pycom11g03020

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0006832
Physical Location & Seq
Reverse (-)
2375399 .. 2381911
6513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3294 bp
ATGGATACCAACTGGAGGCCTCCTCAAGGTGGAGAGCCTGCCATGGATGCCGGTGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACGTTGAAGAGGCATCTTCCCTTCTCCGGACAGGAGGGATTACTGGAACTCAAGAAAATTGCCGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGAAAATTTCGCTGAAGATGCTCACAATGGAGACCAAGTCTCAGAATTCAATGGCCAATTCCTTACAATCTAACTCTGCTGGTAACAGCAACAGGCCTCCGGATCCAGGGTCTTTTGCTATGCAACCCCAAGTCCCCAATCAAGGGCAATCACTCTCTATGCCGTTGCCAGCTAATCAATCTCAAGGACGCCAACAACTTCTATCACAGAACATCCAGAATAACGTTCCGCCTGCTGGAGTTCAAAGTTCGTCTGGCTTATCATCTGCACTACCTCCTTCTTCCGGTTTAACCCAGACTTCTATCCCAAGTATTGTTGGACAAAATCCAAACATGCAAAATATGTCTGGAAGTTCACAGAACTCATTAGGGAACTCCATGGGCCAGGGGGTACCCTCCAATATGTTTGCTAATTCAAGGCAAGTGCCAGGAAGGCAACAGGTAGTTCCCCAACAGCAGCAGCTGCAGCAATCCCAGAATTCGCAGCAATATGTGTACCCACAACAGCTGCAACATTCGCTTTTGAAGCCCAAATATCAGCAGGGAAATATGCCACAACTTGTGCAACAGCAACAGCAACAGCAACAGCAACAGCAGCAAAACCTATTACAACCGACTCAGCTGCAGTCTTCACAGCAATCTGTTATGCCAACATCATCTGTTATGCAGCCTTCGGTTCAATCATCGCTCTCGAGTCTTCAGCATAATCAACAATCTGTTATGCAACAGTCATCACAACCCATGCTTCAGCAGCATTCACAGCCAGTTCTCAGGCAGCAACAGCAGGCTCAACAGCCCGCTGTTCATCAACAGCAAACACCAATGCCACAACAGCCAATATTACCCCAGCAACAGCAGCAGCAGCCGCTTATGGTGCAACAGGCAACTGCTTCAAACTTGTCCCAGAATCAGATGATTGGACAACAAAACAATGTCGGTGATATGCAGCAGCAGCAGCAACAACAGAGGTTGCTAAGCCAGCAGAATAATCTTTTAAACCTGCAACAACAACAGCAGCAGCATCAACAGCAAAAGCAACAACACCAGCAGTTAATGGCTCTACAAAGCAACCCTTCTAATATGCATCAGCAACAATTGGGCCCTCAGAGTAATGTTACCGGGTTACAGCAACAGCAGCAAAGCAACCCTTCAAATATGCATCAGCAACAGTTGGGATCTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTCGGAACTCAAAGTGGTAATTCTAGCATGCAACCAAATCAGCACTCTGTACACTTGTTGCAGCAACAACAACATCAGAATGCATCTAACATGTTACCTTCTCAAGGACAGCAGTCACAACCTCAGGCATCCCAGATGCAACAAATGAGTTTGCAACAGCAGTCAAATCCACTGCAACGAGATATGCAACAAAGACTTCAAGCATCAGGTCAGATATCAGGAACCATGCTTCAATCACAAAACGTAATGGATCAGCAAAAGCAGTTATATCAAACTCAAAGAGCCCATCCTGAGACATCATCGACATCTCTAGATTCCACAGCTCAGACTGGACATGCAACTGGGGGTGATTGGCAAGAGGAGGTCTATCAAAAAATCAAAAGCATGAAGGAATTGTACTTTCCCGAACTAAATGAAATGTATCAGAAAATTGCTACCAAACTTCAGCAGCACGATTCTCTTCCACAACAACCAAAGTCAGAGCAGCTTGAGAAGCTAAAAATGTTCAAAACCATGTTGGAGCGCCTCATATCAGTCTTACAGATTCCCAAGAGTAACATTACACCTGGTTTGAAAGAGAAGTTGGGTACATACGAGAAGCAGATAGTAAATTTTATCAATACGAATAGACCGAGGAAGCAAGTTCCTCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGCACTCCATGCAGCAGCCGCAATCCCAAATTACTCAAGTGCAGTCGCATGAACATCAAATGAACCCGCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACAAGCTTGCAGCAAAATTCGATGTCTTCTATATCTGGGGTTTCAACAGCACAGCAGAACATGATGAATTCATTGCAGCCGAGTACAAATTTGGATTCTGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCCGGTGGGATCTATCCAACAAACTCCTGTCAGTGCTCCCCAGCAAGCAAACATGAATGCCTTGTCATCACAGAGTGGGGTTAGTATGCTGCAGCCAAATATTAATTCCCTTCAGTCAAATTCTGGTATGCTTCAACACCAGCAAATGAAACAGCAGGAACATCAAATGTTACAGAATCAACTGAAGCAACAGTATCAACAGCGAATGCAGCAGCAATTTATGCAGAAGCAGCAACAACAATTGCAGCAAGCAAAGCAACAGCTTCCGGCACAGATGCAGGTGCACCAACACCAAATGCCACAGCTTCATCAAATGAATGATGTAAATGACTTGAAGATGAGACAGGGGATGGGTGTTAAGCCAGGGGTTTTTCAGCAACATATGCCCGCAGGCCAGCGTGCTTATCCGCAACAGCAGTTGAAATCAGGATCTCCATTTCCTATTCCATCAACAAACCAACTCCTTCAGGCTGCATCTCCTCAAATTTCGCAACATTCTTCTCCCCAAGTTGACCAGCAGAATATACTGACTCACCCGAAAGCTGGAACCCCTTTGCAAACTGCTGGTTCACCTTTTGTCATCCCATCTCCTTCAACTCCCATGGCCCCATCCCCCATGCCAGGGGATTCTGAGAAACCTTCCTCACTATCGAATGCTGGGAATATTGGACATCAACAAGCAGCTGGGGTGGGAGCACCGGTCCAGTCCCTTGCAATTGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTAGTGTACCAGACGCTACTCATGTTAATGCTTTGTCAACTATTTCTGGCAAGTCGAGTGTTACCGAGCAGCCTCTTGAACGCTTGATTAAGGCGGTGAAATCGATGTCGCCTAATGCATTGAGTGCATCTGTCAGTGACATTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1098

Amino Acids

121.52

Weight (kDa)

9.71

Isoelectric Point (pI)

81.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 18 - 98 5e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2656
Acc36I ACCTGC 2 cut(s) 1239, 2656
Acc65I GGTACC 1 cut(s) 622
AccB1I GGYRCC 2 cut(s) 622, 3107
AccIII TCCGGA 2 cut(s) 140, 331
AciI CCGC 8 cut(s) 461, 1029, 1097, 2147, 2195, 2775, 2795, 3239
AclI AACGTT 1 cut(s) 456
AclWI GGATC 6 cut(s) 329, 342, 1414, 1704, 2404, 2824
AcoI YGGCCR 1 cut(s) 285
AcuI CTGAAG 7 cut(s) 266, 914, 962, 1874, 2483, 2591, 2837
AcyI GRCGYC 1 cut(s) 421
AdeI CACNNNGTG 1 cut(s) 2462
AfaI GTAC 7 cut(s) 624, 728, 1497, 1844, 2035, 2338, 3153
AflIII ACRYGT 1 cut(s) 1536
AgeI ACCGGT 1 cut(s) 3085
AjnI CCWGG 7 cut(s) 337, 615, 658, 2011, 2749, 3007, 3112
AjuI GAANNNNNNNTTGG 4 cut(s) 2012, 2044, 2476, 2508
Alw21I GWGCWC 3 cut(s) 2425, 2673, 3085
Alw26I GTCTC 4 cut(s) 257, 276, 1733, 2722
Alw44I GTGCAC 1 cut(s) 2669
AlwI GGATC 6 cut(s) 329, 342, 1414, 1704, 2404, 2824
AlwNI CAGNNNCTG 1 cut(s) 694
Ama87I CYCGRG 1 cut(s) 922
Aor13HI TCCGGA 2 cut(s) 140, 331
AoxI GGCC 7 cut(s) 17, 285, 326, 613, 1330, 2779, 2991
ApaI GGGCCC 1 cut(s) 1334
ApaLI GTGCAC 1 cut(s) 2669
AseI ATTAAT 1 cut(s) 2490
AsiGI ACCGGT 1 cut(s) 3085
Asp718I GGTACC 1 cut(s) 622
AspLEI GCGC 1 cut(s) 1971
AspS9I GGNCC 5 cut(s) 613, 1330, 1331, 2992, 3088
AsuC2I CCSGG 1 cut(s) 1351
AsuHPI GGTGA 6 cut(s) 65, 1181, 1805, 2912, 2949, 3253
AvaI CYCGRG 1 cut(s) 922
AvaII GGWCC 1 cut(s) 3088
AxyI CCTNAGG 1 cut(s) 1569
BaeGI GKGCMC 2 cut(s) 1334, 2673
BalI TGGCCA 1 cut(s) 287
BamHI GGATCC 1 cut(s) 334
BanI GGYRCC 2 cut(s) 622, 3107
BanII GRGCYC 2 cut(s) 1334, 1732
BarI GAAGNNNNNNTAC 2 cut(s) 1826, 1858
BbsI GAAGAC 3 cut(s) 852, 920, 2271
Bbv12I GWGCWC 3 cut(s) 2425, 2673, 3085
BccI CCATC 5 cut(s) 1740, 2731, 2842, 2980, 3004
BceAI ACGGC 2 cut(s) 161, 379
BcgI CGANNNNNNTGC 2 cut(s) 835, 869
BciT130I CCWGG 7 cut(s) 339, 617, 660, 2013, 2751, 3009, 3114
BciVI GTATCC 1 cut(s) 106
BcnI CCSGG 1 cut(s) 1351
BcoDI GTCTC 4 cut(s) 257, 276, 1733, 2722
BfaI CTAG 2 cut(s) 1470, 1757
BfmI CTRYAG 5 cut(s) 71, 204, 695, 854, 2477
BfoI RGCGCY 1 cut(s) 1972
BfuAI ACCTGC 2 cut(s) 1239, 2656
BfuI GTATCC 1 cut(s) 106
BglI GCCNNNNNGGC 1 cut(s) 664
BlpI GCTNAGC 1 cut(s) 1205
Bme1390I CCNGG 8 cut(s) 339, 617, 660, 1351, 2013, 2751, 3009, 3114
Bme18I GGWCC 1 cut(s) 3088
BmeT110I CYCGRG 1 cut(s) 922
BmgT120I GGNCC 5 cut(s) 613, 1330, 1331, 2992, 3088
BmiI GGNNCC 7 cut(s) 336, 624, 1332, 1669, 2935, 2994, 3109
BmrFI CCNGG 8 cut(s) 339, 617, 660, 1351, 2013, 2751, 3009, 3114
BmrI ACTGGG 1 cut(s) 1797
BmuI ACTGGG 1 cut(s) 1797
BoxI GACNNNNGTC 1 cut(s) 1558
BpiI GAAGAC 3 cut(s) 852, 920, 2271
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 2 cut(s) 34, 489
Bpu1102I GCTNAGC 1 cut(s) 1205
BpuEI CTTGAG 6 cut(s) 9, 149, 399, 1533, 1955, 2148
BpuMI CCSGG 1 cut(s) 1351
Bsa29I ATCGAT 1 cut(s) 3248
BsaHI GRCGYC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 257
BsaWI WCCGGW 5 cut(s) 140, 331, 515, 2389, 3085
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 3072, 3102
Bse118I RCCGGY 2 cut(s) 50, 3085
Bse1I ACTGG 8 cut(s) 17, 162, 217, 995, 1429, 1780, 1792, 3091
Bse21I CCTNAGG 1 cut(s) 1569
Bse3DI GCAATG 1 cut(s) 2324
BseAI TCCGGA 2 cut(s) 140, 331
BseBI CCWGG 7 cut(s) 339, 617, 660, 2013, 2751, 3009, 3114
BseCI ATCGAT 1 cut(s) 3248
BseGI GGATG 7 cut(s) 52, 444, 1574, 1732, 2742, 2967, 2996
BseMI GCAATG 1 cut(s) 2324
BseMII CTCAG 9 cut(s) 287, 863, 1015, 1349, 1424, 1583, 1728, 1784, 3009
BseNI ACTGG 8 cut(s) 17, 162, 217, 995, 1429, 1780, 1792, 3091
BseRI GAGGAG 5 cut(s) 12, 76, 1820, 2085, 2856
BseSI GKGCMC 2 cut(s) 1334, 2673
BseYI CCCAGC 4 cut(s) 1077, 2427, 3044, 3071
BsgI GTGCAG 2 cut(s) 483, 2189
BshFI GGCC 7 cut(s) 19, 287, 328, 615, 1332, 2781, 2993
BshNI GGYRCC 2 cut(s) 622, 3107
BshTI ACCGGT 1 cut(s) 3085
BshVI ATCGAT 1 cut(s) 3248
BsiHKAI GWGCWC 3 cut(s) 2425, 2673, 3085
BsiHKCI CYCGRG 1 cut(s) 922
BsiSI CCGG 8 cut(s) 51, 141, 332, 516, 1350, 2390, 2654, 3086
BslFI GGGAC 3 cut(s) 350, 1117, 3079
BsmAI GTCTC 4 cut(s) 257, 276, 1733, 2722
BsmFI GGGAC 3 cut(s) 350, 1117, 3079
BsmI GAATGC 5 cut(s) 985, 1531, 2449, 2598, 3046
BsnI GGCC 7 cut(s) 19, 287, 328, 615, 1332, 2781, 2993
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 922
Bsp120I GGGCCC 1 cut(s) 1330
Bsp1286I GDGCHC 5 cut(s) 1334, 1732, 2425, 2673, 3085
Bsp13I TCCGGA 2 cut(s) 140, 331
Bsp1407I TGTACA 1 cut(s) 1495
Bsp143I GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
Bsp1720I GCTNAGC 1 cut(s) 1205
Bsp19I CCATGG 3 cut(s) 42, 609, 2988
BspACI CCGC 8 cut(s) 461, 1029, 1097, 2147, 2195, 2775, 2795, 3239
BspANI GGCC 7 cut(s) 19, 287, 328, 615, 1332, 2781, 2993
BspCNI CTCAG 9 cut(s) 286, 862, 1014, 1348, 1423, 1582, 1729, 1783, 3010
BspDI ATCGAT 1 cut(s) 3248
BspEI TCCGGA 2 cut(s) 140, 331
BspLI GGNNCC 7 cut(s) 336, 624, 1332, 1669, 2935, 2994, 3109
BspMAI CTGCAG 5 cut(s) 75, 208, 699, 858, 2481
BspMI ACCTGC 2 cut(s) 1239, 2656
BspPI GGATC 6 cut(s) 329, 342, 1414, 1704, 2404, 2824
BspT107I GGYRCC 2 cut(s) 622, 3107
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 2324
BsrFI RCCGGY 2 cut(s) 50, 3085
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 8 cut(s) 17, 162, 217, 995, 1429, 1780, 1792, 3091
BssAI RCCGGY 2 cut(s) 50, 3085
BssMI GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
BssNI GRCGYC 1 cut(s) 421
BssT1I CCWWGG 3 cut(s) 42, 609, 2988
Bst2UI CCWGG 7 cut(s) 339, 617, 660, 2013, 2751, 3009, 3114
Bst4CI ACNGT 3 cut(s) 960, 1401, 2580
Bst6I CTCTTC 2 cut(s) 116, 1911
BstACI GRCGYC 1 cut(s) 421
BstAPI GCANNNNNTGC 5 cut(s) 694, 2137, 2608, 2770, 3269
BstAUI TGTACA 1 cut(s) 1495
BstDSI CCRYGG 3 cut(s) 42, 609, 2988
BstENI CCTNNNNNAGG 2 cut(s) 24, 1548
BstF5I GGATG 7 cut(s) 52, 444, 1574, 1732, 2742, 2967, 2996
BstH2I RGCGCY 1 cut(s) 1972
BstHHI GCGC 1 cut(s) 1971
BstKTI GATC 5 cut(s) 337, 1409, 1699, 2399, 2819
BstMAI GTCTC 4 cut(s) 257, 276, 1733, 2722
BstMBI GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
BstNI CCWGG 7 cut(s) 339, 617, 660, 2013, 2751, 3009, 3114
BstNSI RCATGY 4 cut(s) 568, 1477, 1540, 1784
BstPAI GACNNNNGTC 1 cut(s) 1558
BstSCI CCNGG 8 cut(s) 337, 615, 658, 1349, 2011, 2749, 3007, 3112
BstSFI CTRYAG 5 cut(s) 71, 204, 695, 854, 2477
BstSLI GKGCMC 2 cut(s) 1334, 2673
BstV2I GAAGAC 3 cut(s) 852, 920, 2271
BstX2I RGATCY 4 cut(s) 334, 1406, 2396, 2816
BstYI RGATCY 4 cut(s) 334, 1406, 2396, 2816
Bsu15I ATCGAT 1 cut(s) 3248
Bsu36I CCTNAGG 1 cut(s) 1569
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 7 cut(s) 19, 287, 328, 615, 1332, 2781, 2993
BsuTUI ATCGAT 1 cut(s) 3248
BtgI CCRYGG 3 cut(s) 42, 609, 2988
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 7 cut(s) 52, 444, 1574, 1732, 2742, 2967, 2996
BtsI GCAGTG 1 cut(s) 1616
BtsIMutI CAGTG 4 cut(s) 1616, 2366, 2425, 3286
BveI ACCTGC 2 cut(s) 1239, 2656
CaiI CAGNNNCTG 1 cut(s) 694
CfoI GCGC 1 cut(s) 1971
Cfr10I RCCGGY 2 cut(s) 50, 3085
Cfr13I GGNCC 5 cut(s) 613, 1330, 1331, 2992, 3088
ClaI ATCGAT 1 cut(s) 3248
CseI GACGC 2 cut(s) 429, 3167
CsiI ACCWGGT 1 cut(s) 2011
Csp6I GTAC 7 cut(s) 623, 727, 1496, 1843, 2034, 2337, 3152
CspAI ACCGGT 1 cut(s) 3085
CviQI GTAC 7 cut(s) 623, 727, 1496, 1843, 2034, 2337, 3152
DpnI GATC 5 cut(s) 336, 1408, 1698, 2398, 2818
DpnII GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
DraI TTTAAA 1 cut(s) 1227
DraIII CACNNNGTG 1 cut(s) 2462
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 116, 1911
EarI CTCTTC 2 cut(s) 116, 1911
EciI GGCGGA 1 cut(s) 450
Eco130I CCWWGG 3 cut(s) 42, 609, 2988
Eco147I AGGCCT 2 cut(s) 19, 328
Eco24I GRGCYC 2 cut(s) 1334, 1732
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 2 cut(s) 1662, 3120
Eco47I GGWCC 1 cut(s) 3088
Eco57I CTGAAG 7 cut(s) 266, 914, 962, 1874, 2483, 2591, 2837
Eco81I CCTNAGG 1 cut(s) 1569
Eco88I CYCGRG 1 cut(s) 922
EcoNI CCTNNNNNAGG 2 cut(s) 24, 1548
EcoO109I RGGNCCY 1 cut(s) 1331
EcoRI GAATTC 3 cut(s) 277, 709, 2320
EcoRII CCWGG 7 cut(s) 337, 615, 658, 2011, 2749, 3007, 3112
EcoRV GATATC 2 cut(s) 1662, 3120
EcoT14I CCWWGG 3 cut(s) 42, 609, 2988
EcoT22I ATGCAT 4 cut(s) 1317, 1392, 1531, 3265
EcoT38I GRGCYC 2 cut(s) 1334, 1732
ErhI CCWWGG 3 cut(s) 42, 609, 2988
FalI AAGNNNNNCTT 4 cut(s) 1288, 1320, 1363, 1395
FaqI GGGAC 3 cut(s) 350, 1117, 3079
FauI CCCGC 3 cut(s) 1036, 2202, 2782
FauNDI CATATG 2 cut(s) 2127, 2769
FokI GGATG 7 cut(s) 59, 431, 1561, 1719, 2749, 2954, 2983
FriOI GRGCYC 2 cut(s) 1334, 1732
FspBI CTAG 2 cut(s) 1470, 1757
GlaI GCGC 1 cut(s) 1970
GsaI CCCAGC 4 cut(s) 1081, 2431, 3048, 3075
GsuI CTGGAG 2 cut(s) 34, 489
HaeII RGCGCY 1 cut(s) 1972
HaeIII GGCC 7 cut(s) 19, 287, 328, 615, 1332, 2781, 2993
HapII CCGG 8 cut(s) 51, 141, 332, 516, 1350, 2390, 2654, 3086
HgaI GACGC 2 cut(s) 429, 3167
HhaI GCGC 1 cut(s) 1971
Hin1I GRCGYC 1 cut(s) 421
Hin6I GCGC 1 cut(s) 1969
HinP1I GCGC 1 cut(s) 1969
HincII GTYRAC 3 cut(s) 100, 2899, 3183
HindII GTYRAC 3 cut(s) 100, 2899, 3183
HindIII AAGCTT 1 cut(s) 2257
HpaII CCGG 8 cut(s) 51, 141, 332, 516, 1350, 2390, 2654, 3086
HphI GGTGA 6 cut(s) 65, 1181, 1805, 2912, 2949, 3253
Hpy166II GTNNAC 9 cut(s) 100, 587, 727, 1498, 2671, 2899, 2957, 3152, 3183
Hpy8I GTNNAC 9 cut(s) 100, 587, 727, 1498, 2671, 2899, 2957, 3152, 3183
HpyCH4III ACNGT 3 cut(s) 960, 1401, 2580
HpyCH4IV ACGT 3 cut(s) 116, 456, 1689
HpySE526I ACGT 3 cut(s) 116, 456, 1689
Hsp92I GRCGYC 1 cut(s) 421
HspAI GCGC 1 cut(s) 1969
Kpn2I TCCGGA 2 cut(s) 140, 331
KpnI GGTACC 1 cut(s) 626
Kzo9I GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
LmnI GCTCC 4 cut(s) 1966, 2118, 2428, 3080
MabI ACCWGGT 1 cut(s) 2011
MaeI CTAG 2 cut(s) 1470, 1757
MaeII ACGT 3 cut(s) 116, 456, 1689
MalI GATC 5 cut(s) 336, 1408, 1698, 2398, 2818
MboI GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
MfeI CAATTG 3 cut(s) 1325, 2627, 3102
MflI RGATCY 4 cut(s) 334, 1406, 2396, 2816
MhlI GDGCHC 5 cut(s) 1334, 1732, 2425, 2673, 3085
MlsI TGGCCA 1 cut(s) 287
MluNI TGGCCA 1 cut(s) 287
MlyI GAGTC 4 cut(s) 73, 841, 934, 2911
MmeI TCCRAC 4 cut(s) 201, 529, 1944, 2428
Mox20I TGGCCA 1 cut(s) 287
Mph1103I ATGCAT 4 cut(s) 1317, 1392, 1531, 3265
MroI TCCGGA 2 cut(s) 140, 331
MscI TGGCCA 1 cut(s) 287
MseI TTAA 7 cut(s) 521, 1226, 1283, 2490, 2745, 3171, 3234
MslI CAYNNNNRTG 2 cut(s) 1524, 3171
Msp20I TGGCCA 1 cut(s) 287
MspA1I CMGCKG 6 cut(s) 70, 694, 739, 853, 1031, 3071
MspI CCGG 8 cut(s) 51, 141, 332, 516, 1350, 2390, 2654, 3086
MspR9I CCNGG 8 cut(s) 339, 617, 660, 1351, 2013, 2751, 3009, 3114
MunI CAATTG 3 cut(s) 1325, 2627, 3102
Mva1269I GAATGC 5 cut(s) 985, 1531, 2449, 2598, 3046
MvaI CCWGG 7 cut(s) 339, 617, 660, 2013, 2751, 3009, 3114
NciI CCSGG 1 cut(s) 1351
NcoI CCATGG 3 cut(s) 42, 609, 2988
NdeI CATATG 2 cut(s) 2127, 2769
NdeII GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
NlaIV GGNNCC 7 cut(s) 336, 624, 1332, 1669, 2935, 2994, 3109
NmeAIII GCCGAG 1 cut(s) 2358
NmuCI GTSAC 2 cut(s) 1560, 3281
NsiI ATGCAT 4 cut(s) 1317, 1392, 1531, 3265
NspI RCATGY 4 cut(s) 568, 1477, 1540, 1784
PaeI GCATGC 1 cut(s) 1477
PaeR7I CTCGAG 1 cut(s) 922
PaqCI CACCTGC 1 cut(s) 2656
PasI CCCWGGG 1 cut(s) 3113
PceI AGGCCT 2 cut(s) 19, 328
PciI ACATGT 1 cut(s) 1536
PcsI WCGNNNNNNNCGW 1 cut(s) 2075
PctI GAATGC 5 cut(s) 985, 1531, 2449, 2598, 3046
PfeI GAWTC 8 cut(s) 80, 1138, 1760, 1901, 1990, 2348, 2563, 3014
PflFI GACNNNGTC 1 cut(s) 268
PinAI ACCGGT 1 cut(s) 3085
PleI GAGTC 4 cut(s) 72, 841, 933, 2911
PpsI GAGTC 4 cut(s) 72, 841, 933, 2911
PscI ACATGT 1 cut(s) 1536
PshAI GACNNNNGTC 1 cut(s) 1558
PshBI ATTAAT 1 cut(s) 2490
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 7 cut(s) 337, 615, 658, 2011, 2749, 3007, 3112
PspFI CCCAGC 4 cut(s) 1077, 2427, 3044, 3071
PspGI CCWGG 7 cut(s) 337, 615, 658, 2011, 2749, 3007, 3112
PspN4I GGNNCC 7 cut(s) 336, 624, 1332, 1669, 2935, 2994, 3109
PspOMI GGGCCC 1 cut(s) 1330
PspPI GGNCC 5 cut(s) 613, 1330, 1331, 2992, 3088
PstI CTGCAG 5 cut(s) 75, 208, 699, 858, 2481
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 4 cut(s) 334, 1406, 2396, 2816
PsyI GACNNNGTC 1 cut(s) 268
PvuII CAGCTG 5 cut(s) 70, 694, 739, 853, 3071
RsaI GTAC 7 cut(s) 624, 728, 1497, 1844, 2035, 2338, 3153
RsaNI GTAC 7 cut(s) 623, 727, 1496, 1843, 2034, 2337, 3152
RseI CAYNNNNRTG 2 cut(s) 1524, 3171
SaqAI TTAA 7 cut(s) 521, 1226, 1283, 2490, 2745, 3171, 3234
Sau3AI GATC 5 cut(s) 334, 1406, 1696, 2396, 2816
Sau96I GGNCC 5 cut(s) 613, 1330, 1331, 2992, 3088
SchI GAGTC 4 cut(s) 73, 841, 934, 2911
ScrFI CCNGG 8 cut(s) 339, 617, 660, 1351, 2013, 2751, 3009, 3114
SduI GDGCHC 5 cut(s) 1334, 1732, 2425, 2673, 3085
SexAI ACCWGGT 1 cut(s) 2011
SfcI CTRYAG 5 cut(s) 71, 204, 695, 854, 2477
Sfr274I CTCGAG 1 cut(s) 922
SinI GGWCC 1 cut(s) 3088
SlaI CTCGAG 1 cut(s) 922
SmiMI CAYNNNNRTG 2 cut(s) 1524, 3171
SmlI CTYRAG 7 cut(s) 24, 164, 414, 922, 1548, 1934, 2163
SmoI CTYRAG 7 cut(s) 24, 164, 414, 922, 1548, 1934, 2163
SphI GCATGC 1 cut(s) 1477
SseBI AGGCCT 2 cut(s) 19, 328
SsiI CCGC 8 cut(s) 461, 1029, 1097, 2147, 2195, 2775, 2795, 3239
SspI AATATT 3 cut(s) 1071, 2488, 3052
SspMI CTAG 2 cut(s) 1470, 1757
StuI AGGCCT 2 cut(s) 19, 328
StyD4I CCNGG 8 cut(s) 337, 615, 658, 1349, 2011, 2749, 3007, 3112
StyI CCWWGG 3 cut(s) 42, 609, 2988
TaaI ACNGT 3 cut(s) 960, 1401, 2580
TaiI ACGT 3 cut(s) 119, 459, 1692
TaqI TCGA 6 cut(s) 923, 1748, 2273, 3038, 3200, 3248
TaqII GACCGA 1 cut(s) 2092
TatI WGTACW 3 cut(s) 1495, 1842, 2336
TauI GCSGC 2 cut(s) 1099, 2149
TfiI GAWTC 8 cut(s) 80, 1138, 1760, 1901, 1990, 2348, 2563, 3014
Tru1I TTAA 7 cut(s) 521, 1226, 1283, 2490, 2745, 3171, 3234
Tru9I TTAA 7 cut(s) 521, 1226, 1283, 2490, 2745, 3171, 3234
TscAI CASTG 4 cut(s) 1623, 2373, 2425, 3286
TseFI GTSAC 2 cut(s) 1560, 3281
Tsp45I GTSAC 2 cut(s) 1560, 3281
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 4 cut(s) 1623, 2373, 2425, 3286
Tth111I GACNNNGTC 1 cut(s) 268
VneI GTGCAC 1 cut(s) 2669
VpaK11BI GGWCC 1 cut(s) 3088
VspI ATTAAT 1 cut(s) 2490
XagI CCTNNNNNAGG 2 cut(s) 24, 1548
XbaI TCTAGA 1 cut(s) 1756
XceI RCATGY 4 cut(s) 568, 1477, 1540, 1784
XhoI CTCGAG 1 cut(s) 922
XspI CTAG 2 cut(s) 1470, 1757
Zsp2I ATGCAT 4 cut(s) 1317, 1392, 1531, 3265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.