pycom10g14700

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001638
Physical Location & Seq
Reverse (-)
18083681 .. 18090402
6722 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1599 bp
ATGAATGGGTGCGTCCCCGATTTCGAAATGGACGACGATTACTCACTTCCCACTTCCTCTGCTCTCAACCGACCCAGAAAATCAACCATGCCGGAAGACGATGTCATGGAGCTGCTATGGCAGAACGGCCAAGTCGTCATGCAGAGCCAGAACCAGAGGTCGGCAGTCAATAGTAAAAAATCGCACCAGTCTAAGCACGACGTCGTTTTGCCCGACGACGGTGGCGGCATCACCAGGCCGGCCTCCCTACCCCAGCCGCCGGCCCAAAACCCGCACCTGTTTATGCAAGAAGATGAAATGGCCTCGTGGCTTCAGTACCCGCTCGTCGACGACCCCTTCTCCGCGGACCTCCTCTATCCTGATTCCACCACTTCCGAGCTCCGGCAGCCGCAGGTTTCGGCGCCCGCGCCCTCTTCGAGGTTGCCGATCCATATGCCGAGGAGGACCGAGGTCCAGAACTTTCTGCACTTCGGCAGGCCCAACAACAACAACGCCAGCAGCAACAGGCCTATGATTCCGGAAATGGCGCCATCGAGCTCGAAGAAGAGCGTGGTTAGGGAGACGATGACGGTGGTGGACTCCAGCGACACTCCGCTGGTGGGTCCAAGCTCTAGGGCTTTGGATTCTAGGCCCGACGGCGCTGGCGGCGGCTTGGCTAATGGAGCGACGCCGTTTCCAACTGCTGGGACGTCGTTTCCGGCAAAAGAAGTGACCACGTGTGAGATGTCGCTGACGTCGTCGCCAGGAGGCTCCAGCGAGAGTGCCAGCGCGAGCGCCGAGCCGATGTCGGCTCAGAAGCCGCCGCCGACTGCCGATAACCGGAAGCGGAAAGGGAGAGAAGTCGCCGCCGCCGCCGACGATGCCGAGATTCAATGCGAGGATGTTGAGTTTGAATCTGCCAATGGAAAGAAACAAGTCCGAGGATCGACATCCTCTACAAAGAGATCGCGTGCTGCAGAGGTCCACAATCTCTCTGAGAGGAGACGTCGAGATAGGATAAATGAAAAGATGAAGGCTTTACAAGAACTAATACCTCGATGCAATAAGTCGGATAAAGCATCAATGCTGGATGAAGCAATTGAGTACTTGAAATCACTCCAGTTACAAGTACAGATGGTGTCCATGGGATGCGGCATGGTCCCTATGATGTTTCCTGGAGTTCAGCAGATGATGTCCATGCCGATGGGGATGGGAATTGGAATGGGCATGGGAATGGGAATGGGAATGGAAATGGCCAGCATCAATCGCCCTATGATGCCTTTTCCAAATGTAATAGCCGGTTCACCCATGCCAACAGCGGCCGCACATATGGGACCTAGGTTTCCTATACCACCATTTCACATGCAGCCTATTCCTGCAAGTGATCCTGCCAGAGTTCCAGCAGTGAACCAGACAGATCATATGATAAACTCGCTCGGGGCACAAAATCCAAACCAATCACGCATGCCCAATTTTGCAGATCCTTATCAGCAGTTTTTCGGTCCCCAACAGATGCAGTTACCATTACAGCAGCAGAACCAAGCAATGCCCCAGCCAACTACTGGCAAGCCGAGTTCCAGTAGGGGCCCTGAAACTCACGAAAACCATAAGTCAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006725 GO:0006778 GO:0006779 GO:0006783 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009889 GO:0009959 GO:0009987 GO:0010017 GO:0010029 GO:0010033 GO:0010099 GO:0010100 GO:0010114 GO:0010161 GO:0010187 GO:0010313 GO:0010468 GO:0010476 GO:0010556 GO:0015994 GO:0015995 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0033013 GO:0033014 GO:0033993 GO:0034641 GO:0042168 GO:0042221 GO:0042440 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0046148 GO:0046483 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051186 GO:0051188 GO:0051239 GO:0051241 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071478 GO:0071482 GO:0071489 GO:0071491 GO:0071495 GO:0071704 GO:0080090 GO:0097159 GO:0104004 GO:0140110 GO:1900140 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901701 GO:1903506 GO:2000026 GO:2000030 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
HLH

Protein Analysis

533

Amino Acids

57.81

Weight (kDa)

6.16

Isoelectric Point (pI)

61.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 320 - 367 1.5e-13 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 326 - 369 7.6e-08 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 4 cut(s) 204, 692, 737, 988
Acc36I ACCTGC 1 cut(s) 382
AccB1I GGYRCC 2 cut(s) 400, 526
AccB7I CCANNNNNTGG 2 cut(s) 683, 1541
AccBSI CCGCTC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 327
AccII CGCG 4 cut(s) 344, 407, 770, 949
AccIII TCCGGA 1 cut(s) 517
AclWI GGATC 4 cut(s) 421, 931, 1358, 1454
AcoI YGGCCR 3 cut(s) 127, 1233, 1299
AcuI CTGAAG 1 cut(s) 296
AcvI CACGTG 1 cut(s) 717
AcyI GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
AfaI GTAC 3 cut(s) 317, 1085, 1110
AfiI CCNNNNNNNGG 9 cut(s) 160, 218, 259, 381, 417, 599, 683, 819, 1541
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 3 cut(s) 872, 893, 1090
AjnI CCWGG 3 cut(s) 233, 742, 1153
AluBI AGCT 4 cut(s) 112, 379, 537, 609
AluI AGCT 4 cut(s) 112, 379, 537, 609
Alw21I GWGCWC 2 cut(s) 381, 539
Alw26I GTCTC 2 cut(s) 554, 976
AlwI GGATC 4 cut(s) 421, 931, 1358, 1454
Ama87I CYCGRG 1 cut(s) 1415
Aor13HI TCCGGA 1 cut(s) 517
ApaI GGGCCC 1 cut(s) 1568
ApeKI GCWGC 6 cut(s) 112, 385, 498, 953, 1345, 1510
AspA2I CCTAGG 1 cut(s) 1316
AspLEI GCGC 6 cut(s) 403, 409, 529, 641, 770, 776
AsuHPI GGTGA 2 cut(s) 223, 1275
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 1415
AvaII GGWCC 8 cut(s) 346, 444, 451, 602, 961, 1138, 1313, 1481
AvrII CCTAGG 1 cut(s) 1316
BaeGI GKGCMC 2 cut(s) 1423, 1568
BalI TGGCCA 1 cut(s) 1235
BanI GGYRCC 2 cut(s) 400, 526
BanII GRGCYC 3 cut(s) 381, 539, 1568
BauI CACGAG 1 cut(s) 304
BbrPI CACGTG 1 cut(s) 717
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 381, 539
BbvI GCAGC 6 cut(s) 99, 397, 510, 940, 1357, 1522
BccI CCATC 4 cut(s) 538, 1108, 1177, 1183
BceAI ACGGC 3 cut(s) 142, 652, 655
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 3 cut(s) 235, 744, 1155
BcoDI GTCTC 2 cut(s) 554, 976
BfaI CTAG 3 cut(s) 612, 627, 1317
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 4 cut(s) 404, 530, 642, 777
BfuAI ACCTGC 1 cut(s) 382
BlnI CCTAGG 1 cut(s) 1316
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 3 cut(s) 235, 744, 1155
Bme18I GGWCC 8 cut(s) 346, 444, 451, 602, 961, 1138, 1313, 1481
BmeT110I CYCGRG 1 cut(s) 1415
BmiI GGNNCC 9 cut(s) 402, 528, 603, 751, 1140, 1314, 1483, 1565, 1566
BmrFI CCNGG 3 cut(s) 235, 744, 1155
BmsI GCATC 8 cut(s) 237, 850, 1028, 1067, 1118, 1245, 1248, 1482
BoxI GACNNNNGTC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 4 cut(s) 565, 736, 1082, 1176
Bpu14I TTCGAA 1 cut(s) 24
BsaAI YACGTR 1 cut(s) 717
BsaBI GATNNNNATC 2 cut(s) 928, 1464
BsaHI GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
BsaJI CCNNGG 6 cut(s) 342, 437, 447, 919, 1122, 1316
BsaWI WCCGGW 2 cut(s) 517, 819
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 9 cut(s) 160, 218, 259, 381, 417, 599, 683, 819, 1541
Bse118I RCCGGY 3 cut(s) 238, 259, 1277
Bse1I ACTGG 4 cut(s) 187, 1099, 1546, 1557
Bse3DI GCAATG 1 cut(s) 1530
Bse8I GATNNNNATC 2 cut(s) 928, 1464
BseAI TCCGGA 1 cut(s) 517
BseBI CCWGG 3 cut(s) 235, 744, 1155
BseDI CCNNGG 6 cut(s) 342, 437, 447, 919, 1122, 1316
BseGI GGATG 5 cut(s) 886, 929, 1075, 1133, 1194
BseJI GATNNNNATC 2 cut(s) 928, 1464
BseLI CCNNNNNNNGG 9 cut(s) 160, 218, 259, 381, 417, 599, 683, 819, 1541
BseMI GCAATG 1 cut(s) 1530
BseMII CTCAG 2 cut(s) 806, 966
BseNI ACTGG 4 cut(s) 187, 1099, 1546, 1557
BseRI GAGGAG 3 cut(s) 341, 454, 994
BseSI GKGCMC 2 cut(s) 1423, 1568
BseX3I CGGCCG 1 cut(s) 1299
BseXI GCAGC 6 cut(s) 99, 397, 510, 940, 1357, 1522
BseYI CCCAGC 3 cut(s) 252, 683, 1530
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 4 cut(s) 344, 407, 770, 949
Bsh1285I CGRYCG 1 cut(s) 1302
BshNI GGYRCC 2 cut(s) 400, 526
BsiEI CGRYCG 1 cut(s) 1302
BsiHKAI GWGCWC 2 cut(s) 381, 539
BsiHKCI CYCGRG 1 cut(s) 1415
BsiSI CCGG 8 cut(s) 92, 239, 260, 382, 518, 698, 820, 1278
BslFI GGGAC 4 cut(s) 700, 1124, 1326, 1467
BslI CCNNNNNNNGG 9 cut(s) 160, 218, 259, 381, 417, 599, 683, 819, 1541
BsmAI GTCTC 2 cut(s) 554, 976
BsmBI CGTCTC 2 cut(s) 554, 976
BsmFI GGGAC 4 cut(s) 700, 1124, 1326, 1467
BsoBI CYCGRG 1 cut(s) 1415
Bsp119I TTCGAA 1 cut(s) 24
Bsp120I GGGCCC 1 cut(s) 1564
Bsp1286I GDGCHC 4 cut(s) 381, 539, 1423, 1568
Bsp13I TCCGGA 1 cut(s) 517
Bsp143I GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
Bsp19I CCATGG 1 cut(s) 1122
BspCNI CTCAG 2 cut(s) 805, 967
BspEI TCCGGA 1 cut(s) 517
BspFNI CGCG 4 cut(s) 344, 407, 770, 949
BspLI GGNNCC 9 cut(s) 402, 528, 603, 751, 1140, 1314, 1483, 1565, 1566
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 4 cut(s) 421, 931, 1358, 1454
BspQI GCTCTTC 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 2 cut(s) 400, 526
BsrBI CCGCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 1530
BsrFI RCCGGY 3 cut(s) 238, 259, 1277
BsrI ACTGG 4 cut(s) 187, 1099, 1546, 1557
BssAI RCCGGY 3 cut(s) 238, 259, 1277
BssECI CCNNGG 6 cut(s) 342, 437, 447, 919, 1122, 1316
BssMI GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
BssNI GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
BssSI CACGAG 1 cut(s) 304
BssT1I CCWWGG 2 cut(s) 1122, 1316
Bst2BI CACGAG 1 cut(s) 304
Bst2UI CCWGG 3 cut(s) 235, 744, 1155
Bst4CI ACNGT 2 cut(s) 221, 571
Bst6I CTCTTC 2 cut(s) 418, 539
BstACI GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
BstBAI YACGTR 1 cut(s) 717
BstBI TTCGAA 1 cut(s) 24
BstDEI CTNAG 3 cut(s) 192, 792, 975
BstDSI CCRYGG 2 cut(s) 342, 1122
BstENI CCTNNNNNAGG 1 cut(s) 415
BstF5I GGATG 5 cut(s) 886, 929, 1075, 1133, 1194
BstFNI CGCG 4 cut(s) 344, 407, 770, 949
BstH2I RGCGCY 4 cut(s) 404, 530, 642, 777
BstHHI GCGC 6 cut(s) 403, 409, 529, 641, 770, 776
BstKTI GATC 6 cut(s) 429, 926, 947, 1366, 1399, 1462
BstMAI GTCTC 2 cut(s) 554, 976
BstMBI GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
BstMCI CGRYCG 1 cut(s) 1302
BstMWI GCNNNNNNNGC 7 cut(s) 118, 385, 645, 662, 851, 860, 1245
BstNI CCWGG 3 cut(s) 235, 744, 1155
BstNSI RCATGY 2 cut(s) 1345, 1447
BstPAI GACNNNNGTC 1 cut(s) 449
BstSCI CCNGG 3 cut(s) 233, 742, 1153
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 2 cut(s) 1423, 1568
BstUI CGCG 4 cut(s) 344, 407, 770, 949
BstV1I GCAGC 6 cut(s) 99, 397, 510, 940, 1357, 1522
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 1459
BstXI CCANNNNNNTGG 1 cut(s) 1183
BstYI RGATCY 1 cut(s) 1459
BstZI CGGCCG 1 cut(s) 1299
BtgI CCRYGG 2 cut(s) 342, 1122
BtsCI GGATG 5 cut(s) 886, 929, 1075, 1133, 1194
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 1 cut(s) 1389
BveI ACCTGC 1 cut(s) 382
CciNI GCGGCCGC 1 cut(s) 1299
CfoI GCGC 6 cut(s) 403, 409, 529, 641, 770, 776
Cfr10I RCCGGY 3 cut(s) 238, 259, 1277
Cfr42I CCGCGG 1 cut(s) 345
CseI GACGC 1 cut(s) 676
Csp6I GTAC 3 cut(s) 316, 1084, 1109
CviQI GTAC 3 cut(s) 316, 1084, 1109
DdeI CTNAG 3 cut(s) 192, 792, 975
DinI GGCGCC 2 cut(s) 402, 528
DpnI GATC 6 cut(s) 428, 925, 946, 1365, 1398, 1461
DpnII GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
EaeI YGGCCR 3 cut(s) 127, 1233, 1299
EagI CGGCCG 1 cut(s) 1299
Eam1104I CTCTTC 2 cut(s) 418, 539
EarI CTCTTC 2 cut(s) 418, 539
Ecl136II GAGCTC 2 cut(s) 379, 537
EclXI CGGCCG 1 cut(s) 1299
Eco130I CCWWGG 2 cut(s) 1122, 1316
Eco147I AGGCCT 1 cut(s) 508
Eco24I GRGCYC 3 cut(s) 381, 539, 1568
Eco47I GGWCC 8 cut(s) 346, 444, 451, 602, 961, 1138, 1313, 1481
Eco52I CGGCCG 1 cut(s) 1299
Eco53kI GAGCTC 2 cut(s) 379, 537
Eco57I CTGAAG 1 cut(s) 296
Eco72I CACGTG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 1415
EcoICRI GAGCTC 2 cut(s) 379, 537
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoO109I RGGNCCY 3 cut(s) 1313, 1564, 1565
EcoRII CCWGG 3 cut(s) 233, 742, 1153
EcoT14I CCWWGG 2 cut(s) 1122, 1316
EcoT38I GRGCYC 3 cut(s) 381, 539, 1568
EgeI GGCGCC 2 cut(s) 402, 528
EheI GGCGCC 2 cut(s) 402, 528
ErhI CCWWGG 2 cut(s) 1122, 1316
Esp3I CGTCTC 2 cut(s) 554, 976
FaqI GGGAC 4 cut(s) 700, 1124, 1326, 1467
FauI CCCGC 3 cut(s) 279, 327, 412
FauNDI CATATG 3 cut(s) 432, 1308, 1401
FblI GTMKAC 1 cut(s) 327
FokI GGATG 5 cut(s) 893, 916, 1082, 1140, 1201
FriOI GRGCYC 3 cut(s) 381, 539, 1568
FseI GGCCGGCC 1 cut(s) 242
FspBI CTAG 3 cut(s) 612, 627, 1317
GlaI GCGC 6 cut(s) 402, 408, 528, 640, 769, 775
GsaI CCCAGC 3 cut(s) 256, 687, 1534
GsuI CTGGAG 4 cut(s) 565, 736, 1082, 1176
HaeII RGCGCY 4 cut(s) 404, 530, 642, 777
HapII CCGG 8 cut(s) 92, 239, 260, 382, 518, 698, 820, 1278
HgaI GACGC 1 cut(s) 676
HhaI GCGC 6 cut(s) 403, 409, 529, 641, 770, 776
Hin1I GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
Hin6I GCGC 6 cut(s) 401, 407, 527, 639, 768, 774
HinP1I GCGC 6 cut(s) 401, 407, 527, 639, 768, 774
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 6 cut(s) 362, 514, 578, 623, 868, 893
HpaII CCGG 8 cut(s) 92, 239, 260, 382, 518, 698, 820, 1278
HphI GGTGA 2 cut(s) 223, 1275
Hpy166II GTNNAC 5 cut(s) 328, 577, 964, 1283, 1387
Hpy188I TCNGA 5 cut(s) 376, 795, 920, 976, 1051
Hpy188III TCNNGA 5 cut(s) 359, 454, 518, 989, 1577
Hpy8I GTNNAC 5 cut(s) 328, 577, 964, 1283, 1387
HpyAV CCTTC 2 cut(s) 346, 1006
HpyCH4III ACNGT 2 cut(s) 221, 571
HpyCH4IV ACGT 5 cut(s) 201, 689, 716, 734, 985
HpyCH4V TGCA 9 cut(s) 142, 286, 466, 956, 1041, 1345, 1358, 1457, 1495
HpyF10VI GCNNNNNNNGC 7 cut(s) 118, 385, 645, 662, 851, 860, 1245
HpyF3I CTNAG 3 cut(s) 192, 792, 975
HpySE526I ACGT 5 cut(s) 201, 689, 716, 734, 985
Hsp92I GRCGYC 7 cut(s) 201, 401, 527, 668, 689, 734, 985
HspAI GCGC 6 cut(s) 401, 407, 527, 639, 768, 774
KasI GGCGCC 2 cut(s) 400, 526
Kpn2I TCCGGA 1 cut(s) 517
KroI GCCGGC 2 cut(s) 238, 259
KroNI GCCGGC 2 cut(s) 240, 261
KspI CCGCGG 1 cut(s) 345
Kzo9I GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 4 cut(s) 109, 384, 662, 755
Lsp1109I GCAGC 6 cut(s) 99, 397, 510, 940, 1357, 1522
LweI GCATC 8 cut(s) 237, 850, 1028, 1067, 1118, 1245, 1248, 1482
MaeI CTAG 3 cut(s) 612, 627, 1317
MaeII ACGT 5 cut(s) 201, 689, 716, 734, 985
MaeIII GTNAC 3 cut(s) 709, 1101, 1497
MalI GATC 6 cut(s) 428, 925, 946, 1365, 1398, 1461
MbiI CCGCTC 1 cut(s) 322
MboI GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
MboII GAAGA 5 cut(s) 107, 302, 405, 553, 556
MfeI CAATTG 1 cut(s) 1077
MflI RGATCY 1 cut(s) 1459
MhlI GDGCHC 4 cut(s) 381, 539, 1423, 1568
MlsI TGGCCA 1 cut(s) 1235
MluCI AATT 3 cut(s) 1077, 1194, 1450
MluNI TGGCCA 1 cut(s) 1235
Mly113I GGCGCC 2 cut(s) 401, 527
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 2 cut(s) 701, 1029
Mox20I TGGCCA 1 cut(s) 1235
MroI TCCGGA 1 cut(s) 517
MroNI GCCGGC 2 cut(s) 238, 259
MscI TGGCCA 1 cut(s) 1235
MseI TTAA 1 cut(s) 1597
MslI CAYNNNNRTG 2 cut(s) 1181, 1211
Msp20I TGGCCA 1 cut(s) 1235
MspA1I CMGCKG 3 cut(s) 344, 595, 1298
MspI CCGG 8 cut(s) 92, 239, 260, 382, 518, 698, 820, 1278
MspR9I CCNGG 3 cut(s) 235, 744, 1155
MunI CAATTG 1 cut(s) 1077
MvaI CCWGG 3 cut(s) 235, 744, 1155
MvnI CGCG 4 cut(s) 344, 407, 770, 949
MwoI GCNNNNNNNGC 7 cut(s) 118, 385, 645, 662, 851, 860, 1245
NaeI GCCGGC 2 cut(s) 240, 261
NarI GGCGCC 2 cut(s) 401, 527
NcoI CCATGG 1 cut(s) 1122
NdeI CATATG 3 cut(s) 432, 1308, 1401
NdeII GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
NgoMIV GCCGGC 2 cut(s) 238, 259
NlaIV GGNNCC 9 cut(s) 402, 528, 603, 751, 1140, 1314, 1483, 1565, 1566
NmeAIII GCCGAG 4 cut(s) 462, 802, 889, 1575
NmuCI GTSAC 1 cut(s) 709
NotI GCGGCCGC 1 cut(s) 1299
NspI RCATGY 2 cut(s) 1345, 1447
NspV TTCGAA 1 cut(s) 24
PaeI GCATGC 1 cut(s) 1447
PceI AGGCCT 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 5 cut(s) 30, 132, 210, 422, 734
PdiI GCCGGC 2 cut(s) 240, 261
PfeI GAWTC 5 cut(s) 362, 514, 623, 868, 893
PflFI GACNNNGTC 2 cut(s) 101, 736
PflMI CCANNNNNTGG 2 cut(s) 683, 1541
PfoI TCCNGGA 1 cut(s) 1153
PleI GAGTC 1 cut(s) 572
PluTI GGCGCC 2 cut(s) 404, 530
PmaCI CACGTG 1 cut(s) 717
PmlI CACGTG 1 cut(s) 717
PpsI GAGTC 1 cut(s) 572
Ppu21I YACGTR 1 cut(s) 717
PpuMI RGGWCCY 1 cut(s) 1313
PshAI GACNNNNGTC 1 cut(s) 449
Psp124BI GAGCTC 2 cut(s) 381, 539
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 3 cut(s) 233, 742, 1153
PspCI CACGTG 1 cut(s) 717
PspFI CCCAGC 3 cut(s) 252, 683, 1530
PspGI CCWGG 3 cut(s) 233, 742, 1153
PspN4I GGNNCC 9 cut(s) 402, 528, 603, 751, 1140, 1314, 1483, 1565, 1566
PspOMI GGGCCC 1 cut(s) 1564
PspPPI RGGWCCY 1 cut(s) 1313
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 1459
PsyI GACNNNGTC 2 cut(s) 101, 736
RigI GGCCGGCC 1 cut(s) 242
RsaI GTAC 3 cut(s) 317, 1085, 1110
RsaNI GTAC 3 cut(s) 316, 1084, 1109
RseI CAYNNNNRTG 2 cut(s) 1181, 1211
SacI GAGCTC 2 cut(s) 381, 539
SacII CCGCGG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 1 cut(s) 1597
Sau3AI GATC 6 cut(s) 426, 923, 944, 1363, 1396, 1459
ScaI AGTACT 1 cut(s) 1085
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 235, 744, 1155
SduI GDGCHC 4 cut(s) 381, 539, 1423, 1568
SfaNI GCATC 8 cut(s) 237, 850, 1028, 1067, 1118, 1245, 1248, 1482
SfcI CTRYAG 1 cut(s) 954
SfoI GGCGCC 2 cut(s) 402, 528
Sfr303I CCGCGG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 345
SgrDI CGTCGACG 1 cut(s) 326
SinI GGWCC 8 cut(s) 346, 444, 451, 602, 961, 1138, 1313, 1481
SmiMI CAYNNNNRTG 2 cut(s) 1181, 1211
SphI GCATGC 1 cut(s) 1447
Sse9I AATT 3 cut(s) 1077, 1194, 1450
SseBI AGGCCT 1 cut(s) 508
SspDI GGCGCC 2 cut(s) 400, 526
SspMI CTAG 3 cut(s) 612, 627, 1317
SstI GAGCTC 2 cut(s) 381, 539
StuI AGGCCT 1 cut(s) 508
StyD4I CCNGG 3 cut(s) 233, 742, 1153
StyI CCWWGG 2 cut(s) 1122, 1316
TaaI ACNGT 2 cut(s) 221, 571
TaiI ACGT 5 cut(s) 204, 692, 719, 737, 988
TaqI TCGA 8 cut(s) 24, 327, 416, 533, 539, 926, 988, 1036
TaqII GACCGA 2 cut(s) 461, 1469
TasI AATT 3 cut(s) 1077, 1194, 1450
TatI WGTACW 2 cut(s) 1083, 1108
TfiI GAWTC 5 cut(s) 362, 514, 623, 868, 893
Tru1I TTAA 1 cut(s) 1597
Tru9I TTAA 1 cut(s) 1597
TscAI CASTG 1 cut(s) 1389
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 6 cut(s) 112, 385, 498, 953, 1345, 1510
Tsp45I GTSAC 1 cut(s) 709
TspDTI ATGAA 5 cut(s) 17, 309, 1017, 1025, 1086
TspRI CASTG 1 cut(s) 1389
Tth111I GACNNNGTC 2 cut(s) 101, 736
Van91I CCANNNNNTGG 2 cut(s) 683, 1541
VpaK11BI GGWCC 8 cut(s) 346, 444, 451, 602, 961, 1138, 1313, 1481
XagI CCTNNNNNAGG 1 cut(s) 415
XceI RCATGY 2 cut(s) 1345, 1447
XcmI CCANNNNNNNNNTGG 1 cut(s) 1538
XmaJI CCTAGG 1 cut(s) 1316
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 3 cut(s) 612, 627, 1317
ZraI GACGTC 4 cut(s) 202, 690, 735, 986
ZrmI AGTACT 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.