Rroxscaffold_2G00117280

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013015
Physical Location & Seq
Forward (+)
44797283 .. 44798698
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00117280.1

Sequence Viewer

Length: 1137 bp
ATGGCCACAGCAAAAAGGAGTGGTGAATCAAACATTGAAACTGGGATGGAAACAAGCCGAGATTGTGACCTTGACAAGGGCAAGGAACATGATTTTTTCTTGGAGAGTTGTCAATGGAAGCCCGTTTTTGATGAAGCTTCAATGTCACATAGACCTCTTAAGAAGATCAAGAGCCCTGAACGTAGTCATCAGCACCAATCCTCTGCTTTTCCCCCTGAATTTCATATCCCATCTTCTCCTCCTCGTAGTTCATCAGATACATCATCAATCACTTCTTGTTGTCCTTCTTCTTCCAGAATAGTCTTTCCTTTTGCTTTTGATGGGTTTGGAGCAGCTAATAGTAGTGGTGGTATTAGGCCAGCGGCGATGCCTGTAAGTACTAGTAAGCTTTACAGGGGAGTAAGGCAAAGGCATTGGGGCAAATGGGTAGCTGAAATTCGTCTTCCTCGAAACAGGACTCGCCTCTGGCTGGGCACCTTCGACACCGCTGAAGATGCTGCCATGGCTTATGACCGTGAGGCTTTCAAGTTGAGAGGAGAGAATGCTAGGCTTAATTTCCCTGAGCTTTTCCTCAACAAAGACAAAGATAATAATAATGCTGCAGCTGTTTCCACCGCTCCAAGTTCAACAGAAACTGCTTCTGTGCCGACTCAACAAGTTTCAAAGCCATCCAGGCACCAGGGTAAGCCGCAACTGAAAAACCTTGATTTACAATTACAGGTTCCCATTTCAGATAGGGAAACAATTGCACCTCCACCAGCGCCATATTCACATCCCCAGGAAGACGATGACACTGGTGAAAGTGATTTGGCCAATGCCAGCCAGCAATCTGAGGTTGAAAATCATGGCGAAGGAGGAGGGATCATTTCAGATGCCCCAACAGAGGAAATGGCTTGGGGAGAAATGGCAGAGGCTTGGATGAATGCAATTCCACAAGGTTGGGGTCCTGGTAGTCCTGTCTGGGATGATTTAGAGTTGGACATGACTTCCAACAATCTTGTGCTGCAATCTCATGATTATCACCATTTTACGGCCAATAATCCGAACCGGCATGAACTTCCGCAGCAGGAAGACAACTCTTGTTCTGCTTCATCTTCATATCCCATGCAGCCCTTCTTTTGGAAAGATCAAGATTGA

Protein Analysis

378

Amino Acids

41.97

Weight (kDa)

5.21

Isoelectric Point (pI)

65.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 130 - 179 1.4e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20880 AT4G28140
fragaria_vesca FvH4_6g26090
malus_domestica MD09G1252000.v1.1 MD17G1244300.v1.1
prunus_persica Prupe.3G157100_v2.0.a1
pyrus_communis pycom09g16830 pycom17g24710
rosa_chinensis RchiOBHm_Chr2g0126301
rosa_laevigata RLG00000018898
rosa_multiflora Rmu_ssc0000264.1_g000034
rosa_roxburghii Rroxscaffold_2G00117280
rosa_rugosa Rorug02G0262400
rosa_samantha Rh2AG319300 Rh2BG327700 Rh2CG307700 Rh2DG347300
rosa_wichuraiana Rw2G025870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 675
AccBSI CCGCTC 1 cut(s) 617
AciI CCGC 5 cut(s) 362, 486, 615, 689, 1061
AclWI GGATC 1 cut(s) 869
AcoI YGGCCR 3 cut(s) 3, 810, 1032
AcsI RAATTY 2 cut(s) 218, 435
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 5 cut(s) 76, 469, 883, 1030, 1119
AflII CTTAAG 1 cut(s) 158
AgsI TTSAA 6 cut(s) 38, 141, 526, 627, 663, 839
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 4 cut(s) 671, 678, 777, 946
AluBI AGCT 6 cut(s) 137, 335, 388, 431, 565, 605
AluI AGCT 6 cut(s) 137, 335, 388, 431, 565, 605
AlwI GGATC 1 cut(s) 869
AlwNI CAGNNNCTG 1 cut(s) 635
AoxI GGCC 4 cut(s) 3, 356, 810, 1032
ApeKI GCWGC 7 cut(s) 332, 497, 599, 602, 1003, 1063, 1108
ApoI RAATTY 2 cut(s) 218, 435
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 944
AsuHPI GGTGA 3 cut(s) 35, 809, 1013
AvaII GGWCC 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 476
BalI TGGCCA 2 cut(s) 5, 812
BanI GGYRCC 2 cut(s) 473, 675
BanII GRGCYC 1 cut(s) 176
BbsI GAAGAC 3 cut(s) 434, 789, 1077
BbvI GCAGC 7 cut(s) 344, 484, 586, 614, 990, 1075, 1120
BccI CCATC 4 cut(s) 40, 238, 314, 676
BceAI ACGGC 1 cut(s) 1047
BciT130I CCWGG 4 cut(s) 673, 680, 779, 948
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 2 cut(s) 381, 546
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 764
BfrI CTTAAG 1 cut(s) 158
BglI GCCNNNNNGGC 1 cut(s) 673
BisI GCNGC 9 cut(s) 333, 363, 498, 600, 603, 689, 1004, 1064, 1109
BlsI GCNGC 9 cut(s) 334, 364, 499, 601, 604, 690, 1005, 1065, 1110
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 4 cut(s) 673, 680, 779, 948
Bme18I GGWCC 1 cut(s) 944
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 4 cut(s) 475, 677, 723, 945
BmrFI CCNGG 4 cut(s) 673, 680, 779, 948
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 3 cut(s) 357, 484, 862
BmuI ACTGGG 1 cut(s) 51
BpiI GAAGAC 3 cut(s) 434, 789, 1077
Bpu10I CCTNAGC 1 cut(s) 561
BsaJI CCNNGG 3 cut(s) 501, 679, 777
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 469, 883, 1030, 1119
Bse118I RCCGGY 1 cut(s) 1047
Bse1I ACTGG 2 cut(s) 46, 799
BseBI CCWGG 4 cut(s) 673, 680, 779, 948
BseDI CCNNGG 3 cut(s) 501, 679, 777
BseGI GGATG 5 cut(s) 51, 668, 772, 924, 970
BseLI CCNNNNNNNGG 5 cut(s) 76, 469, 883, 1030, 1119
BseMII CTCAG 2 cut(s) 552, 822
BseNI ACTGG 2 cut(s) 46, 799
BseRI GAGGAG 4 cut(s) 228, 231, 549, 870
BseSI GKGCMC 1 cut(s) 476
BseXI GCAGC 7 cut(s) 344, 484, 586, 614, 990, 1075, 1120
BseYI CCCAGC 1 cut(s) 469
BshFI GGCC 4 cut(s) 5, 358, 812, 1034
BshNI GGYRCC 2 cut(s) 473, 675
BsiSI CCGG 1 cut(s) 1048
BslI CCNNNNNNNGG 5 cut(s) 76, 469, 883, 1030, 1119
BsmI GAATGC 2 cut(s) 547, 928
BsnI GGCC 4 cut(s) 5, 358, 812, 1034
Bsp1286I GDGCHC 2 cut(s) 176, 476
Bsp143I GATC 3 cut(s) 165, 861, 1126
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 5 cut(s) 362, 486, 615, 689, 1061
BspANI GGCC 4 cut(s) 5, 358, 812, 1034
BspCNI CTCAG 2 cut(s) 553, 823
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 4 cut(s) 475, 677, 723, 945
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 869
BspT107I GGYRCC 2 cut(s) 473, 675
BspTI CTTAAG 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 1047
BsrI ACTGG 2 cut(s) 46, 799
BssAI RCCGGY 1 cut(s) 1047
BssECI CCNNGG 3 cut(s) 501, 679, 777
BssMI GATC 3 cut(s) 165, 861, 1126
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 4 cut(s) 673, 680, 779, 948
Bst4CI ACNGT 1 cut(s) 515
BstAFI CTTAAG 1 cut(s) 158
BstC8I GCNNGC 3 cut(s) 360, 820, 824
BstDEI CTNAG 2 cut(s) 561, 831
BstDSI CCRYGG 1 cut(s) 501
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 5 cut(s) 51, 668, 772, 924, 970
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 3 cut(s) 168, 864, 1129
BstMBI GATC 3 cut(s) 165, 861, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 494, 503, 673
BstNI CCWGG 4 cut(s) 673, 680, 779, 948
BstSCI CCNGG 4 cut(s) 671, 678, 777, 946
BstSFI CTRYAG 1 cut(s) 600
BstSLI GKGCMC 1 cut(s) 476
BstV1I GCAGC 7 cut(s) 344, 484, 586, 614, 990, 1075, 1120
BstV2I GAAGAC 3 cut(s) 434, 789, 1077
BstXI CCANNNNNNTGG 1 cut(s) 939
BsuRI GGCC 4 cut(s) 5, 358, 812, 1034
BtgI CCRYGG 1 cut(s) 501
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 5 cut(s) 51, 668, 772, 924, 970
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 3 cut(s) 360, 820, 824
CaiI CAGNNNCTG 1 cut(s) 635
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 1047
Cfr13I GGNCC 1 cut(s) 944
Csp6I GTAC 1 cut(s) 378
CviAII CATG 7 cut(s) 89, 502, 845, 982, 1013, 1052, 1105
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 2 cut(s) 561, 831
DpnI GATC 3 cut(s) 167, 863, 1128
DpnII GATC 3 cut(s) 165, 861, 1126
EaeI YGGCCR 3 cut(s) 3, 810, 1032
Eco130I CCWWGG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 944
Eco57I CTGAAG 1 cut(s) 510
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 944
EcoRII CCWGG 4 cut(s) 671, 678, 777, 946
EcoT14I CCWWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 7 cut(s) 92, 505, 848, 985, 1016, 1055, 1108
FatI CATG 7 cut(s) 88, 501, 844, 981, 1012, 1051, 1104
Fnu4HI GCNGC 9 cut(s) 333, 363, 498, 600, 603, 689, 1004, 1064, 1109
FokI GGATG 5 cut(s) 58, 655, 759, 931, 977
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 9 cut(s) 333, 363, 498, 600, 603, 689, 1004, 1064, 1109
FspBI CTAG 2 cut(s) 381, 546
GlaI GCGC 1 cut(s) 762
GluI GCNGC 9 cut(s) 333, 363, 498, 600, 603, 689, 1004, 1064, 1109
GsaI CCCAGC 1 cut(s) 473
HaeII RGCGCY 1 cut(s) 764
HaeIII GGCC 4 cut(s) 5, 358, 812, 1034
HapII CCGG 1 cut(s) 1048
HhaI GCGC 1 cut(s) 763
Hin1II CATG 7 cut(s) 92, 505, 848, 985, 1016, 1055, 1108
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HindIII AAGCTT 2 cut(s) 135, 386
HinfI GANTC 3 cut(s) 26, 457, 649
HpaII CCGG 1 cut(s) 1048
HphI GGTGA 3 cut(s) 35, 809, 1013
Hpy188I TCNGA 5 cut(s) 256, 733, 832, 871, 1044
Hpy188III TCNNGA 4 cut(s) 169, 294, 1013, 1130
HpyAV CCTTC 4 cut(s) 294, 487, 845, 1123
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 5 cut(s) 602, 749, 926, 1006, 1108
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 503, 673
HpyF3I CTNAG 2 cut(s) 561, 831
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 7 cut(s) 92, 505, 848, 985, 1016, 1055, 1108
HspAI GCGC 1 cut(s) 761
Kzo9I GATC 3 cut(s) 165, 861, 1126
LmnI GCTCC 2 cut(s) 329, 622
Lsp1109I GCAGC 7 cut(s) 344, 484, 586, 614, 990, 1075, 1120
LweI GCATC 3 cut(s) 357, 484, 862
MaeI CTAG 2 cut(s) 381, 546
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 2 cut(s) 65, 144
MalI GATC 3 cut(s) 167, 863, 1128
MbiI CCGCTC 1 cut(s) 617
MboI GATC 3 cut(s) 165, 861, 1126
MboII GAAGA 9 cut(s) 175, 225, 279, 282, 434, 503, 794, 1082, 1086
MfeI CAATTG 1 cut(s) 744
MhlI GDGCHC 2 cut(s) 176, 476
MlsI TGGCCA 2 cut(s) 5, 812
MluCI AATT 6 cut(s) 218, 435, 553, 713, 744, 927
MluNI TGGCCA 2 cut(s) 5, 812
MlyI GAGTC 2 cut(s) 451, 643
MmeI TCCRAC 2 cut(s) 957, 1014
Mox20I TGGCCA 2 cut(s) 5, 812
MscI TGGCCA 2 cut(s) 5, 812
MseI TTAA 2 cut(s) 159, 552
Msp20I TGGCCA 2 cut(s) 5, 812
MspA1I CMGCKG 3 cut(s) 362, 488, 605
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 1 cut(s) 1048
MspR9I CCNGG 4 cut(s) 673, 680, 779, 948
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 547, 928
MvaI CCWGG 4 cut(s) 673, 680, 779, 948
MwoI GCNNNNNNNGC 3 cut(s) 494, 503, 673
NcoI CCATGG 1 cut(s) 501
NdeII GATC 3 cut(s) 165, 861, 1126
NlaIII CATG 7 cut(s) 92, 505, 848, 985, 1016, 1055, 1108
NlaIV GGNNCC 4 cut(s) 475, 677, 723, 945
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 2 cut(s) 65, 144
PagI TCATGA 1 cut(s) 1012
PcsI WCGNNNNNNNCGW 1 cut(s) 445
PctI GAATGC 2 cut(s) 547, 928
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 9 cut(s) 334, 364, 499, 601, 604, 690, 1005, 1065, 1110
PleI GAGTC 2 cut(s) 451, 643
PpsI GAGTC 2 cut(s) 451, 643
PpuMI RGGWCCY 1 cut(s) 944
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 4 cut(s) 671, 678, 777, 946
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 4 cut(s) 671, 678, 777, 946
PspN4I GGNNCC 4 cut(s) 475, 677, 723, 945
PspPI GGNCC 1 cut(s) 944
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 1 cut(s) 635
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 159, 552
SatI GCNGC 9 cut(s) 333, 363, 498, 600, 603, 689, 1004, 1064, 1109
Sau3AI GATC 3 cut(s) 165, 861, 1126
Sau96I GGNCC 1 cut(s) 944
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 451, 643
ScrFI CCNGG 4 cut(s) 673, 680, 779, 948
SduI GDGCHC 2 cut(s) 176, 476
SfaNI GCATC 3 cut(s) 357, 484, 862
SfcI CTRYAG 1 cut(s) 600
SinI GGWCC 1 cut(s) 944
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 6 cut(s) 218, 435, 553, 713, 744, 927
SsiI CCGC 5 cut(s) 362, 486, 615, 689, 1061
SspMI CTAG 2 cut(s) 381, 546
StyD4I CCNGG 4 cut(s) 671, 678, 777, 946
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 448, 480
TasI AATT 6 cut(s) 218, 435, 553, 713, 744, 927
TatI WGTACW 1 cut(s) 377
TauI GCSGC 2 cut(s) 365, 691
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 159, 552
Tru9I TTAA 2 cut(s) 159, 552
TscAI CASTG 1 cut(s) 799
TseFI GTSAC 2 cut(s) 65, 144
TseI GCWGC 7 cut(s) 332, 497, 599, 602, 1003, 1063, 1108
Tsp45I GTSAC 2 cut(s) 65, 144
TspDTI ATGAA 7 cut(s) 147, 212, 240, 935, 1068, 1080, 1086
TspRI CASTG 1 cut(s) 799
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 944
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 2 cut(s) 218, 435
XspI CTAG 2 cut(s) 381, 546
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.