pycom10g16440

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009506
Physical Location & Seq
Forward (+)
19670973 .. 19672185
1213 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGCATCACTGCACCGTAACCCTGAAACCCCCACCTCCACACCATGTATCCTCCTCTCGCTCCCACCTGATTTTTCCCCCCAGCCCTAGGCTGACCAAATCCCAATCCCACCCCATCTTCACGCCAATTTACATTCGTGCTCATTCCGTCTCCAACCGCACCGCCAGAGGGCTCGATGTCTCCGTCTCCAAACACTCCCCAAACGCCAGCGCCCCAAATGGCTCCCTACACGATTGGAAGCCGAAGAACGTCGGCGTTAAAGACGTAGATGTCGCCACTCTCGGCAATCTCTGCGTGGATATCGTGCTCAATGTGCCCAAATTGCCACCGCCCAATGTCCACGACCGCAAATCTTACATGGACCGGTTGTCCGCCTCGCCGCCGGATAAGCAATACTGGGAAGCTGGTGGAAACTGCAATATGGCCATAGCAGCTGCAAGATTAGGACTTAAGTGCATCGCAATTGGGCACGTGGGTAACGAAGTATATGGACAGTTCCTTTTAGATGTCCTTCAAGATGAAGGAATTAGCATGGTTGGAGTGAGTGACAACAATACCAATGTTGACAGTTCAAGTGCAACGTATGAAACACTTTTATGCTGGGTACTCGTCGATCCATTGCAAAGACATGGTTTTTCCGAGCTGATTTCAGTAAGGAGCCTGCATTCAGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

226

Amino Acids

24.44

Weight (kDa)

8.29

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 127 - 184 1.1e-07 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AciI CCGC 6 cut(s) 157, 162, 329, 346, 372, 380
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 423
AcvI CACGTG 1 cut(s) 472
AfaI GTAC 1 cut(s) 606
AfiI CCNNNNNNNGG 2 cut(s) 87, 168
AflII CTTAAG 1 cut(s) 449
AgeI ACCGGT 1 cut(s) 363
AgsI TTSAA 2 cut(s) 515, 573
AhdI GACNNNNNGTC 1 cut(s) 367
AluBI AGCT 3 cut(s) 404, 434, 643
AluI AGCT 3 cut(s) 404, 434, 643
Alw21I GWGCWC 2 cut(s) 142, 309
Alw26I GTCTC 3 cut(s) 154, 184, 190
AlwI GGATC 1 cut(s) 608
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 2 cut(s) 431, 434
AsiGI ACCGGT 1 cut(s) 363
AspA2I CCTAGG 1 cut(s) 86
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 361
AvaII GGWCC 1 cut(s) 361
AvrII CCTAGG 1 cut(s) 86
BaeGI GKGCMC 2 cut(s) 318, 471
BalI TGGCCA 1 cut(s) 425
BanII GRGCYC 1 cut(s) 174
BbrPI CACGTG 1 cut(s) 472
Bbv12I GWGCWC 2 cut(s) 142, 309
BbvI GCAGC 2 cut(s) 421, 443
BccI CCATC 1 cut(s) 122
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 154, 184, 190
BfaI CTAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 449
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 3 cut(s) 380, 432, 435
BlnI CCTAGG 1 cut(s) 86
BlsI GCNGC 3 cut(s) 381, 433, 436
Bme18I GGWCC 1 cut(s) 361
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 2 cut(s) 223, 659
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 13, 465
BmuI ACTGGG 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 86
BsaWI WCCGGW 1 cut(s) 363
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 168
Bse118I RCCGGY 1 cut(s) 363
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 617
BseDI CCNNGG 1 cut(s) 86
BseLI CCNNNNNNNGG 2 cut(s) 87, 168
BseMI GCAATG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 43
BseSI GKGCMC 2 cut(s) 318, 471
BseXI GCAGC 2 cut(s) 421, 443
BseYI CCCAGC 2 cut(s) 80, 600
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 363
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 2 cut(s) 142, 309
BsiSI CCGG 2 cut(s) 364, 383
BslI CCNNNNNNNGG 2 cut(s) 87, 168
BsmAI GTCTC 3 cut(s) 154, 184, 190
BsmBI CGTCTC 2 cut(s) 154, 190
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 1 cut(s) 425
Bsp1286I GDGCHC 5 cut(s) 142, 174, 309, 318, 471
Bsp143I GATC 1 cut(s) 613
BspACI CCGC 6 cut(s) 157, 162, 329, 346, 372, 380
BspANI GGCC 1 cut(s) 425
BspLI GGNNCC 2 cut(s) 223, 659
BspPI GGATC 1 cut(s) 608
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 617
BsrFI RCCGGY 1 cut(s) 363
BsrI ACTGG 1 cut(s) 401
BssAI RCCGGY 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 1 cut(s) 613
BssT1I CCWWGG 1 cut(s) 86
Bst4CI ACNGT 3 cut(s) 16, 495, 569
BstAFI CTTAAG 1 cut(s) 449
BstAPI GCANNNNNTGC 1 cut(s) 291
BstBAI YACGTR 1 cut(s) 472
BstC8I GCNNGC 2 cut(s) 208, 662
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 1 cut(s) 616
BstMAI GTCTC 3 cut(s) 154, 184, 190
BstMBI GATC 1 cut(s) 613
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 5 cut(s) 291, 313, 322, 388, 431
BstSLI GKGCMC 2 cut(s) 318, 471
BstV1I GCAGC 2 cut(s) 421, 443
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 425
BtgZI GCGATG 1 cut(s) 442
BtsI GCAGTG 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 7
Cac8I GCNNGC 2 cut(s) 208, 662
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 361
Csp6I GTAC 1 cut(s) 605
CspAI ACCGGT 1 cut(s) 363
CviAII CATG 4 cut(s) 44, 358, 532, 629
CviQI GTAC 1 cut(s) 605
DpnI GATC 1 cut(s) 615
DpnII GATC 1 cut(s) 613
DrdI GACNNNNNNGTC 1 cut(s) 269
DriI GACNNNNNGTC 1 cut(s) 367
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 367
EciI GGCGGA 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 174
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 361
Eco72I CACGTG 1 cut(s) 472
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 2 cut(s) 154, 190
FaeI CATG 4 cut(s) 47, 361, 535, 632
FatI CATG 4 cut(s) 43, 357, 531, 628
Fnu4HI GCNGC 3 cut(s) 380, 432, 435
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 3 cut(s) 380, 432, 435
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 380, 432, 435
GsaI CCCAGC 2 cut(s) 84, 604
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 425
HapII CCGG 2 cut(s) 364, 383
HhaI GCGC 1 cut(s) 212
Hin1II CATG 4 cut(s) 47, 361, 535, 632
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HpaII CCGG 2 cut(s) 364, 383
Hpy166II GTNNAC 2 cut(s) 340, 565
Hpy188I TCNGA 1 cut(s) 640
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 2 cut(s) 340, 565
Hpy99I CGWCG 2 cut(s) 254, 614
HpyAV CCTTC 2 cut(s) 515, 521
HpyCH4III ACNGT 3 cut(s) 16, 495, 569
HpyCH4IV ACGT 4 cut(s) 249, 264, 471, 581
HpyCH4V TGCA 8 cut(s) 4, 12, 417, 437, 456, 578, 622, 664
HpyF10VI GCNNNNNNNGC 5 cut(s) 291, 313, 322, 388, 431
HpySE526I ACGT 4 cut(s) 249, 264, 471, 581
Hsp92II CATG 4 cut(s) 47, 361, 535, 632
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 1 cut(s) 613
LmnI GCTCC 3 cut(s) 65, 227, 657
Lsp1109I GCAGC 2 cut(s) 421, 443
LweI GCATC 2 cut(s) 13, 465
MaeI CTAG 1 cut(s) 87
MaeII ACGT 4 cut(s) 249, 264, 471, 581
MaeIII GTNAC 3 cut(s) 16, 476, 545
MalI GATC 1 cut(s) 615
MboI GATC 1 cut(s) 613
MboII GAAGA 2 cut(s) 109, 256
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 5 cut(s) 142, 174, 309, 318, 471
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 4 cut(s) 126, 320, 462, 525
MluNI TGGCCA 1 cut(s) 425
MmeI TCCRAC 3 cut(s) 177, 517, 652
MnlI CCTC 5 cut(s) 45, 61, 64, 161, 385
Mox20I TGGCCA 1 cut(s) 425
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 425
MseI TTAA 2 cut(s) 258, 450
MslI CAYNNNNRTG 1 cut(s) 595
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 364, 383
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 664
MwoI GCNNNNNNNGC 5 cut(s) 291, 313, 322, 388, 431
NdeII GATC 1 cut(s) 613
NlaIII CATG 4 cut(s) 47, 361, 535, 632
NlaIV GGNNCC 2 cut(s) 223, 659
NmeAIII GCCGAG 1 cut(s) 261
NmuCI GTSAC 1 cut(s) 545
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 477
PctI GAATGC 1 cut(s) 664
PinAI ACCGGT 1 cut(s) 363
PkrI GCNGC 3 cut(s) 381, 433, 436
PmaCI CACGTG 1 cut(s) 472
PmlI CACGTG 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 472
PspCI CACGTG 1 cut(s) 472
PspFI CCCAGC 2 cut(s) 80, 600
PspN4I GGNNCC 2 cut(s) 223, 659
PspPI GGNCC 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
RseI CAYNNNNRTG 1 cut(s) 595
SaqAI TTAA 2 cut(s) 258, 450
SatI GCNGC 3 cut(s) 380, 432, 435
Sau3AI GATC 1 cut(s) 613
Sau96I GGNCC 1 cut(s) 361
SduI GDGCHC 5 cut(s) 142, 174, 309, 318, 471
SetI ASST 9 cut(s) 37, 69, 252, 267, 406, 436, 474, 584, 645
SfaNI GCATC 2 cut(s) 13, 465
SinI GGWCC 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 4 cut(s) 126, 320, 462, 525
SsiI CCGC 6 cut(s) 157, 162, 329, 346, 372, 380
SspMI CTAG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 3 cut(s) 16, 495, 569
TaiI ACGT 4 cut(s) 252, 267, 474, 584
TaqI TCGA 2 cut(s) 174, 612
TasI AATT 4 cut(s) 126, 320, 462, 525
TauI GCSGC 1 cut(s) 382
Tru1I TTAA 2 cut(s) 258, 450
Tru9I TTAA 2 cut(s) 258, 450
TscAI CASTG 1 cut(s) 14
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 2 cut(s) 431, 434
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 2 cut(s) 534, 600
TspGWI ACGGA 2 cut(s) 136, 172
TspRI CASTG 1 cut(s) 14
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 361
XmaJI CCTAGG 1 cut(s) 86
XspI CTAG 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.