Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0008257

Physical Location & Seq

Genomic Coordinates Reverse (-)

22588025 .. 22591207

Gene Structure 3183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGTTTTGTCCTTACCTACCTGCATGCCATCAAACACTATCTTCTTCTCACAAACTGGAACGCTCCTCCTCCTCCAATTTCCAAACATTCATGTTGATCGCTGAACGCGCCATTAGTTATATTTTTGTTGCGCCAAGAAGCTGCAAGAGTGATGCAAGATGGAAAGCCAAGAGTTCCAGTAGTAGAGCTGTTGAGAAGAGCAAGGGCGACGGACTTTACATTGACAAACGCGGAAAATTCAGAAGCTTCAATAACAAGAAACTGTCTAGGAAACGATGTGGTTCTCTAAGGGGCCGGGGATGGAAATATGGATCGGGGTTTGTGGACGGAGTTTTCCCGGTGATGAGCCCCACTGGTCAGCAGATTCTGGACTTTGTACCGAAGGAAGTCGATCAAAACAGTATTTGGGAAATTCTTGACACCCTTCCAGCCTCTCACACCATATGGGACGATATCATTAACGTAGCTGTTCAGCTTCGTCTCAACAAACAGTGGGGTTCCATAATCTTGATATGTGAGTGGATATTGTACAAGAGCTCCTTCAAGCCAGATGTCATCTGCTACAATTTGCTTATCGACGCTTATGGGCAGAAGTCGCAACATAAAGATGCAGAATCGACATATTTGGAACTTCTTGAAGCTCGATGCGTACCTACTGAAGACACCTATGCTCTTCTTTTAAAGGCCTTCTGCAAGTCTGGGTTGTTCGATAAGGCTGAAGCCATCTTTGGCGAAATGCGGAAGTATGGTCTTCCACCAAGTGCAATCGTGTTCGATGCTTACATTAACGGGTTACTAAAAGGCGGAAACCCTCAAAAAGCAGTAGAGATCTTTCAGAGGATGAAGAGAGATCAATGCCAGCCATCTACTGAGACGTATACGATGCTAATCAATTTATACGGAAGGGAAAGGAAATCTTTTATGGCTCTAAAGCTGTTTCAAGAAATGAAAAGCCAGAAATGTAGACCCAACATATGCACGTATACAGCTTTGGTAAATGCATTTGCGAGGGACGGACTTTGTGAGAAAGCGGAAGAAATTTTTGAGCAGATGCAAGAAGCAGGGTATGAGCCTGATGTGTATGCGTACAATGCCCTTATGGAAGCTTACAGTCGTGCAGGGTTTCCTTACGGAGCTGCAGAAATTTTCTCTCTAATGCAGCACATGGGATGTGAACCCGATAGAGCTTCGTATAATATCATGGTAGATGCATATGGGAGAGCTGGTCTTCATGAAGATGCACAAGCTGCGTTTGAAGAGATGAAGCGGTTAGGAATAACCCCGACAGTGAAATCCCATATGCTACTTTTATCCGCCTACTCCAAAGCTGGCAACGTATCAAAATGCGAGGACATTGTGAACCAGATGCAAGAGTCCGGGTTGGAGCTAGACACTTTCGTCATTAACAGCATGTTAAATCTGTATGGCCGGTTAGGCCAGTTAGAAAAGATGGAAGAAGTTTTGACAGCAATGGAAACAGGACCCTACGCAGCTGATATCAGTACATACAACATCTTGATTAACATATATGGACGGGCAGGATTTTTCGACAAGATGGAAGAGCTGTTTCAGTCGCTTCCACTCAAAAACTTGAAACCGGATGTTGTGACATGGACTTCGCGTCTTGGAGCATACTCGAGAAAGAAACTATACACGAGATGCTTGGAAATTTTCGAAGAAATGATTGATGCCGGTTGCTATCCAGATGCCGGAACTGCCAGGGTACTTCTTGGAGCGTGTTCAAGCGACGATCAAACCGAGCAGGTTAGTACTGTTATTAGAACTATGCACAAGGAGGTCAGGACTGTTTTACCTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

607

Amino Acids

68.51

Weight (kDa)

7.42

Isoelectric Point (pI)

42.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	180 - 231	5e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	183 - 232	1.2e-10	PPR repeat family
PPR_1	PF12854	216 - 247	6.9e-07	PPR repeat
PPR	PF01535	222 - 251	1.9e-06	PPR repeat
PPR_3	PF13812	242 - 302	6.1e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	253 - 299	4.7e-10	PPR repeat family
PPR_long	PF17177	272 - 374	7.1e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	290 - 337	9.5e-13	PPR repeat family
PPR	PF01535	292 - 321	3.9e-06	PPR repeat
PPR_3	PF13812	292 - 335	9.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	321 - 352	5.1e-08	PPR repeat
PPR_2	PF13041	323 - 371	5.8e-12	PPR repeat family
PPR	PF01535	327 - 355	9.3e-08	PPR repeat
PPR_3	PF13812	347 - 406	1.4e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	358 - 405	6.4e-11	PPR repeat family
TPR_24	PF23276	371 - 484	6e-08	Fungal tetratrico peptide repeats
PPR_long	PF17177	379 - 493	2.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	389 - 422	7e-08	PPR repeat
PPR_2	PF13041	396 - 438	1.7e-07	PPR repeat family
PPR	PF01535	397 - 426	1e-07	PPR repeat
PPR_2	PF13041	499 - 540	2.9e-07	PPR repeat family
PPR_2	PF13041	533 - 581	2.2e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35130 AT2G35130 AT2G35130
fragaria_vesca	FvH4_3g12360
malus_domestica	MD05G1251300.v1.1 MD10G1231700.v1.1 MD10G1231800.v1.1
prunus_persica	Prupe.4G112600_v2.0.a1
pyrus_communis	pycom05g20750 pycom05g22900 pycom10g19500 pycom10g19510
rosa_chinensis	RchiOBHm_Chr5g0020151
rosa_laevigata	RLG00000032505
rosa_roxburghii	Rroxscaffold_1G00058050
rosa_rugosa	Rorug05G0057400 Rorug05G0057500
rosa_samantha	Rh5AG147600 Rh5AG147700 Rh5BG147200 Rh5CG158600 Rh5DG146500
rosa_wichuraiana	Rw5G013050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1397
Acc36I	ACCTGC	2 cut(s)	28, 1753
AccI	GTMKAC	3 cut(s)	878, 964, 983
AccII	CGCG	3 cut(s)	108, 231, 1621
AciI	CCGC	6 cut(s)	231, 739, 804, 1031, 1267, 1314
AclWI	GGATC	1 cut(s)	319
AcoI	YGGCCR	1 cut(s)	1426
AcsI	RAATTY	5 cut(s)	236, 411, 1038, 1143, 1668
AcuI	CTGAAG	2 cut(s)	678, 738
AdeI	CACNNNGTG	1 cut(s)	761
AfaI	GTAC	7 cut(s)	378, 530, 651, 1088, 1504, 1725, 1771
AfiI	CCNNNNNNNGG	1 cut(s)	1709
AgsI	TTSAA	7 cut(s)	250, 544, 638, 941, 1256, 1594, 1743
AhdI	GACNNNNNGTC	1 cut(s)	1620
AjnI	CCWGG	1 cut(s)	1718
AjuI	GAANNNNNNNTTGG	2 cut(s)	711, 743
Alw21I	GWGCWC	1 cut(s)	539
Alw26I	GTCTC	2 cut(s)	485, 866
AlwI	GGATC	1 cut(s)	319
AlwNI	CAGNNNCTG	1 cut(s)	367
Ama87I	CYCGRG	1 cut(s)	1636
AoxI	GGCC	4 cut(s)	292, 684, 1426, 1435
ApeKI	GCWGC	5 cut(s)	141, 1136, 1159, 1247, 1490
ApoI	RAATTY	5 cut(s)	236, 411, 1038, 1143, 1668
ArsI	GACNNNNNNTTYG	2 cut(s)	1600, 1632
AspLEI	GCGC	2 cut(s)	110, 133
AspS9I	GGNCC	2 cut(s)	292, 1481
AsuC2I	CCSGG	3 cut(s)	296, 338, 1378
AsuHPI	GGTGA	1 cut(s)	352
AsuII	TTCGAA	1 cut(s)	1674
AvaI	CYCGRG	1 cut(s)	1636
AvaII	GGWCC	1 cut(s)	1481
BanII	GRGCYC	2 cut(s)	350, 539
BauI	CACGAG	1 cut(s)	1654
BbsI	GAAGAC	3 cut(s)	666, 743, 1220
Bbv12I	GWGCWC	1 cut(s)	539
BbvI	GCAGC	5 cut(s)	128, 1123, 1171, 1234, 1502
BccI	CCATC	7 cut(s)	36, 153, 294, 731, 871, 1444, 1549
BciT130I	CCWGG	1 cut(s)	1720
BcnI	CCSGG	3 cut(s)	296, 338, 1378
BcoDI	GTCTC	2 cut(s)	485, 866
BfaI	CTAG	2 cut(s)	267, 1388
BfmI	CTRYAG	1 cut(s)	1137
BfuAI	ACCTGC	2 cut(s)	28, 1753
BglI	GCCNNNNNGGC	1 cut(s)	1434
BglII	AGATCT	1 cut(s)	828
BisI	GCNGC	5 cut(s)	142, 1137, 1160, 1248, 1491
BlsI	GCNGC	5 cut(s)	143, 1138, 1161, 1249, 1492
BmcAI	AGTACT	1 cut(s)	1771
Bme1390I	CCNGG	4 cut(s)	296, 338, 1378, 1720
Bme18I	GGWCC	1 cut(s)	1481
BmeRI	GACNNNNNGTC	1 cut(s)	1620
BmeT110I	CYCGRG	1 cut(s)	1636
BmgT120I	GGNCC	2 cut(s)	292, 1481
BmiI	GGNNCC	3 cut(s)	293, 499, 1483
BmrFI	CCNGG	4 cut(s)	296, 338, 1378, 1720
BpiI	GAAGAC	3 cut(s)	666, 743, 1220
Bpu14I	TTCGAA	1 cut(s)	1674
BpuMI	CCSGG	3 cut(s)	296, 338, 1378
BsaAI	YACGTR	1 cut(s)	981
BsaBI	GATNNNNATC	1 cut(s)	887
BsaJI	CCNNGG	2 cut(s)	295, 1719
BsaWI	WCCGGW	1 cut(s)	1597
BsaXI	ACNNNNNCTCC	4 cut(s)	521, 551, 1210, 1240
Bsc4I	CCNNNNNNNGG	1 cut(s)	1709
Bse118I	RCCGGY	2 cut(s)	1428, 1691
Bse1I	ACTGG	4 cut(s)	60, 177, 358, 1438
Bse3DI	GCAATG	1 cut(s)	1476
Bse8I	GATNNNNATC	1 cut(s)	887
BseBI	CCWGG	1 cut(s)	1720
BseDI	CCNNGG	2 cut(s)	295, 1719
BseGI	GGATG	4 cut(s)	305, 846, 1175, 1606
BseJI	GATNNNNATC	1 cut(s)	887
BseLI	CCNNNNNNNGG	1 cut(s)	1709
BseMI	GCAATG	1 cut(s)	1476
BseMII	CTCAG	1 cut(s)	861
BseNI	ACTGG	4 cut(s)	60, 177, 358, 1438
BseRI	GAGGAG	3 cut(s)	55, 58, 61
BseXI	GCAGC	5 cut(s)	128, 1123, 1171, 1234, 1502
BsgI	GTGCAG	1 cut(s)	1137
Bsh1236I	CGCG	3 cut(s)	108, 231, 1621
BshFI	GGCC	4 cut(s)	294, 686, 1428, 1437
BsiHKAI	GWGCWC	1 cut(s)	539
BsiHKCI	CYCGRG	1 cut(s)	1636
BsiSI	CCGG	7 cut(s)	295, 338, 1377, 1429, 1598, 1692, 1710
BslFI	GGGAC	2 cut(s)	461, 1025
BslI	CCNNNNNNNGG	1 cut(s)	1709
BsmAI	GTCTC	2 cut(s)	485, 866
BsmBI	CGTCTC	2 cut(s)	485, 866
BsmFI	GGGAC	2 cut(s)	461, 1025
BsnI	GGCC	4 cut(s)	294, 686, 1428, 1437
BsoBI	CYCGRG	1 cut(s)	1636
Bsp119I	TTCGAA	1 cut(s)	1674
Bsp1286I	GDGCHC	2 cut(s)	350, 539
Bsp1407I	TGTACA	1 cut(s)	528
Bsp143I	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
BspACI	CCGC	6 cut(s)	231, 739, 804, 1031, 1267, 1314
BspANI	GGCC	4 cut(s)	294, 686, 1428, 1437
BspCNI	CTCAG	1 cut(s)	862
BspFNI	CGCG	3 cut(s)	108, 231, 1621
BspHI	TCATGA	1 cut(s)	1231
BspLI	GGNNCC	3 cut(s)	293, 499, 1483
BspMAI	CTGCAG	1 cut(s)	1141
BspMI	ACCTGC	2 cut(s)	28, 1753
BspPI	GGATC	1 cut(s)	319
BspQI	GCTCTTC	3 cut(s)	191, 678, 1554
BspT104I	TTCGAA	1 cut(s)	1674
BsrDI	GCAATG	1 cut(s)	1476
BsrFI	RCCGGY	2 cut(s)	1428, 1691
BsrGI	TGTACA	1 cut(s)	528
BsrI	ACTGG	4 cut(s)	60, 177, 358, 1438
BssAI	RCCGGY	2 cut(s)	1428, 1691
BssECI	CCNNGG	2 cut(s)	295, 1719
BssMI	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
BssNAI	GTATAC	2 cut(s)	879, 984
BssSI	CACGAG	1 cut(s)	1654
Bst1107I	GTATAC	2 cut(s)	879, 984
Bst2BI	CACGAG	1 cut(s)	1654
Bst2UI	CCWGG	1 cut(s)	1720
Bst4CI	ACNGT	7 cut(s)	264, 401, 492, 1112, 1288, 1774, 1807
Bst6I	CTCTTC	5 cut(s)	191, 678, 839, 1251, 1554
BstAPI	GCANNNNNTGC	1 cut(s)	1247
BstAUI	TGTACA	1 cut(s)	528
BstBAI	YACGTR	1 cut(s)	981
BstBI	TTCGAA	1 cut(s)	1674
BstC8I	GCNNGC	3 cut(s)	25, 860, 1330
BstDEI	CTNAG	2 cut(s)	287, 870
BstF5I	GGATG	4 cut(s)	305, 846, 1175, 1606
BstFNI	CGCG	3 cut(s)	108, 231, 1621
BstHHI	GCGC	2 cut(s)	110, 133
BstKTI	GATC	6 cut(s)	99, 314, 394, 831, 853, 1753
BstMAI	GTCTC	2 cut(s)	485, 866
BstMBI	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
BstMWI	GCNNNNNNNGC	6 cut(s)	107, 595, 1091, 1247, 1434, 1715
BstNI	CCWGG	1 cut(s)	1720
BstNSI	RCATGY	2 cut(s)	27, 1414
BstSCI	CCNGG	4 cut(s)	294, 336, 1376, 1718
BstSFI	CTRYAG	1 cut(s)	1137
BstUI	CGCG	3 cut(s)	108, 231, 1621
BstV1I	GCAGC	5 cut(s)	128, 1123, 1171, 1234, 1502
BstV2I	GAAGAC	3 cut(s)	666, 743, 1220
BstX2I	RGATCY	1 cut(s)	828
BstYI	RGATCY	1 cut(s)	828
BstZ17I	GTATAC	2 cut(s)	879, 984
BsuRI	GGCC	4 cut(s)	294, 686, 1428, 1437
BtsCI	GGATG	4 cut(s)	305, 846, 1175, 1606
BtsIMutI	CAGTG	3 cut(s)	351, 497, 1293
BveI	ACCTGC	2 cut(s)	28, 1753
Cac8I	GCNNGC	3 cut(s)	25, 860, 1330
CaiI	CAGNNNCTG	1 cut(s)	367
CciI	TCATGA	1 cut(s)	1231
CfoI	GCGC	2 cut(s)	110, 133
Cfr10I	RCCGGY	2 cut(s)	1428, 1691
Cfr13I	GGNCC	2 cut(s)	292, 1481
CseI	GACGC	2 cut(s)	587, 1610
Csp6I	GTAC	7 cut(s)	377, 529, 650, 1087, 1503, 1724, 1770
CviAII	CATG	7 cut(s)	24, 91, 1165, 1201, 1232, 1411, 1611
CviQI	GTAC	7 cut(s)	377, 529, 650, 1087, 1503, 1724, 1770
DdeI	CTNAG	2 cut(s)	287, 870
DpnI	GATC	6 cut(s)	98, 313, 393, 830, 852, 1752
DpnII	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
DraI	TTTAAA	1 cut(s)	681
DraIII	CACNNNGTG	1 cut(s)	761
DrdI	GACNNNNNNGTC	1 cut(s)	1397
DriI	GACNNNNNGTC	1 cut(s)	1620
DseDI	GACNNNNNNGTC	1 cut(s)	1397
EaeI	YGGCCR	1 cut(s)	1426
Eam1104I	CTCTTC	5 cut(s)	191, 678, 839, 1251, 1554
Eam1105I	GACNNNNNGTC	1 cut(s)	1620
EarI	CTCTTC	5 cut(s)	191, 678, 839, 1251, 1554
EciI	GGCGGA	2 cut(s)	819, 1303
Ecl136II	GAGCTC	1 cut(s)	537
Eco147I	AGGCCT	1 cut(s)	686
Eco24I	GRGCYC	2 cut(s)	350, 539
Eco32I	GATATC	2 cut(s)	454, 1498
Eco47I	GGWCC	1 cut(s)	1481
Eco53kI	GAGCTC	1 cut(s)	537
Eco57I	CTGAAG	2 cut(s)	678, 738
Eco88I	CYCGRG	1 cut(s)	1636
EcoICRI	GAGCTC	1 cut(s)	537
EcoO109I	RGGNCCY	1 cut(s)	1481
EcoRII	CCWGG	1 cut(s)	1718
EcoRV	GATATC	2 cut(s)	454, 1498
EcoT22I	ATGCAT	2 cut(s)	1003, 1213
EcoT38I	GRGCYC	2 cut(s)	350, 539
Esp3I	CGTCTC	2 cut(s)	485, 866
FaeI	CATG	7 cut(s)	27, 94, 1168, 1204, 1235, 1414, 1614
FalI	AAGNNNNNCTT	4 cut(s)	524, 556, 901, 933
FaqI	GGGAC	2 cut(s)	461, 1025
FatI	CATG	7 cut(s)	23, 90, 1164, 1200, 1231, 1410, 1610
FauNDI	CATATG	4 cut(s)	443, 974, 1213, 1299
FblI	GTMKAC	3 cut(s)	878, 964, 983
Fnu4HI	GCNGC	5 cut(s)	142, 1137, 1160, 1248, 1491
FokI	GGATG	4 cut(s)	312, 853, 1182, 1613
FriOI	GRGCYC	2 cut(s)	350, 539
Fsp4HI	GCNGC	5 cut(s)	142, 1137, 1160, 1248, 1491
FspBI	CTAG	2 cut(s)	267, 1388
GlaI	GCGC	2 cut(s)	109, 132
GluI	GCNGC	5 cut(s)	142, 1137, 1160, 1248, 1491
HaeIII	GGCC	4 cut(s)	294, 686, 1428, 1437
HapII	CCGG	7 cut(s)	295, 338, 1377, 1429, 1598, 1692, 1710
HgaI	GACGC	2 cut(s)	587, 1610
HhaI	GCGC	2 cut(s)	110, 133
Hin1II	CATG	7 cut(s)	27, 94, 1168, 1204, 1235, 1414, 1614
Hin6I	GCGC	2 cut(s)	108, 131
HinP1I	GCGC	2 cut(s)	108, 131
HindIII	AAGCTT	2 cut(s)	244, 1104
HinfI	GANTC	3 cut(s)	364, 614, 1373
HpaII	CCGG	7 cut(s)	295, 338, 1377, 1429, 1598, 1692, 1710
HphI	GGTGA	1 cut(s)	352
Hpy166II	GTNNAC	6 cut(s)	325, 879, 965, 984, 1175, 1360
Hpy188I	TCNGA	3 cut(s)	242, 837, 1820
Hpy8I	GTNNAC	6 cut(s)	325, 879, 965, 984, 1175, 1360
Hpy99I	CGWCG	3 cut(s)	212, 581, 1751
HpyAV	CCTTC	5 cut(s)	376, 434, 550, 697, 897
HpyCH4III	ACNGT	7 cut(s)	264, 401, 492, 1112, 1288, 1774, 1807
HpyCH4IV	ACGT	4 cut(s)	462, 875, 980, 1335
HpyF10VI	GCNNNNNNNGC	6 cut(s)	107, 595, 1091, 1247, 1434, 1715
HpyF3I	CTNAG	2 cut(s)	287, 870
HpySE526I	ACGT	4 cut(s)	462, 875, 980, 1335
Hsp92II	CATG	7 cut(s)	27, 94, 1168, 1204, 1235, 1414, 1614
HspAI	GCGC	2 cut(s)	108, 131
Kzo9I	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
LguI	GCTCTTC	3 cut(s)	191, 678, 1554
LmnI	GCTCC	6 cut(s)	68, 542, 1133, 1384, 1628, 1733
Lsp1109I	GCAGC	5 cut(s)	128, 1123, 1171, 1234, 1502
MaeI	CTAG	2 cut(s)	267, 1388
MaeII	ACGT	4 cut(s)	462, 875, 980, 1335
MaeIII	GTNAC	2 cut(s)	792, 1606
MalI	GATC	6 cut(s)	98, 313, 393, 830, 852, 1752
MboI	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
MflI	RGATCY	1 cut(s)	828
MhlI	GDGCHC	2 cut(s)	350, 539
MluCI	AATT	8 cut(s)	76, 236, 411, 565, 892, 1038, 1143, 1668
MlyI	GAGTC	1 cut(s)	1382
MmeI	TCCRAC	1 cut(s)	1362
MnlI	CCTC	9 cut(s)	76, 79, 82, 442, 822, 831, 1002, 1342, 1789
Mph1103I	ATGCAT	2 cut(s)	1003, 1213
MseI	TTAA	6 cut(s)	459, 680, 786, 1404, 1415, 1521
MslI	CAYNNNNRTG	2 cut(s)	606, 1236
MspA1I	CMGCKG	1 cut(s)	1493
MspI	CCGG	7 cut(s)	295, 338, 1377, 1429, 1598, 1692, 1710
MspR9I	CCNGG	4 cut(s)	296, 338, 1378, 1720
MvaI	CCWGG	1 cut(s)	1720
MvnI	CGCG	3 cut(s)	108, 231, 1621
MwoI	GCNNNNNNNGC	6 cut(s)	107, 595, 1091, 1247, 1434, 1715
NciI	CCSGG	3 cut(s)	296, 338, 1378
NdeI	CATATG	4 cut(s)	443, 974, 1213, 1299
NdeII	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
NlaIII	CATG	7 cut(s)	27, 94, 1168, 1204, 1235, 1414, 1614
NlaIV	GGNNCC	3 cut(s)	293, 499, 1483
NmuCI	GTSAC	1 cut(s)	1606
NsiI	ATGCAT	2 cut(s)	1003, 1213
NspI	RCATGY	2 cut(s)	27, 1414
NspV	TTCGAA	1 cut(s)	1674
PaeI	GCATGC	1 cut(s)	27
PaeR7I	CTCGAG	1 cut(s)	1636
PagI	TCATGA	1 cut(s)	1231
PceI	AGGCCT	1 cut(s)	686
PciSI	GCTCTTC	3 cut(s)	191, 678, 1554
PcsI	WCGNNNNNNNCGW	1 cut(s)	1755
PfeI	GAWTC	2 cut(s)	364, 614
PkrI	GCNGC	5 cut(s)	143, 1138, 1161, 1249, 1492
PleI	GAGTC	1 cut(s)	1381
PpsI	GAGTC	1 cut(s)	1381
Ppu21I	YACGTR	1 cut(s)	981
PpuMI	RGGWCCY	1 cut(s)	1481
Psp124BI	GAGCTC	1 cut(s)	539
Psp5II	RGGWCCY	1 cut(s)	1481
Psp6I	CCWGG	1 cut(s)	1718
PspGI	CCWGG	1 cut(s)	1718
PspN4I	GGNNCC	3 cut(s)	293, 499, 1483
PspPI	GGNCC	2 cut(s)	292, 1481
PspPPI	RGGWCCY	1 cut(s)	1481
PstI	CTGCAG	1 cut(s)	1141
PstNI	CAGNNNCTG	1 cut(s)	367
PsuI	RGATCY	1 cut(s)	828
PvuII	CAGCTG	1 cut(s)	1493
RsaI	GTAC	7 cut(s)	378, 530, 651, 1088, 1504, 1725, 1771
RsaNI	GTAC	7 cut(s)	377, 529, 650, 1087, 1503, 1724, 1770
RseI	CAYNNNNRTG	2 cut(s)	606, 1236
SacI	GAGCTC	1 cut(s)	539
SapI	GCTCTTC	3 cut(s)	191, 678, 1554
SaqAI	TTAA	6 cut(s)	459, 680, 786, 1404, 1415, 1521
SatI	GCNGC	5 cut(s)	142, 1137, 1160, 1248, 1491
Sau3AI	GATC	6 cut(s)	96, 311, 391, 828, 850, 1750
Sau96I	GGNCC	2 cut(s)	292, 1481
ScaI	AGTACT	1 cut(s)	1771
SchI	GAGTC	1 cut(s)	1382
ScrFI	CCNGG	4 cut(s)	296, 338, 1378, 1720
SduI	GDGCHC	2 cut(s)	350, 539
SfcI	CTRYAG	1 cut(s)	1137
SfiI	GGCCNNNNNGGCC	1 cut(s)	1434
Sfr274I	CTCGAG	1 cut(s)	1636
SfuI	TTCGAA	1 cut(s)	1674
SinI	GGWCC	1 cut(s)	1481
SlaI	CTCGAG	1 cut(s)	1636
SmiMI	CAYNNNNRTG	2 cut(s)	606, 1236
SmlI	CTYRAG	1 cut(s)	1636
SmoI	CTYRAG	1 cut(s)	1636
SphI	GCATGC	1 cut(s)	27
Sse9I	AATT	8 cut(s)	76, 236, 411, 565, 892, 1038, 1143, 1668
SseBI	AGGCCT	1 cut(s)	686
SsiI	CCGC	6 cut(s)	231, 739, 804, 1031, 1267, 1314
SspMI	CTAG	2 cut(s)	267, 1388
SstI	GAGCTC	1 cut(s)	539
StuI	AGGCCT	1 cut(s)	686
StyD4I	CCNGG	4 cut(s)	294, 336, 1376, 1718
TaaI	ACNGT	7 cut(s)	264, 401, 492, 1112, 1288, 1774, 1807
TaiI	ACGT	4 cut(s)	465, 878, 983, 1338
TaqI	TCGA	9 cut(s)	390, 576, 617, 643, 708, 774, 1548, 1637, 1674
TasI	AATT	8 cut(s)	76, 236, 411, 565, 892, 1038, 1143, 1668
TatI	WGTACW	3 cut(s)	528, 1502, 1769
TfiI	GAWTC	2 cut(s)	364, 614
Tru1I	TTAA	6 cut(s)	459, 680, 786, 1404, 1415, 1521
Tru9I	TTAA	6 cut(s)	459, 680, 786, 1404, 1415, 1521
TscAI	CASTG	3 cut(s)	358, 497, 1293
TseFI	GTSAC	1 cut(s)	1606
TseI	GCWGC	5 cut(s)	141, 1136, 1159, 1247, 1490
Tsp45I	GTSAC	1 cut(s)	1606
TspDTI	ATGAA	6 cut(s)	79, 857, 962, 1220, 1248, 1277
TspGWI	ACGGA	5 cut(s)	225, 342, 915, 1029, 1146
TspRI	CASTG	3 cut(s)	358, 497, 1293
VpaK11BI	GGWCC	1 cut(s)	1481
XapI	RAATTY	5 cut(s)	236, 411, 1038, 1143, 1668
XceI	RCATGY	2 cut(s)	27, 1414
XhoI	CTCGAG	1 cut(s)	1636
XmiI	GTMKAC	3 cut(s)	878, 964, 983
XspI	CTAG	2 cut(s)	267, 1388
ZrmI	AGTACT	1 cut(s)	1771
Zsp2I	ATGCAT	2 cut(s)	1003, 1213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.