pycom11g01440

X-Pro dipeptidyl-peptidase (S15 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009940
Physical Location & Seq
Forward (+)
1234101 .. 1236106
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGCATCCCTATCCCAATCCCATGTCTAGTTCACCTCCTCCTCCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCATCCTGGATGTCAAACTGTACGGTGGAGTCCTGCACAGTTGAGACCAGCGATGGAGTCAAGCTCAGCACTCGGCTCTTCAAACCCAGAGAAGAAGAAGACATCAAGGAGGGCAGCCCTGTGGTGGTTCTGGTGCACCCATACTCCGTCCTTGGCGGCTGCCAAGGCCTGTTGAGAGGAATAGCAGCTGGGTTGGCAGACAGTGGTTATAAAGCTTTGACCTTTGACATGAGAGGGGTTGGGAGGTCAACAGGGAGGGCTTCTCTCACTGGGTTTGCAGAAATTAAGGATGTGATTTCTGTGTGCAAATGGGTTTGTGAGAATCTATCTGCTGACAGGATTATGTTGGTGGGTTCTTCTGCAGGTGCTCCAATTGCAGGCTCCGCAGTAGCTCAGATAGAACAAGTCGTGGGCTATGTTAGTCTGGGGTATCCTTTCGGCATGCTTGCCTCAATCCTTTTCGGGAGACACCACAAAGCCGTACTACAATCCTCAAAACCCAAACTCTTCGTAATGGGGACTCAGGATGGGTTCACGAGCGTGAAGCAGTTACAGAACAAGCTAAGTTCTGCAGCCGGTCGTGTTGAAACGCACCTGATTGAAGGAGCAAGCCACTTCCAAATGGAAGGCCCTGCTTATGATGCACAGATGGTGAATCTTATCCTTGAATTTATTGCTTCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4 Peptidase_S15

Protein Analysis

255

Amino Acids

26.97

Weight (kDa)

6.59

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S15 PF02129 44 - 152 3.5e-11 X-Pro dipeptidyl-peptidase (S15 family)
Abhydrolase_1 PF00561 80 - 161 4.7e-09 alpha/beta hydrolase fold
Hydrolase_4 PF12146 82 - 166 1.9e-07 Serine aminopeptidase, S33
Abhydrolase_11 PF20408 89 - 231 3.2e-09 Alpha/beta hydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AarI CACCTGC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 431
AciI CCGC 2 cut(s) 234, 462
AcsI RAATTY 1 cut(s) 746
AfaI GTAC 2 cut(s) 100, 561
AfiI CCNNNNNNNGG 3 cut(s) 84, 202, 455
AgsI TTSAA 4 cut(s) 160, 665, 680, 746
AjnI CCWGG 1 cut(s) 83
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 5 cut(s) 142, 266, 293, 470, 640
AluI AGCT 5 cut(s) 142, 266, 293, 470, 640
Alw21I GWGCWC 2 cut(s) 216, 448
Alw26I GTCTC 2 cut(s) 116, 538
Alw44I GTGCAC 1 cut(s) 212
AoxI GGCC 2 cut(s) 244, 706
ApaLI GTGCAC 1 cut(s) 212
ApeKI GCWGC 4 cut(s) 192, 237, 263, 650
ApoI RAATTY 1 cut(s) 746
AspS9I GGNCC 1 cut(s) 707
AsuHPI GGTGA 2 cut(s) 24, 742
BaeGI GKGCMC 1 cut(s) 216
BauI CACGAG 1 cut(s) 613
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 216, 448
BbvI GCAGC 4 cut(s) 204, 224, 275, 662
BccI CCATC 4 cut(s) 88, 125, 599, 721
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 1 cut(s) 85
BciVI GTATCC 1 cut(s) 519
BcoDI GTCTC 2 cut(s) 116, 538
BfaI CTAG 1 cut(s) 27
BfmI CTRYAG 2 cut(s) 438, 648
BfuAI ACCTGC 1 cut(s) 431
BfuI GTATCC 1 cut(s) 519
BisI GCNGC 5 cut(s) 193, 235, 238, 264, 651
BlpI GCTNAGC 1 cut(s) 143
BlsI GCNGC 5 cut(s) 194, 236, 239, 265, 652
Bme1390I CCNGG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 85
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 2 cut(s) 13, 709
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 4 cut(s) 126, 158, 325, 357
Bpu1102I GCTNAGC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 229, 241
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 202, 455
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 85
BseDI CCNNGG 2 cut(s) 229, 241
BseGI GGATG 5 cut(s) 4, 80, 93, 373, 610
BseLI CCNNNNNNNGG 3 cut(s) 84, 202, 455
BseMII CTCAG 3 cut(s) 157, 485, 614
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 4 cut(s) 27, 30, 33, 36
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 4 cut(s) 204, 224, 275, 662
BseYI CCCAGC 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 2 cut(s) 246, 708
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 216, 448
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 3 cut(s) 84, 202, 455
BsmAI GTCTC 2 cut(s) 116, 538
BsmFI GGGAC 1 cut(s) 610
BsnI GGCC 2 cut(s) 246, 708
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 216, 448
Bsp1720I GCTNAGC 1 cut(s) 143
BspACI CCGC 2 cut(s) 234, 462
BspANI GGCC 2 cut(s) 246, 708
BspCNI CTCAG 3 cut(s) 156, 484, 613
BspLI GGNNCC 1 cut(s) 460
BspMAI CTGCAG 2 cut(s) 442, 652
BspMI ACCTGC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 2 cut(s) 229, 241
BssSI CACGAG 1 cut(s) 613
BssT1I CCWWGG 2 cut(s) 229, 241
Bst2BI CACGAG 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 85
Bst4CI ACNGT 4 cut(s) 98, 103, 118, 281
Bst6I CTCTTC 2 cut(s) 161, 590
BstC8I GCNNGC 4 cut(s) 457, 521, 525, 688
BstDEI CTNAG 4 cut(s) 143, 471, 600, 641
BstF5I GGATG 5 cut(s) 4, 80, 93, 373, 610
BstMAI GTCTC 2 cut(s) 116, 538
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 5 cut(s) 243, 272, 452, 461, 719
BstNI CCWGG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 83
BstSFI CTRYAG 2 cut(s) 438, 648
BstSLI GKGCMC 1 cut(s) 216
BstV1I GCAGC 4 cut(s) 204, 224, 275, 662
BstV2I GAAGAC 1 cut(s) 183
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 2 cut(s) 246, 708
BtgZI GCGATG 1 cut(s) 144
BtsCI GGATG 5 cut(s) 4, 80, 93, 373, 610
BtsIMutI CAGTG 2 cut(s) 286, 345
BveI ACCTGC 1 cut(s) 431
Cac8I GCNNGC 4 cut(s) 457, 521, 525, 688
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 707
Csp6I GTAC 2 cut(s) 99, 560
CviAII CATG 3 cut(s) 22, 307, 520
CviQI GTAC 2 cut(s) 99, 560
DdeI CTNAG 4 cut(s) 143, 471, 600, 641
Eam1104I CTCTTC 2 cut(s) 161, 590
EarI CTCTTC 2 cut(s) 161, 590
Eco130I CCWWGG 2 cut(s) 229, 241
Eco147I AGGCCT 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 1 cut(s) 83
EcoT14I CCWWGG 2 cut(s) 229, 241
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 229, 241
FaeI CATG 3 cut(s) 25, 310, 523
FaiI YATR 8 cut(s) 23, 220, 288, 308, 422, 495, 521, 717
FaqI GGGAC 1 cut(s) 610
FatI CATG 3 cut(s) 21, 306, 519
Fnu4HI GCNGC 5 cut(s) 193, 235, 238, 264, 651
FokI GGATG 4 cut(s) 67, 100, 380, 617
Fsp4HI GCNGC 5 cut(s) 193, 235, 238, 264, 651
FspBI CTAG 1 cut(s) 27
GluI GCNGC 5 cut(s) 193, 235, 238, 264, 651
GsaI CCCAGC 1 cut(s) 270
HaeIII GGCC 2 cut(s) 246, 708
HapII CCGG 1 cut(s) 654
Hin1II CATG 3 cut(s) 25, 310, 523
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 5 cut(s) 107, 135, 400, 598, 733
HpaII CCGG 1 cut(s) 654
HphI GGTGA 2 cut(s) 24, 742
Hpy166II GTNNAC 4 cut(s) 32, 214, 327, 612
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 3 cut(s) 541, 602, 613
Hpy8I GTNNAC 4 cut(s) 32, 214, 327, 612
HpyAV CCTTC 2 cut(s) 674, 698
HpyCH4III ACNGT 4 cut(s) 98, 103, 118, 281
HpyCH4V TGCA 9 cut(s) 4, 114, 214, 356, 384, 440, 455, 650, 722
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 272, 452, 461, 719
HpyF3I CTNAG 4 cut(s) 143, 471, 600, 641
Hsp92II CATG 3 cut(s) 25, 310, 523
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 3 cut(s) 451, 464, 683
Lsp1109I GCAGC 4 cut(s) 204, 224, 275, 662
LweI GCATC 2 cut(s) 13, 709
MaeI CTAG 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 627
MboII GAAGA 6 cut(s) 148, 182, 185, 188, 426, 577
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 216, 448
MluCI AATT 3 cut(s) 360, 450, 746
MlyI GAGTC 3 cut(s) 116, 144, 592
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 1 cut(s) 85
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 85
MwoI GCNNNNNNNGC 5 cut(s) 243, 272, 452, 461, 719
NlaIII CATG 3 cut(s) 25, 310, 523
NlaIV GGNNCC 1 cut(s) 460
NmeAIII GCCGAG 1 cut(s) 130
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 523
OliI CACNNNNGTG 1 cut(s) 617
PaeI GCATGC 1 cut(s) 523
PaqCI CACCTGC 1 cut(s) 431
PceI AGGCCT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 400, 733
PfoI TCCNGGA 1 cut(s) 83
PkrI GCNGC 5 cut(s) 194, 236, 239, 265, 652
PleI GAGTC 3 cut(s) 115, 143, 592
PpsI GAGTC 3 cut(s) 115, 143, 592
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 83
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 1 cut(s) 707
PstI CTGCAG 2 cut(s) 442, 652
PvuII CAGCTG 1 cut(s) 266
RsaI GTAC 2 cut(s) 100, 561
RsaNI GTAC 2 cut(s) 99, 560
RseI CAYNNNNRTG 1 cut(s) 617
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 5 cut(s) 193, 235, 238, 264, 651
Sau96I GGNCC 1 cut(s) 707
SchI GAGTC 3 cut(s) 116, 144, 592
ScrFI CCNGG 1 cut(s) 85
SduI GDGCHC 2 cut(s) 216, 448
SfaNI GCATC 2 cut(s) 13, 709
SfcI CTRYAG 2 cut(s) 438, 648
SmiMI CAYNNNNRTG 1 cut(s) 617
SphI GCATGC 1 cut(s) 523
Sse9I AATT 3 cut(s) 360, 450, 746
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 2 cut(s) 234, 462
SspMI CTAG 1 cut(s) 27
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 83
StyI CCWWGG 2 cut(s) 229, 241
TaaI ACNGT 4 cut(s) 98, 103, 118, 281
TasI AATT 3 cut(s) 360, 450, 746
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 2 cut(s) 400, 733
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 286, 352
TseI GCWGC 4 cut(s) 192, 237, 263, 650
TspDTI ATGAA 1 cut(s) 747
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 2 cut(s) 286, 352
VneI GTGCAC 1 cut(s) 212
XapI RAATTY 1 cut(s) 746
XceI RCATGY 1 cut(s) 523
XspI CTAG 1 cut(s) 27
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.