pycom10g23770

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008848
Physical Location & Seq
Reverse (-)
25791389 .. 25799578
8190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGATTCTTACCCTAATTTGGGGTTCTGTTGTTGCTTTTGGTAGCTCCGACCTCTCGGATTCTCAAACTTTACCAAATCCAGAAAATGAGAAACCCTTCAGTTTAACTGGTTCTGGTGTAAGAACTGATGTCGAAACCCGAGATACTGCAAGAATCATGATGCTCTCGTTGATCCCATATCTCATTCTTCAGCTGGCAAAAATCTTGACTTCAACCACAGCGGTTCGAGTTGTTATTTTAATTTCTCTACTGATTGCATTTGCATTCCTTGCCACTTACTTCATTTACGAGTTCTTTCAACCATGGATTCAGAGCAGAAGGCTTGAATATTTGCTGCGTAAGTACATACCGAAAAACATACTGCCGAGTCTAATGACAGTAGGCGGAAACCCTAATGTATCTATTATAAAAGGGCTGTTCCATAGAATAGACAAGAATCATAACACATACATATCAGCAGATGAACTTAGAGCATTGATCCTAGGAATACAAATAGAAGCAATAGGTCTGGATGATGATGATTATGCAGAAGAAGTGATGAAGCAATTTGATCTCTCTGGCGACGCTCAAATAGCTGAGACGGAGTTTGTGAATGGACTCTCAAAATGGATTAATCCAGATAAGGCCCCTGCCAACGGTAAAGATAATGAACATAGATCGCTTTTCAGAAGAAATAATTCCAAGAATGAGACATCTATGGAAGATCAGCAGACACCGCTGATCTCTAAGAAAAAGAAGACTAGGGGTGCTGATACATCGTGGATGAATCTCCTGAAGGCCGCTTTTCTAATTATTCTAGGAACTGCCATAACGGCTTTTCTTTCAATGCCCCTCATGGTAGGTCTCCAAGAGTTTTCCACTGCTGCAAGCATCCCTTCTTTCACGACCTCGTATGTTGTGATACCCTTGGCTACAAATTACAGAGTGGCGCTAATGTCTGTATCGTCTGCCAAAGAAAAGACGGAGAATGCCATATCTTTAACGCTTTCTGAGATTTACAACGCTGCATTCATGAACAATATTGTGGGGTTGGTCATATTTTTGGCTCTTGTTTACATACGCAATTTATCATGGGATGTGGCTGCTGAAGTTTTAGTTGTTTTAATTATTTCGATAGCGGTTGGTCTTTCTGCAAGCTTCAGCAGAAGGTTCCCATTGTGGACATGCATTCTAGCATACATCCTGTACCCCATATCACTGCTGCTGGTTTATGTTCTTACCATTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

414

Amino Acids

45.99

Weight (kDa)

6.36

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 136 - 199 9.9e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
AciI CCGC 5 cut(s) 221, 384, 716, 780, 1118
AclWI GGATC 2 cut(s) 166, 472
AcuI CTGAAG 5 cut(s) 82, 173, 794, 1107, 1123
AfaI GTAC 2 cut(s) 344, 1187
AfiI CCNNNNNNNGG 3 cut(s) 18, 19, 635
AgsI TTSAA 4 cut(s) 213, 299, 326, 825
AluBI AGCT 4 cut(s) 45, 193, 575, 1137
AluI AGCT 4 cut(s) 45, 193, 575, 1137
Alw26I GTCTC 3 cut(s) 572, 683, 848
AlwI GGATC 2 cut(s) 166, 472
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 624, 777
ApeKI GCWGC 5 cut(s) 334, 863, 1004, 1082, 1201
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 2 cut(s) 95, 676
AspA2I CCTAGG 1 cut(s) 481
AspLEI GCGC 1 cut(s) 931
AspS9I GGNCC 1 cut(s) 625
AvaI CYCGRG 1 cut(s) 138
AvrII CCTAGG 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 5 cut(s) 321, 850, 991, 1069, 1188
BccI CCATC 1 cut(s) 1227
BceAI ACGGC 1 cut(s) 828
BcoDI GTCTC 3 cut(s) 572, 683, 848
BfaI CTAG 4 cut(s) 482, 741, 797, 1172
BfoI RGCGCY 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 812
BisI GCNGC 6 cut(s) 335, 780, 864, 1005, 1083, 1202
BlnI CCTAGG 1 cut(s) 481
BlsI GCNGC 6 cut(s) 336, 781, 865, 1006, 1084, 1203
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 2 cut(s) 627, 1151
BmsI GCATC 2 cut(s) 150, 879
BpiI GAAGAC 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 3 cut(s) 302, 481, 906
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 19, 635
Bse1I ACTGG 1 cut(s) 112
BseDI CCNNGG 3 cut(s) 302, 481, 906
BseGI GGATG 6 cut(s) 517, 768, 870, 1081, 1179, 1238
BseLI CCNNNNNNNGG 3 cut(s) 18, 19, 635
BseMII CTCAG 2 cut(s) 567, 981
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 5 cut(s) 321, 850, 991, 1069, 1188
BshFI GGCC 2 cut(s) 626, 779
BsiHKCI CYCGRG 1 cut(s) 138
BslI CCNNNNNNNGG 3 cut(s) 18, 19, 635
BsmAI GTCTC 3 cut(s) 572, 683, 848
BsmBI CGTCTC 1 cut(s) 572
BsmI GAATGC 4 cut(s) 263, 973, 1007, 1167
BsnI GGCC 2 cut(s) 626, 779
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 138
Bsp143I GATC 6 cut(s) 171, 477, 550, 656, 703, 720
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 5 cut(s) 221, 384, 716, 780, 1118
BspANI GGCC 2 cut(s) 626, 779
BspCNI CTCAG 2 cut(s) 568, 982
BspHI TCATGA 2 cut(s) 156, 1011
BspLI GGNNCC 2 cut(s) 627, 1151
BspPI GGATC 2 cut(s) 166, 472
BspTNI GGTCTC 1 cut(s) 848
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 3 cut(s) 302, 481, 906
BssMI GATC 6 cut(s) 171, 477, 550, 656, 703, 720
BssT1I CCWWGG 3 cut(s) 302, 481, 906
Bst4CI ACNGT 2 cut(s) 379, 638
BstAPI GCANNNNNTGC 1 cut(s) 269
BstC8I GCNNGC 3 cut(s) 195, 868, 1135
BstDEI CTNAG 4 cut(s) 467, 576, 726, 990
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 6 cut(s) 517, 768, 870, 1081, 1179, 1238
BstH2I RGCGCY 1 cut(s) 932
BstHHI GCGC 1 cut(s) 931
BstKTI GATC 6 cut(s) 174, 480, 553, 659, 706, 723
BstMAI GTCTC 3 cut(s) 572, 683, 848
BstMBI GATC 6 cut(s) 171, 477, 550, 656, 703, 720
BstMWI GCNNNNNNNGC 4 cut(s) 269, 572, 715, 812
BstNSI RCATGY 1 cut(s) 1167
BstV1I GCAGC 5 cut(s) 321, 850, 991, 1069, 1188
BstV2I GAAGAC 1 cut(s) 743
BsuRI GGCC 2 cut(s) 626, 779
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 6 cut(s) 517, 768, 870, 1081, 1179, 1238
BtsI GCAGTG 2 cut(s) 858, 1196
BtsIMutI CAGTG 2 cut(s) 858, 1196
Cac8I GCNNGC 3 cut(s) 195, 868, 1135
CciI TCATGA 2 cut(s) 156, 1011
CfoI GCGC 1 cut(s) 931
Cfr13I GGNCC 1 cut(s) 625
CseI GACGC 1 cut(s) 572
Csp6I GTAC 2 cut(s) 343, 1186
CviAII CATG 6 cut(s) 157, 303, 835, 1012, 1071, 1164
CviQI GTAC 2 cut(s) 343, 1186
DdeI CTNAG 4 cut(s) 467, 576, 726, 990
DpnI GATC 6 cut(s) 173, 479, 552, 658, 705, 722
DpnII GATC 6 cut(s) 171, 477, 550, 656, 703, 720
EciI GGCGGA 1 cut(s) 399
Eco130I CCWWGG 3 cut(s) 302, 481, 906
Eco31I GGTCTC 1 cut(s) 848
Eco57I CTGAAG 5 cut(s) 82, 173, 794, 1107, 1123
Eco88I CYCGRG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 625
EcoT14I CCWWGG 3 cut(s) 302, 481, 906
EcoT22I ATGCAT 1 cut(s) 1169
ErhI CCWWGG 3 cut(s) 302, 481, 906
Esp3I CGTCTC 1 cut(s) 572
FaeI CATG 6 cut(s) 160, 306, 838, 1015, 1074, 1167
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FatI CATG 6 cut(s) 156, 302, 834, 1011, 1070, 1163
Fnu4HI GCNGC 6 cut(s) 335, 780, 864, 1005, 1083, 1202
FokI GGATG 5 cut(s) 524, 775, 857, 1088, 1166
Fsp4HI GCNGC 6 cut(s) 335, 780, 864, 1005, 1083, 1202
FspBI CTAG 4 cut(s) 482, 741, 797, 1172
GlaI GCGC 1 cut(s) 930
GluI GCNGC 6 cut(s) 335, 780, 864, 1005, 1083, 1202
HaeII RGCGCY 1 cut(s) 932
HaeIII GGCC 2 cut(s) 626, 779
HgaI GACGC 1 cut(s) 572
HhaI GCGC 1 cut(s) 931
Hin1II CATG 6 cut(s) 160, 306, 838, 1015, 1074, 1167
Hin6I GCGC 1 cut(s) 929
HinP1I GCGC 1 cut(s) 929
HindIII AAGCTT 1 cut(s) 1135
HinfI GANTC 8 cut(s) 4, 59, 153, 307, 367, 436, 597, 766
Hpy166II GTNNAC 2 cut(s) 1054, 1161
Hpy188I TCNGA 6 cut(s) 49, 58, 312, 668, 991, 1232
Hpy188III TCNNGA 8 cut(s) 80, 157, 205, 509, 617, 772, 883, 1012
Hpy8I GTNNAC 2 cut(s) 1054, 1161
Hpy99I CGWCG 1 cut(s) 566
HpyAV CCTTC 5 cut(s) 106, 312, 769, 885, 1140
HpyCH4III ACNGT 2 cut(s) 379, 638
HpyCH4V TGCA 8 cut(s) 149, 257, 263, 527, 866, 1007, 1133, 1167
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 572, 715, 812
HpyF3I CTNAG 4 cut(s) 467, 576, 726, 990
Hsp92II CATG 6 cut(s) 160, 306, 838, 1015, 1074, 1167
HspAI GCGC 1 cut(s) 929
Kzo9I GATC 6 cut(s) 171, 477, 550, 656, 703, 720
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 5 cut(s) 321, 850, 991, 1069, 1188
LweI GCATC 2 cut(s) 150, 879
MaeI CTAG 4 cut(s) 482, 741, 797, 1172
MalI GATC 6 cut(s) 173, 479, 552, 658, 705, 722
MboI GATC 6 cut(s) 171, 477, 550, 656, 703, 720
MboII GAAGA 5 cut(s) 179, 542, 681, 713, 748
MluCI AATT 8 cut(s) 15, 240, 545, 676, 789, 916, 1063, 1104
MlyI GAGTC 2 cut(s) 376, 591
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 3 cut(s) 62, 842, 898
Mph1103I ATGCAT 1 cut(s) 1169
MroXI GAANNNNTTC 2 cut(s) 95, 676
MseI TTAA 6 cut(s) 104, 239, 612, 980, 1103, 1240
MspA1I CMGCKG 3 cut(s) 193, 221, 718
Mva1269I GAATGC 4 cut(s) 263, 973, 1007, 1167
MwoI GCNNNNNNNGC 4 cut(s) 269, 572, 715, 812
NcoI CCATGG 1 cut(s) 302
NdeII GATC 6 cut(s) 171, 477, 550, 656, 703, 720
NlaIII CATG 6 cut(s) 160, 306, 838, 1015, 1074, 1167
NlaIV GGNNCC 2 cut(s) 627, 1151
NmeAIII GCCGAG 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 1169
NspI RCATGY 1 cut(s) 1167
PagI TCATGA 2 cut(s) 156, 1011
PctI GAATGC 4 cut(s) 263, 973, 1007, 1167
PdmI GAANNNNTTC 2 cut(s) 95, 676
PfeI GAWTC 6 cut(s) 4, 59, 153, 307, 436, 766
PkrI GCNGC 6 cut(s) 336, 781, 865, 1006, 1084, 1203
PleI GAGTC 2 cut(s) 375, 591
PpsI GAGTC 2 cut(s) 375, 591
PshBI ATTAAT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 407
PspN4I GGNNCC 2 cut(s) 627, 1151
PspPI GGNCC 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 2 cut(s) 344, 1187
RsaNI GTAC 2 cut(s) 343, 1186
SaqAI TTAA 6 cut(s) 104, 239, 612, 980, 1103, 1240
SatI GCNGC 6 cut(s) 335, 780, 864, 1005, 1083, 1202
Sau3AI GATC 6 cut(s) 171, 477, 550, 656, 703, 720
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 2 cut(s) 376, 591
SetI ASST 9 cut(s) 47, 54, 195, 508, 577, 844, 890, 1139, 1151
SfaNI GCATC 2 cut(s) 150, 879
Sse9I AATT 8 cut(s) 15, 240, 545, 676, 789, 916, 1063, 1104
SsiI CCGC 5 cut(s) 221, 384, 716, 780, 1118
SspI AATATT 2 cut(s) 329, 1021
SspMI CTAG 4 cut(s) 482, 741, 797, 1172
StyI CCWWGG 3 cut(s) 302, 481, 906
TaaI ACNGT 2 cut(s) 379, 638
TaqI TCGA 3 cut(s) 132, 226, 1112
TasI AATT 8 cut(s) 15, 240, 545, 676, 789, 916, 1063, 1104
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 6 cut(s) 4, 59, 153, 307, 436, 766
Tru1I TTAA 6 cut(s) 104, 239, 612, 980, 1103, 1240
Tru9I TTAA 6 cut(s) 104, 239, 612, 980, 1103, 1240
TscAI CASTG 2 cut(s) 865, 1203
TseI GCWGC 5 cut(s) 334, 863, 1004, 1082, 1201
TspDTI ATGAA 7 cut(s) 271, 477, 554, 663, 779, 1000, 1028
TspGWI ACGGA 2 cut(s) 596, 977
TspRI CASTG 2 cut(s) 865, 1203
VspI ATTAAT 1 cut(s) 612
XceI RCATGY 1 cut(s) 1167
XmaJI CCTAGG 1 cut(s) 481
XmnI GAANNNNTTC 2 cut(s) 95, 676
XspI CTAG 4 cut(s) 482, 741, 797, 1172
Zsp2I ATGCAT 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.