pycom10g08680

SWI complex, BAF60b domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007672
Physical Location & Seq
Forward (+)
11463688 .. 11465570
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGAGGAATAAGGCGGGCTCAGAATACTCACTCTCCTCCACCCTACAAGACTCCCGACTCTCAAAACGCTATCCCTTAAGCGGACCAAACGACATGGCGGTGACCACAGGCCTCATCTCCTCCACCTTCTTCTCCGGCGAGACGTCGTCGTTCGTCAGCCTTCCTTTCTCCTCCTCGTCCTCCTCCCTCAGGTTCGCCCCCGCTAACCTGCGCATGGTGCGCATCCCAACCCTTGCCACTGCCTCGAAGCCGGCCACCACCACCCCCCGCGAGCCCCGCGGCATCATGAAGCCTCGCAAAGTCTCGCTCGAGATGCAAGCCCTTGTGGGTGCCCCTGAGATTTCTCGCACCCAAGCTCTGAAGCAGATTTGGGCCCACATTAAGCAGAACAATCTTCAGGACCCCCAAAACAAGAGGATCATAGTCTGTGATGAGAAGCTGAAGCAGATCTTTGCCGGGAAGGACCGTGTTGGATTTCTTGAGGTTGCTGGATTGATCACCCCCCATTTCCTTTGA

Protein Analysis

172

Amino Acids

18.79

Weight (kDa)

10.32

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 97 - 170 1.3e-28 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
Acc16I TGCGCA 2 cut(s) 212, 221
Acc36I ACCTGC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 329
AccII CGCG 2 cut(s) 270, 279
AciI CCGC 7 cut(s) 14, 81, 98, 201, 268, 277, 279
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 1 cut(s) 252
AcuI CTGAAG 3 cut(s) 380, 380, 461
AcyI GRCGYC 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 80, 189, 214
AflII CTTAAG 1 cut(s) 76
AluBI AGCT 2 cut(s) 356, 439
AluI AGCT 2 cut(s) 356, 439
Alw26I GTCTC 2 cut(s) 134, 307
AlwI GGATC 1 cut(s) 425
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 109, 252, 372
ApaI GGGCCC 1 cut(s) 376
AspLEI GCGC 2 cut(s) 213, 222
AspS9I GGNCC 5 cut(s) 83, 372, 373, 400, 463
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 2 cut(s) 112, 490
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 3 cut(s) 83, 400, 463
AxyI CCTNAGG 1 cut(s) 188
BaeGI GKGCMC 2 cut(s) 334, 376
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 3 cut(s) 20, 276, 376
BclI TGATCA 1 cut(s) 495
BcnI CCSGG 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 134, 307
BfrI CTTAAG 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 216
BglII AGATCT 1 cut(s) 447
BisI GCNGC 1 cut(s) 280
BlsI GCNGC 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 457
Bme18I GGWCC 3 cut(s) 83, 400, 463
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 5 cut(s) 83, 372, 373, 400, 463
BmiI GGNNCC 3 cut(s) 331, 374, 402
BmrFI CCNGG 1 cut(s) 457
BmsI GCATC 3 cut(s) 231, 291, 303
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 457
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 189, 214
Bse118I RCCGGY 1 cut(s) 250
Bse21I CCTNAGG 1 cut(s) 188
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 80, 189, 214
BseMII CTCAG 3 cut(s) 33, 202, 327
BseRI GAGGAG 5 cut(s) 25, 109, 160, 163, 172
BseSI GKGCMC 2 cut(s) 334, 376
Bsh1236I CGCG 2 cut(s) 270, 279
BshFI GGCC 3 cut(s) 111, 254, 374
BshNI GGYRCC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 3 cut(s) 135, 251, 456
BslI CCNNNNNNNGG 3 cut(s) 80, 189, 214
BsmAI GTCTC 2 cut(s) 134, 307
BsmBI CGTCTC 1 cut(s) 134
BsnI GGCC 3 cut(s) 111, 254, 374
BsoBI CYCGRG 1 cut(s) 308
Bsp120I GGGCCC 1 cut(s) 372
Bsp1286I GDGCHC 4 cut(s) 20, 276, 334, 376
Bsp143I GATC 3 cut(s) 417, 447, 495
BspACI CCGC 7 cut(s) 14, 81, 98, 201, 268, 277, 279
BspANI GGCC 3 cut(s) 111, 254, 374
BspCNI CTCAG 3 cut(s) 32, 201, 328
BspFNI CGCG 2 cut(s) 270, 279
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 3 cut(s) 331, 374, 402
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 329
BspTI CTTAAG 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 3 cut(s) 417, 447, 495
BssNI GRCGYC 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 467
BstACI GRCGYC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 76
BstC8I GCNNGC 4 cut(s) 16, 252, 272, 318
BstDEI CTNAG 3 cut(s) 19, 188, 336
BstDSI CCRYGG 1 cut(s) 277
BstEII GGTNACC 1 cut(s) 100
BstENI CCTNNNNNAGG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 2 cut(s) 270, 279
BstHHI GCGC 2 cut(s) 213, 222
BstKTI GATC 3 cut(s) 420, 450, 498
BstMAI GTCTC 2 cut(s) 134, 307
BstMBI GATC 3 cut(s) 417, 447, 495
BstMWI GCNNNNNNNGC 4 cut(s) 217, 219, 276, 313
BstPI GGTNACC 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 455
BstSLI GKGCMC 2 cut(s) 334, 376
BstUI CGCG 2 cut(s) 270, 279
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
Bsu36I CCTNAGG 1 cut(s) 188
BsuRI GGCC 3 cut(s) 111, 254, 374
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 4 cut(s) 16, 252, 272, 318
CciI TCATGA 1 cut(s) 285
CfoI GCGC 2 cut(s) 213, 222
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 5 cut(s) 83, 372, 373, 400, 463
Cfr42I CCGCGG 1 cut(s) 280
CviAII CATG 3 cut(s) 94, 214, 286
DdeI CTNAG 3 cut(s) 19, 188, 336
DpnI GATC 3 cut(s) 419, 449, 497
DpnII GATC 3 cut(s) 417, 447, 495
EaeI YGGCCR 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 20, 276, 376
Eco47I GGWCC 3 cut(s) 83, 400, 463
Eco57I CTGAAG 3 cut(s) 380, 380, 461
Eco81I CCTNAGG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 308
Eco91I GGTNACC 1 cut(s) 100
EcoNI CCTNNNNNAGG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 400
EcoO65I GGTNACC 1 cut(s) 100
EcoT38I GRGCYC 3 cut(s) 20, 276, 376
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 3 cut(s) 97, 217, 289
FaiI YATR 4 cut(s) 95, 215, 287, 422
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FatI CATG 3 cut(s) 93, 213, 285
FauI CCCGC 4 cut(s) 7, 208, 275, 284
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 1 cut(s) 280
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 3 cut(s) 20, 276, 376
Fsp4HI GCNGC 1 cut(s) 280
FspAI RTGCGCAY 1 cut(s) 221
FspI TGCGCA 2 cut(s) 212, 221
GlaI GCGC 2 cut(s) 212, 221
GluI GCNGC 1 cut(s) 280
HaeIII GGCC 3 cut(s) 111, 254, 374
HapII CCGG 3 cut(s) 135, 251, 456
HhaI GCGC 2 cut(s) 213, 222
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 97, 217, 289
Hin6I GCGC 2 cut(s) 211, 220
HinP1I GCGC 2 cut(s) 211, 220
HinfI GANTC 2 cut(s) 50, 57
HpaII CCGG 3 cut(s) 135, 251, 456
HphI GGTGA 2 cut(s) 112, 490
Hpy188I TCNGA 2 cut(s) 22, 360
Hpy188III TCNNGA 5 cut(s) 54, 286, 310, 398, 479
Hpy99I CGWCG 2 cut(s) 148, 151
HpyAV CCTTC 3 cut(s) 136, 170, 454
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 219, 276, 313
HpyF3I CTNAG 3 cut(s) 19, 188, 336
HpySE526I ACGT 1 cut(s) 143
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 97, 217, 289
HspAI GCGC 2 cut(s) 211, 220
KroI GCCGGC 1 cut(s) 250
KroNI GCCGGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 495
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 3 cut(s) 417, 447, 495
LpnPI CCDG 9 cut(s) 93, 148, 175, 221, 264, 348, 383, 469, 474
LweI GCATC 3 cut(s) 231, 291, 303
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 1 cut(s) 100
MalI GATC 3 cut(s) 419, 449, 497
MboI GATC 3 cut(s) 417, 447, 495
MboII GAAGA 2 cut(s) 121, 386
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 4 cut(s) 20, 276, 334, 376
MlyI GAGTC 2 cut(s) 44, 51
MmeI TCCRAC 1 cut(s) 451
MroNI GCCGGC 1 cut(s) 250
MseI TTAA 2 cut(s) 77, 381
MslI CAYNNNNRTG 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 279
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 3 cut(s) 135, 251, 456
MspR9I CCNGG 1 cut(s) 457
MvnI CGCG 2 cut(s) 270, 279
MwoI GCNNNNNNNGC 4 cut(s) 217, 219, 276, 313
NaeI GCCGGC 1 cut(s) 252
NciI CCSGG 1 cut(s) 457
NdeII GATC 3 cut(s) 417, 447, 495
NgoMIV GCCGGC 1 cut(s) 250
NlaIII CATG 3 cut(s) 97, 217, 289
NlaIV GGNNCC 3 cut(s) 331, 374, 402
NmuCI GTSAC 1 cut(s) 100
NsbI TGCGCA 2 cut(s) 212, 221
PaeR7I CTCGAG 1 cut(s) 308
PagI TCATGA 1 cut(s) 285
PceI AGGCCT 1 cut(s) 111
PdiI GCCGGC 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 145
PkrI GCNGC 1 cut(s) 281
PleI GAGTC 2 cut(s) 44, 51
PpsI GAGTC 2 cut(s) 44, 51
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 331, 374, 402
PspOMI GGGCCC 1 cut(s) 372
PspPI GGNCC 5 cut(s) 83, 372, 373, 400, 463
PspPPI RGGWCCY 1 cut(s) 400
PsuI RGATCY 1 cut(s) 447
PsyI GACNNNGTC 1 cut(s) 145
RseI CAYNNNNRTG 1 cut(s) 98
SacII CCGCGG 1 cut(s) 280
SaqAI TTAA 2 cut(s) 77, 381
SatI GCNGC 1 cut(s) 280
Sau3AI GATC 3 cut(s) 417, 447, 495
Sau96I GGNCC 5 cut(s) 83, 372, 373, 400, 463
SchI GAGTC 2 cut(s) 44, 51
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 4 cut(s) 20, 276, 334, 376
SetI ASST 7 cut(s) 128, 146, 194, 210, 358, 441, 486
SfaNI GCATC 3 cut(s) 231, 291, 303
Sfr274I CTCGAG 1 cut(s) 308
Sfr303I CCGCGG 1 cut(s) 280
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 3 cut(s) 83, 400, 463
SlaI CTCGAG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 76, 308, 479
SmoI CTYRAG 3 cut(s) 76, 308, 479
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 7 cut(s) 14, 81, 98, 201, 268, 277, 279
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 1 cut(s) 467
TaiI ACGT 1 cut(s) 146
TaqI TCGA 2 cut(s) 245, 309
TauI GCSGC 1 cut(s) 282
Tru1I TTAA 2 cut(s) 77, 381
Tru9I TTAA 2 cut(s) 77, 381
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 1 cut(s) 244
Tth111I GACNNNGTC 1 cut(s) 145
Vha464I CTTAAG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 83, 400, 463
XagI CCTNNNNNAGG 1 cut(s) 187
XhoI CTCGAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.