pycom10g23280
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005527
Physical Location & Seq
Forward (+)
25481328 .. 25483690
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1194 bp
ATGGATGAGCTAGAATTTGGTTATCAGGAAGCTGAGAAGAGTAACGAGATCAGCGAGGAACAAGTAGAAAACGGATCAGTGGGATCGTCCCAGAAATTCTCATCCTTCGATTTGAATGAAGAAGCAAGTAGTGACAATGAAGAAAGAGGCCAAGGGAATAAAGCTGCAGATAATGATATCTCTAGTGGAGGAAATGAAGGGCAGAGGGTTAAAGTAAGGCAATATGTGAGATCGAAAATGCCTCGTCTTCGGTGGACACCTCAACTCCATCTTTCTTTTGTGCACGCTGTTGAAAGACTGGGTGGCCAAGATAGAGCAACTCCCAAATTAGTTCTTCAGCTAATGAATGTGAGAGGACTTAGCATTTCTCATGTAAAGAGTCACTTGCAGATGTATAGAAGTAAGAAGCTTGATGGGAATGGCCAAGTATTATCTGAAAACCACGAATCCAAGCATGGAAGGGATTACATATCTTGCATGGTGCACCAAACAATCAACCAGCCTCGGCGACATGTATACTTTAGAATGGACAACGGTGGCATCGTTGTAGCAACAAATCCACACGACTGTCATAGCCAATCACAGCCAGATTTTAAAGCTAGCTCAAGGTCAAGTTCTCTACTAAACAAAGAAGTCGCAAAAGGCAACAACTCGAACAAATCTATAGATTCAATCTTGCAAACTGTACCAATAAACCCTAGCAAGTTTCCTGAGGAAAAGAGGTGGCCTCCATTTGAAACGACTAACAAACAACAATGGAGGGCTAAAAGAATACCTGCTGCCAAAATCAATTGGTCGGATAATCGTAATGGCTCCCAATCTGACCATCTAATGCATCATATGCACACCACACCAAGGTTGATAGGGGAATCTTACAATTTAAGGCCACCAAACTGGCACCTTGGAGAGAGCAGAAACGTAAGGCAATTTCCATCAAACGCACACAATTCTATCACCAATTCCAGTTGCTACAAAACCGAATTCAAGTCGCCATTTAGGTTTGAGTTGAATGCAGAAAAATTTTCGAAGTACAAAGAATGGATGCCACAACTGGAATTGGGTCTGGGCCAAAGAGTTGAGAATTATGAGGAAAAGAAGATTACTTCCAGACATCGTATGGTTAGTGAAGATCATTATGGAACTGGGCAAAGAGTTAAACAGGAAATTAGCACTAAGCTTGCTCTTTCTCTTTAA

Protein Analysis

398

Amino Acids

45.53

Weight (kDa)

9.27

Isoelectric Point (pI)

59.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 82 - 133 4.8e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 897
AccI GTMKAC 1 cut(s) 516
AclWI GGATC 2 cut(s) 82, 91
AcoI YGGCCR 2 cut(s) 304, 421
AcsI RAATTY 4 cut(s) 14, 95, 980, 1019
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 2 cut(s) 687, 1031
AfiI CCNNNNNNNGG 1 cut(s) 855
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 6 cut(s) 115, 293, 672, 737, 985, 1009
AluBI AGCT 8 cut(s) 10, 32, 164, 340, 409, 599, 603, 1177
AluI AGCT 8 cut(s) 10, 32, 164, 340, 409, 599, 603, 1177
Alw21I GWGCWC 2 cut(s) 285, 486
Alw44I GTGCAC 2 cut(s) 281, 482
AlwI GGATC 2 cut(s) 82, 91
AoxI GGCC 6 cut(s) 148, 304, 421, 725, 884, 1066
ApaLI GTGCAC 2 cut(s) 281, 482
ApeKI GCWGC 2 cut(s) 164, 779
ApoI RAATTY 4 cut(s) 14, 95, 980, 1019
AspS9I GGNCC 1 cut(s) 1066
AsuHPI GGTGA 1 cut(s) 946
AsuII TTCGAA 1 cut(s) 1025
AsuNHI GCTAGC 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 2 cut(s) 285, 486
BalI TGGCCA 2 cut(s) 306, 423
BanI GGYRCC 1 cut(s) 897
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 285, 486
BbvI GCAGC 2 cut(s) 151, 766
BccI CCATC 4 cut(s) 276, 407, 834, 940
BfaI CTAG 4 cut(s) 11, 183, 600, 699
BfmI CTRYAG 2 cut(s) 165, 663
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 2 cut(s) 165, 780
BlsI GCNGC 2 cut(s) 166, 781
BmgT120I GGNCC 1 cut(s) 1066
BmiI GGNNCC 2 cut(s) 814, 899
BmrI ACTGGG 2 cut(s) 308, 1152
BmsI GCATC 3 cut(s) 549, 844, 1032
BmtI GCTAGC 1 cut(s) 603
BmuI ACTGGG 2 cut(s) 308, 1152
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 712, 744
Bpu14I TTCGAA 1 cut(s) 1025
BpuEI CTTGAG 1 cut(s) 589
BsaJI CCNNGG 4 cut(s) 151, 503, 854, 901
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 855
Bse1I ACTGG 5 cut(s) 303, 899, 963, 1056, 1147
Bse21I CCTNAGG 1 cut(s) 711
BseDI CCNNGG 4 cut(s) 151, 503, 854, 901
BseGI GGATG 3 cut(s) 10, 101, 1047
BseLI CCNNNNNNNGG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 24, 702
BseNI ACTGG 5 cut(s) 303, 899, 963, 1056, 1147
BseSI GKGCMC 2 cut(s) 285, 486
BseXI GCAGC 2 cut(s) 151, 766
BshFI GGCC 6 cut(s) 150, 306, 423, 727, 886, 1068
BshNI GGYRCC 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 285, 486
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 855
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 1015
BsnI GGCC 6 cut(s) 150, 306, 423, 727, 886, 1068
Bsp119I TTCGAA 1 cut(s) 1025
Bsp1286I GDGCHC 2 cut(s) 285, 486
Bsp143I GATC 5 cut(s) 48, 74, 83, 230, 1129
BspANI GGCC 6 cut(s) 150, 306, 423, 727, 886, 1068
BspCNI CTCAG 2 cut(s) 25, 703
BspLI GGNNCC 2 cut(s) 814, 899
BspMAI CTGCAG 1 cut(s) 169
BspMI ACCTGC 1 cut(s) 784
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 2 cut(s) 82, 91
BspT104I TTCGAA 1 cut(s) 1025
BspT107I GGYRCC 1 cut(s) 897
BsrI ACTGG 5 cut(s) 303, 899, 963, 1056, 1147
BssECI CCNNGG 4 cut(s) 151, 503, 854, 901
BssMI GATC 5 cut(s) 48, 74, 83, 230, 1129
BssNAI GTATAC 1 cut(s) 517
BssT1I CCWWGG 3 cut(s) 151, 854, 901
Bst1107I GTATAC 1 cut(s) 517
Bst4CI ACNGT 3 cut(s) 536, 569, 685
Bst6I CTCTTC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 3 cut(s) 285, 601, 1179
BstDEI CTNAG 4 cut(s) 33, 359, 711, 1173
BstF5I GGATG 3 cut(s) 10, 101, 1047
BstKTI GATC 5 cut(s) 51, 77, 86, 233, 1132
BstMBI GATC 5 cut(s) 48, 74, 83, 230, 1129
BstMWI GCNNNNNNNGC 1 cut(s) 841
BstNSI RCATGY 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 165, 663
BstSLI GKGCMC 2 cut(s) 285, 486
BstV1I GCAGC 2 cut(s) 151, 766
BstV2I GAAGAC 1 cut(s) 239
BstXI CCANNNNNNTGG 1 cut(s) 894
BstZ17I GTATAC 1 cut(s) 517
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 6 cut(s) 150, 306, 423, 727, 886, 1068
BtsCI GGATG 3 cut(s) 10, 101, 1047
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 3 cut(s) 285, 601, 1179
Cfr13I GGNCC 1 cut(s) 1066
Csp6I GTAC 2 cut(s) 686, 1030
CviAII CATG 4 cut(s) 371, 455, 478, 512
CviQI GTAC 2 cut(s) 686, 1030
DdeI CTNAG 4 cut(s) 33, 359, 711, 1173
DpnI GATC 5 cut(s) 50, 76, 85, 232, 1131
DpnII GATC 5 cut(s) 48, 74, 83, 230, 1129
DraI TTTAAA 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 304, 421
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 3 cut(s) 151, 854, 901
Eco32I GATATC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 320
Eco81I CCTNAGG 1 cut(s) 711
EcoRI GAATTC 1 cut(s) 980
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 3 cut(s) 151, 854, 901
EcoT22I ATGCAT 1 cut(s) 837
ErhI CCWWGG 3 cut(s) 151, 854, 901
FaeI CATG 4 cut(s) 374, 458, 481, 515
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 73
FatI CATG 4 cut(s) 370, 454, 477, 511
FauNDI CATATG 1 cut(s) 840
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 165, 780
FokI GGATG 3 cut(s) 17, 88, 1054
Fsp4HI GCNGC 2 cut(s) 165, 780
FspBI CTAG 4 cut(s) 11, 183, 600, 699
GluI GCNGC 2 cut(s) 165, 780
HaeIII GGCC 6 cut(s) 150, 306, 423, 727, 886, 1068
Hin1II CATG 4 cut(s) 374, 458, 481, 515
HindIII AAGCTT 2 cut(s) 407, 1175
HinfI GANTC 4 cut(s) 379, 446, 668, 869
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 4 cut(s) 255, 283, 484, 517
Hpy188I TCNGA 3 cut(s) 436, 799, 823
Hpy188III TCNNGA 3 cut(s) 26, 710, 1107
Hpy8I GTNNAC 4 cut(s) 255, 283, 484, 517
HpyAV CCTTC 3 cut(s) 115, 191, 453
HpyCH4III ACNGT 3 cut(s) 536, 569, 685
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 9 cut(s) 167, 283, 388, 477, 484, 679, 835, 844, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 841
HpyF3I CTNAG 4 cut(s) 33, 359, 711, 1173
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 4 cut(s) 374, 458, 481, 515
Kzo9I GATC 5 cut(s) 48, 74, 83, 230, 1129
LmnI GCTCC 1 cut(s) 818
Lsp1109I GCAGC 2 cut(s) 151, 766
LweI GCATC 3 cut(s) 549, 844, 1032
MaeI CTAG 4 cut(s) 11, 183, 600, 699
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 3 cut(s) 41, 131, 380
MalI GATC 5 cut(s) 50, 76, 85, 232, 1131
MboI GATC 5 cut(s) 48, 74, 83, 230, 1129
MboII GAAGA 7 cut(s) 49, 131, 152, 239, 326, 1108, 1139
MfeI CAATTG 1 cut(s) 790
MhlI GDGCHC 2 cut(s) 285, 486
MlsI TGGCCA 2 cut(s) 306, 423
MluNI TGGCCA 2 cut(s) 306, 423
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 777
Mox20I TGGCCA 2 cut(s) 306, 423
Mph1103I ATGCAT 1 cut(s) 837
MscI TGGCCA 2 cut(s) 306, 423
MseI TTAA 5 cut(s) 210, 594, 881, 1155, 1192
Msp20I TGGCCA 2 cut(s) 306, 423
MunI CAATTG 1 cut(s) 790
Mva1269I GAATGC 1 cut(s) 1015
MwoI GCNNNNNNNGC 1 cut(s) 841
NdeI CATATG 1 cut(s) 840
NdeII GATC 5 cut(s) 48, 74, 83, 230, 1129
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 4 cut(s) 374, 458, 481, 515
NlaIV GGNNCC 2 cut(s) 814, 899
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 131, 380
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 1 cut(s) 515
NspV TTCGAA 1 cut(s) 1025
PciI ACATGT 1 cut(s) 511
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 540
PctI GAATGC 1 cut(s) 1015
PfeI GAWTC 3 cut(s) 446, 668, 869
PkrI GCNGC 2 cut(s) 166, 781
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
PscI ACATGT 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 814, 899
PspPI GGNCC 1 cut(s) 1066
PstI CTGCAG 1 cut(s) 169
RsaI GTAC 2 cut(s) 687, 1031
RsaNI GTAC 2 cut(s) 686, 1030
SaqAI TTAA 5 cut(s) 210, 594, 881, 1155, 1192
SatI GCNGC 2 cut(s) 165, 780
Sau3AI GATC 5 cut(s) 48, 74, 83, 230, 1129
Sau96I GGNCC 1 cut(s) 1066
SchI GAGTC 1 cut(s) 388
SduI GDGCHC 2 cut(s) 285, 486
SfaNI GCATC 3 cut(s) 549, 844, 1032
SfcI CTRYAG 2 cut(s) 165, 663
SfuI TTCGAA 1 cut(s) 1025
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
SspMI CTAG 4 cut(s) 11, 183, 600, 699
StyI CCWWGG 3 cut(s) 151, 854, 901
TaaI ACNGT 3 cut(s) 536, 569, 685
TaiI ACGT 1 cut(s) 921
TaqI TCGA 4 cut(s) 108, 233, 653, 1025
TatI WGTACW 1 cut(s) 1029
TfiI GAWTC 3 cut(s) 446, 668, 869
Tru1I TTAA 5 cut(s) 210, 594, 881, 1155, 1192
Tru9I TTAA 5 cut(s) 210, 594, 881, 1155, 1192
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 2 cut(s) 131, 380
TseI GCWGC 2 cut(s) 164, 779
Tsp45I GTSAC 2 cut(s) 131, 380
TspDTI ATGAA 4 cut(s) 132, 153, 210, 359
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 84
VneI GTGCAC 2 cut(s) 281, 482
XapI RAATTY 4 cut(s) 14, 95, 980, 1019
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 4 cut(s) 11, 183, 600, 699
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.