pycom10g14260

Thiamine-repressible mitochondrial transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005884
Physical Location & Seq
Reverse (-)
17642734 .. 17644264
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGCAATTCTCTGTTTGTGATCGATCCTCATACATTCCCTTGGTTGAAATCGGGCGGTATTTGGAGGATAATTTTGAGGGGTTTTGGATTTGGAGAAGTAGGAAGAGTAGCCCTTTGCAAAGATTAGGAGATTCGGAGCAAATCACTCTCCTTGAGGAGACTGACGGAGTTGTGAAAGCAGATGCGGAAGAGGGGGAAGTTAATCTTGGTGCAGAACCGAAAGTTGTATTGTACGAGCTTGTTGGAACTTCAGCAACTCAAGACAATGGCACTGAAACGGTAGATAAGCAAGTGGATGAAAAAGGGCGTTGGACACGAACTCGAGTGGCTAAAGTTAGCCTACTGATTTCCCCGTTTTGGTTTTTCGCACAGCTGACTTTCAATTTGTCATTGAAGTATACTACCGTTACGTCGAATACAATCTTAAGCAGTTCATCCAGCCTTTTCACCTTTTTGGTCGCCCTAGTATTCTTAGGGGAGAAGTTTACTTTGGTTAAGCTTATTAGCGTTCTTCTTTGCATGGGAGGAACGATAATTGTCAGCCTTGGCGACTCACGAACTGCTCTAAGTGCAATTGCTTCAAATCCTCTCCTCGGAGACATACTTGCTCTTGTCTCGGCGGCCTTCTATTCTGTGTATATTACCCTCATTCGCAAGAAATTACCTGATGAGGATGATGAAAAAAGCGGCCGTGCCAGTATGGCTCAGTTTCTAGGATTTCTAGGGCTTTCCAACCTTCTTATATTTCTTCCGGTTGCCCTTATACTTCATTTCTCCAAGCTGGAGCCGTTTTATATGCTTACCTGGGAGCAGGTCGGTCTCATCGTTGGTAAAGGTTTGTTGGATAATGTTCTGAGCGACTACTTATGGGCCAAGGCCGTTCTTCTAACCACCACGACGGTAGCAACGGCCGGTCTGACCATCCAGGTTCCGTTGGCGGCAATTGTTGACTCAATGACTGGCCATGTTCCTCATTTTGCTGATTACCTTGGAGCCGTGGCGGTCATGATCGGATTCGCTGGCATAAACATTCCCGCTGATGCTTTTAACAGACCAAGAGAAGCTATACTCGCGAGAGAGAATGAAAATATCAGTGCAACCGGTGAAGCTCGCAATTCATCAGGCAGCCCGATTTCAGCTGCCCCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

384

Amino Acids

41.62

Weight (kDa)

4.96

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 98 - 219 7.6e-08 Purine nucleobase transmembrane transport
EamA PF00892 112 - 181 8.7e-10 EamA-like transporter family
TPT PF03151 117 - 263 4.6e-06 Triose-phosphate Transporter family
SLC35F PF06027 131 - 248 5.5e-09 Solute carrier family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 802
AccI GTMKAC 1 cut(s) 398
AccII CGCG 1 cut(s) 1073
AciI CCGC 7 cut(s) 55, 185, 620, 687, 938, 1001, 1035
AclWI GGATC 1 cut(s) 18
AcoI YGGCCR 3 cut(s) 688, 909, 961
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 357, 593
AflII CTTAAG 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 1100
AgsI TTSAA 4 cut(s) 47, 382, 394, 582
AjnI CCWGG 2 cut(s) 803, 924
AjuI GAANNNNNNNTTGG 4 cut(s) 189, 221, 473, 505
AluBI AGCT 7 cut(s) 238, 373, 499, 781, 1064, 1109, 1139
AluI AGCT 7 cut(s) 238, 373, 499, 781, 1064, 1109, 1139
Alw26I GTCTC 4 cut(s) 152, 591, 619, 824
AlwI GGATC 1 cut(s) 18
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 6 cut(s) 621, 688, 870, 876, 909, 961
ApeKI GCWGC 2 cut(s) 1125, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AsiGI ACCGGT 1 cut(s) 1100
AspS9I GGNCC 1 cut(s) 870
AsuHPI GGTGA 2 cut(s) 439, 1115
AvaI CYCGRG 1 cut(s) 321
BalI TGGCCA 1 cut(s) 963
BbvI GCAGC 2 cut(s) 1126, 1137
BccI CCATC 1 cut(s) 929
BceAI ACGGC 5 cut(s) 675, 772, 863, 924, 980
BciT130I CCWGG 2 cut(s) 805, 926
BcoDI GTCTC 4 cut(s) 152, 591, 619, 824
BfaI CTAG 3 cut(s) 464, 713, 722
BfrI CTTAAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 802
BglI GCCNNNNNGGC 1 cut(s) 701
BisI GCNGC 5 cut(s) 621, 688, 939, 1126, 1140
BlsI GCNGC 5 cut(s) 622, 689, 940, 1127, 1141
Bme1390I CCNGG 2 cut(s) 805, 926
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 870
BmiI GGNNCC 3 cut(s) 786, 930, 994
BmrFI CCNGG 2 cut(s) 805, 926
BmsI GCATC 2 cut(s) 172, 1030
BpmI CTGGAG 1 cut(s) 803
BpuEI CTTGAG 2 cut(s) 173, 243
Bsa29I ATCGAT 1 cut(s) 22
BsaI GGTCTC 1 cut(s) 824
BsaJI CCNNGG 7 cut(s) 39, 544, 592, 804, 873, 988, 996
BsaWI WCCGGW 2 cut(s) 751, 1100
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 593
Bse118I RCCGGY 2 cut(s) 911, 1100
Bse1I ACTGG 2 cut(s) 696, 964
BseBI CCWGG 2 cut(s) 805, 926
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 7 cut(s) 39, 544, 592, 804, 873, 988, 996
BseGI GGATG 4 cut(s) 301, 434, 679, 921
BseLI CCNNNNNNNGG 2 cut(s) 357, 593
BseMII CTCAG 2 cut(s) 719, 845
BseNI ACTGG 2 cut(s) 696, 964
BseRI GAGGAG 2 cut(s) 170, 581
BseX3I CGGCCG 2 cut(s) 688, 909
BseXI GCAGC 2 cut(s) 1126, 1137
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 1073
Bsh1285I CGRYCG 2 cut(s) 691, 912
BshFI GGCC 6 cut(s) 623, 690, 872, 878, 911, 963
BshTI ACCGGT 1 cut(s) 1100
BshVI ATCGAT 1 cut(s) 22
BsiEI CGRYCG 2 cut(s) 691, 912
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 3 cut(s) 752, 912, 1101
BslI CCNNNNNNNGG 2 cut(s) 357, 593
BsmAI GTCTC 4 cut(s) 152, 591, 619, 824
BsnI GGCC 6 cut(s) 623, 690, 872, 878, 911, 963
Bso31I GGTCTC 1 cut(s) 824
BsoBI CYCGRG 1 cut(s) 321
Bsp143I GATC 3 cut(s) 19, 23, 1008
Bsp68I TCGCGA 1 cut(s) 1073
BspACI CCGC 7 cut(s) 55, 185, 620, 687, 938, 1001, 1035
BspANI GGCC 6 cut(s) 623, 690, 872, 878, 911, 963
BspCNI CTCAG 2 cut(s) 718, 846
BspDI ATCGAT 1 cut(s) 22
BspFNI CGCG 1 cut(s) 1073
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 3 cut(s) 786, 930, 994
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 1 cut(s) 18
BspTI CTTAAG 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 824
BsrFI RCCGGY 2 cut(s) 911, 1100
BsrI ACTGG 2 cut(s) 696, 964
BssAI RCCGGY 2 cut(s) 911, 1100
BssECI CCNNGG 7 cut(s) 39, 544, 592, 804, 873, 988, 996
BssMI GATC 3 cut(s) 19, 23, 1008
BssNAI GTATAC 1 cut(s) 399
BssT1I CCWWGG 4 cut(s) 39, 544, 873, 988
Bst1107I GTATAC 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 805, 926
Bst4CI ACNGT 3 cut(s) 280, 406, 901
Bst6I CTCTTC 2 cut(s) 98, 183
BstAFI CTTAAG 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 1021, 1111
BstDEI CTNAG 4 cut(s) 472, 566, 705, 854
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 4 cut(s) 301, 434, 679, 921
BstFNI CGCG 1 cut(s) 1073
BstKTI GATC 3 cut(s) 22, 26, 1011
BstMAI GTCTC 4 cut(s) 152, 591, 619, 824
BstMBI GATC 3 cut(s) 19, 23, 1008
BstMCI CGRYCG 2 cut(s) 691, 912
BstMWI GCNNNNNNNGC 3 cut(s) 569, 701, 1070
BstNI CCWGG 2 cut(s) 805, 926
BstSCI CCNGG 2 cut(s) 803, 924
BstUI CGCG 1 cut(s) 1073
BstV1I GCAGC 2 cut(s) 1126, 1137
BstZ17I GTATAC 1 cut(s) 399
BstZI CGGCCG 2 cut(s) 688, 909
Bsu15I ATCGAT 1 cut(s) 22
BsuRI GGCC 6 cut(s) 623, 690, 872, 878, 911, 963
BsuTUI ATCGAT 1 cut(s) 22
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 4 cut(s) 301, 434, 679, 921
BtsIMutI CAGTG 2 cut(s) 270, 1099
BtuMI TCGCGA 1 cut(s) 1073
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 1021, 1111
CciI TCATGA 1 cut(s) 1005
Cfr10I RCCGGY 2 cut(s) 911, 1100
Cfr13I GGNCC 1 cut(s) 870
ClaI ATCGAT 1 cut(s) 22
Csp6I GTAC 1 cut(s) 232
CspAI ACCGGT 1 cut(s) 1100
CviAII CATG 3 cut(s) 520, 965, 1006
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 4 cut(s) 472, 566, 705, 854
DpnI GATC 3 cut(s) 21, 25, 1010
DpnII GATC 3 cut(s) 19, 23, 1008
EaeI YGGCCR 3 cut(s) 688, 909, 961
EagI CGGCCG 2 cut(s) 688, 909
Eam1104I CTCTTC 2 cut(s) 98, 183
EarI CTCTTC 2 cut(s) 98, 183
EclXI CGGCCG 2 cut(s) 688, 909
Eco130I CCWWGG 4 cut(s) 39, 544, 873, 988
Eco31I GGTCTC 1 cut(s) 824
Eco52I CGGCCG 2 cut(s) 688, 909
Eco57I CTGAAG 1 cut(s) 234
Eco88I CYCGRG 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 803, 924
EcoT14I CCWWGG 4 cut(s) 39, 544, 873, 988
ErhI CCWWGG 4 cut(s) 39, 544, 873, 988
FaeI CATG 3 cut(s) 523, 968, 1009
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 3 cut(s) 519, 964, 1005
FauI CCCGC 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 5 cut(s) 621, 688, 939, 1126, 1140
FokI GGATG 4 cut(s) 308, 421, 686, 908
Fsp4HI GCNGC 5 cut(s) 621, 688, 939, 1126, 1140
FspBI CTAG 3 cut(s) 464, 713, 722
GluI GCNGC 5 cut(s) 621, 688, 939, 1126, 1140
GsuI CTGGAG 1 cut(s) 803
HaeIII GGCC 6 cut(s) 623, 690, 872, 878, 911, 963
HapII CCGG 3 cut(s) 752, 912, 1101
Hin1II CATG 3 cut(s) 523, 968, 1009
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 4 cut(s) 131, 551, 950, 1014
HpaII CCGG 3 cut(s) 752, 912, 1101
HphI GGTGA 2 cut(s) 439, 1115
Hpy166II GTNNAC 3 cut(s) 399, 486, 949
Hpy188I TCNGA 5 cut(s) 136, 596, 855, 918, 1013
Hpy188III TCNNGA 4 cut(s) 260, 555, 1006, 1072
Hpy8I GTNNAC 3 cut(s) 399, 486, 949
Hpy99I CGWCG 2 cut(s) 415, 901
HpyAV CCTTC 2 cut(s) 634, 746
HpyCH4III ACNGT 3 cut(s) 280, 406, 901
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 6 cut(s) 4, 118, 212, 519, 572, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 701, 1070
HpyF3I CTNAG 4 cut(s) 472, 566, 705, 854
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 3 cut(s) 523, 968, 1009
Kzo9I GATC 3 cut(s) 19, 23, 1008
LmnI GCTCC 4 cut(s) 136, 784, 808, 992
Lsp1109I GCAGC 2 cut(s) 1126, 1137
LweI GCATC 2 cut(s) 172, 1030
MaeI CTAG 3 cut(s) 464, 713, 722
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 406
MalI GATC 3 cut(s) 21, 25, 1010
MboI GATC 3 cut(s) 19, 23, 1008
MboII GAAGA 5 cut(s) 115, 200, 503, 740, 875
MfeI CAATTG 2 cut(s) 573, 942
MlsI TGGCCA 1 cut(s) 963
MluCI AATT 8 cut(s) 5, 70, 382, 534, 573, 659, 942, 1114
MluNI TGGCCA 1 cut(s) 963
MlyI GAGTC 2 cut(s) 545, 944
MmeI TCCRAC 4 cut(s) 223, 290, 756, 822
Mox20I TGGCCA 1 cut(s) 963
MscI TGGCCA 1 cut(s) 963
MseI TTAA 4 cut(s) 201, 425, 495, 1047
Msp20I TGGCCA 1 cut(s) 963
MspA1I CMGCKG 3 cut(s) 373, 1037, 1139
MspCI CTTAAG 1 cut(s) 424
MspI CCGG 3 cut(s) 752, 912, 1101
MspR9I CCNGG 2 cut(s) 805, 926
MunI CAATTG 2 cut(s) 573, 942
MvaI CCWGG 2 cut(s) 805, 926
MvnI CGCG 1 cut(s) 1073
MwoI GCNNNNNNNGC 3 cut(s) 569, 701, 1070
NdeII GATC 3 cut(s) 19, 23, 1008
NlaIII CATG 3 cut(s) 523, 968, 1009
NlaIV GGNNCC 3 cut(s) 786, 930, 994
NmeAIII GCCGAG 1 cut(s) 596
NruI TCGCGA 1 cut(s) 1073
PaeR7I CTCGAG 1 cut(s) 321
PagI TCATGA 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PfeI GAWTC 2 cut(s) 131, 1014
PinAI ACCGGT 1 cut(s) 1100
PkrI GCNGC 5 cut(s) 622, 689, 940, 1127, 1141
PleI GAGTC 2 cut(s) 545, 944
PpsI GAGTC 2 cut(s) 545, 944
Psp6I CCWGG 2 cut(s) 803, 924
PspGI CCWGG 2 cut(s) 803, 924
PspN4I GGNNCC 3 cut(s) 786, 930, 994
PspPI GGNCC 1 cut(s) 870
PspXI VCTCGAGB 1 cut(s) 321
PvuII CAGCTG 2 cut(s) 373, 1139
RruI TCGCGA 1 cut(s) 1073
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SaqAI TTAA 4 cut(s) 201, 425, 495, 1047
SatI GCNGC 5 cut(s) 621, 688, 939, 1126, 1140
Sau3AI GATC 3 cut(s) 19, 23, 1008
Sau96I GGNCC 1 cut(s) 870
SchI GAGTC 2 cut(s) 545, 944
ScrFI CCNGG 2 cut(s) 805, 926
SfaNI GCATC 2 cut(s) 172, 1030
Sfr274I CTCGAG 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 4 cut(s) 152, 258, 321, 424
SmoI CTYRAG 4 cut(s) 152, 258, 321, 424
Sse9I AATT 8 cut(s) 5, 70, 382, 534, 573, 659, 942, 1114
SsiI CCGC 7 cut(s) 55, 185, 620, 687, 938, 1001, 1035
SspMI CTAG 3 cut(s) 464, 713, 722
StyD4I CCNGG 2 cut(s) 803, 924
StyI CCWWGG 4 cut(s) 39, 544, 873, 988
TaaI ACNGT 3 cut(s) 280, 406, 901
TaiI ACGT 1 cut(s) 413
TaqI TCGA 3 cut(s) 22, 322, 413
TaqII GACCGA 1 cut(s) 806
TasI AATT 8 cut(s) 5, 70, 382, 534, 573, 659, 942, 1114
TauI GCSGC 3 cut(s) 623, 690, 941
TfiI GAWTC 2 cut(s) 131, 1014
Tru1I TTAA 4 cut(s) 201, 425, 495, 1047
Tru9I TTAA 4 cut(s) 201, 425, 495, 1047
TscAI CASTG 2 cut(s) 277, 1099
TseI GCWGC 2 cut(s) 1125, 1139
TspDTI ATGAA 7 cut(s) 312, 423, 693, 758, 1098, 1107, 1137
TspGWI ACGGA 2 cut(s) 180, 921
TspRI CASTG 2 cut(s) 277, 1099
Vha464I CTTAAG 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 3 cut(s) 464, 713, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.