pycom10g23800

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008848
Physical Location & Seq
Reverse (-)
25813334 .. 25815473
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGACAGAAATTCACATTTTGTTCCCCAGTTATTGGAGTTACAGCAACTACAGAAACTGCGGAGGAAATGGCAGAAATGAGCATGAGCATGCTTGCAGGATCGATCTGAGCTTCATCATGATTCTTACCATAATTTGGGGTTCTGCTGTTGCTTTCGGTAGCTCCGACCTCTCGGAGTCTCAAACTTCATCAAATCCAGAAAATAAGAAACCCTTCAGTTTAACTGATTCTGGTGTAAGAACTGATATCGAAGCCCGAGATACTGCAAGAATCATGATGCTCTCGTTGATCCCATATCTCATTCTTCAGCTGGCAAAAATCTTGACTTCAACTACAGCGGTTCGAGTTGCGATCTTAATTTCTCTACTGATTGCATCTGCATTCCTTGCCACTTACTTCATTTACCAGGTAACACACTTCAAGAATATAAAATCTGCTCGGCAATACTTAGATGAAATTCGAAATGTTGTATTCGTGAACTTCTTAACTGATAGAAAAAAAATTATGCAGTTCTTTCAACCATGGATTCAACACAGAAGGCTTGAATATTTGATGCGTAAATACATACCAAAAAGCATACTGGCGAGTCTTATGAGAGTAGGCGGAAACCCTAATGTATCCAGTATAAAAGGGCTGTTCCATAGAATAGACAAGAATAATAACGCATACATCTCAGCAGATGAGCTTAAAGCATTGATCCTAGGAATACAAATAAACGAAATAGGTCTGGATGACGATGATTATGTAGCAGAAGTGATGAAGCAATTTGATCCCTCTGGCGACGGTCAAATAGATGAGACGGAGTTTGTGAATGGAATCTCAAAATGGATTAATCTAGCTAAGCCCCCTGCCAACGGTAAAGGTCATGAACATAGATCGCTTTTCAGAAGAAATAATTCCAAGAGTTTCATCCGGGTCATTAAACTGCACTTGCAGAATGAGAAATCTATAGAAGATCAGCTTACGCGATCGATCTCTAAGAAAAAGGAGACTAAGGGTGCTACTACATCGTGGATGAATCTCCTGAAGGCTGCTTTTCTAATTATTCTAGGAACTGCCATGACGGTTTTTCTTTCAACGCCCCTCATGGAAAGTCTCCAAAAGTTTTCCACTGCTGCAAGCATCCCTTCTTTCGCGGTCTCGTATGTTGTGATACCCTTGGCTACAAACTACAGACTGGCGCTAATGTCAGTAACGTCTGCCAAAGAGAAGACGGAGAATGCCATCTCTTTAACACTTTCTGAGATTTACAACGCCACGTTCATGAACAACATTGTGGGGTTGGTCATATTTTTGGCGCTCGTTTATATACGCAATTTATCATGGGACGTGGCTGCTGATGTTTTAGTTGTTTTACTTATTTCGACAGCAGTTGGTCTTTCTACCAGCTTCAGCAGGAAGTTCCCATTTTGGACAAGCATTCTAGCATACATTCTGTACCCTCTATCACTGCTGCTGGTTTATGTTCTTACCACTGTTATCGGATGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

498

Amino Acids

55.99

Weight (kDa)

9.36

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 208 - 273 1.8e-12 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 33, 135
AccII CGCG 2 cut(s) 967, 1136
AciI CCGC 4 cut(s) 60, 338, 603, 1136
AclWI GGATC 4 cut(s) 107, 283, 691, 764
AcsI RAATTY 2 cut(s) 9, 456
AcuI CTGAAG 4 cut(s) 199, 290, 1046, 1375
AfaI GTAC 1 cut(s) 1439
AfiI CCNNNNNNNGG 3 cut(s) 33, 135, 854
AgsI TTSAA 6 cut(s) 330, 421, 518, 530, 545, 1077
AjiI CACGTC 1 cut(s) 1330
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 7 cut(s) 111, 162, 310, 685, 839, 961, 1389
AluI AGCT 7 cut(s) 111, 162, 310, 685, 839, 961, 1389
Alw26I GTCTC 5 cut(s) 183, 791, 983, 1100, 1144
AlwI GGATC 4 cut(s) 107, 283, 691, 764
AlwNI CAGNNNCTG 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 255
ApeKI GCWGC 4 cut(s) 1031, 1115, 1334, 1453
ApoI RAATTY 2 cut(s) 9, 456
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 2 cut(s) 212, 895
AspA2I CCTAGG 1 cut(s) 700
AspLEI GCGC 2 cut(s) 1183, 1300
AsuC2I CCSGG 1 cut(s) 914
AsuII TTCGAA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 255
AvrII CCTAGG 1 cut(s) 700
BbsI GAAGAC 1 cut(s) 1217
BbvI GCAGC 4 cut(s) 1018, 1102, 1321, 1440
BccI CCATC 2 cut(s) 1232, 1479
BciT130I CCWGG 1 cut(s) 407
BciVI GTATCC 1 cut(s) 628
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 5 cut(s) 183, 791, 983, 1100, 1144
BfaI CTAG 4 cut(s) 701, 836, 1049, 1424
BfmI CTRYAG 4 cut(s) 49, 333, 948, 1171
BfoI RGCGCY 2 cut(s) 1184, 1301
BfuI GTATCC 1 cut(s) 628
BisI GCNGC 4 cut(s) 1032, 1116, 1335, 1454
BlnI CCTAGG 1 cut(s) 700
BlpI GCTNAGC 1 cut(s) 840
BlsI GCNGC 4 cut(s) 1033, 1117, 1336, 1455
Bme1390I CCNGG 2 cut(s) 407, 914
BmeT110I CYCGRG 1 cut(s) 255
BmgBI CACGTC 1 cut(s) 1330
BmrFI CCNGG 2 cut(s) 407, 914
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 4 cut(s) 267, 383, 543, 1131
BmuI ACTGGG 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 1217
Bpu1102I GCTNAGC 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 914
Bsa29I ATCGAT 2 cut(s) 102, 971
BsaI GGTCTC 1 cut(s) 1144
BsaJI CCNNGG 3 cut(s) 521, 700, 1158
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 135, 854
Bse1I ACTGG 4 cut(s) 27, 585, 621, 1182
BseBI CCWGG 1 cut(s) 407
BseCI ATCGAT 2 cut(s) 102, 971
BseDI CCNNGG 3 cut(s) 521, 700, 1158
BseGI GGATG 5 cut(s) 736, 909, 1020, 1122, 1490
BseLI CCNNNNNNNGG 3 cut(s) 33, 135, 854
BseMII CTCAG 3 cut(s) 98, 687, 1233
BseNI ACTGG 4 cut(s) 27, 585, 621, 1182
BseXI GCAGC 4 cut(s) 1018, 1102, 1321, 1440
BsgI GTGCAG 1 cut(s) 911
Bsh1236I CGCG 2 cut(s) 967, 1136
Bsh1285I CGRYCG 1 cut(s) 971
BshVI ATCGAT 2 cut(s) 102, 971
BsiEI CGRYCG 1 cut(s) 971
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 913
BslFI GGGAC 1 cut(s) 1340
BslI CCNNNNNNNGG 3 cut(s) 33, 135, 854
BsmAI GTCTC 5 cut(s) 183, 791, 983, 1100, 1144
BsmBI CGTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 1340
BsmI GAATGC 3 cut(s) 380, 1225, 1419
Bso31I GGTCTC 1 cut(s) 1144
BsoBI CYCGRG 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 460
Bsp1720I GCTNAGC 1 cut(s) 840
Bsp19I CCATGG 1 cut(s) 521
BspACI CCGC 4 cut(s) 60, 338, 603, 1136
BspCNI CTCAG 3 cut(s) 99, 686, 1234
BspDI ATCGAT 2 cut(s) 102, 971
BspFNI CGCG 2 cut(s) 967, 1136
BspHI TCATGA 4 cut(s) 117, 273, 865, 1263
BspPI GGATC 4 cut(s) 107, 283, 691, 764
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 1144
BsrI ACTGG 4 cut(s) 27, 585, 621, 1182
BssECI CCNNGG 3 cut(s) 521, 700, 1158
BssT1I CCWWGG 3 cut(s) 521, 700, 1158
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 4 cut(s) 785, 857, 1066, 1477
BstAPI GCANNNNNTGC 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 4 cut(s) 90, 94, 312, 1120
BstDEI CTNAG 7 cut(s) 107, 448, 673, 840, 978, 993, 1242
BstDSI CCRYGG 1 cut(s) 521
BstF5I GGATG 5 cut(s) 736, 909, 1020, 1122, 1490
BstFNI CGCG 2 cut(s) 967, 1136
BstH2I RGCGCY 2 cut(s) 1184, 1301
BstHHI GCGC 2 cut(s) 1183, 1300
BstMAI GTCTC 5 cut(s) 183, 791, 983, 1100, 1144
BstMCI CGRYCG 1 cut(s) 971
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 405, 912
BstSFI CTRYAG 4 cut(s) 49, 333, 948, 1171
BstUI CGCG 2 cut(s) 967, 1136
BstV1I GCAGC 4 cut(s) 1018, 1102, 1321, 1440
BstV2I GAAGAC 1 cut(s) 1217
Bsu15I ATCGAT 2 cut(s) 102, 971
BsuI GTATCC 1 cut(s) 628
BsuTUI ATCGAT 2 cut(s) 102, 971
BtgI CCRYGG 1 cut(s) 521
BtrI CACGTC 1 cut(s) 1330
BtsCI GGATG 5 cut(s) 736, 909, 1020, 1122, 1490
BtsI GCAGTG 2 cut(s) 1110, 1448
BtsIMutI CAGTG 3 cut(s) 1110, 1448, 1473
Cac8I GCNNGC 4 cut(s) 90, 94, 312, 1120
CaiI CAGNNNCTG 1 cut(s) 57
CciI TCATGA 4 cut(s) 117, 273, 865, 1263
CfoI GCGC 2 cut(s) 1183, 1300
ClaI ATCGAT 2 cut(s) 102, 971
CsiI ACCWGGT 1 cut(s) 405
Csp6I GTAC 1 cut(s) 1438
CviQI GTAC 1 cut(s) 1438
DdeI CTNAG 7 cut(s) 107, 448, 673, 840, 978, 993, 1242
EciI GGCGGA 1 cut(s) 618
Eco130I CCWWGG 3 cut(s) 521, 700, 1158
Eco31I GGTCTC 1 cut(s) 1144
Eco32I GATATC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 199, 290, 1046, 1375
Eco88I CYCGRG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 405
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 3 cut(s) 521, 700, 1158
ErhI CCWWGG 3 cut(s) 521, 700, 1158
Esp3I CGTCTC 1 cut(s) 791
FalI AAGNNNNNCTT 6 cut(s) 197, 229, 945, 977, 1111, 1143
FaqI GGGAC 1 cut(s) 1340
Fnu4HI GCNGC 4 cut(s) 1032, 1116, 1335, 1454
FokI GGATG 4 cut(s) 743, 896, 1027, 1109
Fsp4HI GCNGC 4 cut(s) 1032, 1116, 1335, 1454
FspBI CTAG 4 cut(s) 701, 836, 1049, 1424
GlaI GCGC 2 cut(s) 1182, 1299
GluI GCNGC 4 cut(s) 1032, 1116, 1335, 1454
HaeII RGCGCY 2 cut(s) 1184, 1301
HapII CCGG 1 cut(s) 913
HhaI GCGC 2 cut(s) 1183, 1300
Hin6I GCGC 2 cut(s) 1181, 1298
HinP1I GCGC 2 cut(s) 1181, 1298
HinfI GANTC 8 cut(s) 121, 176, 227, 270, 526, 586, 816, 1018
HpaII CCGG 1 cut(s) 913
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 6 cut(s) 108, 166, 175, 887, 1243, 1484
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 4 cut(s) 223, 531, 1021, 1137
HpyCH4III ACNGT 4 cut(s) 785, 857, 1066, 1477
HpyCH4IV ACGT 3 cut(s) 1196, 1259, 1329
HpyCH4V TGCA 8 cut(s) 96, 266, 374, 380, 508, 928, 934, 1118
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 7 cut(s) 107, 448, 673, 840, 978, 993, 1242
HpySE526I ACGT 3 cut(s) 1196, 1259, 1329
HspAI GCGC 2 cut(s) 1181, 1298
LmnI GCTCC 1 cut(s) 167
Lsp1109I GCAGC 4 cut(s) 1018, 1102, 1321, 1440
LweI GCATC 4 cut(s) 267, 383, 543, 1131
MabI ACCWGGT 1 cut(s) 405
MaeI CTAG 4 cut(s) 701, 836, 1049, 1424
MaeII ACGT 3 cut(s) 1196, 1259, 1329
MaeIII GTNAC 3 cut(s) 38, 409, 1192
MboII GAAGA 4 cut(s) 296, 900, 965, 1222
MluCI AATT 9 cut(s) 9, 132, 357, 456, 501, 764, 895, 1041, 1315
MlyI GAGTC 2 cut(s) 185, 595
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 5 cut(s) 56, 179, 784, 1094, 1452
MroXI GAANNNNTTC 2 cut(s) 212, 895
MseI TTAA 8 cut(s) 221, 356, 485, 687, 831, 921, 1232, 1492
MslI CAYNNNNRTG 2 cut(s) 87, 1262
MspA1I CMGCKG 2 cut(s) 310, 338
MspI CCGG 1 cut(s) 913
MspR9I CCNGG 2 cut(s) 407, 914
Mva1269I GAATGC 3 cut(s) 380, 1225, 1419
MvaI CCWGG 1 cut(s) 407
MvnI CGCG 2 cut(s) 967, 1136
MwoI GCNNNNNNNGC 1 cut(s) 386
NciI CCSGG 1 cut(s) 914
NcoI CCATGG 1 cut(s) 521
NmeAIII GCCGAG 1 cut(s) 418
NspI RCATGY 1 cut(s) 92
NspV TTCGAA 1 cut(s) 460
PaeI GCATGC 1 cut(s) 92
PagI TCATGA 4 cut(s) 117, 273, 865, 1263
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 3 cut(s) 380, 1225, 1419
PdmI GAANNNNTTC 2 cut(s) 212, 895
PfeI GAWTC 6 cut(s) 121, 227, 270, 526, 816, 1018
PflMI CCANNNNNTGG 2 cut(s) 33, 135
PkrI GCNGC 4 cut(s) 1033, 1117, 1336, 1455
Ple19I CGATCG 1 cut(s) 971
PleI GAGTC 2 cut(s) 184, 594
PpsI GAGTC 2 cut(s) 184, 594
PshBI ATTAAT 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 405
PspGI CCWGG 1 cut(s) 405
PstNI CAGNNNCTG 1 cut(s) 57
PvuI CGATCG 1 cut(s) 971
PvuII CAGCTG 1 cut(s) 310
RsaI GTAC 1 cut(s) 1439
RsaNI GTAC 1 cut(s) 1438
RseI CAYNNNNRTG 2 cut(s) 87, 1262
SaqAI TTAA 8 cut(s) 221, 356, 485, 687, 831, 921, 1232, 1492
SatI GCNGC 4 cut(s) 1032, 1116, 1335, 1454
SchI GAGTC 2 cut(s) 185, 595
ScrFI CCNGG 2 cut(s) 407, 914
SexAI ACCWGGT 1 cut(s) 405
SfaNI GCATC 4 cut(s) 267, 383, 543, 1131
SfcI CTRYAG 4 cut(s) 49, 333, 948, 1171
SfuI TTCGAA 1 cut(s) 460
SmiMI CAYNNNNRTG 2 cut(s) 87, 1262
SphI GCATGC 1 cut(s) 92
Sse9I AATT 9 cut(s) 9, 132, 357, 456, 501, 764, 895, 1041, 1315
SsiI CCGC 4 cut(s) 60, 338, 603, 1136
SspI AATATT 1 cut(s) 548
SspMI CTAG 4 cut(s) 701, 836, 1049, 1424
StyD4I CCNGG 2 cut(s) 405, 912
StyI CCWWGG 3 cut(s) 521, 700, 1158
TaaI ACNGT 4 cut(s) 785, 857, 1066, 1477
TaiI ACGT 3 cut(s) 1199, 1262, 1332
TaqI TCGA 6 cut(s) 102, 249, 343, 460, 971, 1364
TasI AATT 9 cut(s) 9, 132, 357, 456, 501, 764, 895, 1041, 1315
TfiI GAWTC 6 cut(s) 121, 227, 270, 526, 816, 1018
Tru1I TTAA 8 cut(s) 221, 356, 485, 687, 831, 921, 1232, 1492
Tru9I TTAA 8 cut(s) 221, 356, 485, 687, 831, 921, 1232, 1492
TscAI CASTG 3 cut(s) 1117, 1455, 1480
TseI GCWGC 4 cut(s) 1031, 1115, 1334, 1453
TspGWI ACGGA 2 cut(s) 815, 1229
TspRI CASTG 3 cut(s) 1117, 1455, 1480
Van91I CCANNNNNTGG 2 cut(s) 33, 135
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 2 cut(s) 9, 456
XceI RCATGY 1 cut(s) 92
XmaJI CCTAGG 1 cut(s) 700
XmnI GAANNNNTTC 2 cut(s) 212, 895
XspI CTAG 4 cut(s) 701, 836, 1049, 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.