pycom10g16450

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009506
Physical Location & Seq
Forward (+)
19672404 .. 19673625
1222 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGAGTGACGTTCTTCTCCTAACATCTGATGAGGCTGAGTCATTGACGAGCATTAAAAACCCAATATTAGCAGGGCAGGAGCTGCTCAAGCAAGGGGTGCACACAAAATGGGTGATTGTCAAAATGGGTCCAAGGGGTTCAATTCTAATAACTAGGTCAAGTATTTCTTTCGCACCAGCTTTTGAGGTAAATGTCGTAGATACTGTTGGATGTGGAGATAGTTTCGTGGCTGCGATTGCATATGGTTTTATCCACAACATGCCGATGGTGAACACATTAGCAATTGCAAATGCAGTAGGTGCTGCAACCGCCACAGGTTGTGGTGCTGGTAGGAATGTGGCAACCTTGGATAAAGTAATAGAACTTATAAGGGCATCTAATCTCAAGGAGGATGACAAATTTTGGAATGAGTTAGTTGATGAAAATTTGGGTGTCGAGGAAATTACATCGCTTTCAAATTTGGTCATCAATGGAAGTGATAACCGGTTAAATTGTGTATCCTTGCAGAAGGTGGTATCCGAATTGCTTCCTAAGCTCAAATCTGCACAGTTAGAAAGAAAAGTGTCTTTTTCTGGAGCACGATCGATCATAACAACGGTAGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PfkB

Protein Analysis

203

Amino Acids

21.53

Weight (kDa)

5.44

Isoelectric Point (pI)

27.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 2 - 111 1.1e-22 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AciI CCGC 1 cut(s) 309
AcsI RAATTY 3 cut(s) 398, 424, 457
AgeI ACCGGT 1 cut(s) 483
AgsI TTSAA 2 cut(s) 141, 456
AluBI AGCT 3 cut(s) 82, 179, 535
AluI AGCT 3 cut(s) 82, 179, 535
Alw21I GWGCWC 2 cut(s) 102, 580
Alw44I GTGCAC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 82
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 3 cut(s) 82, 230, 302
ApoI RAATTY 3 cut(s) 398, 424, 457
AsiGI ACCGGT 1 cut(s) 483
Asp700I GAANNNNTTC 1 cut(s) 525
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 124, 280
AvaII GGWCC 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 102, 580
BbvI GCAGC 3 cut(s) 69, 217, 289
BccI CCATC 1 cut(s) 259
BciVI GTATCC 2 cut(s) 508, 526
BfaI CTAG 1 cut(s) 153
BfuI GTATCC 2 cut(s) 508, 526
BisI GCNGC 3 cut(s) 83, 231, 303
BlsI GCNGC 3 cut(s) 84, 232, 304
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 129
BmsI GCATC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 71, 368
Bsa29I ATCGAT 1 cut(s) 584
BsaJI CCNNGG 2 cut(s) 131, 345
BsaWI WCCGGW 1 cut(s) 483
Bse118I RCCGGY 1 cut(s) 483
BseCI ATCGAT 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 131, 345
BseGI GGATG 2 cut(s) 215, 397
BseMII CTCAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 3 cut(s) 69, 217, 289
BsgI GTGCAG 1 cut(s) 528
Bsh1285I CGRYCG 1 cut(s) 584
BshTI ACCGGT 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 584
BsiEI CGRYCG 1 cut(s) 584
BsiHKAI GWGCWC 2 cut(s) 102, 580
BsiSI CCGG 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 102, 580
Bsp143I GATC 2 cut(s) 581, 585
BspACI CCGC 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 28
BspDI ATCGAT 1 cut(s) 584
BspLI GGNNCC 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 483
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 2 cut(s) 131, 345
BssMI GATC 2 cut(s) 581, 585
BssT1I CCWWGG 2 cut(s) 131, 345
Bst4CI ACNGT 3 cut(s) 205, 549, 598
BstAPI GCANNNNNTGC 3 cut(s) 82, 97, 299
BstDEI CTNAG 2 cut(s) 36, 531
BstF5I GGATG 2 cut(s) 215, 397
BstKTI GATC 2 cut(s) 584, 588
BstMBI GATC 2 cut(s) 581, 585
BstMCI CGRYCG 1 cut(s) 584
BstMWI GCNNNNNNNGC 7 cut(s) 82, 88, 97, 236, 299, 308, 532
BstNSI RCATGY 1 cut(s) 262
BstSLI GKGCMC 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 69, 217, 289
Bsu15I ATCGAT 1 cut(s) 584
BsuI GTATCC 2 cut(s) 508, 526
BsuTUI ATCGAT 1 cut(s) 584
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 215, 397
CaiI CAGNNNCTG 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 483
Cfr13I GGNCC 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 584
CspAI ACCGGT 1 cut(s) 483
CviAII CATG 1 cut(s) 259
CviJI RGCY 5 cut(s) 35, 82, 179, 230, 535
CviKI_1 RGCY 5 cut(s) 35, 82, 179, 230, 535
DdeI CTNAG 2 cut(s) 36, 531
DpnI GATC 2 cut(s) 583, 587
DpnII GATC 2 cut(s) 581, 585
Eco130I CCWWGG 2 cut(s) 131, 345
Eco47I GGWCC 1 cut(s) 128
EcoT14I CCWWGG 2 cut(s) 131, 345
ErhI CCWWGG 2 cut(s) 131, 345
FaeI CATG 1 cut(s) 262
FaiI YATR 5 cut(s) 241, 243, 260, 368, 590
FalI AAGNNNNNCTT 2 cut(s) 151, 183
FatI CATG 1 cut(s) 258
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 3 cut(s) 83, 231, 303
FokI GGATG 2 cut(s) 222, 404
Fsp4HI GCNGC 3 cut(s) 83, 231, 303
FspBI CTAG 1 cut(s) 153
GluI GCNGC 3 cut(s) 83, 231, 303
GsuI CTGGAG 1 cut(s) 594
HapII CCGG 1 cut(s) 484
Hin1II CATG 1 cut(s) 262
HinfI GANTC 1 cut(s) 38
HpaII CCGG 1 cut(s) 484
HphI GGTGA 2 cut(s) 124, 280
Hpy166II GTNNAC 2 cut(s) 100, 271
Hpy188I TCNGA 2 cut(s) 28, 520
Hpy188III TCNNGA 1 cut(s) 573
Hpy8I GTNNAC 2 cut(s) 100, 271
HpyAV CCTTC 1 cut(s) 502
HpyCH4III ACNGT 3 cut(s) 205, 549, 598
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 7 cut(s) 100, 239, 287, 293, 305, 505, 545
HpyF10VI GCNNNNNNNGC 7 cut(s) 82, 88, 97, 236, 299, 308, 532
HpyF3I CTNAG 2 cut(s) 36, 531
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 1 cut(s) 262
Kzo9I GATC 2 cut(s) 581, 585
LmnI GCTCC 2 cut(s) 79, 575
LpnPI CCDG 7 cut(s) 57, 62, 189, 300, 312, 497, 558
Lsp1109I GCAGC 3 cut(s) 69, 217, 289
LweI GCATC 1 cut(s) 383
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 5
MalI GATC 2 cut(s) 583, 587
MboI GATC 2 cut(s) 581, 585
MboII GAAGA 1 cut(s) 5
MfeI CAATTG 1 cut(s) 282
MhlI GDGCHC 2 cut(s) 102, 580
MluCI AATT 8 cut(s) 141, 282, 398, 424, 441, 457, 490, 521
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 4 cut(s) 25, 178, 382, 430
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 2 cut(s) 54, 488
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 1 cut(s) 484
MunI CAATTG 1 cut(s) 282
MwoI GCNNNNNNNGC 7 cut(s) 82, 88, 97, 236, 299, 308, 532
NdeI CATATG 1 cut(s) 241
NdeII GATC 2 cut(s) 581, 585
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 5
NspI RCATGY 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 525
PinAI ACCGGT 1 cut(s) 483
PkrI GCNGC 3 cut(s) 84, 232, 304
Ple19I CGATCG 1 cut(s) 584
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 82
PvuI CGATCG 1 cut(s) 584
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 2 cut(s) 54, 488
SatI GCNGC 3 cut(s) 83, 231, 303
Sau3AI GATC 2 cut(s) 581, 585
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 47
SduI GDGCHC 2 cut(s) 102, 580
SfaNI GCATC 1 cut(s) 383
SinI GGWCC 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 2 cut(s) 86, 383
SmoI CTYRAG 2 cut(s) 86, 383
Sse9I AATT 8 cut(s) 141, 282, 398, 424, 441, 457, 490, 521
SsiI CCGC 1 cut(s) 309
SspI AATATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 153
StyI CCWWGG 2 cut(s) 131, 345
TaaI ACNGT 3 cut(s) 205, 549, 598
TaiI ACGT 1 cut(s) 12
TaqI TCGA 2 cut(s) 435, 584
TasI AATT 8 cut(s) 141, 282, 398, 424, 441, 457, 490, 521
Tru1I TTAA 2 cut(s) 54, 488
Tru9I TTAA 2 cut(s) 54, 488
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 3 cut(s) 82, 230, 302
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 435
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 3 cut(s) 398, 424, 457
XceI RCATGY 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.