Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0013015

Physical Location & Seq

Genomic Coordinates Reverse (-)

17205337 .. 17206656

Gene Structure 1320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g16830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGAAACAAGCGGAGAATGGGAGGCGAACAAAGACAAGGAACTTGATTTTTCCTTCGAGAGGCCGCCATGGAAGCCCGGTTTTAGTGAGGCTTCAACGGCGTCGAATAGGCCTCTCAAGAAAGTCAAGAGCCCTGAAAGGCAAGACCCAATTCAAGCTTCACCTTCTTTAGCTCATCATCAAACACCTTCGCCAATCATCGCTCCCCATCCATCTTCTTCCTCTTCAAAAATAGTTTTTCCTTTTTCTTTCGACGGATCTCAACAGCCTATGATGCAATTCCCCCACCCGTTCAACCCCACAAATTTGCCTATGTTTCCCTCACCAATTCAACAGCAACAGCAACAAAATCCGCAGCAAATGATTTCTTTTGAATCCCACCAGCAGCAGCAGCAGGGGATGAGTTATCCATCGCCTTTTGTCGGAGAGTCGGTGAGTTGGCAGCAGCACCAGAAAATGCTTCAGCATTGGAGTGACGCTTTGAATTTGAGTCCAAGAGGGAGGGTGATGATGATGAACAGGTTGGGACCAGATGGCAGGCCAATGTTACGACCCCCGGCAATGCCTTATAATACTACAAAACTTTACAGGGGAGTGAGGCAACGGCATTGGGGCAAATGGGTAGCTGAAATCCGCCTTCCTCGAAACAGGACTCGCCTCTGGCTTGGCACCTTTGACACAGCTGAAGATGCCGCCATGGCTTACGACCGCGAGGCCTTCAAGTTGAGAGGAGAGAATGCCAGGCTCAATTTCCCTGAGCTTTTTCTCAATAAAGACAAATCAGATGTTTCAGTTTCATCAGCTCCAAGTTCAGCCACTATTTCGCCTCCAAGGCATGAAGGTTCAAAACCAACCAGGCACCGCAAGCAACCTCAAAAAATCAAGGGAAGCGCTTCGAATATAGAGGCAATGCCGCCGCCACCAATTCCACACACCCAAGAAGAGAATAGTACTGACAATGATTCGGGACTGGAGTCAAGCGAGGCTAAAGCGATTGACGAAGTTGAGGCGAATACGGATGGTGCTGGAGGAGGGAGTTCACAGCAAAAGGAAATTGTTTGGGGGGATATGGCAGAAGATTGGCTTAATGCAATTCCTGCAGGTTGGGGTCCAGGTAGTCCCGTGTGGGATGATTTGGACACCAACAACAGTCTTTTGCTGCAATCAGATCTCCCTTTTACCAATCCAAATCAGCAGGACTATAACATTAACATTAATCCTCAACAAGCGATTCAGAATTCAGGTTCGGGTTCGGGATCTGGTTCCTCTTCTTCCTCTTCATATCCCATGAAGAACCTCTTTTGGAAGGACCAGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0009651 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0080135 GO:0097159 GO:0140110 GO:1900034 GO:1900036 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000070 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

440

Amino Acids

49.04

Weight (kDa)

6.03

Isoelectric Point (pI)

62.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AP2	PF00847	195 - 244	1.7e-13	AP2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013015)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20880 AT4G28140
fragaria_vesca	FvH4_6g26090
malus_domestica	MD09G1252000.v1.1 MD17G1244300.v1.1
prunus_persica	Prupe.3G157100_v2.0.a1
pyrus_communis	pycom09g16830 pycom17g24710
rosa_chinensis	RchiOBHm_Chr2g0126301
rosa_laevigata	RLG00000018898
rosa_multiflora	Rmu_ssc0000264.1_g000034
rosa_roxburghii	Rroxscaffold_2G00117280
rosa_rugosa	Rorug02G0262400
rosa_samantha	Rh2AG319300 Rh2BG327700 Rh2CG307700 Rh2DG347300
rosa_wichuraiana	Rw2G025870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	570
Acc36I	ACCTGC	1 cut(s)	1091
AccB1I	GGYRCC	2 cut(s)	668, 858
AccII	CGCG	1 cut(s)	711
AciI	CCGC	9 cut(s)	12, 65, 353, 634, 693, 709, 862, 914, 917
AclWI	GGATC	2 cut(s)	265, 1264
AcsI	RAATTY	3 cut(s)	304, 484, 1237
AcuI	CTGAAG	2 cut(s)	446, 705
AcyI	GRCGYC	1 cut(s)	101
AfaI	GTAC	1 cut(s)	952
AfeI	AGCGCT	1 cut(s)	892
AfiI	CCNNNNNNNGG	2 cut(s)	60, 422
AgsI	TTSAA	9 cut(s)	96, 155, 228, 295, 332, 374, 484, 721, 846
AjnI	CCWGG	4 cut(s)	740, 854, 1111, 1311
AjuI	GAANNNNNNNTTGG	2 cut(s)	142, 174
AluBI	AGCT	6 cut(s)	158, 173, 626, 683, 760, 803
AluI	AGCT	6 cut(s)	158, 173, 626, 683, 760, 803
AlwI	GGATC	2 cut(s)	265, 1264
Aor51HI	AGCGCT	1 cut(s)	892
AoxI	GGCC	4 cut(s)	62, 110, 539, 714
ApeKI	GCWGC	7 cut(s)	355, 385, 388, 391, 442, 445, 1159
ApoI	RAATTY	3 cut(s)	304, 484, 1237
AseI	ATTAAT	1 cut(s)	1215
Asp700I	GAANNNNTTC	1 cut(s)	892
AspLEI	GCGC	1 cut(s)	893
AspS9I	GGNCC	3 cut(s)	527, 1109, 1309
AsuC2I	CCSGG	2 cut(s)	78, 557
AsuHPI	GGTGA	4 cut(s)	153, 315, 445, 517
AsuII	TTCGAA	1 cut(s)	896
AvaII	GGWCC	3 cut(s)	527, 1109, 1309
BanI	GGYRCC	2 cut(s)	668, 858
BanII	GRGCYC	1 cut(s)	134
BbvI	GCAGC	7 cut(s)	367, 397, 400, 403, 454, 457, 1146
BccI	CCATC	5 cut(s)	216, 220, 418, 527, 1013
BceAI	ACGGC	2 cut(s)	114, 620
BciT130I	CCWGG	4 cut(s)	742, 856, 1113, 1313
BcnI	CCSGG	2 cut(s)	78, 557
BfmI	CTRYAG	1 cut(s)	1098
BfoI	RGCGCY	1 cut(s)	894
BfuAI	ACCTGC	1 cut(s)	1091
BglI	GCCNNNNNGGC	2 cut(s)	698, 832
BglII	AGATCT	1 cut(s)	1168
BmcAI	AGTACT	1 cut(s)	952
Bme1390I	CCNGG	6 cut(s)	78, 557, 742, 856, 1113, 1313
Bme18I	GGWCC	3 cut(s)	527, 1109, 1309
BmgT120I	GGNCC	3 cut(s)	527, 1109, 1309
BmiI	GGNNCC	5 cut(s)	528, 670, 860, 1110, 1264
BmrFI	CCNGG	6 cut(s)	78, 557, 742, 856, 1113, 1313
BmsI	GCATC	2 cut(s)	264, 679
BoxI	GACNNNNGTC	1 cut(s)	973
BpmI	CTGGAG	2 cut(s)	992, 1047
Bpu10I	CCTNAGC	1 cut(s)	756
Bpu14I	TTCGAA	1 cut(s)	896
BpuEI	CTTGAG	1 cut(s)	101
BpuMI	CCSGG	2 cut(s)	78, 557
BsaHI	GRCGYC	1 cut(s)	101
BsaJI	CCNNGG	4 cut(s)	68, 555, 696, 830
BsaXI	ACNNNNNCTCC	2 cut(s)	417, 447
Bsc4I	CCNNNNNNNGG	2 cut(s)	60, 422
Bse1I	ACTGG	1 cut(s)	975
Bse3DI	GCAATG	2 cut(s)	567, 915
BseBI	CCWGG	4 cut(s)	742, 856, 1113, 1313
BseDI	CCNNGG	4 cut(s)	68, 555, 696, 830
BseGI	GGATG	4 cut(s)	208, 405, 1024, 1135
BseLI	CCNNNNNNNGG	2 cut(s)	60, 422
BseMI	GCAATG	2 cut(s)	567, 915
BseMII	CTCAG	1 cut(s)	747
BseNI	ACTGG	1 cut(s)	975
BseRI	GAGGAG	2 cut(s)	744, 1044
BseXI	GCAGC	7 cut(s)	367, 397, 400, 403, 454, 457, 1146
Bsh1236I	CGCG	1 cut(s)	711
Bsh1285I	CGRYCG	1 cut(s)	709
BshFI	GGCC	4 cut(s)	64, 112, 541, 716
BshNI	GGYRCC	2 cut(s)	668, 858
BsiEI	CGRYCG	1 cut(s)	709
BsiSI	CCGG	2 cut(s)	78, 557
BslFI	GGGAC	3 cut(s)	540, 981, 1104
BslI	CCNNNNNNNGG	2 cut(s)	60, 422
BsmFI	GGGAC	3 cut(s)	540, 981, 1104
BsmI	GAATGC	1 cut(s)	742
BsnI	GGCC	4 cut(s)	64, 112, 541, 716
Bsp119I	TTCGAA	1 cut(s)	896
Bsp1286I	GDGCHC	1 cut(s)	134
Bsp143I	GATC	3 cut(s)	257, 1168, 1256
Bsp19I	CCATGG	2 cut(s)	68, 696
BspACI	CCGC	9 cut(s)	12, 65, 353, 634, 693, 709, 862, 914, 917
BspANI	GGCC	4 cut(s)	64, 112, 541, 716
BspCNI	CTCAG	1 cut(s)	748
BspFNI	CGCG	1 cut(s)	711
BspLI	GGNNCC	5 cut(s)	528, 670, 860, 1110, 1264
BspMAI	CTGCAG	1 cut(s)	1102
BspMI	ACCTGC	1 cut(s)	1091
BspPI	GGATC	2 cut(s)	265, 1264
BspT104I	TTCGAA	1 cut(s)	896
BspT107I	GGYRCC	2 cut(s)	668, 858
BsrDI	GCAATG	2 cut(s)	567, 915
BsrI	ACTGG	1 cut(s)	975
BssECI	CCNNGG	4 cut(s)	68, 555, 696, 830
BssMI	GATC	3 cut(s)	257, 1168, 1256
BssNI	GRCGYC	1 cut(s)	101
BssT1I	CCWWGG	3 cut(s)	68, 696, 830
Bst2UI	CCWGG	4 cut(s)	742, 856, 1113, 1313
Bst4CI	ACNGT	1 cut(s)	1151
Bst6I	CTCTTC	4 cut(s)	229, 936, 1273, 1282
BstACI	GRCGYC	1 cut(s)	101
BstAPI	GCANNNNNTGC	1 cut(s)	1097
BstBI	TTCGAA	1 cut(s)	896
BstC8I	GCNNGC	2 cut(s)	539, 866
BstDEI	CTNAG	1 cut(s)	756
BstDSI	CCRYGG	2 cut(s)	68, 696
BstENI	CCTNNNNNAGG	1 cut(s)	58
BstF5I	GGATG	4 cut(s)	208, 405, 1024, 1135
BstFNI	CGCG	1 cut(s)	711
BstH2I	RGCGCY	1 cut(s)	894
BstHHI	GCGC	1 cut(s)	893
BstKTI	GATC	3 cut(s)	260, 1171, 1259
BstMBI	GATC	3 cut(s)	257, 1168, 1256
BstMCI	CGRYCG	1 cut(s)	709
BstMWI	GCNNNNNNNGC	9 cut(s)	73, 98, 274, 391, 689, 698, 832, 865, 1097
BstNI	CCWGG	4 cut(s)	742, 856, 1113, 1313
BstPAI	GACNNNNGTC	1 cut(s)	973
BstSCI	CCNGG	6 cut(s)	76, 555, 740, 854, 1111, 1311
BstSFI	CTRYAG	1 cut(s)	1098
BstUI	CGCG	1 cut(s)	711
BstV1I	GCAGC	7 cut(s)	367, 397, 400, 403, 454, 457, 1146
BstX2I	RGATCY	3 cut(s)	257, 1168, 1256
BstYI	RGATCY	3 cut(s)	257, 1168, 1256
BsuRI	GGCC	4 cut(s)	64, 112, 541, 716
BtgI	CCRYGG	2 cut(s)	68, 696
BtgZI	GCGATG	2 cut(s)	184, 396
BtsCI	GGATG	4 cut(s)	208, 405, 1024, 1135
BveI	ACCTGC	1 cut(s)	1091
Cac8I	GCNNGC	2 cut(s)	539, 866
CfoI	GCGC	1 cut(s)	893
Cfr13I	GGNCC	3 cut(s)	527, 1109, 1309
CseI	GACGC	2 cut(s)	90, 485
Csp6I	GTAC	1 cut(s)	951
CviAII	CATG	4 cut(s)	69, 697, 836, 1288
CviQI	GTAC	1 cut(s)	951
DdeI	CTNAG	1 cut(s)	756
DpnI	GATC	3 cut(s)	259, 1170, 1258
DpnII	GATC	3 cut(s)	257, 1168, 1256
Eam1104I	CTCTTC	4 cut(s)	229, 936, 1273, 1282
EarI	CTCTTC	4 cut(s)	229, 936, 1273, 1282
EciI	GGCGGA	1 cut(s)	623
Eco130I	CCWWGG	3 cut(s)	68, 696, 830
Eco147I	AGGCCT	2 cut(s)	112, 716
Eco24I	GRGCYC	1 cut(s)	134
Eco47I	GGWCC	3 cut(s)	527, 1109, 1309
Eco47III	AGCGCT	1 cut(s)	892
Eco57I	CTGAAG	2 cut(s)	446, 705
EcoNI	CCTNNNNNAGG	1 cut(s)	58
EcoRI	GAATTC	1 cut(s)	1237
EcoRII	CCWGG	4 cut(s)	740, 854, 1111, 1311
EcoT14I	CCWWGG	3 cut(s)	68, 696, 830
EcoT38I	GRGCYC	1 cut(s)	134
ErhI	CCWWGG	3 cut(s)	68, 696, 830
FaeI	CATG	4 cut(s)	72, 700, 839, 1291
FalI	AAGNNNNNCTT	4 cut(s)	1068, 1100, 1283, 1315
FaqI	GGGAC	3 cut(s)	540, 981, 1104
FatI	CATG	4 cut(s)	68, 696, 835, 1287
FokI	GGATG	4 cut(s)	195, 412, 1031, 1142
FriOI	GRGCYC	1 cut(s)	134
GlaI	GCGC	1 cut(s)	892
GsuI	CTGGAG	2 cut(s)	992, 1047
HaeII	RGCGCY	1 cut(s)	894
HaeIII	GGCC	4 cut(s)	64, 112, 541, 716
HapII	CCGG	2 cut(s)	78, 557
HgaI	GACGC	2 cut(s)	90, 485
HhaI	GCGC	1 cut(s)	893
Hin1I	GRCGYC	1 cut(s)	101
Hin1II	CATG	4 cut(s)	72, 700, 839, 1291
Hin6I	GCGC	1 cut(s)	891
HinP1I	GCGC	1 cut(s)	891
HindIII	AAGCTT	1 cut(s)	156
HinfI	GANTC	7 cut(s)	374, 428, 490, 652, 962, 974, 1231
HpaII	CCGG	2 cut(s)	78, 557
HphI	GGTGA	4 cut(s)	153, 315, 445, 517
Hpy166II	GTNNAC	1 cut(s)	1040
Hpy188I	TCNGA	4 cut(s)	425, 784, 1168, 1236
Hpy188III	TCNNGA	5 cut(s)	58, 118, 127, 966, 1254
Hpy8I	GTNNAC	1 cut(s)	1040
Hpy99I	CGWCG	2 cut(s)	106, 257
HpyAV	CCTTC	7 cut(s)	64, 174, 198, 647, 727, 833, 1300
HpyCH4III	ACNGT	1 cut(s)	1151
HpyCH4V	TGCA	4 cut(s)	277, 1091, 1100, 1162
HpyF10VI	GCNNNNNNNGC	9 cut(s)	73, 98, 274, 391, 689, 698, 832, 865, 1097
HpyF3I	CTNAG	1 cut(s)	756
Hsp92I	GRCGYC	1 cut(s)	101
Hsp92II	CATG	4 cut(s)	72, 700, 839, 1291
HspAI	GCGC	1 cut(s)	891
Kzo9I	GATC	3 cut(s)	257, 1168, 1256
LmnI	GCTCC	2 cut(s)	208, 808
Lsp1109I	GCAGC	7 cut(s)	367, 397, 400, 403, 454, 457, 1146
LweI	GCATC	2 cut(s)	264, 679
MaeIII	GTNAC	2 cut(s)	473, 546
MalI	GATC	3 cut(s)	259, 1170, 1258
MboI	GATC	3 cut(s)	257, 1168, 1256
MflI	RGATCY	3 cut(s)	257, 1168, 1256
MhlI	GDGCHC	1 cut(s)	134
MlyI	GAGTC	4 cut(s)	437, 499, 646, 983
MmeI	TCCRAC	1 cut(s)	403
MroXI	GAANNNNTTC	1 cut(s)	892
MseI	TTAA	3 cut(s)	1086, 1209, 1215
MslI	CAYNNNNRTG	1 cut(s)	471
MspA1I	CMGCKG	1 cut(s)	683
MspI	CCGG	2 cut(s)	78, 557
MspR9I	CCNGG	6 cut(s)	78, 557, 742, 856, 1113, 1313
Mva1269I	GAATGC	1 cut(s)	742
MvaI	CCWGG	4 cut(s)	742, 856, 1113, 1313
MvnI	CGCG	1 cut(s)	711
MwoI	GCNNNNNNNGC	9 cut(s)	73, 98, 274, 391, 689, 698, 832, 865, 1097
NciI	CCSGG	2 cut(s)	78, 557
NcoI	CCATGG	2 cut(s)	68, 696
NdeII	GATC	3 cut(s)	257, 1168, 1256
NlaIII	CATG	4 cut(s)	72, 700, 839, 1291
NlaIV	GGNNCC	5 cut(s)	528, 670, 860, 1110, 1264
NmuCI	GTSAC	1 cut(s)	473
NspV	TTCGAA	1 cut(s)	896
PceI	AGGCCT	2 cut(s)	112, 716
PctI	GAATGC	1 cut(s)	742
PdmI	GAANNNNTTC	1 cut(s)	892
PfeI	GAWTC	3 cut(s)	374, 962, 1231
PleI	GAGTC	4 cut(s)	436, 498, 646, 982
PpsI	GAGTC	4 cut(s)	436, 498, 646, 982
PshAI	GACNNNNGTC	1 cut(s)	973
PshBI	ATTAAT	1 cut(s)	1215
PsiI	TTATAA	1 cut(s)	570
Psp6I	CCWGG	4 cut(s)	740, 854, 1111, 1311
PspGI	CCWGG	4 cut(s)	740, 854, 1111, 1311
PspN4I	GGNNCC	5 cut(s)	528, 670, 860, 1110, 1264
PspPI	GGNCC	3 cut(s)	527, 1109, 1309
PstI	CTGCAG	1 cut(s)	1102
PsuI	RGATCY	3 cut(s)	257, 1168, 1256
PvuII	CAGCTG	1 cut(s)	683
RsaI	GTAC	1 cut(s)	952
RsaNI	GTAC	1 cut(s)	951
RseI	CAYNNNNRTG	1 cut(s)	471
SaqAI	TTAA	3 cut(s)	1086, 1209, 1215
Sau3AI	GATC	3 cut(s)	257, 1168, 1256
Sau96I	GGNCC	3 cut(s)	527, 1109, 1309
SbfI	CCTGCAGG	1 cut(s)	1102
ScaI	AGTACT	1 cut(s)	952
SchI	GAGTC	4 cut(s)	437, 499, 646, 983
ScrFI	CCNGG	6 cut(s)	78, 557, 742, 856, 1113, 1313
SdaI	CCTGCAGG	1 cut(s)	1102
SduI	GDGCHC	1 cut(s)	134
SfaNI	GCATC	2 cut(s)	264, 679
SfcI	CTRYAG	1 cut(s)	1098
SfuI	TTCGAA	1 cut(s)	896
SinI	GGWCC	3 cut(s)	527, 1109, 1309
SmiMI	CAYNNNNRTG	1 cut(s)	471
SmlI	CTYRAG	1 cut(s)	116
SmoI	CTYRAG	1 cut(s)	116
Sse8387I	CCTGCAGG	1 cut(s)	1102
SseBI	AGGCCT	2 cut(s)	112, 716
SsiI	CCGC	9 cut(s)	12, 65, 353, 634, 693, 709, 862, 914, 917
StuI	AGGCCT	2 cut(s)	112, 716
StyD4I	CCNGG	6 cut(s)	76, 555, 740, 854, 1111, 1311
StyI	CCWWGG	3 cut(s)	68, 696, 830
TaaI	ACNGT	1 cut(s)	1151
TaqI	TCGA	5 cut(s)	57, 104, 252, 643, 896
TatI	WGTACW	1 cut(s)	950
TauI	GCSGC	4 cut(s)	67, 695, 916, 919
TfiI	GAWTC	3 cut(s)	374, 962, 1231
Tru1I	TTAA	3 cut(s)	1086, 1209, 1215
Tru9I	TTAA	3 cut(s)	1086, 1209, 1215
TseFI	GTSAC	1 cut(s)	473
TseI	GCWGC	7 cut(s)	355, 385, 388, 391, 442, 445, 1159
Tsp45I	GTSAC	1 cut(s)	473
TspDTI	ATGAA	5 cut(s)	530, 786, 852, 1269, 1304
TspGWI	ACGGA	2 cut(s)	270, 1031
VpaK11BI	GGWCC	3 cut(s)	527, 1109, 1309
VspI	ATTAAT	1 cut(s)	1215
XagI	CCTNNNNNAGG	1 cut(s)	58
XapI	RAATTY	3 cut(s)	304, 484, 1237
XmnI	GAANNNNTTC	1 cut(s)	892
ZrmI	AGTACT	1 cut(s)	952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.