqII GACCGA 1 cut(s) 692
TasI AATT 5 cut(s) 19, 33, 264, 450, 970
TatI WGTACW 1 cut(s) 951
TfiI GAWTC 2 cut(s) 356, 699
Tru1I TTAA 6 cut(s) 114, 170, 372, 426, 449, 713
Tru9I TTAA 6 cut(s) 114, 170, 372, 426, 449, 713
TscAI CASTG 2 cut(s) 241, 294
TseI GCWGC 4 cut(s) 164, 758, 860, 872
TspDTI ATGAA 4 cut(s) 405, 411, 728, 900
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 2 cut(s) 241, 294
VspI ATTAAT 1 cut(s) 449
XapI RAATTY 3 cut(s) 33, 264, 970
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 1 cut(s) 926
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.