pycom10g27200

tRNA wybutosine-synthesizing protein 2 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006279
Physical Location & Seq
Reverse (-)
28239369 .. 28244079
4711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3246 bp
ATGCGAAAAAGAGAAACAAAAAATAATATTACCCAGACAGGCCTGACCGGACTTATTCCCAAACGACCCCTCCCTCCTTGCTCCCGAAACTGCTCTTCTGCTTCAGGAGCTGGGCCGAGACTTTTGGAGGGTTTTACAGAAGCTGCAAAAGAGATGGAGTTCGAGAAACGAAAGGCGGCGACGCTAGCGTCACTGCGGTCGGAGGAGACAGACAAATCACCGAAGGGCACGGTGGACGCCCCGATCATACCCCTCCTCGACGCCATCAACAGCCACCCCAATTATTTCACCACCAGCTCCTGCTCCGGCCGTATCTCTATCCTCTCCCAGCCCACGCACGTGACAAATCAGCCCAAGACCAAGAAGAAAGCTCTAGGTGGCACGTGGATCTTCATCTCCCACGACCCGGCGGATGCCGACTCTATCCTCGGCCTCCTCTTCCGCCCCGACCCGAATGACCAGAACGACGCACAGGACCAAAACGACCTCGTTTTTAGATTTGAGCCGCTTATTGTGGCGGTGGAGTGCAGGGACGTGGCGTCGGCGCAGTCGTTGGTTTCGAAGGCGATTGCTTGCGGGTTCAGAGAGTCCGGCATTACGAATTCGAACCGGCGCGTGATTATCGCGATTCGGTGCTCAATTCGGATGGAGGTGCCGCTGGGCAGCAGCCGGGAGGTGATGGTGTCGAGAGAGTACGTGAGGTTCCTTGTCGGGGTGGCGAACGAGAAAATGGAGGCCAACAGGAAGAGGACTGAGGCATTTTTCCAGGCTCTGCAGAGTGAGGGTGGAGGGTTTGTGGGTCCCACCGTTGCTAATGGCGAGATTGCGGCAGATGGGCTTTCGGATTCTGGGTCCAAGGAAGTTCCTGGTGGTTGTAGTCTGTCTGTTGTTGAGATGGAAATTGCCGGTGAACCCGAAGAGAATCTTTTCCTCTGGGGTCATTCGTCTTGTGCATTGGAAATCAAAAACCGGAATGGTGTTCTTGTGTTTGGTGGCTTTGGAGGCATGGGAAGACATGGGAGAAGAAGTAGTTCTTGGCTGGTTGATCCATTGTTTGGCACTTTGAGAGCAATTGATGCAGCCAGCACTCCGTCTCCACGGTTGGGTCACACTTCGTCTCTGGTTGGAGATTGTGTGTTTGTCATTGGAGGCAGGGCTGATCCTGAGAAAATTTTGAGTGATGTTTGGGTCCTCAATACGGCAAAGAATGAATGGAAGTTATTGGATTGTTCTGGGGACAAATTTCCTCCCAGGCATCGGCATGCTGCAGCTGTTGTAGGCTCAAAGATATATGTGTTTGGTGGACTTAACAACGATACCATCAGTTCTTCCTTACATGTTCTCGATACCGATAATCTGCAGTGGAAAGAGTTGATTGTCAGTGGCGAGCATCCATGTGCCAGACATTCTCACTCAATGGTGGCATATAGATCTCAACTTTATATTTTTGGAGGATATAGTGGTGAGAAAACACTTGCAGACTTGTATGTTTTTGATATCCAAACGTGTAAATGGAAGAATGAAAAGGCAGCTGGGAGAAGTCCACATGCAAGGTTCTCCCACTCAATGTTTGTCTACAAGAATTATCTTGGGGTTGTTGGTGGTTGTCCAGTTAAACAACGTTGCCAGGAGTTAGCACTACTTGATTTGAAGGTCCGTGTGTGGAGGCATGTGAAACTTGAATCCAATGGTGAAGATTTATTTGTGCGTGGCACAGCCAATGTTGTTGGCGACAATCTTATTATGATTGGTGGTGGGGCATCTTGTTATGCATTTGGGACAAAGTTTAGCAAGCCCATGAAAATCAACTTGCTTCCTCTTATGAGTTTAGATGATAATATTAAGCCAGATGACAGAGAAATGCATGCTCAACATTATGAAATGGTGAATAATGAGAAAGGTAGACAATTCCAGGATCCACGGGCTGAAGGTGTGCAGAGCTTAAGTGAACCTTGTGATCTGAATTTTGAGAGTGACTTGCCCATGGAAAATGGCATAAGTCAGCAGGTAGAATCATATTGGATTCTACAACTTGAAAGAAAATATGCAAAAGTAGGCAAGGACATATTGAAGAAGTTTGGATGGTTAGATCTTGGAAGGAAGGTGTACTCCAGGAAGAGTGGTTCACATATATGTTTTCCTGTGAACGAAAAATTTTCATGCGTGTTTAAAGAAAACATGCATTCTTTGACAGATTTATCTGAAGGACAGAGTAATCACTTTGTTAAACCAGTTATAGGAGAAGAATTCTCAGTTAATGAGGTGAACTGTTCAAAAGCCTTGGACATTCTAAAGGAATGTGGTGCTACTAAGCTGGCAGCTGAAGAAGTTGAAGTCAAGAGAGCTGCCAAATCTCCCGCGAAAGTAATGAATGAAATTGTGGGCTTGCTGATAAAGGAGAAGGGACTGCCTGCAGAACTCTTAGAGGAGCTACCTGCAAGATGGGAGCGACTTGGAGATATAGTTGTCCTTCCGGCGACATCGTTCAAGGATCCAATATGGGACTCACTAGGGATGGAGCTTTGGCATGTTATTTCCAAATCACTTAACGCTCATCGCCTTGCACGCCAAGGCCGAGTTGCGTCAACCGCTACAAGGGACAGTACATTGGAGATTCTTGTAGGAGATAATGGATGGGTTGAGCATCGCGAAAATGGCATTCTCTATTCTTTCGATGCTACTAAATGTATGTTCTCCTGGGGCAACCTATCTGAGAAGCTCCGCATGGCCTCAATGAATTGCAGAAATGAGATTATTGTGGATTTGTTTGCTGGAATTGGATATTTTGTTCTGCCATTTCTTGTCAGGGCCAACGCAAAACTAGTTTATGCTTGTGAATGGAATCCCCATGCTCTTGAGGCACTCCGGCGAAATGTGCAAGCCAATTCAGTTAGTGACCGATGCATTGTTCTTGAAGGAGATAACCGGATAGTGGCACCCAAAGGAGTGGCTGATCGGGTCTGTCTCGGTCTCATTCCAACAAGTGAAGGTAGCTGGGTCACTGCTGTTAGGGCATTAAGGAGCGAGGGCGGGATGCTACACGTGCATGGGAACGTCAAGGACTCAGAGGAGGATTCATGGGCTAAACATGTATCAGAATCAATAAGTGAAATTGCCAAATCTGATGGTCATTGTTGGGAAGTAAGAGTAGAACATGTCGAGAGGGTGAAATGGTACGCCCCACACATCCGTCATCTTGTTGCAGACGTTAGATGTCGACAGAGTATCCGAACATGGCCAAATTAG

Protein Analysis

1082

Amino Acids

119.67

Weight (kDa)

7.34

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW3 PF02676 56 - 259 3.2e-69 Methyltransferase TYW3
Kelch_KLHDC2_KLHL20_DRC7 PF24681 313 - 425 1.9e-16 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 325 - 427 5.1e-14 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 360 - 409 7e-06 Attractin/LZTR1 beta-propeller
Beta-prop_TYW4 PF13418 364 - 597 3.6e-26 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_2 PF07646 366 - 408 8e-07 Kelch motif
Kelch_1 PF01344 366 - 407 4.8e-08 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 369 - 585 2.8e-57 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
NANM PF24996 372 - 508 8.7e-07 N-acetylneuraminate epimerase
Beta-prop_Calicin PF13964 377 - 507 1.2e-08 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 393 - 458 1.3e-10 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 417 - 458 8.4e-10 Kelch motif
Kelch_2 PF07646 417 - 460 8.6e-09 Kelch motif
Beta-prop_FBX42 PF13415 418 - 587 1.1e-30 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 450 - 510 1.1e-11 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 452 - 536 1.3e-11 Host cell factor, Kelch-repeats domain
Kelch_FKB95 PF25210 464 - 585 1.1e-06 FKB95, Kelch-repeats domain
Kelch_1 PF01344 467 - 506 3.5e-08 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 480 - 592 4.4e-14 Attractin/LZTR1 beta-propeller
TYW2_N_2 PF25133 800 - 877 3.8e-15 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 881 - 1022 3.4e-34 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 187
Acc36I ACCTGC 2 cut(s) 1996, 2443
AccB1I GGYRCC 2 cut(s) 652, 2935
AccB7I CCANNNNNTGG 1 cut(s) 1055
AccI GTMKAC 3 cut(s) 1575, 1903, 3217
AccII CGCG 4 cut(s) 615, 626, 2360, 2649
AclI AACGTT 1 cut(s) 1621
AclWI GGATC 7 cut(s) 395, 1040, 1154, 1910, 1923, 2486, 2499
AcoI YGGCCR 2 cut(s) 307, 3236
AcsI RAATTY 6 cut(s) 601, 1170, 1241, 1963, 2153, 2246
AcuI CTGAAG 4 cut(s) 87, 1947, 2223, 2343
AcvI CACGTG 3 cut(s) 340, 384, 3043
AcyI GRCGYC 3 cut(s) 237, 261, 539
AfaI GTAC 4 cut(s) 695, 2108, 2605, 3176
AfiI CCNNNNNNNGG 9 cut(s) 406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
AflII CTTAAG 1 cut(s) 1942
AflIII ACRYGT 5 cut(s) 1336, 1505, 3040, 3088, 3154
AgsI TTSAA 8 cut(s) 1651, 1682, 2036, 2071, 2274, 2333, 2488, 2915
AhdI GACNNNNNGTC 1 cut(s) 538
AhlI ACTAGT 1 cut(s) 2821
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 7 cut(s) 765, 865, 1250, 1626, 1911, 2111, 2696
AjuI GAANNNNNNNTTGG 2 cut(s) 1018, 1050
AleI CACNNNNGTG 1 cut(s) 338
AloI GAACNNNNNNTCC 4 cut(s) 2107, 2139, 2772, 2804
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 6 cut(s) 112, 200, 1098, 1122, 2969, 2975
AlwI GGATC 7 cut(s) 395, 1040, 1154, 1910, 1923, 2486, 2499
AlwNI CAGNNNCTG 4 cut(s) 110, 143, 300, 772
AoxI GGCC 9 cut(s) 40, 113, 307, 430, 735, 2572, 2727, 2808, 3236
ApeKI GCWGC 9 cut(s) 143, 663, 666, 1079, 1265, 1268, 1529, 2318, 2345
ApoI RAATTY 6 cut(s) 601, 1170, 1241, 1963, 2153, 2246
Asp700I GAANNNNTTC 2 cut(s) 926, 1030
AspLEI GCGC 2 cut(s) 547, 615
AspS9I GGNCC 7 cut(s) 113, 475, 800, 852, 1189, 1654, 2808
AsuC2I CCSGG 2 cut(s) 407, 671
AsuHPI GGTGA 9 cut(s) 210, 280, 688, 920, 1475, 1703, 1897, 2275, 3178
AsuII TTCGAA 2 cut(s) 560, 605
AsuNHI GCTAGC 1 cut(s) 184
AvaII GGWCC 5 cut(s) 475, 800, 852, 1189, 1654
BaeGI GKGCMC 1 cut(s) 230
BalI TGGCCA 1 cut(s) 3238
BamHI GGATCC 2 cut(s) 1915, 2491
BanI GGYRCC 2 cut(s) 652, 2935
BbrPI CACGTG 3 cut(s) 340, 384, 3043
BbsI GAAGAC 1 cut(s) 1018
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 9 cut(s) 130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BceAI ACGGC 2 cut(s) 294, 1215
BciT130I CCWGG 7 cut(s) 767, 867, 1252, 1628, 1913, 2113, 2698
BciVI GTATCC 1 cut(s) 3236
BcnI CCSGG 2 cut(s) 407, 671
BcoDI GTCTC 6 cut(s) 112, 200, 1098, 1122, 2969, 2975
BcuI ACTAGT 1 cut(s) 2821
BfaI CTAG 4 cut(s) 185, 374, 2510, 2822
BfmI CTRYAG 4 cut(s) 773, 1266, 1358, 2412
BfrI CTTAAG 1 cut(s) 1942
BfuAI ACCTGC 2 cut(s) 1996, 2443
BfuI GTATCC 1 cut(s) 3236
BglII AGATCT 2 cut(s) 1430, 2089
Bme1390I CCNGG 9 cut(s) 407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
Bme18I GGWCC 5 cut(s) 475, 800, 852, 1189, 1654
BmeRI GACNNNNNGTC 1 cut(s) 538
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 7 cut(s) 113, 475, 800, 852, 1189, 1654, 2808
BmiI GGNNCC 9 cut(s) 654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
BmrFI CCNGG 9 cut(s) 407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
BmsI GCATC 9 cut(s) 403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
BmtI GCTAGC 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 1018
BpmI CTGGAG 1 cut(s) 2095
Bpu14I TTCGAA 2 cut(s) 560, 605
BpuEI CTTGAG 1 cut(s) 2876
BpuMI CCSGG 2 cut(s) 407, 671
BsaAI YACGTR 4 cut(s) 340, 384, 697, 3043
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 3 cut(s) 237, 261, 539
BsaI GGTCTC 1 cut(s) 2975
BsaJI CCNNGG 9 cut(s) 427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BsaWI WCCGGW 3 cut(s) 47, 969, 2925
Bsc4I CCNNNNNNNGG 9 cut(s) 406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
Bse118I RCCGGY 2 cut(s) 609, 905
Bse1I ACTGG 2 cut(s) 1610, 2231
Bse8I GATNNNNATC 1 cut(s) 392
BseBI CCWGG 7 cut(s) 767, 867, 1252, 1628, 1913, 2113, 2698
BseDI CCNNGG 9 cut(s) 427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BseGI GGATG 8 cut(s) 418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 9 cut(s) 406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
BseMII CTCAG 5 cut(s) 744, 1155, 2265, 2703, 3078
BseNI ACTGG 2 cut(s) 1610, 2231
BseRI GAGGAG 5 cut(s) 218, 245, 425, 2441, 3083
BseSI GKGCMC 1 cut(s) 230
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 9 cut(s) 130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BseYI CCCAGC 5 cut(s) 110, 327, 658, 1532, 2994
BsgI GTGCAG 2 cut(s) 547, 1955
Bsh1236I CGCG 4 cut(s) 615, 626, 2360, 2649
Bsh1285I CGRYCG 2 cut(s) 200, 310
BshFI GGCC 9 cut(s) 42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BshNI GGYRCC 2 cut(s) 652, 2935
BsiEI CGRYCG 2 cut(s) 200, 310
BsiHKAI GWGCWC 1 cut(s) 638
BslFI GGGAC 7 cut(s) 545, 786, 1250, 1792, 2418, 2516, 2612
BslI CCNNNNNNNGG 9 cut(s) 406, 712, 1055, 1103, 1198, 1257, 2237, 2595, 2932
BsmAI GTCTC 6 cut(s) 112, 200, 1098, 1122, 2969, 2975
BsmBI CGTCTC 2 cut(s) 1098, 1122
BsmFI GGGAC 7 cut(s) 545, 786, 1250, 1792, 2418, 2516, 2612
BsmI GAATGC 2 cut(s) 2182, 2658
BsnI GGCC 9 cut(s) 42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
Bso31I GGTCTC 1 cut(s) 2975
Bsp119I TTCGAA 2 cut(s) 560, 605
Bsp1286I GDGCHC 2 cut(s) 230, 638
Bsp19I CCATGG 1 cut(s) 1983
Bsp68I TCGCGA 2 cut(s) 626, 2649
BspANI GGCC 9 cut(s) 42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BspCNI CTCAG 5 cut(s) 745, 1156, 2264, 2704, 3077
BspFNI CGCG 4 cut(s) 615, 626, 2360, 2649
BspLI GGNNCC 9 cut(s) 654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
BspMAI CTGCAG 4 cut(s) 777, 1270, 1362, 2416
BspMI ACCTGC 2 cut(s) 1996, 2443
BspOI GCTAGC 1 cut(s) 188
BspPI GGATC 7 cut(s) 395, 1040, 1154, 1910, 1923, 2486, 2499
BspQI GCTCTTC 1 cut(s) 100
BspT104I TTCGAA 2 cut(s) 560, 605
BspT107I GGYRCC 2 cut(s) 652, 2935
BspTI CTTAAG 1 cut(s) 1942
BspTNI GGTCTC 1 cut(s) 2975
BsrFI RCCGGY 2 cut(s) 609, 905
BsrI ACTGG 2 cut(s) 1610, 2231
BssAI RCCGGY 2 cut(s) 609, 905
BssECI CCNNGG 9 cut(s) 427, 855, 1097, 1250, 1919, 1983, 2280, 2569, 2697
BssNI GRCGYC 3 cut(s) 237, 261, 539
BssT1I CCWWGG 4 cut(s) 855, 1983, 2280, 2569
Bst2UI CCWGG 7 cut(s) 767, 867, 1252, 1628, 1913, 2113, 2698
Bst4CI ACNGT 5 cut(s) 232, 808, 1101, 2270, 2603
Bst6I CTCTTC 5 cut(s) 100, 443, 740, 912, 2111
BstACI GRCGYC 3 cut(s) 237, 261, 539
BstAFI CTTAAG 1 cut(s) 1942
BstAPI GCANNNNNTGC 1 cut(s) 1076
BstBAI YACGTR 4 cut(s) 340, 384, 697, 3043
BstBI TTCGAA 2 cut(s) 560, 605
BstDEI CTNAG 7 cut(s) 753, 1164, 2251, 2310, 2422, 2712, 3064
BstDSI CCRYGG 3 cut(s) 1097, 1919, 1983
BstF5I GGATG 8 cut(s) 418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BstFNI CGCG 4 cut(s) 615, 626, 2360, 2649
BstHHI GCGC 2 cut(s) 547, 615
BstMAI GTCTC 6 cut(s) 112, 200, 1098, 1122, 2969, 2975
BstMCI CGRYCG 2 cut(s) 200, 310
BstNI CCWGG 7 cut(s) 767, 867, 1252, 1628, 1913, 2113, 2698
BstNSI RCATGY 9 cut(s) 1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
BstSCI CCNGG 9 cut(s) 405, 669, 765, 865, 1250, 1626, 1911, 2111, 2696
BstSFI CTRYAG 4 cut(s) 773, 1266, 1358, 2412
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 4 cut(s) 615, 626, 2360, 2649
BstV1I GCAGC 9 cut(s) 130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
BstV2I GAAGAC 1 cut(s) 1018
BstX2I RGATCY 5 cut(s) 387, 1430, 1915, 2089, 2491
BstXI CCANNNNNNTGG 1 cut(s) 2947
BstYI RGATCY 5 cut(s) 387, 1430, 1915, 2089, 2491
BstZI CGGCCG 1 cut(s) 307
BsuI GTATCC 1 cut(s) 3236
BsuRI GGCC 9 cut(s) 42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
BtgI CCRYGG 3 cut(s) 1097, 1919, 1983
BtgZI GCGATG 2 cut(s) 2540, 2630
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 8 cut(s) 418, 651, 1390, 2087, 2520, 2639, 3039, 3186
BtsI GCAGTG 3 cut(s) 191, 1367, 3000
BtsIMutI CAGTG 4 cut(s) 191, 1367, 1387, 3000
BtuMI TCGCGA 2 cut(s) 626, 2649
BveI ACCTGC 2 cut(s) 1996, 2443
CaiI CAGNNNCTG 4 cut(s) 110, 143, 300, 772
CfoI GCGC 2 cut(s) 547, 615
Cfr10I RCCGGY 2 cut(s) 609, 905
Cfr13I GGNCC 7 cut(s) 113, 475, 800, 852, 1189, 1654, 2808
CseI GACGC 7 cut(s) 177, 190, 245, 269, 476, 528, 2571
Csp6I GTAC 4 cut(s) 694, 2107, 2604, 3175
CviQI GTAC 4 cut(s) 694, 2107, 2604, 3175
DdeI CTNAG 7 cut(s) 753, 1164, 2251, 2310, 2422, 2712, 3064
DraI TTTAAA 1 cut(s) 2170
DrdI GACNNNNNNGTC 1 cut(s) 187
DriI GACNNNNNGTC 1 cut(s) 538
DseDI GACNNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 2 cut(s) 307, 3236
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 5 cut(s) 100, 443, 740, 912, 2111
Eam1105I GACNNNNNGTC 1 cut(s) 538
EarI CTCTTC 5 cut(s) 100, 443, 740, 912, 2111
EciI GGCGGA 2 cut(s) 425, 431
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 4 cut(s) 855, 1983, 2280, 2569
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 2975
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 5 cut(s) 475, 800, 852, 1189, 1654
Eco52I CGGCCG 1 cut(s) 307
Eco57I CTGAAG 4 cut(s) 87, 1947, 2223, 2343
Eco72I CACGTG 3 cut(s) 340, 384, 3043
EcoO109I RGGNCCY 2 cut(s) 800, 1189
EcoRI GAATTC 2 cut(s) 601, 2246
EcoRII CCWGG 7 cut(s) 765, 865, 1250, 1626, 1911, 2111, 2696
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 4 cut(s) 855, 1983, 2280, 2569
EcoT22I ATGCAT 4 cut(s) 1774, 1866, 2184, 2906
ErhI CCWWGG 4 cut(s) 855, 1983, 2280, 2569
Esp3I CGTCTC 2 cut(s) 1098, 1122
FalI AAGNNNNNCTT 6 cut(s) 909, 941, 1018, 1050, 1936, 1968
FaqI GGGAC 7 cut(s) 545, 786, 1250, 1792, 2418, 2516, 2612
FauI CCCGC 3 cut(s) 569, 2365, 3023
FblI GTMKAC 3 cut(s) 1575, 1903, 3217
FokI GGATG 8 cut(s) 425, 658, 1377, 2094, 2527, 2646, 3046, 3173
FspBI CTAG 4 cut(s) 185, 374, 2510, 2822
GlaI GCGC 2 cut(s) 546, 614
GsaI CCCAGC 5 cut(s) 114, 331, 662, 1536, 2998
GsuI CTGGAG 1 cut(s) 2095
HaeIII GGCC 9 cut(s) 42, 115, 309, 432, 737, 2574, 2729, 2810, 3238
HgaI GACGC 7 cut(s) 177, 190, 245, 269, 476, 528, 2571
HhaI GCGC 2 cut(s) 547, 615
Hin1I GRCGYC 3 cut(s) 237, 261, 539
Hin6I GCGC 2 cut(s) 545, 613
HinP1I GCGC 2 cut(s) 545, 613
HincII GTYRAC 2 cut(s) 2586, 3218
HindII GTYRAC 2 cut(s) 2586, 3218
HphI GGTGA 9 cut(s) 210, 280, 688, 920, 1475, 1703, 1897, 2275, 3178
Hpy99I CGWCG 4 cut(s) 184, 263, 470, 544
HpyCH4III ACNGT 5 cut(s) 232, 808, 1101, 2270, 2603
HpyCH4IV ACGT 9 cut(s) 339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
HpyF3I CTNAG 7 cut(s) 753, 1164, 2251, 2310, 2422, 2712, 3064
HpySE526I ACGT 9 cut(s) 339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
Hsp92I GRCGYC 3 cut(s) 237, 261, 539
HspAI GCGC 2 cut(s) 545, 613
KflI GGGWCCC 1 cut(s) 800
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 9 cut(s) 86, 107, 302, 308, 2428, 2446, 2518, 2724, 3021
Lsp1109I GCAGC 9 cut(s) 130, 675, 678, 1091, 1252, 1280, 1541, 2330, 2332
LweI GCATC 9 cut(s) 403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
MaeI CTAG 4 cut(s) 185, 374, 2510, 2822
MaeII ACGT 9 cut(s) 339, 383, 534, 696, 1505, 1621, 3042, 3054, 3207
MaeIII GTNAC 6 cut(s) 189, 340, 1106, 1973, 2894, 2998
MfeI CAATTG 1 cut(s) 1071
MflI RGATCY 5 cut(s) 387, 1430, 1915, 2089, 2491
MhlI GDGCHC 2 cut(s) 230, 638
MlsI TGGCCA 1 cut(s) 3238
MluNI TGGCCA 1 cut(s) 3238
MlyI GAGTC 4 cut(s) 413, 596, 2498, 3056
MmeI TCCRAC 3 cut(s) 180, 1105, 3002
Mox20I TGGCCA 1 cut(s) 3238
Mph1103I ATGCAT 4 cut(s) 1774, 1866, 2184, 2906
MroXI GAANNNNTTC 2 cut(s) 926, 1030
MscI TGGCCA 1 cut(s) 3238
MseI TTAA 9 cut(s) 1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
MslI CAYNNNNRTG 5 cut(s) 338, 1260, 1395, 2131, 3045
Msp20I TGGCCA 1 cut(s) 3238
MspA1I CMGCKG 4 cut(s) 658, 1271, 1532, 2321
MspCI CTTAAG 1 cut(s) 1942
MspR9I CCNGG 9 cut(s) 407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
MunI CAATTG 1 cut(s) 1071
Mva1269I GAATGC 2 cut(s) 2182, 2658
MvaI CCWGG 7 cut(s) 767, 867, 1252, 1628, 1913, 2113, 2698
MvnI CGCG 4 cut(s) 615, 626, 2360, 2649
NciI CCSGG 2 cut(s) 407, 671
NcoI CCATGG 1 cut(s) 1983
NheI GCTAGC 1 cut(s) 184
NlaIV GGNNCC 9 cut(s) 654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
NmeAIII GCCGAG 3 cut(s) 141, 408, 2600
NmuCI GTSAC 6 cut(s) 189, 340, 1106, 1973, 2894, 2998
NruI TCGCGA 2 cut(s) 626, 2649
NsiI ATGCAT 4 cut(s) 1774, 1866, 2184, 2906
NspI RCATGY 9 cut(s) 1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
NspV TTCGAA 2 cut(s) 560, 605
OliI CACNNNNGTG 1 cut(s) 338
PaeI GCATGC 2 cut(s) 1265, 1868
PceI AGGCCT 1 cut(s) 42
PciI ACATGT 3 cut(s) 1336, 3088, 3154
PciSI GCTCTTC 1 cut(s) 100
PcsI WCGNNNNNNNCGW 3 cut(s) 548, 557, 2572
PctI GAATGC 2 cut(s) 2182, 2658
PdmI GAANNNNTTC 2 cut(s) 926, 1030
PflMI CCANNNNNTGG 1 cut(s) 1055
PfoI TCCNGGA 2 cut(s) 1911, 2111
PleI GAGTC 4 cut(s) 413, 595, 2498, 3056
PmaCI CACGTG 3 cut(s) 340, 384, 3043
PmlI CACGTG 3 cut(s) 340, 384, 3043
PpsI GAGTC 4 cut(s) 413, 595, 2498, 3056
Ppu21I YACGTR 4 cut(s) 340, 384, 697, 3043
PpuMI RGGWCCY 2 cut(s) 800, 1189
PscI ACATGT 3 cut(s) 1336, 3088, 3154
Psp1406I AACGTT 1 cut(s) 1621
Psp5II RGGWCCY 2 cut(s) 800, 1189
Psp6I CCWGG 7 cut(s) 765, 865, 1250, 1626, 1911, 2111, 2696
PspCI CACGTG 3 cut(s) 340, 384, 3043
PspFI CCCAGC 5 cut(s) 110, 327, 658, 1532, 2994
PspGI CCWGG 7 cut(s) 765, 865, 1250, 1626, 1911, 2111, 2696
PspN4I GGNNCC 9 cut(s) 654, 704, 801, 802, 853, 1190, 1917, 2493, 2937
PspPI GGNCC 7 cut(s) 113, 475, 800, 852, 1189, 1654, 2808
PspPPI RGGWCCY 2 cut(s) 800, 1189
PsrI GAACNNNNNNTAC 2 cut(s) 686, 718
PstI CTGCAG 4 cut(s) 777, 1270, 1362, 2416
PstNI CAGNNNCTG 4 cut(s) 110, 143, 300, 772
PsuI RGATCY 5 cut(s) 387, 1430, 1915, 2089, 2491
PvuII CAGCTG 3 cut(s) 1271, 1532, 2321
RruI TCGCGA 2 cut(s) 626, 2649
RsaI GTAC 4 cut(s) 695, 2108, 2605, 3176
RsaNI GTAC 4 cut(s) 694, 2107, 2604, 3175
RseI CAYNNNNRTG 5 cut(s) 338, 1260, 1395, 2131, 3045
SalI GTCGAC 1 cut(s) 3216
SapI GCTCTTC 1 cut(s) 100
SaqAI TTAA 9 cut(s) 1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
Sau96I GGNCC 7 cut(s) 113, 475, 800, 852, 1189, 1654, 2808
SchI GAGTC 4 cut(s) 413, 596, 2498, 3056
ScrFI CCNGG 9 cut(s) 407, 671, 767, 867, 1252, 1628, 1913, 2113, 2698
SduI GDGCHC 2 cut(s) 230, 638
SfaNI GCATC 9 cut(s) 403, 1066, 1264, 1399, 1769, 2653, 2665, 2891, 3024
SfcI CTRYAG 4 cut(s) 773, 1266, 1358, 2412
SfuI TTCGAA 2 cut(s) 560, 605
SinI GGWCC 5 cut(s) 475, 800, 852, 1189, 1654
SmiMI CAYNNNNRTG 5 cut(s) 338, 1260, 1395, 2131, 3045
SmlI CTYRAG 2 cut(s) 1942, 2855
SmoI CTYRAG 2 cut(s) 1942, 2855
SpeI ACTAGT 1 cut(s) 2821
SphI GCATGC 2 cut(s) 1265, 1868
SseBI AGGCCT 1 cut(s) 42
SspI AATATT 2 cut(s) 28, 1840
SspMI CTAG 4 cut(s) 185, 374, 2510, 2822
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 9 cut(s) 405, 669, 765, 865, 1250, 1626, 1911, 2111, 2696
StyI CCWWGG 4 cut(s) 855, 1983, 2280, 2569
TaaI ACNGT 5 cut(s) 232, 808, 1101, 2270, 2603
TaiI ACGT 9 cut(s) 342, 386, 537, 699, 1508, 1624, 3045, 3057, 3210
TaqI TCGA 9 cut(s) 162, 258, 560, 605, 686, 1344, 2673, 3159, 3217
TaqII GACCGA 2 cut(s) 2913, 2957
TatI WGTACW 2 cut(s) 2106, 2603
TauI GCSGC 4 cut(s) 179, 508, 658, 830
Tru1I TTAA 9 cut(s) 1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
Tru9I TTAA 9 cut(s) 1308, 1614, 1842, 1943, 2169, 2226, 2256, 2547, 3017
TscAI CASTG 4 cut(s) 198, 1367, 1387, 3007
TseFI GTSAC 6 cut(s) 189, 340, 1106, 1973, 2894, 2998
TseI GCWGC 9 cut(s) 143, 663, 666, 1079, 1265, 1268, 1529, 2318, 2345
Tsp45I GTSAC 6 cut(s) 189, 340, 1106, 1973, 2894, 2998
TspGWI ACGGA 3 cut(s) 1080, 1646, 3179
TspRI CASTG 4 cut(s) 198, 1367, 1387, 3007
Van91I CCANNNNNTGG 1 cut(s) 1055
Vha464I CTTAAG 1 cut(s) 1942
VpaK11BI GGWCC 5 cut(s) 475, 800, 852, 1189, 1654
XapI RAATTY 6 cut(s) 601, 1170, 1241, 1963, 2153, 2246
XceI RCATGY 9 cut(s) 1265, 1340, 1550, 1673, 1868, 2182, 2531, 3092, 3158
XmiI GTMKAC 3 cut(s) 1575, 1903, 3217
XmnI GAANNNNTTC 2 cut(s) 926, 1030
XspI CTAG 4 cut(s) 185, 374, 2510, 2822
Zsp2I ATGCAT 4 cut(s) 1774, 1866, 2184, 2906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.