pycom10g08550

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004443
Physical Location & Seq
Reverse (-)
11268203 .. 11269703
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
ATGACCAATTTGAATATAAAGGGAAGTGATGATACCTTCGTTTCAACTGCTGATCGGGTGAAATCCTTCAAAACTGATGAAGATCCTTCCTTTGTGGAACTTTTATTCCAGTATGGCCGGTATCTGCTTATTTCTTGTTCACGGGTTGGAACTCAAGTTGCAAACCTGCAGGGTATATGGAGCAAGGATCTTTACCCTCCATGGGATGGCGCTCAGCATCTAAACATAAATCTTCAAATGAATTACTGGCCATCCCTTCCTTGTAACCTAAGAGAATGCCAGGAGCCCTTATTTGATTACACATCTTCTTTATCCATCAACGGGAGTAAAACGGCTAAGGTGAACTATGAAGCCAGTGGTTGGGTTGTGCATCAAGTGTCTGACATATGGGCGAAAACATCACCCGATCGAGGTCAAGCTGTGTGGGCTTTATGGCCAATGGGAGGAGCCTGGATTTGTACTCATCTATGGGAACATTTTACTTATACGATGGACAAAGATTTTCTAAAAAACAAGGCATATCCTTTGTTGGAAGGATGTACATCATTTCTGCTAGATTGGTTGATTGAAGGCCGTGGAGGGTATCTTGAAACCAACCCGTCAACTTCTCCAGAGCACATGTTTATTGCTCCTGATGGTAAGCAGGCAAGCGTGAGCTACTCATCAACCATGGACATATCAATCATCAAAGAAGTTTTCTCTGCAATACTTTCTGCGGCTGAGGTTTTATTTTGGGAAAAACACAGGATGCTCTTGTTGGAAAAGTTCGTGAGGCTCAATCTCGGCTGCCACCCACAAAAATTGCTAAAGATGGTTCTATCATGGAATGGGCACAAGATTTTGAGGACCCAGATGTGCACCATCGACATGTATCACACCTGTTTGGCTTGTATCCAGGACATACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0004560 GO:0005575 GO:0005576 GO:0015928 GO:0016787 GO:0016798 GO:0047513 GO:0048046
KEGG Pathways
Metabolic & Signaling
ko00511 map00511
Pfam Domains
Protein Families
Glyco_hyd_65N_2

Protein Analysis

303

Amino Acids

34.5

Weight (kDa)

5.98

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_95_cat PF22124 3 - 245 6.4e-99 Glycosyl hydrolase family 95 catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AccB7I CCANNNNNTGG 2 cut(s) 206, 360
AciI CCGC 1 cut(s) 716
AclWI GGATC 2 cut(s) 77, 195
AcoI YGGCCR 3 cut(s) 115, 248, 434
AfaI GTAC 2 cut(s) 460, 541
AfiI CCNNNNNNNGG 6 cut(s) 202, 206, 321, 360, 410, 443
AflIII ACRYGT 2 cut(s) 618, 867
AgsI TTSAA 6 cut(s) 13, 45, 70, 236, 569, 590
AjnI CCWGG 3 cut(s) 279, 449, 894
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 2 cut(s) 419, 657
AluI AGCT 2 cut(s) 419, 657
Alw21I GWGCWC 2 cut(s) 618, 860
Alw44I GTGCAC 1 cut(s) 856
AlwI GGATC 2 cut(s) 77, 195
AoxI GGCC 4 cut(s) 115, 248, 434, 571
ApaLI GTGCAC 1 cut(s) 856
ApeKI GCWGC 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 846
AsuHPI GGTGA 3 cut(s) 70, 352, 393
AvaII GGWCC 1 cut(s) 846
BaeGI GKGCMC 2 cut(s) 834, 860
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BalI TGGCCA 2 cut(s) 250, 436
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 16, 48
Bbv12I GWGCWC 2 cut(s) 618, 860
BbvCI CCTCAGC 1 cut(s) 720
BbvI GCAGC 1 cut(s) 773
BccI CCATC 7 cut(s) 200, 259, 323, 484, 629, 805, 869
BceAI ACGGC 2 cut(s) 348, 558
BciT130I CCWGG 3 cut(s) 281, 451, 896
BciVI GTATCC 1 cut(s) 902
BfaI CTAG 1 cut(s) 554
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 174
BfuI GTATCC 1 cut(s) 902
BisI GCNGC 2 cut(s) 717, 787
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 2 cut(s) 718, 788
Bme1390I CCNGG 3 cut(s) 281, 451, 896
Bme18I GGWCC 1 cut(s) 846
BmgT120I GGNCC 1 cut(s) 846
BmiI GGNNCC 3 cut(s) 285, 448, 848
BmrFI CCNGG 3 cut(s) 281, 451, 896
BmsI GCATC 3 cut(s) 226, 379, 738
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 2 cut(s) 336, 720
Bpu1102I GCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 138
BsaBI GATNNNNATC 2 cut(s) 81, 541
BsaJI CCNNGG 3 cut(s) 200, 574, 669
Bsc4I CCNNNNNNNGG 6 cut(s) 202, 206, 321, 360, 410, 443
Bse118I RCCGGY 1 cut(s) 117
Bse1I ACTGG 3 cut(s) 109, 251, 354
Bse8I GATNNNNATC 2 cut(s) 81, 541
BseBI CCWGG 3 cut(s) 281, 451, 896
BseDI CCNNGG 3 cut(s) 200, 574, 669
BseGI GGATG 4 cut(s) 211, 251, 542, 753
BseJI GATNNNNATC 2 cut(s) 81, 541
BseLI CCNNNNNNNGG 6 cut(s) 202, 206, 321, 360, 410, 443
BseMII CTCAG 2 cut(s) 227, 711
BseNI ACTGG 3 cut(s) 109, 251, 354
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 2 cut(s) 834, 860
BseXI GCAGC 1 cut(s) 773
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 4 cut(s) 117, 250, 436, 573
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 618, 860
BsiSI CCGG 1 cut(s) 118
BslI CCNNNNNNNGG 6 cut(s) 202, 206, 321, 360, 410, 443
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 4 cut(s) 117, 250, 436, 573
Bsp1286I GDGCHC 4 cut(s) 288, 618, 834, 860
Bsp1407I TGTACA 1 cut(s) 539
Bsp143I GATC 4 cut(s) 52, 82, 187, 406
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 2 cut(s) 200, 669
BspACI CCGC 1 cut(s) 716
BspANI GGCC 4 cut(s) 117, 250, 436, 573
BspCNI CTCAG 2 cut(s) 226, 712
BspLI GGNNCC 3 cut(s) 285, 448, 848
BspMAI CTGCAG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 77, 195
BsrFI RCCGGY 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 539
BsrI ACTGG 3 cut(s) 109, 251, 354
BssAI RCCGGY 1 cut(s) 117
BssECI CCNNGG 3 cut(s) 200, 574, 669
BssMI GATC 4 cut(s) 52, 82, 187, 406
BssT1I CCWWGG 2 cut(s) 200, 669
Bst2UI CCWGG 3 cut(s) 281, 451, 896
BstAUI TGTACA 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 645, 649
BstDEI CTNAG 4 cut(s) 213, 269, 336, 720
BstDSI CCRYGG 3 cut(s) 200, 574, 669
BstF5I GGATG 4 cut(s) 211, 251, 542, 753
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 4 cut(s) 55, 85, 190, 409
BstMBI GATC 4 cut(s) 52, 82, 187, 406
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 3 cut(s) 281, 451, 896
BstNSI RCATGY 2 cut(s) 622, 871
BstSCI CCNGG 3 cut(s) 279, 449, 894
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 2 cut(s) 834, 860
BstV1I GCAGC 1 cut(s) 773
BstX2I RGATCY 2 cut(s) 82, 187
BstYI RGATCY 2 cut(s) 82, 187
BsuI GTATCC 1 cut(s) 902
BsuRI GGCC 4 cut(s) 117, 250, 436, 573
BtgI CCRYGG 3 cut(s) 200, 574, 669
BtsCI GGATG 4 cut(s) 211, 251, 542, 753
BtsIMutI CAGTG 1 cut(s) 361
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 645, 649
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 846
Csp6I GTAC 2 cut(s) 459, 540
CspCI CAANNNNNGTGG 4 cut(s) 404, 439, 783, 818
CviAII CATG 5 cut(s) 201, 619, 670, 822, 868
CviQI GTAC 2 cut(s) 459, 540
DdeI CTNAG 4 cut(s) 213, 269, 336, 720
DpnI GATC 4 cut(s) 54, 84, 189, 408
DpnII GATC 4 cut(s) 52, 82, 187, 406
EaeI YGGCCR 3 cut(s) 115, 248, 434
Eco130I CCWWGG 2 cut(s) 200, 669
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 846
EcoO109I RGGNCCY 1 cut(s) 846
EcoRII CCWGG 3 cut(s) 279, 449, 894
EcoT14I CCWWGG 2 cut(s) 200, 669
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 2 cut(s) 200, 669
FaeI CATG 5 cut(s) 204, 622, 673, 825, 871
FatI CATG 5 cut(s) 200, 618, 669, 821, 867
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 2 cut(s) 717, 787
FokI GGATG 4 cut(s) 218, 238, 549, 760
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 717, 787
FspBI CTAG 1 cut(s) 554
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 717, 787
GsuI CTGGAG 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 4 cut(s) 117, 250, 436, 573
HapII CCGG 1 cut(s) 118
HhaI GCGC 1 cut(s) 212
Hin1II CATG 5 cut(s) 204, 622, 673, 825, 871
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 603
HindII GTYRAC 1 cut(s) 603
HpaII CCGG 1 cut(s) 118
HphI GGTGA 3 cut(s) 70, 352, 393
Hpy166II GTNNAC 4 cut(s) 140, 343, 603, 858
Hpy188I TCNGA 1 cut(s) 382
Hpy188III TCNNGA 4 cut(s) 587, 611, 632, 769
Hpy8I GTNNAC 4 cut(s) 140, 343, 603, 858
HpyAV CCTTC 6 cut(s) 46, 76, 96, 266, 527, 563
HpyCH4V TGCA 5 cut(s) 161, 169, 370, 704, 858
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 4 cut(s) 213, 269, 336, 720
Hsp92II CATG 5 cut(s) 204, 622, 673, 825, 871
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 4 cut(s) 52, 82, 187, 406
LmnI GCTCC 4 cut(s) 180, 283, 446, 634
Lsp1109I GCAGC 1 cut(s) 773
LweI GCATC 3 cut(s) 226, 379, 738
MaeI CTAG 1 cut(s) 554
MaeIII GTNAC 1 cut(s) 263
MalI GATC 4 cut(s) 54, 84, 189, 408
MboI GATC 4 cut(s) 52, 82, 187, 406
MboII GAAGA 3 cut(s) 92, 224, 297
MflI RGATCY 2 cut(s) 82, 187
MhlI GDGCHC 4 cut(s) 288, 618, 834, 860
MlsI TGGCCA 2 cut(s) 250, 436
MluCI AATT 3 cut(s) 7, 241, 800
MluNI TGGCCA 2 cut(s) 250, 436
MmeI TCCRAC 3 cut(s) 127, 510, 738
MnlI CCTC 7 cut(s) 207, 404, 437, 572, 715, 765, 837
Mox20I TGGCCA 2 cut(s) 250, 436
MroXI GAANNNNTTC 1 cut(s) 65
MscI TGGCCA 2 cut(s) 250, 436
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 2 cut(s) 250, 436
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 3 cut(s) 281, 451, 896
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 3 cut(s) 281, 451, 896
MwoI GCNNNNNNNGC 1 cut(s) 425
NcoI CCATGG 2 cut(s) 200, 669
NdeI CATATG 1 cut(s) 386
NdeII GATC 4 cut(s) 52, 82, 187, 406
NlaIII CATG 5 cut(s) 204, 622, 673, 825, 871
NlaIV GGNNCC 3 cut(s) 285, 448, 848
NmeAIII GCCGAG 1 cut(s) 762
NspI RCATGY 2 cut(s) 622, 871
PciI ACATGT 2 cut(s) 618, 867
PctI GAATGC 1 cut(s) 281
PdmI GAANNNNTTC 1 cut(s) 65
PflMI CCANNNNNTGG 2 cut(s) 206, 360
PfoI TCCNGGA 1 cut(s) 894
PkrI GCNGC 2 cut(s) 718, 788
Ple19I CGATCG 1 cut(s) 409
PpuMI RGGWCCY 1 cut(s) 846
PscI ACATGT 2 cut(s) 618, 867
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 3 cut(s) 279, 449, 894
PspGI CCWGG 3 cut(s) 279, 449, 894
PspN4I GGNNCC 3 cut(s) 285, 448, 848
PspPI GGNCC 1 cut(s) 846
PspPPI RGGWCCY 1 cut(s) 846
PstI CTGCAG 1 cut(s) 171
PsuI RGATCY 2 cut(s) 82, 187
PvuI CGATCG 1 cut(s) 409
RsaI GTAC 2 cut(s) 460, 541
RsaNI GTAC 2 cut(s) 459, 540
RseI CAYNNNNRTG 1 cut(s) 866
SatI GCNGC 2 cut(s) 717, 787
Sau3AI GATC 4 cut(s) 52, 82, 187, 406
Sau96I GGNCC 1 cut(s) 846
SbfI CCTGCAGG 1 cut(s) 171
ScrFI CCNGG 3 cut(s) 281, 451, 896
SdaI CCTGCAGG 1 cut(s) 171
SduI GDGCHC 4 cut(s) 288, 618, 834, 860
SetI ASST 9 cut(s) 38, 168, 270, 342, 415, 421, 659, 726, 881
SfaNI GCATC 3 cut(s) 226, 379, 738
SfcI CTRYAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 846
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
Sse8387I CCTGCAGG 1 cut(s) 171
Sse9I AATT 3 cut(s) 7, 241, 800
SsiI CCGC 1 cut(s) 716
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 3 cut(s) 279, 449, 894
StyI CCWWGG 2 cut(s) 200, 669
TaqI TCGA 2 cut(s) 409, 864
TasI AATT 3 cut(s) 7, 241, 800
TatI WGTACW 2 cut(s) 458, 539
TauI GCSGC 1 cut(s) 719
TscAI CASTG 1 cut(s) 361
TseI GCWGC 1 cut(s) 786
TspDTI ATGAA 3 cut(s) 93, 254, 363
TspRI CASTG 1 cut(s) 361
Van91I CCANNNNNTGG 2 cut(s) 206, 360
VneI GTGCAC 1 cut(s) 856
VpaK11BI GGWCC 1 cut(s) 846
XceI RCATGY 2 cut(s) 622, 871
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.