RLG00000032092

Calcyclin-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006557
Physical Location & Seq
Reverse (-)
10837857 .. 10839717
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032092

Sequence Viewer

Length: 663 bp
ATGGCAGCAGAAGAAGAAGTGGCGCTAGATTTGGAGGAGCTCCGCCATATCCAGAGCATTGCAAAACGACCCCGTATTCTCAATCTCATCTCTTCTGAAATCGGCAATTTGGAAAAGTTGTCAAAAGAGGCTGCTCCTAAACCTACCTTGCCAACTCCAACTCCAATTTCTACAGCAGTGAAGGTGCCTTCCACCCCAGCTCTGAATTATGTTACACTTGGGTCATTCAGCTGGGAGCAAGATACTGATAAAGTGAAGATATATATATTTTTAGATGGAGTTGATCAGGAGAAAATGGAGACTGTGATCAAGCCTACTTCCATTGATATCAAATTGCATGATGTCCAAGGAAAGAACTACAGATGTGCTGTACCTAAACTCCATAGCCAGATTGTTCCAGAGAAGTGTAAGGTGCTAGTTAAGCCTACAAGGGTCGTGATTACATTGATTAAAGCTTCAAAGGGGAACTGGCTGGATCTGCATTTCAAGGAGGACAAGCTAAAGCCGAATTTGGATAAAGAGAAGGATCCTATGGCAGGAATCATGGACTTGATGAAGAATATGTACAATGAAGGGGATGAAGAAATGAAGCGAACAATTGCCAAAGCATGGACCGATGCGAGATCTGGAAAAACAGCCGATCCACTGAAGGGCTTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
CS Siah-Interact_N

Protein Analysis

221

Amino Acids

24.72

Weight (kDa)

8.66

Isoelectric Point (pI)

28.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 75 - 151 1.2e-15 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 609
AciI CCGC 1 cut(s) 43
AclWI GGATC 4 cut(s) 483, 521, 534, 635
AcsI RAATTY 1 cut(s) 508
AfaI GTAC 2 cut(s) 372, 566
AfiI CCNNNNNNNGG 3 cut(s) 536, 609, 650
AgsI TTSAA 2 cut(s) 459, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 151, 183
AluBI AGCT 5 cut(s) 40, 200, 231, 455, 499
AluI AGCT 5 cut(s) 40, 200, 231, 455, 499
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 4 cut(s) 483, 521, 534, 635
ApeKI GCWGC 2 cut(s) 5, 131
ApoI RAATTY 1 cut(s) 508
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 1 cut(s) 612
AvaII GGWCC 1 cut(s) 612
BamHI GGATCC 1 cut(s) 526
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 42
BarI GAAGNNNNNNTAC 2 cut(s) 548, 580
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 17, 118
BccI CCATC 1 cut(s) 269
BclI TGATCA 2 cut(s) 283, 306
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 2 cut(s) 26, 416
BfmI CTRYAG 2 cut(s) 171, 358
BfoI RGCGCY 1 cut(s) 26
BglII AGATCT 1 cut(s) 623
BisI GCNGC 2 cut(s) 6, 132
BlsI GCNGC 2 cut(s) 7, 133
Bme18I GGWCC 1 cut(s) 612
BmgT120I GGNCC 1 cut(s) 612
BmiI GGNNCC 2 cut(s) 186, 528
BmsI GCATC 1 cut(s) 607
BsaJI CCNNGG 1 cut(s) 346
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 363, 393
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 609, 650
Bse1I ACTGG 1 cut(s) 473
Bse3DI GCAATG 1 cut(s) 57
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 3 cut(s) 536, 609, 650
BseMI GCAATG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 473
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 17, 118
BseYI CCCAGC 2 cut(s) 196, 231
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 42
BslI CCNNNNNNNGG 3 cut(s) 536, 609, 650
BsmAI GTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 42
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 6 cut(s) 283, 306, 475, 526, 623, 640
BspACI CCGC 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 186, 528
BspPI GGATC 4 cut(s) 483, 521, 534, 635
BspT107I GGYRCC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 6 cut(s) 283, 306, 475, 526, 623, 640
BssT1I CCWWGG 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 97
BstAUI TGTACA 1 cut(s) 564
BstENI CCTNNNNNAGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 6 cut(s) 286, 309, 478, 529, 626, 643
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 6 cut(s) 283, 306, 475, 526, 623, 640
BstMWI GCNNNNNNNGC 2 cut(s) 421, 478
BstSFI CTRYAG 2 cut(s) 171, 358
BstV1I GCAGC 2 cut(s) 17, 118
BstX2I RGATCY 3 cut(s) 475, 526, 623
BstYI RGATCY 3 cut(s) 475, 526, 623
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 183, 644
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 612
Csp6I GTAC 2 cut(s) 371, 565
CviAII CATG 3 cut(s) 338, 544, 609
CviQI GTAC 2 cut(s) 371, 565
DpnI GATC 6 cut(s) 285, 308, 477, 528, 625, 642
DpnII GATC 6 cut(s) 283, 306, 475, 526, 623, 640
DraI TTTAAA 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 42
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 612
Eco53kI GAGCTC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 3 cut(s) 341, 547, 612
FatI CATG 3 cut(s) 337, 543, 608
FbaI TGATCA 2 cut(s) 283, 306
Fnu4HI GCNGC 2 cut(s) 6, 132
FokI GGATG 1 cut(s) 590
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 6, 132
FspBI CTAG 2 cut(s) 26, 416
GlaI GCGC 1 cut(s) 24
GluI GCNGC 2 cut(s) 6, 132
GsaI CCCAGC 2 cut(s) 200, 235
HaeII RGCGCY 1 cut(s) 26
HhaI GCGC 1 cut(s) 25
Hin1II CATG 3 cut(s) 341, 547, 612
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 1 cut(s) 540
Hpy188I TCNGA 2 cut(s) 97, 204
Hpy188III TCNNGA 5 cut(s) 52, 287, 398, 436, 627
HpyAV CCTTC 5 cut(s) 175, 198, 517, 566, 643
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4V TGCA 3 cut(s) 62, 337, 481
HpyF10VI GCNNNNNNNGC 2 cut(s) 421, 478
Hsp92II CATG 3 cut(s) 341, 547, 612
HspAI GCGC 1 cut(s) 23
Ksp22I TGATCA 2 cut(s) 283, 306
Kzo9I GATC 6 cut(s) 283, 306, 475, 526, 623, 640
LmnI GCTCC 4 cut(s) 37, 45, 139, 235
Lsp1109I GCAGC 2 cut(s) 17, 118
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 26, 416
MaeIII GTNAC 1 cut(s) 211
MalI GATC 6 cut(s) 285, 308, 477, 528, 625, 642
MboI GATC 6 cut(s) 283, 306, 475, 526, 623, 640
MboII GAAGA 6 cut(s) 23, 26, 84, 268, 568, 593
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 3 cut(s) 475, 526, 623
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 6 cut(s) 106, 165, 205, 332, 508, 597
MmeI TCCRAC 1 cut(s) 182
MnlI CCTC 3 cut(s) 28, 121, 484
MseI TTAA 3 cut(s) 420, 450, 657
MspA1I CMGCKG 1 cut(s) 231
MunI CAATTG 1 cut(s) 597
MwoI GCNNNNNNNGC 2 cut(s) 421, 478
NdeII GATC 6 cut(s) 283, 306, 475, 526, 623, 640
NlaIII CATG 3 cut(s) 341, 547, 612
NlaIV GGNNCC 2 cut(s) 186, 528
PfeI GAWTC 1 cut(s) 540
PflMI CCANNNNNTGG 1 cut(s) 609
PkrI GCNGC 2 cut(s) 7, 133
Psp124BI GAGCTC 1 cut(s) 42
PspFI CCCAGC 2 cut(s) 196, 231
PspN4I GGNNCC 2 cut(s) 186, 528
PspPI GGNCC 1 cut(s) 612
PsuI RGATCY 3 cut(s) 475, 526, 623
PvuII CAGCTG 1 cut(s) 231
RsaI GTAC 2 cut(s) 372, 566
RsaNI GTAC 2 cut(s) 371, 565
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 3 cut(s) 420, 450, 657
SatI GCNGC 2 cut(s) 6, 132
Sau3AI GATC 6 cut(s) 283, 306, 475, 526, 623, 640
Sau96I GGNCC 1 cut(s) 612
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 2 cut(s) 171, 358
SinI GGWCC 1 cut(s) 612
Sse9I AATT 6 cut(s) 106, 165, 205, 332, 508, 597
SsiI CCGC 1 cut(s) 43
SspMI CTAG 2 cut(s) 26, 416
SstI GAGCTC 1 cut(s) 42
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 304
TaqII GACCGA 1 cut(s) 629
TasI AATT 6 cut(s) 106, 165, 205, 332, 508, 597
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 3 cut(s) 420, 450, 657
Tru9I TTAA 3 cut(s) 420, 450, 657
TscAI CASTG 2 cut(s) 183, 651
TseI GCWGC 2 cut(s) 5, 131
TspDTI ATGAA 4 cut(s) 569, 585, 594, 602
TspRI CASTG 2 cut(s) 183, 651
Van91I CCANNNNNTGG 1 cut(s) 609
VpaK11BI GGWCC 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 534
XapI RAATTY 1 cut(s) 508
XspI CTAG 2 cut(s) 26, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.