pycom10g07030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008704
Physical Location & Seq
Forward (+)
8248250 .. 8251460
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1176 bp
ATGGAGGAAGAAGACAAACAGCCGCTCAAGGAGGACAAAGGTCCTCAACAGGAAGAGAAAGAAGAACCAAAAGGAGGAGCAGCTGAAGCACAAGTGTCGCGGGCGAAAAGTGGCGGAGGATGGGGAGGTTGGGGGTTTTCGTCCGTCTTCTCAGATCTCCAGAAAGCCGCCGAAGAGATCTCCCGCAATGCGGCTGCGACTGCTCAGACGGCAGCGAAGAGCCTTGCCGACATGCAAGCTGCTGAGGACTCAGAAGAGTGTTCCAAGGAGGAAGAAGGGGATGCTGAAAAGGAAACCCAAGATGACACTGAGAGCCAAGATGAGCATGAAAAGCAAAGAAAGGCTGCTCTTGATAAATTGGAGAAAGCTAGTGAGGATTCACTTTTCGGTCAGGGTTTGAAAGTGCTTGATAGTTCCGTGGAGAATTTTGCTACAGGAGCTTGGCAAGCATTAGGAAGTGCATTGAAAGGGGGTACTGATCTAGTGCACAAGATTGAGAATTCTGCTGTGAACCTTGCGGGATCTATCCAGCAACGTGGAGGTGCTGGTTCTACCACGCCATCCTTATTAGAGACTGGAAAAGCTTTTACATCAAAGGGAATGCAAGTGCTTGAGCTTGTAGGAAAGGAAACTTTGGATCTACTGATCACAGAGACTGGTATAGAAGTTGATAAGGATTCTACACTTTCTGAAAAGGAAACTGAAGAGGATCAGTTATATGAGGAAGTGGGATTTGATCGGTGTTTCTACATATATGGAGGTCCAGAACAGTTGGAGGAACTGGAGGCTTTGTCCAGCCATTATGCGCTATTATTTAATCGGAGAAAAGGAAAATTGTCATCAGAACAGAAGTCTATTTATGATGGAAAGCTTAAACAGGTGCAACAGATTTTCAGTCTGAATATTGAGTTGGATGGAAGTGGTGCAGAGTCGGACAAAGGCAAAAAGAAAAAGACCGGAGATGATGGAAATATCAATGAGATGAAGAGTTTGCATGATTCAAGCGTCAGCAAGGCTGCTGATATGGCGGCTGGGTTCACAAGTGCCTTAGCAGGACAAGCTGTGAACGATATAATTCAAAGAACAACTGGTAGATTAGAGAGTCTTCACTCAGAGGGGGTTCATGCTGTCAGAATTGTGCTGTTCAGCGGTATCTCAACTGTTGATGCTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF719

Protein Analysis

392

Amino Acids

42.08

Weight (kDa)

4.63

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7798 PF25074 243 - 378 6.4e-70 Domain of unknown function (DUF7798)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 25
AccII CGCG 1 cut(s) 100
AciI CCGC 9 cut(s) 23, 100, 114, 168, 184, 191, 518, 1028, 1149
AclWI GGATC 3 cut(s) 529, 645, 717
AcsI RAATTY 2 cut(s) 424, 499
AcuI CTGAAG 2 cut(s) 105, 723
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 74, 190
AgsI TTSAA 4 cut(s) 400, 466, 1002, 1079
AjuI GAANNNNNNNTTGG 2 cut(s) 893, 925
AluBI AGCT 8 cut(s) 83, 239, 368, 440, 584, 616, 871, 1061
AluI AGCT 8 cut(s) 83, 239, 368, 440, 584, 616, 871, 1061
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 566, 647
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 3 cut(s) 529, 645, 717
AlwNI CAGNNNCTG 1 cut(s) 656
ApaLI GTGCAC 1 cut(s) 485
ApeKI GCWGC 6 cut(s) 80, 194, 212, 239, 344, 1016
ApoI RAATTY 2 cut(s) 424, 499
AspLEI GCGC 1 cut(s) 808
AspS9I GGNCC 2 cut(s) 41, 761
AvaII GGWCC 2 cut(s) 41, 761
BaeGI GKGCMC 1 cut(s) 489
BbsI GAAGAC 3 cut(s) 18, 139, 1097
Bbv12I GWGCWC 1 cut(s) 489
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 6 cut(s) 92, 181, 224, 226, 331, 1003
BccI CCATC 5 cut(s) 114, 568, 857, 908, 959
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 2 cut(s) 566, 647
BfaI CTAG 2 cut(s) 369, 482
BfmI CTRYAG 1 cut(s) 432
BglII AGATCT 2 cut(s) 154, 177
Bme18I GGWCC 2 cut(s) 41, 761
BmgT120I GGNCC 2 cut(s) 41, 761
BmsI GCATC 2 cut(s) 271, 1156
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 3 cut(s) 18, 139, 1097
BpmI CTGGAG 2 cut(s) 143, 803
Bpu10I CCTNAGC 2 cut(s) 243, 1048
BpuEI CTTGAG 2 cut(s) 11, 632
BsaJI CCNNGG 2 cut(s) 264, 417
BsaWI WCCGGW 1 cut(s) 956
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 190
Bse1I ACTGG 4 cut(s) 580, 661, 786, 1093
Bse3DI GCAATG 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 264, 417
BseGI GGATG 4 cut(s) 125, 286, 560, 919
BseLI CCNNNNNNNGG 2 cut(s) 74, 190
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 6 cut(s) 165, 218, 234, 264, 300, 1125
BseNI ACTGG 4 cut(s) 580, 661, 786, 1093
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 6 cut(s) 92, 181, 224, 226, 331, 1003
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 945
Bsh1236I CGCG 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 1 cut(s) 957
BslI CCNNNNNNNGG 2 cut(s) 74, 190
BsmAI GTCTC 2 cut(s) 566, 647
BsmI GAATGC 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
BspACI CCGC 9 cut(s) 23, 100, 114, 168, 184, 191, 518, 1028, 1149
BspCNI CTCAG 6 cut(s) 164, 217, 235, 263, 301, 1124
BspFNI CGCG 1 cut(s) 100
BspPI GGATC 3 cut(s) 529, 645, 717
BspQI GCTCTTC 1 cut(s) 212
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 193
BsrI ACTGG 4 cut(s) 580, 661, 786, 1093
BssECI CCNNGG 2 cut(s) 264, 417
BssMI GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
BssT1I CCWWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 771, 1162
Bst6I CTCTTC 6 cut(s) 48, 168, 212, 249, 699, 980
BstC8I GCNNGC 3 cut(s) 102, 237, 447
BstDEI CTNAG 7 cut(s) 151, 204, 243, 250, 309, 1048, 1111
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 4 cut(s) 125, 286, 560, 919
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 808
BstKTI GATC 8 cut(s) 157, 180, 481, 524, 640, 648, 712, 739
BstMAI GTCTC 2 cut(s) 566, 647
BstMBI GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
BstMWI GCNNNNNNNGC 8 cut(s) 86, 200, 209, 331, 437, 446, 1025, 1058
BstNSI RCATGY 1 cut(s) 235
BstPAI GACNNNNGTC 1 cut(s) 39
BstSFI CTRYAG 1 cut(s) 432
BstSLI GKGCMC 1 cut(s) 489
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 6 cut(s) 92, 181, 224, 226, 331, 1003
BstV2I GAAGAC 3 cut(s) 18, 139, 1097
BstX2I RGATCY 4 cut(s) 154, 177, 521, 637
BstXI CCANNNNNNTGG 1 cut(s) 536
BstYI RGATCY 4 cut(s) 154, 177, 521, 637
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 4 cut(s) 125, 286, 560, 919
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 3 cut(s) 102, 237, 447
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 1 cut(s) 808
Cfr13I GGNCC 2 cut(s) 41, 761
CseI GACGC 1 cut(s) 994
Csp6I GTAC 1 cut(s) 474
CviAII CATG 4 cut(s) 232, 326, 995, 1124
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 7 cut(s) 151, 204, 243, 250, 309, 1048, 1111
DpnI GATC 8 cut(s) 156, 179, 480, 523, 639, 647, 711, 738
DpnII GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
Eam1104I CTCTTC 6 cut(s) 48, 168, 212, 249, 699, 980
EarI CTCTTC 6 cut(s) 48, 168, 212, 249, 699, 980
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 264
Eco47I GGWCC 2 cut(s) 41, 761
Eco57I CTGAAG 2 cut(s) 105, 723
EcoO109I RGGNCCY 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 4 cut(s) 235, 329, 998, 1127
FatI CATG 4 cut(s) 231, 325, 994, 1123
FauI CCCGC 3 cut(s) 93, 191, 511
FbaI TGATCA 1 cut(s) 645
FokI GGATG 4 cut(s) 132, 293, 547, 926
FspBI CTAG 2 cut(s) 369, 482
GlaI GCGC 1 cut(s) 807
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 2 cut(s) 143, 803
HapII CCGG 1 cut(s) 957
HgaI GACGC 1 cut(s) 994
HhaI GCGC 1 cut(s) 808
Hin1II CATG 4 cut(s) 235, 329, 998, 1127
Hin6I GCGC 1 cut(s) 806
HinP1I GCGC 1 cut(s) 806
HindIII AAGCTT 2 cut(s) 582, 869
HinfI GANTC 6 cut(s) 248, 377, 677, 929, 998, 1102
HpaII CCGG 1 cut(s) 957
Hpy166II GTNNAC 4 cut(s) 487, 511, 1038, 1066
Hpy188III TCNNGA 3 cut(s) 160, 350, 764
Hpy8I GTNNAC 4 cut(s) 487, 511, 1038, 1066
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 2 cut(s) 771, 1162
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 7 cut(s) 235, 461, 487, 604, 883, 926, 994
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 200, 209, 331, 437, 446, 1025, 1058
HpyF3I CTNAG 7 cut(s) 151, 204, 243, 250, 309, 1048, 1111
HpySE526I ACGT 1 cut(s) 535
Hsp92II CATG 4 cut(s) 235, 329, 998, 1127
HspAI GCGC 1 cut(s) 806
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 2 cut(s) 77, 437
Lsp1109I GCAGC 6 cut(s) 92, 181, 224, 226, 331, 1003
LweI GCATC 2 cut(s) 271, 1156
MaeI CTAG 2 cut(s) 369, 482
MaeII ACGT 1 cut(s) 535
MalI GATC 8 cut(s) 156, 179, 480, 523, 639, 647, 711, 738
MbiI CCGCTC 1 cut(s) 25
MboI GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
MflI RGATCY 4 cut(s) 154, 177, 521, 637
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 6 cut(s) 356, 424, 499, 833, 1074, 1134
MlyI GAGTC 3 cut(s) 242, 938, 1111
MmeI TCCRAC 3 cut(s) 753, 891, 912
MseI TTAA 2 cut(s) 816, 873
MspA1I CMGCKG 2 cut(s) 83, 1149
MspI CCGG 1 cut(s) 957
Mva1269I GAATGC 1 cut(s) 606
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 8 cut(s) 86, 200, 209, 331, 437, 446, 1025, 1058
NdeII GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
NlaIII CATG 4 cut(s) 235, 329, 998, 1127
NspI RCATGY 1 cut(s) 235
PciSI GCTCTTC 1 cut(s) 212
PctI GAATGC 1 cut(s) 606
PfeI GAWTC 3 cut(s) 377, 677, 998
PleI GAGTC 3 cut(s) 242, 937, 1110
PpsI GAGTC 3 cut(s) 242, 937, 1110
PpuMI RGGWCCY 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 1031
PspPI GGNCC 2 cut(s) 41, 761
PspPPI RGGWCCY 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 4 cut(s) 154, 177, 521, 637
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 816, 873
Sau3AI GATC 8 cut(s) 154, 177, 478, 521, 637, 645, 709, 736
Sau96I GGNCC 2 cut(s) 41, 761
SchI GAGTC 3 cut(s) 242, 938, 1111
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 2 cut(s) 271, 1156
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 2 cut(s) 41, 761
SmlI CTYRAG 2 cut(s) 26, 611
SmoI CTYRAG 2 cut(s) 26, 611
Sse9I AATT 6 cut(s) 356, 424, 499, 833, 1074, 1134
SsiI CCGC 9 cut(s) 23, 100, 114, 168, 184, 191, 518, 1028, 1149
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 369, 482
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 771, 1162
TaiI ACGT 1 cut(s) 538
TaqII GACCGA 1 cut(s) 377
TasI AATT 6 cut(s) 356, 424, 499, 833, 1074, 1134
TauI GCSGC 4 cut(s) 25, 170, 194, 1031
TfiI GAWTC 3 cut(s) 377, 677, 998
Tru1I TTAA 2 cut(s) 816, 873
Tru9I TTAA 2 cut(s) 816, 873
TscAI CASTG 1 cut(s) 313
TseI GCWGC 6 cut(s) 80, 194, 212, 239, 344, 1016
TspDTI ATGAA 3 cut(s) 342, 998, 1112
TspGWI ACGGA 2 cut(s) 133, 406
TspRI CASTG 1 cut(s) 313
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 41, 761
XapI RAATTY 2 cut(s) 424, 499
XceI RCATGY 1 cut(s) 235
XspI CTAG 2 cut(s) 369, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.