pycom10g06360

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009119
Physical Location & Seq
Forward (+)
7341671 .. 7345287
3617 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1353 bp
ATGTCAGCCAAAAGAATTGTTATCAGTGGAAGTCCCACTACGGAGTCGCCTGGAGATAACACCTACACAGCAACAATGGAGGAAAGCAGTGCAGTGAGGCGACTGGATTCAATTTCTCGTCACCTTCTATTGCAACCTCCTCAACCTAACCATGGCCTTCAACAGGCGGTGTTATTAAGTAATGGGAAATGGGAAAGTGAAGGGTCAGAGGCTGTGGTGATAGGAGGCATGGTGTTGGACATTCATGCCACACCTTCCATCCCTTCAAATCCCAGAACCACCACTCCTGGCAAGGTCAGCTATGTAGGAGGAGGGGTAGCAAGGAATGTTGCCGAGTGCATGTCAAAGCTAGGAGCTAAGCCCTTCATGATAAGCGCCTTGGGCCTTGACATGGCAGGAAACTTATTGTTGGAGCACTGGAAGTCTTCTGGGCTATCTATGGAAGGCATTTTGAGGCACCAAAATGTTGAGACTCCTGTTGTGTGCAACGTACTTGATGTTAATGGGGAGGTGGCCGCTGGTGTTGCGAGTGTAGAAGCTCTCGAAAAGTTTCTGACGCCCGAGTGGATCCAACAGTTTAAATACAGTATACGGTCTGCTCCAGTGTTGATGATTGATGCAAACCTGAATCTTTCTGCTCTAAAAGCTTCTTGCCAATTGGCAGCAGAATCTAAAATTCCAGTGTGGTTTGAGCCTGTATCGGTAACAAAATCTAGAAGAATCAATTCAATTTCCAAGTATGTAAGTTTTGCTTCACCCAACGAGGATGAACTTGTTGCCATGGCAAATGCTTTATCCGGTGGAAATGTGTTTTCTCCAATTGAAAGGGATCAATGCTCAACCGAAACTTTGTTCCATATGCTAAAACCTGCAGTCTGGGTTTTGCTAGAGAAAGGTATCAAAATAGTTGTTGTGACCGTTGGTTCAGATGGAGTGTTCTTATGCTCTAAAGGAGGGCCAAGCTTCATGAGAACTGTTTGCAAGGGAAACAAACCATATGTTTCCAATGCGGAATTGTTTAACACTGTGACTGCAAGTTGTCCATCAAATGCGTTCTCAAATCCTTTAGATTCTGACAAAAGCTCGTTTCTCTTTGCTGTGCAATTTCCTGCACTTCCTGCATCGGTGGTAAGGCTTACAGGAGCTGGCGATTGCTTGGTTGGGGGGACTGTTGCTTCCATTTGTGCAGGTTTAGATGTCATGCAAAGTCTAGCAGTTGGTATTGCAGCTTCCAAAGCTGCAGTTGAGGCAGAGACCAATGTGCCTCGTGTACTTAATCTGGCCGCAATAGCAGATGATGCAAAGTTGGTACACTCGGCTGCAAAAGTTGTGTTCCATCAATCAGTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MaoC_dehydratas PfkB

Protein Analysis

451

Amino Acids

47.51

Weight (kDa)

6.27

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 71 - 326 3.2e-26 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 877, 1178
AccB1I GGYRCC 1 cut(s) 456
AccI GTMKAC 1 cut(s) 589
AciI CCGC 4 cut(s) 167, 516, 1010, 1284
AclWI GGATC 3 cut(s) 562, 575, 837
AcoI YGGCCR 2 cut(s) 513, 1281
AcsI RAATTY 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 557
AfaI GTAC 3 cut(s) 492, 1272, 1311
AfiI CCNNNNNNNGG 3 cut(s) 152, 163, 391
AgsI TTSAA 5 cut(s) 111, 161, 267, 729, 824
AjnI CCWGG 2 cut(s) 49, 286
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 464, 1247
AlwI GGATC 3 cut(s) 562, 575, 837
AlwNI CAGNNNCTG 2 cut(s) 212, 1145
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 5 cut(s) 154, 382, 513, 956, 1281
ApeKI GCWGC 4 cut(s) 662, 1226, 1238, 1319
ApoI RAATTY 1 cut(s) 675
Asp700I GAANNNNTTC 2 cut(s) 549, 724
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 2 cut(s) 382, 956
AsuHPI GGTGA 3 cut(s) 113, 229, 747
AvaI CYCGRG 1 cut(s) 560
BamHI GGATCC 1 cut(s) 567
BanI GGYRCC 1 cut(s) 456
BarI GAAGNNNNNNTAC 4 cut(s) 22, 54, 1213, 1245
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 4 cut(s) 674, 1225, 1238, 1306
BccI CCATC 4 cut(s) 266, 923, 1051, 1344
BciT130I CCWGG 2 cut(s) 51, 288
BcoDI GTCTC 2 cut(s) 464, 1247
BfaI CTAG 4 cut(s) 350, 714, 887, 1211
BfmI CTRYAG 2 cut(s) 870, 1239
BfoI RGCGCY 1 cut(s) 378
BfuAI ACCTGC 2 cut(s) 877, 1178
BisI GCNGC 6 cut(s) 516, 663, 1227, 1239, 1284, 1320
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 6 cut(s) 517, 664, 1228, 1240, 1285, 1321
Bme1390I CCNGG 2 cut(s) 51, 288
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 2 cut(s) 382, 956
BmiI GGNNCC 2 cut(s) 458, 569
BmrFI CCNGG 2 cut(s) 51, 288
BmsI GCATC 3 cut(s) 607, 1130, 1288
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 2 cut(s) 72, 585
Bpu1102I GCTNAGC 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 1247
BsaJI CCNNGG 3 cut(s) 151, 378, 780
BsaWI WCCGGW 1 cut(s) 797
BsaXI ACNNNNNCTCC 6 cut(s) 216, 246, 268, 298, 300, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 163, 391
Bse1I ACTGG 4 cut(s) 108, 422, 602, 680
BseBI CCWGG 2 cut(s) 51, 288
BseDI CCNNGG 3 cut(s) 151, 378, 780
BseGI GGATG 2 cut(s) 258, 772
BseLI CCNNNNNNNGG 3 cut(s) 152, 163, 391
BseNI ACTGG 4 cut(s) 108, 422, 602, 680
BseRI GAGGAG 2 cut(s) 129, 324
BseXI GCAGC 4 cut(s) 674, 1225, 1238, 1306
BsgI GTGCAG 3 cut(s) 111, 1095, 1206
BshFI GGCC 5 cut(s) 156, 384, 515, 958, 1283
BshNI GGYRCC 1 cut(s) 456
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 1 cut(s) 798
BslFI GGGAC 2 cut(s) 18, 1180
BslI CCNNNNNNNGG 3 cut(s) 152, 163, 391
BsmAI GTCTC 2 cut(s) 464, 1247
BsmFI GGGAC 2 cut(s) 18, 1180
BsnI GGCC 5 cut(s) 156, 384, 515, 958, 1283
Bso31I GGTCTC 1 cut(s) 1247
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 2 cut(s) 567, 829
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 2 cut(s) 151, 780
BspACI CCGC 4 cut(s) 167, 516, 1010, 1284
BspANI GGCC 5 cut(s) 156, 384, 515, 958, 1283
BspHI TCATGA 2 cut(s) 366, 966
BspLI GGNNCC 2 cut(s) 458, 569
BspMAI CTGCAG 2 cut(s) 874, 1243
BspMI ACCTGC 2 cut(s) 877, 1178
BspPI GGATC 3 cut(s) 562, 575, 837
BspT107I GGYRCC 1 cut(s) 456
BspTNI GGTCTC 1 cut(s) 1247
BsrI ACTGG 4 cut(s) 108, 422, 602, 680
BssECI CCNNGG 3 cut(s) 151, 378, 780
BssMI GATC 2 cut(s) 567, 829
BssNAI GTATAC 1 cut(s) 590
BssNI GRCGYC 1 cut(s) 557
BssSI CACGAG 1 cut(s) 1266
BssT1I CCWWGG 3 cut(s) 151, 378, 780
Bst1107I GTATAC 1 cut(s) 590
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 2 cut(s) 51, 288
Bst4CI ACNGT 7 cut(s) 576, 587, 594, 919, 976, 1027, 1171
BstACI GRCGYC 1 cut(s) 557
BstAPI GCANNNNNTGC 2 cut(s) 1118, 1298
BstC8I GCNNGC 1 cut(s) 1147
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 2 cut(s) 151, 780
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 2 cut(s) 258, 772
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 2 cut(s) 570, 832
BstMAI GTCTC 2 cut(s) 464, 1247
BstMBI GATC 2 cut(s) 567, 829
BstMWI GCNNNNNNNGC 9 cut(s) 297, 381, 524, 644, 1118, 1235, 1247, 1289, 1298
BstNI CCWGG 2 cut(s) 51, 288
BstNSI RCATGY 1 cut(s) 343
BstSCI CCNGG 2 cut(s) 49, 286
BstSFI CTRYAG 2 cut(s) 870, 1239
BstV1I GCAGC 4 cut(s) 674, 1225, 1238, 1306
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 567
BstYI RGATCY 1 cut(s) 567
BstZ17I GTATAC 1 cut(s) 590
BsuRI GGCC 5 cut(s) 156, 384, 515, 958, 1283
BtgI CCRYGG 2 cut(s) 151, 780
BtsCI GGATG 2 cut(s) 258, 772
BtsI GCAGTG 2 cut(s) 94, 99
BtsIMutI CAGTG 8 cut(s) 31, 94, 99, 415, 609, 687, 1023, 1350
BveI ACCTGC 2 cut(s) 877, 1178
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 2 cut(s) 212, 1145
CciI TCATGA 2 cut(s) 366, 966
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 382, 956
CseI GACGC 1 cut(s) 565
Csp6I GTAC 3 cut(s) 491, 1271, 1310
CviAII CATG 9 cut(s) 152, 229, 245, 340, 367, 391, 781, 967, 1201
CviQI GTAC 3 cut(s) 491, 1271, 1310
DdeI CTNAG 1 cut(s) 357
DpnI GATC 2 cut(s) 569, 831
DpnII GATC 2 cut(s) 567, 829
DraI TTTAAA 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 513, 1281
Eco130I CCWWGG 3 cut(s) 151, 378, 780
Eco31I GGTCTC 1 cut(s) 1247
Eco88I CYCGRG 1 cut(s) 560
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 49, 286
EcoT14I CCWWGG 3 cut(s) 151, 378, 780
ErhI CCWWGG 3 cut(s) 151, 378, 780
FaeI CATG 9 cut(s) 155, 232, 248, 343, 370, 394, 784, 970, 1204
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FaqI GGGAC 2 cut(s) 18, 1180
FatI CATG 9 cut(s) 151, 228, 244, 339, 366, 390, 780, 966, 1200
FauNDI CATATG 2 cut(s) 858, 997
FblI GTMKAC 1 cut(s) 589
Fnu4HI GCNGC 6 cut(s) 516, 663, 1227, 1239, 1284, 1320
FokI GGATG 2 cut(s) 245, 779
Fsp4HI GCNGC 6 cut(s) 516, 663, 1227, 1239, 1284, 1320
FspBI CTAG 4 cut(s) 350, 714, 887, 1211
GlaI GCGC 1 cut(s) 376
GluI GCNGC 6 cut(s) 516, 663, 1227, 1239, 1284, 1320
GsuI CTGGAG 2 cut(s) 72, 585
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 5 cut(s) 156, 384, 515, 958, 1283
HapII CCGG 1 cut(s) 798
HgaI GACGC 1 cut(s) 565
HhaI GCGC 1 cut(s) 377
Hin1I GRCGYC 1 cut(s) 557
Hin1II CATG 9 cut(s) 155, 232, 248, 343, 370, 394, 784, 970, 1204
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HindIII AAGCTT 2 cut(s) 645, 961
HinfI GANTC 7 cut(s) 44, 107, 472, 628, 668, 720, 1070
HpaII CCGG 1 cut(s) 798
HphI GGTGA 3 cut(s) 113, 229, 747
Hpy166II GTNNAC 3 cut(s) 590, 1271, 1312
Hpy188I TCNGA 4 cut(s) 208, 555, 928, 1075
Hpy188III TCNNGA 4 cut(s) 367, 542, 714, 967
Hpy8I GTNNAC 3 cut(s) 590, 1271, 1312
HpyAV CCTTC 7 cut(s) 134, 167, 194, 264, 273, 373, 437
HpyCH4III ACNGT 7 cut(s) 576, 587, 594, 919, 976, 1027, 1171
HpyCH4IV ACGT 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 9 cut(s) 297, 381, 524, 644, 1118, 1235, 1247, 1289, 1298
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 489
Hsp92I GRCGYC 1 cut(s) 557
Hsp92II CATG 9 cut(s) 155, 232, 248, 343, 370, 394, 784, 970, 1204
HspAI GCGC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 567, 829
LmnI GCTCC 4 cut(s) 353, 412, 604, 1142
Lsp1109I GCAGC 4 cut(s) 674, 1225, 1238, 1306
LweI GCATC 3 cut(s) 607, 1130, 1288
MaeI CTAG 4 cut(s) 350, 714, 887, 1211
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 4 cut(s) 119, 703, 913, 1027
MalI GATC 2 cut(s) 569, 831
MboI GATC 2 cut(s) 567, 829
MboII GAAGA 2 cut(s) 417, 729
MfeI CAATTG 2 cut(s) 656, 819
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 9 cut(s) 15, 111, 656, 675, 724, 729, 819, 1013, 1103
MlyI GAGTC 2 cut(s) 53, 466
MmeI TCCRAC 3 cut(s) 216, 390, 595
MroXI GAANNNNTTC 2 cut(s) 549, 724
MseI TTAA 6 cut(s) 176, 501, 579, 1020, 1275, 1351
MslI CAYNNNNRTG 1 cut(s) 462
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 1 cut(s) 798
MspR9I CCNGG 2 cut(s) 51, 288
MunI CAATTG 2 cut(s) 656, 819
MvaI CCWGG 2 cut(s) 51, 288
MwoI GCNNNNNNNGC 9 cut(s) 297, 381, 524, 644, 1118, 1235, 1247, 1289, 1298
NcoI CCATGG 2 cut(s) 151, 780
NdeI CATATG 2 cut(s) 858, 997
NdeII GATC 2 cut(s) 567, 829
NlaIII CATG 9 cut(s) 155, 232, 248, 343, 370, 394, 784, 970, 1204
NlaIV GGNNCC 2 cut(s) 458, 569
NmeAIII GCCGAG 2 cut(s) 358, 1295
NmuCI GTSAC 3 cut(s) 119, 913, 1027
NspI RCATGY 1 cut(s) 343
PagI TCATGA 2 cut(s) 366, 966
PdmI GAANNNNTTC 2 cut(s) 549, 724
PfeI GAWTC 5 cut(s) 107, 628, 668, 720, 1070
PkrI GCNGC 6 cut(s) 517, 664, 1228, 1240, 1285, 1321
PleI GAGTC 2 cut(s) 52, 466
PpsI GAGTC 2 cut(s) 52, 466
Psp6I CCWGG 2 cut(s) 49, 286
PspGI CCWGG 2 cut(s) 49, 286
PspN4I GGNNCC 2 cut(s) 458, 569
PspPI GGNCC 2 cut(s) 382, 956
PstI CTGCAG 2 cut(s) 874, 1243
PstNI CAGNNNCTG 2 cut(s) 212, 1145
PsuI RGATCY 1 cut(s) 567
RsaI GTAC 3 cut(s) 492, 1272, 1311
RsaNI GTAC 3 cut(s) 491, 1271, 1310
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 6 cut(s) 176, 501, 579, 1020, 1275, 1351
SatI GCNGC 6 cut(s) 516, 663, 1227, 1239, 1284, 1320
Sau3AI GATC 2 cut(s) 567, 829
Sau96I GGNCC 2 cut(s) 382, 956
SchI GAGTC 2 cut(s) 53, 466
ScrFI CCNGG 2 cut(s) 51, 288
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 3 cut(s) 607, 1130, 1288
SfcI CTRYAG 2 cut(s) 870, 1239
SmiMI CAYNNNNRTG 1 cut(s) 462
Sse9I AATT 9 cut(s) 15, 111, 656, 675, 724, 729, 819, 1013, 1103
SsiI CCGC 4 cut(s) 167, 516, 1010, 1284
SspMI CTAG 4 cut(s) 350, 714, 887, 1211
StyD4I CCNGG 2 cut(s) 49, 286
StyI CCWWGG 3 cut(s) 151, 378, 780
TaaI ACNGT 7 cut(s) 576, 587, 594, 919, 976, 1027, 1171
TaiI ACGT 1 cut(s) 492
TaqI TCGA 1 cut(s) 543
TasI AATT 9 cut(s) 15, 111, 656, 675, 724, 729, 819, 1013, 1103
TatI WGTACW 1 cut(s) 1270
TauI GCSGC 2 cut(s) 518, 1286
TfiI GAWTC 5 cut(s) 107, 628, 668, 720, 1070
Tru1I TTAA 6 cut(s) 176, 501, 579, 1020, 1275, 1351
Tru9I TTAA 6 cut(s) 176, 501, 579, 1020, 1275, 1351
TscAI CASTG 8 cut(s) 31, 94, 99, 422, 609, 687, 1030, 1350
TseFI GTSAC 3 cut(s) 119, 913, 1027
TseI GCWGC 4 cut(s) 662, 1226, 1238, 1319
Tsp45I GTSAC 3 cut(s) 119, 913, 1027
TspDTI ATGAA 4 cut(s) 233, 355, 783, 955
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 8 cut(s) 31, 94, 99, 422, 609, 687, 1030, 1350
XagI CCTNNNNNAGG 1 cut(s) 161
XapI RAATTY 1 cut(s) 675
XbaI TCTAGA 1 cut(s) 713
XceI RCATGY 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 589
XmnI GAANNNNTTC 2 cut(s) 549, 724
XspI CTAG 4 cut(s) 350, 714, 887, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.