pycom10g21810

Calcyclin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006557
Physical Location & Seq
Forward (+)
24378820 .. 24379391
572 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 315 bp
ATGAAGATATACATATTTATGGAGGGAGATGATCAGGAGAAGATAGAGACCGACTTCAAGCCTATGTCATTTGACATCAAATTCCATGATGTGAAAGGAAAGAATTACAGGTTCGGTTTGCCTATGCTGAACAAGGAGATCGATCCAGAGAAGTGTAAGGTGCTGGTTCAGCCTACAAGGGTTGTTATCAATTTGGCTAAAACTTCAGAGGATTACTGGTTGGACTTGAAATTTAAGGAGGACAAGCTGACGCCGAGTCTGGATAAAGAGAAAGATCCCATGGCAGGAATCATGGACTTGATGAAGAATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
CS Siah-Interact_N

Protein Analysis

105

Amino Acids

12.28

Weight (kDa)

5.43

Isoelectric Point (pI)

22.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 2 - 67 7e-10 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 137, 269
AcsI RAATTY 2 cut(s) 80, 230
AcuI CTGAAG 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 284
AgsI TTSAA 2 cut(s) 58, 229
AhdI GACNNNNNGTC 1 cut(s) 255
AluBI AGCT 1 cut(s) 247
AluI AGCT 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 2 cut(s) 137, 269
ApoI RAATTY 2 cut(s) 80, 230
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 41
Bsp143I GATC 4 cut(s) 31, 138, 142, 274
Bsp19I CCATGG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 141
BspPI GGATC 2 cut(s) 137, 269
BspTNI GGTCTC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 4 cut(s) 31, 138, 142, 274
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 279
BstACI GRCGYC 1 cut(s) 251
BstDSI CCRYGG 1 cut(s) 279
BstKTI GATC 4 cut(s) 34, 141, 145, 277
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 4 cut(s) 31, 138, 142, 274
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 279
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 1 cut(s) 259
CviAII CATG 3 cut(s) 86, 280, 292
CviJI RGCY 4 cut(s) 61, 172, 197, 247
CviKI_1 RGCY 4 cut(s) 61, 172, 197, 247
DpnI GATC 4 cut(s) 33, 140, 144, 276
DpnII GATC 4 cut(s) 31, 138, 142, 274
DriI GACNNNNNGTC 1 cut(s) 255
Eam1105I GACNNNNNGTC 1 cut(s) 255
Eco130I CCWWGG 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 3 cut(s) 89, 283, 295
FaiI YATR 9 cut(s) 10, 14, 20, 65, 87, 125, 281, 293, 311
FatI CATG 3 cut(s) 85, 279, 291
FbaI TGATCA 1 cut(s) 31
HgaI GACGC 1 cut(s) 259
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 3 cut(s) 89, 283, 295
HinfI GANTC 2 cut(s) 256, 288
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 3 cut(s) 35, 146, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 3 cut(s) 89, 283, 295
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 4 cut(s) 31, 138, 142, 274
LpnPI CCDG 7 cut(s) 20, 94, 149, 159, 202, 245, 270
MalI GATC 4 cut(s) 33, 140, 144, 276
MboI GATC 4 cut(s) 31, 138, 142, 274
MboII GAAGA 2 cut(s) 16, 52
MflI RGATCY 1 cut(s) 274
MluCI AATT 4 cut(s) 80, 103, 190, 230
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 3 cut(s) 16, 202, 232
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 169
NcoI CCATGG 1 cut(s) 279
NdeII GATC 4 cut(s) 31, 138, 142, 274
NlaIII CATG 3 cut(s) 89, 283, 295
NmeAIII GCCGAG 1 cut(s) 279
PfeI GAWTC 1 cut(s) 288
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
PsuI RGATCY 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 234
Sau3AI GATC 4 cut(s) 31, 138, 142, 274
SchI GAGTC 1 cut(s) 265
SetI ASST 3 cut(s) 113, 162, 249
SmiMI CAYNNNNRTG 1 cut(s) 17
Sse9I AATT 4 cut(s) 80, 103, 190, 230
StyI CCWWGG 1 cut(s) 279
TaqI TCGA 1 cut(s) 141
TaqII GACCGA 1 cut(s) 65
TasI AATT 4 cut(s) 80, 103, 190, 230
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TspDTI ATGAA 1 cut(s) 17
XapI RAATTY 2 cut(s) 80, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.