pycom10g13380

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008764
Physical Location & Seq
Reverse (-)
16813799 .. 16814593
795 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGGCTAATATGGTTGAGGCATTGAGCCAGTGCGGTAAAAGGGTTGAAGTTGCCACCAGAAGAGCAGAGGCCATGGTGGATAATGTCTGGAACCACTTGAGAATGTGTCCAAGTTTCACAGAATCAGCGAAGGCAAGGCTAAATACCGGCACAAAGGTGCATACTACTGGAGGACAACAAAAAGTATTCCAGCAGACATTTGAAAGAATTACAGGAGAGAAGCTCTTACATTCATATGCTTGCTATCTATCAACTTCTCATGGATCTGTGATTGGGATGCTGTGCATCTCCAATGTACGAACAGCCTTCTGCAGTGATTTCTCACACTATTTGTCCCCAGGACATCCAACTTCCATGCATTACAAGAACATAAATCCTTCAACAAACATATGGAACCCTTCAGAAAGGTATATACACATTGTCACAAGAGAGGGTCATGAATTCTGGTTCATGGGGTTCATATCCTATGACAAGGCCCTCGGTAATCTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

165

Amino Acids

18.55

Weight (kDa)

8.87

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 62 - 159 1e-08 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 33
AclWI GGATC 1 cut(s) 271
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 1 cut(s) 297
AgsI TTSAA 3 cut(s) 47, 203, 381
AjnI CCWGG 1 cut(s) 337
AleI CACNNNNGTG 1 cut(s) 155
AluBI AGCT 2 cut(s) 223, 492
AluI AGCT 2 cut(s) 223, 492
AlwI GGATC 1 cut(s) 271
AoxI GGCC 2 cut(s) 69, 474
ApoI RAATTY 1 cut(s) 440
AspS9I GGNCC 1 cut(s) 475
BciT130I CCWGG 1 cut(s) 339
BfmI CTRYAG 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 2 cut(s) 92, 395
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 267, 294
BplI GAGNNNNNCTC 2 cut(s) 207, 239
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 72, 337, 478
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 28, 172
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 72, 337, 478
BseGI GGATG 2 cut(s) 282, 343
BseNI ACTGG 2 cut(s) 28, 172
BshFI GGCC 2 cut(s) 71, 476
BsiSI CCGG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 2 cut(s) 71, 476
Bsp143I GATC 1 cut(s) 263
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 71, 476
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 92, 395
BspMAI CTGCAG 1 cut(s) 314
BspPI GGATC 1 cut(s) 271
BspQI GCTCTTC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 2 cut(s) 28, 172
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 72, 337, 478
BssMI GATC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 339
Bst6I CTCTTC 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 488
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 282, 343
BstKTI GATC 1 cut(s) 266
BstMBI GATC 1 cut(s) 263
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 2 cut(s) 71, 476
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 282, 343
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 35, 319
Cac8I GCNNGC 1 cut(s) 241
CciI TCATGA 1 cut(s) 436
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 475
Csp6I GTAC 1 cut(s) 296
CviAII CATG 5 cut(s) 73, 260, 355, 437, 451
CviJI RGCY 8 cut(s) 5, 27, 71, 139, 223, 305, 476, 492
CviKI_1 RGCY 8 cut(s) 5, 27, 71, 139, 223, 305, 476, 492
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 1 cut(s) 488
DpnI GATC 1 cut(s) 265
DpnII GATC 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 475
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 5 cut(s) 76, 263, 358, 440, 454
FaqI GGGAC 1 cut(s) 319
FatI CATG 5 cut(s) 72, 259, 354, 436, 450
FauNDI CATATG 2 cut(s) 235, 389
FokI GGATG 2 cut(s) 289, 330
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 2 cut(s) 71, 476
HapII CCGG 1 cut(s) 147
Hin1II CATG 5 cut(s) 76, 263, 358, 440, 454
HinfI GANTC 1 cut(s) 122
HpaII CCGG 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 2 cut(s) 88, 437
HpyAV CCTTC 4 cut(s) 124, 316, 387, 408
HpyCH4V TGCA 4 cut(s) 160, 285, 312, 358
HpyF3I CTNAG 1 cut(s) 488
Hsp92II CATG 5 cut(s) 76, 263, 358, 440, 454
Kzo9I GATC 1 cut(s) 263
LguI GCTCTTC 1 cut(s) 55
LweI GCATC 2 cut(s) 267, 294
MaeIII GTNAC 1 cut(s) 421
MalI GATC 1 cut(s) 265
MboI GATC 1 cut(s) 263
MboII GAAGA 1 cut(s) 72
MflI RGATCY 1 cut(s) 263
MluCI AATT 2 cut(s) 207, 440
MmeI TCCRAC 1 cut(s) 371
MnlI CCTC 5 cut(s) 10, 61, 164, 424, 488
Mph1103I ATGCAT 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 155, 234
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 235, 389
NdeII GATC 1 cut(s) 263
NlaIII CATG 5 cut(s) 76, 263, 358, 440, 454
NlaIV GGNNCC 2 cut(s) 92, 395
NmuCI GTSAC 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 155
PagI TCATGA 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 92, 395
PspPI GGNCC 1 cut(s) 475
PstI CTGCAG 1 cut(s) 314
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
RseI CAYNNNNRTG 2 cut(s) 155, 234
SapI GCTCTTC 1 cut(s) 55
Sau3AI GATC 1 cut(s) 263
Sau96I GGNCC 1 cut(s) 475
ScrFI CCNGG 1 cut(s) 339
SetI ASST 4 cut(s) 159, 225, 410, 494
SfaNI GCATC 2 cut(s) 267, 294
SfcI CTRYAG 1 cut(s) 310
SmiMI CAYNNNNRTG 2 cut(s) 155, 234
SmlI CTYRAG 1 cut(s) 97
SmoI CTYRAG 1 cut(s) 97
Sse9I AATT 2 cut(s) 207, 440
SsiI CCGC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 72
TasI AATT 2 cut(s) 207, 440
TfiI GAWTC 1 cut(s) 122
TscAI CASTG 2 cut(s) 35, 319
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 4 cut(s) 222, 439, 448, 453
TspRI CASTG 2 cut(s) 35, 319
XapI RAATTY 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.