pycom10g16200

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004582
Physical Location & Seq
Forward (+)
19387872 .. 19388395
524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGCAGAGCCGCGCTTACGATCGATCATATGAAGCTCAAGCTAGGGTTCAAGATCCTATTTATGGCTGTGTTTCCCATATTTTTTCTCTCCAACAACAGATTATTAGTTTAGAAGCACAACTAGCTTCAGTCAAGGAACAAGCAGCTAAAGGCTATTCCAGCAATAATTTCCAAGGTTGGCCTCAGTCGGAAAATTCAAACACATTGATGCCACAAAGTGCGATTAATGAATCAATGACACCAGTATATTCCGGAAACAAGAACCCTAACTCTTTCGGGGCTAACGAAAATTTCATGATTCGAGGAGTACTAAATGACATGTACGGAAGCTACGGAGAGTATTATAATTCCATGTGTTCATTAGACATGCAAATGAGCAATATTTCATGGGGTTTTGATCAAGATCATGACTGA

Protein Analysis

138

Amino Acids

15.45

Weight (kDa)

4.58

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 10 - 45 5e-08 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 251
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 47
AcsI RAATTY 2 cut(s) 193, 289
AcuI CTGAAG 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 2 cut(s) 309, 323
AfiI CCNNNNNNNGG 1 cut(s) 62
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 2 cut(s) 50, 198
AluBI AGCT 5 cut(s) 35, 41, 125, 146, 330
AluI AGCT 5 cut(s) 35, 41, 125, 146, 330
AlwI GGATC 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 251
AoxI GGCC 1 cut(s) 179
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 2 cut(s) 193, 289
AseI ATTAAT 1 cut(s) 225
AspLEI GCGC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 155
BclI TGATCA 1 cut(s) 397
BfaI CTAG 2 cut(s) 42, 122
BisI GCNGC 2 cut(s) 10, 144
BlsI GCNGC 2 cut(s) 11, 145
BmcAI AGTACT 1 cut(s) 309
BmsI GCATC 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 21
Bsa29I ATCGAT 1 cut(s) 22
BsaBI GATNNNNATC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 242
Bse8I GATNNNNATC 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 251
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 172
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 155
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 1 cut(s) 181
BshVI ATCGAT 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 22
BsiSI CCGG 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 62
BsnI GGCC 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 251
Bsp143I GATC 5 cut(s) 19, 23, 52, 397, 403
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 181
BspCNI CTCAG 1 cut(s) 196
BspDI ATCGAT 1 cut(s) 22
BspEI TCCGGA 1 cut(s) 251
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 2 cut(s) 294, 406
BspPI GGATC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 5 cut(s) 19, 23, 52, 397, 403
BssT1I CCWWGG 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 183
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 5 cut(s) 22, 26, 55, 400, 406
BstMBI GATC 5 cut(s) 19, 23, 52, 397, 403
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 2 cut(s) 122, 159
BstNSI RCATGY 2 cut(s) 322, 370
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 22
BsuRI GGCC 1 cut(s) 181
BsuTUI ATCGAT 1 cut(s) 22
CciI TCATGA 2 cut(s) 294, 406
CfoI GCGC 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 22
Csp6I GTAC 2 cut(s) 308, 322
CviAII CATG 6 cut(s) 295, 319, 352, 367, 387, 407
CviQI GTAC 2 cut(s) 308, 322
DdeI CTNAG 1 cut(s) 183
DpnI GATC 5 cut(s) 21, 25, 54, 399, 405
DpnII GATC 5 cut(s) 19, 23, 52, 397, 403
DraIII CACNNNGTG 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 6 cut(s) 298, 322, 355, 370, 390, 410
FatI CATG 6 cut(s) 294, 318, 351, 366, 386, 406
FauNDI CATATG 1 cut(s) 28
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 10, 144
Fsp4HI GCNGC 2 cut(s) 10, 144
FspBI CTAG 2 cut(s) 42, 122
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 10, 144
HaeIII GGCC 1 cut(s) 181
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 14
Hin1II CATG 6 cut(s) 298, 322, 355, 370, 390, 410
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HinfI GANTC 2 cut(s) 230, 298
HpaII CCGG 1 cut(s) 252
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 5 cut(s) 50, 252, 295, 401, 407
HpyCH4V TGCA 2 cut(s) 4, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 159
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 6 cut(s) 298, 322, 355, 370, 390, 410
HspAI GCGC 1 cut(s) 12
Kpn2I TCCGGA 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 5 cut(s) 19, 23, 52, 397, 403
LpnPI CCDG 3 cut(s) 172, 255, 265
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 198
MaeI CTAG 2 cut(s) 42, 122
MalI GATC 5 cut(s) 21, 25, 54, 399, 405
MboI GATC 5 cut(s) 19, 23, 52, 397, 403
MflI RGATCY 1 cut(s) 52
MluCI AATT 4 cut(s) 166, 193, 289, 346
MmeI TCCRAC 2 cut(s) 115, 168
MnlI CCTC 2 cut(s) 192, 296
MroI TCCGGA 1 cut(s) 251
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 206, 371
MspI CCGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 122, 159
NdeI CATATG 1 cut(s) 28
NdeII GATC 5 cut(s) 19, 23, 52, 397, 403
NlaIII CATG 6 cut(s) 298, 322, 355, 370, 390, 410
NspI RCATGY 2 cut(s) 322, 370
PagI TCATGA 2 cut(s) 294, 406
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PfeI GAWTC 2 cut(s) 230, 298
PkrI GCNGC 2 cut(s) 11, 145
Ple19I CGATCG 1 cut(s) 22
PscI ACATGT 1 cut(s) 318
PshBI ATTAAT 1 cut(s) 225
PsiI TTATAA 1 cut(s) 345
PsuI RGATCY 1 cut(s) 52
PvuI CGATCG 1 cut(s) 22
RsaI GTAC 2 cut(s) 309, 323
RsaNI GTAC 2 cut(s) 308, 322
RseI CAYNNNNRTG 2 cut(s) 206, 371
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 10, 144
Sau3AI GATC 5 cut(s) 19, 23, 52, 397, 403
ScaI AGTACT 1 cut(s) 309
SetI ASST 6 cut(s) 37, 43, 127, 148, 178, 332
SfaNI GCATC 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 206, 371
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
Sse9I AATT 4 cut(s) 166, 193, 289, 346
SsiI CCGC 1 cut(s) 10
SspI AATATT 1 cut(s) 382
SspMI CTAG 2 cut(s) 42, 122
StyI CCWWGG 1 cut(s) 172
TaqI TCGA 2 cut(s) 22, 301
TasI AATT 4 cut(s) 166, 193, 289, 346
TatI WGTACW 1 cut(s) 307
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 2 cut(s) 230, 298
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 5 cut(s) 45, 243, 283, 348, 375
TspGWI ACGGA 2 cut(s) 339, 348
VspI ATTAAT 1 cut(s) 225
XapI RAATTY 2 cut(s) 193, 289
XceI RCATGY 2 cut(s) 322, 370
XspI CTAG 2 cut(s) 42, 122
ZrmI AGTACT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.