pycom10g24210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007821
Physical Location & Seq
Forward (+)
26090893 .. 26091828
936 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGCTCCTCCTCCTACCAAATTCTTCTCTCTCCTCTTCCTCCTCTCTCTCATCCTCCTCTCTCTCCAAGCTCAAGCCAGAGACAGCCAATTCTTTAGCAAAGTCGCCAGCTCCAACAACAACAATGTGAAGGAATCAGAGCTCCCCAAAACAGAGTCCCCAAAAACAGAGACTCCAAAAACAGAGTCCCCTGTTTTCAATCCAGAAAAAGAGCAAGAGCCAACTTTCGTTCGAGAGACTCAAAACGGATACGGCCTCTACGGCCATGAGTCCGGCCAGCTGCCTCCTGCCACCTCCACCACTGGGACGGAGAAGCAGCACACTACAAACTACCCCGAAAGGTACAACACCCACTACAATCCCAACAAAAACAACTATTACAATGGGAACAACTTTGAGGCCAACCCTAATGGCCTCAGTGACACGAGGTTCGCTCAAAGCAGCTACACCACCACCACTGCCACCCCTAACAACCACCAGAACAACAACAACTTTGAGGCCAACCCTAATGGCCTCAGTGACACAAGGTTCGCTCAGAGCAGCTACACCACCACCACCGCCACCCCTAACAACCACCAGAACAACAACTTTGAGGCCAACCCTAATGGCCTCAGCGACACGAGGTTCGGTCAGAGCAGCTACACCACCACCACCGCCACCCCGAACAACTACCAGAACAACAACTACTACAATTTTGAGAAGCAGGGGATGAGTGACACTAGGTTTTTGGAGAACGGCAAATATTACTACGACGCAAGAAGCGAGAGCAACTACAACCAGAACCAGTATGGAAACTCTAGGGTTGTGGACTCGAAAAACTGGTACAATAACAGGGGTAATAACGGCAACGGAAATGCGAATCAGAACACCTATAACACGATGGAAGAGTACCAGACCGAGGAGGAGCAACAGAGCGAAGATCAGTTCGTGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

312

Amino Acids

35.34

Weight (kDa)

5.05

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 558, 654
AcoI YGGCCR 2 cut(s) 262, 274
AcsI RAATTY 1 cut(s) 20
AfaI GTAC 3 cut(s) 344, 824, 890
AfiI CCNNNNNNNGG 2 cut(s) 303, 898
AgsI TTSAA 1 cut(s) 199
AluBI AGCT 7 cut(s) 71, 111, 142, 280, 444, 543, 639
AluI AGCT 7 cut(s) 71, 111, 142, 280, 444, 543, 639
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 3 cut(s) 75, 164, 230
AoxI GGCC 9 cut(s) 253, 262, 274, 399, 412, 498, 511, 594, 607
ApeKI GCWGC 5 cut(s) 280, 316, 441, 540, 636
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 8 cut(s) 170, 202, 413, 445, 512, 544, 608, 640
BanII GRGCYC 1 cut(s) 144
BauI CACGAG 2 cut(s) 424, 619
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 611
BbvI GCAGC 5 cut(s) 267, 328, 453, 552, 648
BccI CCATC 1 cut(s) 874
BceAI ACGGC 4 cut(s) 268, 277, 751, 859
BciVI GTATCC 1 cut(s) 242
BcoDI GTCTC 3 cut(s) 75, 164, 230
BfaI CTAG 2 cut(s) 720, 798
BfuI GTATCC 1 cut(s) 242
BglI GCCNNNNNGGC 1 cut(s) 261
BisI GCNGC 5 cut(s) 281, 317, 442, 541, 637
BlsI GCNGC 5 cut(s) 282, 318, 443, 542, 638
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BplI GAGNNNNNCTC 2 cut(s) 418, 450
Bpu10I CCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 897
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 303, 898
Bse1I ACTGG 3 cut(s) 307, 784, 824
BseDI CCNNGG 1 cut(s) 897
BseGI GGATG 2 cut(s) 51, 714
BseLI CCNNNNNNNGG 2 cut(s) 303, 898
BseMII CTCAG 4 cut(s) 430, 529, 548, 625
BseNI ACTGG 3 cut(s) 307, 784, 824
BseRI GAGGAG 5 cut(s) 23, 32, 47, 914, 917
BseXI GCAGC 5 cut(s) 267, 328, 453, 552, 648
BshFI GGCC 9 cut(s) 255, 264, 276, 401, 414, 500, 513, 596, 609
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 3 cut(s) 142, 172, 319
BslI CCNNNNNNNGG 2 cut(s) 303, 898
BsmAI GTCTC 3 cut(s) 75, 164, 230
BsmFI GGGAC 3 cut(s) 142, 172, 319
BsnI GGCC 9 cut(s) 255, 264, 276, 401, 414, 500, 513, 596, 609
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 1 cut(s) 919
BspACI CCGC 2 cut(s) 558, 654
BspANI GGCC 9 cut(s) 255, 264, 276, 401, 414, 500, 513, 596, 609
BspCNI CTCAG 4 cut(s) 429, 528, 547, 624
BspLI GGNNCC 1 cut(s) 6
BsrI ACTGG 3 cut(s) 307, 784, 824
BssECI CCNNGG 1 cut(s) 897
BssMI GATC 1 cut(s) 919
BssSI CACGAG 2 cut(s) 424, 619
Bst2BI CACGAG 2 cut(s) 424, 619
Bst6I CTCTTC 2 cut(s) 41, 879
BstC8I GCNNGC 2 cut(s) 109, 278
BstDEI CTNAG 4 cut(s) 416, 515, 534, 611
BstF5I GGATG 2 cut(s) 51, 714
BstKTI GATC 1 cut(s) 922
BstMAI GTCTC 3 cut(s) 75, 164, 230
BstMBI GATC 1 cut(s) 919
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstV1I GCAGC 5 cut(s) 267, 328, 453, 552, 648
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 9 cut(s) 255, 264, 276, 401, 414, 500, 513, 596, 609
BtsCI GGATG 2 cut(s) 51, 714
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 4 cut(s) 300, 424, 456, 523
Cac8I GCNNGC 2 cut(s) 109, 278
CseI GACGC 1 cut(s) 761
Csp6I GTAC 3 cut(s) 343, 823, 889
CviAII CATG 2 cut(s) 266, 933
CviQI GTAC 3 cut(s) 343, 823, 889
DdeI CTNAG 4 cut(s) 416, 515, 534, 611
DpnI GATC 1 cut(s) 921
DpnII GATC 1 cut(s) 919
EaeI YGGCCR 2 cut(s) 262, 274
Eam1104I CTCTTC 2 cut(s) 41, 879
EarI CTCTTC 2 cut(s) 41, 879
Ecl136II GAGCTC 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 144
FaeI CATG 2 cut(s) 269, 936
FaiI YATR 4 cut(s) 267, 789, 873, 934
FaqI GGGAC 3 cut(s) 142, 172, 319
FatI CATG 2 cut(s) 265, 932
Fnu4HI GCNGC 5 cut(s) 281, 317, 442, 541, 637
FokI GGATG 2 cut(s) 38, 721
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 5 cut(s) 281, 317, 442, 541, 637
FspBI CTAG 2 cut(s) 720, 798
GluI GCNGC 5 cut(s) 281, 317, 442, 541, 637
HaeIII GGCC 9 cut(s) 255, 264, 276, 401, 414, 500, 513, 596, 609
HapII CCGG 1 cut(s) 273
HgaI GACGC 1 cut(s) 761
Hin1II CATG 2 cut(s) 269, 936
HinfI GANTC 8 cut(s) 134, 155, 172, 185, 238, 269, 809, 859
HpaII CCGG 1 cut(s) 273
Hpy166II GTNNAC 1 cut(s) 808
Hpy188I TCNGA 4 cut(s) 139, 537, 633, 864
Hpy188III TCNNGA 2 cut(s) 203, 233
Hpy8I GTNNAC 1 cut(s) 808
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 1 cut(s) 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 4 cut(s) 416, 515, 534, 611
Hsp92II CATG 2 cut(s) 269, 936
Kzo9I GATC 1 cut(s) 919
LmnI GCTCC 4 cut(s) 10, 116, 147, 904
Lsp1109I GCAGC 5 cut(s) 267, 328, 453, 552, 648
MaeI CTAG 2 cut(s) 720, 798
MaeIII GTNAC 3 cut(s) 419, 518, 713
MalI GATC 1 cut(s) 921
MboI GATC 1 cut(s) 919
MboII GAAGA 4 cut(s) 16, 28, 896, 929
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 20, 89, 691
MlyI GAGTC 6 cut(s) 164, 166, 194, 232, 278, 803
MmeI TCCRAC 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 1 cut(s) 919
NlaIII CATG 2 cut(s) 269, 936
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 419, 518, 713
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PfeI GAWTC 2 cut(s) 134, 859
PkrI GCNGC 5 cut(s) 282, 318, 443, 542, 638
PleI GAGTC 6 cut(s) 163, 166, 193, 232, 277, 803
PpsI GAGTC 6 cut(s) 163, 166, 193, 232, 277, 803
Psp124BI GAGCTC 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 6
PsrI GAACNNNNNNTAC 2 cut(s) 668, 700
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 3 cut(s) 344, 824, 890
RsaNI GTAC 3 cut(s) 343, 823, 889
SacI GAGCTC 1 cut(s) 144
SatI GCNGC 5 cut(s) 281, 317, 442, 541, 637
Sau3AI GATC 1 cut(s) 919
SchI GAGTC 6 cut(s) 164, 166, 194, 232, 278, 803
SduI GDGCHC 1 cut(s) 144
SfiI GGCCNNNNNGGCC 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
Sse9I AATT 3 cut(s) 20, 89, 691
SsiI CCGC 2 cut(s) 558, 654
SspI AATATT 1 cut(s) 743
SspMI CTAG 2 cut(s) 720, 798
SstI GAGCTC 1 cut(s) 144
TaqI TCGA 2 cut(s) 232, 812
TaqII GACCGA 2 cut(s) 617, 911
TasI AATT 3 cut(s) 20, 89, 691
TfiI GAWTC 2 cut(s) 134, 859
TscAI CASTG 4 cut(s) 307, 424, 463, 523
TseFI GTSAC 3 cut(s) 419, 518, 713
TseI GCWGC 5 cut(s) 280, 316, 441, 540, 636
Tsp45I GTSAC 3 cut(s) 419, 518, 713
TspGWI ACGGA 3 cut(s) 261, 323, 864
TspRI CASTG 4 cut(s) 307, 424, 463, 523
XapI RAATTY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 785
XspI CTAG 2 cut(s) 720, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.