pycom10g23830

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004487
Physical Location & Seq
Reverse (-)
25825956 .. 25832250
6295 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1668 bp
ATGGCTGACCCCTCCGACGAGGCCAATCACATCGAGAAGCTTTACGAGTTCGGCGAACGACTGAACGAGTCCAAGGACAAGTCCCAGAATGTTAAGGATTACCAGGGGATTATCAATGCGGCCAAGACCAGTGTCAAGGCCAAGCAGCTGGCTGCCCAGCTTATTCCGAGGTTCTTCAAGTTCTTCCCCGAACTTTCGCAGCCCGCCATCGATACGCACCTCGATTTGATTGAAGAAGAAGAGCTCGGGGTACGAGTACAAGCTATTCGGGGCCTTCCCTTGTTCTGCAAGGATACGCCTGAACATATTGCGAAAATTGTAGACATTCTTGTGCAACTCCTTGCATCTGAGGAATTTGTGGAGCGTGATGCTGTGCATAAAGCACTTATGGCTCTTTTGAGGCAAGATGTTAAAGCTTCTTTGACAGCATTATTCAAGCACATTGGGAGTGTTGATGAACCAAGCACAGATGAGTTCATTCGGGAGAAGGTTTTAAGCTTCATTAGAGATAAGGTATTTCCTATTAAAGCTGAACTTTTGAAGCCTCAGGAAGAGATGGAAAGGCATATTACTGATTTGGTAAAAAAGAGTCTGGAAGATGTGACTGGTGCCGAGTTTAGAATGTTTATGGACTTCTTAAAAAGTTTGAGCATATTTGGGGAGAAAGCGCCTACTGAACGCATGAAAGAACTTATTGGAATTATTGAAGGACAAGCTGATTTAGATGCCCAATTCAATGTTTCAGATGCTGATCATATTGATAGGTTGATATCCTGCCTATTCATGGCTCTGCCATTTGTTGTGAGGGGTGCGTCAAGCAGCAAATTCCTAAATTATCTGGACAAACACATCCTTCCTGTTTCGGACAAGCTTCCTGATGAAAGAAGGCTAGATTTGCTCAAAGCCCTCGCAGAAGTTTCACCTTTCACAGCACCACAAGATTCTCGTCAGATCCTTCCTTCTGTTGTTCAGCTGTTAAAGAAAAACATGCCTCGGAGAAAGACCGGGGATGAGTTTAACTTCACTTATGTAGAATGCTTATTGTACACGTTTCACCATCTTGCTCACAAGGTTCCTAATGCTACTAACAGTCTATGCGGTTACAAGATAGTGACTGGGCAACCATCAGATAGACTTGGTGAGGACTTTTCAGAGCAATATAAGGAGTTTACTGAGAGGTTGAGTTATGTCGAGGAGCTAACTAGGGCCACCATGAAGAAATTAACACAGGGAATGGCTGAAAAGAACAAAGCGATGGCAGCTGCAAAATCTGACGAAGCAAAGGATACCATTAAAACTGAAAAGCAGAATACTACAACTGGGTTGCGAACCTGCAATAACATATTGGCAATGACAAAGACTCTACATTCAAAAACACCCTCATTCATTGGAGACAAGAGCATCAACCTGTCTTGGAAAGAAGCAACAAAACCCGCTGTGCCCTCTAGTACACCTGTTGCAGGAGCAAAACGACCTGCTAATTTTTCCAATGGATCCGGAAATATGCCTTCAAAAAAGGGGCGTGGTGGCGGATTACAAAATCAGCTTGTTAATAGAGCATTTGAAGGTTTATCTCAAGGTGGAAGAAGCGGGGGAAGGAGCGGTGGTGGTGGAAGGAGCAGAGGCAGAGGGTGGGGTGGACGTGGAAGGGGAAGGGGTTACAGATAG

Protein Analysis

556

Amino Acids

61.85

Weight (kDa)

8.95

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 11 - 553 1.1e-245 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1340, 1483
AccB1I GGYRCC 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 1602
AccI GTMKAC 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 1496
AciI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
AclWI GGATC 3 cut(s) 946, 1488, 1501
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 2 cut(s) 353, 824
AfaI GTAC 4 cut(s) 252, 258, 1046, 1450
AfiI CCNNNNNNNGG 2 cut(s) 784, 1460
AflIII ACRYGT 1 cut(s) 1047
AgsI TTSAA 9 cut(s) 178, 233, 436, 541, 707, 736, 1371, 1512, 1565
AjiI CACGTC 1 cut(s) 1643
AjnI CCWGG 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 1386
AlwI GGATC 3 cut(s) 946, 1488, 1501
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 1496
AoxI GGCC 5 cut(s) 21, 120, 138, 271, 1206
ApeKI GCWGC 6 cut(s) 145, 152, 199, 819, 1259, 1262
ApoI RAATTY 2 cut(s) 353, 824
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 271, 1206
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 3 cut(s) 912, 1046, 1151
AvaI CYCGRG 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 1443
BamHI GGATCC 1 cut(s) 1493
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BccI CCATC 5 cut(s) 215, 550, 1065, 1132, 1249
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 2 cut(s) 286, 1279
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 1 cut(s) 1386
BfaI CTAG 3 cut(s) 890, 1203, 1446
BfoI RGCGCY 1 cut(s) 671
BfuAI ACCTGC 2 cut(s) 1340, 1483
BfuI GTATCC 2 cut(s) 286, 1279
BisI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
BlsI GCNGC 7 cut(s) 121, 147, 154, 201, 821, 1261, 1264
Bme1390I CCNGG 2 cut(s) 104, 1006
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 1643
BmgT120I GGNCC 2 cut(s) 271, 1206
BmiI GGNNCC 4 cut(s) 272, 610, 1074, 1495
BmrFI CCNGG 2 cut(s) 104, 1006
BmrI ACTGGG 2 cut(s) 1125, 1329
BmsI GCATC 5 cut(s) 353, 358, 715, 736, 1410
BmuI ACTGGG 2 cut(s) 1125, 1329
BoxI GACNNNNGTC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 1560
BpuMI CCSGG 1 cut(s) 1006
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 750
BsaJI CCNNGG 5 cut(s) 72, 103, 167, 992, 1005
BsaWI WCCGGW 1 cut(s) 1496
BsaXI ACNNNNNCTCC 2 cut(s) 1591, 1621
Bsc4I CCNNNNNNNGG 2 cut(s) 784, 1460
Bse1I ACTGG 4 cut(s) 129, 610, 1120, 1324
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 1356
Bse8I GATNNNNATC 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 1496
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 72, 103, 167, 992, 1005
BseGI GGATG 2 cut(s) 849, 1015
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 2 cut(s) 784, 1460
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 3 cut(s) 339, 560, 1164
BseNI ACTGG 4 cut(s) 129, 610, 1120, 1324
BseRI GAGGAG 1 cut(s) 1208
BseSI GKGCMC 1 cut(s) 1443
BseXI GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BseYI CCCAGC 1 cut(s) 156
BshFI GGCC 5 cut(s) 23, 122, 140, 273, 1208
BshNI GGYRCC 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 2 cut(s) 1005, 1497
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 2 cut(s) 784, 1460
BsmAI GTCTC 1 cut(s) 1386
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 1040
BsnI GGCC 5 cut(s) 23, 122, 140, 273, 1208
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 246, 1443
Bsp13I TCCGGA 1 cut(s) 1496
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 3 cut(s) 751, 951, 1493
BspACI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
BspANI GGCC 5 cut(s) 23, 122, 140, 273, 1208
BspCNI CTCAG 3 cut(s) 340, 559, 1165
BspDI ATCGAT 1 cut(s) 210
BspEI TCCGGA 1 cut(s) 1496
BspLI GGNNCC 4 cut(s) 272, 610, 1074, 1495
BspMI ACCTGC 2 cut(s) 1340, 1483
BspPI GGATC 3 cut(s) 946, 1488, 1501
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 1602
BsrDI GCAATG 1 cut(s) 1356
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 4 cut(s) 129, 610, 1120, 1324
BssECI CCNNGG 5 cut(s) 72, 103, 167, 992, 1005
BssMI GATC 3 cut(s) 751, 951, 1493
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 1091
Bst6I CTCTTC 2 cut(s) 234, 546
BstAUI TGTACA 1 cut(s) 1044
BstC8I GCNNGC 2 cut(s) 150, 204
BstDEI CTNAG 3 cut(s) 348, 546, 1173
BstENI CCTNNNNNAGG 1 cut(s) 1458
BstF5I GGATG 2 cut(s) 849, 1015
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 3 cut(s) 754, 954, 1496
BstMAI GTCTC 1 cut(s) 1386
BstMBI GATC 3 cut(s) 751, 951, 1493
BstMWI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 991
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 102, 1004
BstSLI GKGCMC 1 cut(s) 1443
BstV1I GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BstX2I RGATCY 2 cut(s) 951, 1493
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 951, 1493
Bsu15I ATCGAT 1 cut(s) 210
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 2 cut(s) 286, 1279
BsuRI GGCC 5 cut(s) 23, 122, 140, 273, 1208
BsuTUI ATCGAT 1 cut(s) 210
BtgZI GCGATG 1 cut(s) 1268
BtrI CACGTC 1 cut(s) 1643
BtsCI GGATG 2 cut(s) 849, 1015
BtsIMutI CAGTG 1 cut(s) 136
BveI ACCTGC 2 cut(s) 1340, 1483
Cac8I GCNNGC 2 cut(s) 150, 204
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 271, 1206
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 1 cut(s) 801
Csp6I GTAC 4 cut(s) 251, 257, 1045, 1449
CviAII CATG 4 cut(s) 682, 784, 988, 1213
CviQI GTAC 4 cut(s) 251, 257, 1045, 1449
DdeI CTNAG 3 cut(s) 348, 546, 1173
DpnI GATC 3 cut(s) 753, 953, 1495
DpnII GATC 3 cut(s) 751, 951, 1493
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 234, 546
EarI CTCTTC 2 cut(s) 234, 546
EciI GGCGGA 1 cut(s) 1545
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 1458
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 685, 787, 991, 1216
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 681, 783, 987, 1212
FauI CCCGC 3 cut(s) 211, 1441, 1583
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
FokI GGATG 2 cut(s) 836, 1022
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
FspBI CTAG 3 cut(s) 890, 1203, 1446
GlaI GCGC 1 cut(s) 669
GluI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
GsaI CCCAGC 1 cut(s) 160
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 5 cut(s) 23, 122, 140, 273, 1208
HapII CCGG 2 cut(s) 1005, 1497
HgaI GACGC 1 cut(s) 801
HhaI GCGC 1 cut(s) 670
Hin1II CATG 4 cut(s) 685, 787, 991, 1216
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HindIII AAGCTT 4 cut(s) 38, 414, 496, 869
HinfI GANTC 4 cut(s) 68, 589, 941, 1360
HpaII CCGG 2 cut(s) 1005, 1497
HphI GGTGA 3 cut(s) 912, 1046, 1151
Hpy166II GTNNAC 5 cut(s) 322, 1047, 1170, 1451, 1640
Hpy188III TCNNGA 7 cut(s) 34, 482, 548, 593, 839, 875, 1497
Hpy8I GTNNAC 5 cut(s) 322, 1047, 1170, 1451, 1640
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 2 cut(s) 1049, 1642
HpyCH4V TGCA 7 cut(s) 288, 334, 344, 376, 1265, 1335, 1460
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
HpyF3I CTNAG 3 cut(s) 348, 546, 1173
HpySE526I ACGT 2 cut(s) 1049, 1642
Hsp92II CATG 4 cut(s) 685, 787, 991, 1216
HspAI GCGC 1 cut(s) 668
Kpn2I TCCGGA 1 cut(s) 1496
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 3 cut(s) 751, 951, 1493
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 361, 1195, 1463, 1599, 1617
Lsp1109I GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
LweI GCATC 5 cut(s) 353, 358, 715, 736, 1410
MaeI CTAG 3 cut(s) 890, 1203, 1446
MaeII ACGT 2 cut(s) 1049, 1642
MaeIII GTNAC 4 cut(s) 601, 1100, 1111, 1658
MalI GATC 3 cut(s) 753, 953, 1495
MbiI CCGCTC 1 cut(s) 1602
MboI GATC 3 cut(s) 751, 951, 1493
MboII GAAGA 9 cut(s) 166, 175, 245, 248, 251, 563, 608, 1228, 1596
MflI RGATCY 2 cut(s) 951, 1493
MhlI GDGCHC 2 cut(s) 246, 1443
MluCI AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
MlyI GAGTC 3 cut(s) 77, 598, 1354
MmeI TCCRAC 1 cut(s) 39
MroI TCCGGA 1 cut(s) 1496
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 4 cut(s) 148, 973, 1262, 1436
MspI CCGG 2 cut(s) 1005, 1497
MspR9I CCNGG 2 cut(s) 104, 1006
Mva1269I GAATGC 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
NciI CCSGG 1 cut(s) 1006
NdeII GATC 3 cut(s) 751, 951, 1493
NlaIII CATG 4 cut(s) 685, 787, 991, 1216
NlaIV GGNNCC 4 cut(s) 272, 610, 1074, 1495
NmeAIII GCCGAG 1 cut(s) 637
NmuCI GTSAC 2 cut(s) 601, 1111
NspI RCATGY 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 1040
PfeI GAWTC 1 cut(s) 941
PkrI GCNGC 7 cut(s) 121, 147, 154, 201, 821, 1261, 1264
PleI GAGTC 3 cut(s) 76, 597, 1354
PpsI GAGTC 3 cut(s) 76, 597, 1354
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 4 cut(s) 272, 610, 1074, 1495
PspPI GGNCC 2 cut(s) 271, 1206
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 2 cut(s) 951, 1493
PvuII CAGCTG 3 cut(s) 148, 973, 1262
RsaI GTAC 4 cut(s) 252, 258, 1046, 1450
RsaNI GTAC 4 cut(s) 251, 257, 1045, 1449
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 234
SatI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
Sau3AI GATC 3 cut(s) 751, 951, 1493
Sau96I GGNCC 2 cut(s) 271, 1206
SchI GAGTC 3 cut(s) 77, 598, 1354
ScrFI CCNGG 2 cut(s) 104, 1006
SduI GDGCHC 2 cut(s) 246, 1443
SfaNI GCATC 5 cut(s) 353, 358, 715, 736, 1410
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 1575
SmoI CTYRAG 1 cut(s) 1575
Sse9I AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
SsiI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
SspMI CTAG 3 cut(s) 890, 1203, 1446
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 102, 1004
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 2 cut(s) 1052, 1645
TaqI TCGA 4 cut(s) 33, 210, 222, 1191
TasI AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
TatI WGTACW 3 cut(s) 256, 1044, 1448
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 941
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 601, 1111
TseI GCWGC 6 cut(s) 145, 152, 199, 819, 1259, 1262
Tsp45I GTSAC 2 cut(s) 601, 1111
TspDTI ATGAA 8 cut(s) 466, 471, 490, 698, 772, 894, 1229, 1375
TspRI CASTG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 1458
XapI RAATTY 2 cut(s) 353, 824
XceI RCATGY 1 cut(s) 991
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 890, 1203, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.