Rorug05G0089400

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007672
Physical Location & Seq
Reverse (-)
7683130 .. 7685962
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0089400.1

Sequence Viewer

Length: 1551 bp
ATGGAACAAGATACCCAACTCCCAATCCCAAGCAACGATCAAAACGTCGACGTTTTCTTCGACGCCCTCGACGATTTCCCTCCCTCCTCCGCCGACGACCAACCCGATCAATCCACGTCATCCTCCACCCTCGATGTCCCCGATTCCAAACCCTCTCCGATCTCCCACCTCCGTCTCCGGCCCTCCCGCCGAGAACTCTCCGGCGACGACTCCAAGACCTCCGCTTTACACTCCTCCCAAACCGACCCAATCAACGACGCCAGGCTCAATTACCGAGGCAGAAAGTTCTTACTTCGCCGGAATTTGAAGCCGCCGGAGTCGATTCAGGACCCGGTCGGACCGGTGCCAGCTCCGACCGACCAGAAAGACGAGGGCTCGACTGTAACTACCGCCGCCGACGATGACCGAGCCGGTGACTCGGCCGACTCGGCCATGCAACTCGGTGACTCTTCTTTTAACCCTCTTGTGTTCATAGCTGGATTGATAATCAAAGCAATTGGGCTCCAAATTAATCTGATCATTAGCATTGTGACATTCCCAGCTTGGATTTTGTATAATTCTTACATGCTTTTGGTTGACCCTTTACAAATTGTGGGTAGAGGGAGAGAGTTTTTGAAGGCAAAGTTGGTAAATTTATCGGCATTGGCTGCCGGAGCGGTTAGTCCTTTGGTTGGTGAGTGGGTGAAGGACAAGGACTCGGTTTGGAAGGTGGCATTGCGATTCGGGTGGGGTTTGCTATGGGCGTGTTATGTAGGTTTCTTGTTGTGTGGTCTTTTGGTGTCGTCGCTTGTGTTTAGTGGGGTTTTTATGAGGTACATTGTGGTCGAGCCGCTGCAGATGAAGGAAATGTTGAACTTTGATTACACAAAGCATAGTCCGGTGGCCTATGTGCCGGTGGTGTCGTGTGCAGGGGTCGGTTGTGGTGTGGATTGTAAAGAGAAGGTTCGGGATGGGGGGATTTTCGGGTATAGGGTTATACCTCCTGGTCACAAATTGGCGGCTAGTGTTAAGTTTGTGTTGCCTGAATCGGAGTACAACAGGAATCTTGGGATCTTTCAGGTCAGGGTACAGTTCCTGTCTGCTGAAGGCAAAACCCTTGCCAGTTTGAGCCATCCATGCATGTTATATTTTAAAAGTGAGCCAATCCGCCTTCTATTGACGTTCATAAAGATTGCTCCTCTTATATCTGGCTACATTTCAGAGTCCCAAACTCTGAATCTGAAGTTCCGAGGGTTTGTGGAAGGTGATATTCCTACTGCCTGCTTGAAAGTGACAATCGAACAACGTGCAGAATACAATCCTGGCGCTGGTATTCCCGAAATATACGATTCATCCATGACCCTTGAATCGGAACTTCCCCTGTTTAAAAGGATTATATGGAACTGGAGGAGAACTTTATTTGTTTGGATGAGTATAATGATGTTCATGATGGAGATGCTCTTTACTCTTGTCTGTTGTAGACCTCTAGTTATTCCGAGAGCAAGGCCAAGGGCTGCTTCTGTCAGCACCAGTCCCACTCAAACTCAAAGCAGCCCGCCGGTACGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

516

Amino Acids

57.15

Weight (kDa)

6.56

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seipin PF06775 257 - 475 5.1e-50 Putative adipose-regulatory protein (Seipin)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 656
AccI GTMKAC 2 cut(s) 48, 1459
AclWI GGATC 1 cut(s) 1058
AcoI YGGCCR 2 cut(s) 420, 429
AcsI RAATTY 2 cut(s) 301, 631
AcuI CTGAAG 2 cut(s) 1104, 1241
AcyI GRCGYC 2 cut(s) 63, 258
AfaI GTAC 4 cut(s) 815, 1034, 1068, 1542
AfiI CCNNNNNNNGG 3 cut(s) 671, 1307, 1348
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 5 cut(s) 307, 616, 853, 1267, 1346
AjiI CACGTC 1 cut(s) 117
AjnI CCWGG 3 cut(s) 260, 982, 1300
AjuI GAANNNNNNNTTGG 4 cut(s) 608, 640, 1480, 1512
AluBI AGCT 3 cut(s) 350, 476, 542
AluI AGCT 3 cut(s) 350, 476, 542
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 1058
AlwNI CAGNNNCTG 1 cut(s) 1075
AoxI GGCC 5 cut(s) 179, 420, 429, 882, 1484
ApeKI GCWGC 4 cut(s) 647, 832, 1493, 1530
ApoI RAATTY 2 cut(s) 301, 631
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
AseI ATTAAT 1 cut(s) 510
AsiGI ACCGGT 1 cut(s) 340
AspLEI GCGC 1 cut(s) 1307
AspS9I GGNCC 3 cut(s) 180, 328, 338
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 5 cut(s) 425, 455, 686, 694, 1256
AvaII GGWCC 2 cut(s) 328, 338
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 2 cut(s) 377, 504
BbvI GCAGC 4 cut(s) 634, 819, 1480, 1542
BccI CCATC 3 cut(s) 944, 1119, 1423
BcgI CGANNNNNNTGC 2 cut(s) 1268, 1302
BciT130I CCWGG 3 cut(s) 262, 984, 1302
BclI TGATCA 1 cut(s) 516
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 3 cut(s) 1002, 1466, 1549
BfmI CTRYAG 1 cut(s) 833
BfoI RGCGCY 1 cut(s) 1308
BglI GCCNNNNNGGC 1 cut(s) 428
BisI GCNGC 8 cut(s) 311, 393, 648, 830, 833, 999, 1494, 1531
BlsI GCNGC 8 cut(s) 312, 394, 649, 831, 834, 1000, 1495, 1532
Bme1390I CCNGG 4 cut(s) 262, 332, 984, 1302
Bme18I GGWCC 2 cut(s) 328, 338
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 180, 328, 338
BmiI GGNNCC 3 cut(s) 330, 345, 503
BmrFI CCNGG 4 cut(s) 262, 332, 984, 1302
BmsI GCATC 1 cut(s) 1425
BpmI CTGGAG 1 cut(s) 1405
BpuMI CCSGG 1 cut(s) 332
BsaHI GRCGYC 2 cut(s) 63, 258
BsaJI CCNNGG 3 cut(s) 274, 1228, 1487
BsaWI WCCGGW 2 cut(s) 340, 877
Bsc4I CCNNNNNNNGG 3 cut(s) 671, 1307, 1348
Bse118I RCCGGY 4 cut(s) 340, 410, 892, 1537
Bse1I ACTGG 3 cut(s) 1101, 1388, 1509
Bse3DI GCAATG 1 cut(s) 713
BseBI CCWGG 3 cut(s) 262, 984, 1302
BseDI CCNNGG 3 cut(s) 274, 1228, 1487
BseGI GGATG 5 cut(s) 119, 955, 1111, 1331, 1413
BseLI CCNNNNNNNGG 3 cut(s) 671, 1307, 1348
BseMI GCAATG 1 cut(s) 713
BseNI ACTGG 3 cut(s) 1101, 1388, 1509
BseRI GAGGAG 4 cut(s) 76, 223, 1167, 1402
BseX3I CGGCCG 1 cut(s) 420
BseXI GCAGC 4 cut(s) 634, 819, 1480, 1542
BseYI CCCAGC 1 cut(s) 538
BsgI GTGCAG 2 cut(s) 927, 1308
Bsh1285I CGRYCG 3 cut(s) 336, 357, 423
BshFI GGCC 5 cut(s) 181, 422, 431, 884, 1486
BshNI GGYRCC 1 cut(s) 343
BshTI ACCGGT 1 cut(s) 340
BsiEI CGRYCG 3 cut(s) 336, 357, 423
BslFI GGGAC 3 cut(s) 122, 1189, 1497
BslI CCNNNNNNNGG 3 cut(s) 671, 1307, 1348
BsmAI GTCTC 1 cut(s) 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 3 cut(s) 122, 1189, 1497
BsnI GGCC 5 cut(s) 181, 422, 431, 884, 1486
Bsp1286I GDGCHC 2 cut(s) 377, 504
Bsp143I GATC 5 cut(s) 37, 106, 159, 516, 1050
BspANI GGCC 5 cut(s) 181, 422, 431, 884, 1486
BspHI TCATGA 1 cut(s) 1425
BspLI GGNNCC 3 cut(s) 330, 345, 503
BspMAI CTGCAG 1 cut(s) 837
BspPI GGATC 1 cut(s) 1058
BspT107I GGYRCC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 713
BsrFI RCCGGY 4 cut(s) 340, 410, 892, 1537
BsrI ACTGG 3 cut(s) 1101, 1388, 1509
BssAI RCCGGY 4 cut(s) 340, 410, 892, 1537
BssECI CCNNGG 3 cut(s) 274, 1228, 1487
BssMI GATC 5 cut(s) 37, 106, 159, 516, 1050
BssNI GRCGYC 2 cut(s) 63, 258
BssT1I CCWWGG 1 cut(s) 1487
Bst2UI CCWGG 3 cut(s) 262, 984, 1302
Bst4CI ACNGT 2 cut(s) 382, 1071
Bst6I CTCTTC 1 cut(s) 454
BstACI GRCGYC 2 cut(s) 63, 258
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 348, 1261, 1535
BstF5I GGATG 5 cut(s) 119, 955, 1111, 1331, 1413
BstH2I RGCGCY 1 cut(s) 1308
BstHHI GCGC 1 cut(s) 1307
BstKTI GATC 5 cut(s) 40, 109, 162, 519, 1053
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 5 cut(s) 37, 106, 159, 516, 1050
BstMCI CGRYCG 3 cut(s) 336, 357, 423
BstMWI GCNNNNNNNGC 4 cut(s) 428, 647, 653, 1116
BstNI CCWGG 3 cut(s) 262, 984, 1302
BstNSI RCATGY 2 cut(s) 568, 1123
BstSCI CCNGG 4 cut(s) 260, 330, 982, 1300
BstSFI CTRYAG 1 cut(s) 833
BstV1I GCAGC 4 cut(s) 634, 819, 1480, 1542
BstX2I RGATCY 1 cut(s) 1050
BstYI RGATCY 1 cut(s) 1050
BstZI CGGCCG 1 cut(s) 420
BsuRI GGCC 5 cut(s) 181, 422, 431, 884, 1486
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 5 cut(s) 119, 955, 1111, 1331, 1413
Cac8I GCNNGC 3 cut(s) 348, 1261, 1535
CaiI CAGNNNCTG 1 cut(s) 1075
CciI TCATGA 1 cut(s) 1425
CfoI GCGC 1 cut(s) 1307
Cfr10I RCCGGY 4 cut(s) 340, 410, 892, 1537
Cfr13I GGNCC 3 cut(s) 180, 328, 338
CpoI CGGWCCG 1 cut(s) 338
CseI GACGC 2 cut(s) 71, 266
Csp6I GTAC 4 cut(s) 814, 1033, 1067, 1541
CspAI ACCGGT 1 cut(s) 340
CspI CGGWCCG 1 cut(s) 338
CviAII CATG 6 cut(s) 433, 565, 1116, 1120, 1336, 1426
CviQI GTAC 4 cut(s) 814, 1033, 1067, 1541
DpnI GATC 5 cut(s) 39, 108, 161, 518, 1052
DpnII GATC 5 cut(s) 37, 106, 159, 516, 1050
DraI TTTAAA 2 cut(s) 1132, 1366
EaeI YGGCCR 2 cut(s) 420, 429
EagI CGGCCG 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
EciI GGCGGA 2 cut(s) 79, 1136
EclXI CGGCCG 1 cut(s) 420
Eco130I CCWWGG 1 cut(s) 1487
Eco24I GRGCYC 2 cut(s) 377, 504
Eco47I GGWCC 2 cut(s) 328, 338
Eco52I CGGCCG 1 cut(s) 420
Eco57I CTGAAG 2 cut(s) 1104, 1241
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 3 cut(s) 260, 982, 1300
EcoT14I CCWWGG 1 cut(s) 1487
EcoT22I ATGCAT 1 cut(s) 1121
EcoT38I GRGCYC 2 cut(s) 377, 504
ErhI CCWWGG 1 cut(s) 1487
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 6 cut(s) 436, 568, 1119, 1123, 1339, 1429
FalI AAGNNNNNCTT 2 cut(s) 1480, 1512
FaqI GGGAC 3 cut(s) 122, 1189, 1497
FatI CATG 6 cut(s) 432, 564, 1115, 1119, 1335, 1425
FauI CCCGC 2 cut(s) 194, 1542
FbaI TGATCA 1 cut(s) 516
FblI GTMKAC 2 cut(s) 48, 1459
Fnu4HI GCNGC 8 cut(s) 311, 393, 648, 830, 833, 999, 1494, 1531
FokI GGATG 5 cut(s) 106, 962, 1098, 1318, 1420
FriOI GRGCYC 2 cut(s) 377, 504
Fsp4HI GCNGC 8 cut(s) 311, 393, 648, 830, 833, 999, 1494, 1531
FspBI CTAG 3 cut(s) 1002, 1466, 1549
GlaI GCGC 1 cut(s) 1306
GluI GCNGC 8 cut(s) 311, 393, 648, 830, 833, 999, 1494, 1531
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 1 cut(s) 1405
HaeII RGCGCY 1 cut(s) 1308
HaeIII GGCC 5 cut(s) 181, 422, 431, 884, 1486
HgaI GACGC 2 cut(s) 71, 266
HhaI GCGC 1 cut(s) 1307
Hin1I GRCGYC 2 cut(s) 63, 258
Hin1II CATG 6 cut(s) 436, 568, 1119, 1123, 1339, 1429
Hin6I GCGC 1 cut(s) 1305
HinP1I GCGC 1 cut(s) 1305
HincII GTYRAC 2 cut(s) 49, 577
HindII GTYRAC 2 cut(s) 49, 577
HphI GGTGA 5 cut(s) 425, 455, 686, 694, 1256
Hpy166II GTNNAC 3 cut(s) 49, 577, 1460
Hpy188III TCNNGA 4 cut(s) 326, 947, 1316, 1426
Hpy8I GTNNAC 3 cut(s) 49, 577, 1460
Hpy99I CGWCG 9 cut(s) 50, 53, 65, 74, 98, 209, 260, 401, 787
HpyAV CCTTC 8 cut(s) 610, 679, 700, 835, 934, 1079, 1160, 1235
HpyCH4III ACNGT 2 cut(s) 382, 1071
HpyCH4IV ACGT 5 cut(s) 45, 51, 116, 1160, 1285
HpyCH4V TGCA 5 cut(s) 436, 835, 908, 1119, 1289
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 647, 653, 1116
HpySE526I ACGT 5 cut(s) 45, 51, 116, 1160, 1285
Hsp92I GRCGYC 2 cut(s) 63, 258
Hsp92II CATG 6 cut(s) 436, 568, 1119, 1123, 1339, 1429
HspAI GCGC 1 cut(s) 1305
Ksp22I TGATCA 1 cut(s) 516
Kzo9I GATC 5 cut(s) 37, 106, 159, 516, 1050
LmnI GCTCC 4 cut(s) 355, 507, 653, 1180
Lsp1109I GCAGC 4 cut(s) 634, 819, 1480, 1542
LweI GCATC 1 cut(s) 1425
MaeI CTAG 3 cut(s) 1002, 1466, 1549
MaeII ACGT 5 cut(s) 45, 51, 116, 1160, 1285
MaeIII GTNAC 6 cut(s) 382, 413, 443, 529, 986, 1270
MalI GATC 5 cut(s) 39, 108, 161, 518, 1052
MbiI CCGCTC 1 cut(s) 656
MboI GATC 5 cut(s) 37, 106, 159, 516, 1050
MboII GAAGA 2 cut(s) 49, 441
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 1 cut(s) 1050
MhlI GDGCHC 2 cut(s) 377, 504
MluCI AATT 8 cut(s) 268, 301, 495, 507, 556, 588, 631, 992
MlyI GAGTC 7 cut(s) 203, 326, 410, 419, 440, 689, 1211
MmeI TCCRAC 2 cut(s) 316, 377
Mph1103I ATGCAT 1 cut(s) 1121
MseI TTAA 5 cut(s) 456, 510, 1008, 1131, 1365
MspA1I CMGCKG 1 cut(s) 832
MspR9I CCNGG 4 cut(s) 262, 332, 984, 1302
MunI CAATTG 1 cut(s) 495
MvaI CCWGG 3 cut(s) 262, 984, 1302
MwoI GCNNNNNNNGC 4 cut(s) 428, 647, 653, 1116
NciI CCSGG 1 cut(s) 332
NdeII GATC 5 cut(s) 37, 106, 159, 516, 1050
NlaIII CATG 6 cut(s) 436, 568, 1119, 1123, 1339, 1429
NlaIV GGNNCC 3 cut(s) 330, 345, 503
NmeAIII GCCGAG 3 cut(s) 215, 398, 407
NmuCI GTSAC 5 cut(s) 413, 443, 529, 986, 1270
NsiI ATGCAT 1 cut(s) 1121
NspI RCATGY 2 cut(s) 568, 1123
PagI TCATGA 1 cut(s) 1425
PcsI WCGNNNNNNNCGW 6 cut(s) 42, 57, 66, 69, 102, 138
PfeI GAWTC 8 cut(s) 143, 322, 720, 1025, 1042, 1216, 1328, 1346
PflFI GACNNNGTC 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 8 cut(s) 312, 394, 649, 831, 834, 1000, 1495, 1532
PleI GAGTC 7 cut(s) 203, 325, 410, 419, 440, 689, 1210
PpsI GAGTC 7 cut(s) 203, 325, 410, 419, 440, 689, 1210
PpuMI RGGWCCY 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 3 cut(s) 260, 982, 1300
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 3 cut(s) 260, 982, 1300
PspN4I GGNNCC 3 cut(s) 330, 345, 503
PspPI GGNCC 3 cut(s) 180, 328, 338
PspPPI RGGWCCY 1 cut(s) 328
PsrI GAACNNNNNNTAC 2 cut(s) 845, 877
PstI CTGCAG 1 cut(s) 837
PstNI CAGNNNCTG 1 cut(s) 1075
PsuI RGATCY 1 cut(s) 1050
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 4 cut(s) 815, 1034, 1068, 1542
RsaNI GTAC 4 cut(s) 814, 1033, 1067, 1541
Rsr2I CGGWCCG 1 cut(s) 338
RsrII CGGWCCG 1 cut(s) 338
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 5 cut(s) 456, 510, 1008, 1131, 1365
SatI GCNGC 8 cut(s) 311, 393, 648, 830, 833, 999, 1494, 1531
Sau3AI GATC 5 cut(s) 37, 106, 159, 516, 1050
Sau96I GGNCC 3 cut(s) 180, 328, 338
SchI GAGTC 7 cut(s) 203, 326, 410, 419, 440, 689, 1211
ScrFI CCNGG 4 cut(s) 262, 332, 984, 1302
SduI GDGCHC 2 cut(s) 377, 504
SfaNI GCATC 1 cut(s) 1425
SfcI CTRYAG 1 cut(s) 833
SfiI GGCCNNNNNGGCC 1 cut(s) 428
SgrDI CGTCGACG 1 cut(s) 47
SinI GGWCC 2 cut(s) 328, 338
Sse9I AATT 8 cut(s) 268, 301, 495, 507, 556, 588, 631, 992
SspMI CTAG 3 cut(s) 1002, 1466, 1549
StyD4I CCNGG 4 cut(s) 260, 330, 982, 1300
StyI CCWWGG 1 cut(s) 1487
TaaI ACNGT 2 cut(s) 382, 1071
TaiI ACGT 5 cut(s) 48, 54, 119, 1163, 1288
TaqI TCGA 8 cut(s) 48, 60, 69, 132, 320, 377, 825, 1278
TaqII GACCGA 2 cut(s) 371, 420
TasI AATT 8 cut(s) 268, 301, 495, 507, 556, 588, 631, 992
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 4 cut(s) 313, 395, 832, 1001
TfiI GAWTC 8 cut(s) 143, 322, 720, 1025, 1042, 1216, 1328, 1346
Tru1I TTAA 5 cut(s) 456, 510, 1008, 1131, 1365
Tru9I TTAA 5 cut(s) 456, 510, 1008, 1131, 1365
TseFI GTSAC 5 cut(s) 413, 443, 529, 986, 1270
TseI GCWGC 4 cut(s) 647, 832, 1493, 1530
Tsp45I GTSAC 5 cut(s) 413, 443, 529, 986, 1270
TspDTI ATGAA 5 cut(s) 460, 854, 1153, 1320, 1414
TspGWI ACGGA 1 cut(s) 161
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 328, 338
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 2 cut(s) 301, 631
XceI RCATGY 2 cut(s) 568, 1123
XmiI GTMKAC 2 cut(s) 48, 1459
XspI CTAG 3 cut(s) 1002, 1466, 1549
Zsp2I ATGCAT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.